Publications

bile acid synthesis in man. in vivo activity of the 25-hydroxylation pathway.	during biosynthesis of bile acid, carbons 25-26-27 are removed from the cholesterol side-chain. side-chain oxidation begins either with hydroxylation at the 26-position, in which case the three-carbon fragment is released as propionic acid, or with hydroxylation at the 25-position, in which case the three-carbon fragment is released as acetone. we have previously shown in the rat that the contribution of the 25-hydroxylation pathway can be quantitated in vivo by measuring production of [14c]acet ...	1988	3134400
smokeless tobacco control. report of a who study group.		1988	3145646
effects of maternal exposure to polychlorobiphenyls (pcbs) on f1 generation behavior in the rat.	the effect of fenclor 42 (pcb) exposure of female rats (fischer 344 strain) was studied through assessment of the behavioral development of their f1 progeny. female rats were exposed to pcb according to the following treatment schedule: (a) (5 days) 2 weeks prior to mating, (b) during gestation (days 6-15 of pregnancy), (c) during lactation (days 1-21 after delivery). behavioral endpoints of motor reflexes, motor coordination, activity (preweaning behaviors), and learning (postweaning behavior) ...	1988	3146520
evaluation of the quality of bacteriologic methods used to diagnose tuberculosis in argentina.		1988	3147118
clonidine hyperphagia: neuroanatomic substrates and specific function.	recent studies have indicated that the alpha 2-noradrenergic agonist clonidine (clon), when peripherally and centrally administered, potentiates feeding in satiated rats in a manner similar to that observed following injection of norepinephrine (ne) into the hypothalamic paraventricular nucleus (pvn). the present experiments examined the effects of clon on meal patterns and macronutrient selection and compared these findings to earlier ne-stimulated feeding studies. administration of clon (25 nm ...	1988	3147461
structure of the human liver/bone/kidney alkaline phosphatase gene.	in man, there are multiple forms of alkaline phosphatase encoded by at least three homologous genes: placental, intestinal, and liver/bone/kidney. this report describes the characterization of the human liver/bone/kidney alkaline phosphatase locus. the gene appears to exist as a single copy in the haploid genome and is comprised of 12 exons distributed over more than 50 kilobases. in liver, kidney, saos-2 human osteosarcoma cells, and cultured fibroblasts, there is a single major start for trans ...	1988	3165380
[metabolic criteria of viability of isolated cardiomyocytes].		1988	3179764
[metabolic criteria of viability of isolated cardiomyocytes].		1988	3179764
subendocardial and subepicardial wall thickening during ischemia in exercising dogs.	to determine whether ischemia in the exercising dog is associated with preservation of subepicardial thickening relative to subendocardial thickening, 10 dogs were chronically instrumented with circumflex artery flow probes, hydraulic occluders, and pairs of ultrasonic microcrystals for determination of wall thickness in the circumflex artery distribution. one pair of crystals spanned the entire ventricular wall (transmural), and the other spanned the outer half of the ventricular wall. inner wa ...	1988	3180383
determination of u in human tissues by delayed neutron activation analysis.	a rapid, sensitive and highly selective technique using delayed neutron activation analysis (dnaa) has been used to determine u concentrations in human tissues. two different sample preparation techniques were compared: one involves total matrix destruction to a dry ash while the other is a nondestructive preparation of the wet sample. the data obtained from the analyses of the same sample by dnaa of wet tissues, dnaa of ashed tissues and from radiochemical analyses using alpha spectroscopy (a s ...	1988	3198401
protein-related differences in the excretion of nitrosoproline and nitrate by the rat--possible modification of de novo nitrate synthesis.	male ola:sd rats were fed purified diets containing 5 or 20% lactalbumin as the source of protein, and the daily urinary excretion of nitrate and nitrosoproline was measured. animals fed the high-protein diet consistently excreted more nitrate and nitrosoproline than littermates fed the low-protein ration, despite a similar, negligible amount of nitrate in both diets. furthermore, whereas nitrite administration enhanced nitrosoproline excretion in both diet groups, nitrate administration increas ...	1988	3220325
phosphorus-31 nuclear magnetic resonance spectroscopic study of the canine pancreas: applications to acute alcoholic pancreatitis.	the first nuclear magnetic resonance spectroscopic study of the canine pancreas is described. both in-vivo, ex-vivo protocols and nmr observables are discussed. the stability of the ex-vivo preparation based on the nmr observables is established for at least four hours. the spectra obtained from the in-vivo and ex-vivo preparations exhibited similar metabolite ratios, further validating the model. metabolite levels were unchanged by a 50% increase in perfusion rate. only trace amounts of phospho ...	1988	3223430
influence of 1-dodecylhexahydro-2h-azepin-2-one (azone) on the in vitro permeation of verapamil hydrochloride across rat, hairless mouse, and human cadaver skin.	the effect of 1-dodecylhexahydro-2h-azepine-2-one (azone; laurocapran; 1) on the permeation of verapamil hydrochloride (2) through rat skin was studied. compound 1 was used in various concentrations in the donor phase. the permeation of the drug increased significantly in the presence of 1 and the enhancement in permeation was dependent on the concentration of 1. a comparison of the enhancing effect of 3% 1 on permeation of 2 through rat, hairless mouse, and human cadaver skin was made. compound ...	1988	3225773
recovery following acute obstruction of the retinal and choroidal circulations. a case history.	the authors report a 70-year-old man who presented with a combined obstruction of both the central retinal artery and the lateral posterior ciliary artery that spontaneously reperfused after 4 days of documented ischemia. despite this prolonged period of decreased perfusion, visual acuity improved from light perception to 20/30 with the re-establishment of normal retinal and choroidal blood flow as documented with intravenous fluorescein angiography. the theory of retinal tolerance time to obstr ...	1988	3231917
