Publications

molecular diagnosis of fusobacterium necrophorum infection (lemierre's syndrome).	presented here is the case of a 27-year-old male with atypical features of lemierre's syndrome in which a definitive diagnosis was achieved using molecular methods. while routine investigations, including bacterial cultures, were unhelpful, two real-time pcr assays demonstrated fusobacterium necrophorum-specific dna in aspirates from brain and renal abscesses. this is the first report demonstrating that a laboratory diagnosis can be made using molecular methods in suspected cases of lemierre's s ...	2005	15776252
fusobacterium necrophorum-induced sepsis: an unusual case of lemierre's syndrome.	classical lemierre's syndrome is characterized by severe sepsis with metastatic abscess formation in young, previously fit people from a primary head or neck focus. the causative organisms are the anaerobic fusobacteria, most commonly fusobacterium necrophorum. we describe the evaluation, therapeutic interventions and management of a patient with lemierre's syndrome who presented in septic shock with multiple organ dysfunction. the patient required immediate interventions including endotracheal ...	2005	15777309
gross abnormalities, bacteriology and histological lesions of uteri of dairy cows failing to conceive or maintain pregnancy.	to describe the gross pathology, bacteriology and histopathology of the reproductive tracts of dairy cows that failed to conceive or maintain pregnancy.	2005	16044186
images in emergency medicine. lemierre syndrome.		2005	16046939
a cross-sectional study of the causes of morbidity and mortality in farmed white-tailed deer.	two questionnaires were designed and administered. the first was to a random sample of 340 farmers of white-tailed deer (odocoileus virginianus) in canada and the united states. the second was a 10-year retrospective survey of deer submissions to veterinary diagnostic pathology laboratories in canada and the united states. one-year rates of mortality and common causes of morbidity and mortality for the deer are reported. the primary diagnosis for each record was used to classify diseases into ca ...	2005	16048010
portal vein thrombosis despite anticoagulation in a person with diabetes.		2005	15805557
respiratory failure and hypercoagulability in a toddler with lemierre's syndrome.	a 3.5-year-old healthy boy with 4 days of fever was referred to the emergency department for respiratory distress. the physical examination was remarkable for stupor, tachycardia, tachypnea, and dyspnea. initial blood tests showed pancytopenia. he rapidly developed torticollis. computerized tomography of the neck revealed a thrombus in the internal jugular vein. a presumptive diagnosis of lemierre's syndrome was made and he was started on antibiotics and anticoagulation. he subsequently develope ...	2005	15833890
clindamycin-resistant fusobacterium varium bacteremia and decubitus ulcer infection.	bacteremia due to fusobacterium spp. is unusual (<10% of cases of anaerobic bacteremia), and the isolation of fusobacterium varium is especially uncommon. the most probable sources of fusobacterium bacteremia are the respiratory, the gastrointestinal, and the genitourinary tracts. a.-m. bourgault et al. (clin. infect. dis. 25[suppl. 2]:181-183) described 40 patients with fusobacterium bacteremia; only 3 had fusobacterium varium, and no one had decubitus scars as the portal of entry. in another p ...	2005	16082005
[efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study].	erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens.	2005	16087299
inhibitory effects of various micro-organisms on the growth of helicobacter pylori.	to examine the in vitro influence of various bacteria species on helicobacter pylori (hp) growth.	2005	15613007
genetic determinant of intrinsic quinolone resistance in fusobacterium canifelinum.	fourteen fluoroquinolone-resistant fusobacterial strains, originating from cats or dogs, were characterized by sequencing of the 16s-23s and 16s rrna genes and dna-dna hybridization and were described as a new species, fusobacterium canifelinum. all of the strains are intrinsically resistant (mic, >4 g/ml) to levofloxacin and other fluoroquinolones. compared to the quinolone resistance-determining region (gyra) of the susceptible relative f. nucleatum, we found that ser79 was replaced with leuci ...	2005	15616329
coaggregation profiles of the microflora from root surface caries lesions.	bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. as no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single candida spp. the quantitative coa ...	2005	15598414
periodontal infections and pre-term low birth weight: a case-control study.	pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ...	2005	15691348
fusobacterium nucleatum increases collagenase 3 production and migration of epithelial cells.	fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. initiation and outcome of infective diseases depend critically on the host cell signaling system altered by the microbe. production of proteinases by infected cells is an important factor in pericellular tissue destruction and cell migration. we studied binding of f. nucleatum to human epit ...	2005	15664960
fusobacterium necrophorum mediastinal abscess presenting as an anterior chest wall mass in a child: a case report.		2005	15678234
development and characterization of a simple perfused oral microcosm.	to validate perfused, inline, filter-based fermentation systems (multiple sorbarod devices, msd) for their ability to maintain stable oral bacterial communities. msd enable replicate (n=5) microcosm biofilms (bf) to be established and sampled, together with their perfusates (pa, cells in eluted medium).	2005	15715865
impact of sample storage on detection of periodontal bacteria.	information on the impact of sample storage prior to analysis by dna methods is limited. the aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration.	2005	15720575
