Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| health-care marketing: not for large institutions only. | 1998 | 108194 | |
| an innovative approach to surveillance of nosocomial outbreaks. | 1998 | 121403 | |
| [heredity in eye diseases. ii. foundations of ophthalmological genetics (author's transl)]. | 1998 | 108459 | |
| [effectiveness of prolonged uterine relaxation by betamimetic drugs for threatened premature labour (author's transl)]. | it is statistically proven that long-term administered betamimetic drugs inhibit excessive uterine activity, but for the individual subject the extent of this effect can hardly be predicted. by studying 167 pregnancies treated for threatened premature labour it is confirmed that the success rate of the tocolytic treatment depends strictly on the clinical feature of the condition as observed on admission. by use of a simple scheme for classification of success, correlations with the importance of ... | 1998 | 105481 |
| [comparison of 24 hours ambulatory electrocardiography and endocavitary recording in the diagnosis of heart rate disorders]. | ninety symptomatic patients aged between 16 and 90 years were investigated by ambulatory continuous 24 hour electrocardiography. 75 of these patients underwent endocavitary exploration of atrioventricular conduction and sinus node function within 48 hour of ambulatory electrocardiography. symptoms occurred during the recording in 30% patients, enabling the mechanism of the malaise to be determined. every time that abnormalities in the zone surrounding the tawara node were demonstrated by endocav ... | 1998 | 106788 |
| double phakomatosis. | in the phakomatoses--von recklinghausen's disease, the sturge-weber syndrome, tuberous sclerosis, and von hippel-lindau disease--pathologic changes in the eye are often evident. most cases are described as a single entity. the following is a case report of concomitant sturge-weber syndrome and von recklinghausen's disease in which both conditions involved the eye. | 1998 | 105694 |
| clinical evaluation of warthin's tumor. an analysis of 43 cases. | forty-three patients with warthin's tumor (26 males and 17 females) were analyzed and the following results were obtained: all tumors were related to the parotid gland with no bilateral involvements. the ages of the patients were 20-84 years old and the average age was about 62. the interval between tumor recognition and first examination was 1 week to 16 years with the average interval of 20-22 months. the method of treatment was complete enucleation. however, radiation therapy was also done in ... | 1998 | 107138 |
| how the nurse copes with grief. care of the child with cancer. | 1998 | 107941 | |
| survey indicates adults flout advice in matters of health. | 1998 | 105641 | |
| neurofibromatosis: unusual lymphangiographic findings. | 1998 | 106665 | |
| molecular biology to check patients treated for cml. | 1997 | 2627627 | |
| world medical assembly, dublin, 1977. | 1997 | 23488 | |
| transcriptional regulation of the glta and tlc genes in rickettsia prowazekii growing in a respiration-deficient host cell. | the regulation of the citrate synthase (glta) and atp/adp translocase (tlc) genes of the obligate intracellular bacterium, rickettsia prowazekii, was analyzed in rickettsia-infected respiration-deficient g14 cells. the level of the glta mrnaii and the tlc mrna was much lower in the total rna isolated from the infected g14 cells grown in 1 g/l glucose (low glucose, gl) medium than in that from infected g14 cells grown in 4.5 g/l glucose (high glucose, gh) medium. however, the level of the glta mr ... | 1997 | 9607082 |
| [a prognostic assessment of louse-borne typhus (rickettsia prowazekii infection) in russia]. | from the 1950s to 1974 r.prowazekii infection was registered on the territory of russia, mainly in the form of brill's disease, represented by some individual cases in the focus of infection. in 0.25% of all of cases rickettsiosis was linked with group outbreaks of the family character (14 events), reflecting the epidemic form of the disease. from 1974 until the present time the correlation between the sources of infection and the infestation of the population with lice dropped below the critica ... | 1997 | 9460861 |
| increased and controlled expression of the rickettsia prowazekii atp/adp translocase and analysis of cysteine-less mutant translocase. | detailed molecular analysis of the rickettsia prowazekii atp/adp translocase, an obligate exchange transport system that is specific for atp and adp, has been extremely difficult due to limited quantities of material available from these obligate intracytoplasmic bacteria and by the toxicity and poor expression in recombinant escherichia coli expression systems. in this study, a stable and controllable system for the increased expression of the rickettsial atp/adp translocase was developed in e. ... | 1997 | 9387243 |
| domain organization of escherichia coli transcript cleavage factors grea and greb. | the grea and greb proteins of escherichia coli induce cleavage of the nascent transcript in ternary elongation complexes of rna polymerase. gre factors are presumed to have two biologically important and evolutionarily conserved functions: the suppression of elongation arrest and the enhancement of transcription fidelity. a three-dimensional structure of greb was generated by homology modeling on the basis of the known crystal structure of grea. both factors display similar overall architecture ... | 1997 | 9054416 |
| citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae. | using pcr and an automated laser fluorescent dna sequencer, we amplified and sequenced a 1,234-bp fragment of the citrate synthase-encoding gene (glta) of 28 bacteria belonging to the genus rickettsia. comparative sequence analysis showed that most of the spotted fever group (sfg) rickettsiae belonged to one of two subgroups. the first subgroup included rickettsia massiliae, strain bar 29, rickettsia rhipicephali, "rickettsia aeschlimanni," and rickettsia montana, which have been isolated only f ... | 1997 | 9103608 |