epithelium-dependent relaxation of guinea-pig tracheal smooth muscle by carbachol.	modulation of the effect of carbachol by epithelium on tracheal smooth muscle of guinea-pig was studied by using coaxial bioassay system. the contractile response of the bioassay organ (epithelium-free tracheal smooth muscle of guinea-pig) to carbachol was well maintained along the observation period of 30 min. when the same bioassay organ was taken into epithelium-intact tracheal tube of guinea-pig, a significant relaxation followed the carbachol-induced initial contraction within 30 min which ...	1988	3233050
competitive direct enzyme-linked immunosorbent assay for detection of sulfamethazine residues in swine urine and muscle tissue.	a sensitive assay for the detection of sulfamethazine in swine urine and muscle tissue using a direct competitive enzyme-linked immunosorbent assay (elisa) has been developed. undiluted urine or a phosphate-buffered saline extract of pork muscle tissue is mixed with an enzyme-labeled conjugate of sulfamethazine and horseradish peroxidase. the mixture is added to wells of a microtiter plate coated with antibody to sulfamethazine. after the test system is incubated, washed, and re-incubated with s ...	1988	3240969
postnatal brain growth and allometry in the rabbit oryctolagus cuniculus.	a new four-parameter version of pütter's growth curve (pütter, 1920) recently developed by jolicoeur and pirlot (1988) is applied to cross-sectional data on brain and body weight in 66 male rabbits (oryctolagus cuniculus) ranging up to one year in postnatal age. apart from the olfactory bulb, in which there appears to be no initial delay, the estimated initial delays range from 21.7 days (after fertilization) for the cerebellum to 29.9 days for the cerebrum, which suggests that the weight of mos ...	1988	3255724
trimethoprim-sulfamethoxazole for acute dysuria in women: a single-dose or 10-day course. a double-blind, randomized trial.	to compare single-dose and 10-day treatment regimens of trimethoprim-sulfamethoxazole in women with acute dysuria, urgency, or urinary frequency.	1988	3257670
recurrent hypercalcemia and elevated 1,25-dihydroxyvitamin d levels in hodgkin's disease.	hypercalcemia has been infrequently associated with hodgkin's disease. when seen, most cases have been attributable to skeletal invasion by disease. herein is described a 40-year-old man with a 15-year history of hodgkin's disease. each of four disease recurrences was heralded by hypercalcemia occurring in the absence of bone disease or elevation of parathyroid hormone levels. marked elevations of 1,25-dihydroxyvitamin d levels were observed that paralleled his disease course and response to the ...	1988	3337120
effects of kainic acid on seizure susceptibility in the developing brain.	the short and long-term effects of systemic administration of kainic acid to immature animals were studied in rats. kainic acid was administered systemically to rats of 1-30 days of age. the rats were monitored for both behavioral and eeg changes. to study the effects of kainic acid on seizure susceptibility, status epilepticus was induced in 12-, 18-, and 27-day-old rats by systemic administration of kainic acid. seizure susceptibility was assessed 3 days later using the kindling technique. in ...	1988	3359330
pharmacokinetic and pathological evaluation of gentamicin in cats given a small intravenous dose repeatedly for five days.	gentamicin was administered to six cats at a dosage of 3 mg/kg of body weight intravenously every 8 h for five days. peak and trough serum gentamicin concentrations were measured after each injection. gentamicin elimination rate and serum half-life were calculated. serum urea nitrogen, creatinine, biochemistry profile, electrolyte, glucose, total protein, and albumin concentrations were measured daily. urinalyses were performed before and after the five-day experimental period. the mean +/- sd p ...	1988	3370552
blood vascular organization of the human appendix: a scanning electron microscopic study of corrosion casts.	blood vascular beds of the human appendix were reproduced with a methacrylate casting medium and observed with a scanning electron microscope. the appendix received some small afferent arterial branches of the appendicular artery. these small arterial branches pierced the muscular layer and reached the submucosal plexus. small arterioles arising from this plexus, climbed the mucosa and formed honeycomb-like capillary meshes at the luminal surface level. these capillaries were drained by the coll ...	1988	3376104
alveolar macrophage subpopulations' responsiveness to chemotactic stimuli.	recent data suggest that alveolar macrophages are a heterogeneous group of cells with several subpopulations. this study was undertaken to determine if there is heterogeneity among rat alveolar macrophage subpopulations ability to respond to chemotactic stimuli. alveolar macrophages were harvested by bronchoalveolar lavage and fractionated into density-defined fractions by centrifugation through a continuous isoosmotic gradient of colloidal silica. unfractionated and density-defined alveolar mac ...	1988	3394797
low-fluence carbon dioxide laser irradiation of lentigines.	low-fluence carbon dioxide (co2) laser irradiation of skin has previously been shown to induce damage limited primarily to the epidermis. to evaluate whether this technique was therapeutically effective for pigmented epidermal lesions, ten lentigines caused by methoxsalen and ultraviolet light therapy were treated in one patient using the co2 laser at fluences ranging from 3.0 to 7.7 j/cm2 for 0.1-s exposures with 4.5-mm spot size. based on substantial clearing in seven of ten lesions treated, 1 ...	1988	3401025
role of endogenous norepinephrine in microcirculation after experimental acute spinal cord injury.	the effects of endogenous norepinephrine on changes in spinal microcirculation after acute injury of the spinal cord were examined in rabbits. 6-hydroxydopamine (6-ohda), which selectively destroys catecholamine neurons, was injected into the subarachnoid cavity of the rabbit spinal cord. measurement of the intraspinal monoamine level revealed a decrease in the intraspinal norepinephrine level in the treated animals. recovery from motor paralysis after compression at t-8 occurred more rapidly in ...	1988	3404014
