Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| antibiotic resistance patterns of bacterial strains isolated from periplaneta americana and musca domestica in tangier, morocco. | flies and cockroaches are two insects in close contact with human beings. they are carriers of human pathogenic bacteria on the external areas of their bodies or in their digestive tracts. this study examines periplaneta americana and musca domestica collected from the residential areas of six districts in tangier, morocco. | 2010 | 20440055 |
| species and strain identification of lactic acid bacteria using ftir spectroscopy and artificial neural networks. | lactic acid bacteria (lab) are a heterogeneous group of micro-organisms with high relevance for fermented food and beverages as well as human and animal health. identification of lab is complicated by species richness, a high degree of heterogeneity between species and the presence of very closely related species at the same time. in the present study we developed identification systems based on ftir spectra and artificial neural networks (anns) for species identification of 92 lab species out o ... | 2010 | 20422658 |
| global spread of the hyl(efm) colonization-virulence gene in megaplasmids of the enterococcus faecium cc17 polyclonal subcluster. | enterococcus faecium has increasingly been reported as a nosocomial pathogen since the early 1990s, presumptively associated with the expansion of a human-associated enterococcus faecium polyclonal subcluster known as clonal complex 17 (cc17) that has progressively acquired different antibiotic resistance (ampicillin and vancomycin) and virulence (esp(efm), hyl(efm), and fms) traits. we analyzed the presence and the location of a putative glycoside hydrolase hyl(efm) gene among e. faecium strain ... | 2010 | 20385861 |
| [detection of vancomycin-resistant enterococci using multiplex real-time pcr assay and melting curve analysis]. | we developed and evaluated the utility of a multiplex real-time pcr assay that uses melting curve analysis and allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. | 2010 | 20445331 |
| formulation of synthetic greywater as an evaluation tool for wastewater recycling technologies. | on-site greywater recycling is one of the main ways of preserving water resources in urban or arid areas. this study aims to formulate model synthetic greywater (sgw) in order to evaluate and compare the performances of several recycling processes on a reproducible effluent. the formulated sgw is composed of septic effluent to provide indicators of faecal contamination, and technical quality chemical products to simulate organic pollution of greywater. to ensure that the sgw developed is represe ... | 2010 | 20391806 |
| enterocins in food preservation. | the enterococcus genus, a member of the lactic acid bacteria (lab) is found in various environments, but more particularly in the intestines of humans and other animals. although sometimes associated with pathogenicity these bacteria have many benefits. they have been found in traditional artisanal fermented products, are used as probiotic cultures and nowadays extensively studied for the production of bacteriocins--the enterocins. many of these enterocins have been found to be active against li ... | 2010 | 20399522 |
| curvularia keratomycosis in a dog. | a 10-year-old, spayed female bichon frise was referred to the veterinary medical center of the university of florida with a 6-week history of blepharospasm and a nonhealing ulcer of the left eye. ophthalmic examination revealed a 3-4 mm diameter corneal ulcer with faint edema at the lesion edge, and a raised grayish area on the paraxial cornea near the 2 o'clock position. cytological testing revealed fungal hyphae and extracellular cocci. culture of the lesion found heavy growth of enterococcus ... | 2010 | 20447033 |
| novel anti-infective compounds from marine bacteria. | as a result of the continuous evolution of microbial pathogens towards antibiotic-resistance, there have been demands for the development of new and effective antimicrobial compounds. since the 1960s, the scientific literature has accumulated many publications about novel pharmaceutical compounds produced by a diverse range of marine bacteria. indeed, marine micro-organisms continue to be a productive and successful focus for natural products research, with many newly isolated compounds possessi ... | 2010 | 20411112 |
| expression of beta-defensin-3 in lungs of immunocompetent rats with methicillin-resistant staphylococcus aureus ventilator-associated pneumonia. | background: despite the rising incidence and high rate of treatment failure of ventilator-associated pneumonia (vap) due to methicillin-resistant staphylococcus aureus (mrsa), to date there has been no rat model specifically designed for antimicrobial evaluation. beta-defensin-3 acronym for beta-defensin-3 is correct as (bd-3) is an antimicrobial peptide and mainly expresses in the gastrointestinal and respiratory tract. it demonstrates a broad spectrum of potent antimicrobial activity against m ... | 2010 | 20400114 |
| [interpretive reading of the antibiogram in gram positive cocci]. | resistance to methicillin in staphylococcus is related to the expression of the meca gene, and involves resistance to all beta-lactams, with the exception of the new cephalosporins, ceftobiprole and ceftaroline. breakpoints for interpretation of this mechanism differ in s. aureus and in coagulase-negative species. for macrolides-lincosamides-streptogramins b, (mls(b)) the most frequent mechanism among resistant strains is expression of methylases (erm genes). topoisomerase changes caused by poin ... | 2010 | 20400208 |
| [investigation of the in vitro antibacterial effects of statins]. | it was previously shown that statins have anti-inflammatory, immunomodulatory and anti-oxidant effects. this study was aimed to investigate the in-vitro antibacterial effects of simvastatin and atorvastatin. in this study antibacterial activity of the statins were tested against 16 methicillin-susceptible stophylococcus aureus (mssa), 16 methicillin-resistant s.aureus (mrsa), 16 methicillin-resistant coagulase-negative staphylococcus (mrcons), 9 vancomycin-susceptible enterococcus faecium, 7 van ... | 2010 | 20455414 |
| in vitro studies of a novel antimicrobial luer-activated needleless connector for prevention of catheter-related bloodstream infection. | we report in vitro studies of a commercially available novel antimicrobial luer-activated connector with the inner surface coated with nanoparticle-silver to prevent contaminants from forming biofilm and causing catheter-related bloodstream infection. | 2010 | 20455695 |
