Publications

organisms isolated from dogs and cats with anaerobic infections and susceptibility to selected antimicrobial agents.	to determine the prevalence of obligate anaerobic bacteria in bacterial infections in dogs and cats and susceptibility to selected antimicrobial agents.	1997	9170087
protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum.	the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ...	1997	9178115
periodontal findings in cohen syndrome with chronic neutropenia.	radiographic periodontal status and microbiological findings of periodontal pockets in subjects with cohen syndrome are presented in this report. this hereditary disorder causes mental retardation, and neutropenia is one feature of the syndrome. fifteen patients with cohen syndrome and 15 controls matched for age and sex and, as far as possible, according to the degree of mental retardation were examined. alveolar bone loss was evaluated from the panoramic radiographs. two subgingival samples we ...	1997	9182743
microbiology of nonbullous impetigo.	our objective was to establish the aerobic and anaerobic microbiology of nonbullous impetigo (ni) in children. we used a retrospective review of clinical microbiology laboratory and patients' records. specimens were obtained from 40 patients with ni lesions and showed bacterial growth. aerobic or facultative anaerobic bacteria only were present in 24 patients (60%), strict anaerobic bacteria only in 5 patients (12.5%), and mixed anaerobic-aerobic flora was present in 11 patients (27.5%). sixty-f ...	1997	9192410
[meropenem: microbiologic perspective].	meropenem is a beta-lactamic carbapenem derived from thienamycin and is structurally characterized by the presence of a beta-methyl group in position c1 which confers stability to the molecule versus renal dehydropeptidase 1 (dhp-1), thereby making the coadministration of an enzyme inhibitor unnecessary. its esterochemical configuration of the lateral chain in c2 (dimethyl carbomoilpyrrolidenethium) increases the activity versus gram negative bacteria (enterobacteria and pseudomonas) and moreove ...	1997	9410064
[anti-anaerobic activity of carbapenems].	the critical concentrations of sensitivity and resistance for meropenem versus anaerobic bacterias were analyzed. it is demonstrated the meropenem is a powerful inhibitor of the microorganisms of the bacteroides groups (mic90 < 1 mg/l), prevotella > porphyromonas, fusobacterium, veillonella, clostridium perfringens is inhibited at a mic < 0.06 mg/l. the mic of meropenem versus c. difficile is 2 mg/l. they are also highly susceptible to the propionibacterium, peptostreptococcus and peptococcus ty ...	1997	9410076
[aerobic and anaerobic bacterial flora in chronic sinusitis in adults].	the aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). the isolated aerobic bacteria were as follows: streptococci from the species streptococcus salivarius, streptococcus anginosus, streptococcus group c, streptococcus sanguis, ...	1997	9411078
[microflora of periodontal pockets in advanced periodontitis].	the aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. from each subject 16-20 samples were taken using paper points. pooled sample after 60 s. mixing was serially diluted in reduced bhi. for total cell counts and for the isolation of black pigmented anaerobes brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without kanamycin-vancomycin mixture and bm agar plates were used. for isolation of a. actinomycetemcomitans ...	1997	9411079
contrasting roles for symmetrically disposed beta-turns in the folding of a small protein.	to investigate the role of turns in protein folding, we have characterized the effects of combinatorial and site-directed mutations in the two beta-turns of peptostreptococcal protein l on folding thermodynamics and kinetics. sequences of folded variants recovered from combinatorial libraries using a phase display selection method were considerably more variable in the second turn than in the first turn. these combinatorial mutants as well as strategically placed point mutants in the two turns h ...	1997	9417937
a microbiological study of pre- and postoperative apicoectomy sites.	there is little information on the microbiology of periapical lesions, and no data on the residual microbial flora in the periapex, if any, after apicoectomy procedures. hence, 64 patients treated by apicoectomy procedures were prospectively studied to assess the bacterial flora in the periapex and to evaluate the residual bacteria in postoperative apicoectomy sites. of the 64 lesions studied, 14 (22%) were sterile and 50 (78%) yielded bacteria preoperatively. bacteria could be recovered from 28 ...	1997	9552822
secondary bacterial infection in ghanaian patients with scabies.	from 110 patients with secondarily infected scabies lesions, 105 bacteria consisting of 66 aerobes and 39 anaerobes were isolated. a mixture of aerobic and anaerobic bacteria was present in 15 (13.6%). the predominant aerobic and anaerobic bacteria were staphylococcous aureus 39.1% and pepostreptococcus spp. 14.2% respectively. organisms that resided in the mucus membranes close to or in contact with the lesions predominated in those infections. most organisms were recovered from the finger and ...	1997	9557448
[non-sporeforming anaerobic bacteria in bronchoalveolar lavage fluid of patients with pneumonia during the course of lung cancer].	the aim of this study was to assess the occurrence of non-sporeforming anaerobes in bronchoalveolar lavage in patients with pneumonia coexisting with lung cancer. material consisted of 40 patients with lung cancer. bronchoalveolar lavage was performed before administration of antimicrobial treatment and obtained material was cultivated in anaerobic and aerobic conditions. quantitative assessment of anaerobic bacteria and their susceptibility to common antimicrobial agents were also performed. pe ...	1997	9489438
periapical abscesses: causal bacteria and antibiotic sensitivity.	the purpose of this study was to investigate the aerobic and anaerobic flora of periapical abscesses and evaluate their susceptibility to various antibiotics. in 52 patients, with a diagnosis of periapical abscesses, pus cultures were taken. forty-two aerobes and 122 anaerobes were revealed, with 2 or more than 2 anaerobic strains isolated in 36 patients. infections were purely aerobic in 6%, purely anaerobic in 17% and mixed in 75%, while in 2% of the specimens there was no growth of microorgan ...	1997	9491841