quantitative detection of volatile sulfur compound- producing microorganisms in oral specimens using real-time pcr.	it is well-known that some periodontopathic bacteria, especially porphyromonas gingivalis, fusobacterium nucleatum, tannerella forsythia (formerly bacteroides forsythus or tan. forsythensis), and treponema denticola, actively produce volatile sulfur compounds (vscs), such as h2s and ch3sh. we previously reported a qualitative relationship between periodontopathic bacteria and vscs; however, a quantitative analysis of periodontopathic bacteria in oral specimens is required for further characteriz ...	2005	15752104
[pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology].	this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria.	2005	15757581
synergy between tannerella forsythia and fusobacterium nucleatum in biofilm formation.	during dental plaque formation, the interaction of different organisms is important in the development of complex communities. fusobacterium nucleatum is considered a 'bridge-organism' that facilitates colonization of other bacteria by coaggregation-mediated mechanisms and possibly by making the environment conducive for oxygen intolerant anaerobes. these studies were carried out to determine whether coaggregation between f. nucleatum and tannerella forsythia is important in the formation of mix ...	2005	15612944
anaerobic spondylodiscitis: case series and systematic review.	bacterial spondylodiscitis is rarely caused by anaerobic organisms. we describe two patients with lumbar vertebral osteomyelitis and discitis caused by anaerobic bacteria, including an unusual occurrence after an endodontic procedure, and review the salient clinical features and outcomes of 31 previously reported cases.	2005	15759942
bacteria recovered from dental pulp induce apoptosis of lymph node cells.	apoptosis is critical in the pathogenesis of several infectious diseases. the induction of apoptosis was assessed in mouse lymph node cells by four bacteria recovered from infected human dental pulp: gemella morbillorum, clostridium butyricum, fusobacterium nucleatum and bifidobacterium adolescentis. smaller lymph nodes and smaller numbers of cells were observed after experimental dental pulp infection with c. butyricum, suggesting that this bacterium induces cell death. apoptosis was evaluated ...	2005	15770029
fusobacteriosis presenting as community acquired pneumonia.	fusobacterium species are anaerobic gram-negative bacilli, which colonise the mucus membranes of man and animals and can cause a number of clinical manifestations including lemierre's disease (postanginal septicaemia), abdominal infection and deep-seated abscesses. the incidence of fusobacterium infections appears to be increasing, and we present three cases of fusobacteriosis who presented with features of community acquired pneumonia (cap). cases were treated with benzyl penicillin and metroni ...	2005	15780418
association of a novel high molecular weight, serine-rich protein (srpa) with fibril-mediated adhesion of the oral biofilm bacterium streptococcus cristatus.	the surface of the oral plaque bacterium streptococcus cristatus is decorated with a lateral tuft of fibrils. the fibrillar tuft functions in the adhesion of s. cristatus to heterologous bacterial species in the plaque biofilm. the tuft typically consists of a densely packed fringe of shorter fibrils 238 +/- 19 nm long with longer, less abundant fibrils 403 +/- 66 nm long projecting through the fringe of short fibrils. the two types of fibrils in the tufts of s. cristatus have been refractory to ...	2005	15836513
effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population.	the habit of chewing khat (catha edulis) for its amphetamine-like effects is highly prevalent in yemen and east africa, and has expanded to western countries. the purpose of this study was to estimate and compare the prevalence and levels of 14 periodontal bacteria in gingival plaque of khat chewers and khat nonchewers, as well as of khat chewing sides and khat nonchewing sides.	2005	15836514
detection of heat shock proteins but not superantigen by isolated oral bacteria from patients with behcet's disease.	we isolated bacteria from periodontal sites and mixed saliva in eight patients with behcet's disease and surveyed them to determine whether they produced heat shock proteins (hsps) and superantigen. cultivable bacterial compositions from periodontal sites and saliva were examined by anaerobic culture using blood agar plates. gram-negative anaerobic rods such as prevotella intermedia, fusobacterium nucleatum, and capnocytophaga species were predominant in the isolates from the subgingival plaque ...	2005	15836518
dna-dna hybridization demonstrates multiple bacteria in osteoradionecrosis.	bone necrosis secondary to radiation was previously attributed to trauma of devitalized bone and microbiological sepsis. however, conventional microbiological technique has failed to demonstrate microorganisms throughout osteoradionecrotic bone, claimed to be hypoxic, hypovascular and hypocellular. the aim of the present study was to examine such bone for bacteria using dna-dna hybridization. compared to standard culture methods this technique enables the investigation of a vast number of bacter ...	2005	15695050
oral microbiology and genomics.		2005	15853937
lemierre's syndrome: the controversy of anticoagulation.		2005	15867055
microbiology of acute and chronic maxillary sinusitis associated with an odontogenic origin.	: to study the microbiology of sinusitis associated with odontogenic origin.	2005	15867647
