Publications

sequence analysis of two cryptic plasmids from bifidobacterium longum djo10a and construction of a shuttle cloning vector.	bifidobacterium longum djo10a is a recent human isolate with probiotic characteristics and contains two plasmids, designated pdojh10l and pdojh10s. the complete sequences of both these plasmids have now been determined and consist of two circular dna molecules of 10,073 and 3,661 bp, with g+c contents of 62.2% and 66.2%, respectively. plasmid pdojh10l is a cointegrate plasmid consisting of dna regions exhibiting very high sequence identity to two other b. longum plasmids, pnac2 (98%) and pkj50 ( ...	2006	16391088
[rhodococcus equi infection].		2006	17144431
detection and characterization of bacteria from the potato rhizosphere degrading n-acyl-homoserine lactone.	quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium pectobacterium carotovorum subsp. carotovorum. the signal molecules involved in quorum sensing in p. carotovorum subsp. carotovorum belong to the group of n-acyl homoserine lactones (ahls). in our study, we screened bacteria isolated from the potato rhizosphere for the ability to degrade ahls produced by p. carotovorum subsp. carotovorum. six isolates able to degrade ahls were selected for ...	2006	17110970
molecular diversity studies of bacterial communities of oil polluted microbial mats from the etang de berre (france).	the biodiversity of microbial mats inhabiting the oil-contaminated lagoon etang de berre was determined by molecular approaches. the fingerprint of denaturing gradient gel electrophoresis (dgge) and automatic ribosomal intergenic spacer analysis (arisa) of mats exposed to different pollution levels showed specific microbial communities for each site but similar diversity richness. species composition of the mats were compared by constructing 16s rrna libraries. amplified rdna restriction analysi ...	2006	17117996
an unusual beta-vinyl effect leading to high efficiency and enantioselectivity of the amidase, nitrile biotransformations for the preparation of enantiopure 3-arylpent-4-enoic acids and amides and their applications in synthesis.	biotransformations of 3-arylpent-4-enenitriles catalyzed by rhodococcus erythropolis aj270, a nitrile hydratase/amidase-containing microbial whole-cell catalyst were studied, and an unusual beta-vinyl effect of the substrate on the biocatalytic efficiency and enantioselectivity of the amidase was observed. while 3-arylpent-4-enenitriles and 3-phenylpentanenitrile were efficiently hydrated by the action of the less r-enantioselective nitrile hydratase, the amidase showed greater activity and high ...	2006	17137391
the latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase.	in essence, evolutionary processes occur gradually, while maintaining fitness throughout. along this line, it has been proposed that the ability of a progenitor to promiscuously catalyze a low level of the evolving activity could facilitate the divergence of a new function by providing an immediate selective advantage. to directly establish a role for promiscuity in the divergence of natural enzymes, we attempted to trace the origins of a bacterial phosphotriesterase (pte), an enzyme thought to ...	2006	17105187
expression of bovine lactoferrin c-lobe in rhodococcus erythropolis and its purification and characterization.	a rhodococcus erythropolis expression system for the bovine lactoferrin c-lobe was constructed. the dna fragments encoding the blf c-lobe were amplified and cloned into vector ptip lch1.2. r. erythropolis carrying the ptip-c-lobe was cultured at 30 degrees c with shaking, and expression of the rblf c-lobe was induced by adding 1 microg/ml (final concentration) thiostrepton. the rblf c-lobe was isolated in native and denatured (8 m urea) form by ni-nta affinity chromatography. to obtain a bioacti ...	2006	17090917
chloroform degradation by butane-grown cells of rhodococcus aetherovorans bcp1.	the ability of a rhodococcus aetherovorans strain, bcp1, to grow on butane and to degrade chloroform in the 0-633 microm range (0-75.5 mg l(-1)) via aerobic cometabolism was investigated by means of resting-cell assays. bcp1 degraded chloroform with a complete mineralization of the organic cl. the resulting butane and chloroform maximum specific degradation rates were equal to 118 and 22 micromol mg(protein)(-1)day(-1), respectively. butane inhibition on chloroform degradation was satisfactorily ...	2006	17058077
heterogeneous aerobic benzene-degrading communities in oxygen-depleted groundwaters.	a sandstone aquifer beneath a petrochemicals plant (siren site, uk) is heterogeneously contaminated with benzene and oxygen-depleted. despite low redox potentials in three of the most contaminated groundwaters (benzene concentrations from 17.8 to 294 mg l(-1)), we observed aerobic benzene degradation in microcosms, indicating the presence in situ of a latent community of obligate aerobic microorganisms or an active community of facultative aerobes responding rapidly to oxygen ingress. moreover, ...	2006	17064267
vaccine-associated systemic rhodococcus erythropolis infection in farmed atlantic salmon salmo salar.	in 7 instances between 2000 and 2003, clinical investigation of populations of fresh- and seawater-reared, vaccinated, atlantic salmon salmo salar suffering total losses of between 0.1 and 35 % revealed infection with a gram-positive rod-shaped bacterium. the isolations were geographically widespread, occurring in both norway and scotland. in all cases, a gram-positive bacterium, subsequently identified as rhodococcus erythropolis, was isolated in pure culture. infections, although systemic, wer ...	2006	17067069
the ralstonia eutropha h16 phasin phap1 is targeted to intracellular triacylglycerol inclusions in rhodococcus opacus pd630 and mycobacterium smegmatis mc2155, and provides an anchor to target other proteins.	in ralstonia eutropha, the h16 phasin phap1 represents the major phasin that binds to the surface of polyhydroxyalkanoate (pha) inclusions. in this study, c-terminal fusions of phap1 with enhanced green fluorescent protein (egfp) and with escherichia coli beta-galactosidase (lacz) were expressed separately in the triacylglycerol (tag)-accumulating actinomycetes rhodococcus opacus pd630 and mycobacterium smegmatis mc(2)155, employing the m. smegmatis acetamidase (ace) promoter of the escherichia- ...	2006	17074898
pulmonary disposition of tilmicosin in foals and in vitro activity against rhodococcus equi and other common equine bacterial pathogens.	the objectives of this study were to determine the serum and pulmonary disposition of tilmicosin in foals and to investigate the in vitro activity of the drug against rhodococcus equi and other common bacterial pathogens of horses. a single dose of a new fatty acid salt formulation of tilmicosin (10 mg/kg of body weight) was administered to seven healthy 5- to 8-week-old foals by the intramuscular route. concentrations of tilmicosin were measured in serum, lung tissue, pulmonary epithelial linin ...	2006	17083461
