Publications

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epizootiological characteristics of viable bacteria and fungi in indoor air from porcine, chicken, or bovine husbandry confinement buildings.microorganisms found in bioaerosols from animal confinement buildings not only foster the risk of spreading diseases among livestock buildings, but also pose health hazards to farm workers and nearby residents. this study identified the various microorganisms present in the air of swine, chicken, and cattle farms with different kinds of ventilation conditions in korea. microbial air samples were collected onto petri dishes with bacterial or fungal growth media using a cascade impactor. endotoxin ...201627456779
comparative evaluation of antimicrobial activity of qmix, 2.5% sodium hypochlorite, 2% chlorhexidine, guava leaf extract and aloevera extract against enterococcus faecalis and candida albicans - an in-vitro study.debridement and disinfection of the root canal system is a critical step in endodontic treatment. most of the irrigants presently used in the endodontic treatment can have an impact on the microbes surviving in the biofilm but none of them are able to do all of the required tasks. researches are going on its full swing in order to produce an endodontic irrigant having ideal properties.201627437354
novel benzimidazol-2-ylidene carbene precursors and their silver(i) complexes: potential antimicrobial agents.novel benzimidazolium salts were synthesized as n-heterocyclic carbene (nhc) precursors, these nhc precursors were metallated with ag2o in dichloromethane at room temperature to give novel silver(i)-nhc complexes. structures of these benzimidazolium salts and silver(i)-nhc complexes were characterized on the basis of elemental analysis, (1)h nmr, (13)c nmr, ir and lc-ms spectroscopic techniques. a series of benzimidazolium salts and silver(i)-nhc complexes were tested against standard bacterial ...201627301680
effect of electron-beam irradiation on antimicrobial, antibiofilm activity, and cytotoxicity of mouth rinses.oral health diseases are common in all regions of the world. mouth rinses are widely used generally by population as a port of daily oral care regimen. in addition to antimicrobial activity, mouth rinses possess certain cytotoxic effects. electron-beam (e-beam) radiation is a form of ionizing energy known to induce structural, physical, and chemical changes in irradiated products. in this study, the modulatory effects of e-beam in irradiated mouth rinses were evaluated for its biological activit ...201627237203
action of chlorhexidine, zingiber officinale, and calcium hydroxide on candida albicans, enterococcus faecalis, escherichia coli, and endotoxin in the root canals.the purpose of this in vitro study was to evaluate the antimicrobial activity of 2% chlorhexidine gel (chx) as auxiliary chemical substance and intracanal medications on candida albicans, enterococcus faecalis, escherichia coli, and their endotoxins in the root canals.201627206998
pregnane receptor gene polymorphisms, pathogenic bacteria distribution and drug sensitivity, and tcm syndrome differentiation in patients with cholelithiasis.to investigate the distribution of pathogens and drug resistance in bile and the association between the pregnane x receptor (pxr) gene polymorphisms, traditional chinese medicine (tcm) syndromes and the risk of cholesterol gallstone disease (cgd).201627086146
[clinical analysis for patients with continuous ambulatory peritoneal dialysis associated peritonitis].to analyze the clinical characteristics of continuous ambulatory peritoneal dialysis (capd) associated peritonitis in the tertiary hospitals and to discuss the preventive and therapeutic strategy.
 methods: the clinical characteristics, pathogens, resistance and outcomes of 126 capd associated peritonitis in 104 patients from jan, 2013 to june, 2016, were retrospectively analyzed.
 results: among the patients, the incidence rates of abdominal pain, fever, diarrhea and emesis were 104 (82.54%), 5 ...201628070047
antimicrobial and cytotoxicity evaluation of colloidal chitosan - silver nanoparticles - fluoride nanocomposites.the present study aimed to evaluate the antimicrobial activity and cytotoxicity of colloidal chitosan - silver nanoparticle - fluoride nanocomposites (cchagnpfnc), with different silver nanoparticle shapes and sizes. the syntheses of cchagnpfnc were performed with silver nitrate added to a chitosan solution, addition of a sodium borohydride solution and solid sodium fluoride. solution of ascorbic acid was added to synthesize larger silver nanoparticles. cchagnpfnc obtained: s1- 100% spherical, 8 ...201627642129
fabrication of antimicrobial silver-doped carbon structures by combinatorial pulsed laser deposition.we report on the selection by combinatorial pulsed laser deposition of silver-doped carbon structures with reliable physical-chemical characteristics and high efficiency against microbial biofilms. the investigation of the films was performed by scanning electron microscopy, high resolution atomic force microscopy, energy dispersive x-ray spectroscopy, x-ray diffraction, raman spectroscopy, bonding strength "pull-out" tests, and surface energy measurements. in vitro biological assays were carrie ...201627773854
extracellular vesicle-derived protein from bifidobacterium longum alleviates food allergy through mast cell suppression.the incidence of food allergies has increased dramatically during the last decade. recently, probiotics have been studied for the prevention and treatment of allergic disease.201626433560
photoinactivation of eight health-relevant bacterial species: determining the importance of the exogenous indirect mechanism.it is presently unknown to what extent the endogenous direct, endogenous indirect, and exogenous indirect mechanisms contribute to bacterial photoinactivation in natural surface waters. in this study, we investigated the importance of the exogenous indirect mechanism by conducting photoinactivation experiments with eight health-relevant bacterial species (bacteroides thetaiotaomicron, campylobacter jejuni, enterococcus faecalis, escherichia coli k12, e. coli o157:h7, salmonella enterica serovar ...201627121126
bacterially-associated transcriptional remodelling in a distinct genomic subtype of colorectal cancer provides a plausible molecular basis for disease development.the relevance of specific microbial colonisation to colorectal cancer (crc) disease pathogenesis is increasingly recognised, but our understanding of possible underlying molecular mechanisms that may link colonisation to disease in vivo remains limited. here, we investigate the relationships between the most commonly studied crc-associated bacteria (enterotoxigenic bacteroides fragilis, pks+ escherichia coli, fusobacterium spp., afac+ e. coli, enterococcus faecalis & enteropathogenic e. coli) an ...201627846243