concentrations and in vivo antibacterial activity of spiramycin and metronidazole in patients with periodontitis treated with high-dose metronidazole and the spiramycin/metronidazole combination.	previous studies have shown that metronidazole, alone or in combination with spiramycin (250 mg/1 500 000 units, three times/day), is an effective treatment for active periodontitis, although the dose of metronidazole currently used (750 mg/day) could provide concentrations in gingival crevice fluid that are too low for the mics of the involved pathogens. this study tested the in vivo antibacterial efficacy of the currently used metronidazole dose (as contained in the fixed spiramycin/metronidaz ...	2005	15695539
tobacco smoking and periodontal microflora in a saudi arabian population.	to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits.	2005	15882210
susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells.	antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated.	2005	15886266
bacteriology of chronic maxillary sinusitis associated with nasal polyposis.	aspirates from 48 chronically inflamed maxillary sinuses from patients who had nasal polyposis were processed for aerobic and anaerobic bacteria. bacterial growth was present in 46 (96%) specimens. aerobic or facultative bacteria were present in 6 (13%) specimens, anaerobic bacteria alone in 18 (39%), and mixed aerobic and anaerobic bacteria in 22 (48%). there were 110 bacterial isolates (2.4 per specimen). thirty-nine of the isolates were aerobic or facultative organisms (0.85 per specimen). th ...	2005	15888469
clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle.	bacteria contaminate the uterus of most dairy cattle after parturition and endometritis causes infertility. an endometritis score can be ascribed based on the vaginal mucus character and odour but it is not clear if the clinical score reflects the number of uterine bacteria or the inflammatory response. the present study tested the hypothesis that clinical evaluation of endometritis reflects the number of bacteria present in the uterus, and the acute phase protein response. swabs (n = 328) were ...	2005	15589277
molecular analysis of microbial diversity in advanced caries.	real-time pcr analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. this suggests that an unresolved complexity exists in bacteria associated with advanced caries. in this report, the profile of the microflora of carious dentine was explored by using dna extracted from 10 lesions selected on the basis of comparable total microbial load and on the relative abundance of prevotella spp. using universal primers for t ...	2005	15695690
mucosa-associated bacteria in ulcerative colitis before and after antibiotic combination therapy.	we proposed that fusobacterium varium is one of the causative agents in ulcerative colitis.	2005	15813838
miriam hospital and rhode island hospital morbidity and mortality conferences, case of lemierre syndrome.		2005	15816248
towards a resolution on the inherent methodological weakness of the "effective number of codons used by a gene".	recently anders fuglsang provided a modified way for calculating n(c) when biased discrepancy is present in a gene [biochem. biophys. res. commun. 317 (2004) 957]. instead of taking the average codon homozygosity for each synonymous family type (as proposed by wright) [gene 87 (1990) 23] fuglsang considered codon homozygosity of each amino acid individually. marsashi and najafabadi [biochem. biophys. res. commun. 324 (2004) 1] in their recent article demonstrated that the readjustment for overes ...	2005	15823544
current approaches to the management of ovine footrot.	ovine footrot remains the most important cause of lameness in sheep flocks in the uk, despite the existence of proven methods for the control of the disease. recent research suggests that sheep farmers may be unaware of these methods and may allocate greater resources to treatment of footrot rather than to its prevention. foot paring, topical treatments, vaccination and parenteral antibiotic therapy all have a role in treating sheep with advanced footrot infections, but prevention of severe infe ...	2005	15683762
differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations.	this study intended to compare the prevalence of 7 putative endodontic pathogens in samples of primary endodontic infections taken from patients of 2 distant geographic locations.	2005	15829891
biodegradation of cyclic nitramines by tropical marine sediment bacteria.	undersea deposition of unexploded ordnance (uxo) constitutes a potential source of contamination of marine environments by hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (hmx). the goal of the present study was to determine microbial degradation of rdx and hmx in a tropical marine sediment sampled from a coastal uxo field in the region of oahu island in hawaii. sediment mixed cultures growing in marine broth 2216 (21 degrees c) anaerobically mi ...	2005	15915354
identification of cultivable microorganisms from primary endodontic infections with exposed and unexposed pulp space.	this study was aimed at comparing the cultivable microorganisms in canals with periapical radiolucencies with exposed and unexposed pulp space. microbiological samples were taken and analyzed from 45 canals with exposed pulp space, and 43 canals with unexposed pulp space. the canal contents were analyzed by aerobic/anaerobic culture, and conventional identification techniques. there were 211 isolates of bacteria belonging to 28 genera and 55 species recovered from exposed canals. in the unexpose ...	2005	15917680
periodontal pathogens: a quantitative comparison of anaerobic culture and real-time pcr.	periodontitis is a multi-factorial chronic inflammatory and destructive disease of the tooth-supporting tissues. quantitative anaerobic culture techniques have been used for microbial diagnosis of the different forms of the disease. the aim of this study was to compare real-time pcr with quantitative anaerobic culture for detection and quantification of 5 prominent periodontal pathogens. real-time pcr assays with the 16s rrna genes of actinobacillus actinomycetemcomitans, prevotella intermedia, ...	2005	15919188