seroepidemiological survey of rhodoccocus equi infection in asymptomatic horses from bursa, izmir and istanbul provinces, turkey.	in order to assess the rhodococcus equi infection in three provinces of turkey (bursa, izmir and istanbul), 696 sera from healthy foals and adult horses were tested by indirect elisa using a r. equi reference strain (atcc 6939) as antigen. 103 sera (14.80%) with titres >0.646 resulted positive. seroprevalence was significantly higher (p=0.0053) in male than in female horses of istanbul province, although higher antibody titres (mean value) were observed in the female group of bursa and izmir pro ...	2006	17034856
[identification of mycobacteria by sequencing of rpob gene and 16s rrna].	to classify a specific mycobacterium among various mycobacteria utilizing sequencing of rpob gene. to classify mycobacteria not identified by dna-dna hybridization (ddh) using sequencing of rpob and 16s rrna gene.	2006	17037387
the complete genome of rhodococcus sp. rha1 provides insights into a catabolic powerhouse.	rhodococcus sp. rha1 (rha1) is a potent polychlorinated biphenyl-degrading soil actinomycete that catabolizes a wide range of compounds and represents a genus of considerable industrial interest. rha1 has one of the largest bacterial genomes sequenced to date, comprising 9,702,737 bp (67% g+c) arranged in a linear chromosome and three linear plasmids. a targeted insertion methodology was developed to determine the telomeric sequences. rha1's 9,145 predicted protein-encoding genes are exceptional ...	2006	17030794
pharmacokinetics of clarithromycin and concentrations in body fluids and bronchoalveolar cells of foals.	to determine pharmacokinetics of clarithromycin and concentrations in body fluids and bronchoalveolar (bal) cells of foals.	2006	17014316
mechanism of enhanced conversion of 1,2,3-trichloropropane by mutant haloalkane dehalogenase revealed by molecular modeling.	1,2,3-trichloropropane (tcp) is a highly toxic, recalcitrant byproduct of epichlorohydrin manufacture. haloalkane dehalogenase (dhaa) from rhodococcus sp. hydrolyses the carbon-halogen bond in various halogenated compounds including tcp, but with low efficiency (k (cat)/k (m )= 36 s(-1) m(-1)). a cys176tyr-dhaa mutant with a threefold higher catalytic efficiency for tcp dehalogenation has been previously obtained by error-prone pcr. we have used molecular simulations and quantum mechanical calcu ...	2006	17016745
eukaryotic lipid body proteins in oleogenous actinomycetes and their targeting to intracellular triacylglycerol inclusions: impact on models of lipid body biogenesis.	bacterial neutral lipid inclusions are structurally related to eukaryotic lipid bodies. these lipid inclusions are composed of a matrix of triacylglycerols (tags) or wax esters surrounded by a monolayer of phospholipids. whereas the monolayers of lipid bodies from animal and plant cells harbor specific classes of proteins which are involved in the structure of the inclusions and lipid homoestasis, no such proteins are known to be associated with bacterial lipid inclusions. the present study was ...	2006	17021226
[catalysis of the biodegradation of unusable medicines by alkanotrophic rhodococci].	experiments on the biodegradation of unusable medicines containing a phenolic hydroxy group by actinobacteria of the genus rhodococcus were performed. six species and sixty-four strains were tested. it was found that rhodococci could degrade paracetamol, and some r. ruber strains showed high levels of its degradation. an efficient method for the identification and quantification of paracetamol and the products of its conversion (p-aminophenol, pyrocatechol, and hydroquinone) immediately in the c ...	2006	17022454
[improvement of the process of fluorene degradation by rhodococcus rhodochrous strain 172].	the ability of rhodococcus rhodochrous strain 172 to consume fluorene as the sole source of carbon and energy in a liquid medium was successfully increased by an addition of polycapramide fiber to the medium and preliminary adaptation of cells on fluorene agar. a combination of these approaches allowed complete degradation of fluorene without an accumulation of intermediates.	2006	17022455
alkane utilization by rhodococcus strain ntu-1 alone and in its natural association with bacillus fusiformis l-1 and ochrobactrum sp.	linear (n-hexadecane) and branched (pristane) alkanes were degraded by a mixed culture isolated from an oil-contaminated field. the degradation was accompanied by formation of biofloccules. the culture was composed of rhodococcus strain ntu-1, bacillus fusiformis l-1, and ochrobactrum sp. rhodococcus strain ntu-1 carried out the degradation of the alkane via a hydroxylase. bacillus fusiformis l-1 and ochrobactrum sp. did not degrade the alkanes but aided the flocculation by forming more rigid ba ...	2006	17022676
[cloning the amidase gene from rhodococcus rhodochrous m18 and its expression in escherichia coli].	the amidase gene from rhodococcus rhodochrous m18 was cloned by pcr amplification with primers developed by use of peptide amino acid sequences obtained after treating amidase with trypsin. nucleotide sequence analysis of this gene revealed high homology with aliphatic amidases from r. erythropolis r312 and pseudomonas aeruginosa. considering the substrate specificity and the results of dna analysis, amidase from r. rhodochrous m8 was assigned to the group of aliphatic amidases preferentially hy ...	2006	17025157
characterization of 2-bromoethanesulfonate as a selective inhibitor of the coenzyme m-dependent pathway and enzymes of bacterial aliphatic epoxide metabolism.	bacterial growth with short-chain aliphatic alkenes requires coenzyme m (com) (2-mercaptoethanesulfonic acid), which serves as the nucleophile for activation and conversion of epoxide products formed from alkene oxidation to central metabolites. in the present work the com analog 2-bromoethanesulfonate (bes) was shown to be a specific inhibitor of propylene-dependent growth of and epoxypropane metabolism by xanthobacter autotrophicus strain py2. bes (at low [millimolar] concentrations) completel ...	2006	16997966
kinetics of 1,4-dioxane biodegradation by monooxygenase-expressing bacteria.	1,4-dioxane is a probable human carcinogen, and an important emerging water contaminant. in this study, the biodegradation of dioxane by 20 bacterial isolates was evaluated, and 13 were found to be capable of transforming dioxane. dioxane served as a growth substrate for pseudonocardia dioxanivorans cb1190 and pseudonocardia benzenivorans b5, with yields of 0.09 g protein g dioxane(-1) and 0.03 g protein g dioxane(-1), respectively. cometabolic transformation of dioxane was observed for monooxyg ...	2006	16999122
association between the presence of bacterial 16s rna in prostate specimens taken during transurethral resection of prostate and subsequent risk of prostate cancer (sweden).	to study bacterial 16s rna in archival prostate samples from 352 patients with benign prostate hyperplasia (bph) and evaluate whether the presence of bacterial dna was different in those who later developed prostate cancer (n = 171) and in the matched controls that did not progress to cancer (n = 181).	2006	17006718