ultrastructure of the sebaceous glands of the camel (camelus dromedarius).	the sebaceous gland of the camel has been studied by light and electron microscopy. the gland is lobed but the lobes are not subdivided into lobules. the ultrastructure of the sebaceous gland of the camel is generally similar to that of other animals. it consists of cells at different stages of development and maturation. the cells which are programmed to produce lipid secretion are probably those which lose contact with the basal lamina. various forms of smooth endoplasmic reticulum are seen in ...	1988	3417542
improved method for determination of plasma pindolol by high-pressure liquid chromatography with ultraviolet detection.		1987	3427212
analysis of epidermal proteins for dna-binding activity.	a group of dna-binding proteins from the soluble extract of newborn rat epidermis have been separated by chromatography using dna-cellulose columns. the electrophoretogram of the dna-binding proteins eluted from a single stranded dna-cellulose column shows five major proteins of molecular weights ranging between 25k to 40k. both the epidermal protein filaggrin and most keratins, except two high molecular weight keratins, do not show in vitro dna-binding activity.	1987	3427216
[seropositivity in occupationally exposed persons to toxoplasmosis, q-fever and chlamydiosis].		1987	3427492
actions of prostaglandins e2 and f2 alpha on release of 14c-labelled mucins from rat submandibular salivary acini in vitro.	mucin secretion was studied in vitro using well-characterized preparations of isolated acini. pge2 significantly (p less than 0.05) increased release of [14c]-glucosamine labelled mucins at the highest concentration tested (10(-5) m), but was ineffective at lower doses (10(-9)-10(-6) m). pgf2 alpha had no effect on mucin secretion over this concentration range. pge2 (10(-9)-10(-5) m) did not modify isoproterenol stimulated mucin secretion. the cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) ...	1987	3482153
characterization of feline t and b cells.	feline peripheral-blood lymphocyte populations (n = 22) were examined for the following markers: rosette formation with guinea pig erythrocytes (gpe-t cells), rosette formation with human rbc (hrbc-t cells), rosette formation with sheep rbc, mixed rosette formation with gpe-t cells and hrbc-t cells (total t cells), erythrocyte antibody-complement rosettes, and surface immunoglobulin. an average of 28% +/- 7% (range, 16% to 39%) of the feline lymphocytes formed rosettes with gpe-t cells, and 27% ...	1987	3493718
failure of klebsiella pneumoniae antibodies to cross-react with peripheral blood mononuclear cells from patients with ankylosing spondylitis.	cross-reactivity between antibodies to 2 strains of klebsiella pneumoniae (k43 and f77) and the peripheral blood lymphocytes of patients with ankylosing spondylitis (as) was examined in 3 separate antibody binding and cytotoxicity assays. using k pneumoniae antisera in a chromium release cytotoxicity assay, we found no difference in the reactions of cells from as patients and those from control subjects. this result contrasts with the results of previous studies. similarly, using an enzyme-linke ...	1987	3551965
protected methionine supplementation with extruded blend of soybeans and soybean meal for dairy cows.	methionine may be the first amino acid limiting milk production in early lactation cows. to evaluate this further, 23 high producing holstein cows (9 multiparous and 14 primiparous) were fed an extruded blend of soybeans and soybean meal (40:60) without or with 15 g of added dl-methionine as 50 g of ruminally protected methionine product during wk 4 to 16 postpartum. cows were fed a 15.8% crude protein total mixed ration consisting of 30% (dry basis) corn silage, 15% alfalfa hay, and 55% concent ...	1987	3571638
[cardiac defibrillation. experimental and theoretical aspects].		1987	3577372
the polypeptide fold of the globular domain of histone h5 in solution. a study using nuclear magnetic resonance, distance geometry and restrained molecular dynamics.	the polypeptide fold of the 79-residue globular domain of chicken histone h5 (gh5) in solution has been determined by the combined use of distance geometry and restrained molecular dynamics calculations. the structure determination is based on 307 approximate interproton distance restraints derived from n.m.r. measurements. the structure is composed of a core made up of residues 3-18, 23-34, 37-60 and 71-79, and two loops comprising residues 19-22 and 61-70. the structure of the core is well def ...	1987	3608994
abdominal operations: effect on subsequent experimental faecal peritonitis.	rats were subjected to laparotomy, anaesthesia only (controls), laparotomy plus 1-cm-segment ileum isolation, or no treatment (controls) and were challenged with endogenous faecal peritonitis 1 and 2 weeks later, respectively. survival rates were 40% for the laparotomy group and 100% for the ileum isolation group, whereas all controls died. the number of peritoneal leucocytes, particularly macrophages and neutrophils, was significantly (p less than 0.05) higher in the laparotomy group than in th ...	1987	3629184
[a method of quantitative determination of delta 5,7-sterols in unsaponifiable lipid fractions].	the technique is offer for rapid quantitative definition of sterols having a system of delta 5,7-conjugated double bonds which conditions biological activity of d vitamin--the result of photoisomerisation of the sterols. the technique bases on the analysis of uv absorption spectra of unsaponifiable lipid fraction of several kinds of mutant yeast strains and the model mixtures of sterols. the results obtained concerning the estimation of delta 5,7-sterols containing in total fraction are confirme ...	1987	3658928
the duck hepatitis b virus pre-c region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation.	analysis of the serum of duck hepatitis b virus (dhbv)-infected ducks has revealed the presence of c-terminally truncated viral core proteins (e antigens). these proteins are glycosylated and therefore were not released from infected cells by lysis but rather by active secretion, indicating that the dhbv core protein can be synthesized alternatively as a cytoplasmic or a secretory protein. transient expression of cloned wild-type dhbv dna and of a specifically designed viral mutant in a human he ...	1987	3682059
