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cholesterol inhibition of isopentenyl pyrophosphate delta3, delta2-isomerase in mycoplasma laidlawii.cholesterol inhibits isopentenyl delta(3),delta(2)-isomerase of mycoplasma laidlawii in an apparently competitive fashion. the conversion of mevalonic acid to isopentenyl pyrophosphate is slightly stimulated. organisms grown in the presence of mevalonic-2-(14)c acid contain small amounts of radio-label in nucleic acid and protein fractions. most of the label is found in the lipids and is reduced dramatically in organisms grown with cholesterol. no significant accumulation of phosphorylated inter ...19704316364
the synthesis of long-chain fatty acids by a cell-free system from mycoplasma laidlawii a. 19704391501
membrane structure: spin labeling and freeze etching of mycoplasma laidawii.a spin-labeled fatty acid was incorporated in vivo into the polar lipids of mycoplasma laidlawii membranes. the electron paramagnetic resonance signal from either intact cells or their extracted lipids reflected the fatty acid composition of the mycoplasma membranes. comparison of signals from intact cells, gramicidin-treated cells, heat-treated cells, and extracted lipids indicates that a major portion of the membrane lipids is in a semiviscous hydrocarbon environment. the results also show tha ...19704316683
immunoelectrophoretic demonstration of proteolytic activity against human proteins by mycoplasma laidlawii. 19704248067
d- and l-alanylphosphatidylglycerols from mycoplasma laidlawii, strain b. 19694391533
synthesis and turnover of membrane protein and lipid in mycoplasma laidlawii. 19694307353
observations on membranes of mycoplasma laidlawii strain b.the cytoplasmic membrane of mycoplasma laidlawii strain b is solubilized by anionic and nonionic detergents, succinylation, phospholipase a, alkaline phosphatase, trypsin, and chymotrypsin. cationic detergents are without effect, as are chelating agents, even in the presence of high concentrations of monovalent cation. the detergent-solubilized membrane exhibits one peak in the analytical ultracentrifuge, but the sedimentation coefficient is dependent upon concentration of detergent. simple dial ...19695361209
deoxyribonucleic acid homology and relative genome size in mycoplasma.the deoxyribonucleic acid homologies of mycoplasma laidlawii type a and type b, m. pulmonis (#47 and #63), and m. hominis were determined by membrane methodology. the homology data revealed a difference in genome size between m. laidlawii type a and type b. this difference also held with stringent conditions of annealing (high temperature). little or negligible homology was shown to exist between the m. laidlawii strains type a and type b and m. pulmonis strains 47 and 63 and m. hominis. m. homi ...19695370271
the effect of long chain fatty acid isomers on growth, fatty acid composition and osmotic fragility of mycoplasma laidlawii a. 19695374018
proposal for classifying organisms related to mycoplasma laidlawii in a family sapromycetaceae, genus sapromyces, within the mycoplasmatales. 19695391433
new sero-types of mycoplasma laidlawii isolated from mastitic milk and urogenital tracts of cattle. ii. typing of m. laidlawii by biological and serological methods. 19695392349
new sero-types of mycoplasma laidlawii isolated from mastitic milk and urogenital tracts of cattle. i. isolation of m. laidlawii. 19695817669
repair replication of dna in ultraviolet irradiated mycoplasma laidlawii b. 19695358643
mycoplasma membrane lipids: variations in fatty acid composition.the fatty acid composition of the membrane polar lipids of mycoplasma laidlawii b can be dramatically altered. these variations reslut in characteristic morphological chlanges, and in most cases the cells remain viable. this organism should provide a useful system for clarifying the role of fatty acyl chains in biological membranes.19695777213
mycoplasma laidlawii in human burns.mycoplasma laidlawii was recovered from burns from 13 of 52 patients. in most cases, cocci or bacilli were isolated simultaneously.19695784230