catabolic pathways and biotechnological applications of microbial caffeine degradation.	catabolism of caffeine (1,3,7-trimethylxanthine) in microorganisms commences via two possible mechanisms: demethylation and oxidation. through the demethylation route, the major metabolite formed in fungi is theophylline (1,3-dimethylxanthine), whereas theobromine (3,7-dimethylxanthine) is the major metabolite in bacteria. in certain bacterial species, caffeine has also been oxidized directly to trimethyl uric acid in a single step. the conversion of caffeine to its metabolites is primarily brou ...	2006	17009088
characterization of nocardia plasmid pxt107.	nocardia, rhodococcus and streptomyces, all members of the actinomycetes family, are gram-positive eubacteria with high g+c content and able to form mycelium. we report here a newly identified plasmid pxt107 of nocardia sp. 107, one of the smallest circular plasmids found in nocardia. the complete nucleotide sequence of pxt107 consisted of 4335 bp with 65% g+c content, and encoded one replication extragenic palindromic (rep) and six hypothetical proteins. the rep, double-strand origin and single ...	2006	16953300
variations in equid slc11a1 (nramp1) genes and associations with rhodococcus equi pneumonia in horses.	rhodococcus equi is an important intracellular pathogen of horses, most commonly causing chronic, suppurative bronchopneumonia in foals. although most foals likely are exposed to environmental r. equi within the 1st few days of life, only some develop r. equi pneumonia, and the basis of differences in susceptibility among foals currently is unknown. in this study, we investigated solute carrier family 11 member 1 (slc11a1) gene sequences in the 5' untranslated region, exon 1, and a portion of in ...	2006	16955825
associations between the ecology of virulent rhodococcus equi and the epidemiology of r. equi pneumonia on australian thoroughbred farms.	the ecology of virulent strains of rhodococcus equi on horse farms is likely to influence the prevalence and severity of r. equi pneumonia in foals. this study examined the association between the ecology of virulent r. equi and the epidemiology of r. equi pneumonia by collecting air and soil samples over two breeding seasons (28 farm-year combinations) on thoroughbred breeding farms with different reported prevalences of r. equi pneumonia. colony blotting and dna hybridization were used to dete ...	2006	16957241
transcriptomic assessment of isozymes in the biphenyl pathway of rhodococcus sp. strain rha1.	rhodococcus sp. rha1 grows on a broad range of aromatic compounds and vigorously degrades polychlorinated biphenyls (pcbs). previous work identified rha1 genes encoding multiple isozymes for most of the seven steps of the biphenyl (bph) pathway, provided evidence for coexpression of some of these isozymes, and indicated the involvement of some of these enzymes in the degradation of bph, ethylbenzene (etb), and pcbs. to investigate the expression of these isozymes and better understand how they c ...	2006	16957245
microbial population dynamics associated with crude-oil biodegradation in diverse soils.	soil bacterial population dynamics were examined in several crude-oil-contaminated soils to identify those organisms associated with alkane degradation and to assess patterns in microbial response across disparate soils. seven soil types obtained from six geographically distinct areas of the united states (arizona, oregon, indiana, virginia, oklahoma, and montana) were used in controlled contamination experiments containing 2% (wt/wt) crude oil spiked with [1-(14)c]hexadecane. microbial populati ...	2006	16957258
a square archaeon, the smallest eukaryote and the largest bacteria.		2006	16958749
a novel nadp+-dependent l-1-amino-2-propanol dehydrogenase from rhodococcus erythropolis mak154: a promising enzyme for the production of double chiral aminoalcohols.	a novel nadp(+)-dependent l-1-amino-2-propanol dehydrogenase was isolated from rhodococcus erythropolis mak154, and characterized.	2006	16965375
intracellular success: cytologic findings in an ulcerated submandibular mass from a cat.	a 1-year-old neutered male domestic shorthair cat had an ulcerated, proliferative lesion in the submandibular area that did not respond to antibiotic therapy. impression smears from the mass revealed septic pyogranulomatous inflammation, with large numbers of pleomorphic bacteria observed intracellularly within macrophages as well as neutrophils. bacterial culture was consistent with a diagnosis of rhodococcus equi, a facultative intracellular coccobacillus capable of replicating within macropha ...	2006	16967424
functional expression of the particulate methane mono-oxygenase gene in recombinant rhodococcus erythropolis.	in order to construct an expression system for the particulate methane mono-oxygenase (pmmo) gene (pmo), the structural gene cluster pmocab amplified from methylosinus trichosporium ob3b was inserted into a shuttle vector pbs305 under the control of a dsz promoter and transformed into rhodococcus erythropolis lsse8-1. a stable transformant was successfully obtained using ethane as the sole carbon source. fluorescence in situ hybridization results showed that the dsz promoter allowed the pmo gene ...	2006	16978347
cholesterol oxidase (choe) is not important in the virulence of rhodococcus equi.	to analyze further the role in virulence of the prominent cholesterol oxidase (choe) of rhodococcus equi, an allelic exchange choe mutant from strain 103+ was constructed and assessed for virulence in macrophages, in mice, and in foals. there was no difference between the mutant and parent strain in cytotoxic activity for macrophages or in intra-macrophage multiplication. no evidence of attenuation was obtained in macrophages and in mice, but there was slight attenuation apparent in four intra-b ...	2006	16979852
the surfactant tween 80 enhances biodesulfurization.	in biocatalytic conversions, substrates and products may display inhibitory or toxic effects on the biocatalyst. rhodococcus erythropolis 1awq could further remove sulfur from hydrodesulfurized diesel oil, and the biodesulfurization was enhanced by the surfactant tween 80. tween 80 was shown to decrease the product concentration associated with the cells, reducing product inhibition.	2006	16980422
[diagnosis and therapy of rhodococcus equi infection in the horse].	infection with rhodococcus equi is an important cause of pneumonia in foals, but other organ systems may also be affected. the intracellular presence of r. equi and the formation of granulomatous and suppurative inflammatory tissue mean that prolonged treatment is needed. the pharmacological properties of the combination of erythromycin and rifampicin have improved the survival of foals infected with r. equi; however, erythromycin can cause adverse reactions in foals and mares, which has prompte ...	2006	16989420