aerobic, anaerobic and fungal burn wound infections.	one hundred and twenty-seven patients were studied prospectively for aerobic, anaerobic and fungal burn wound infections. all cases yielded organisms on culture. a total of 377 isolates were recovered (239 aerobes, 116 anaerobes and 22 fungi). aerobic bacteria alone were present in 49 patients (38.6%). anaerobic bacteria alone were present in four patients (3.2%). candida sp. alone was present in one patient (0.8%). mixed aerobic and/or anaerobic bacteria and/or fungi were present in 73 patients ...	1997	9457609
periodontitis patient and the spouse: periodontal bacteria before and after treatment.	suspected periodontal pathogens can be transmitted between spouses. the treatment response may be unsuccessful in periodontitis patients, if the spouse harbors these bacteria. the aim of the present 6-month follow-up study was to clarify whether the microbiological treatment outcome of periodontitis patients is related to the detection of suspected periodontal pathogens in the saliva of the spouse. 10 patients with advanced periodontitis and their spouses were included in the study. the patients ...	1997	9442426
chronic sinusitis in children.	in children, sinusitis is a common, generally uncomplicated, and self limiting disease which drops with age. the diagnosis is difficult because of relatively non specific signs and symptoms that overlap with viral upper respiratory infection and allergy. plain paranasal sinus radiographs are not adequate, in determining the extent of involvement in recurrent or chronic sinusitis and so ct scan has become the standard. viral illness appears to be the most common predisposing factor. immune defect ...	1997	9444376
effect of 2-bromoethanesulfonic acid and peptostreptococcus productus atcc 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis.	evidence is provided that reductive acetogenesis can be stimulated in ruminal samples during short-term (24-h) incubations when methanogenesis is inhibited selectively. while addition of the reductive acetogen peptostreptococcus productus atcc 35244 alone had no significant influence on ch4 and volatile fatty acid (vfa) production in ruminal samples, the addition of this strain together with 2-bromoethanesulfonic acid (bes) (final concentration, 0.01 or 0.03 mm) resulted in stimulation of acetic ...	1997	8979351
[a case of subacute bacterial endocarditis caused by anaerobic peptostreptococcus].		1997	9868074
[a study of anaerobic infection in maxillofacial region].	to analyse the anaerobic infection of maxillofacial surgery and estimate the efficacy of antianaerobic therapy, 45 patients were divided into two groups, tinidazole group and metronidazole group. bacterial culture was positive before treating in all cases. there were bacillus melaniogenicus, veillonella, peptococcus and peptostreptococus, etc. there was excellent efficacy in the treatment of maxillofacial anaerobic infection by tinidazole intravenously. after treatment, the result of bacterial e ...	1997	9868121
conformational analysis of peptides corresponding to all the secondary structure elements of protein l b1 domain: secondary structure propensities are not conserved in proteins with the same fold.	the solution conformation of three peptides corresponding to the two beta-hairpins and the alpha-helix of the protein l b1 domain have been analyzed by circular dichroism (cd) and nuclear magnetic resonance spectroscopy (nmr). in aqueous solution, the three peptides show low populations of native and non-native locally folded structures, but no well-defined hairpin or helix structures are formed. in 30% aqueous trifluoroethanol (tfe), the peptide corresponding to the alpha-helix adopts a high po ...	1997	9007989
contemporary management of deep neck space infections.	deep neck infections continue to be seen despite the wide use of antibiotics. these infections follow along fascial planes to create deep neck space abscesses. the clinical presentation often points to the space involved. understanding the regional anatomy gives the surgeon the ability to treat these grave infections. the records of 24 patients with a diagnosis of deep neck space abscess admitted to hermann hospital between 1988 and 1993 were reviewed. fifty percent of the patients had received ...	1997	9018251
microbiology of otitis externa.	microbiologic and clinical data from 26 patients with otitis externa were prospectively evaluated. specimens were processed for aerobic and anaerobic bacteria. bacterial growth was noted in 23 specimens. a total of 33 aerobic and 2 anaerobic bacteria were recovered. aerobic bacteria only were isolated in 21 (91%) patients, anaerobic bacteria only in 1 (4%), and mixed aerobic and anaerobic bacteria in 1 (4%). the most common isolates were pseudomonas aeruginosa (14 instances), staphylococcus aure ...	1997	9018252
evaluation of the anaeropack system for growth of clinically significant anaerobes.	the anaeropack (mitsubishi gas chemical america, inc., new york, n.y.) system was compared with the gaspak (becton dickinson microbiology systems, cockeysville, md.) system and a conventional anaerobe chamber to evaluate the ability of the anaeropack system to support the growth of clinically significant anaerobes. the anaeropack system requires no catalyst or water, produces no hydrogen, and is oxygen absorbing and carbon dioxide generating. it is simple to use and reduces preparation time to a ...	1997	9041388
the clinical, microbial, and host response characteristics of the failing implant.	the goal of this study was to provide new data regarding levels of inflammatory and growth factor mediators and bacterial pathogens associated with failing implants, as compared to healthy implants. twenty-one patients with failing implant sites (group 1) and 8 patients with only healthy implants (group 2) were included. fifteen of the 21 failing implant patients (group 1) also presented with at least one stable nondiseased implant. plaque samples were examined, using dna oligonucleotide probes ...	1997	9048452