characterization of the plasma membrane of mycoplasma laidlawii. v. effects of selective removal of protein and lipid. 19695792241
characterization of the plasma membrane of mycoplasma laidlawii. vi. potassium transport. 19695792242
biosynthesis of glucosyl diglycerides by mycoplasma laidlawii strain b.monoglucosyl diglyceride is synthesized from 1,2-diglyceride and uridine-5'-diphosphoglucose (udp); diglucosyl diglyceride from monoglucosyl diglyceride, and uridine-5'-diphosphoglucose by membranes of mycoplasma laidlawii strain b. all of these enzymatic activities reside in the membrane. membranes solubilized by detergent action or succinylation and acetone powders of membranes were inactive. requirements for mg(2+), udp, and appropriate lipid acceptor were demonstrated for biosynthesis of bot ...19695808075
the role of lipids in membrane transport in mycoplasma laidlawii. 19695823712
calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane.both membranes of mycoplasma laidlawii and water dispersions of protein-free membrane lipids exhibit thermal phase transitions that can be detected by differential scanning calorimetry. the transition temperatures are lowered by increased unsaturation in the fatty acid residues, but in each case they are the same for membranes and lipids. the transitions resemble those observed for synthetic lipids in the lamellar phase in water, which arise from melting of the hydrocarbon chains within the phos ...19695257955
immunological analysis of mycoplasma membranes.the antigens responsible for the production of antibodies to mycoplasma laidlawii and m. gallisepticum causing growth and metabolic inhibition of these organisms were localized in the cell membrane. various membrane fractions were tested for serological activity. membrane lipids were completely or almost completely inactive, whereas several preparations of defatted membrane proteins retained some serological activity, shown by their ability to stimulate metabolic inhibition antibody in rabbits a ...19694981055
dna replication in mycoplasma laidlawii b. 19694890600
rapid determination by light scattering of growth parameters of mycoplasma laidlawii in liquid media.an impediment to progress in the study of the course of growth, the effects of medium components, antibiotics, etc., of mycoplasma has been the cumbersome methods of growth measurement currently in use. heretofore, it required the plating of numerous samples during growth, at least in triplicate, after appropriate dilution, followed by a delay of 2 to 3 days before the colonies developed so that counts could be made. we applied the technique of light scattering to measure the growth of mycoplasm ...19694896103
characteristics of a new sterol-nonrequiring mycoplasma.two mycoplasma strains recovered from tissue culture environments were found to grow in complex media devoid of serum or serum fractions containing cholesterol and in a cholesterol-free synthetic medium. neither strain was capable of synthesizing pigmented carotenoids, although these compounds are present in, and characteristic of, other sterol-nonrequiring mycoplasmas. serological tests and an analysis of their cell protein patterns obtained by gel electrophoresis indicated that the isolates we ...19694892385
an ultrastructural study of mycoplasma laidlawii type a and two strains of mycoplasma laidlawii isolated from the oviducts of dairy cows. 19684888099
deoxyribonucleate binding and transformation in mycoplasma laidlawii. 19684865478
physiological and serological comparisons among strains of mycoplasma granularum and mycoplasma laidlawii.mycoplasma granularum strains grew on a medium devoid of animal serum or of serum fractions containing sterols; all strains possessed properties, including carotenoid biosynthesis, similar to those described for m. laidlawii. some common antigenic components were noted among m. granularum and m. laidlawii strains by indirect fluorescent-antibody tests. the growth of m. granularum strains was slightly inhibited by antiserum to m. laidlawii pg-8, and the electrophoretic patterns of cell proteins o ...19684870272