molecular identification of an invasive gingival bacterial community.	a woman with neutropenia developed gingival hyperplasia. biopsy showed invasion of gingival tissue with mats of filamentous organisms, and molecular analysis by polymerase chain reaction and fluorescence in situ hybridization revealed capnocytophaga sputigena, leptotrichia species, and fusobacterium nucleatum. oral bacterial flora may cause invasive gingival disease with hyperplasia in immunocompromised patients.	2005	15937752
antimicrobial susceptibility variation of 50 anaerobic periopathogens in aggressive periodontitis: an interindividual variability study.	the frequent use of antibiotics in developed countries has led to the emergence of widespread bacterial resistance. in this study, the interindividual variability of the antibiotic susceptibility of 50 putative microorganisms in aggressive periodontitis patients has been evaluated by means of vc (variation coefficient).	2005	15943770
microarrays complement culture methods for identification of bacteria in endodontic infections.	the aim of this study was to investigate the microbial composition of necrotic root canals using culture methods and microarray technology. twenty uniradicular teeth with radiographic evidence of periapical bone loss and with no previous endodontic treatment were selected for this study. for molecular diagnosis a dna chip with 20 different species-specific, 16s-rdna-directed catcher probes was used. the microorganisms most frequently detected by the dna chip were: micromonas micros, fusobacteriu ...	2005	15943771
dna probe identification of bacteria colonizing internal surfaces of the implant-abutment interface: a preliminary study.	currently, there is limited knowledge concerning the specific genus and species of bacteria that may colonize internal surfaces of the implant-abutment interface (iai) of two-stage dental implants. the purpose of this study was to use dna probe analysis to identify those periodontopathic bacteria that may inhabit the internal surfaces and healing abutment screw-threads of the iai of dental implants in situ.	2005	15830645
clinicopathological conference: lemierre's syndrome.		2005	15692137
the role of anaerobic bacteria in tonsillitis.	this review summarizes the information that supports the potential importance of anaerobic bacteria in tonsillitis. some anaerobic bacteria possess interfering capability with group a beta-hemolytic streptococci (gabhs) and other pathogens. the possible role of anaerobes in the acute inflammatory process in the tonsils is supported by several observations: anaerobes have been isolated from the cores of tonsils of patients with recurrent gabhs and non-gabhs tonsillitis (nst); the recovery of anae ...	2005	15627441
[lemierre's syndrome and mycoplasma pneumoniae].	it has been suggested that a previous viral or bacterial pharyngitis may predispose to lemierre's syndrome, an uncommon entity nowadays. a case of lemierre's syndrome and co-infection with mycoplasma pneumoniae is described, association published before in only 2 other cases. a 16-year-old girl with trombophlebitis of the left jugular vein, fusobacterium necrophorum bacteremia and septic emboli in the lung requiring mechanical ventilation due to severe respiratory insufficiency. despite early tr ...	2005	16454590
the association of anaerobic bacteria with infectious mononucleosis.	this review summarizes the information that supports the potential importance of anaerobic bacteria in the pharyngo-tonsillitis (pt) associated with infectious mononucleosis (im). evidence supportive of the potential of involvement of bacteria in im was provided by several studies that showed increased recovery of bacteria on the tonsillar surfaces in im. several studies demonstrated that metronidazole therapy alleviated the clinical symptoms of tonsillar hypertrophy and shortened the duration o ...	2005	16701590
a case of lemierre's syndrome following epstein-barr virus infection.	lemierre's syndrome, or necrobacillosis, is a life-threatening septic thrombophlebitis of the internal jugular vein. the causative organism is fusobacterium necrophorum. here we report a case of lemierre's syndrome in a young male and describe the clinical presentations and treatment. epstein-barr virus (ebv) was a suspected predisposing factor in this case.	2005	16701549
analysis of the antibacterial activity and plaque control benefit of colgate total dentifrice via clinical evaluation and real-time polymerase chain reaction.	this study analyzed, from a combined clinical and molecular biologic perspective, the antibacterial and antiplaque efficacy of colgate total dentifrice (ctd).	2005	16583596
sodium ion pumps and hydrogen production in glutamate fermenting anaerobic bacteria.	anaerobic bacteria ferment glutamate via two different pathways to ammonia, carbon dioxide, acetate, butyrate and molecular hydrogen. the coenzyme b12-dependent pathway in clostridium tetanomorphum via 3-methylaspartate involves pyruvate:ferredoxin oxidoreductase and a novel enzyme, a membrane-bound nadh:ferredoxin oxidoreductase. the flavin- and iron-sulfur-containing enzyme probably uses the energy difference between reduced ferredoxin and nadh to generate an electrochemical na+ gradient, whic ...	2005	16645308
lemierre's syndrome.	lemierre's syndrome is a rare, potentially severe disease consisting of septic emboli from an internal jugular vein thrombus after oropharyngeal infection. we report a 20-year-old man who had a tonsillitis with fever for 3 days. after a 2-day defervescence, fever relapsed as well as painful swelling over the left side of the neck. computed tomography scan and sonography of the neck revealed thrombosis of left internal jugular vein and swelling of surrounding soft tissue. chest radiograph demonst ...	2005	16385382