| identification of grea encoding a transcriptional elongation factor as a member of the cara-orf-carb-grea operon in pseudomonas aeruginosa pao1. | a homolog of the transcriptional elongation factor, grea, was identified in pseudomonas aeruginosa pao1. the deduced amino acid sequence for grea from this organism exhibits 65.2% identity to its counterpart in escherichia coli k-12. the nucleotide sequence of grea from p. aeruginosa overlaps by four bases the 3' terminus of carb which encodes the large subunit of carbamoylphosphate synthetase. s1 nuclease experiments showed that level of the grea transcript is elevated approximately 10-fold und ... | 1997 | 9139926 |
| epidemic typhus in a prison in burundi. | the international committee of the red cross investigated an outbreak of fever of unknown origin in ngozi prison, burundi, which resulted in a crude mortality rate of 2.61% in january 1996. a definite diagnosis of epidemic typhus caused by rickettsia prowazekii was established by enzyme-linked immunosorbent assay using specific antigens. control measures included complete cleansing of the prison with cyfluthrine, shaving and dusting all prisoners with permethrin 0.5% dusting powder, and replacem ... | 1997 | 9196749 |
| [the use of a method of estimating the count of viable rickettsia prowazekii for the screening of efficacious antibiotics]. | the possibility of the evaluation of antirickettsial activity of chemopreparations, both in vitro (by the inhibition of hemolytic activity) and in vivo (under the conditions of experimental pulmonary rickettsiosis in mice), with the use of the rapid method for the determination of the number of metabolically active rickettsiae is shown. the proposed approach may be used in the complex of mutually complementary methods for the screening of antirickettsial preparations. | 1997 | 9245137 |
| genomic rearrangements during evolution of the obligate intracellular parasite rickettsia prowazekii as inferred from an analysis of 52015 bp nucleotide sequence. | in this study a description is given of the sequence and analysis of 52 kb from the 1.1 mb genome of rickettsia prowazekii, a member of the alpha-proteobacteria. an investigation was made of nucleotide frequencies and amino acid composition patterns of 41 coding sequences, distributed in 10 genomic contigs, of which 32 were found to have putative homologues in the public databases. overall, the coding content of the individual contigs ranged from 59 to 97%, with a mean of 81%. the genes putative ... | 1997 | 9274032 |
| [rickettsiosis]. | 1997 | 9278031 | |
| jail fever (epidemic typhus) outbreak in burundi. | we recently investigated a suspected outbreak of epidemic typhus in a jail in burundi. we tested sera of nine patients by microimmunofluorescence for antibodies to rickettsia prowazekii and rickettsia typhi. we also amplified and sequenced from lice gene portions specific for two r. prowazekii proteins: the gene encoding for citrate synthase and the gene encoding for the rickettsial outer membrane protein. all patients exhibited antibodies specific for r. prowazekii. specific gene sequences were ... | 1997 | 9284381 |
| transcriptional characterization of the rickettsia prowazekii major macromolecular synthesis operon. | recent studies have demonstrated that rickettsia prowazekii can regulate transcription of selected genes at the level of initiation. however, little information concerning the existence of operons and coordinate gene regulation in this obligate intracellular parasitic bacterium is available. to address these issues, we have focused on the rpod gene linkage group (grea-open reading frame 23 [orf23]-dnag-rpod), which includes the rickettsial analog (orf23-dnag-rpod) of the major macromolecular syn ... | 1997 | 9335295 |
| immunohistochemical diagnosis of typhus rickettsioses using an anti-lipopolysaccharide monoclonal antibody. | a monoclonal antibody directed against an epitope on the lipopolysaccharide of typhus-group rickettsiae was developed for the purpose of detecting this heat-stable, proteinase-resistant antigen in formalin-fixed, paraffin-embedded tissues. rickettsia prowazekii organisms were identified in endothelium and macrophages in sections of the brains of three egyptian men who died of epidemic louse-borne typhus in cairo during world war ii and in the brain from a recent case of typhus fever acquired in ... | 1997 | 9346184 |
| ninth international genome sequencing and analysis conference. hilton head island, south carolina. september 13-16, 1997. | 1997 | 9689223 | |
| [current problems in the laboratory control of louse-borne typhus infection in ukraine]. | surveys designed to study spread of typhus infection (persistent form and brill's disease), and state of laboratory diagnosis in ukraine showed an important role the laboratory diagnosis plays in the system of epidemiological surveillance aimed at preventing epidemic typhus. main trends of activities on perfection of the system of laboratory control of the infection under present-day conditions are outlined. | 1996 | 9138778 |
| how charles nicolle of the pasteur institute discovered that epidemic typhus is transmitted by lice: reminiscences from my years at the pasteur institute in paris. | 1996 | 8855211 | |
| the rickettsia prowazekii genome: a random sequence analysis. | we describe here the first general survey of the genomic content and the coding capacity of the 1.1 mb genome of rickettsia prowazekii based on an analysis of a total of 200 kb of unique sequence data collected in a random manner. based on nucleotide distribution profiles, we estimate that the r. prowazekii genome may have a coding density of 60%-70% and that it may contain a total of circa 800 genes. here, we have tentatively identified and classified 173 of these genes. our analysis suggests t ... | 1996 | 9689214 |
| identification and characterization of epitopes on the 120-kilodalton surface protein antigen of rickettsia prowazekii with synthetic peptides. | the 120-kda surface protein antigens (spas) of typhus rickettsiae are highly immunogenic and have been shown to be responsible for the species-specific serological reactions of the typhus group rickettsiae. to study the immunochemistry of these proteins, overlapping decapeptides encompassing the whole protein were synthesized on derivatized polyethylene pins. a modified enzyme-linked immunosorbent assay was used to identify epitopes recognized by rabbit hyperimmune antisera to rickettsia prowaze ... | 1996 | 8606109 |