[vertebral osteomyelitis caused by rhodococcus equi in a three-and-half-month-old dutch warmblood foal].	rhodococcus equi infection occurs worldwide and is especially a problem in foals, where it often causes colitis or pneumonia. other organs are seldom affected, and their involvement is regarded as a complication of pneumonia and/or colitis. vertebral osteomyelitis is one such rare complication and is probably caused by haematogenous spread from inflammatory lesions in the lungs and/or intestine. in rare cases, osteomyelitis can be caused by contamination of a wound. this case study describes a f ...	2006	16989421
etiological misidentification by routine biochemical tests of bacteremia caused by gordonia terrae infection in the course of an episode of acute cholecystitis.	gordonia terrae has been reported to be a rare cause of bacteremia. we report the first case of bacteremia associated with acute cholecystitis. commercial biochemical testing was not able to identify the strain at the genus level, classifying it instead as rhodococcus sp. definitive identification was obtained by sequencing of the 16s rrna gene.	2006	16825404
biodegradation by members of the genus rhodococcus: biochemistry, physiology, and genetic adaptation.		2006	16829254
rhodococcus sp. strain tm1 plays a synergistic role in the degradation of piperidine by mycobacterium sp. strain tho100.	mycobacterium sp. strain tho100 and rhodococcus sp. strain tm1 were isolated from a morpholine-containing enrichment culture of activated sewage sludge. strain tho100, but not strain tm1, was able to degrade alicyclic amines such as morpholine, piperidine, and pyrrolidine. the mixed strains tho100 and tm1 showed a better growth on piperidine as the substrate than the pure strain tho100 because strain tm1 was able to reduce the level of glutaraldehyde (ga) produced during piperidine degradation. ...	2006	16832627
[pneumonia caused rhodococcus equi in a immunocompromised patient treated with corticosteroids].		2006	16834958
dramatic enhancement of enantioselectivity of biotransformations of beta-hydroxy nitriles using a simple o-benzyl protection/docking group.	[structure: see text] catalyzed by the rhodococcus erythropolis aj270 whole cell catalyst, the o-benzylated beta-hydroxy alkanenitriles underwent remarkably high enantioselective biotransformations, whereas the biotransformations of free beta-hydroxy alkanenitriles gave very low enantioselectivity. the easy manipulations of o-protection and o-deprotection, excellent chemical and enantiomeric yields of biotransformations, along with the scalability render this enzymatic transformation attractive ...	2006	16836373
replication and inheritance of nocardia plasmid pc1.	nocardia sp. c-14-1, isolated from acrylic fiber wastewater, can degrade long-chain alkanes and succinonitrile efficiently. here we report the characterization of an indigenous plasmid pc1. the overall nucleotide sequence of pc1 consisted of 5841 bp. the five orfs, encoding a dna recombinase, replication protein (rep(pc1)) and three proteins of unknown function, were predicted on pc1. the rep(pc1) displayed its homology with the rep of rhodococcus large plasmid p33701, suggesting a theta type of ...	2006	16842357
n-acylhomoserine lactonase producing rhodococcus spp. with different ahl-degrading activities.	n-acylhomoserine lactones (ahls) are conserved signal molecules that control diverse biological activities in quorum sensing system of gram-negative bacteria. recently, several soil bacteria were found to degrade ahls, thereby interfering with the quorum sensing system. previously, rhodococcus erythropolis w2 was reported to degrade ahls by both oxido-reductase and ahl-acylase. in the present study, two ahl-utilizing bacteria, strains ls31 and pi33, were isolated and identified as the genus rhod ...	2006	16842366
proteasome assembly triggers a switch required for active-site maturation.	the processing of propeptides and the maturation of 20s proteasomes require the association of beta rings from two half proteasomes. we propose an assembly-dependent activation model in which interactions between helix (h3 and h4) residues of the opposing half proteasomes are prerequisite for appropriate positioning of the s2-s3 loop; such positioning enables correct coordination of the active-site residue needed for propeptide cleavage. mutations of h3 or h4 residues that participate in the ass ...	2006	16843899
detection, isolation and partial characterization of an immunostimulating glycoprotein from rhodococcus fascians.	in a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, rhodococcus fascians, were able to induce cytokine release (tnf(alpha)) from mouse peritoneal macrophages. monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (mw>100 kda) glycoprotein. in addition, methods practicable for its biotechnological preparation and sev ...	2006	16846838
modulation of eukaryotic cell apoptosis by members of the bacterial order actinomycetales.	apoptosis, or programmed cell death, is normally responsible for the orderly elimination of aged or damaged cells, and is a necessary part of the homeostasis and development of multicellular organisms. some pathogenic bacteria can disrupt this process by triggering excess apoptosis or by preventing it when appropriate. either event can lead to disease. there has been extensive research into the modulation of host cell death by microorganisms, and several reviews have been published on the phenom ...	2006	16850163
vapi, a new member of the rhodococcus equi vap family.	rhodococcus equi is a facultative intracellular bacterium which can cause bronchopneumonia in foals and aids patients. in this report we show that the orf13-protein coded by the virulence associated plasmid of r. equi is clearly homologous to vape. nucleotide sequence analysis revealed frame shift mutations that shorten the sequence of the orf13-protein. a theoretical extension of the sequence of orf13 by the introduction of a single nucleotide yields a translated amino acid sequence that is hig ...	2006	16871422
culturable actinobacteria from the marine sponge hymeniacidon perleve: isolation and phylogenetic diversity by 16s rrna gene-rflp analysis.	a total of 106 actinobacteria associated with the marine sponge hymeniacidon perleve collected from the yellow sea, china were isolated using eight different media. the number of species and genera of actinobacteria recovered from the different media varied significantly, underlining the importance of optimizing the isolation conditions. the phylogenetic diversity of the actinobacteria isolates was assessed using 16s rrna gene amplification-restriction fragment length polymorphism (rflp) analysi ...	2006	16871424
the isolation of microorganisms capable of phenol degradation.	the results of a study on the composition of microflora settling the pilot biofilter bed that purifies the exhausting gases from a cable factory's coil-wire varnishing division are presented in this study. the ability of isolated bacterial strains to biodegrade phenol was also evaluated using culture media of various compositions. phenol was introduced into the medium at the following concentrations: 0.25, 0.5, 0.75 and 1 g x dm(-3). in addition, air in desiccators, where micro-organisms grew, w ...	2006	16878606