| attachment of escherichia coli and enterococci to particles in runoff. | association of escherichia coli and enterococci with particulates present in runoff from erodible soils has important implications for modeling the fate and transport of bacteria from agricultural sources and in the selection of management practices to reduce bacterial movement to surface waters. three soils with different textures were collected from the ap horizon (silty loam, silty clay loam, and loamy fine sand), placed in portable box plots, treated with standard cowpats, and placed under a ... | 2010 | 20400597 |
| isolation and characterisation of bacteria from the haloalkaline lake elmenteita, kenya. | culture-independent studies show that soda lake environments harbour diverse groups of bacteria and archaea. in this study different enrichment and isolation media were used in an attempt to isolate novel groups of bacteria from lake elmenteita. different media were prepared using filter-sterilised water from the lake. the isolates recovered were purified on tryptic soy agar supplemented with 1% sodium carbonate and 4% sodium chloride. phylogenetic analysis of 181 partial 16s rrna gene sequences ... | 2010 | 20401502 |
| antibacterial effects of mdpb against anaerobes associated with endodontic infections. | to investigate the antibacterial effects of 12-methacryloyloxydodecylpyridinium bromide (mdpb), an antibacterial monomer synthesized by combining quaternary ammonium with a methacryloyl group, against three anaerobes associated with endodontic infections using planktonic and biofilm cells. | 2010 | 20456514 |
| clostridium difficile infection at a medical center in southern taiwan: incidence, clinical features and prognosis. | an increase in incidence of clostridium difficile infection (cdi) among western countries has been noted in recent years. epidemiological data of cdi are scarce in taiwan. this study is intended to depict the clinical features of cdi at a medical center in southern taiwan. | 2010 | 20457428 |
| effect of overnight storage of blood culture bottles on bacterial detection time in the bactec 9240 blood culture system. | identifying the pathogens present in blood stream infections is crucial to initiate appropriate antimicrobial therapy and avoid morbidity and mortality. the aim of this study was to evaluate the effect of overnight storage of aerobic and anaerobic bactec 9240 blood culture bottles on the detection time for common pathogens. | 2010 | 20457429 |
| emergence of resistant klebsiella pneumoniae in the intestinal tract during successful treatment of klebsiella pneumoniae lung infection in rats. | antibiotic treatment of lung infections may lead to the emergence of resistance in the gut flora. appropriate dosing regimens could mitigate this adverse effect. in gnotobiotic rats harboring intestinal escherichia coli and enterococcus faecium populations, a lung infection by klebsiella pneumoniae was instigated with two different sizes of inoculum to represent an early or a late initiation of antibiotic treatment. the rats were treated with marbofloxacin, an expanded-spectrum fluoroquinolone, ... | 2010 | 20457820 |
| lysozyme activates enterococcus faecium to induce necrotic cell death in macrophages. | enterococci are commensal organisms in the alimentary tract. however, they can cause a variety of life-threatening infections, especially in nosocomial settings. we hypothesized that induction of cell death might enable these facultative pathogenic bacteria to evade the innate immune response and to cause infections of their host. we demonstrate that e. faecium when exposed to lysozyme induces cell death in macrophages in vitro and in vivo. flow cytometric analyses of j774a.1 macrophages infecte ... | 2010 | 20458518 |
| specificity of induction of the vana and vanb operons in vancomycin-resistant enterococci by telavancin. | telavancin is a bactericidal, semisynthetic lipoglycopeptide indicated in the united states for the treatment of complicated skin and skin structure infections caused by susceptible gram-positive bacteria and is under investigation as a once-daily treatment for nosocomial pneumonia. the related vana and vanb gene clusters mediate acquired resistance to glycopeptides in enterococci by remodeling the dipeptide termini of peptidoglycan precursors from d-alanyl-d-alanine (d-ala-d-ala) to d-alanyl-d- ... | 2010 | 20404117 |
| influence of microfiltration and adjunct culture on quality of domiati cheese. | the effects of microfiltration and pasteurization processes on proteolysis, lipolysis, and flavor development in domiati cheese during 2 mo of pickling were studied. cultures of starter lactic acid bacteria isolated from egyptian dairy products were evaluated in experimental domiati cheese for flavor development capabilities. in the first trial, raw skim milk was microfiltered and then the protein:fat ratio was standardized using pasteurized cream. pasteurized milk with same protein:fat ratio wa ... | 2010 | 20412894 |
| vancomycin-resistant enterococci of vanb genotype may pose problems for screening with highly selective media. | 2010 | 20410340 | |
| peritonitis due to a peritoneal vaginal fistula. | development of a peritoneal vaginal fistula is rare and presents a unique clinical scenario, since the fistula wall is less likely to spontaneously heal because of the increased intra-abdominal pressure in the abdomen from the peritoneal fluid. catheter removal and surgical repair of the fistula are required. early catheter removal contributes to peritoneal membrane preservation. in the case of m.t., the most likely explanation was that the peritoneal fluid was leaking through the vaginal cuff c ... | 2010 | 20462078 |
| molecular epidemiology of vancomycin-resistant enterococcus faecium strains isolated from haematological malignancy patients in a research hospital in turkey. | infections and outbreaks of vancomycin-resistant enterococci (vre) still appear to be rare in turkey. in the present study, vre strains isolated during an outbreak in a haematology unit of a training and research hospital in turkey were typed and their antimicrobial-resistance patterns were characterized by molecular methods. twelve vancomycin-resistant enterococcus faecium strains isolated from patients with haematological malignancies were investigated by pcr for the presence of genes encoding ... | 2010 | 20223901 |
| enhancement of vancomycin activity by phenothiazines against vancomycin-resistant enterococcus faecium in vitro. | the antimicrobial and resistance-reversal activities of seven phenothiazine derivatives were evaluated against vancomycin-sensitive enterococcus faecalis atcc 29212, vancomycin resistant e. faecalis atcc 51299 and ten vancomycin-resistant e. faecium strains originating from human infections. minimum inhibitory concentrations (mic) of the compounds were determined by agar dilution method, and synergy between phenothiazines and vancomycin was investigated using checkerboard (microbroth dilution) t ... | 2010 | 20353486 |