equilibrium and pre-equilibrium fluorescence studies on the interaction between protein l and kappa light chain.		1997	9056936
aerobic and anaerobic microbiology of bacterial tracheitis in children.	establish the aerobic and anaerobic microbiology of bacterial tracheitis in children.	1997	9061728
inhibitory effects of metronidazole on anaerobic metabolism of phenylalanine and leucine by peptostreptococcus anaerobius.	the metabolism of phenylalanine and leucine by peptostreptococcus anaerobius and the effects of metronidazole on this metabolism were studied. the cells metabolized both phenylalanine and leucine under strict anaerobic conditions and produced hydroxylated products (i.e. phenyllactate from phenylalanine and hydroxyisocaproate from leucine). other products were phenylpropionate, cinnamate and phenylacetate from phenylalanine; isocaproate, alpha-ketoisocaproate and isovalerate from leucine. metroni ...	1997	9069531
septic arthritis after arthroscopic anterior cruciate ligament reconstruction. diagnosis and management.	we performed a retrospective study of knee joint infections after arthroscopic anterior cruciate ligament reconstruction at our institution. two thousand five hundred anterior cruciate ligament reconstructions were performed between 1988 and 1993. seven (0.3%) patients experienced postoperative deep infections of the knee. all anterior cruciate ligament reconstructions were performed using arthroscopically assisted techniques. six (86%) of these patients had concomitant open procedures performed ...	1997	9079185
solution structure of the albumin-binding ga module: a versatile bacterial protein domain.	the albumin-binding ga module is found in a family of surface proteins of different bacterial species. it comprises 45 amino acid residues and represents the first known example of contemporary module shuffling. using 1h nmr spectroscopy we have determined the solution structure of the ga module from protein pab, a protein of the anaerobic human commensal and pathogen peptostreptococcus magnus. this structure, the first three-dimensional structure of an albumin-binding protein domain described, ...	1997	9086265
a comparison of susceptibility results of the bacteroides fragilis group and other anaerobes by traditional mic results and statistical methods.	antimicrobial susceptibility is compared by mic values reflecting activity by weight and/or percentage of strains resistant. this study establishes these parameters in vitro in the traditional fashion and extends analysis statistically for various groups of anaerobic bacteria. mode mic, mic50, mic90 values and percentage of strains resistant indicated comparable activity for most of the beta-lactams. geometric and arithmetic mics suggested ceftizoxime to be the most active beta-lactam. statistic ...	1997	9096180
[bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. special references to bacteria isolated between july 1995 and june 1996].	isolated bacteria from infections in general surgery during the period from july 1994 to june 1995 were investigated in a multicenter study in japan, and the following results were obtained. one hundred and sixty-four strains were isolated from primary infections, and 202 strains were isolated from postoperative infections. from primary infections, anaerobic gram-positive bacteria were predominant, while from post operative infections, aerobic gram-positive bacteria were predominant. among aerob ...	1997	9100076
periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants.	the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ...	1997	9100195
[the detection of peptostreptococcus asaccharolyticus and bacillus cereus in clinical materials from a child who died of sepsis with a fulminant clinical course].	a case of an acute disease with a rapid clinical course and a fatal outcome in the presence of irreversible toxicoinfectious shock, appearing in two children after the consumption of sheep kidneys, is described. the post mortem examination of the children revealed the presence of hemorrhagic, erosive and necrotic areas on the mucous membrane of the stomach, the duodenum and the upper section of the small intestine. from the material obtained by probing the stomach of one of the children 6 hours ...	1996	9103069
anaerobic cocci of clinical importance.	the current knowledge is reviewed concerning the anaerobic cocci, in particular those of clinical relevance. the anaerobic cocci are defined and their current taxonomic positions discussed. it is clear that new genera and species await to be characterised fully. the overwhelming majority found in clinical material belong to the genus peptostreptococcus, with the remainder belonging to the veillonellae and, possibly, ruminococci. human infections with other anaerobic cocci are extremely rare. the ...	1996	9069108
electrophoresis of whole-cell soluble proteins of microorganisms isolated from bacteremias in endodontic therapy.	we have previously demonstrated that anaerobic bacteria are the microorganisms most frequently isolated from blood following endodontic therapy of teeth with apical periodontitis. phenotypic characterisation of the isolates suggested that the bacteria in the blood originated from the root canal. the present experiment using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was carried out in an effort to verify these findings, and to further study the microorganisms involved i ...	1996	9021323
development and evaluation of a selective and differential medium for the primary isolation of peptostreptococcus micros.	peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. a selective and differential medium for the primary isolation of p. micros was developed and evaluated. columbia cna agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (p. micros medium: pmm). p. micros has a chara ...	1996	9028263
bacteriological epidemiology and treatment of bacterial vaginosis.	one hundred symptomatic women with clinical bacterial vaginosis (bv) were enrolled in this study. the bacteriological epidemiology of bv and efficacy of oral or vaginal administration of antimicrobial agents for treatment of bv were investigated. the epidemiology of bv was investigated before antimicrobial therapy. fifty patients were treated with oral administration of metronidazole (mtn), 500 mg twice a day, and 50 patients with 2% clindamycin (cldm) phosphate in a vaginal cream, 5 g once a da ...	1996	8751270