multiple-subunit genes of the aromatic-ring-hydroxylating dioxygenase play an active role in biphenyl and polychlorinated biphenyl degradation in rhodococcus sp. strain rha1.	a gram-positive strong polychlorinated biphenyl (pcb) degrader, rhodococcus sp. strain rha1, can degrade pcbs by cometabolism with biphenyl or ethylbenzene. in rha1, three sets of aromatic-ring-hydroxylating dioxygenase genes are induced by biphenyl. the large and small subunits of their terminal dioxygenase components are encoded by bpha1 and bpha2, etba1 and etba2, and ebda1 and ebda2, respectively, and the deduced amino acid sequences of etba1 and etba2 are identical to those of ebda1 and ebd ...	2006	16885291
crystal structure and desulfurization mechanism of 2'-hydroxybiphenyl-2-sulfinic acid desulfinase.	the desulfurization of dibenzothiophene in rhodococcus erythropolis is catalyzed by two monooxygenases, dsza and dszc, and a desulfinase, dszb. in the last step of this pathway, dszb hydrolyzes 2'-hydroxybiphenyl-2-sulfinic acid into 2-hydroxybiphenyl and sulfite. we report on the crystal structures of dszb and an inactive mutant of dszb in complex with substrates at resolutions of 1.8a or better. the overall fold of dszb is similar to those of periplasmic substrate-binding proteins. in the subs ...	2006	16891315
rhodococcus equi in the soil environment of horses in inner mongolia, china.	little is known about the distribution of rhodococcus equi in the soil environment of native horses in china. one hundred and eight soil samples were collected from native-horse farms in the hulun beier grasslands of eastern mongolia, the xilin goler grasslands of southern mongolia, and tongliao city in inner mongolia, china. the isolation rates of r. equi from soil samples from the hulun beier and xilin goler grasslands ranged from 25.9% to 30.0%. in contrast, isolation rates from soil samples ...	2006	16891789
nitrile hydrolysis activity of rhodococcus erythropolis ncimb 11540 whole cells.	the nitrile hydrolyzing properties of the bacterium strain rhodococcus erythropolis ncimb 11540 have been investigated. using whole cells of the microorganism, a wide variety of aromatic and aliphatic cyanide-containing substrates was successfully hydrolyzed to the corresponding amide or acid. in the case of dicyanides, selective monohydrolysis took place, which was further explored in the desymmetrization of malononitriles resulting in the corresponding cyano amides in enantiomeric excesses of ...	2006	16892293
variations of bacterial community structure in flooded paddy soil contaminated with herbicide quinclorac.	the denaturing gradient gel electrophoresis (dgge) method was applied to determine the relative genetic complexity of microbial communities in flooded paddy soil treated with herbicide quinclorac (3,7-dichloro-8-quinoline-carboylic acid). the results obtained showed a significant effect of quinclorac on the development of bacterial populations in soils contaminated with different concentrations of the herbicide at the early time after application. in general, however, the number of populations o ...	2006	16893772
rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete.	a gram-positive actinobacterium, strain rkj300(t), capable of utilizing p-nitrophenol and 2,4-dinitrophenol, was isolated from a pesticide-contaminated site in india. the morphological and chemotaxonomic properties of the isolate were typical of members of the genus rhodococcus. the dna g+c content was 72 mol%. strain rkj300(t) exhibited the highest level of sequence similarity with rhodococcus wratislaviensis ncimb 13082(t) (99.3 %), followed by rhodococcus opacus dsm 43205(t) (98.8 %), rhodoco ...	2006	16902038
biosurfactant production by rhodococcus erythropolis grown on glycerol as sole carbon source.	the production of biosurfactant by rhodococcus erythropolis during the growth on glycerol was investigated. the process was carried out at 28 degrees c in a 1.5-l bioreactor using glycerol as carbon source. the bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. after 51 h of cultivation, 1.7 g/l of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mn/m, respectively, 67% of emulsifying index (e24), and 94% ...	2006	16915696
[performance of demulsification by rhodococcus sp. pr-1].	rhodococcus sp. pr-1 with high capability of demulsification on a surfactant-stabilized kerosene-water model emulsion was isolated from sewage of dagang oil field. it could demulsify the model emulsion completely in 8 hours at 55 degrees c and had better demulsifying capability than chemical demulsifier dgf-01. the freezing-thawing and autoclaving had no effect on the process of demulsification, yet ultrasonic disposal and deal with organic solvent could inhibit its activity. a linear relationsh ...	2006	16921960
genetic diversity of dioxygenase genes in polycyclic aromatic hydrocarbon-degrading bacteria isolated from mangrove sediments.	to investigate the diversity of dioxygenase genes involved in polycyclic aromatic hydrocarbon (pah)-degradation, a total of 32 bacterial strains were isolated from surface mangrove sediments, from the genera mycobacterium, sphingomonas, terrabacter, sphingopyxis, sphingobium and rhodococcus. two sets of pcr primers were constructed to detect the nida-like and nahac-like sequences of the alpha subunit of the pah ring-hydroxylating dioxygenase. pcr amplified the dna fragments from all gram-positiv ...	2006	16923069
protocol for mutagenesis of alkene monooxygenase and screening for modified enantiocomposition of the epoxypropane product.	alkene monooxygenase (amo) from rhodococcus rhodochrous b-276 is a 3-component enzyme system encoded by the 4-gene operon amoabcd, which catalyzes the stereoselective epoxidation of aliphatic alkenes yielding primarily the r enantiomer. with propene as the substrate, wild-type amo yields r-epoxypropane with an enantiomeric excess (e.e.) of 83%. the presumed site of alkene oxidation is a dinuclear iron center situated within the large subunit of the epoxygenase component, amoc. substantial proble ...	2006	16928986
[long term survival after rhodococcus equi pneumonia in a patient with human immunodeficiency virus infection in the era of highly active antiretroviral therapy: case report and review].	before highly active antiretroviral therapy (haart) has become available, antibiotic treatment was usually unable to eradicate rhodococcus (r.) equi infection in hiv-infected patients, although some clinical improvement could be observed in most cases. there are limited data on the outcome of treatment of r. equi pneumonia in the haart era. we report on a 52-year-old hiv-infected man who presented in poor general condition with an extensive lung cavitation lesion caused by r. equi. the patient r ...	2006	16933840
simultaneous biodetoxification of s, n, and o pollutants by engineering of a carbazole-degrading gene cassette in a recombinant biocatalyst.	the gene cassette encoding enzymes responsible for degrading carbazole to anthranilic acid was introduced into a dibenzothiophene degrader. the resultant strain, rhodococcus erythropolis xpdn, could simultaneously transform the model pollutants dibenzothiophene, carbazole, and dibenzofuran to nontoxic metabolites and may have an application potential for bioremediation.	2006	16936043