| synthetic studies toward the mannopeptimycins: synthesis of orthogonally protected beta-hydroxyenduracididines. | the asymmetric synthesis of the nonproteinogenic amino acids (2s,3s,4's)-beta-hydroxyenduracididine 3 and (2r,3s,4's)-beta-hydroxyenduracididine 4 in orthogonally protected form in 15 total steps from garner's aldehyde is reported. the former and n-glycosylated form of the latter are found in the glycopeptide antibiotic mannopeptimycin. | 2010 | 20232818 |
| effect of fermented soy product on the fecal microbiota of rats fed on a beef-based animal diet. | the aim of this study was to determine whether the consumption of soy product fermented with enterococcus faecium crl 183, would modify the fecal microbiota of rats fed a diet containing red meat. the rats were placed in groups, distinguished by their diets. for 60 days, group i was given a standard casein-based rodent feed and groups ii-vi, the beef-based feed. from the 30th day, groups iii-vi also ingested the following products: group iii, e. faecium-fermented soy product; group iv, pure susp ... | 2010 | 20355036 |
| synthesis and antibacterial studies of binaphthyl-based tripeptoids. part 1. | an efficient synthesis of 29 new binaphthyl-based neutral, and mono- and di-cationic, peptoids is described. some of these compounds had antibacterial activities with mic values of 1.9-3.9microg/ml against staphylococcus aureus. one peptoid had a mic value of 6microg/ml against a methicillin-resistant strain of s. aureus (mrsa) and a mic value of 2microg/ml against vancomycin-resistant strains of enterococci (vre). | 2010 | 20236828 |
| removal of human enteric viruses and indicator microorganisms from domestic wastewater by aerated lagoons. | aerated lagoons offer a low-cost and simple approach to treating domestic wastewater in small municipalities. the objective of the current study was to evaluate, for each cell in the lagoons, the removal of indicator microorganisms and human enteric viruses under warm (summer) and cold (early spring) conditions. the two sites are located in southwest quebec, canada. samples were assayed for thermotolerant coliforms, enterococci, clostridium perfringens, somatic and male-specific coliphages, and ... | 2010 | 20237581 |
| biofilms in chronic bacterial prostatitis (nih-ii) and in prostatic calcifications. | the prevalence of inflammatory conditions of the prostate gland is increasing. in italy, there is a high incidence of prostatitis (13.3%), also accompanied by prostatic calcifications. cat nih-ii chronic bacterial prostatitis (cbps) are the most frequent. their aetiology theoretically involves the whole range of bacterial species that are able to form biofilms and infect prostate cells. the aim of our study was to isolate potential biofilm-producing bacteria from cbp patients, to evaluate their ... | 2010 | 20298500 |
| a review of the factors affecting sunlight inactivation of micro-organisms in waste stabilisation ponds: preliminary results for enterococci. | waste stabilisation ponds (wsp) are efficient, cost-effective methods of treating wastewater in rural and remote communities in australia. it is recognised that sunlight plays a significant role in their disinfection, however, due to the poor penetration of light in turbid waters it has been hypothesised that other mechanisms may also contribute to disinfection in wsps. to date, studies have reported various and conflicting results with regards to the relative contributions of uva, uvb, par and ... | 2010 | 20182066 |
| effect of chlorhexidine incorporation into a self-etching primer on dentine bond strength of a luting cement. | this study investigated the effect of incorporating chlorhexidine in a self-etching primer on the bond strength of an adhesive resin cement to dentine and on antibacterial activity. | 2010 | 20298738 |
| antimicrobial and antimalarial activity of cussonia species (araliaceae). | cussonia species are used in african traditional medicine mainly against pain, inflammation, gastro-intestinal problems, malaria and sexually transmitted diseases. | 2010 | 20298772 |
| risk factors for acquiring vancomycin-resistant enterococcus and methicillin-resistant staphylococcus aureus on a burn surgery step-down unit. | the incidence of hospital-associated infections secondary to methicillin-resistant staphylococcus aureus (mrsa) and those caused by vancomycin-resistant enterococci (vre) continue to increase, despite the publication of evidence-based guidelines on infection control. we sought to determine modifiable risks factors for acquisition of mrsa or vre or both on a burn trauma unit (btu). we performed a retrospective single-center-matched control study. our study group comprised 94 patients who acquired ... | 2010 | 20182380 |
| synergy of fosfomycin with other antibiotics for gram-positive and gram-negative bacteria. | the alarming increase in drug resistance and decreased production of new antibiotics necessitate the evaluation of combinations of existing antibiotics. fosfomycin shows no cross-resistance to other antibiotic classes. thus, its combination with other antibiotics may potentially show synergy against resistant bacteria. | 2010 | 20186407 |
| assessing pathogen risk to swimmers at non-sewage impacted recreational beaches. | the risk of gastrointestinal illness to swimmers from fresh sewage and non-sewage fecal sources at recreational beaches was predicted using quantitative microbial risk assessment (qmra). the qmra estimated the probability of illness for accidental ingestion of recreational water with a specific concentration of fecal indicator bacteria, here the geometric mean enterococci limit of 35 cfu 100 ml(-1), from either a mixture of sources or an individual source. using seagulls as an example non-sewage ... | 2010 | 20201509 |
| the extracytoplasmic function sigma factor sigv plays a key role in the original model of lysozyme resistance and virulence of enterococcus faecalis. | enterococcus faecalis is one of the leading agents of nosocomial infections. to cause diseases, pathogens or opportunistic bacteria have to adapt and survive to the defense systems encountered in the host. one of the most important compounds of the host innate defense response against invading microorganisms is lysozyme. it is found in a wide variety of body fluids, as well as in cells of the innate immune system. lysozyme could act either as a muramidase and/or as a cationic antimicrobial pepti ... | 2010 | 20300180 |
| enterococcus raffinosus sinusitis post-aspergillus flavus paranasal infection, in a patient with myelodysplastic syndrome: report of a case and concise review of pertinent literature. | a case of enterococcus raffinosus nosocomial sinusitis which appeared to complicate a previous aspergillus flavus paranasal infection is presented. this uncommon enterococcal species is rarely responsible for human diseases, and has never previously been associated with sinusitis. | 2010 | 20203228 |