polymerase chain reaction-based detection of bacteria in semen.	to determine if the presently used bacterial detection techniques provide accurate and complete profiles of microorganisms found in human semen.	1996	8751749
[antibiotic sensitivity of anaerobic bacteria isolated during one year (1993)].	the aim of this study was to determine the antimicrobian susceptibility of the anaerobe bacteria isolated from clinical samples in the authors' department in 1993.	1996	8756216
[in vitro activity of a new fluoroquinolone, marbofloxacin (ro 09-1168) against strictly anaerobic bacteria and some bacteria from human fecal flora].	as recommended by the eec, the in vitro activity of a veterinary quinolone has to be evaluated on human strains isolated from the gut and especially against anaerobes. thus, the mics for marbofloxacin were determined by the reference agar method (norma nccls m11 a3) using wilkins chalgren medium for the 124 anaerobic strains and mueller hinton agar for e. coli, proteus, enterococcus and lactobacillus. on the whole aerobes, the activity of marbofloxacin was equal or slightly greater to that of of ...	1996	8758470
direct evidence for a two-state protein unfolding transition from hydrogen-deuterium exchange, mass spectrometry, and nmr.	we use mass spectrometry in conjunction with hydrogen-deuterium exchange and nmr to characterize the conformational dynamics of the 62-residue igg binding domain of protein l under conditions in which the native state is marginally stable. mass spectra of protein l after short incubations in d2o reveal the presence of two distinct populations containing different numbers of protected protons. nmr experiments indicate that protons in the hydrophobic core are protected in one population, whereas a ...	1996	8762137
vaginal microflora associated with bacterial vaginosis in japanese and thai pregnant women.	the vaginal flora of 118 japanese and 208 thai pregnant women were investigated for the presence of bacterial vaginosis (bv), bv-associated organisms, and bv-associated enzyme. a similar prevalence of bv was found among the japanese (13.6%) and thai women (15.9%). the microbial flora of women with bv were complex; the mean number of isolates recovered in the bv group was approximately 2 times more than that in a group of healthy women. prevotella species, porphyromonas species, peptostreptococcu ...	1996	8909838
microbiology of infected atopic dermatitis.	bacterial infections occur frequently in lesions of atopic dermatitis (ad). the objectives of the study were to establish the aerobic and anaerobic microbiology of secondarily infected ad.	1996	8915731
microbiology of the transition from acute to chronic maxillary sinusitis.	repeated aspirations of sinus secretions by endoscopy was performed in five patients over a period of 34-50 days and, ultimately, surgical drainage was done in three who presented with acute maxillary sinusitis that did not respond to antimicrobial therapy and became chronic. the aspirates were cultured for aerobic and anaerobic bacteria. most of the bacteria isolated from the first culture were aerobic or facultative bacteria: streptococcus pneumoniae (three isolates), haemophilus influenzae no ...	1996	8918954
pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples.	pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ...	1996	8919784
[orbit complication of sinusitis. a report of 7 cases].	orbital complications of sinusitis were reviewed. seven cases seen in a 6-year period from 1989 to 1994 were classified following chandler. the pathogenesis, classification, diagnosis, and treatment of orbital complications, which are rare and steadily decreasing in frequency, are discussed. because of its possible consequences, this complication should not be overlooked.	1996	8924288
[occurrence of non-spore forming anaerobic bacteria in the upper airways of patients with chronic obstructive pulmonary disease].	the aim of this study was to assess the occurrence of anaerobic non-sporeforming bacteria upper airways flora in the exacerbation of copd. sputum from 35 copd patients was sent to the laboratory in sterile, filled with co2 containers and cultured under anaerobic conditions. in all patients bacteriological tests were positive. the most common anaerobic bacteria were as follows: peptostreptococcus - in 28 pts, fusobacterium - in 27 pts, bacteroides - in 26 pts and prevotella in 24. less common wer ...	1996	8926767
[the study of drug resistance in aerobic and anaerobic bacterial flora to selected antibacterial drugs].	the aim of the study to determine the resistance to certain antibacterial drugs of the aerobic and anaerobic bacterial flora isolated from patients operated on for acute abdominal infections. the resistance was investigated by the disc diffusion method. among the isolated aerobes enterobacteriaceae, enterococcus spp, pseudomonas spp. staphylococcus spp were most frequent, and in the group of anaerobes the most frequent were bacteroides spp, peptostreptococcus spp and peptococcus spp. the sensiti ...	1996	8926770
[antimicrobial activities of clarithromycin against clinical isolates].	to examine the antimicrobial activity of clarithromycin (cam) against strains clinically isolated from outpatients in 1994, minimum inhibitory concentrations (mics) were determined for cam and the control drugs. the results were as follows; 1. mic50 and mic90 of cam were similar to those investigated in 1980's against many bacterial species. 2. cam showed strong antimicrobial activities against beta-lactamase producing moraxella subgenus branhamella catarrhalis, bordetella pertussis, campylobact ...	1996	8935125
degradation of host protease inhibitors and activation of plasminogen by proteolytic enzymes from porphyromonas gingivalis and treponema denticola.	bacterial proteases may participate in the pathogenesis of periodontal diseases through their action on host proteins. in the present study, the ability of selected periodontopathogens, as well as two proteases isolated from porphyromonas gingivalis and treponema denticola, to degrade host protease inhibitors was evaluated. the activation of human plasminogen by the two bacterial proteases was also investigated. proteolytic breakdown of host protease inhibitors (alpha-1-antitrypsin, antichymotry ...	1996	8936322