[evaluation of api coryne system, version 2.0, for diphteroid gram-positive rods identification with clinical relevance].	the ability of the api coryne system, version 2.0, to identify 178 strains of gram-positive rods was evaluated. seventy eight isolates belonged to genus corynebacterium and one hundred to related genera, all strains were isolated from clinical samples at the laboratory of bacteriology, hospital de clínicas josé de san martin (uba) between 1995 and 2004. the isolates were identified according to von graevenitz and funke's scheme. one hundred and sixty two out of 178 strains (91%) were correctly i ...	2006	17370571
rhodococcus equi infection in acquired immunodeficiency syndrome. computed tomography aspects.	to present the computed tomography aspects of rhodococcus equi pneumonia in seven patients with acquired immunodeficiency syndrome.	2006	17268743
survey of the large-animal diplomates of the american college of veterinary internal medicine regarding knowledge and clinical use of polymerase chain reaction: implications for veterinary education.	a questionnaire was developed to document the knowledge base of large-animal diplomates of the american college of veterinary internal medicine (acvim) regarding polymerase chain reaction (pcr) technology and to identify the common use of this technology in equine practice. ninety-three of the 278 mailed questionnaires were returned, for an overall response rate of 33.4%. ninety respondents (99%) reported being familiar with the general principles of nucleic acid probe technology; however, only ...	2006	17220506
specificity of catechol ortho-cleavage during para-toluate degradation by rhodococcus opacus 1cp.	degradation of para-toluate by rhodococcus opacus 1cp was investigated. activities of the key enzymes of this process, catechol 1,2-dioxygenase and muconate cycloisomerase, are detected in this microorganism. growth on p-toluate was accompanied by induction of two catechol 1,2-dioxygenases. the substrate specificity and physicochemical properties of one enzyme are identical to those of chlorocatechol 1,2-dioxygenase; induction of the latter enzyme was observed during r. opacus 1cp growth on 4-ch ...	2006	17223783
effect of hyperimmune plasma on the severity of pneumonia caused by rhodococcus equi in experimentally infected foals.	this study evaluated the prophylactic effectiveness of hyperimmune plasma (hip) as an aid in the prevention of pneumonia caused by experimental infection with rhodococcus equi. thirty neonatal foals were administered r. equi hip or saline at 2 days of age and were infected with virulent r. equi at 7 days. all foals developed signs or symptoms of respiratory disease. radiographic scores on day 28 and neutrophil concentrations on day 49 were significantly greater in control foals, and time to resp ...	2006	17216591
chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (capsicum anuum l.).	a climate chamber experiment was conducted to assay the effect of low temperatures (chilling) on the diversity of bacteria colonizing the endospheres of two thermophilic sweet pepper (capsicum anuum l.) cultivars, milder spiral and ziegenhorn bello. structural diversity was analyzed by 16s rrna-based terminal restriction fragment length polymorphism (t-rflp) analysis and by the generation of 16s rrna gene libraries to determine dominant community members in t-rflp profiles. cultivable community ...	2006	17215894
[bacteremia due to rhodococcus equi in a patient with acquired immunodeficiency syndrome: case report].	rhodococcus equi is an important agent for zoonotic infections, and may cause serious infections in humans, especially immunocompromised patients. in this study, a case of fatal bacteremia due to rhodococcus equi in a patient with acquired immunodeficiency syndrome (hiv positive) is reported.	2006	17308706
isolation and characterization of gasoline-degrading bacteria from gas station leaking-contaminated soils.	the effects of culture conditions in vitro and biosurfactant detection were studied on bacterial strains capable of degrading gasoline from contaminated soils near gas station. the main results were summarized as follows. three bacteria (strains q 10, q14 and q18) that were considered as efficiently degrading strains were isolated and identified as pseudomonas sp., flavobacterium sp. and rhodococcus sp., respectively. the optimal growth conditions of three bacteria including ph, temperature and ...	2006	17278756
probing the substrate specificity of the catalytically self-sufficient cytochrome p450 rhf from a rhodococcus sp.	analysis of the substrate specificity of the self-sufficient cytochrome p450 rhf revealed that the enzyme tends to catalyse the dealkylation of substituted alkyl-aryl ethers with shorter alkyl moieties more readily than equivalent compounds with longer alkyl groups.	2006	17283795
cerebellar abscess due to rhodococcus equi in an immunocompetent patient: case report and literature review.	rhodococcus equi (r. equi) is a facultative, intracellular, nonmotile, non-spore-forming, gram-positive coccobacillus, primarily causes zoonotic infections that affect grazing animals, mainly horses and foals and is a rare opportunistic pathogen found in severely compromised patients, and most commonly in recent years, in human immunodeficiency virus (hiv)-infected persons. early cases, most in patients receiving immunosuppressant therapy, were more likely to be successfully treated with antimic ...	2006	17285106
the impact of acrylonitrile and bioaugmentation on the biodegradation activity and bacterial community structure of a topsoil.	the analysis of the bacterial community within the soil using dgge showed acrylonitrile (acn) could lead to the selection of significantly similar communities. moreover, rhodococcus sp. aj270 was successfully established in the soil community. high gc g+-bacteria also responded positively to acn addition. bioaugmentation or carbon addition had no impact on the rate or degree of acn degradation. acn could be readily degraded by the soil bacteria, however, the community structure was significantly ...	2006	17455796
endosulfan degradation by a rhodococcus strain isolated from earthworm gut.	a rhodococcus mtcc 6716 bacterial strain was isolated apparently for the first time from the gut microflora of an indian earthworm (metaphire posthuma). endosulfan was used as a carbon source by the strain and degraded it up to 92.58% within 15 days. furthermore, the isolated strain of the bacterium did not produce the persistent form of the toxic metabolite endosulfan sulfate. this strain exhibits luxury growth in minimal medium with high concentrations of endosulfan (80 microg ml(-1)). degrada ...	2006	16029891
genetic diversity of culturable bacteria in oil-contaminated rhizosphere of galega orientalis.	a collection of 50 indigenous meta-toluate tolerating bacteria isolated from oil-contaminated rhizosphere of galega orientalis on selective medium was characterized and identified by classical and molecular methods. 16s rdna partial sequencing showed the presence of five major lineages of the bacteria domain. gram-positive rhodococcus, bacillus and arthrobacter and gram-negative pseudomonas were the most abundant genera. only one-fifth of the strains that tolerated m-toluate also degraded m-tolu ...	2006	16055251