| transcriptional analysis of the 16s rrna gene in rickettsia prowazekii. | the control of rrna synthesis in the etiological agent of epidemic typhus, rickettsia prowazekii, a slowly growing obligate intracytoplasmic bacterium, was investigated. transcription of the rickettsial 16s rrna gene (rrs), of which there is only a single copy, was controlled by a single promoter region, and the site for the initiation of transcription (base a) was found 117 bp upstream of the rrs coding region for the mature product. the promoter region contained an escherichia coli promoter-li ... | 1996 | 8626305 |
| codon usage and base composition in rickettsia prowazekii. | codon usage and base composition in sequences from the a + t-rich genome of rickettsia prowazekii, a member of the alpha proteobacteria, have been investigated. synonymous codon usage patterns are roughly similar among genes, even though the data set includes genes expected to be expressed at very different levels, indicating that translational selection has been ineffective in this species. however, multivariate statistical analysis differentiates genes according to their g + c contents at the ... | 1996 | 8662004 |
| amino acid sequence alignment of a "small' citrate synthase from pseudomonas aeruginosa pac 514, with other citrate synthase sequences. | 1996 | 8674717 | |
| the typhus group. | 1996 | 8727129 | |
| [spotted fever group rickettsiosis and vectors in kanagawa prefecture]. | primer pairs for pcr were designed from the gene encoding the 17,000-molecular-weight genus-common antigen of rickettsia japonica, rickettsia rickettsii, rickettsia conorii, rickettsia typhi and rickettsia prowazekii. primers r1, r2 were designed for amplifying the genomic dna from spotted fever group (sfg) rickettsiae and epidemic typhus rickettsiae. primers rj5, rj10 were designed for amplifying the genomic dna from only r. japonica. using the primers r1, r2, about a 540-bp fragment was observ ... | 1996 | 8741704 |
| the biology of rickettsiae. | rickettsiae are bacterial obligate intracellular parasites ranging from harmless endosymbionts to the etiologic agents of some of the most devastating diseases known to mankind. rickettsiae are primarily associated with arthropod vectors in which they may exist commensally and, in most cases, only accidentally infect humans. these fascinating microbes are the prototypical obligate intracellular parasites. other than being extremely fastidious in their growth requirements, however, rickettsiae ar ... | 1996 | 8805076 |
| transcriptional regulation in the obligate intracytoplasmic bacterium rickettsia prowazekii. | transcriptional regulation was demonstrated in rickettsia prowazekii, an obligate intracytoplasmic bacterium. the level of citrate synthase (glta) mrna ii, from promoter p2, was greater in the total rna isolated from heavily infected l929 cells than in moderately infected l929 cells; conversely, the level of atp/adp translocase (tlc) mrna was greater in moderately infected cells. the level of glta mrna i, from promoter p1, did not change under these conditions. the chemical half-lives of glta mr ... | 1996 | 8808950 |
| improved plaque assays for rickettsia prowazekii in vero 76 cells. | typhus group rickettsiae, including rickettsia prowazekii and r. typhi, produce visible plaques on primary chick embryo fibroblasts and low-passage mouse embryo fibroblasts but do not form reproducible plaques on continuous cell culture lines. we tested medium overlay modifications for plaque formation of typhus group rickettsiae on the continuous fibroblast cell line vero76. a procedure involving primary overlay with medium at ph 6.8, which was followed 2 to 3 days later with secondary overlay ... | 1996 | 8818887 |
| [the ecology of rickettsia prowazekii]. | 1996 | 8820689 | |
| a chimeric disposition of the elongation factor genes in rickettsia prowazekii. | an exceptional disposition of the elongation factor genes is observed in rickettsia prowazekii, in which there is only one tuf gene, which is distant from the lone fus gene. in contrast, the closely related bacterium agrobacterium tumefaciens has the normal bacterial arrangement of two tuf genes, of which one is tightly linked to the fus gene. analysis of the flanking sequences of the single tuf gene in r. prowazekii shows that it is preceded by two of the four trna genes located in the 5' regio ... | 1996 | 8892818 |
| purification and assay of escherichia coli transcript cleavage factors grea and greb. | 1996 | 8902815 | |
| [probable nature of rickettsia prowazekii virulence]. | a 29.5 kda outer membrane protein (ompb) of r. prowazekii virulent breinl strain is known to differ from its counterpart in attenuated madrid e strain, while ompb of this latter one and of its virulent variant evir coincide in mobility. the infectivity of these strains for macrophages was previously shown to be different as well, and to correlate with their virulence. previously cloned r. prowazekii breinl strain, 1.644-bp insert from lambda gtll recombinant expressing as ompb in inducer-indepen ... | 1996 | 8927063 |
| rickettsia prowazekii sigma factor sigma 73 can be overexpressed in escherichia coli and promotes rna polymerase binding and transcription. | the sigma factor sigma 73 of the obligate intracytoplasmic bacterium rickettsia prowazekii was overexpressed and purified from escherichia coli. the rickettsial rpod gene encoding sigma 73 was cloned into a ndel-bamhi-cleaved pet-15b vector under control of t7 transcription and translation signals. the recombinant plasmid encoded a 75 kda fusion protein that was overproduced in e. coli bl21(de3) and purified from inclusion bodies after solubilization with guanidine hydrochloride and using his. b ... | 1996 | 8936316 |