effectiveness of antibiotic combination therapy in patients with active ulcerative colitis: a randomized, controlled pilot trial with long-term follow-up.	it is proposed that fusobacterium varium might be one of the elusive pathogenic factors in ulcerative colitis (uc). our goal was to assess whether an antibiotic combination therapy against f. varium is effective for induction and maintenance of remission of uc.	2005	16334443
pyogenic liver abscess after colonoscopy in a patient with ulcerative colitis.		2005	16361038
efficacy of vaccination against fusobacterium necrophorum infection for control of liver abscesses and footrot in feedlot cattle in western canada.	a randomized and blinded field trial was carried out to evaluate the efficacy of a fusobacterium necrophorum bacterin for control of liver abscesses and footrot under commercial feedlot conditions in western canada. half of the vaccinated and half of the unvaccinated control animals had ad libitum access to a forage-based (alf) growing diet. the other half of each group was limit-fed a grain-based (lfg) growing diet. the overall prevalence of a and a+ liver abscesses in this trial was 16.7%. a s ...	2005	16363327
[anaerobic bacteria isolated from patients with suspected anaerobic infections].	the study involved 394 clinical samples sent to the clinical microbiology laboratory of hacettepe university adult hospital between january 1997 and may 2004 for anaerobic cultivation. since multiple cultures from the same clinical samples of the same patient were excluded, the study was carried on 367 samples. the anaerobic cultures were performed in anaerobic jar using anaerogen kits (oxoid, basingstoke, u.k.) or genbox (biomérieux, lyon, france). the isolates were identified by both classical ...	2005	16544546
microbial diversity in coastal subsurface sediments: a cultivation approach using various electron acceptors and substrate gradients.	microbial communities in coastal subsurface sediments are scarcely investigated and have escaped attention so far. but since they are likely to play an important role in biogeochemical cycles, knowledge of their composition and ecological adaptations is important. microbial communities in tidal sediments were investigated along the geochemical gradients from the surface down to a depth of 5.5 m. most-probable-number (mpn) series were prepared with a variety of different carbon substrates, each a ...	2005	16332756
lemierre's syndrome: the link between a simple sore throat, sore neck and pleuritic chest pain.	an unusual case of tonsillitis which showed progression to this rare syndrome despite treatment with intravenous antibiotics. lemierre's syndrome is a rare condition characterised by a triad of: sepsis, thrombophlebitis of the internal jugular vein along with pleuropulmonary and/or distant metastatic abscesses. diagnosis rests on a high index of suspicion and is confirmed by culture of fusobacterium spp. from blood or infected sites. radiological investigations are established aids for confirmat ...	2005	16053693
[empyema caused by fusobacterium necrophorum].		2005	16302307
infected pneumatocele following anaerobic pneumonia in adult.	we report a case of an infected pneumatocele in the course of anaerobic pneumonia in an adult. to the best of our knowledge, anaerobic pneumonia complicated by a pneumatocele in an adult has not previously been described. the pneumatocele occurred on the fifth day of hospitalization, and rapidly increased in size, with the development of a subsequent mixed anaerobe infection. a pig-tail catheter was inserted and the pus drained. the bacterial culture from the pus was positive for three anaerobes ...	2005	16491835
universal dna primers amplify bacterial dna from human fetal membranes and link fusobacterium nucleatum with prolonged preterm membrane rupture.	a large number of bacterial species have been identified in fetal membranes after preterm labour (ptl) associated with intrauterine infection by microbiological culture. in this study, we have investigated a molecular and bioinformatic approach to organism identification which surmounts the need for specific and diverse microbiological culture conditions required by conventional methods. samples of fetal membranes were taken from 37 preterm infants, and 6 normal term controls delivered by caesar ...	2005	16254004
clinical and microbiological characterization of periodontal abscesses.	the knowledge of clinical features, microbial composition and susceptibility to antimicrobials of periodontal abscesses has recently improved. this descriptive clinical and microbiological study provides more information on the characteristics of periodontal abscesses.	2005	16268997
coaggregation ability of weissella cibaria isolates with fusobacterium nucleatum and their adhesiveness to epithelial cells.	in the present study, we evaluated the ability of weissella cibaria isolated from the oral cavity to coaggregate with fusobacterium nucleatum, and the adhesiveness of these strains to epithelial cells. w. cibaria efficiently coaggregated with f. nucleatum, and adhered to epithelial cells. we tested the effects of various factors on the coaggregation. the coaggregation and adhesiveness of w. cibaria disappeared upon exposure to pronase or licl, suggesting that proteinaceous components on the surf ...	2005	16288835
fusobacterium necrophorum infections in animals: pathogenesis and pathogenic mechanisms.	fusobacterium necrophorum, a gram-negative, non-spore-forming anaerobe, is a normal inhabitant of the alimentary tract of animals and humans. two subspecies of f. necrophorum, subsp. necrophorum (biotype a) and subsp. funduliforme (biotype b), have been recognized, that differ morphologically, biochemically, and biologically. the subsp. necrophorum is more virulent and is isolated more frequently from infections than the subsp. funduliforme. the organism is an opportunistic pathogen that causes ...	2005	16701574
