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characterization of genes involved in long-term bacteremia in mice by bartonella birtlesii. 200516481533
bartonella henselae in porpoise blood.we report detection of bartonella henselae dna in blood samples from 2 harbor porpoises (phocoena phocoena). by using real-time polymerase chain reaction, we directly amplified bartonella species dna from blood of a harbor porpoise stranded along the northern north carolina coast and from a pre-enrichment blood culture from a second harbor porpoise. the second porpoise was captured out of habitat (in a low-salinity canal along the northern north carolina coast) and relocated back into the ocean. ...200516485476
bartonella quintana in cynomolgus monkey (macaca fascicularis).we identified a bartonella quintana strain by polymerase chain reaction amplification, cloning, and sequencing of dna extracted from lysed erythrocytes and cultured colonies grown from peripheral blood collected from a captive-bred cynomolgus monkey (macaca fascicularis). this report describes naturally acquired b. quintana infection in a nonhuman primate.200516485482
molecular characterization of haemoparasites infecting bats (microchiroptera) in cornwall, uk.the presence of haemoparasites from the order piroplasmida and the genera bartonella and trypanosoma was assessed in the blood of 60 bats, belonging to 7 species, inhabiting sites across cornwall in southwest england. dna extracted from macerated heart tissue was incorporated into taxon-specific polymerase chain reactions (pcrs) and amplification products were sequenced as a means of identifying, or assigning an identity, to detected haemoparasites. a piroplasmida species was detected in 6 pipis ...200516174413
the transcriptional response of human endothelial cells to infection with bartonella henselae is dominated by genes controlling innate immune responses, cell cycle, and vascular remodelling.the bacterial pathogen bartonella henselae (bh) is responsible for a broad range of clinical manifestations, including the formation of vascular tumours as the result of pathogen-triggered vasoproliferation. in vitro, the interaction of bh with human umbilical vein endothelial cells (huvec) involves (i) cytoskeletal rearrangements in conjunction with bacterial internalization, (ii) nuclear factor kappab (nfkappab)-dependent proinflammatory activation, (iii) the inhibition of apoptosis, and (iv) ...200516113825
the expanded spectrum of bartonellosis in children.bartonella spp cause various clinical syndromes immunocompetent and immunocompromised hosts. domestic cats are the natural reservoir, and vectors of b henselae. b henselae infection usually occurs early in childhood, is generally asymptomatic, and in most cases revolves spontaneously. it may, however, produce a wide spectrum of clinical symptoms, the most frequent feature being cat-scratch disease. disseminated atypical b. henselae infection may follow cat-scratch disease alter a symptom-free pe ...200516102656
abstracts for the 4th international conference on bartonella as emerging pathogens, 26-28 august 2005, uppsala, sweden. 200516100842
epidemiology of cat-scratch disease hospitalizations among children in the united states.cat-scratch disease (csd), caused by infection with bartonella henselae, affects both children and adults but is principally a pediatric disease. typical csd is generally benign and self-limited and is characterized by regional lymphadenopathy with fever. infections can, however, be accompanied by focal or diffuse inflammatory responses (atypical csd) involving neurologic, organ (liver/spleen), lymphatic or skeletal systems.200516094224
[multifocal chorioretinitis, papillitis, and recurrent optic neuritis in cat-scratch disease].to evaluate the causative factor for multifocal chorioretinitis, papillitis, and recurrent optic neuritis. a 41-year-old patient presenting multifocal choroiditis, papillitis, and recurrent optic neuritis was evaluated with funduscopy, angiography (fa), optical coherence tomography (oct), visual evoked potentials (vep), and numerous blood laboratory tests. fa and oct showed multifocal pigment epithelial detachments. vep showed typical changes for optic neuritis and papillitis. indirect fluoresce ...200516395190
[abrupt visual loss in children. three case studies of ocular bartonellosis].to evaluate the severity and progression of neuroretinitis in children caused by bartonella.200516395224