the duck hepatitis b virus pre-c region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation.	analysis of the serum of duck hepatitis b virus (dhbv)-infected ducks has revealed the presence of c-terminally truncated viral core proteins (e antigens). these proteins are glycosylated and therefore were not released from infected cells by lysis but rather by active secretion, indicating that the dhbv core protein can be synthesized alternatively as a cytoplasmic or a secretory protein. transient expression of cloned wild-type dhbv dna and of a specifically designed viral mutant in a human he ...	1987	3682059
some properties of semicarbazide-sensitive amine oxidases.	the semicarbazide-sensitive amine oxidases (ssaos) comprise a substantial but diffuse group of enzymes separable from classical monoamine oxidase in several respects. differences in cofactor requirement, molecular weight and subcellular distribution are crucial for such a separation. differential sensitivity to enzyme inhibitors, characterized by resistance to inhibition by acetylenic mao inhibitors coupled with sensitivity to semicarbazide and some related compounds are characteristic of these ...	1987	3295116
[qualitative and quantitative studies on salmonellae in industrially obtained poultry meat (broilers)].		1987	3297601
a controlled trial of nebulized aminoglycoside and oral flucloxacillin versus placebo in the outpatient management of children with cystic fibrosis.	six children with cystic fibrosis who had persistently had pseudomonas aeruginosa isolated from their respiratory tract, completed a double-blind cross-over comparison of oral flucloxacillin and nebulized aminoglycoside versus double placebo. the patients had higher fev1 results at the end of the month of active treatment than after the month of placebo.	1987	3329531
beta 2 microglobulin enhances the infectivity of cytomegalovirus and when bound to the virus enables class i hla molecules to be used as a virus receptor.	we have previously demonstrated that human cytomegalovirus (cmv) binds the host protein beta 2 microglobulin (beta 2m) from body fluids or from cell culture media. in this report we have examined the effect of the beta 2m on viral infectivity. we have shown that the addition of human purified beta 2m, or a fraction of foetal calf serum corresponding to bovine beta 2m, to culture medium increased the amount of infectious extracellular cmv, compared to that from cells grown in serum-free medium. m ...	1987	3029306
regional variation in high-density lipoprotein binding to human adipocyte plasma membranes of massively obese subjects.	obesity is associated with significant changes in cholesterol and lipoprotein metabolism. high density lipoprotein (hdl) cholesterol is often reduced and adipose tissue cholesterol stores are increased in obese individuals. this prompted a study on the binding of hld fractions (hdl2 and hdl3) to adipocyte plasma membranes obtained from massively obese subjects (bmi greater than 37 kg m-2) undergoing gastroplasty. regional variation in hdl binding to these adipocyte plasma membranes was demonstra ...	1987	3032643
a high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes.	a counterflow centrifugal elutriation procedure for preparing human granulocytes is described. this method permits isolation of 1-2 x 10(9) granulocytes with a yield of 70% +/- 7% from a unit of whole blood in 3 h. these cells are 99% +/- 1% pure and 95-99% viable. when compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. other responses including receptor-me ...	1987	3036986
t and b peripheral blood lymphocytes in normal and lymphocytotic sheep.	surface immunoglobulins (sig), peanut agglutinin (pna), spontaneous erythrocyte rosette (e-rosette) and helix pomatia (hp) marker were investigated in normal and bovine leukemia virus (blv)-infected sheep. in normal sheep, 19.3% +/- 4.9 of peripheral blood lymphocytes (pbl) were sig+, whereas 58% +/- 5.69 were pna+, and 19.6 +/- 5.2 were e-rosette forming cells (e-rfc). in blv-induced lymphocytotic sheep, sig+ cells in pbl reached 59.4% +/- 15.06. in the same animals, pna bound to 20.6% +/- 9.69 ...	1987	3040327
noradrenergic projections to brainstem nuclei: evidence for differential projections from noradrenergic subgroups.	retrograde transport of the fluorescent tracer true blue was used in combination with immunohistochemical staining of dopamine-beta-hydroxylase (a marker protein for noradrenergic neurons) to determine the origin of noradrenergic projections to three cranial nerve nuclei: 1) the motor nucleus of the trigeminal nerve, 2) the motor nucleus of the facial nerve, and 3) the spinal trigeminal nucleus pars interpolaris. noradrenergic cells in the rat brainstem were divided into subgroups and their numb ...	1987	2822772
bevantolol hydrochloride--preclinical pharmacologic profile.	bevantolol hydrochloride, a beta adrenoceptor antagonist, can be categorized using conventional schemes as being cardioselective, devoid of intrinsic sympathomimetic activity and having weak membrane-stabilizing and local anesthetic properties. the cardioselectivity of bevantolol was conferred by the incorporation of a 3,4-dimethoxyphenyl moiety into the terminal amino portion of the molecule. this portion of the molecule also appears to account for bevantolol's in vitro binding affinity at alph ...	1986	2871786
adrenergic and cholinergic stimulation of arachidonate and phosphatidate metabolism in cultured astroglial cells.	primary cultures of neonatal rat brain polygonal astroglia, or cells of the c62b glioma line, were incubated with [1-14c]arachidonic acid (aa) in culture for 18 hr. in both culture systems, more than 80% of the added [1-14c]aa was taken up into cellular glycerolipids; less than 1% of the radioactivity in the cells was present in an unesterified form. when prelabeled c62b cells were stimulated with acetylcholine (ach), there was a rapid accumulation of arachidonyl-phosphatidic acid (pa) accompani ...	1986	2875401
demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report.	isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ...	1986	3002299