| flow cytometry approach study of enterococcus faecalis vancomycin resistance by detection of vancomycin@fl binding to the bacterial cells. | we studied the usefulness of flow cytometry for detection of vancomycin resistance in enterococcus faecalis by direct binding of commercially available fluorescent vancomycin to cells obtained from culture. the cells were stained with vancomycin@fl, sonicated and additionally stained with propidium iodide (pi). regarding to inductive mechanism of vana-mediated vancomycin resistance, resistant reference strain was also pre-incubated with vancomycin. pi staining divided cells into two subpopulatio ... | 2010 | 20361190 |
| examination of equine glandular stomach lesions for bacteria, including helicobacter spp by fluorescence in situ hybridisation. | the equine glandular stomach is commonly affected by erosion and ulceration. the aim of this study was to assess whether bacteria, including helicobacter, could be involved in the aetiology of gastric glandular lesions seen in horses. | 2010 | 20298612 |
| culture-independent techniques applied to food industry water surveillance--a case study. | culture-independent techniques were used for the detection of pathogenic bacteria in drinking water at potentially critical control points along the production lines at a german dairy company and a spanish dry cured ham company. denaturing gradient gel electrophoresis (dgge) was used to describe bacterial population shifts indicating biological instability in the drinking water samples. autochthonous bacteria were identified by sequencing the excised dgge dna bands. more specifically, real-time ... | 2010 | 20363042 |
| surveillance and endemic vancomycin-resistant enterococci: some success in control is possible. | vancomycin-resistant enterococci (vre) are prevalent in many irish hospitals. we analysed surveillance data from 2001 to 2008 in a centre where vre is endemic. all clinically significant enterococci were tested for susceptibility to vancomycin. all intensive care unit admissions were screened on admission and weekly thereafter. interventions included isolating/cohorting vre patients, monthly prevalence surveys of vre patients, the introduction of an electronic alert system, programmes to improve ... | 2010 | 20363048 |
| microbial equol production attenuates colonic methanogenesis and sulphidogenesis in vitro. | hydrogen gas produced during colonic fermentation is excreted in breath and flatus, or removed by hydrogen-consuming bacteria such as methanogens and sulphate-reducing bacteria. however, recent research has shown that h2 is also consumed by equol-producing bacteria during the reduction of daidzein into equol. in this study, the interactions between methanogens, sulphate-reducing, and equol-producing bacteria were investigated under in vitro simulated intestinal conditions. in the presence of dai ... | 2010 | 20298796 |
| the necktie as a potential vector of infection: are doctors happy to do without? | 2010 | 20299125 | |
| transmission of nosocomial pathogens by white coats: an in-vitro model. | 2010 | 20299132 | |
| fatal vancomycin- and linezolid-resistant enterococcus faecium sepsis in a child undergoing allogeneic haematopoietic stem cell transplantation for beta-thalassaemia major. | recently vancomycin-resistant and sporadically linezolid-resistant enterococcus species have been described in adults. we report what we believe to be the first case of a child with prolonged bone marrow aplasia following haematopoietic stem cell transplantation developing a fatal sepsis caused by enterococcus faecium resistant to glycopeptides and linezolid. | 2010 | 20299507 |
| antibacterial effect of the bioactive compound beauvericin produced by fusarium proliferatum on solid medium of wheat. | to obtain the bioactive compound beauvericin (bea), fusarium proliferatum cect 20569 was grown on a solid medium of wheat, utilizing the technique of the solid state fermentation (ssf), being this mycotoxin purified by high performance liquid chromatography (hplc) with a reverse phase semi-preparative column using as the mobile phase acetonitrile/water in gradient condition. the purity of the bea was verified by analytical hplc and liquid chromatography tandem mass spectrometry (lc/ms-ms). the p ... | 2010 | 20371252 |
| enterococcal cerebellopontine angle abscess in a 12-year-old female. | despite advances in imaging and antibiotic treatment, brain abscess is still encountered occasionally. various aerobic and anaerobic bacteria have been reported as causative agents of brain abscess but only a few cases of enterococcal brain abscesses have been reported. here we report a case of brain abscess in a 12-year-old female patient, who presented with a history of fever, chills, headache, convulsions since seven days and history of altered sensorium and aphasia since the last two days. t ... | 2010 | 20300422 |
| harvested rainwater quality: the importance of appropriate design. | this paper summarises the physicochemical and microbiological quality of water from a rainwater harvesting (rwh) system in a uk-based office building. 7 microbiological and 34 physicochemical parameters were analysed during an 8 month period. physicochemically, harvested rainwater quality posed little health risk; most parameters showed concentrations below widely used guideline levels for drinking water. however, rwh system components (e.g. fittings and down pipes) appear to be affected soft wa ... | 2010 | 20371928 |
| causal connections between water quality and land use in a rural tropical island watershed: rural tropical island watershed analysis. | we examined associations between riparian canopy cover, presence or absence of cattle, rainfall, solar radiation, month of year, dissolved oxygen, turbidity, salinity, and enterococcus concentrations in riparian surface soils with enterococcus geometric mean in-stream water concentrations at waipā watershed on the north side of the hawaiian island kaua'i. each 1% decrease in riparian canopy cover was associated with a 4.6 most probable number (mpn)/100 ml increase of the geometric mean of entero ... | 2010 | 20300800 |
| combined effect of enterocin as-48 and high hydrostatic pressure to control food-borne pathogens inoculated in low acid fermented sausages. | the single and combined effects of enterocin as-48 and high hydrostatic pressure (hhp) on listeria monocytogenes, salmonellaenterica, and staphylococcus aureus was investigated in fuet (a low acid fermented sausage) during ripening and storage at 7 degrees c or at room temperature. as-48 (148 au g(-1)) caused a drastic 5.5 log cfu g(-1) decrease in l. monocytogenes (p<0.001) and a significant (p<0.01) inhibition (1.79 logs) for salmonella at the end of ripening (10 d). after pressurization (400 ... | 2010 | 20374829 |