a nested-pcr with an internal amplification control for the detection and differentiation of bartonella henselae and b. clarridgeiae: an examination of cats in trinidad.bartonella species are bacterial blood parasites of animals capable of causing disease in both animals and man. cat-scratch disease (csd) in humans is caused mainly by bartonella henselae and is acquired from the cat, which serves as a reservoir for the bacteria. a second species, b. clarridgeiae is also implicated in the disease. diagnosis of bartonellosis by culture requires a week or more of incubation on enriched media containing blood, and recovery is often complicated by faster growing con ...200516098227
bartonella species in rodents and shrews in the greater jakarta area.in february 2004, we captured 221 rodents and shrews in the greater jakarta area as part of a study to determine the prevalence of rodent-associated vector-borne infections. microscopic examination of blood smears revealed 6% (13/218) to be positive for bartonella spp. the corresponding dna samples, either from blood blots or frozen spleen pieces and from fleas collected on these animals, were tested for evidence of bartonella infection by pcr, targeting the portions: 378bp and 930bp of the citr ...200516610656
[diagnostics of human granulocytic anaplasmosis].diagnostics of human granulocytic anaplasmosis. human granulocytic anaplasmosis is one of recently emerging tick-borne diseases. the paper summarizes current opinions on diagnostics of this zoonosis, including the recent recommendations of escar (european society of clinical microbiology and infectious diseases study group on coxiella, anaplasma, rickettsia and bartonella).200516838619
[capreolus capreolus and ixodes ricinus as a reservoir of bartonella in north-western poland].capreolus capreolus and ixodes ricinus as a reservoir of bartonella in north-western poland. the purpose of the study was to assess the prevalence bartonella in capreolus capreolus from north-western poland forest. supplementary, ticks infesting roe deer were also screened in order to ascertain their role as vectors and reservoirs of bartonella. the samples of blood from 98 animals from north-western poland were pcr-screened. bartonella dna was detected by using primers complementary to the inte ...200516838623
bartonella adhesin a mediates a proangiogenic host cell response.bartonella henselae causes vasculoproliferative disorders in humans. we identified a nonfimbrial adhesin of b. henselae designated as bartonella adhesin a (bada). bada is a 340-kd outer membrane protein encoded by the 9.3-kb bada gene. it has a modular structure and contains domains homologous to the yersinia enterocolitica nonfimbrial adhesin (yersinia adhesin a). expression of bada was restored in a bada-deficient transposon mutant by complementation in trans. bada mediates the binding of b. h ...200415534369
[subcutaneous nodules with malignant presentation, but caused by infection].three patients, a woman aged 32, a boy aged 6.5 and a man aged 56 years, presented with a subcutaneous mass suggesting a malignancy: respectively a rubbery swelling, painful to the touch below the left scapula, a partly massive, partly soft swelling on the inside of the left upper leg, and a non-fluctuating mass near the right eighth rib, parasternally. additional diagnostic investigation revealed an infectious cause: respectively mycobacterium tuberculosis, bartonella henselae and salmonella ty ...200415455512
[sudden sight impairment revealing a cat-scratch disease: report of three cases].cat-scratch disease is a frequent but innocuous cause of chronic lymphadenopathy in children. numerous atypical forms have been described. we report three cases of acute sight impairment revealing a cat-scratch disease. background: a 13 year-old boy and two girls aged 10 and 13 suffered from a sudden bilateral sight impairment with papillary edema, with fever in two cases. neurological examinations, x-rays and lumbar puncture results were found normal. however, all patients were tested positive ...200415475278
doxycycline, gastrointestinal bleeding, and thrombotic microangiopathy. 200415495239
[cat scrach disease]. 200415498227
isolation of bartonella schoenbuchensis from lipoptena cervi, a blood-sucking arthropod causing deer ked dermatitis.bartonella schoenbuchensis, which commonly causes bacteremia in ruminants, was isolated from the deer ked lipoptena cervi and was shown to localize to the midgut of this blood-sucking arthropod, causing deer ked dermatitis in humans. the role of b. schoenbuchensis in the etiology of deer ked dermatitis should be further investigated.200415528732
proteomic analysis of the sarcosine-insoluble outer membrane fraction of the bacterial pathogen bartonella henselae.bartonella henselae is an emerging zoonotic pathogen causing a wide range of disease manifestations in humans. in this study, we report on the analysis of the sarcosine-insoluble outer membrane fraction of b. henselae atcc 49882 houston-1 by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1-d sds-page) and two-dimensional nonequilibrium ph gradient polyacrylamide gel electrophoresis (2-d nephge). protein species were identified by matrix-assisted laser desorption/ioniz ...200415378747