| [transplacental transmission of the causative agent in experimental rickettsial infection]. | it was shown in the experiments on the model of typhus infection in guinea-pigs that the pathogenic organism can be inherited by foetus from the mother's organism in the period of acute infection. it is confirmed by the presence of positive seroconversion in 86% of born offsprings and the character of the immunity response after the control infection that is the initial formation of immunoglobulins of form g (ig g). at the same time there was no such a transfer of pathogenic organisms in 14% of ... | 1996 | 8991597 |
| [the effect of body immunological reactivity on the persistence of rickettsiae]. | correlation between the rate of seeding of organs by rickettsiae and duration of the exciter persistence and condition of immunological reactivity was established using the experimental rickettsial infection as a model. when using the preparation which stimulates the immunity indices, we have revealed that the number of rickettsiae in organs was less and the release from them was faster than under immunodepression. | 1996 | 9044714 |
| [lipid oxidation in bilayer lipid membranes linked with the reaction of oxidation of nad.h by atmospheric oxygen]. | it is shown that along with nad.h oxidation with air oxygen peroxide oxidation of lipids forming the membrane takes place in bilayer lipid membranes modified with ubiquinone. during nicotin amide oxidation proton absorption takes place. peroxide oxidation of lipids results in the liberation of h+ ions, which in its turn brings about the formation of protone-deficient or enriched (against aqueous solution) layers adjacent to the membrane. the potential value on the membrane is shown to depend on ... | 1996 | 178383 |
| the use of bronchoscopic sponge biopsy in the diagnosis of pulmonary neoplasms. | a modified method of transbronchoscopic sponge biopsy is presented a series of 212 patients is discussed. a diagnostic accuracy rate of 89 per cent was obtained in the 159 cases of proved carcinoma. false-positive and false-negative results are discussed. this technique was found to be an easy, rapid, accurate, and inexpensive method of obtaining samples for cytologic evaluation in the preoperative work-up of patients with suspected pulmonary malignancies. | 1996 | 173934 |
| [conception of programmed and standardized after-care of mammary carcinoma (author's transl)]. | optimal after-care of mammary carcinoma begins with the end of the primary therapy and lasts practically for a lifetime. the after-care consists of several inpatient follow-ups in specially equipped follow-up clinics, of outpatient after-care by the general practitioner and of care by appointment at the treatment center. standardized diagnostic methods combined with programmed appointments form the basis of an effective after-care for mammary carcinoma. the essence of a programmed and standardiz ... | 1996 | 177862 |
| squalene biosynthesis. role of the 3-methyl group in farnesyl pyrophosphate. | 1995 | 177475 | |
| a note on convolution and stable distributions in the nervous system. | 1995 | 173418 | |
| interaction of rickettsia prowazekii strains of different virulence with white rat macrophages. | the growth of mildly pathogenic strain e, its virulent revertant evir, and prototype virulent strain breinl of rickettsia prowazekii in peritoneal macrophage cultures of outbread white rats (wr) was evaluated by light microscopy and bioassay in chick embryos (ce). macrophage cultures infected with strain e were characteristic by limited number of infected cells, poor or moderate accumulation of rickettsiae in individual cells, poor or nil spread of infectious process during first 7 days of infec ... | 1995 | 8825301 |
| genomic evolution drives the evolution of the translation system. | our thesis is that the characteristics of the translational machinery and its organization are selected in part by evolutionary pressure on genomic traits have nothing to do with translation per se. these genomic traits include size, composition, and architecture. to illustrate this point, we draw parallels between the structure of different genomes that have adapted to intracellular niches independently of each other. our starting point is the general observation that the evolutionary history o ... | 1995 | 8721994 |
| [the epidemiological significance of human lice in relation to the characteristics of their parasitism]. | epidemiological significance of clothes and head louse is determined first of all by the type of "parasite-host" connection. clothes louse has an immediate contact with man only during bloodsucking, it dwells in clothes, consumes big portions of blood and has great productivity. temperature factor is very important for pediculus corporis. if the man's temperature is growing the louse will abandon him. the comparison of various literature data and proper author's information has shown that pedicu ... | 1995 | 8533365 |
| between bacteriology and virology: the development of typhus vaccines between the first and second world wars. | this paper provides an overview of the development of typhus vaccines between the first and second world wars. it is shown that there was a shift in the classification of the causal rickettsiae from being classed as bacteria to being conceptualised as a type of virus. this 'paradigm switch' stimulated interest in the possibility of producing an effective medicine. | 1995 | 8552752 |
| crystal structure of the grea transcript cleavage factor from escherichia coli. | transcription elongation factors stimulate the activity of dna-dependent rna polymerases by increasing the overall elongation rate and the completion of rna chains. one group of such factors, which includes escherichia coli grea, greb and eukaryotic sii (tfiis), acts by inducing hydrolytic cleavage of the transcript within the rna polymerase, followed by release of the 3'-terminal fragment. here we report the crystal structure of grea at 2.2 a resolution. the structure contains an amino-terminal ... | 1995 | 7854424 |
| the citrate synthase-encoding gene of rickettsia prowazekii is controlled by two promoters. | the transcripts of the citrate synthase-encoding gene (glta) in rickettsia prowazekii (rp), an obligate intracellular parasitic bacterium, were analyzed by rnase protection (rp), primer extension (pe) and in vitro transcription assays. analysis of the 5' end of the glta mrna by rp and pe assays revealed that there were two glta mrnas with the 5' ends located at 16 bp and 307 bp upstream from the glta coding region. since these two mrnas might represent two species of mrna transcribed from two di ... | 1995 | 7557459 |