effect of dibutyryl cyclic amp on morphologic features and biologic markers of a human salivary gland adenocarcinoma cell line in culture.	dibutyryl cyclic amp (db-camp) has marked effects on the growth, morphologic features, and biologic markers of a human salivary gland adenocarcinoma cell line in culture. a cell line with ultrastructure and biologic markers specific to the intercalated duct cells of human salivary glands was cultivated in the presence of db-camp. major alterations, such as process formation and expression of myofilaments and oxytocin receptor in addition to myosin and s-100 protein, were observed in those cells ...	1986	3002591
central nervous system lesions in suckling mice and rats inoculated intranasally with sialodacryoadenitis virus.	suckling cd-1 and cfw mice and wi wistar rats were inoculated intranasally with sialodacryoadenitis virus. in animals inoculated during the first week of life, there was an acute necrotizing encephalitis with malacia and minimal inflammatory cell response. in mice and rats which survived for up to 12 days post inoculation, loss of brain substance, gitter cells and occasionally mineralized debris were seen. viral antigen was readily demonstrated in neurons and nasal epithelium in trypsin-treated, ...	1986	3004003
significance of intracellular calcium and cyclic adenosine 3',5'-monophosphate in the mechanisms by which prostaglandins influence human sperm function.	the selective ability of pge-1 and pge-2 to enhance the capacity of human spermatozoa for hamster oocyte penetration was dependent upon high concentrations (1.7 mm) of extracellular ca2+ and a prolonged (4 h) duration of exposure, and insensitive to the ca2+ channel antagonist, verapamil. studies with the intracellular calcium indicator quin-2 indicated that exposure to pge-1 and pge-2, but not pgf-2 alpha, induced a significant rise in the levels of cytoplasmic ca2+, suggesting that an ionophor ...	1986	3016256
epstein-barr virus infections in families: the role of children with infectious mononucleosis.	we performed a prospective evaluation of epstein-barr virus (ebv) infections in 78 families with a childhood index case of ebv-infectious mononucleosis (ebv-im). during the acute index episode, adult family (household) contacts, compared with control adults, had a greater rate of oropharyngeal ebv excretion and high serum antibody responses, which suggested a recent antecedent reactivation of an old ebv infection. the increased prevalence of oropharyngeal ebv and acute serological responses foun ...	1986	3021867
[septicemic campylobacteriosis after dog bite].		1986	2945190
effect of mononuclear cell factors on calcium transport in sarcoplasmic reticulum vesicles.	the effect of supernatants from cultures of mitogen-stimulated human mononuclear cells on calcium transport by sarcoplasmic reticulum was examined. calcium transport was assayed by measuring the time course of calcium accumulation by sarcoplasmic reticulum incubated with supernatants from stimulated mononuclear cells was 20% less than that by vesicles exposed to control supernatants (p less than 0.001). in contrast, no difference in calcium-dependent atpase activity was noted between vesicles in ...	1986	2946307
sporidesmin and gliotoxin induce cell detachment and perturb microfilament structure in cultured liver cells.	changes in cell morphology and cell adhesion occurred when cultured cells from the rat liver cell strain c3 were exposed to the fungal toxins, sporidesmin or gliotoxin. both toxins caused loss of attachment of the cells to the plastic of tissue culture plates and this effect was preceded by loss of actin cables. other changes included cytoplasmic vacuolation and blocked entry into s-phase of the cell cycle. under these conditions [3h]thymidine incorporation into the cells was also diminished but ...	1986	2432076
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
metabolism-related tissue-binding of halogenated hydrocarbons.		1986	3101260
structural and functional relationships of grafted vasopressin neurons.	fetal vasopressin neurons were grafted into adult long-evans rats with vasopressin deficiencies created by neural lobe ablation one week prior to implantation. control animals and recipients with ectopic grafts still possessed significant deficits in fluid regulation 6 weeks following implantation. however, recipients with grafts fused to the host median eminence and containing magnocellular vasopressin neurons juxtaposed to the host portal vasculature showed restored peripheral vasopressin acti ...	1986	3708331
reciprocal functional interaction of adjacent myocardial segments during regional ischemia: an intraventricular loading phenomenon affecting apparent regional contractile function in the intact heart.	the mechanism of the increase in function in myocardial segments adjacent to those subjected to regional ischemia is not known. to investigate the hypothesis that changes in regional loading are an important causative factor of this phenomenon, 17 dogs were instrumented with segmental shortening sonomicrometers in the left anterior descending and circumflex artery distributions, circumflex coronary flow probes and cuff occluders, as well as circumflex coronary pressure catheters. additionally, t ...	1986	3711491
reciprocal functional interaction of adjacent myocardial segments during regional ischemia: an intraventricular loading phenomenon affecting apparent regional contractile function in the intact heart.	the mechanism of the increase in function in myocardial segments adjacent to those subjected to regional ischemia is not known. to investigate the hypothesis that changes in regional loading are an important causative factor of this phenomenon, 17 dogs were instrumented with segmental shortening sonomicrometers in the left anterior descending and circumflex artery distributions, circumflex coronary flow probes and cuff occluders, as well as circumflex coronary pressure catheters. additionally, t ...	1986	3711491
reevaluation of the role of gangliosides as receptors for tetanus toxin.	binding of tetanus toxin to rat brain membranes was of lower affinity and capacity when binding was determined in 150 mm nacl, 50 mm tris-hcl (ph 7.4) than in 25 mm tris-acetate (ph 6.0). binding under both conditions was reduced by treating the membranes with neuraminidase. pronase treatment, however, reduced toxin binding only in the tris-saline buffer (ph 7.4). in addition, the concentration of gangliosides required to inhibit toxin binding was 100-fold higher in tris-saline compared to tris- ...	1986	3711900