a review of bacterial pathogens in ctenocephalides felis in new zealand.the cat flea, ctenocephalides felis, is the recognised vector of bartonella henselae, b. clarridgeiae and rickettsia felis. although these gram-negative bacteria were only described in the last decade, they are already known to cause a variety of diseases in people, particularly children and the immunosuppressed. such diseases include cat-scratch disease, bacillary angiomatosis, endocarditis, bacteraemia, encephalopathy, neuroretinitis, osteomyelitis and peliosis hepatis. although most infection ...200415768135
[study on the prevalence of bartonella species in rodent hosts from different environmental areas in yunnan].to investigate bartonella infections in small mammalian reservoir hosts from different environments and types of climate in yunnan.200415769319
[solitary nontuberculous mycobacterial infection of a cervical lymph node caused by bartonella henselae (cat scratch disease)].cat scratch disease (csd) is a common cause of subacute regional lymphadenopathy. cats are the principal reservoir of bartonella henselae, the etiologic agent in most cases of csd. because the histopathological findings are typical but not specific, a wide range of other agents must be considered in differential diagnosis. we report on a patient with a solitary swelling of the neck which remained over months with initial features suggestive of unspecific lymphadenitis, but who, after careful his ...200415365888
the right answer. 200415337400
cat-scratch disease: historic, clinical, and pathologic perspectives.cat-scratch disease (csd) initially was described in 1931, but the etiologic agent (bartonella henselae) was not elucidated until decades later. this disease is the most common cause of chronic lymphadenopathy among children and adolescents, characteristically manifesting as subacute regional lymphadenitis with an associated inoculation site due to a cat scratch or bite, often accompanied by fever. the hallmark histologic lesion is granulomatous inflammation with a central stellate microabscess. ...200415298152
serological diagnosis of human babesiosis by igg enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay (elisa) for the detection of igg antibodies to babesia microti antigen was developed. b. microti antigens were harvested from experimentally infected hamster blood and used as a coating antigen. the sensitivity and specificity of the igg elisa relative to immunofluorescent antibody assay (ifa) testing was 95.5% and 94.1%, respectively. according to the receiver operating characteristic curve analysis, the area under the curve was 0.987. no cross-reactivity of ...200415696612
[prevalence of bartonella henselae in stray and domestic cats in different italian areas: evaluation of the potential risk of transmission of bartonella to humans].bartonella henselae is the major etiological agent of cat scratch disease in humans. cats act as the natural reservoir of b. henselae and can transmit the infection to humans by bite or scratch. the diffusion of b. henselae was evaluated by seroprevalence and bacteremic status in different stray cat populations located in nine areas of northern italy. a total of 1585 cats were tested by blood culture and 361 (23%) resulted bacteremic; 1416 out off 1585 cats were also tested for bartonella hensel ...200415305701
emerging or re-emerging bacterial zoonotic diseases: bartonellosis, leptospirosis, lyme borreliosis, plague.there are a whole series of emerging and re-emerging zoonotic diseases present in the northern hemisphere and the author describes four of them, namely, bartonellosis, leptospirosis, lyme borreliosis and plague. reasons for the emergence or re-emergence of such diseases are not clear, but factors such as human demographics, economic development and land use, international travel and commerce, and microbial adaptation, are thought to be involved. control of emerging and re-emerging diseases has b ...200415702720
prospective studies of bartonella of rodents. part i. demographic and temporal patterns in population dynamics.the temporal dynamics of bartonella infections in a rodent community were described by repeatedly capturing and sampling individual animals. among six rodent species, from which bartonellae were isolated, cotton rats (sigmodon hispidus) accounted for > 98% of the bacteremic animals. all cotton rats captured four or more times were bartonella-culture positive at least once. the lowest monthly prevalence of bartonella in cotton rats was in june (49%) and the highest was in october (95%). prevalenc ...200415671735