| molecular characterization of an arabidopsis thaliana cdna encoding a novel putative adenylate translocator of higher plants. | we have isolated an arabidopsis thaliana cdna encoding a highly hydrophobic membrane protein of 589 amino acids which contains 12 potential transmembrane helices and shows a high degree of similarity (43.5% identity, 66.2% similarity) to the atp/adp translocase of the gram-negative bacterium rickettsia prowazekii, an obligate intracellular parasite responsible for the epidemic typhus. this rickettsial translocator resides in the cytoplasmic membrane and allows the bacterium to exploit the host c ... | 1995 | 7589569 |
| instability of rickettsia prowazekii rna polymerase-promoter complexes. | the rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute rna polymerase holoenzyme species. r. prowazekii rna polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding escherichia coli complexes were not. the r. prowazekii core played the major role in determining heparin sensitivity. | 1995 | 7592400 |
| evaluation of the pathogenic potential of rickettsia canada and rickettsia prowazekii organisms in dogs. | an unusual pattern of seroreactivity to antigens of rickettsial organisms (rickettsia rickettsii, r rhipicephali, r montana, and r bellii), particularly to r bellii antigen, was detected in 3 dogs during a 2-month period. thus, studies were initiated to clarify the pathogenic potential of the more distantly related rickettsial organisms (r canada and r prowazekii) in dogs. because r bellii are nonpathogenic rickettsiae that share numerous common properties with spotted fever-group and typhus-gro ... | 1995 | 7601694 |
| unusual organization of the rrna genes in rickettsia prowazekii. | we describe here the organization of the rrna genes in rickettsia prowazekii. in this organism, the 23s and the 5s rrna genes are tightly linked to each other, whereas the 16s rrna gene is separated from this cluster. the 23s-5s unit is preceded by the methionyl-trnafmet formyltransferase gene. | 1995 | 7608097 |
| ph and calcium dependence of hemolysis due to rickettsia prowazekii: comparison with phospholipase activity. | rickettsia prowazekii invades nucleated cells through phagocytosis and subsequently proliferates in the cytoplasm of the host cell. hemolysis and a phospholipase a2 (pla2) activity at neutral phs have previously been reported; even though the phagosomal environment is most likely acidic. we here show that r. prowazekii and r. typhi also lyse erythrocytes at mildly acidic phs, compatible with an early phagosomal compartment. for r. prowazekii, hemolysis at an acidic ph but not a neutral ph is enh ... | 1995 | 7622232 |
| intrinsic transcript cleavage activity of rna polymerase. | the grea and greb transcript cleavage factors of escherichia coli suppress elongation arrest and may have a proofreading role in transcription. with the use of e. coli grea-greb- mutant, rna polymerase is demonstrated to possess substantial intrinsic transcript cleavage activity. mildly alkaline ph mimics the effect of the gre proteins by inducing transcript cleavage in ternary complexes and antagonizing elongation arrest through a cleavage-and-restart reaction. thus, transcript cleavage constit ... | 1995 | 7538676 |
| rickettsia prowazekii, ribosomes and slow growth. | some bacteria, such as rickettsia prowazekii, grow slowly, not with anticipation of a future feast, but because it is evolutionarily advantageous to do so. this creates apparent paradoxes for understanding their physiology and biochemistry. these rickettsiae have a ribosome concentration higher than expected if these ribosomes support translation at rates comparable to those in escherichia coli. | 1995 | 7542988 |
| differentiation of bartonella-like isolates at the species level by pcr-restriction fragment length polymorphism in the citrate synthase gene. | the citrate synthase gene (glta) of bartonella henselae was cloned and sequenced to compare genetic divergence among alpha and gamma branches of the class proteobacteria and to develop enhanced genotypic reagents for b. henselae identification. b. henselae glta is 1,293 nucleotides in length and 63 to 66% homologous with corresponding gene sequences of rickettsia prowazekii, escherichia coli, and coxiella burnetii. the observed genetic variability suggests that glta sequences can provide a usefu ... | 1995 | 7545181 |
| genotype characterization of the bacterium expressing the male-killing trait in the ladybird beetle adalia bipunctata with specific rickettsial molecular tools. | the male-killing ladybird beetle (lb) bacterium (ab bacterium) was analyzed with specific rickettsial molecular biology tools in the lb adalia bipunctata strains. eight phenotype-positive lb strains showing mortality of male embryos were amplified with rickettsial genus-specific primers from the gene for citrate synthase (cs) and the gene for a 17-kda protein and spotted fever group-specific primers from the gene for the 120-kda outer membrane protein (ompb). the specificity of amplification was ... | 1995 | 7747963 |
| serological studies of the antigenic similarity between typhus group rickettsiae and weil-felix test antigens. | the sera from two patients with murine typhus reacted with whole cells of rickettsia prowazekii, r. typhi, and proteus vulgaris ox19, and with lipopolysaccharides (lps) from the spotted fever group rickettsia strain tt-118 and p. vulgaris ox19 in the enzyme-linked immunosorbent assay. sera from these patients reacted with ladder-like bands of lps from r. prowazekii and r. typhi in the immunoblot, whereas the reactivity of these sera with lps from p. vulgaris ox19 differed from each other. these ... | 1995 | 7783679 |