| phenotypic and genotypic characterization of lactic acid bacteria isolated from artisanal italian goat cheese. | the lactic acid bacteria community in traditional goat cheese produced in three dairies in valsesia (piemonte, italy) was studied at different steps of the manufacturing process. these cheeses were produced from raw milk without starter bacteria, and no protocol was followed during the manufacturing process. three hundred thirty-two isolates were characterized and grouped by results of both morphophysiological tests and random amplification of polymorphic dna plus pcr analysis. bacteria were ide ... | 2010 | 20377953 |
| expression of the avian-specific toll-like receptor 15 in chicken heterophils is mediated by gram-negative and gram-positive bacteria, but not tlr agonists. | toll-like receptors (tlrs) are a critical component of the innate immune response of mammalian and avian species. while most mammalian tlrs have been well characterized, the chicken-specific tlr15 has not been extensively studied. we recently demonstrated that tlr15 is differentially expressed between salmonella-susceptible-and-resistant chickens, indicating a potential role in the innate immune response to infection with salmonella. the aim of the present study was to gain better insight into t ... | 2010 | 20303182 |
| [study of the first outbreak of vana enterococcus faecium in the canary islands]. | in july, 2005 the first vancomycin-resistant enterococcus faecium (vref) with a genotype vana was isolated in hospital universitario de canarias (huc). from september to december 2005, vref vana was isolated from another 15 patients (3 nosocomial infections and 12 rectal carriers). all of them were kidney transplant patients hospitalized in the nephrology ward. this study describes the first vref vana outbreak in the huc and the epidemiological link of the first vref vana isolates found in anoth ... | 2010 | 20378209 |
| effect of inoculated corn silage enriched with sunflower oil on rumen fermentation and lipid metabolism in an artificial rumen (rusitec). | some rumen isolates are able to produce conjugated linoleic acid (cla) from linoleic acid (la) in vitro. effects of providing diets containing corn silage (cs) and lucerne hay to an artificial rumen (rusitec) in which the corn was not inoculated (cs), or inoculated with lactobacillus plantarum ccm 4000 (cs + lp), lactobacillus fermentum lf2 (cs + lf) or enterococcus faecium ccm 4231 (cs + ef) and supplied with sunflower oil (so; 30 g kg(-1); w/w) on rumen metabolism were examined. | 2010 | 20355015 |
| evaluation of efflux activity of bacteria by a semi-automated fluorometric system. | a semi-automated method that uses the common efflux pump (ep) substrate ethidium bromide (eb) is described for the assessment of ep systems of bacteria. the method employs the rotor-gene(tm) 3000 thermocycler (corbett research) for the real-time assessment of accumulation and efflux of eb in phosphate-buffered solution (pbs) under varying physiological conditions, such as temperature, ph, presence and absence of the energy source, and presence of efflux pumps inhibitors (epis). the method is suf ... | 2010 | 20401593 |
| the probiotic paradox: live and dead cells are biological response modifiers. | probiotics are usually defined as products which contain viable non-pathogenic micro-organisms able to confer health benefits to the host. there are specific gastrointestinal effects of probiotics such as alleviating inflammatory bowel disease, reducing acute diarrhoea in children, inhibiting salmonella and helicobacter pylori, removing cholesterol, secreting enzymes and bacteriocins and immunomodulation. however, many of the effects obtained from viable cells of probiotics are also obtained fro ... | 2010 | 20403231 |
| characterization of enterococcus spp. from human and animal feces using 16s rrna sequences, the esp gene, and pfge for microbial source tracking in korea. | contamination from human and animal fecal waste is a primary cause of water pollution. microbial source tracking (mst) may be a useful tool for high-quality environmental management and for assessing human health risks associated with water pollution. the goal of this study was to evaluate enterococcus spp. as a target organism for mst. thirty-four fecal samples were collected from five different sources (human, chicken, pig, cow, and goose) in south korea. in total, 237 enterococcus spp. were i ... | 2010 | 20356091 |
| effects of probiotics on acquisition and spread of multiresistant enterococci. | ampicillin-resistant enterococcus faecium (are) and vancomycin-resistant e. faecium (vre) are important nosocomial pathogens. we quantified effects of probiotics and antibiotics on intestinal acquisition of are colonization in patients hospitalized in two non-intensive care unit (non-icu) wards with high are prevalence. in a prospective cohort study with crossover design, all patients with a length of stay of >48 h were offered a multispecies probiotic product twice daily until discharge (4.5 mo ... | 2010 | 20404120 |
| synthesis of an alpha-kojibiosyl substituted glycerol teichoic acid hexamer. | in this paper the synthesis of an enterococcus faecalis teichoic acid (ta) hexamer is presented. the key kojibiosyl-glycerol phosphoramidite building block was obtained by condensation of thioglucose donors, provided with various protecting groups at the c2 hydroxyl function with an orthogonally protected glycerol acceptor. after selective deprotection, the resulting 1,2-cis-linked pseudodisaccharide acceptor was coupled to an alpha-directing thioglucose donor, giving the corresponding pseudotri ... | 2010 | 20409722 |
| emergence of vanb enterococcus gallinarum colonization in association with glycopeptide therapy. | 2010 | 20357217 | |
| spectrum and prevalence of fungi infecting deep tissues of lower-limb wounds in patients with type 2 diabetes. | the prevalence rate and spectrum of fungi infecting deep tissues of diabetic lower-limb wounds (dlws) have not been previously studied. five hundred eighteen (382 male and 136 female) consecutive patients with type 2 diabetes hospitalized due to infected lower-limb wounds were enlisted in this study. deep tissue (approximately 0.5- x 0.5-cm size) taken perioperatively from the wound bed was cultured for fungi. fungi was found in 27.2% (141/518) of the study population. candida parapsilosis (25.5 ... | 2010 | 20410345 |
| isolation, phylogenetic analysis and anti-infective activity screening of marine sponge-associated actinomycetes. | terrestrial actinomycetes are noteworthy producers of a multitude of antibiotics, however the marine representatives are much less studied in this regard. in this study, 90 actinomycetes were isolated from 11 different species of marine sponges that had been collected from offshore ras mohamed (egypt) and from rovinj (croatia). phylogenetic characterization of the isolates based on 16s rrna gene sequencing supported their assignment to 18 different actinomycete genera representing seven differen ... | 2010 | 20411105 |