the vaginal microflora of pig-tailed macaques and the effects of chlorhexidine and benzalkonium on this ecosystem.	to characterize normal vaginal microflora of pig-tailed macaques and to evaluate two commonly used intravaginal compounds, chlorhexidine (chg), a vaginal antiseptic (surgilube, e. fougera, melville, ny), and benzalkonium chloride (bzk) (spermicidal contraceptive) in this monkey model to assess effects on the vaginal microflora.	1996	8946634
in vitro susceptibility of anaerobes to quinolones in the united states.	the in vitro activity of early fluoroquinolone antibodies--including ciprofloxacin, ofloxacin, fleroxacin, pefloxacin, enoxacin, and lomefloxacin--against most anaerobes has been limited, a characteristic making them poor choices as antianaerobic agents. newer fluoroquinolones, including levofloxacin, sparfloxacin, and grepafloxacin, have moderate activity against anaerobes, including the bacteroides fragilis group as well as clostridium, peptostreptococcus, prevotella, and fusobacterium species ...	1996	8953099
comparison of activities of trovafloxacin (cp-99,219) and five other agents against 585 anaerobes with use of three media.	agar dilution testing was used to compare the in vitro activities of trovafloxacin and ofloxacin, ciprofloxacin, imipenem, metronidazole, and clindamycin against 585 anaerobes. the activity of trovafloxacin was superior to those of ofloxacin and ciprofloxacin, with 94%, 91%, 96%, 100%, 90%, and 100% inhibition of bacteroides fragilis, non-fragilis bacteroides species, peptostreptococcus magnus/peptostreptococcus micros, clostridium perfringens, prevotella bivia/prevotella disiens, and fusobacter ...	1996	8953106
bacteriology of dentoalveolar abscesses in patients who have received empirical antibiotic therapy.	we investigated aspirates of pus from dentoalveolar abscesses in 13 patients who had received empirical antibiotic therapy within 1 month before presentation at our facility. the antibiotics administered were lincomycin, amoxicillin, ampicillin, or a cephalosporin. information on antibiotic therapy was obtained from the patients' medical histories. cultures of all dentoalveolar abscesses yielded organisms. a total of 70 isolates (42 anaerobes and 28 facultative organisms) were recovered, represe ...	1996	8953107
emerging resistance of anaerobic bacteria to antimicrobial agents in south korea.	in previous studies, bacteroides fragilis group organisms isolated from korean patients were more frequently resistant to various antimicrobial agents, including clindamycin, than were isolates in other countries. a recent report of increased resistance of peptostreptococcus species prompted us to include such isolates in a study of antimicrobial susceptibility. anaerobes isolated in 1994 at a tertiary care hospital in seoul were tested by agar dilution method. none of the b. fragilis group orga ...	1996	8953110
the adherence of periodontopathogens to periodontal probes. a possible factor in intra-oral transmission?	periodontal probes have previously been shown to harbor several bacterial types or species after probing periodontally diseased pockets. this study aims to identify and quantify periodontopathogens that may adhere to a periodontal probe by culturing techniques. it also examines the probe's roughness on its capability to collect bacteria, comparing merrit-b probes (with deep indentations) with tps probes (with smooth surfaces). from the differential phase contrast microscopy findings it was seen ...	1996	8959565
degradation of lactoferrin by periodontitis-associated bacteria.	the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ...	1996	8961558
[successful scartectomy and cryoablation for ventricular tachycardia occurring late after correction of tetralogy of fallot].	a 26-year-old man had a total repair of tetralogy of fallot at 1 year of age, and had redo surgery for restenosis of the right ventricular outflow tract and small residual vsd at 11 years of age. after the second operation, av block developed and an endocardial pacemaker system was implanted. for the last 3 years, he had mild febrile episodes, cough, occasional hemoptysis and paroxysmal ventricular tachycardia. because of his refractory tachycardia and suspected infective endocarditis, he was ad ...	1996	8964997
[role of 67 gallium citrate and 99mtc-hmpao-labelled neutrophils in the integrated diagnostic imaging in a case of peptostreptococcus anaerobius hepatic abscess].		1996	8966261
an rrna approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination.	ruminal amino acid degradation is a nutritionally wasteful process that produces excess ruminal ammonia. monensin inhibited the growth of monensin-sensitive, obligate amino acid-fermenting bacteria and decreased the ruminal ammonia concentrations of cattle. 16s rrna probes indicated that monensin inhibited the growth of peptostreptococcus anaerobius and clostridium sticklandii in the rumen. clostridium aminophilum was monensin sensitive in vitro, but c. aminophilum persisted in the rumen after m ...	1996	8975611
clinical significance of dental root canal microflora.	previous work by this group has shown that a significant association exists between pain and the presence of either prevotella or peptostreptococcus spp. in dental root canals. the aim of this study was to examine a more extensive series of canals microbiologically, to determine whether any other particular endodontic symptoms or clinical signs showed specific associations with individual bacterial species.	1996	8636492
backbone dynamics of a domain of protein l which binds to immunoglobulin light chains.	protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule interacts with the variable domain of immunoglobulin (ig) light chains through five repeated homologous domains denoted b1 to b5. the fold of the ig-light-chain-binding b1 domain of protein l (plb1) has been shown to comprise an alpha-helix packed against a four-stranded beta-sheet and therefore resembles the structure of the igg-binding domains o ...	1996	8654399