| genomic organization of the klebsiella pneumoniae cps region responsible for serotype k2 capsular polysaccharide synthesis in the virulent strain chedid. | the genomic organization of the chromosomal cps region that is responsible for capsular polysaccharide synthesis in klebsiella pneumoniae chedid (o1:k2) was investigated. deletion analyses and southern hybridization studies suggested that the central region of the cloned 29-kb bamhi fragment is indispensable for k2 capsular polysaccharide synthesis. the 24,329-bp nucleotide sequence of the klebsiella cps region was determined and deposited in the embl and genbank databases through ddbj and assig ... | 1995 | 7896702 |
| prevalence of antibodies to rickettsiae in the north-western part of bosnia and herzegovina. | the prevalence of rickettsial antibodies in north-western part of bosnia and herzegovina was studied. among 231 sera tested by complement fixation (cf) positive were: 61.5% for rickettsia typhi, 4.3% for r. prowazekii, 1.7% for r. conorii and 19.0% for coxiella burnetii. of 183 sera tested by indirect immunofluorescence assay (ifa) 37.7% reacted with r. typhi, 1.6% with r. conorii and 22.4% with c. burnetii. the results show that at least r. typhi and c. burnetii are highly endemic in this area. | 1995 | 8861855 |
| [the current problems in improving the vaccinal prophylaxis of typhus]. | 1995 | 9381858 | |
| studies of rickettsia prowazekii antigens in immunoblotting with specific sera of infected white mice. | five strains of rickettsia prowazekii different in origin, biological and genetic properties were compared in protein and lps patterns by the polyacrylamide gel electrophoresis and in antigenic properties by immunoblotting with specific sera of infected white mice. three virulent strains breinl, g and katsinjan had identical protein patterns and differed from isogenic pair of strains e and evir in the electrophoretic properties of 29-30 kda proteins. silver-strained lps patterns were different i ... | 1994 | 7739594 |
| crystallization of grea, a transcript cleavage factor from escherichia coli. | grea is a 17.6 kda protein from escherichia coli that induces cleavage of the nascent transcript in the elongating complex of rna polymerase, followed by release of the 3'-terminal fragment. crystals of grea have been obtained from polyethylene glycol 4000, 2-propanol and sodium citrate, ph 5.6 and have been propagated by a novel seeding procedure. the crystals diffract beyond 2 a resolution and belong to the orthorhombic space group p2(1)2(1)2(1), with cell dimensions a = 101.7 a, b = 42.22 a, ... | 1994 | 7932713 |
| genotypic characterization of rickettsiae by dna probes generated from rickettsia prowazekii dna. | southern blot analysis of hindiii-cleaved rickettsial dna was used for genotypic characterization of the typhus group (tg) species (r. prowazekii, r. typhi, r. canada) and a few species of the spotted fever group (sfg) rickettsiae (r. sibirica, r. conorii, r. akari). four different dna probes were employed. pbh11 and pbh13 probes were morphospecific hindiii fragments of r. prowazekii dna. mw218 probe contained the gene for 51 k antigen and mw264 probe contained the citrate synthase gene of r. pr ... | 1994 | 7976865 |
| ancestral divergence of rickettsia bellii from the spotted fever and typhus groups of rickettsia and antiquity of the genus rickettsia. | the eubacterial genus rickettsia belongs to the alpha subgroup of the phylum proteobacteria. this genus is usually divided into three biotypes on the basis of vector host and antigenic cross-reactivity characteristics. however, the species rickettsia bellii does not fit into this classification scheme; this organism has characteristics common to both the spotted fever group and the typhus group biotypes and also exhibits some unique features. sequences of the 16s rrna and 23s rrna genes from ric ... | 1994 | 7981106 |
| topology of the product binding site in rna polymerase revealed by transcript slippage at the phage lambda pl promoter. | in the presence of transcription substrates atp, ctp, and utp, a stable ternary complex containing tetranucleotide auca is formed on the phage lambda pl promoter (starting sequence c-3a-2c-1a+1u+2c+3a+4g+5). we show that in the absence of gtp or at undersaturating gtp concentrations the auca transcript synthesized at the +1 to +4 segment slips back by 3 nucleotides and is stabilized in the ternary complex in such a way that only its 2 3'-proximal bases remain paired to the -1/+1 positions of the ... | 1994 | 7989343 |
| the molar ratio of sigma 73 to core polymerase in the obligate intracellular bacterium, rickettsia prowazekii. | in the obligate intracellular parasitic bacterium, rickettsia prowazekii, the molar ratio of sigma 73 to core rna polymerase, that is, the degree of saturation of the core polymerase by the catalytically active sigma factor, was very low. this ratio was determined from the radioactivity in rickettsial rna polymerase immunoprecipitated from crude extracts of infected l929 cells in which the parasite was exponentially growing. if we assume that, as is true for the sigma subunit, in r. prowazekii a ... | 1994 | 8022263 |
| genomic identification of rickettsia slovaca among spotted fever group rickettsia isolates from dermacentor marginatus in armenia. | restriction fragment length polymorphism (rflp) analysis of polymerase chain reaction (pcr) amplified genes was used for genomic identification of armenian isolates of the spotted fever group (sfg) rickettsiae with unclear taxonomic position. analysis was performed by using one genus-specific primer pair derived from r. prowazekii citrate synthase gene and two species-specific primer pairs derived from r. rickettsii genes for 190 k and 120 k antigens following alui, psti and rsai digestion of am ... | 1994 | 7793355 |
| dermatologic manifestations of arthropod-borne diseases. | the arthropod-borne rickettsial, borrelial, and bacterial diseases of north america are a diverse group of disorders that produce a wide variety of cutaneous abnormalities. these dermatologic abnormalities are often valuable clinical clues that may reveal or suggest the correct diagnosis to the astute clinician. we review the usual and unusual dermatologic manifestations of rocky mountain spotted fever, murine and sylvatic typhus, rickettsialpox, ehrlichiosis, lyme disease, tick-borne relapsing ... | 1994 | 7814841 |