| [infectious endocarditis]. | 2010 | 20358500 | |
| initial development and structure of biofilms on microbial fuel cell anodes. | microbial fuel cells (mfcs) rely on electrochemically active bacteria to capture the chemical energy contained in organics and convert it to electrical energy. bacteria develop biofilms on the mfc electrodes, allowing considerable conversion capacity and opportunities for extracellular electron transfer (eet). the present knowledge on eet is centred around two gram-negative models, i.e. shewanella and geobacter species, as it is believed that gram-positives cannot perform eet by themselves as th ... | 2010 | 20356407 |
| identification of the bacterial biodiversity in koumiss by denaturing gradient gel electrophoresis and species-specific polymerase chain reaction. | bacterial biodiversity in traditional koumiss fermented milk was studied by denaturing gradient gel electrophoresis (dgge). target dna bands were identified according to the reference species ladder, constructed in this study. comigrating bands present in the dgge profiles were resolved by species-specific pcr. the results revealed a novel bacterial profile and extensive bacterial biodiversity in koumiss. the dominant lactic acid bacteria included lactobacillus acidophilus, lactobacillus helveti ... | 2010 | 20412906 |
| recovery and screening for antibiotic susceptibility of potential bacterial pathogens from the oral cavity of shark species involved in attacks on humans in recife, brazil. | the number of incidents involving sharks and humans at beaches in recife, on the north-eastern brazilian coast, is among the highest worldwide. in addition, wound infections in survivors are common; but the nature and risk of the aetiological agents is unknown. in the present study, 81 potential bacterial pathogens were identified in the oral cavity of sharks involved in attacks in recife, and were subjected to antibiotic susceptibility tests using the standardized disc-diffusion method. the maj ... | 2010 | 20413619 |
| [characteristics of urinary tract infections in different patient subpopulations and depending on the bladder emptying system]. | to analyze the prevalence and etiology of urinary tract infection in patients with neurogenic bladder depending on the bladder emptying system used as compared to a population with no neurological impairment. to assess perception of urinary tract infection by patients with neurogenic bladder. | 2010 | 20416242 |
| immunogenic potential of enterococcus faecalis biofilm under simulated growth conditions. | the aim of this study was to evaluate the effect of growth modes (planktonic and biofilms) of enterococcus faecalis on the intracellular survival and ability to produce tumor necrosis factor alpha (tnf-alpha) and interleukin (il)-6 after interacting with monocytes/in vitro-differentiated macrophages. | 2010 | 20416428 |
| effect of ethylenediaminetetraacetic acid and sodium hypochlorite irrigation on enterococcus faecalis biofilm colonization in young and old human root canal dentin: in vitro study. | the alterations in dentin tissue depending on increasing age might cause different adhesion capability of bacteria, yielding differences in clinical approaches regarding root canal irrigation. this study, therefore, aimed to evaluate the effects of ethylenediaminetetraacetic acid (edta) and sodium hypochlorite (naocl) on enterococcus faecalis biofilm growth in root canal dentin of young and old individuals. | 2010 | 20416430 |
| effectiveness of chemomechanical preparation with alternating use of sodium hypochlorite and edta in eliminating intracanal enterococcus faecalis biofilm. | the elimination of microorganisms from root canals is a critical step in endodontic treatment. we aimed to evaluate the antimicrobial effectiveness of an alternating irrigation regimen during chemomechanical preparation (cmp). | 2010 | 20416441 |
| bacterial leakage in obturated root canals-part 2: a comparative histologic and microbiologic analyses. | in this study, presence of dentin infection in root canals, obturated with 4 techniques submitted to the bacterial leakage test, was evaluated using histologic methods. | 2010 | 20416539 |
| detoxification of aflatoxin b1 and patulin by enterococcus faecium strains. | aim of the present study was to investigate the detoxification of aflatoxin b(1) and patulin from aqueous solution by probiotic culture of enterococcus faecium m74 and commercial culture of e. faecium ef031. the effect of the bacterial viability, incubation time and ph of the medium on the binding ability was tested. also, binding stability was determined by washing the bacteria-mycotoxin complexes with phosphate buffer saline. both m74 and ef031 strains have the ability to remove aflatoxin b(1) ... | 2010 | 20356644 |
| effect of enterococcus faecium al41 and thymus vulgaris essential oil on small intestine integrity and antioxidative status of laying hens. | we investigated the effect of enterococcus faecium on phagocytic activity, antioxidative status in vivo and the effect of e. faecium and 0.4% concentration of thymus vulgaris essential oil (eo) on the duodenal tissue integrity in vitro in laying hens. the birds were fed the same standard diets and were divided into four groups. e. faecium was added to the drinking water for the second and fourth groups. eo was added to special chambers for measuring trans-epithelial electrical resistance (teer) ... | 2010 | 20362314 |
| a potential role for daptomycin in enterococcal infections: what is the evidence? | nosocomial infections caused by enterococci present a challenge for clinicians because treatment options are often limited due to the widespread occurrence of strains resistant to multiple antibiotics, including vancomycin. daptomycin is a first-in-class cyclic lipopeptide that has proven efficacy for the treatment of gram-positive infections. although methicillin-resistant staphylococcus aureus has been the most prominent target in the clinical development of daptomycin, this agent has demonstr ... | 2010 | 20363805 |
| emergence of high ampicillin-resistant enterococcus faecium isolates in a kidney transplant ward: role of antibiotic pressure and cross transmission. | the epidemiology of patients associated with ampicillin-resistant enterococcus faecium (are) was investigated by combining both clinical approach and molecular analysis in a kidney transplant patient's ward. a case-control study was performed to identify risk factors for are by matching each patient with are with two control patients without any isolated e. faecium strain. are isolates were characterized by pulsed-field gel electrophoresis. from june 2004 to may 2006, 18 cases with clinical are ... | 2010 | 20370509 |