3,3',5'-triiodothyronine inhibits iodothyronine-5'-deiodinating activity induced by 3,5,3'-triiodothyronine at equimolar concentrations in cultured fetal mouse liver.	to investigate the rt3 effect on iodothyronine-5'-deiodinating activity (i-5'-da) in cultured fetal mouse liver, liver on the 19th day of gestation, in which little or no i-5'-da was detected and, therefore, rt3 was very stable, was cultured in medium containing thyroid hormone-depleted fetal calf serum supplemented with cortisol, insulin, and various concentrations of iodothyronines. after 4-15 days of culture, i-5'-da in the homogenate was assessed by the amount of iodide released from outer r ...	1986	3732155
effect of tick-borne fever on the disposition of sulphadimidine and its metabolites in plasma of goats.	the effect of tick-borne fever (tbf) on the plasma disposition of sulphadimidine (sdm) and its metabolites in goats was studied. in uninfected goats, sdm was extensively metabolised mainly by hydroxylation, glucuronidation and to a minor extent by acetylation. in tbf infected goats the hydroxylation of sdm into 6-methylhydroxysulphadimidine (sch2oh) as well as into 5-hydroxysulphadimidine (soh) was markedly reduced (-57.6 and -63.6 per cent, respectively). an unidentified metabolite (metabolite ...	1986	3738235
[surgical approach to abdominal aortic aneurysm with malignant alimentary tract tumor: report of three cases].	the clinical courses of three cases with various alimentary tract malignant lesions coincidental with abdominal aortic aneurysm were reported. of those three patients, a simultaneous resection of the malignant lesion and aneurysm was carried out in two patients, while an secondary abdominal aneurysmectomy following the resection of the malignant lesion was done in one patient. a 70-year-old man with cancer of the cecum and an infra-renal abdominal aneurysm, was diagnosed preoperatively, and a si ...	1986	3747994
[concentration of anti-brain antibodies in the blood of patients with diffuse toxic goiter and thyrotoxic encephalopathy].	the content of anticerebral antibodies was studied in the peripheral blood of 10 healthy persons, 42 patients with diffuse toxic goiter and 25 patients with thyrotoxic encephalopathy. it was determined by a method of passive hemagglutination using a human brain protein extract as an antigen. in the healthy persons antibodies to cerebral antigens were undetectable whereas in 24 of 42 patients with diffuse toxic goiter without signs of the nervous system involvement they were detected (log2 titer- ...	1986	3754967
in vivo stability of in vitro labelled 99tcm-red blood cells for radionuclide determination of left ventricular ejection fractions and volumes.	serial determination of radionuclide left ventricular ejection fraction and volumes require a label that remains highly stable after introduction into the human circulation. we have evaluated the in vivo stability of 99tcm-red blood cells (99tcm-rbc) labelled in vitro by preparation with small amounts of a stannous agent in 19 patients with coronary artery disease. the distribution volume of 99tcm-rbc was virtually identical to that of rbc labelled with 51cr. labelling efficiency expressed as th ...	1986	3774253
interactive responses to diethylstilboestrol in c3h mice.	female c3heb/fej mice were fed diethylstilboestrol (des) at a dietary concentration of 100 ppb beginning at 6 wk of age. several oestrogen-sensitive target organs (uterus, vagina, bone and mammary gland) were removed and examined histologically when mice died, became moribund, were killed at scheduled intervals or were removed because of presumptive mammary tumours (palpable mass having a 1-cm diameter). oestrogen-sensitive histological observations for which there was less than a 100% prevalenc ...	1986	3781439
[changes and induction of drug-metabolizing enzymes in rats with hepatic injuries].		1986	3804171
[vacuous chewing after haloperidol and gaba-linoleamide administration in the rat].		1986	3812899
[vacuous chewing after haloperidol and gaba-linoleamide administration in the rat].		1986	3812899
[changes in the estrous cycle of the rat after irradiation with a helium-neon laser].		1986	3812911
the place and value of bestophilia in perversions.	an attempt is made to place bestophilia in cultural, clinical, and theoretical perspective. archeological and anthropological documentation and records of the widespread appearance in mythology of this practice are cited. this archaic form of perversion is still practiced in rural environments and isolated communities. the treatment of a man who grew up in such an environment gave the author the opportunity to study this form of perversion. the correlation of clinical findings with culturally sa ...	1986	3819310
ornithine transcarbamylase from mycobacterium smegmatis atcc 14468: purification, properties, and reaction mechanism.	ornithine transcarbamylase (ec 2.1.3.3) has been purified 980-fold from mycobacterium smegmatis and has a molecular weight of 116,000. initial velocity determinations indicated that the reaction proceeds via a sequential kinetic mechanism. the limiting michaelis constants for carbamyl phosphate (kma) and ornithine (kmb) and the dissociation constant for carbamyl phosphate (kia) were found to be 0.20, 0.25, and 0.07 mm, respectively. ornithine at higher concentrations acted as an uncompetitive in ...	1986	3566963
initiation of swimming activity by trigger neurons in the leech subesophageal ganglion. ii. role of segmental swim-initiating interneurons.	cell tr1, a trigger neuron found in the subesophageal ganglion of the leech, hirudo medicinalis, is part of a network of subesophageal ganglion neurons which control swimming activity, and makes apparently direct connections to swim-initiating interneurons (siis; cells 204 and 205). in this study, we investigated the role of siis in swim initiation by cell tr1. we also examined how brief tr1 activity controls swim initiation at the levels of the siis and of the oscillator neurons. we found: in s ...	1986	3023603