| sequence analysis of the rickettsia prowazekii gyra gene. | the rickettsia prowazekii (rp) gyra gene, which codes for a subunit of dna gyrase in this obligate intracellular bacterium, has been isolated and characterized. nucleotide sequence analysis revealed an open reading frame (orf), initiating with a gtg start codon, of 2718 bp that could encode a protein of 905 amino acids (aa) with a calculated m(r) of 101,048. the rp gyrase subunit a (gyra), when compared to gyra analogs of other bacterial species, exhibited 43 to 50% identity. alignment of the rp ... | 1994 | 7828873 |
| isolation of coxiella burnetii and of an unknown rickettsial organism from ixodes ricinus ticks collected in austria. | two strains of coxiella burnetii and two strains of an unidentified rickettsial organism were isolated for the first time from ixodes ricinus ticks collected in the alpine region of tirol, austria. the c. burnetii strains belong to the group of agents causing acute forms of q fever. the other two strains of isolated rickettsial agent share some antigenic epitopes with c. burnetii and r. prowazekii but they differ from them by their high sensitivity to freezing and refreezing and by poor multipli ... | 1994 | 7545588 |
| serological response of patients suffering from primary and recrudescent typhus: comparison of complement fixation reaction, weil-felix test, microimmunofluorescence, and immunoblotting. | microimmunofluorescence and western immunoblotting were compared with the classical complement fixation reaction and the weil-felix test to study the serological responses of patients to rickettsia prowazekii and both proteus vulgaris ox19 and ox2 during primary and recrudescent typhus infections. the serological response to r. prowazekii was found to be similar during primary and recrudescent typhus, and all sera examined contained antibodies to the same r. prowazekii cell structures. immunoglo ... | 1994 | 7496969 |
| rna cleavage and chain elongation by escherichia coli dna-dependent rna polymerase in a binary enzyme.rna complex. | in the absence of dna, escherichia coli rna polymerase (ec 2.7.7.6) can bind rna to form an equimolar binary complex with the concomitant release of the sigma factor. we show now that e. coli rna polymerase binds at a region near the 3' terminus of the rna and that an rna in such rna.rna polymerase complexes undergoes reactions previously thought to be unique to nascent rna in ternary complexes with dna. these include grea/greb-dependent cleavage of the rna and elongation by 3'-terminal addition ... | 1994 | 7513426 |
| [a method of fluorescent immunoenzyme analysis in the complex evaluation of viral and rickettsial vaccines]. | with the use of the unified indirect solid-phase fluorescent enzyme immunoassay the combined evaluation of the antigenic and immunogenic properties of experimental whole-virion inactivated virus vaccines against venezuelan and eastern equine encephalomyelitides, as well as of dried chemical typhus vaccine, has been made; their safety was determined indirectly by the content of ovalbumin. the protective role of antibodies evaluated by the solid-phase fluorescent enzyme immunoassay in typhus immun ... | 1994 | 7514329 |
| relationship of tumor necrosis factor alpha, the nitric oxide synthase pathway, and lipopolysaccharide to the killing of gamma interferon-treated macrophage-like raw264.7 cells by rickettsia prowazekii. | macrophage-like raw264.7 cells are killed by the combination of gamma interferon (ifn-gamma) treatment and infection with rickettsia prowazekii. the roles of tumor necrosis factor alpha (tnf-alpha), the nitric oxide synthase pathway, and lipopolysaccharide (lps) in this killing were investigated. r. prowazekii, both the breinl and madrid e strains, induced raw264.7 cells to produce tnf-alpha. however, dead rickettsiae (which cannot kill the ifn-gamma-treated raw264.7 cells) induced the productio ... | 1994 | 7514579 |
| cytokine sensitivity and methylation of lysine in rickettsia prowazekii evir and interferon-resistant r. prowazekii strains. | modified rickettsia prowazekii strains have been derived from the avirulent madrid e strain by passage in the lungs of white mice (strain evir) or by selection for resistance to gamma interferon (ifn-gamma) (strains 427-19 and 87-17) or alpha/beta interferon (ifn-alpha/beta) (strains 83-2p, 60p, 103-2p, and 110-1p). compared with the madrid e strain, strain evir has increased virulence (n. m. balayeva and v. n. nikolskaya, j. hyg. epidemiol. microbiol. immunol. 17:11-20, 1973) and a different ly ... | 1994 | 7518807 |
| the concentrations of stable rna and ribosomes in rickettsia prowazekii. | the obligate intracellular parasite, rickettsia prowazekii, is a slow-growing bacterium with a doubling time of about 10 h. in the present study, dna and rna were obtained from the rickettsiae by two independent methods, i.e. simultaneous isolation of dna and rna from the same sample by phenol:chloroform extraction and csci gradient centrifugation. in addition, ribosomal rna was obtained by sedimentation of partially purified ribosomes from the rickettsiae. the results demonstrated that, after c ... | 1994 | 7520114 |
| [the comparative characteristics of the immunogenic and immunomodulating activity of different antigenic preparations of the causative agent of q fever coxiella burnetii]. | the immunogenicity of the coxiella burnetii corpuscle antigen and two its components, obtained after the treatment with chloroform-methanol or extraction by trichloroacetic acid, was investigated. all the studied antigens were highly immunogenic. a strong correlation was discovered between the immunogenic and immunomodulating properties of the c. burnetii antigens. cells of the mononuclear phagocytic system are the targets for stimulating action of the studied antigens. a cyclic increase of anti ... | 1994 | 7535962 |
| [the immunogenic properties of liposomal preparations formed from a base of phospholipid antigenic complexes]. | 1994 | 7879550 | |