effect of co2 on the fermentation capacities of the acetogen peptostreptococcus productus u-1.	the fermentative capacities of the acetogenic bacterium peptostreptococcus productus u-1 (atcc 35244) were examined. although acetate was formed from all the substrates tested, additional products were produced in response to co2 limitation. under co2-limited conditions, fructose-dependent growth yielded high levels of lactate as a reduced end product; lactate was also produced under co2-enriched conditions when fructose concentrations were elevated. in the absence of supplemental co2, xylose-de ...	1996	8655492
isolation of non-sporing anaerobic rods from infections in children.	from 1974 to 1994, 2033 microbiological specimens from children were submitted for cultures for anaerobic bacteria. fifty-seven isolates of bifidobacterium spp. were obtained from 55 (3%) children, 67 isolates of eubacterium spp. from 65 (3%) children and 41 isolates of lactobacillus spp. from 40 (2%) children. most bifidobacterium isolates were from chronic otitis media, abscesses, peritonitis, aspiration pneumonia and paronychia. most eubacterium isolates were from abscesses, peritonitis, decu ...	1996	8667406
bacteremia shortly after placental separation during cesarean delivery.	to assess frequency, risk factors, and microbiology of bacteremia within 15 minutes of placental separation during cesarean delivery.	1996	8677085
[computed tomographic demonstration of intraspinal air after an infection with peptostreptococci].		1996	8679984
[diagnosis and treatment of local forms of surgical anaerobic non-clostridial infection].	145 patients with such kind of surgical infection have been taken to clarify the cause of it. aerobic agents were the cause in 16.6% and anaerobic nonclostridial agents--in 33.1% of all cases. the mixed flora was the cause in 44.1%. the most important place among anaerobic nonclostridial infections belongs to bacteroids (38.3%), peptostreptococcus (27.3%) and the peptococcus (17.2%). one must use wide-spectrum antibiotic for a treatment of a such kind of infection tiel the usults of microbiologi ...	1996	8683929
fetal fibronectin and microorganisms in vaginal fluid of healthy pregnant women.	to determine fetal fibronectin in vaginal fluid from healthy women during uncomplicated pregnancy and to investigate possible relationships to gestational age and the vaginal microflora.	1996	8693926
[a case of the greater omentum abscess due to peptostreptococcus ssp].	a case of the greater omentum abscess due to peptostreptococcus ssp. is reported. a 63-year-old male was admitted to heisei memorial hospital with a complaint of abdominal pain. an intraperitoneal mass measuring 6 cm in diameter was detected by ct and ultrasonogram. he was diagnosed as having an intraperitoneal abscess. after antibiotics therapy by cefotiam, laparotomy was performed and an abscess about 7 cm in diameter at the edge of the greater omentum was found. there was no evidence of perfo ...	1996	8699102
microbiology of subperiosteal orbital abscess and associated maxillary sinusitis.	aspirate of pus from eight subperiosteal orbital abscesses (spoas) and their corresponding infected sinuses were studied for aerobic and anaerobic bacteria. polymicrobial flora was found in all instances, and the number of isolates varied from two to five. anaerobes were recovered from all specimens. the predominant isolates were peptostreptococcus spp, prevotella spp, fusobacterium spp, staphylococcus aureus, and microaerophilic streptococci. concordance in the microbiological findings between ...	1996	8699891
pyomyositis in children, caused by anaerobic bacteria.	the author describes the microbiology and clinical features of six pyomyositis infections in children, which yielded anaerobic bacteria. anaerobic bacteria alone were recovered in four instances, and they are mixed with facultative bacteria in two. there were 15 bacterial isolates (13 anaerobic, 2 facultative). the bacteria were peptostreptococcus sp (5 isolates), bacteroides fragilis (3), clostridium sp (2), fuso-bacterium nucleatum (1), prevotella sp (1), bateroides sp (1), streptococcus pyoge ...	1996	8708910
identification of interdomain sequences promoting the intronless evolution of a bacterial protein family.	in the evolution of eukaryotic genes, introns are believed to have played a major role in increasing the probability of favorable duplication events, chance recombinations, and exon shuffling resulting in functional hybrid proteins. as a rule, prokaryotic genes lack introns, and the examples of prokaryotic introns described do not seem to have contributed to gene evolution by exon shuffling. still, certain protein families in modern bacteria evolve rapidly by recombination of genes, duplication ...	1996	8710897
anaerobic septic bursitis: case report and review.		1996	8722967
in vitro activity of the tricyclic beta-lactam gv104326.	gv104326 is a novel tricyclic beta-lactam (a trinem or, formerly, tribactam). the in vitro activity of gv104326 was compared with those of cefuroxime, cefixime, amoxicillin, amoxicillin-clavulanic acid, cefpirome, and ciprofloxacin. gv104326 had in vitro activity generally similar to that of cefixime against members of the family enterobacteriaceae (mic at which 90% of the isolates are inhibited [mic90], < or = 2 micrograms/ml), with cefuroxime and amoxicillin-clavulanic acid being 8- to 32-fold ...	1996	8723475
antibacterial activity of quinupristin/dalfopristin. rationale for clinical use.	most gram-positive organisms are highly susceptible to the streptogramin, quinupristin/dalfopristin (rp 59500; synercid). minimum inhibitory concentrations for 90% of isolates (mic90) were < or = 1 mg/l for staphylococcus aureus, s. epidermidis, s. haemolyticus, streptococcus pneumoniae, s. pyogenes and listeria monocytogenes. importantly, quinupristin/dalfopristin shows similar activity against methicillin-susceptible and -resistant strains of s. aureus, and streptococci with benzylpenicillin ( ...	1996	8724814