enrichment of fusobacteria from the rumen that can utilize lysine as an energy source for growth.	ruminal lysine degradation is a wasteful process that deprives the animal of an essential amino acid. mixed ruminal bacteria did not deaminate lysine (50 mm) at a rapid rate, but lysine degrading bacteria could be enriched if trypticase (5 mg/ml) was also added. lysine degrading isolates produced acetate, butyrate and ammonia, were non-motile, stained gram-negative and could also utilize lactate, glucose, maltose or galactose as an energy source for growth. lactate was converted to acetate and p ...	2005	16701548
anaerobes in the upper respiratory tract in infancy.	development of the indigenous microbiota begins on the surfaces of the human body after birth when infants are exposed to continuous person-to-person and environmental contacts with microbes. anaerobes constitute a significant part of indigenous bacterial communities at different body sites. pioneering anaerobic commensals are able to colonize and survive in the oral cavity during the first months of life. after teeth emerge, more attachment sites and potential niches are available for anaerobic ...	2005	16701543
the leukotoxin operon of fusobacterium necrophorum is not present in other species of fusobacterium.	the complete nucleotide sequence of the leukotoxin operon of fusobacterium necrophorum has been determined. the operon consists of three genes (lktbac) of which the leukotoxin structural gene is the middle determinant. southern and western blot analyses and flow cytometry analysis for biological activity of the culture supernatants were carried out to determine if the leukotoxin is present in other species of the genus fusobacterium. only the two subspecies of f. necrophorum were found to posses ...	2005	16701542
antimicrobial effect in vitro of chlorhexidine and calcium hydroxide impregnated gutta-percha points.	the aim of this study was to evaluate the antimicrobial activity of points containing antiseptics used for temporary obturation in endodontics. points containing calcium hydroxide (roeko and hygienic), and chlorhexidine (roeko) were tested and gutta-percha points served as control (meta dental corp.). the following microorganisms were studied: streptococcus mutans (sm), enterococcus faecalis (ef), staphylococcus aureus (sta), candida albicans (ca), fusobacterium nucleatum (fn) atcc 25586, porphy ...	2005	16673792
subdoligranulum variabile gen. nov., sp. nov. from human feces.	during studies on the microflora of human feces we have isolated a strictly anaerobic, non-spore-forming, gram-negative staining organism which exhibits a somewhat variable coccus-shaped morphology. comparative 16s ribosomal rna gene sequencing studies show the unidentified organism is phylogenetically a member of the clostridium leptum supra-generic rrna cluster and displays a close affinity to some rdna clones derived from human and pig feces. the nearest named relatives of the unidentified is ...	2004	16701519
anaerobic microbiology in 198 cases of pleural empyema: a bulgarian study.	the aim of the study was to evaluate the incidence of anaerobic bacteria in 198 patients with pleural empyema and the susceptibility of isolates to eight antibacterial agents. isolates were identified by the crystal anaerobes identification system, api system rapid id 32 a and/or routine methods. susceptibility was tested by sceptor mic system for anaerobic bacteria and limited agar dilution method. anaerobic bacteria were found in 74.2% of the patients and included 247 strains within 21 genera. ...	2004	16701526
'papillomatization' and 'malignant transformation' of an abscess cavity in a 25-year old man with recurrent respiratory papillomatosis who may have had lemierre syndrome.	we report here an unusual case of a 25-year old male with juvenile onset recurrent respiratory papillomatosis who developed an infestation of papillomata 'papillomatization' of an old abscess cavity that, when removed, was coincidently found to contain a 2 cm well differentiated squamous cell carcinoma. the abscess cavity had been caused by a previous fusobaterium necrophorum infection that probably developed as a result of septic emboli from his internal jugular veins. this once common but now ...	2004	16281650
[a severe throat infection - case report.]	we report a case of lemierre?s syndrome or, human necrobacillosis, in a 33 year old icelandic male. a severe clinical picture, fulfilling all the criteria for this syndrome, is described. with antibiotic therapy, and critical care, the outcome was successful. a brief overview of infections due to fusobacterium necro phorum, with special emphasis on lemierre?s syndrome, is presented. etiology, clinical symptoms, treatment and prognosis of this syndrome are dis cussed. we believe this to be the fi ...	2004	16819062
lemierre's syndrome: case report in a pediatric patient.	lemierre's syndrome was described in 1936 as a severe oropharyngeal infection followed by septic thrombophlebitis of the internal jugular vein and disseminated metastatic infections. cases occur typically in previously healthy young adults and children. fusobacterium necrophorum is the main anaerobic bacterium implicated. we present a septic 2-month-old infant with mastoiditis, multiple sites of osteoarthritis and multiple subcutaneous abscesses. no underlying anatomic or immunologic abnormaliti ...	2004	16701512
occurrence of candida strains in cases of paronychia.	a total of 43 patients, 11 males and 32 females, with paronychia of the fingernails were examined for the presence of candida spp. the yeast species isolated were identified using standard laboratory methods, including germ-tube production, morphology on rice agar with tween 80, and mainly fermentation and assimilation of saccharides. in the male group, two candida species were detected: c. albicans as the dominant species in 9 patients and c. parapsilosis in 2 cases. similarly, c. albicans was ...	2004	15702551