immunosuppressive properties of human placenta: study of supernatants from short-term syncytiotrophoblast cultures.	using collagenase and mechanical treatment to attempt to eliminate cellular contamination such as macrophages and decidual cells, trophoblast enriched cell suspensions were isolated from the human placenta. with a view to assessing the role of trophoblast in impairing maternal rejection of the fetus, supernatants (spl4) were prepared from these placental cells after short-term culture (4 h). the immunosuppressive activity of these supernatants was studied following application to mitogen-stimula ...	1986	3023607
synthesis and antiviral evaluation of 1,4-dioxane nucleoside analogues related to nucleoside dialdehydes.	since biologically active nucleoside 2',3'-dialdehydes exist as six-membered cyclic acetals (1) in solution, we have investigated the antiviral activity of some structurally similar 1,4-dioxane nucleoside analogues. by reacting 2',3'-seconucleoside tosylates with base, the guanine (10) and adenine (18) substituted (hydroxymethyl)dioxanes have been constructed. in addition, an unusual adenine-substituted divinyl ether (22) was synthesized via a base-catalyzed, double elimination of a 2',3'-di-o-t ...	1986	3023613
prevalence of leptospira interrogans serovar hardjo in the genital and urinary tracts of non-pregnant cattle.	in a bacteriological investigation of 60 cows and heifers from an abattoir leptospira interrogans serovar hardjo was isolated from 39 (65 per cent) animals. the genital tract (57 per cent) appeared to be as important a site of hardjo localisation as the urinary tract (62 per cent). this is believed to be the first report of genital hardjo infection in naturally infected cattle.	1986	3511601
prevalence of leptospira interrogans serovar hardjo in the genital and urinary tracts of non-pregnant cattle.	in a bacteriological investigation of 60 cows and heifers from an abattoir leptospira interrogans serovar hardjo was isolated from 39 (65 per cent) animals. the genital tract (57 per cent) appeared to be as important a site of hardjo localisation as the urinary tract (62 per cent). this is believed to be the first report of genital hardjo infection in naturally infected cattle.	1986	3511601
effect of chemical sympathectomy on renal hydroelectrolytic handling in dogs with chronic caval constriction.	the effect of renal selective chemical sympathectomy by intrarenal infusion of 6-hydroxydopamine (6-ohda, 5 mg/kg body weight) on the renal excretion of water and electrolytes was studied in 7 dogs in whom a syndrome of sodium and water retention and ascites formation was induced by partial constriction of thoracic inferior vena cava. propranolol (1 mg) and phentolamine (3 mg) were also injected to obviate acute systemic hemodynamic changes. sympathectomy was performed once in 4 dogs and three t ...	1986	3530590
[functional status of the rat insular apparatus and adrenal cortex at early periods after irradiation].	as early as 0.5-3 h after x-irradiation of rats with a lethal dose of 12 gy glucocorticoid activity of adrenal cortex was enhanced and 11-oxycorticosteroid level in blood was increased. the increasing of the immunoreactive insulin content of blood was registered at the background of hypercorticoidism and normal function of insular apparatus during the first hours following irradiation. at later times (24-72 h), a stable hypercorticoidism developed, the ability of beta-cells to react adequately t ...	1986	3534939
[functional status of the rat insular apparatus and adrenal cortex at early periods after irradiation].	as early as 0.5-3 h after x-irradiation of rats with a lethal dose of 12 gy glucocorticoid activity of adrenal cortex was enhanced and 11-oxycorticosteroid level in blood was increased. the increasing of the immunoreactive insulin content of blood was registered at the background of hypercorticoidism and normal function of insular apparatus during the first hours following irradiation. at later times (24-72 h), a stable hypercorticoidism developed, the ability of beta-cells to react adequately t ...	1986	3534939
endotoxin-induced bovine mastitis: immunoglobulins, phagocytosis, and effect of flunixin meglumine.	milk whey immunoglobulins (ig) and phagocytosis of staphylococci by milk polymorphonuclear neutrophilic leukocytes (pmn) were measured in 12 cows (allotted to 6 pairs) during acute bovine mastitis induced by intramammary inoculation of endotoxin. six of these cows (or 1 in each pair) were treated with flunixin meglumine and were compared with the others (given only saline solution). the endotoxin inoculation comprised 10 micrograms of escherichia coli o26:b6 lipopolysaccharide injected into one ...	1986	3538954
histochemical and biochemical urease localization in the periplasm and outer membrane of two proteus mirabilis strains.	proteus mirabilis, a gram-negative bacillus, is often implicated in the formation of infectious kidney stones. as ureolytic activity of this organism is thought to play a major role in its pathogenesis, we adapted our recently described urease localization technique to visualize urease activity in vivo. urease activity was ultrastructurally localized in two clinically isolated p. mirabilis strains by precipitating the enzymatic reaction product (ammonia) with sodium tetraphenylboron. subsequent ...	1986	3539291
hepatitis b virus and primary liver cancer on chongming island, people's republic of china.	data on potential risk factors for primary liver cancer (plc), including hepatitis b virus (hbv) infection, contaminated drinking water, maize consumption, and use of tobacco and alcohol were collected from 12,222 males over 40 years of age living on chongming island, a high-risk area for plc. during the first 3 years of follow-up (1980-82), 70 deaths from this disease occurred among study subjects. preliminary results indicate that hbv carriers were 6.7 times more likely to die of plc than were ...	1985	3834335
shigellosis in children--prevalence of subgroups and antibiotic resistance.		1985	3836974