macromolecular synthesis and thymineless death in mycoplasma laidlawii b.the relationships between macromolecular synthesis and viability have been studied in the pleuropneumonia-like organism mycoplasma laidlawii b adapted to a semidefined grwoth medium. this organism exhibited an absolute growth requirement for the nucleosides uridine and thymidine, a partial requirement for guanosine and deoxyguanosine, but no requirement for adenosine, deoxyadenosine, cytosine, and deoxycytosine. cytosine and deoxycytosine partially satisfied the requirement for uridine. loss in ...19684881702
characterization of the plasma membrane of mycoplasma laidlawii. iv. structure and composition of membrane and aggregated components. 19685650391
characterization of the plasma membrane of mycoplasma laidlawii. 3. the formation and aggregation of small lipoprotein structures derived from sodium dodecyl sulfate-solubilized membrane components. 19685650390
the lipid composition of mycoplasma laidlawii strain b.1. total lipid was extracted from mycoplasma laidlawii strain b with chloroform-methanol mixtures and fractionated into neutral lipid, glycolipid and phospholipid components by chromatography on silicic acid. 2. saponification of the glycolipid fraction, which represented nearly half of the total lipid, yielded two glycosides for which the structures o-alpha-d-glucopyranosyl-(1-->1)-d-glycerol and o-alpha-d-glucopyranosyl-(1-->2)-o-alpha-d-glucopyranosyl-(1-->1)-d-glycerol were established. 3. t ...19684297044
carotenoids as protectors against photodynamic inactivation of the adenosine triphosphatase of mycoplasma laidlawii membranes. 19684234699
control of nadh oxidation by adp in membranes from mycoplasma laidlawii. 19674294270
characterization of the plasma membrane of mycoplasma laidlawii. ii. modes of aggregation of solubilized membrane components. 19676058126
role of carotenoids and cholesterol in the growth of mycoplasma laidlawii. 19676025421
a comparison of the osmotic and passive permeability properties of mycoplasma laidlawii and mycoplasma hominis. 19676035538
characterization of the plasma membrane of mycoplasma laidlawii. i. sodium dodecyl sulfate solubilization. 19676048810
glucolipids from mycoplasma laidlawii and streptococcus mg. 19676049904
influence of lipid components of mycoplasma laidlawii membranes on osmotic fragility of cells.razin, s. (university of connecticut, storrs), m. e. tourtellotte, r. n. mcelhaney, and j. d. pollack. influence of lipid components of mycoplasma laidlawii membranes on osmotic fragility of cells. j. bacteriol. 91:609-616. 1966.-lipid composition of mycoplasma laidlawii membranes could be significantly changed by variations in the growth medium. the effect of these changes on the osmotic fragility of the cells was studied. cholesterol, incorporated into the membrane from the growth medium, had ...19665883100
growth inhibition of mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol.smith, paul f. (university of south dakota, vermillion), and carl v. henrikson. growth inhibition of mycoplasma by inhibitors of polyterpene biosynthesis and its reversal by cholesterol. j. bacteriol. 91:1854-1858. 1966.-compounds which inhibit enzymatic reactions in the biosynthetic pathway to carotenoids inhibited growth of a sterol-nonrequiring species, mycoplasma laidlawii, strain b, and m. hominis, strain 07. since m. hominis lacks the enzymes for polyterpene biosynthesis, the inhibitory co ...19665937241
adenosine triphosphatase activity of mycoplasma membranes.rottem, shlomo (hebrew university, jerusalem, israel), and shmuel razin. adenosine triphosphatase activity of mycoplasma membranes. j. bacteriol. 92:714-722. 1966.-adenosine triphosphatase activity of mycoplasma laidlawii, m. gallisepticum, and mycoplasma sp. strain 14 was confined to the cell membrane. the enzymatic activity was dependent on magnesium, but was not activated by sodium and potassium. ouabain did not inhibit the adenosine triphosphatase activity of the mycoplasmas, and did not int ...19664224219
carotenoids and cholesterol in membranes of mycoplasma laidlawii. 19655882653