association of oncogenic bacteria with colorectal cancer in south china.to quantify fusobacterium spp., enterococcus faecalis (e.faecalis), enterotoxigenic bacteroides fragilis (etbf), and enteropathogenic escherichia coli in colorectal cancer (crc) patients and their possible association with crc clinicopathogical features, we collected the resected tumors and adjacent normal tissues (n) from 97 crc patients. 48 age- and sex-matched healthy controls (hc) were also recruited. real-time pcr was used for bacterial quantification. the median abundance offusobacterium s ...201627821805
bacteriological examination of the abdominal effusion in bacterial peritonitis.this study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (api20e, api staph, api strep, api ana, biomerieux). rapid tests for detection of oxidase and catalase activity were also used. suscepti ...201627770528
increased enterococcus faecalis infection is associated with clinically active crohn disease.this study was performed to investigate the relationship between the abundance of pathogenic gut microbes in chinese patients with inflammatory bowel disease (ibd) and disease severity.we collected clinical data and fecal samples from 47 therapy-naive chinese patients with ulcerative colitis (uc), 67 patients with crohn disease (cd), and 48 healthy volunteers. bacteria levels of fusobacterium species (spp), enterotoxigenic bacteroides fragilis (b fragilis), enteropathogenic escherichia coli (e c ...201627684872
can surgical need in patients with naja atra (taiwan or chinese cobra) envenomation be predicted in the emergency department?to investigate the clinical predictors and the aetiologies for surgery in patients with naja atra (taiwan or chinese cobra) envenomation.201627516567
gut microbiota imbalance and colorectal cancer.the gut microbiota acts as a real organ. the symbiotic interactions between resident micro-organisms and the digestive tract highly contribute to maintain the gut homeostasis. however, alterations to the microbiome caused by environmental changes (e.g., infection, diet and/or lifestyle) can disturb this symbiotic relationship and promote disease, such as inflammatory bowel diseases and cancer. colorectal cancer is a complex association of tumoral cells, non-neoplastic cells and a large amount of ...201626811603
antibacterial effects of curcumin: an in vitro minimum inhibitory concentration study.to evaluate the antibacterial effect of curcumin with the minimum inhibitory concentration (mic) method in standard bacterial strains.201624097361
laserpitium zernyi hayek flower and herb extracts: phenolic compounds, and anti-edematous, antioxidant and antimicrobial activities.phenolic compounds and different biological activities of the dry methanol extracts of the flowers and the herb (aerial parts without flowers) of laserpitium zernyi hayek (apiaceae) were investigated. the total phenolic contents in the extracts were determined spectrophotometrically using folin-ciocalteu reagent. in both extracts, apigenin, luteolin, their 7-o-glucosides and chlorogenic acid were detected by hplc. identified phenolics were quantified in both extracts, except luteolin in l. zerny ...201627981797
survival of antibacterial resistance microbes in hospital-generated recycled wastewater.hospital wastewater has the potential to be a threat to the hospital environment as it can contain pathogenic bacteria that may facilitate the resistant nature of organisms within effluent or water treatment plants. the recycling of hospital wastewater should have good quality. this study was carried out to highlight the incidence of antibiotic resistant bacteria in hospital-generated recycled water. this study was conducted in a tertiary care teaching hospital during june 2013-june 2014. one hu ...201627959872
antimicrobial efficacy of complete denture cleansers.to evaluate the in vitro antimicrobial efficacy of alkaline peroxides against microbial biofilms on acrylic resin surfaces.201627505991
inoculation of tannin-degrading bacteria into novel hosts increases performance on tannin-rich diets.it has been hypothesized that herbivores host tannin-degrading bacteria (tdb) to overcome the toxic challenges posed by plant tannins. while tdb have been isolated from the guts of numerous mammals, their functional significance to their hosts has never been explicitly tested. we introduced tdb into lab rats, which do not host tdb, and measured host performance on tannin-rich diets. we first isolated three species of tdb, escherichia coli, bacillus subtilis and enterococcus faecalis, from the gu ...201625753857
enhancing antimicrobial activity of tio2/ti by torularhodin bioinspired surface modification.implant-associated infections are a major cause of morbidity and mortality. this study was performed using titanium samples coated by anodization with a titanium dioxide (tio2) shielded nanotube layer. tio2/ti surface was modified by simple immersion in torularhodin solution and by using a mussel-inspired method based on polydopamine as bio adhesive for torularhodin immobilization. sem analysis revealed tubular microstructures of torularhodin and the pda ability to function as a catchy anchor be ...201626414412
studies on bioactivity and secondary metabolites of crude extracts of bidens pilosa l. (asteraceae): a medicinal plant used in the transkei region of south africa.whole plant-parts of bidens pilosa were powdered and extracted in concentrated hexane, acetone, ethanol, methanol and water. the extracts were tested for antimicrobial activity against escherichia coli (25922), bacillus subtilis (atcc 6051), enterococcus faecalis (51299) staphylococcus aureus (atcc 29213) and pseudomonas aeruginosa (atcc127853), using standard microbiological techniques. active crude extracts were macerated in concentrated methanol and tested for secondary metabolites including ...201627166532
effect of probiotics on the incidence of ventilator-associated pneumonia in critically ill patients: a randomized controlled multicenter trial.to evaluate the potential preventive effect of probiotics on ventilator-associated pneumonia (vap).201627043237
ppgpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in gram-positive bacteria.the stringent response is a survival mechanism used by bacteria to deal with stress. it is coordinated by the nucleotides guanosine tetraphosphate and pentaphosphate [(p)ppgpp], which interact with target proteins to promote bacterial survival. although this response has been well characterized in proteobacteria, very little is known about the effectors of this signaling system in gram-positive species. here, we report on the identification of seven target proteins for the stringent response nuc ...201626951678