response to periodontal therapy in patients with high or low levels of p. gingivalis, p. intermedia, p. nigrescens and b. forsythus.	in a previous study, subjects receiving either adjunctive tetracycline or augmentin showed, on average, more attachment level gain 10 months post-therapy than subjects receiving either ibuprofen or a placebo, although some subjects in each treatment group showed loss of attachment post-therapy. since differences in treatment response might have been due to differences in the subgingival microbiota, the response to different therapies in subjects with different pre-therapy subgingival microbiotas ...	1996	8739165
utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care.	the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ...	1996	8739166
comparison of spiral gradient endpoint and agar dilution methods for susceptibility testing of anaerobic bacteria: a multilaboratory collaborative evaluation.	a multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (sge) method for the determination of the antimicrobial susceptibilities of anaerobes and to evaluate the equivalence of the mics obtained by the sge method with those obtained by the reference agar dilution method of the national committee for clinical laboratory standards. the standard deviation of the mic obtained by the sge method for the five participating laboratories was +/- 0.26 of ...	1996	8748295
[in vitro activities of sulopenem, a new parenteral penem, against anaerobes].	in vitro activities of sulopenem, a novel parenteral penem, was compared with those of imipenem, flomoxef, cefuzonam, cefoperazone and sulbactam/ampicillin against 66 reference strains (19 genera, 61 species) and 392 recent clinical isolates of anaerobic bacteria and fastidious aerobic bacteria. sulopenem had a very broad spectrum against anaerobic bacteria. in general, this compound was active against anaerobic reference strains with mics of < or = 0.78 micrograms/ml, while being the least acti ...	1996	8786627
susceptibilities of bovine summer mastitis bacteria to antimicrobial agents.	the susceptibility to 9 antimicrobial agents of 32 aerobic bacterial isolates and to 10 antimicrobial agents of 37 anaerobic bacterial isolates from 23 cases of bovine summer mastitis (16 actinomyces pyogenes isolates, 8 streptococcus dysgalactiae isolates, 3 s. uberis isolates, 3 s. acidominimus isolates, 2 streptococcus spp., 15 peptostreptococcus indolicus isolates, 10 fusobacterium necrophorum isolates, and 12 isolates of anaerobic gram-negative rods) was determined by the agar dilution meth ...	1996	8787898
microbiology of acute purulent pericarditis. a 12-year experience in a military hospital.	to study the aerobic and anaerobic micro-biological and clinical characteristics in 15 cases of acute pericarditis treated over a 12-year period.	1996	8790081
microbiology of periapical abscesses and associated maxillary sinusitis.	aspirate of pus from 5 periapical abscesses of the upper jaw and their corresponding maxillary sinusitis were studied for aerobic and anaerobic bacteria. polymicrobial flora were found in all instances, where the number of isolates varied from 2 to 5. anaerobes were recovered from all specimens. the predominant isolates were prevotella sp., porphyromonas sp., fusobacterium nucleatum, and peptostreptococcus sp. concordance in the microbiological findings between periapical abscess and the maxilla ...	1996	8794972
clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects.	thirteen periodontally healthy subjects were monitored clinically for 6-12 months. clinical measurements at 6-weekly intervals included duplicate pd measurements, presence of plaque, redness, and bleeding on probing. baseline measurements consisted of 2 visits 1 wk apart. microbial samples were taken from 11 of the subjects who had completed at least 8 months of monitoring. levels of serum antibodies to 12 periodontal species were determined from 10 subjects. standard deviations of replicate pd ...	1996	8814590
bacteriologic findings in tonsillitis and pericoronitis.	bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ...	1996	8816129
amplified expression and large-scale purification of protein l'.	the gene fragment (ppl') encoding the functional unit of peptostreptococcus protein l was isolated using pcr and expressed in e. coli. as the gene fragment lacked its own promoter, the 5' pcr primer was designed to incorporate an nde1 restriction site (catatg) into the gene. this enabled the gene to be cloned in frame into an nde1 restriction site immediately downstream of a trp promoter. to prevent read through, a stop codon was introduced into the 3' primer. expression of ppl' was up to 27% to ...	1996	8818265
a purgative action of barbaloin is induced by eubacterium sp. strain bar, a human intestinal anaerobe, capable of transforming barbaloin to aloe-emodin anthrone.	orally administered barbaloin (100 mg/kg) did not induce any diarrhea in male wistar rats, in spite of severe diarrhea with sennoside b (40 mg/kg). also, in gnotobiote rats mono-associated with peptostreptococcus intermedius, a human intestinal anaerobe capable of reducing sennidins to rhein anthrone, barbaloin did not induce diarrhea; the faecal water content (71.9%) 8 h after the administration of barbaloin was not increased, compared with that (73.9%) just before the treatment. however, sever ...	1996	8820926
changing anaerobic spectrum in suppurative lung disease: a case report.	a spectrum of three different anaerobes were isolated from a debilitated patient with suppurative lung disease, within a two-year period. repeated isolation from three consecutive samples and symptomatic relief with metronidazole provide clinical evidence of anaerobic lung infection. this case emphasizes the importance of anaerobic culture in cases of protracted pulmonary suppurative disease.	1996	8822645
[antimicrobial activities of tosufloxacin against recently obtained clinical isolates].	to evaluate antimicrobial activities of tosufloxacin (tflx), minimum inhibitory concentrations (mics) of tflx and other drugs were determined against clinical isolates that were obtained in our laboratory from february of 1993 to january of 1994 (period i), and from february of 1995 to january of 1996 (period ii). the results are summarized as follows: 1. compared to mic90's reported by others for tflx in the 1980's, those obtained by us against beta-streptococci, streptococcus pneumoniae, pepto ...	1996	8828073