prospective studies of bartonella of rodents. part ii. diverse infections in a single rodent community.the genetic diversity of bartonella species within a small mammal community and in individual cotton rats (sigmodon hispidus) was examined by trapping, capturing, sampling, and releasing of marked animals over a 17-month interval. based on sequence analyses of the bartonella glta gene, amplicons separated into four genogroups (a, b, c, and pin) containing 11 variants. although the prevalence of bacteremia due to different genogroups/variants of bartonella was temporally variable, variants of gen ...200415671736
semi-nested pcr detection of bartonella henselae in ixodes persulcatus ticks from western siberia, russia.questing adult ixodes persulcatus ticks from western siberia, russia were tested for infections with bartonella spp. using seminested pcr assay with primers specific to the groel gene. the proportion of ticks infected with bartonella spp. was 44% in 2002 (n = 50) and 38% in 2003 (n = 50). nucleotide sequences of a portion of the pcr products corresponded to bartonella henselae species.200415671737
bartonella henselae in african lion, south africa. 200415672532
bartonella clarridgeiae and b. henselae in dogs, gabon. 200415672535
[bartonellosis: emerging infection].the spectrum of bartonella infections in humans shows a constant increase. the number of bartonella species responsible of zoonoses has increased from one to 7 during the past ten years. in addition numerous orphan clinical manifestations are now associated to bartonella henselae infections. animals and particularly domestic cat are the main reservoirs of bartonella. cats are healthy carriers of b. henselae and b. clarridgeiae, and can be bacteremic for months to years. cat-to-cat transmission o ...200415673067
culture-negative bartonella endocarditis in a patient with renal failure: the value of molecular methods in diagnosis.members of the genus bartonella are increasingly recognised as a cause of culture-negative endocarditis, particularly in those patients with underlying risk factors (e.g., homelessness and alcoholism (b. quintana) or valvulopathy and cat ownership (b. henselae). the aortic and mitral-valves are most commonly involved. here, a case is reported of culture-negative right-sided endocarditis, without any of the above risk factors, due to bartonella sp. in a 69-year-old man who presented with acute re ...200415649011
molecular detection of bartonella spp. in the dental pulp of stray cats buried for a year.bartonella henselae causes chronic bacteremia in cats. to test if b. henselae dna can be recovered from the dental pulp of cats buried a year previously, we used pcr with primers for a sequence of the conserved groel to test 104 teeth from 11 cats. seven of the cats were found positive; canine teeth were more frequently positive than molar teeth. where pcr sequences could be determined, they were identical to those of b. henselae marseille (four cats), b. henselae houston (one cat) or similar to ...200415652295
guidelines for the diagnosis of tick-borne bacterial diseases in europe.ticks are obligate haematophagous acarines that parasitise every class of vertebrate (including man) and have a worldwide distribution. an increasing awareness of tick-borne diseases among clinicians and scientific researchers has led to the recent description of a number of emerging tick-borne bacterial diseases. since the identification of borrelia burgdorferi as the agent of lyme disease in 1982, 11 tick-borne human bacterial pathogens have been described in europe. aetiological diagnosis of ...200415606643
[bartonella and bartonellosis--emerging and re-emerging infections. taxonomy, bacteriology, pathogenesis and genetics].the review updates knowledge on the taxonomy, bacteriology and genetics of bartonella as well as pathogenesis of bartonellosis. the role of bartonella in human pathology, formerly considered to be rather modest, causes now growing anxiety. in this connection bartonella are now believed to be the causative agents of so-called emerging and re-emerging diseases, i.e. diseases, formerly unknown to man, and diseases, believed to be eradicated and playing at present no important role in human patholog ...200415636157
molecular genetic methods for the diagnosis of fastidious microorganisms.technological innovations in the detection and identification of microorganisms using molecular techniques such as polymerase chain reaction (pcr) have ushered in a new era with respect to diagnostic microbiology. pcr using universal or specific primers followed by identification of amplified product, mainly by sequencing, has enabled the rapid identification of cultured or uncultured bacteria. thus, pcr may allow quick diagnosis of infections caused by fastidious pathogens for which culture cou ...200415638838