[liver abscesses secondary to sigmoid diverticulitis: report of four cases].	pyogenic liver abscess often revealed by right sided abdominal pain and fever is a serious and life-threatening pathology. biliary tract disease is the origin of the abscess in most cases but sometimes remains unidentified. a sigmoid septic source sometimes paucisymptomatic or hidden by an immunosuppressive treatment must be looked for.	2004	15582171
effectiveness of a triclosan/copolymer dentifrice on microbiological and inflammatory parameters.	according to the us surgeon general's report, "oral health in america," published in 2000, most adults in the united states show some degree of periodontal pathology, with severe periodontal diseases affecting about 14% of middle-aged adults. periodontal diseases are polymicrobial-induced inflammatory diseases, and they vary from mild gingival inflammation to severe deterioration of the periodontium, ie, loss of periodontal supportive tissues and, ultimately, tooth loss. new evidence shows that ...	2004	15645886
lemierre's syndrome: three cases and a review.	postanginal sepsis, also called lemierre's syndrome, is a rare but acute medical condition complicating oropharyngeal infection. the pathogenesis consists of the development of internal jugular vein septic thrombophlebitis leading to metastatic infections in the lung and other sites. the causative microorganism is most often fusobacterium necrophorum. we present three patients with lemierre's syndrome who presented to the leiden university medical center and give a review of the literature.	2004	15599753
[lemierre syndrome: a diagnosis to keep in mind].	lemierre syndrome (also called post-anginal sepsis or necrobacillosis) is an uncommon complication of oropharyngeal infections for which early recognition and aggressive therapy is mandatory, since it is potentially life-threatening. due to it's rarity, with a prevalence of 0.8 cases per million in the general population, many physicians are unfamiliar with this disorder. this is attested by the fact that diagnosis most often relies on bacteriological grounds. we report the case of a patient who ...	2004	15631168
the aetiology and treatment of oral halitosis: an update.	halitosis refers to the condition of offensive mouth odour. more than 90% of cases of halitosis originate from the oral cavity. the implicated bacteria (fusobacterium nucleatum, prevotella intermedia, and tannerella forsythensis) are located in stagnant areas in the oral cavity, such as the dorsal surface of tongue, periodontal pockets, and interproximal areas. these bacteria proteolyse the amino acids releasing volatile sulphur compounds. the management of halitosis involves determining and eli ...	2004	15591601
attachment of oral gram-negative anaerobic rods to a smooth titanium surface: an electron microscopy study.	attachment of bacteria to titanium may differ not only between bacterial species but also between strains within a species. the aim of the present in vitro study was to examine differences in bacterial attachment using 4 gram-negative anaerobic species of bacteria that are considered potential periodontal pathogens.	2004	15623054
differential regulation of cytokine genes in gingival epithelial cells challenged by fusobacterium nucleatum and porphyromonas gingivalis.	il-8 mrna in human gingival epithelial cells (hgecs) is up-regulated by fusobacterium nucleatum, and up-/down-regulated by porphyromonas gingivalis in a complex interaction in the early stages (< or = 4 h) after infection. the mechanisms involved in this regulation in response to f. nucleatum and/or p. gingivalis infection, and identification of co-regulated cytokine genes, are the focus of this investigation. heat, formalin or protease treatment of f. nucleatum cells attenuated the il-8 mrna up ...	2004	15619426
characterization of the novel fusobacterium nucleatum plasmid pkh9 and evidence of an addiction system.	fusobacterium nucleatum is an important oral anaerobic pathogen involved in periodontal and systemic infections. studies of the molecular mechanisms involved in fusobacterial virulence and adhesion have been limited by lack of systems for efficient genetic manipulation. plasmids were isolated from eight strains of f. nucleatum. the smallest plasmid, pkh9 (4,975 bp), was characterized and used to create new vectors for fusobacterial genetic manipulation. dna sequence analysis of pkh9 revealed an ...	2004	15574887
simultaneous detection of dialister pneumosintes and filifactor alocis in endodontic infections by 16s rdna-directed multiplex pcr.	dialister pneumosintes and filifactor alocis have been recently considered as candidate endodontic pathogens. in this study, we devised a 16s rdna-directed multiplex pcr protocol for simultaneous detection of these two bacterial species in endodontic infections. samples were taken from infected root canals associated with asymptomatic periradicular lesions as well as from cases of acute periradicular abscesses. dna extracted from the samples was used as template for simultaneous detection of d. ...	2004	15564862
investigation of the effect of the coronal restoration quality on the composition of the root canal microflora in teeth with apical periodontitis by means of t-rflp analysis.	to investigate the effect of the radiographic and clinical quality of coronal restorations on the composition of the root canal flora of teeth with necrotic pulps and teeth with root fillings associated with apical periodontitis.	2004	15548272