regulation of the production of granulocyte-macrophage colony-stimulating factor by macrophage-like tumour cell lines.	macrophage tumour cell lines (pu5-1.8, p388d1) produced detectable granulocyte macrophage colony-stimulating (csf-2) activity measured using a factor-dependent cell line fdc-p1. the production of csf-2 was enhanced by endotoxin and inhibited by serum, and correlated inversely with [3h]tdr incorporation. mrna isolated from pu5-1.8 or p388d1 cells initiated csf-2 production when injected into xenopus laevis oocytes. the specific activity in this assay was unaltered in mrna isolated from endotoxin- ...	1985	3871405
expression of a melanoma-tumor-associated antigen as demonstrated by a monoclonal antibody (d6.1) in cytopathologic preparations of human tumor cells from effusions and needle aspirates.	immunocytopathologic studies were performed on 79 fine needle aspiration biopsies (fnabs) and effusions from 13 melanomas and 57 other human neoplasms with the monoclonal antibody (mab) d6.1 raised against a partially purified melanoma-tumor-associated antigen (mtaa). the purposes of these studies were (1) to evaluate the ability of mab 6.1 to react with melanoma cells in cytopathologic preparations and (2) to define the spectrum of reactivity of mab d6.1 in cytopathologic preparations of non-me ...	1985	3890654
the sfia11 mutation prevents filamentation in a response to cell wall damage only in a reca+ genetic background.	n-alpha-palmitoyl-l-lysyl-l-lysine dihydrochloride ethyl ester (pll) at sublethal doses causes filamentous growth of e. coli strains except sfia mutants, which divide normally in its presence. pll does not elicit the sos responses as judged by lambda prophage induction, an increase of reca protein synthesis or induction of the sfia operon in a sfia::lacz fusion strain. thus, it appears that filamentation caused by pll is not an sos function and might be the result of membrane damage by pll, whic ...	1985	3911030
immunocytochemical localization of carbonic anhydrase in the spinal cords of normal and mutant (shiverer) adult mice with comparisons among fixation methods.	the peroxidase-antiperoxidase technique was used for immunocytochemical localization of carbonic anhydrase in the mouse spinal cord to detect whether this antigen was normally present in myelinated fibers, in oligodendrocytes in both white and gray matter, and in astrocytes, and to determine where the carbonic anhydrase might be localized in the spinal cords of dysmyelinating mutant (shiverer) mice. the most favorable methods for treating tissue were: 1) immersion in formalin-ethanol-acetic acid ...	1985	3917467
canine brucellosis in a household.	three cases of canine brucellosis from the same household were discovered during a serosurvey of privately owned dogs. one dog was found to be seropositive and culture-positive, 1 dog was seropositive, and 1 dog had suspect test results, with eye lesions suggestive of brucella canis infection. all dogs had signs seen with b canis infection, but none was pathognomonic. although pathologic findings in 2 of the dogs were consistent with findings reported by other investigators, none was conclusive ...	1985	3921503
[effect of pyruvate dehydrogenase coenzymes and mitochondrial proteins on the accumulation of [35s]lipoic acid].	coenzymes introduced in the ratio, peculiar for pyruvate dehydrogenase complex into the medium containing fresh-isolated mitochondria and oxidation substrate--pyruvate increase accumulation of [35s] lipoate by these organelles. this process is highly stimulated by introducing either the only coa or a coenzyme mixture (coa, thiamine pyrophosphate, fad, nad). addition of phosphate-extracted components of mitochondria and their protein fraction with coenzymes in the ratio indicated above provides m ...	1985	3927544
[effect of pyruvate dehydrogenase coenzymes and mitochondrial proteins on the accumulation of [35s]lipoic acid].	coenzymes introduced in the ratio, peculiar for pyruvate dehydrogenase complex into the medium containing fresh-isolated mitochondria and oxidation substrate--pyruvate increase accumulation of [35s] lipoate by these organelles. this process is highly stimulated by introducing either the only coa or a coenzyme mixture (coa, thiamine pyrophosphate, fad, nad). addition of phosphate-extracted components of mitochondria and their protein fraction with coenzymes in the ratio indicated above provides m ...	1985	3927544
[effect of the homogeneity of the culture, age and amount of the inoculation matter on glucoamylase biosynthesis by aspergillus niger strain b 77].		1985	3929570
accuracy of expiratory carbon dioxide measurements using the coaxial and circle breathing circuits in small subjects.	mass spectrometry is widely used to measure the end-tidal concentrations of inhalation anesthetics and other gases during surgery in order to estimate their arterial concentrations. when certain breathing circuits are used in newborns, however, fresh gas or ambient air may contaminate the expired sample, introducing a systematic error in the measurement of any end-tidal gas concentration. we estimated this error in newborn piglets using carbon dioxide as an indicator substance of expired gas. th ...	1985	3938479
hormone concentrations, mammary development and milk yield in goats given long-term bromocriptine treatment in pregnancy.	ten british saanen goats were treated daily with 5 mg bromocriptine intramuscularly from week 8 of pregnancy until week 20 (day 140). by comparison with untreated control goats (n = 8), concentrations of prolactin in plasma were suppressed throughout the treatment period and remained significantly lower until 3 days prepartum, parturition occurring on day 153 +/- 0.7 (mean +/- s.e.m., n = 10). growth hormone concentrations were low, but the incidence of levels exceeding 1 microgram/l was increas ...	1985	3968507
hormone concentrations, mammary development and milk yield in goats given long-term bromocriptine treatment in pregnancy.	ten british saanen goats were treated daily with 5 mg bromocriptine intramuscularly from week 8 of pregnancy until week 20 (day 140). by comparison with untreated control goats (n = 8), concentrations of prolactin in plasma were suppressed throughout the treatment period and remained significantly lower until 3 days prepartum, parturition occurring on day 153 +/- 0.7 (mean +/- s.e.m., n = 10). growth hormone concentrations were low, but the incidence of levels exceeding 1 microgram/l was increas ...	1985	3968507
[changes in the rat aorta following ligation of the thoracic duct. ii. permeability studies].		1985	3974587