the dynamics of microbial colonization of barrier membranes for guided tissue regeneration.	the microbial colonization of expanded polytetrafluoroethylene membrane by putative periodontopathogens at 3 minutes of intraoral manipulation was determined in 42 patients with 42 mandibular posterior two- to three-wall defects. twenty patients exhibited no periodontal pockets of > or = 5 mm, other than the study site, and low levels of pathogens (group a). twenty-two patients revealed multiple periodontal pockets of 5 mm or more and numerous pathogens (group b). within the preceding 3 months o ...	1996	8832481
sodium-dependent succinate decarboxylation by a new anaerobic bacterium belonging to the genus peptostreptococcus.	an anaerobic bacterium was isolated from a polluted sediment, with succinate and yeast extract as carbon and energy sources. the new strain was gram-positive, the cells were coccal shaped, the mol% g+g content of the genomic dna was 29, and the peptidoglycan was of the l-ornithine-d-glutamic acid type. comparative sequence analysis of the 16s rrna gene showed the new strain to belong to the genus peptostreptococcus. succinate, fumarate, pyruvate, 3-hydroxybutyrate and lysine supported growth. su ...	1996	8836437
aerobic and anaerobic microbiology of necrotizing fasciitis in children.	specimens obtained from eight children with necrotizing fasciitis (nf) were cultured for aerobic and anaerobic bacteria. a total of 21 isolates were recovered, 13 anaerobic and 8 aerobic or facultatives. the facultative organism streptococcus pyogenes was present alone in two (25%) instances, and mixed aerobic and anaerobic bacteria were isolated in six (75%). the predominant isolates were peptostreptococcus spp. (6 isolates, including 3 peptostreptococcus magnus). s. pyogenes (4), bacteroides f ...	1996	8844744
infection of a total knee joint prosthesis by peptostreptococcus micros and propionibacterium acnes in an elderly ra patient: implant salvage with longterm antibiotics and needle aspiration/irrigation.	we describe the favourable long-term outcome after late total knee joint prosthesis infection in an elderly ra patient. peptostreptococcus micros and propionibacterium acnes were cultured from the synovial fluid, and the finding of a coexistent abscess at the root of a wisdom tooth suggested a dental origin to the joint infection. long-term antibiotics in conjunction with aspiration/irrigation resulted in salvage of the implant and an excellent functional outcome.	1996	8853177
characterization of the binding properties of protein lg, an immunoglobulin-binding hybrid protein.	protein lg is a 50-kda hybrid molecule containing four ig-light-chain-binding domains from protein l of peptostreptococcus magnus and two igg-fe-binding repeats from streptococcal protein g. here we analyse the binding of protein lg to ig from several mammalian species. protein lg was shown to bind human igg of all subclasses and other ig classes that carry kappa chains. the binding to human igg was only marginally influenced by changes in temperature (4-37 degrees c) or salt concentration (0-1. ...	1996	8856054
acute phase response in heifers with experimentally induced mastitis.	ten pregnant heifers were inoculated in both hind udder quarters with a mixture of actinomyces pyogenes, fusobacterium necrophorum and peptostreptococcus indolicus. development of the experimental mastitis was monitored by sequential clinical and bacteriological examinations, and by blood acute phase protein profiles. sequential changes in plasma fibrinogen, serum haptoglobin, acid-soluble glyco-proteins and alpha 1-proteinase inhibitor activity were analysed and compared with both the clinical ...	1996	8864931
deep neck infections.		1996	8870932
in vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.	the antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the national committee for clinical laboratory standards-approved wadsworth agar dilution technique. the species tested included bacteroides fragilis (n = 91), other members of the b. fragilis group (n = 130), campylobacter gracilis (n = 15), other bacteroides spp. (n = 16), prevotella spp. (n = 49), porphyromonas spp. (n = 15), fusobacterium spp. (n = 62), bilophila wadsworthia (n = 24), sutterel ...	1996	8878617
protein pab, an albumin-binding bacterial surface protein promoting growth and virulence.	the anaerobic bacterium peptostreptococcus magnus is a human commensal and pathogen. previous work has shown that strains of p. magnus isolated from patients with gynecological disease (vaginosis) frequently express an immunoglobulin (ig) light chain-binding protein called protein l. here we report that strains isolated from localized suppurative infections bind human serum albumin (hsa), whereas commensal isolates bind neither ig nor hsa. the hsa-binding protein pab was extracted from the bacte ...	1996	8900134
in vitro activity of quinupristin/dalfopristin (rp 59500) against a large collection of infrequently isolated or tested species.	quinupristin/dalfopristin (rp 59500, synercid) is a parenteral streptogramin combination antimicrobial that possesses a synergistic and often bactericidal action against many grampositive species. in this study, a collection of 1270 uncommonly isolated or tested strains were evaluated for susceptibility to quinupristin/dalfopristin using agar dilution minimum inhibitory concentration (mic) methods described in the national committe for clinical laboratory standards. the greatest antimicrobial ac ...	1996	8902412
molecular analysis of microflora associated with dentoalveolar abscesses.	the microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16s rrna genes were randomly amplified by means of conserved eubacterial primers and cloned. restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16s rrna from the cultured bacteria was used to detect putative unculturable bacteria. clones representative of five predominant groups of uncultured organisms were sequenced. two were identified as porp ...	1996	8904410
aerobic and anaerobic microbiology of superficial suppurative thrombophlebitis.	to study the aerobic and anaerobic microbiologic characteristics of superficial suppurative thrombophlebitis.	1996	8546586