chemical composition and antimicrobial activity of the essential oil and methanol extract of hypericum aegypticum subsp. webbii (spach) n. robson.hypericum aegypticum subsp. webbii is an evergreen shrub spread in mediterranean part of central and southeastern europe. the chemical composition and antimicrobial activity of the essential oil and meoh extract of h. aegypticum subsp. webbii were investigated. the monoterpenes α-pinene (63.4 - 68.5%) and β-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. in the cluster analysis, the essential oil of h. aegypticum subsp. webbii was separated and chemically differe ...201626923468
addressing the requirements of high-sensitivity single-molecule imaging of low-copy-number proteins in bacteria.single-molecule fluorescence super-resolution imaging and tracking provide nanometer-scale information about subcellular protein positions and dynamics. these single-molecule imaging experiments can be very powerful, but they are best suited to high-copy number proteins where many measurements can be made sequentially in each cell. we describe artifacts associated with the challenge of imaging a protein expressed in only a few copies per cell. we image live bacillus subtilis in a fluorescence mi ...201626888309
purification and characteristics of a novel bacteriocin produced by enterococcus faecalis l11 isolated from chinese traditional fermented cucumber.to purify and characterize a novel bacteriocin with broad inhibitory spectrum produced by an isolate of enterococcus faecalis from chinese fermented cucumber.201626875092
gut-colonizing bacteria promote c. elegans innate immunity by producing nitric oxide.many commensal bacteria in the gut are beneficial to the host immune system, but the underlying mechanisms are largely unclear. using culture-independent illumina miseq sequencing of the bacterial 16s rrna gene amplicons, we show that bacterial diversity in the intestine of caenorhabditis elegans, the free-living nematode, is distinct from that in soil. of these bacteria, bacillus subtilis is the most prominent species in the worm gut. we demonstrate that b. subtilis confers worm resistance to i ...201626854225
survival of bacteria of laboratory animal origin on cage bedding and inactivation by hydrogen peroxide vapour.this study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (hpv) on paper bedding pieces. bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (bsa) in water, and held in a mouse facility. viable counts showed variable survival rates over time for the bacterial species used ([pasteurella] pneumotropica, muribacter muris, pseudomonas aeruginosa, acinetobacter redioresistens ...201627932683
dental unit water treatment with hydrogen peroxide and monovalent silver ions artificially contaminated with freshly isolated pathogens.dental unit water (duw) could be contaminated by human pathogens coming from biological fluids penetrated during patient treatment and by opportunistic pathogens detached from aquatic biofilm. these microorganisms could be spread to following patients. we tested the disinfectant activity of hydrogen peroxide and monovalent silver ions (h(2)o(2)-ag(+)) into duw artificially contaminated with freshly isolated pathogens.201626835793
use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats.human are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. although the digestive system is the first organ to come into contact with food contaminants, very few data are available on the impact of low-dose pesticide exposure during the in utero and postnatal periods on intestinal bacterial translocation (bt). previous studies have revealed that chlorpyrifos (cpf) exposure is associated with intestinal dysbiosis and the conta ...201627826358
chemical variability and biological activities of essential oils of micromeria inodora (desf.) benth. from algeria.the chemical composition of the essential oils isolated from the aerial parts of micromeria inodora (desf.) benth. collected in 24 algerian localities was investigated from the first time using gc-fid, gc/ms and (13) c-nmr. altogether, 83 components which accounted for 94.7% of the total oil composition were identified. the main compounds were trans-sesquisabinene hydrate (1; 20.9%), α-terpinyl acetate (2; 19.8%), globulol (3; 4.9%), caryophyllene oxide (4; 4.3%), β-bisabolol (5; 2.9%) and trans ...201627448034
biosynthesis of palladium nanoparticles by using moringa oleifera flower extract and their catalytic and biological properties.the biosynthesis of nanostructured biopalladium nanoparticles (pdnps) from an aqueous solution of crystalline palladium acetate is reported. for the synthesised pdnps in solution, an agroforest biomass waste petal of moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. continuous absorption in the uv region and subsequent brown colour change confirmed the formation of pdnps. a strong surface plasmon peak for pdnps occurred at 460nm. pdnps were characterized ...201627776261
myrcia ovata cambessedes essential oils: a proposal for a novel natural antimicrobial against foodborne bacteria.this paper reports the innovative antibacterial activity of essential oils (eos) from nine myrcia ovata cambessedes plants against eight foodborne bacteria. staphylococcus aureus, bacillus cereus, bacillus subtilis, enterococcus faecalis and pseudomonas aeruginosa were the most susceptible bacteria to eos. in particular, the p. aeruginosa, which is usually resistant to antimicrobials agents, was extremely sensitive to some eos. the gram-positive and gram-negative bacteria were inhibited and elim ...201627554278
evaluating the efficiency of humic acid to remove micro-organisms from denture base material.to evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials.201625523597
enhanced efficacy of clindamycin hydrochloride encapsulated in pla/plga based nanoparticle system for oral delivery.clindamycin hydrochloride (clh) is a clinically important oral antibiotic with wide spectrum of antimicrobial activity that includes gram-positive aerobes (staphylococci, streptococci etc.), most anaerobic bacteria, chlamydia and certain protozoa. the current study was focused to develop a stabilised clindamycin encapsulated poly lactic acid (pla)/poly (d,l-lactide-co-glycolide) (plga) nano-formulation with better drug bioavailability at molecular level. various nanoparticle (nps) formulations o ...201627463797