population genetic analysis of bartonella bacilliformis isolates from areas of peru where carrion's disease is endemic and epidemic.carrion's disease is caused by infection with the alpha-proteobacterium bartonella bacilliformis. distribution of the disease is considered coincident with the distribution of its known vector, the sand fly lutzomyia verrucarum. recent epidemics of b. bacilliformis infections associated with atypical symptomatology in nonendemic regions have raised questions regarding the historic and present distribution of this bacterium and the scope of disease that infection causes. phylogenetic relationship ...200415297516
frontal and stealth attack strategies in microbial pathogenesis.interactions between microbes and human hosts can range from a benign, even symbiotic collaboration to a competition that may turn fatal--resulting in death of the host, the microbe or both. despite advances that have been made over the past decades in understanding microbial pathogens, more people worldwide still die every year from infectious disease than from any other cause. this highlights the relevance of continuing to probe the mechanisms used by microorganisms to cause disease, and empha ...200415241423
bartonella bovis in cattle in africa. 200415627528
multiplex real-time pcr for detection of anaplasma phagocytophilum and borrelia burgdorferi.a multiplex real-time pcr assay was developed for the simultaneous detection of anaplasma phagocytophilum and borrelia burgdorferi. the assay was tested on various anaplasma, borrelia, erhlichia, and rickettsia species, as well as on bartonella henselae and escherichia coli, and the assay was found to be highly specific for a. phagocytophilum and the borrelia species tested (b. burgdorferi, b. parkeri, b. andersonii, and b. bissettii). the analytical sensitivity of the assay is comparable to tha ...200415243077
rapid detection and differentiation of bartonella spp. by a single-run real-time pcr.a real-time pcr for the identification and differentiation of bartonella spp. based on the detection of mutations in an internal region of the glta gene by thermal analysis was developed. the assay included a simultaneous detection of the amplicons by direct hybridization of lcred and fluorescein-labelled probes coupled with melting curve analysis by the use of fluorescence resonance energy transfer technology. the protocol allowed establishing genospecie identity in less than 1 h without a need ...200415294318
bartonella henselae infection presenting as a unilateral panuveitis simulating vogt-koyanagi-harada syndrome.to report an unusual ocular manifestation of cat scratch disease.200415629311
chronic canine ehrlichiosis (ehrlichia canis): a retrospective study of 19 natural cases.nineteen dogs from greece with chronic ehrlichiosis were studied. the dogs exhibited bicytopenia or pancytopenia, bone marrow hypoplasia, seroreactivity to ehrlichia canis (e. canis) antigens, and had no history of drug or radiation exposure. anorexia, depression, severe bleeding tendencies, hypoalbuminemia, and increased serum alanine aminotransferase activity were also hallmarks of the disease. all these animals eventually died, irrespective of the treatment applied. some dogs were also serolo ...200415131097
prevalence and diversity of bartonella in rodents of northern thailand: a comparison with bartonella in rodents from southern china.we report results of the first study to investigate the distribution and diversity of bartonella in rodents from thailand. whole blood from 195 rodents, representing six species, was tested for the presence of bartonella species using standard culture techniques. isolates were obtained from 17 (8.7%) of the samples, and 14 of those isolates represented distinct strains, based upon partial sequencing of the citrate synthase (glta) gene. phylogenetic analysis of the isolates and other bartonella s ...200415100459
clinical problem-solving. index of suspicion. 200415128900
seroprevalence of bartonella henselae and bartonella quintana infections in children from central and northern jordan.to investigate the prevalence of antibodies to bartonella henselae (b.henselae) and bartonella quintana (b.quintana) among children from central and northern jordan.200415573198