construction of random transposition mutagenesis system in rhodococcuserythropolis using is1415.	recent studies on the metabolic activities of genus rhodococcus have shown rhodococci to be of important use in industrial, pharmaceutical and environmental biotechnology. the increasing economic significance of rhodococcus encourages renewed efforts to characterize their genetic systems, as rhodococcus genetics are still poorly understood. the goal of this study is to adapt a transposon system for use in creating random mutagenesis in rhodococcus erythropolis. a plasmid carrying is1415, a membe ...	2006	16107286
metabolic engineering for synthesis of aryl carotenoids in rhodococcus.	rhodococcus erythropolis naturally synthesizes monocyclic carotenoids: 4-keto-gamma-carotene and gamma-carotene. the genes and the pathway for carotenoid synthesis in r. erythropolis were previously described. we heterologously expressed a beta-carotene desaturase gene (crtu) from brevibacterium in rhodococcus to produce aryl carotenoids such as chlorobactene. expression of the crtu downstream of a chloramphenicol resistance gene on prhbr171 vector showed higher activity than expression downstre ...	2006	16133327
identification and structural characterisation of novel trehalose dinocardiomycolates from n-alkane-grown rhodococcus opacus 1cp.	rhodococcus opacus 1cp, a potent degrader of (chloro-) aromatic compounds was found to utilise c10-c16 n-alkanes as sole carbon sources. highest conversion rates were observed with n-tetradecane and n-hexadecane, whereas the utilisation of n-dodecane and n-decane was considerably slower. thin-layer chromatography of organic extracts of n-alkane-grown 1cp cultures indicated the growth-associated formation of a glycolipid which was characterised as a trehalose dimycolate by 1h-nmr spectroscopy and ...	2006	16133336
detection of equine herpesvirus type 1 using a real-time polymerase chain reaction.	equid herpesvirus 1 (ehv1) is a major disease of equids worldwide causing considerable losses to the horse industry. a variety of techniques, including pcr have been used to diagnose ehv1. some of these pcrs were used in combination with other techniques such as restriction enzyme analysis (rea) or hybridisation, making them cumbersome for routine diagnostic testing and increasing the chances of cross-contamination. furthermore, they involve the use of suspected carcinogens such as ethidium brom ...	2006	16137772
constitutive expression of catabc genes in the aniline-assimilating bacterium rhodococcus species an-22: production, purification, characterization and gene analysis of cata, catb and catc.	the aniline-assimilating bacterium rhodococcus sp. an-22 was found to constitutively synthesize catb (cis,cis-muconate cycloisomerase) and catc (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported cata (catechol 1,2-dioxygenase). the bacterium maintained the specific activity of the three enzymes at an almost equal level during cultivation on succinate. catb and catc were purified to homogeneity and characterized. catb was a monomer wi ...	2006	16156722
isolation of a bacterium that degrades urethane compounds and characterization of its urethane hydrolase.	a bacterium which degrades urethane compounds was isolated and identified as rhodococcus equi strain tb-60. strain tb-60 degraded toluene-2,4-dicarbamic acid dibutyl ester (tdcb) and accumulated toluene diamine as the degradation product. the enzyme which cleaves urethane bond in tdcb was strongly induced by acetanilide. the purified enzyme (urethane hydrolase) was found to be homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the molecular weight was estimated to be 55 kd ...	2006	16041575
rhodococcus sp. f92 immobilized on polyurethane foam shows ability to degrade various petroleum products.	this work reports on the immobilization and performance of a hydrocarbon-degrading microorganism on polyurethane foam (puf) in the bioremediation of petroleum hydrocarbons. the ability of four different microorganisms to immobilize on puf and to degrade various petroleum products (arabian light crude (alc), al-shaheen crude (asc), diesel and oil slops) was assessed by measuring the n-alkane fraction remaining in the petroleum products over time. a rhodococcus sp. (designated as f92) had the high ...	2006	16154500
functional expression system for cytochrome p450 genes using the reductase domain of self-sufficient p450rhf from rhodococcus sp. ncimb 9784.	cytochrome p450rhf from rhodococcus sp. ncimb 9784 is a self-sufficient p450 monooxygenase. we report here a simple system for the functional expression of various p450 genes using the reductase domain of this p450rhf, which comprises flavin mononucleotide- and nicotinamide adenine dinucleotide phosphate binding motifs and a [2fe2s] ferredoxin-like center. vector pred was constructed, which carried the t7 promoter, cloning sites for a p450, a linker sequence, and the p450rhf reductase domain, in ...	2006	16195793
characterization of gordonia sp. strain f.5.25.8 capable of dibenzothiophene desulfurization and carbazole utilization.	a dibenzothiophene (dbt)-degrading bacterial strain able to utilize carbazole as the only source of nitrogen was identified as gordonia sp. f.5.25.8 due to its 16s rrna gene sequence and phenotypic characteristics. gas chromatography (gc) and gc-mass spectroscopy analyses showed that strain f.5.25.8 transformed dbt into 2-hydroxybiphenyl (2-hbp). this strain was also able to grow using various organic sulfur or nitrogen compounds as the sole sulfur or nitrogen sources. resting-cell studies indic ...	2006	16211383
a lava rock-based biofilter for the treatment of alpha-pinene.	biofiltration is an emerging technology in the united states that utilizes microorganisms to biodegrade harmful contaminants in air to carbon dioxide and water. biofiltration is not only more cost effective, but also more environmentally friendly than traditional technologies such as thermal oxidation and chemical scrubbing. the primary objectives of the study were to operate a lava rock-based laboratory biofiltration system for the removal of alpha-pinene. a consortium of microorganisms to be u ...	2006	16213705
continuous production of l(+)-tartaric acid from cis-epoxysuccinate using a membrane recycle reactor.	the one-step bioconversion of cis-epoxysuccinate (ces) to l(+)-tartaric acid by dried rhodococcus rhodochrous cells containing ces hydrolase activity was studied by using a continuous bioconversion process. the influence of the ph and the temperature was assessed. a mathematical model was used to quantify the ces hydrolase activity and stability. the optimal ph, which resulted in a maximal ces hydrolase activity and stability, was ph 8.0. a large increase in stability (half-life time) could be o ...	2006	16217652