chemical profile, antioxidant and antibacterial activities of achillea moschata wulfen, an endemic species from the alps.aerial parts of achillea moschata wulfen (asteraceae) growing wild in the italian rhaetian alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. inspection of the metabolic profile combining hplc-dad and esi-ms/ms data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. among them, the major compound was 7-o-glucosyl apigenin. caffeoyl derivates were other phen ...201627347915
formation and biopharmaceutical characterization of electrospun pvp mats with propolis and silver nanoparticles for fast releasing wound dressing.antibacterial, antiviral, antifungal, antioxidant, anti-inflammatory, and anticancer activities of propolis and its ability to stimulate the immune system and promote wound healing make it a proper component for wound dressing materials. silver nanoparticles are recognized to demonstrate strong antiseptic and antimicrobial activity; thus, it also could be considered in the development of products for wound healing. combining propolis and silver nanoparticles can result in improved characteristic ...201626981531
isolation and structural elucidation of brevibacillin, an antimicrobial lipopeptide from brevibacillus laterosporus that combats drug-resistant gram-positive bacteria.a new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant staphylococcus aureus, vancomycin-resistant strains of enterococcus faecalis and lactobacillus plantarum, and other gram-positive bacteria. the producer strain, designated osy-i1, was determined to be brevibacillus laterosporusvia morphological, biochemical, and genetic analyses. the antimicrobial agent was extracted from cells of osy-i1 with isopropanol, purified by high-performance ...201626921428
antibacterial effects of alchornea cordifolia (schumach. and thonn.) müll. arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens.the leaves, stems and roots of alchornea cordifolia (schumach. and thonn.) müll. arg. are used as traditional medicine in many african countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds.201626724423
antimicrobial activity of kefir against various food pathogens and spoilage bacteria.kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ...201628115890
in vitro evaluation of the antimicrobial ability and cytotoxicity on two melanoma cell lines of a benzylamide derivative of maslinic acid.maslinic acid is a pentacyclic triterpene extracted from olives that has been systematically reported to exert several therapeutic effects, such as antitumoral, antidiabetic, antioxidant, anti-inflammatory, antiparasitic, and antiviral properties. recently, new derivatives of maslinic acid have been obtained and expanded the spectrum of biological activities and improved the existing ones. the present study was meant to perform the in vitro assessment of the (i) cytotoxic effects of a benzylamid ...201628050335
molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia.endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems.201627099679
development of nitrocellulose membrane filters impregnated with different biosynthesized silver nanoparticles applied to water purification.bactericidal water filters were developed. for this purpose, nitrocellulose membrane filters were impregnated with different biosynthesized silver nanoparticles. silver nanoparticles (agnps) from aspergillus niger (agnps-asp), cryptococcus laurentii (agnps-cry) and rhodotorula glutinis (agnps-rho) were used for impregnating nitrocellulose filters. the bactericidal properties of these nanoparticles against escherichia coli, enterococcus faecalis and pseudomona aeruginosa were successfully demonst ...201626695258
chemical profiling, antimicrobial and insecticidal evaluations of polygonum hydropiper l.the emergence of multidrug resistant (mdr) pathogens is of great concern to the global health community. our ability to effectively treat diseases is based on the discovery of potent drugs for the treatment of these challenging diseases. traditional medicines are one of the major sources for the discovery of safe, effective and economical drug candidates. in order to validate its antibacterial, antifungal and insecticidal potentials with respect to traditional uses, we have screened for the firs ...201627919287
antibacterial and antifungal potentials of the solvents extracts from eryngium caeruleum, notholirion thomsonianum and allium consanguineum.herbal medicines have long been used for various ailments in various societies and natural bioactive compounds are gaining more and more importance due to various factors. in this context, three plant species i.e., eryngium caeruleum, notholirion thomsonianum and allium consanguineum have been aimed for the scientific verification of their purported traditional uses against various infectious diseases.201627881119
effect of polymer/nanosilver composite packaging on long-term microbiological status of iranian saffron (crocus sativus l.).crocus sativus l. (saffron) is a valuable plant which is native to iran. saffron is the dried stigmata of the flowering part of the plant that is usually contaminated with different bacteria and fungi through production process. antimicrobial properties of silver nanoparticles are well recognized. to survey the effects of nanosilver packaging on microbiological status of spiked, saffron samples over a six month period were chosen. saffron samples from five regions of khorasan province were purch ...201627081358
antimicrobial activity of new 2-thioxo-benzo[g]quinazolin-4(3h)-one derivatives.the antimicrobial activity of a synthesized series of 28 2-thioxo-benzo[g]quinazolin-4(3h)-one derivatives was evaluated in vitro against five gram-positive bacteria, including bacillus subtilis, enterococcus faecalis, staphylococcus aureus, staphylococcus epidermidis and streptococcus pyogenes. the antibacterial activity was extended to include five gram-negative bacteria: pseudomonas aeruginosa, escherichia coli, proteus mirabilis, klebsiella oxytoca and enterobacter cloacae. furthermore, the ...201627306606
antimicrobial activity of synthesized 2-methylthiobenzo[g][1,2,4]- triazolo[1,5-a]quinazoline derivatives.the present study was carried out to evaluate the antimicrobial activity of a synthesized 2-methylthio-benzo[g][1,2,4]triazolo[1,5- a]quinazoline series. the compounds (1-21) were tested against a variety of gram-positive bacterial species including bacillus subtilis (rcmb 01001 69-3), enterococcus faecalis (rcmb 0100154-2), staphylococcus aureus (rcmb 0100183-9), staphylococcus epidermidis (rcmb 0100183-9) and streptococcus pyogenes (rcmb 0100172-5). in addition, gram-negative bacteria were als ...201627140181