morphology of mycoplasma laidlawii type a. ii. effect of glucose on growth and cellular morphology.anderson, d. l. (university of minnesota, minneapolis), m. e. pollock, and l. f. brower. morphology of mycoplasma laidlawii type a. ii. effect of glucose on growth and cellular morphology. j. bacteriol. 90:1768-1777. 1965.-cells of mycoplasma laidlawii a grown in soy peptone-yeast extract (sp-ye) broth reached higher maximal titers than cells grown in sp-ye supplemented with 0.2% glucose, and remained viable longer than glucose-grown cells. moreover, addition of glucose to sp-ye markedly affecte ...19655854595
use of dialyzing culture technique for high yield of mycoplasma.pollock, mary e. (university of minnesota, minneapolis). use of dialyzing culture technique for high yield of mycoplasma. j. bacteriol. 90:1682-1685. 1965.-the saprophytic pleuropneumonia-like organism, mycoplasma laidlawii, type a, was cultivated within a dialyzing membrane suspended in a reservoir of uninoculated medium. generation time was not decreased by this method, but the period of active growth was prolonged, and the final yield was 10-fold that attained by the usual cultural methods. a ...19655322724
morphology of mycoplasma laidlawii type a. i. comparison of electron microscopic counts with colony-forming units.anderson, d. l. (university of minnesota, minneapolis), m. e. pollock, and l. f. brower. morphology of mycoplasma laidlawii type a. i. comparison of electron microscopic counts with colony-forming units. j. bacteriol. 90:1764-1767. 1965.-cells of mycoplasma laidlawii a grown in dialyzing flask cultures were counted with the electron microscope by use of a spray technique which deposited mixtures of polystyrene latex of known concentration and m. laidlawii in discrete droplet patterns on specimen ...19655322728
membrane subunits of mycoplasma laidlawii and their assembly to membranelike structures. 19655216355
effect of cholesterol on the sensitivity of mycoplasma laidlawii to the polyene antibiotic filipin.weber, morton m. (st. louis university school of medicine, st. louis, mo.), and stephen c. kinsky. effect of cholesterol on the sensitivity of mycoplasma laidlawii to the polyene antibiotic filipin. j. bacteriol. 89:306-312. 1965.-the polyene antibiotic, filipin, inhibited growth and caused lysis of mycoplasma laidlawii cells which had been cultured in the presence of cholesterol. the antibiotic did not inhibit growth and did not promote lysis of the organism when grown in the absence of cholest ...196514255695
glucose-containing phospholipids in mycoplasma laidlawii, strain b. 196514280456
the action of amphotericin b on mycoplasma laidlawii. 196514332454
diphosphatidyl glycerol in mycoplasma laidlawii. 196516562032
defined medium for mycoplasma laidlawii.tourtellotte, mark e. (university of connecticut, storrs), harold j. morowitz, and phil kasimer. defined medium for mycoplasma laidlawii. j. bacteriol. 88:11-15. 1964.-a defined medium for the pleuropneumonia-like organism mycoplasma laidlawii b is described in which absolute requirements for coenzyme a and longchain fatty acids were demonstrated. this organism did not require cholesterol or macromolecules of high molecular weight, but did show a growth requirement for peptides. optimal growth i ...196414197875
mycoplasma laidlawii var. inocuum comb. nov. 19645874544
glucose metabolism of two strains of mycoplasma laidlawii.castrejon-diez, jaime (tulane university school of medicine, new orleans, la.), thelma n. fisher, and earl fisher, jr. glucose metabolism of two strains of mycoplasma laidlawii. j. bacteriol. 86:627-636. 1963.-two strains of mycoplasma laidlawii were incubated in systems containing d-glucose-c(14); carbon dioxide, acetate, pyruvate, and lactate were isolated from appropriate fluids after resting-cell and growth experiments. in resting-cell experiments, radioactivity recoveries were shown to be 9 ...196314066454
fatty acid requirements of mycoplasma laidlawii. 196314114935
nutritional requirements and metabolism of mycoplasma laidlawii. 196313973365
nucleic acid precursor requirements of mycoplasma laidlawii. 196214490761
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