[an unusual case of hepatic abscess].	right upper quadrant pain with chronic malaise and fever can be the clinical manifestation of a hepatic infection. an uncommon cause for the disease is hepatic actinomycosis. actinomycosis was common in the preantibiotic era but is less frequent nowadays; consequently its timely recognition has become more difficult. the clinical and radiological findings often resemble other inflammatory and neoplastic lesions. we report a case of a mixed anaerobic liver abscess including fusobacteria and actin ...	2004	15553899
single species biofilm-forming ability of root canal isolates on gutta-percha points.	the participation of bacterial biofilms in the over-filled gutta-percha points associated with refractory periapical periodontitis has recently been reported. this study investigated the initial biofilm-forming ability of root canal isolates (enterococcus faecalis, streptococcus sanguis, strep. intermedius, strep. pyogenes, staphylococcus aureus, fusobacterium nucleatum, propionibacterium acnes, porphyromonas gingivalis and prevotella intermedia) on gutta-percha points in vitro. each bacterial s ...	2004	15560836
phylogenetic analysis of fusobacterium necrophorum, fusobacterium varium and fusobacterium nucleatum based on gyrb gene sequences.	the nucleotide sequences of the dna gyrase b subunit gene (gyrb) of fusobacterium necrophorum subsp. necrophorum, f. necrophorum subsp. funduliforme and f. varium were determined and analyzed together with those of f. nucleatum subsp. nucleatum and f. nucleatum subsp. vincentii. on the phylogenetic tree constructed, the strains of each fusobacterial species formed distinct clusters with deep sublines. the degree of sequence similarity within each cluster was 93.2% or more, whereas similarities b ...	2004	15528856
a young woman with urinary symptoms, septicaemia and a pectoral muscle abscess.		2004	15449495
antiproliferative activity of actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum in peripheral blood mononuclear cells.	several studies indicate actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum as etiologic agents of periodontal disease. immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. this study evaluated the antiproliferative activity of ammonium precipitate fractions of a. (h.) actinomycetemcomitans and f. nucleatum isolates from humans and marmosets both with and without periodontal disease. all a. (h.) acti ...	2004	15501650
in situ biofilm formation by multi-species oral bacteria under flowing and anaerobic conditions.	an understanding of biofilm behavior of periodontopathic bacteria is key to the development of effective oral therapies. we hypothesized that interspecies bacterial aggregates play an important role in anaerobic biofilm establishment and proliferation, and contribute to the survivability of the biofilm against therapeutic agents. the system developed in this study assessed a multi-species (streptococcus gordonii, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum) biofilm formatio ...	2004	15381723
brain abscess after milk tooth self-extraction.	brain abscesses are rare, especially in children, but they can be life-threatening infections. to date, dental pathology has been linked to only a small number of brain abscesses. to our best knowledge this is the first reported case of a brain abscess following self-extraction of a milk tooth. we are reporting on a 12-year-old previously healthy boy who developed a brain abscess in the vicinity of the left precentral gyrus. clinical examination prior to surgery showed a severe right hemiparesis ...	2004	15506321
cavernous sinus thrombosis and cerebral infarction caused by fusobacterium nucleatum infection.	we report an unusual case of fusobacterial infection with secondary intracranial invasion. the condition was complicated by a cavernous sinus thrombosis and ischemic stroke. the patient was a 63-year-old woman with no history of systemic disease who had undergone a tooth extraction before the onset of symptoms. she initially suffered from sphenomaxillary sinusitis and a cavernous sinus thrombosis, and subsequently developed meningitis. cerebrospinal fluid examination suggested a pyogenic infecti ...	2004	15455548
[polymicrobial sepsis and perityphlic abscess: case report and review of literature].	to evaluate the safety and efficacy of conservative therapy of polymicrobial anaerobic sepsis and appendiceal mass.	2004	15700692
direct detection of bacterial pathogens in brain abscesses by polymerase chain reaction amplification and sequencing of partial 16s ribosomal deoxyribonucleic acid fragments.	to evaluate the feasibility of detecting bacterial pathogens directly from the clinical brain abscess specimens by polymerase chain reaction (pcr) amplification and sequencing of bacterial 16s ribosomal deoxyribonucleic acid (rdna).	2004	15509322
anaerobic pulmonary infections in children.	pulmonary infections due to anaerobic bacteria usually occur in children prone to aspiration. the source of the anaerobic bacteria is the oropharyngeal bacterial flora, where these organisms outnumber aerobic and facultative organisms in a 10:1 ratio. the most common lower respiratory tract infections where anaerobic bacteria are recovered mixed with aerobic organisms are aspiration pneumonia, lung abscess, and empyema. the predominant isolated anaerobic bacteria are peptostreptococcus, fusobact ...	2004	15599270
real-time pcr investigation into the importance of fusobacterium necrophorum as a cause of acute pharyngitis in general practice.	fusobacterium necrophorum is recognized as the cause of a severe life-threatening illness characterized by bacteraemia with metastatic abscesses following an acute sore throat (lemierre's disease). however, the importance of f. necrophorum as a cause of simple sore throat in the community is unknown. using quantitative real-time pcr with primers targeting the rpob gene, 100 routine throat swabs collected from patients presenting to general practitioners with pharyngitis were analysed for the pre ...	2004	15358827