synthesis of newer 1,2,3-triazole linked chalcone and flavone hybrid compounds and evaluation of their antimicrobial and cytotoxic activities.the present study was carried out in an attempt to synthesize a new class of antimicrobial and antiplasmodial agents by copper catalyzed click chemistry to afford 25 compounds 10-14(a-e) of 1,4-disubstituted-1,2,3-triazole derivatives of chalcones and flavones. the structures of the newly synthesized compounds were established by elemental analysis, ir, (1)h nmr, (13)c nmr and mass spectral data. the newly synthesized compounds were evaluated for their antibacterial activity against gram positiv ...201626922227
chemical constituents from campylotropis hirtella.a phytochemical investigation on the roots of campylotropis hirtella afforded nine new isoflavones (3-9, 12, 15), two new isoflavans (10 and 11), one new coumestan (1), and three new prenylated benzoic acid derivatives (2, 13, 14), together with twenty-four known compounds. their structures were established by spectroscopic analysis and circular dichroism data. the isolated compounds were also evaluated for their antibacterial activities against enterococcus faecalis, salmonella gallinarum, stre ...201627220079
epigenetic activation of antibacterial property of an endophytic streptomyces coelicolor strain azra 37 and identification of the induced protein using maldi tof ms/ms.the endophytic streptomyces coelicolor strain azra 37 was isolated from the surface sterilized root of azadirachta indica a. juss., commonly known as neem plant in india. since only a few reports are available regarding epigenetic modulations of microbial entities, s. coelicolor was treated with different concentrations of 5-azacytidine for this purpose and evaluated for its antibacterial potential against five human pathogenic bacteria (aeromonas hydrophila ims/gn11, enterococcus faecalis ims/g ...201626844762
effect of bioactive dental adhesive on periodontal and endodontic pathogens.the objectives of this study were to: (1) develop a new bioactive dental bonding agent with nanoparticles of amorphous calcium phosphate and dimethylaminohexadecyl methacrylate for tooth root caries restorations and endodontic applications, and (2) investigate biofilm inhibition by the bioactive bonding agent against eight species of periodontal and endodontic pathogens for the first time. bonding agent was formulated with 5 % of dimethylaminohexadecyl methacrylate. nanoparticles of amorphous ca ...201627699574
novel bioactive nanocomposite for class-v restorations to inhibit periodontitis-related pathogens.the occurrence of tooth root caries is increasing as the world population ages and tooth retention in seniors increases. class v restorations with subgingival margins are difficult to clean and often lead to periodontitis. the objectives of this study were to develop a class v composite containing dimethylaminohexadecyl methacrylate (dmahdm) and nanoparticles of amorphous calcium phosphate (nacp), and investigate mechanical properties and the inhibition of six species of periodontitis-related bi ...201627671471
development of novel electrospun dual-drug fiber mats loaded with a combination of ampicillin and metronidazole.our study was performed with the aim of preparing electrospun polylactide fibers with a combination of ampicillin (amp) and metronidazole (mnz) and investigating their drug release behavior and the antibacterial effect on aggregatibacter actinomycetemcomitans and other oral pathogens.201627262200
antibacterial effect of silver (i) carbohydrate complexes on oral pathogenic key species in vitro.it was the aim of this study to evaluate the antibacterial impact of two silver(i) carbohydrate complexes with tripodal thioglycosides, namely tris[2-(β-d-thio-glucopyranosyl)ethyl]-amine-silver(i)-nitrate (3) and tris[2-(α-d-thio-manno-pyranosyl)ethyl]-amine-silver(i)-nitrate (4), on five oral pathogenic bacterial strains. furthermore, cytocompatibility was tested using human gingival fibroblasts (hgf).201627009305
the starvation resistance and biofilm formation of enterococcus faecalis in coexistence with candida albicans, streptococcus gordonii, actinomyces viscosus, or lactobacillus acidophilus.enterococcus faecalis is the most frequently detected species in root canal-treated teeth, and it is able to survive under starvation conditions. however, persistent periapical disease is often caused by multispecies. the aim of this study was to explore the survival of e. faecalis in starvation conditions and biofilm formation with the 4 common pathogenic species.201627316318
lactic acid microbiota identification in water, raw milk, endogenous starter culture, and fresh minas artisanal cheese from the campo das vertentes region of brazil during the dry and rainy seasons.minas artisanal cheese, produced in the campo das vertentes region of brazil, is made from raw milk and endogenous starter cultures. although this cheese is of great historical and socioeconomic importance, little information is available about its microbiological and physical-chemical qualities, or about its beneficial microbiota. this work was aimed at evaluating the qualities of the cheese and the components used for its production, comparing samples collected during the dry and rainy seasons ...201627289151
the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study.this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ...201626949701
development of a cavity disinfectant containing antibacterial monomer mdpb.an experimental cavity disinfectant (acc) that is intended to be used for various direct and indirect restorations was prepared by adding an antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (mdpb) at 5% into 80% ethanol. the antibacterial effectiveness of acc and its influences on the bonding abilities of resin cements were investigated. to examine the antibacterial activity of unpolymerized mdpb, the minimum inhibitory and bactericidal concentrations (mic and mbc) were determine ...201627558263
antibacterial effects of n-acetylcysteine against endodontic pathogens.the success of endodontic treatment depends on the eradication of microorganisms from the root canal system and the prevention of reinfection. the purpose of this investigation was to evaluate the antibacterial and antibiofilm efficacy of n-acetylcysteine (nac), an antioxidant mucolytic agent, as an intracanal medicament against selected endodontic pathogens. minimum inhibitory concentrations (mics) of nac for actinomyces naeslundii, lactobacillus salivarius, streptococcus mutans, and enterococc ...201627033208