[pcb degradation systems in rhodococcus bacteria: multiplex enzyme system determined by multiple isozyme genes].		2005	16218454
catalytic mechanism of limonene epoxide hydrolase, a theoretical study.	the catalytic mechanism of limonene epoxide hydrolase (leh) was investigated theoretically using the density functional theory method b3lyp. leh is part of a novel limonene degradation pathway found in rhodococcus erythropolis dcl14, where it catalyzes the hydrolysis of limonene-1,2-epoxide to give limonene-1,2-diol. the recent crystal structure of leh was used to build a model of the leh active site composed of five amino acids and a crystallographically observed water molecule. with this model ...	2005	16218628
recognition of a b-cell epitope of the vapa protein of rhodococcus equi in newborn and experimentally infected foals.	the aim of this study was to evaluate the usefulness of the previously identified b-cell epitope tslnlqkdepngrasdtagq of the vapa protein of rhodococcus equi and its association with r. equi pneumonia. a modified peptide designated pn11-14 corresponding to the epitope was recognized by all sera from experimentally infected foals with virulent r. equi atcc103+ containing the virulence plasmid but not by its plasmid-cured derivative atcc103- strain. marked levels of vapa-specific immunoglobulin (i ...	2005	16219093
[the role of intercellular contacts in the initiation of growth and in the development of a transiently nonculturable state by the cultures of rhodococcus rhodochrous grown in poor media].	it was found that the growth of rhodococcus rhodochrous cells in modified saton's medium strongly depends on the rate of culture agitation in the flask: an agitation at 250 rpm in flasks with baffles stops cell multiplication, whereas slight agitation leads to pronounced culture growth. the growth retardation phenomenon was reversible and did not manifest itself in exponential-phase cultures or when the cells were grown in a rich medium; furthermore, it was not connected with the degree of cultu ...	2005	16211852
[monitoring of microbial degraders in manned space stations].	samples of microorganisms from the surface of constructions of mir space station (mir ss) were taken and examined after 13 years of operation. the following microorganisms were isolated and identified: 12 fungal species belonging to the genera penicillium, aspergillus, cladosporium, and aureobasidium; 3 yeast species belonging to the genera debaryomyces, candida, and rhodotorula; and 4 bacterial species belonging to the genera bacillus, myxococcus, and rhodococcus. the predominant species in all ...	2005	16212041
n-acylhomoserine lactone quorum-sensing molecules are modified and degraded by rhodococcus erythropolis w2 by both amidolytic and novel oxidoreductase activities.	the rhodococcus erythropolis strain w2 has been shown previously to degrade the n-acylhomoserine lactone (ahl) quorum-sensing signal molecule n-hexanoyl-l-homoserine lactone, produced by other bacteria. data presented here indicate that this gram-positive bacterium is also capable of using various ahls as the sole carbon and energy source. the enzymic activities responsible for ahl inactivation were investigated in r. erythropolis cell extracts and in whole cells. r. erythropolis cells rapidly d ...	2005	16207914
fatal pulmonary infection associated with a novel organism, "para-streptomyces abscessus".	actinomycetes are increasingly recognized as pathogenic in the immunocompromised host. we isolated an asporogenous, nonmotile, aerobic gram-positive rod from a transplant recipient with a fatal pulmonary infection. the pathology was similar to that associated with rhodococcus equi, including intra-histiocytic localization. the organism was relatively inert in standard biochemical tests. 16s rrna gene sequencing indicated a potentially unique organism most closely related to the genus streptomyce ...	2005	16208023
characteristics of styrene degradation by rhodococcus pyridinovorans isolated from a biofilter.	a novel strain (pyj-1) of rhodococcus pyridinovorans that was isolated from a biofilter was able to degrade styrene at a maximum rate of 0.16 mg (mg protein)(-1) h(-1) in batch culture at 97 mg l(-1) of initial styrene gas concentration. the optimum ph and temperature for styrene degradation were 7 and 32 degrees c, respectively. the degradation kinetic constants were obtained using substrate inhibition kinetics. in a perlite-packed biofilter the maximum styrene removal rate by the strain was 27 ...	2005	16202798
role of the ssu and seu genes of corynebacterium glutamicum atcc 13032 in utilization of sulfonates and sulfonate esters as sulfur sources.	corynebacterium glutamicum atcc 13032 was found to be able to utilize a broad range of sulfonates and sulfonate esters as sulfur sources. the two gene clusters potentially involved in sulfonate utilization, ssud1cba and ssui-seuabc-ssud2, were identified in the genome of c. glutamicum atcc 13032 by similarity searches. while the ssu genes encode proteins resembling ssu proteins from escherichia coli or bacillus subtilis, the seu gene products exhibited similarity to the dibenzothiophene-degradin ...	2005	16204527
comparison of selective media for the isolation of rhodococcus equi and description of a new selective plating medium.	in order to improve the isolation rate of rhodococcus equi from animals and soil, the efficacy of four previously described selective media (caz-nb, m3t, nanat and tinsdale) and that of four other media (nc, pnp, tcp and tvp) composed by us was compared and evaluated. two selective plating media proved to be the best for the isolation of r. equi from contaminated samples. one of them was caz-nb containing ceftazidime, novobiocin and cycloheximide, while the other was the newly composed tcp conta ...	2005	16156123
structure of an atypical epoxide hydrolase from mycobacterium tuberculosis gives insights into its function.	epoxide hydrolases are vital to many organisms by virtue of their roles in detoxification, metabolism and processing of signaling molecules. the mycobacterium tuberculosis genome encodes an unusually large number of epoxide hydrolases, suggesting that they might be of particular importance to these bacteria. we report here the first structure of an epoxide hydrolase from m.tuberculosis, solved to a resolution of 2.5 a using single-wavelength anomalous dispersion (sad) from a selenomethionine-sub ...	2005	16051262
the signature amidase from sulfolobus solfataricus belongs to the cx3c subgroup of enzymes cleaving both amides and nitriles. ser195 and cys145 are predicted to be the active site nucleophiles.	the signature amidase from the extremophile archeum sulfolobus solfataricus is an enantioselective enzyme that cleaves s-amides. we report here that this enzyme also converts nitriles in the corresponding organic acid, similarly to the well characterized amidase from rhodococcus rhodochrous j1. the archaeal and rhodococcal enzymes belong to the signature amidases and contain the typical serine-glycine rich motif. they work at different optimal temperature, share a high sequence similarity and bo ...	2005	16156792