prevalence of bartonella infection in wild african lions (panthera leo) and cheetahs (acinonyx jubatus).bartonella species are emerging pathogens that have been isolated worldwide from humans and other mammals. our objective was to estimate the prevalence of bartonella infection in free-ranging african lions (panthera leo) and cheetahs (acinonyx jubatus). blood and/or serum samples were collected from a convenience sample of 113 lions and 74 cheetahs captured in africa between 1982 and 2002. whole blood samples available from 58 of the lions and 17 of the cheetahs were cultured for evidence of bar ...200415135511
the bartonella henselae sucb gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease.bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. a recombinant clone expressing an immunoreactive antigen of b. henselae was isolated by screening a genomic dna cosmid library by western blotting with sera pooled from patients positive for b. henselae igg antibodies by indirect immunofluorescence (ifa). the deduced amino acid sequence of the 43.7 kda encoded protein was found to be 76.3 % identic ...200415585501
bartonella henselae causing severe and protracted illness in an otherwise healthy person.bartonella henselae is an emerging pathogen capable of causing severe disease. we report a case of severe protracted illness in an otherwise healthy police dog handler. the patient recovered slowly after antibiotic treatment. this case report emphasizes the importance of considering b. henselae infections in patients with fever of unknown origin.200415198195
flea-borne bartonella grahamii and bartonella taylorii in bank voles.bartonella species are increasingly associated with a range of human and animal diseases. despite this, we have a poor understanding of the ecology and epidemiology of many species, especially those circulating in wild populations. previous studies have demonstrated that a diverse range of bartonella species are abundant in wild rodent populations; little is known regarding their modes of transmission, although both direct and indirect routes have been suggested. in this study, with bank voles ( ...200415200860
the influence of climate on the epidemiology of bartonellosis in ancash, peru.the aim of this study was to evaluate the association between bartonellosis and selected climatic factors during the time periods 1983-1988 and 1995-99, which included two events of the el niño phenomenon (1986-88, 1997-98), and to identify a reliable climate parameter to be used as an alert indicator for bartonellosis outbreaks in ancash. the study site was ancash and its province carhuaz, peru. time-series cross-correlation analysis was used to assess the association between bartonellosis and ...200414964812
role of hippoboscidae flies as potential vectors of bartonella spp. infecting wild and domestic ruminants.the putative role of biting flies in bartonella transmission among ruminants was investigated. amplification of the bartonella citrate synthase gene from 83 hippoboscidae was detected in 94% of 48 adult lipoptena cervi flies, 71% of 17 adult hippobosca equina flies, 100% of 20 adult melophagus ovinus flies, and 100% of 10 m. ovinus pupae. our findings suggest that hippoboscidae play a role in the transmission of bartonella among ruminants. the vertical transmission of bartonella in m. ovinus and ...200415466580
detection of bartonella quintana by direct immunofluorescence examination of blood smears of a patient with acute trench fever.we report a case of bartonella quintana acute symptomatic infection in a homeless man, presenting as a typical trench fever. b. quintana has been retrieved in erythrocytes in large clusters and in erythroblasts. direct immunofluorescence of blood smears allows a rapid diagnosis.200415472378
[carrión-disease--history of a long-forgotten malady]. 200414978887
acute hemiplegia associated with cat-scratch disease.cat scratch disease (csd) is an infectious illness caused by a gram-negative rod named bartonella henselae. typical csd is characterized by a small skin lesion at the site of a scratch or a bite, followed by regional lymphadenopathy, one to two weeks later. atypical forms may present as ocular manifestations, neurological manifestations, hepatosplenic involvement and vertebral osteomyelitis. among neurological complications, encephalopathy is by far the most common. other neurological manifestat ...200415476060
molecular and cellular basis of bartonella pathogenesis.the genus bartonella comprises several important human pathogens that cause a wide range of clinical manifestations: cat-scratch disease, trench fever, carrion's disease, bacteremia with fever, bacillary angiomatosis and peliosis, endocarditis, and neuroretinitis. common features of bartonellae include transmission by blood-sucking arthropods and the specific interaction with endothelial cells and erythrocytes of their mammalian hosts. for each bartonella species, the invasion and persistent int ...200415487942