a three-year survey of the antimicrobial resistance of microorganisms at a chinese hospital.the present retrospective study aimed to investigate the antibiotic susceptibility of bacteria at shanghai first people's hospital (shanghai, china) between 2009 and 2011. an increasing trend of antibiotic resistance was observed in this hospital between 2009 and 2011. escherichia coli, acinetobacter baumannii and staphylococcus aureus were the most prevalent resistant strains. antimicrobial susceptibility was detected using standard kirby-bauer disk diffusion and analyzed using world health org ...201626997986
pilot screening to determine antimicrobial synergies in a multidrug-resistant bacterial strain library.with the rise in multidrug-resistant (mdr) bacterial infections, there has been increasing interest in combinations of ≥2 antimicrobial agents with synergistic effects. we established an mdr bacterial strain library to screen for in vitro antimicrobial synergy by using a broth microdilution checkerboard method and high-throughput luciferase-based bacterial cell viability assay. in total, 39 mdr bacterial strains, including 23 carbapenem-resistant gram-negative bacteria, 9 vancomycin-intermediate ...201626974861
laryngoscopes: evaluation of microbial load of blades.laryngoscope blades were analyzed, and the presence of blood, bodily fluids, and microorganisms was verified, indicating their potential as a source of cross contamination during clinical usage. the way in which the blades are cleaned and disinfected in daily practice may place the patient and health care team at risk. the aim of this study was to determine the bacterial and fungal load on this equipment.201626775934
antibacterial properties of tebipenem pivoxil tablet, a new oral carbapenem preparation against a variety of pathogenic bacteria in vitro and in vivo.to systemically investigate the in vitro and in vivo antibacterial properties of tebipenem pivoxil tablet. in addition, acute toxicity of this preparation was also studied.201626751436
antibiotic susceptibility of commensal bacteria from human milk.recent studies have focused on foodborne or commensal bacteria as vehicles of antibiotic resistance. however, the antibiotic resistance of milk bacteria from healthy donors is still vague in taiwan. for this purpose, human milk samples were obtained from randomly recruited 19 healthy women between 3 and 360 days post-partum. antibiotic susceptibility profile of bacteria from milk samples was determined. about 20 bacterial species were isolated from milk samples including staphylococcus (6 specie ...201626494365
silver nanocomposite biosynthesis: antibacterial activity against multidrug-resistant strains of pseudomonas aeruginosa and acinetobacter baumannii.bacterial resistance is an emerging public health issue that is disseminated worldwide. silver nanocomposite can be an alternative strategy to avoid gram-positive and gram-negative bacteria growth, including multidrug-resistant strains. in the present study a silver nanocomposite was synthesized, using a new green chemistry process, by the addition of silver nitrate (1.10(-3) mol·l(-1)) into a fermentative medium of xanthomonas spp. to produce a xanthan gum polymer. transmission electron microsc ...201627657031
antimicrobial susceptibility among important pathogens collected as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) in spain, 2004-2014.here we report in vitro activity data from the tigecycline evaluation and surveillance trial (t.e.s.t.) for tigecycline and comparators against gram-positive and gram-negative organisms collected from 27 medical centres in spain between 2004 and 2014. minimum inhibitory concentrations (mics) were determined according to the broth microdilution methodology of the clinical and laboratory standards institute (clsi) and susceptibility were determined according to european committee on antimicrobial ...201627530839
[clinical analysis of 67 cases of surgical site infection after spine surgery].to investigate the treatment of surgical site infection(ssi) after spine surgery.201627373479
antimicrobial activity against a global collection of skin and skin structure pathogens: results from the tigecycline evaluation and surveillance trial (t.e.s.t.), 2010-2014.as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) we report antimicrobial resistance among gram-positive and gram-negative isolates collected globally from integumentary sources between 2010 and 2014.201627343986
silver nanoparticles: biosynthesis using an atcc reference strain of pseudomonas aeruginosa and activity as broad spectrum clinical antibacterial agents.currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobial properties of these ones. this study reports the extracellular biosynthesis of silver nanoparticles (ag-nps) using a pseudomonas aeruginosa strain from a reference culture collection. a greenish culture supernatant of p. aeruginosa incubated at 37°c with a silver nitrate solution for 24 h changed to a yellowish brown color, indicating the formation of ag-nps, which was conf ...201627340405
pathogen distribution and drug resistance of nephrology patients with urinary tract infections.pathogen distribution characteristics of nephrology patients with urinary tract infections are studied, and drug resistance of nephrology and urinary tract infection disease are analyzed, so as to provide sufficient evidence for treatment of patients.201627275125
diversity of culturable thermophilic actinobacteria in hot springs in tengchong, china and studies of their biosynthetic gene profiles.the class actinobacteria has been a goldmine for the discovery of antibiotics and has attracted interest from both academics and industries. however, an absence of novel approaches during the last few decades has limited the discovery of new microbial natural products useful for industries. scientists are now focusing on the ecological aspects of diverse environments including unexplored or underexplored habitats and extreme environments in the search for new metabolites. this paper reports on t ...201627048448
bioactivities of ethanolic extract and its fractions of cistus laurifolius l. (cistaceae) and salvia wiedemannii boiss. (lamiaceae) species.cistus laurifolius l. (cistaceae) and salvia wiedemannii boiss. (lamiaceae) have been used for treatment of some illnesses in turkish folk medicine. in the present study, the ethanolic extract and its fractions obtained using re-extraction by hexane (hx), chloroform (chcl3), butanol, and remaining-water (r-h2o) of c. laurifolius were screened for their in vitro bioactivities.201627041865
bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens.using direct current magnetron sputtering (dcms), we generated flexible copper polyester surfaces (cu-pes) and investigated their antimicrobial activity against a range of multidrug-resistant (mdr) pathogens including eight gram-positive isolates (three methicillin-resistant staphylococcus aureus [mrsa], four vancomycin-resistant enterococci, one methicillin-resistant staphylococcus epidermidis) and four gram-negative strains (one extended-spectrum β-lactamase-producing [esbl] escherichia coli, ...201627020284
bacteraemia during transurethral resection of the prostate: what are the risk factors and is it more common than we think?the aim of this work was to investigate the microbial causes, incidence, duration, risk factors and clinical implications of bacteraemia occurring during transurethral resection of the prostate (turp) surgery to better inform prophylaxis strategies. an ethically approved, prospective, cohort study of patients undergoing turp was conducted. clinical information and follow-up details were collected using standardized data collection sheets. blood was obtained for culture at 6 different time points ...201627391962
fosfomycin: a first-line oral therapy for acute uncomplicated cystitis.fosfomycin is a new agent to canada approved for the treatment of acute uncomplicated cystitis (auc) in adult women infected with susceptible isolates of e. coli and enterococcus faecalis. we reviewed the literature regarding the use of oral fosfomycin for the treatment of auc. all english-language references from 1975 to october 2015 were reviewed. in canada, fosfomycin tromethamine is manufactured as monurol® and is available as a 3-gram single dose sachet. fosfomycin has a unique chemical str ...201627366158
lipid composition in a strain of bacillus subtilis, a producer of iturin a lipopeptides that are active against uropathogenic bacteria.urinary tract infections are a common disease in humans. therefore, new methods are needed to destroy biofilms that are formed by uropathogens. iturin a lipopeptides (lps) c14 and c15 are potent biosurfactants synthetized by the bacillus subtilis i'1a strain. the biological activity of extracted lps was confirmed by examining extracts from i'1a cultures against uropathogenic bacteria that had been isolated from biofilms on urinary catheters. compared with cultures of dsm 3257, which produce surf ...201627550437
newer antibiotics for the treatment of peritoneal dialysis-related peritonitis.peritonitis is a debilitating infectious complication of peritoneal dialysis (pd). drug-resistant bacterial peritonitis typically has a lower response rate to antibiotics. in the past 15 years, newer antibiotics with activities against drug-resistant gram-positive bacteria have been developed. in most circumstances, peritonitis due to methicillin-resistant staphylococci responds to vancomycin. if vancomycin cannot be used due to allergy and/or non-susceptibility, there is increasing evidence tha ...201627478608
discovery and analysis of natural-product compounds inhibiting protein synthesis in pseudomonas aeruginosa.bacterial protein synthesis is the target for numerous natural and synthetic antibacterial agents. we have developed a poly(u) mrna-directed aminoacylation/translation (a/t) protein synthesis system composed of phenylalanyl-trna synthetases (phers), ribosomes, and ribosomal factors from pseudomonas aeruginosa this system has been used for high-throughput screening of a natural-compound library. assays were developed for each component of the system to ascertain the specific target of inhibitory ...201627246774
composition and dynamics of the respiratory tract microbiome in intubated patients.lower respiratory tract infection (lrti) is a major contributor to respiratory failure requiring intubation and mechanical ventilation. lrti also occurs during mechanical ventilation, increasing the morbidity and mortality of intubated patients. we sought to understand the dynamics of respiratory tract microbiota following intubation and the relationship between microbial community structure and infection.201626865050
development of a novel test for simultaneous bacterial identification and antibiotic susceptibility.background. elucidation of a pathogen's antimicrobial susceptibility requires subculture after the organism is first isolated. this takes several days, requiring patients to be treated with broad-spectrum antibiotics. this approach contributes to the development of bacterial resistance. methods. microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. bacterial suspensions were added in the presence/absence of selected antibiotics. after washing, captured bacte ...201627872553
functional characterization of triclosan-resistant enoyl-acyl-carrier protein reductase (fabv) in pseudomonas aeruginosa.pseudomonas aeruginosa is extremely resistant to triclosan. previous studies have shown that p. aeruginosa encodes a triclosan-resistant enoyl-acyl-carrier protein reductase (enr), fabv, and that deletion of fabv causes p. aeruginosa to be extremely sensitive to triclosan. in this report, we complemented a p. aeruginosa fabv deletion strain with several triclosan-resistant enr encoding genes, including vibrio cholerae fabv, bacillus subtilis fabl and enterococcus faecalis fabk. all complemented ...201627965638
antibiotic treatment and resistance in chronic wounds of vascular origin.the problem of antibiotic resistance is worldwide and affects many types of pathogens. this phenomenon has been growing for decades and nowadays we are faced with a wide range of worrisome pathogens that are becoming resistant and many pathogens that may soon be untreatable. the aim of this study was to determine the resistance and antibiotic treatment in chronic wounds of vascular origin.201627547055
drug and vaccine development for the treatment and prevention of urinary tract infections.urinary tract infections (uti) are among the most common bacterial infections in humans, affecting millions of people every year. uti cause significant morbidity in women throughout their lifespan, in infant boys, in older men, in individuals with underlying urinary tract abnormalities, and in those that require long-term urethral catheterization, such as patients with spinal cord injuries or incapacitated individuals living in nursing homes. serious sequelae include frequent recurrences, pyelon ...201626999391
antimicrobial effects of silver nanoparticles stabilized in solution by sodium alginate.to investigate the effect of nanosilver particles in solution stabilized in a matrix of sodium alginate on the growth and development of pathogenic bacteria such as staphylococcus aureus, enterococcus faecalis, escherichia coli, proteus vulgaris, enterobacter cloacae, the antibiotic-resistant strain of pseudomonas aeruginosa, the yeast-like fungus candida albicans, and the luminescent bacteria photobacterium leiognathi sh1.201627747311