| antimicrobial resistance and in vitro biofilm-forming ability of enterococci from intensive and extensive farming broilers. | enterococci, major broiler intestinal colonizers, play a recognized role in antimicrobial resistance transmission. several virulence mechanisms, such as biofilm expression, have been identified. minimum inhibitory concentrations of vancomycin, enrofloxacin, oxytetracycline, streptomycin, and gentamicin and biofilm production of 34 isolates from intensive and extensive farming system broilers were evaluated. all isolates were susceptible to vancomycin. in extensive-reared broilers (n = 18), resis ... | 2010 | 20371861 |
| photocatalytic inactivation of e. faecalis in secondary wastewater plant effluents. | photocatalytic inactivation of enterococcus faecalis using tio(2) suspensions was investigated and compared to the inactivation of the most commonly used faecal indicator strain escherichia coli. in contrast to the inactivation in pure deionized water, disinfection of effluents from the biological process of an urban wastewater plant showed a longer initial lag phase and higher survival fractions after several hours of irradiation. moreover, the fluctuation of the composition of the effluents st ... | 2010 | 20418633 |
| [hybrisep, a new method for the diagnosis of blood culture-negative infective endocarditis: a case report]. | blood cultures were considered as the gold standard for the diagnosis of infective endocarditis (ie) but were frequently negative. this report describes an ie patient who successfully underwent aortic valve replacement; it discusses a new diagnostic method of blood culture-negative ie. recently, hybrisep (fuso pharmaceutical industries, japan), a rapid diagnostic method for bacteremia was developed using in situ hybridization, which is superior in sensitivity to conventional blood cultures. in t ... | 2010 | 20420142 |
| characterization of bacteriocins produced by two strains of lactobacillus plantarum isolated from beloura and chouriço, traditional pork products from portugal. | bacteriocins bacst202ch and bacst216ch, produced by lactobacillus plantarum strains isolated from beloura and chouriço, respectively, inhibited the growth of a number of gram-positive and gram-negative meat spoilage bacteria. according to trycine-sds-page, bacst202ch and bacst216ch are approximately 3.5 and 10.0 kda in size, respectively. maximal activity of bacst202ch (25,600 au/ml) was recorded after 27 h of and bacst216ch (102,400 au/ml) after 22 h of growth. the mode of activity, as determin ... | 2010 | 20374794 |
| peroxisome proliferator-activated receptor gamma activation is required for maintenance of innate antimicrobial immunity in the colon. | crohn's disease (cd), a major form of human inflammatory bowel disease, is characterized by primary immunodeficiencies. the nuclear receptor peroxisome proliferator-activated receptor gamma (ppargamma) is essential for intestinal homeostasis in response to both dietary- and microbiota-derived signals. its role in host defense remains unknown, however. we show that ppargamma functions as an antimicrobial factor by maintaining constitutive epithelial expression of a subset of beta-defensin in the ... | 2010 | 20421464 |
| biosynthesis of peptide signals in gram-positive bacteria. | gram-positive bacteria coordinate social behavior by sensing the extracellular level of peptide signals. these signals are biosynthesized through divergent pathways and some possess unusual functional chemistry as a result of posttranslational modifications. in this chapter, the biosynthetic pathways of bacillus intracellular signaling peptides, enterococcus pheromones, bacillus subtilis competence pheromones, and cyclic peptide signals from staphylococcus and other bacteria are covered. with th ... | 2010 | 20378052 |
| genome-wide rna interference in drosophila cells identifies g protein-coupled receptor kinase 2 as a conserved regulator of nf-kappab signaling. | because nf-kappab signaling pathways are highly conserved in evolution, the fruit fly drosophila melanogaster provides a good model to study these cascades. we carried out an rna interference (rnai)-based genome-wide in vitro reporter assay screen in drosophila for components of nf-kappab pathways. we analyzed 16,025 dsrna-treatments and identified 10 novel nf-kappab regulators. of these, nine dsrna-treatments affect primarily the toll pathway. g protein-coupled receptor kinase (gprk)2, cg15737/ ... | 2010 | 20421637 |
| [effectiveness and risks of isolation precautions in patients with mrsa and other multidrug-resistant bacteria]. | the transmission of multidrug-resistant organisms (mrsa, vre and esbl producing bacteria) occurs predominantly if health-care workers are not compliant with hand hygiene procedures. the impact of single-room isolation in transmission prevention is often overestimated. as long as hand disinfection is not performed before and after patient contact and gloves are not removed, a single room will not prevent transmission by -itself. understaffing is additionally worsening the situation. there is no c ... | 2010 | 20379942 |
| estimation of enterococci input from bathers and animals on a recreational beach using camera images. | enterococci, are used nationwide as a water quality indicator of marine recreational beaches. prior research has demonstrated that enterococci inputs to the study beach site (located in miami, fl) are dominated by non-point sources (including humans and animals). we have estimated their respective source functions by developing a counting methodology for individuals to better understand their non-point source load impacts. the method utilizes camera images of the beach taken at regular time inte ... | 2010 | 20381094 |
| [linezolid therapy for infectious endocarditis]. | in denmark enterococci causes 15 to 20% of all endocarditis (ie) cases. the development of multi-resistant bacterial strains has increased the need for new antibiotics. linezolid is an alternative to conventional treatment of infections with gram positive cocci. in this case report linezolid was used to treat ie in a patient, who was allergic to penicillin and where conventional treatment caused development of acute renal failure. no side effects were observed and the patient responded well to t ... | 2010 | 20423667 |
| antibiotic resistance in community-acquired urinary tract infections: prevalence and risk factors. | this study aimed to identify the agents causing community-acquired urinary tract infections (cautis) and their resistance patterns and to investigate risk factors for esbl production. | 2010 | 20424552 |