molecular detection of bartonella henselae dna in the dental pulp of 800-year-old french cats.bartonella species are responsible for chronic bacteremia in domestic cats, which raises a question about the antiquity of the relationship between bartonella species and cats that act as reservoirs for the organism. the sequencing of bartonella pap31 and groel genes from the dental pulp of cats dating from the 13th to 16th centuries identified the presence of b. henselae genotype houston; the observation of a unique mutation in the results of pcr assays for bartonella species ruled out modern d ...200415494918
clinicopathological abnormalities and treatment response in 24 dogs seroreactive to bartonella vinsonii (berkhoffii) antigens.bartonella vinsonii (b. vinsonii) subspecies berkhoffii is a recently recognized cause of endocarditis, myocarditis, and granulomatous disease in dogs. in an effort to elucidate other potential disease manifestations, the case records of 24 dogs that were seroreactive to b. vinsonii (berkhoffii) antigens were studied retrospectively. diagnoses included immune-mediated hemolytic anemia, neutrophilic or granulomatous meningoencephalitis, neutrophilic polyarthritis, cutaneous vasculitis, and uveiti ...200415007043
[screening for antibodies against zoonotic agents among employees of the zoological garden of vienna, schönbrunn, austria].the aim of this study was to investigate the prevalence of antibodies against zoonotic agents in employees of the zoological garden of vienna, schönbrunn, austria. sixty out of 120 employees participated in the study. in 97% of them antibodies to at least one zoonotic agent were identified. only two participants were free of antibodies to the zoonotic agents tested. the following seroprevalences (in brackets) were obtained: viral zoonotic (and potentially zoonotic) agents: influenzavirus a/h1n1 ...200415495931
acinetobacter baumannii in human body louse.while we were isolating bartonella quintana from body lice, 40 acinetobacter baumannii strains were also isolated and genotyped. one clone was unique and the other was ampicillin susceptible. a. baumannii dna was later detected in 21% of 622 lice collected worldwide. these findings show an a. baumannii epidemic in human body lice.200415498175
identification of bartonella species in rodents, shrews and cats in denmark: detection of two b. henselae variants, one in cats and the other in the long-tailed field mouse.small mammals and stray cats were trapped in two areas of north zealand, denmark, and their blood cultured for hemotrophic bacteria. bacterial isolates were recovered in pure culture and subjected to 16s rdna gene sequencing. bartonella species were isolated from five mammalian species: b. grahamii from microtus agrestis (field vole) and apodemus flavicollis (yellow-necked field mouse); b. taylorii from m. agrestis, a. flavicollis and a. sylvaticus (long-tailed field mouse); b. tribocorum from a ...200415511270
latent infective endocarditis: epidemiology and clinical characteristics of patients with unsuspected endocarditis detected after elective valve replacement.the diagnosis of infective endocarditis is usually made on the basis of clinical and laboratory criteria and may be confirmed by histologic examination or culture of excised valves. we tried to determine the incidence and significance of inflammatory changes in valves excised during operations for reasons other than infective endocarditis.200415511445
epilepsia partialis continua in cat scratch disease.cat scratch disease (csd) is a world-wide, diffuse, non-epidemic infection caused by the gram-negative bacillus bartonella henselae. the occurrence of encephalopathy represents an infrequent and atypical complication, whose manifestations include ischemic strokes, transverse myelitis and epileptic seizures. status epilepticus has been described as the most frequent emergency in csd encephalopathy. in this report, we describe a case of csd complicated by an epilepsia partialis continua (epc) mani ...200415010059
development of iga nephritis following cat scratch disease in a 13-year-old boy.we describe a 13-year-old boy who presented with hematuria and intermittent low-grade proteinuria at the time when he was diagnosed with cat scratch disease. two months before presentation, he had a negative urinalysis during a routine physical evaluation. he continued to have microscopic hematuria for the next 6 months, when he developed gross hematuria and recurrence of low-grade proteinuria. the renal biopsy showed evidence of mild/moderate iga nephropathy. we speculate that the immunological ...200415015065