antimicrobial effect of ozone made by kp syringe of high-frequency ozone generator.the aim of this study was to evaluate in vitro the antibacterial effect of ozone on suspension of three different bacteria inoculated in prepared canals of extracted human teeth.201627789911
anti-mrsa activities of enterocins dd28 and dd93 and evidences on their role in the inhibition of biofilm formation.methicillin-resistant staphylococcus aureus (mrsa) has become a worrisome superbug. this work aimed at studying the effects of two class iib bacteriocins, enterocins dd28 and dd93 as anti-mrsa agents. thus, these bacteriocins were purified, from the cultures supernatants of enterococcus faecalis 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. the anti-staphylococcal activity was shown ...201627303396
virb8-like protein trah is crucial for dna transfer in enterococcus faecalis.untreatable bacterial infections caused by a perpetual increase of antibiotic resistant strains represent a serious threat to human healthcare in the 21(st) century. conjugative dna transfer is the most important mechanism for antibiotic resistance and virulence gene dissemination among bacteria and is mediated by a protein complex, known as type iv secretion system (t4ss). the core of the t4ss is a multiprotein complex that spans the bacterial envelope as a channel for macromolecular secretion. ...201627103580
a novel mva-mediated pathway for isoprene production in engineered e. coli.to deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of great significance in that it is an important platform chemical, which has been used in industrial production of synthetic rubber for tires and coatings or aviation fuel.201626786050
burkholderia paludis sp. nov., an antibiotic-siderophore producing novel burkholderia cepacia complex species, isolated from malaysian tropical peat swamp soil.a novel gram negative rod-shaped bacterium, designated strain msh1(t), was isolated from southeast pahang tropical peat swamp forest soil in malaysia and characterized using a polyphasic taxonomy approach. the predominant cellular fatty acids (>10.0%) were c16:0 (31.7%), c17:0 cyclo (26.6%), and c19:0 cyclo ω8c (16.1%). the polar lipids detected were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. the predominant ubiquinone was q-8. this revealed that strain msh1(t) b ...201628066367
characterization of antimicrobial substance from lactobacillus salivarius kl-d4 and its application as biopreservative for creamy filling.lactobacillus salivarius kl-d4 isolated from duck intestine produced bacteriocin which was stable at high temperature and a wide ph range of 3-10. its cell free supernatant at ph 5.5 exhibited wide inhibitory spectrum against both g+ and g- bacteria. the highest bacteriocin production was obtained in mrs broth supplemented with 0.5 % (w/v) caco3 at 6 h by gentle shaking. pcr walking using specific primers at the conserved region of class-ii bacteriocin resulted in 4 known genes of kld1, kld2, kl ...201627462508
host-specific functional significance of caenorhabditis gut commensals.the gut microbiota is an important contributor to host health and fitness. given its importance, microbiota composition should not be left to chance. however, what determines this composition is far from clear, with results supporting contributions of both environmental factors and host genetics. to gauge the relative contributions of host genetics and environment, specifically the microbial diversity, we characterized the gut microbiotas of caenorhabditis species spanning 200-300 million years ...201627799924
thusin, a novel two-component lantibiotic with potent antimicrobial activity against several gram-positive pathogens.due to the rapidly increasing prevalence of multidrug-resistant bacterial strains, the need for new antimicrobial drugs to treat infections has become urgent. bacteriocins, which are antimicrobial peptides of bacterial origin, are considered potential alternatives to conventional antibiotics and have attracted widespread attention in recent years. among these bacteriocins, lantibiotics, especially two-component lantibiotics, exhibit potent antimicrobial activity against some clinically relevant ...201627486447
functional analysis of the citrate activator cito from enterococcus faecalis implicates a divalent metal in ligand binding.the regulator of citrate metabolism, cito, from enterococcus faecalis belongs to the fcd family within the gntr superfamily. in the presence of citrate, cito binds to cis-acting sequences located upstream of the cit promoters inducing the expression of genes involved in citrate utilization. the quantification of the molecular binding affinities, performed by isothermal titration calorimetry (itc), indicated that cito has a high affinity for citrate (k d = 1.2 ± 0.2 μm), while it did not recogniz ...201626903980
the essential oils of rhaponticum carthamoides hairy roots and roots of soil-grown plants: chemical composition and antimicrobial, anti-inflammatory, and antioxidant activities.the essential oils were isolated by hydrodistillation from the hairy roots (hr) and roots of soil-grown plants (sgr) of rhaponticum carthamoides and were analyzed by gc-ms method. in the both essential oils 62 compounds were identified. the root essential oils showed the differences in the qualitative and quantitative composition. the sesquiterpene hydrocarbons (55-62%) dominated in both essential oils. the major compounds of hr essential oil were cyperene, 13-norcypera-1(5),11(12)-diene, and ca ...201628074117
functional analysis of a bacitracin resistance determinant located on icecp1, a novel tn916-like element from a conjugative plasmid in clostridium perfringens.bacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. they act by interfering with the biosynthesis of the bacterial cell wall. in this study, we analyzed an avian necrotic enteritis strain of clostridium perfringens that was resistant to bacitracin and produced netb toxin. we identified a bacitracin resistance locus that resembled a bacitracin resistance determinant from enterococcus faecalis. it contained the structural genes bcrabd and a putative reg ...201526282424
identification of enterococcus faecalis antigens specifically expressed in vivo.molecular mechanism of the pathogenicity of enterococcus faecalis (e. faecalis), a suspected endodontic pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. here we report the identification of in vivo expressed antigens of e. faecalis by using a novel immunoscreening technique called change-mediated antigen technology (cmat) and an experimental animal model of endodontic infection.201526587417
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