| isolation of bacteria from mouse caecal samples and description of bacteroides sartorii sp. nov. | caecal samples from wild-type and tnf(deltaare) mice were cultured on selective media containing bile salts, amino acids or casein macro-peptides. twenty-two strains were isolated and identified by 16s rrna gene sequencing. twenty-one strains showed >98% similarity to known bacteria (blautia spp., clostridium innocuum, enterococcus spp., escherichia coli, lactobacillus murinus, parabacteroides goldsteinii and shigella dysenteriae). one additional isolate, strain a-c2-0, was a new bacterium. the ... | 2010 | 20369227 |
| antibacterial effect of bioactive glass in combination with powdered enamel and dentin. | in endodontics, various intracanal medications have been advocated to eliminate bacteria after root canal instrumentation. a recent study has revealed that addition of powdered dentin to bioactive glass (bag) led to increased glass dissolution, and an increased antibacterial efficacy. therefore, the present study was undertaken to compare the effect of enamel and dentin powder on the antibacterial efficacy of a commercially available bag. | 2010 | 20427903 |
| endodontic sealers: intratubular penetration and permeability to enterococcus faecalis. | evaluate in vitro the intratubular penetration and permeability of endodontic sealers in teeth contaminated with enterococcus faecalis. | 2010 | 20427905 |
| gold(i) complexes of n-heterocyclic carbene ligands containing benzimidazole: synthesis and antimicrobial activity. | gold(i) n-heterocyclic carbene (nhc) complexes were obtained in good yields from the corresponding silver complexes by treatment with [aucl(pph3)] following the commonly used silver carbene transfer route. the silver complexes were synthesized from the benzimidazolium halide salts by the in situ reactions with ag2o in dichloromethane as a solvent at room temperature. all gold complexes have been characterized by 1h-nmr, 13c-nmr and ir spectroscopy and elemental analysis. au-nhc complexes were ev ... | 2010 | 20428038 |
| synthesis and antimicrobial activity of novel ag-n-hetero-cyclic carbene complexes. | a series of imidazolidinium ligand precursors are metallated with ag2o to give silver(i) n-heterocyclic carbene complexes. all compounds were fully characterized by elemental analyses, 1h-nmr, 13c-nmr and ir spectroscopy techniques. all compounds studied in this work were screened for their in vitro antimicrobial activities against the standard strains: enterococcus faecalis (atcc 29212), staphylococcus aureus (atcc 29213), escherichia coli (atcc 25922), pseudomonas aeruginosa (atcc 27853) and t ... | 2010 | 20428059 |
| a genetic determinant in streptococcus gordonii challis encodes a peptide with activity similar to that of enterococcal sex pheromone cam373, which facilitates intergeneric dna transfer. | enterococcus faecalis strains secrete multiple peptides representing different sex pheromones that induce mating responses by bacteria carrying specific conjugative plasmids. the pheromone cam373, which induces a response by the enterococcal plasmid pam373, has been of interest because a similar activity is also secreted by streptococcus gordonii and staphylococcus aureus. the potential to facilitate intergeneric dna transfer from e. faecalis is of concern because of extensive multiple antibioti ... | 2010 | 20233933 |
| probiotic treatment of irritable bowel syndrome in children. | treatment of functional bowel disorders of irritable bowel-type (ibs) in children remains a difficult task because of a lack of drugs with low adverse event profile. we here report the results of a treatment study in 203 children (66 boys and 137 girls) age 4 to 18 years (mean: 10.5+/-4.5 years) with typical ibs symptoms with abdominal pain and either predominant diarrhea (n=50), constipation (n=56), alternating stool frequency (n=28) or unspecific pain (n=69). the average duration of symptoms p ... | 2010 | 20234804 |
| pediatric urinary tract infections in a tertiary care center from north india. | paediatric urinary tract infections (uti) are associated with high morbidity and long term complications like renal scarring, hypertension, and chronic renal failure. a cause of occult febrile illness, they often remain undiagnosed. we studied the clinical and microbiologic profile and antibiotic resistance profile of such infections in paediatric uti patients at our center. | 2010 | 20167982 |
| recent increase in enterococci, viridans streptococci, pseudomonas spp. and multiresistant strains among haematological patients, with a negative impact on outcome. results of a 3-year surveillance study at a single institution. | we prospectively analysed the microbiological isolates of all febrile/infectious episodes occurring at our haematology unit during 2 consecutive 18-month periods. microbiologically documented infections (mdi) and antibiotic resistance were correlated with type and status of haematological disease, neutropenia, levofloxacin prophylaxis, central venous catheter and clinical outcome. three hundred and ten mdi were observed and 369 pathogens were isolated. gram-negative bacteria represented 49.3% an ... | 2010 | 20100118 |
| natural orifice transluminal endoscopic surgery versus laparoscopic surgery for inadvertent colon injury repair: feasibility, risk of abdominal adhesions, and peritoneal contamination in a porcine survival model. | adhesions are common after conventional surgery; natural orifice transluminal endoscopic surgery (notes) avoids peritoneal disruption and may reduce adhesions. | 2010 | 20170909 |
| detection of enterococcus faecalis in subgingival biofilm of patients with chronic refractory periodontitis. | refractory periodontitis is the occurrence of additional clinical attachment loss after repeated attempts to control the infection with conventional periodontal therapy. some microorganisms seem to be involved in the pathogenesis of chronic refractory periodontitis. the prevalence of enterococcus faecalis in the oral cavity seems to be higher in individuals with periodontitis. therefore, the present study investigated the presence of e. faecalis in subgingival biofilm of patients with chronic re ... | 2010 | 20173722 |
| the changing face of prosthetic valve endocarditis at a tertiary-care hospital: 1986-2005. | to investigate changes in the clinical characteristics, etiology and prognosis of prosthetic valve endocarditis at a tertiary-care hospital. | 2010 | 20089223 |
| microbiological outcome of complicated urinary tract infections treated with levofloxacin: a pharmacokinetic/pharmacodynamic analysis. | the pharmacodynamic targets representing 90% probability thresholds for bacterial eradication were determined in patients with complicated urinary tract infections (utis) treated with 500mg of levofloxacin every 24h for 7-14 days. of 241 pre-treatment strains from 156 patients, 21 strains persisted after treatment. in each patient, plasma concentrations of levofloxacin were simulated based on population pharmacokinetic parameters and patient-specific data. minimum inhibitory concentrations (mics ... | 2010 | 20226636 |