| analysis of hydrolytic products from choline-labeled host cell phospholipids during growth of rickettsia prowazekii. | a phospholipase activity has been associated with the interaction of rickettsia prowazekii with the surface of erythrocytes and competent host cells as well as during the growth of the rickettsiae within their host cells. both fatty acid and lysophosphatides have been found in the interaction of rickettsiae with the surface of eucaryotic cells; this finding provided strong evidence for the activity of a phospholipase a. however, fatty acids, but not lysophosphatides, were found during the growth ... | 1994 | 8132353 |
| isolation and characterization of the rickettsia prowazekii reca gene. | the reca gene has been isolated from rickettsia prowazekii, an obligate intracellular bacterium. comparison of the amino acid sequence of r. prowazekii reca with that of escherichia coli reca revealed that 62% of the residues were identical. the highest identity was found with reca of legionella pneumophila, in which 69% of the residues were identical. amino acid residues of e. coli reca associated with functional activities are conserved in rickettsial reca, and the r. prowazekii reca gene comp ... | 1994 | 8132476 |
| grea-induced transcript cleavage in transcription complexes containing escherichia coli rna polymerase is controlled by multiple factors, including nascent transcript location and structure. | the escherichia coli grea and greb proteins induce cleavage of 3' fragments from nascent transcripts in halted transcription complexes. we have overproduced and purified the grea protein and tested how it affects initiation, pausing, and termination by e. coli rna polymerase. recombinant grea induced cleavage of two to three nucleotide fragments in two promoter-proximal complexes, whereas an apparently endogenous cleavage removed a single larger fragment. both types of cleavage stopped once the ... | 1994 | 8071355 |
| grea-induced transcript cleavage is accompanied by reverse translocation to a different transcription complex conformation. | grea- and greb-induced transcript cleavage drives reverse translocation of escherichia coli rna polymerase on a dna template in the absence of ntps (feng, g.-h., lee, d. n., wang, d., chan, c. l., and landick, r. (1994) j. biol. chem. 269, 22282-22294, accompanying report). during transcript elongation, the sizes of the dna footprint and the single-stranded transcription bubble vary markedly among transcription complexes halted at different template positions. to test whether transcription compl ... | 1994 | 8071356 |
| [combined (associated) immunization against typhoid, typhus and plague]. | the article substantiates epidemiological expediency of complex (associated) immunization of servicemen and population against typhoid, typhus and plague in polyetiological zones of these infections, and also in cases of simultaneous proliferation of these diseases. for simultaneous preventive vaccination against these infections a complex immunization scheme was experimentally substantiated and clinically approved. it is based on national commercial vaccines and ensures a simultaneous administr ... | 1994 | 8091696 |
| analysis of the peptidoglycan of rickettsia prowazekii. | in the present study, peptidoglycan from rickettsia prowazekii, an obligate intracellular bacterium, was purified. the rickettsial peptidoglycan is like that of gram-negative bacteria; that is, it is sodium dodecyl sulfate insoluble, lysozyme sensitive, and composed of glutamic acid, alanine, and diaminopimelic acid in a molar ratio of 1.0:2.3:1.0. the small amount of lysine found in the peptidoglycan preparation suggests that a peptidoglycan-linked lipoprotein(s) may be present in the rickettsi ... | 1994 | 8300546 |
| [the protective activity of individual proteins and immunogenic fractions isolated from rickettsia prowazekii]. | the immunogenic properties and protective activity of basic protein i and tris-soluble antigens isolated from r. prowazekii were analyzed in comparison with those of chemical typhus vaccine on the model of anti-infectious immunity in guinea pigs. the analysis revealed that purified protein i has protective activity for guinea pigs, which is less pronounced in protein i from strain e with weak pathogenicity than protein i from strains evir and breinl. the activity and immunogenic properties of tr ... | 1994 | 8184624 |
| molecular packing of high density lipoproteins: a postulated functional role. | 1994 | 186302 | |
| current concepts in the management of small cell carcinoma of the lung. | small cell carcinoma of the lung should be viewed as a widely disseminated disease in all patients. therefore, systemic treatment with intensive combination chemotherapy should now be considered as the primary and initial treatment modality. the role of radiation therapy for local or involved-field needs to be re-examined to determine if such localized therapy following intensive combination chemotherapy offers any benefits in response rate or survival duration. the role of immunotherapy is not ... | 1994 | 205131 |
| extracondylar osteochondromas of the jaws. | a review of the literature and the addition of two new cases have revealed that according to a histologic criterion there are presently twenty reported cases of osteochondroma of the jaws located outside the condyles. although most of the extracondylar lesions have occurred on the coronoid process, cases are now reported on the maxilla and the body of the mandible. the clinical features of these lesions are compared with those located in the condyle, and the radiographic and surgical approaches ... | 1993 | 280859 |
| apical pulmonary disease in ankylosing spondylitis. | 1993 | 280798 | |
| factors associated with planned and unplanned nuptial births. | the incidence of unplanned pregnancy in a cohort of 1038 mothers of nuptial children was examined. the major findings were: (1) about one third of the pregnancies were unplanned but there was little evidence that unplanned children were unwanted; (2) there was no evidence to suggest that unplanned birth was related to poor education, low socio-economic status or lack of knowledge of contraception; (3) 40 percent of unplanned births were the result of contraceptive failure; (4) a common denominat ... | 1993 | 280785 |
| [a case report of adrenocorticotrophic hormone (acth) deficiency associated with the syndrome of inappropriate secretion of antidiuretic hormone (author's transl)]. | 1993 | 206636 |