the virb type iv secretion system of bartonella henselae mediates invasion, proinflammatory activation and antiapoptotic protection of endothelial cells.bartonella henselae is an arthropod-borne zoonotic pathogen causing intraerythrocytic bacteraemia in the feline reservoir host and a broad range of clinical manifestations in incidentally infected humans. remarkably, b. henselae can specifically colonize the human vascular endothelium, resulting in inflammation and the formation of vasoproliferative lesions known as bacillary angiomatosis and bacillary peliosis. cultured human endothelial cells provide an in vitro system to study this intimate i ...200415049812
a child with bartonella henselae osteomyelitis of the right humerus.we present a case of a 22-month-old child with swollen upper part of the right arm and osteolytic lesion of the right humerus, which resembled a neoplastic process. epidemiological history revealed no scratch marks on the skin or cutaneous papule or pustule. presumptive diagnosis of hematogenous osteomyelitis was established, but treatment with fusidic acid was unsuccessful. histological examination of the bioptic specimen of the soft tissue swelling showed a lymph node morphology, with numerous ...200415075043
guidelines for the antibiotic treatment of endocarditis in adults: report of the working party of the british society for antimicrobial chemotherapy.the bsac guidelines on endocarditis were last published in 1998. the guidelines presented here have been updated and extended to reflect changes in both the antibiotic resistance characteristics of causative organisms and the availability of new antibiotics. randomized, controlled trials suitable for the development of evidenced-based guidelines in this area are still lacking, and therefore a consensus approach has again been adopted. the guidelines cover diagnosis and laboratory testing, suitab ...200415546974
seroprevalences of antibodies against bartonella henselae and toxoplasma gondii and fecal shedding of cryptosporidium spp, giardia spp, and toxocara cati in feral and pet domestic cats.to compare seroprevalences of antibodies against bartonella henselae and toxoplasma gondii and fecal shedding of cryptosporidium spp, giardia spp, and toxocara cati in feral and pet domestic cats.200415552314
in vitro model of bartonella henselae-induced angiogenesis.bartonella henselae is a gram-negative pathogen that causes angiogenesis. here, i establish in vitro models to study bartonella-induced blood vessel formation. i found that b. henselae induces long-term endothelial survival and tubular differentiation within type i collagen matrix.200415557658
molecular detection of bartonella quintana dna in the dental pulp of a homeless patient.dental pulp has been proposed as a suitable tissue sample for the identification of pathogenic organisms. using pcr with two specific gene targets, bartonella quintana dna was detected in the dental pulp extracted from the tooth of a homeless patient. the patient had been bacteremic 6 months previously but was not when the tooth was sampled.200415558347
bartonelosis (carrion's disease) in the pediatric population of peru: an overview and update.bartonellosis, or carrion's disease, is an endemic and reemerging disease in peru and ecuador. carrion's disease constitutes a health problem in peru because its epidemiology has been changing, and it is affecting new areas between the highland and the jungle. during the latest outbreaks, and previously in endemic areas, the pediatric population has been the most commonly affected. in the pediatric population, the acute phase symptoms are fever, anorexia, malaise, nausea and/or vomiting. the mai ...200415798808
neck abscess secondary to cat-scratch disease.a 7-year-old boy was referred to us for evaluation of an enlarging neck mass. the results of his primary care physician's initial clinical examination suggested lymphadenopathy secondary to lymphadenitis, and the patient was treated over a 4-week period with two rounds of antibiotics. however, the mass did not resolve, and it subsequently became fluctuant. the patient was referred to our institution, where we diagnosed cat-scratch disease.200415628637
antibodies reactive with bartonella henselae and ehrlichia canis in dogs from the communal lands of zimbabwe.the prevalences of antibodies against bartonella henselae and ehrlichia canis were determined in sera from 228 dogs in 5 communal lands of zimbabwe, areas where traditional subsistence agro-pastoralism is practised. the sera were collected from apparently healthy dogs during routine rabies vaccination programmes and tested with indirect fluorescent antibody assays using b. henselae (houston-i) and e. canis (oklahoma) as antigens. we found reactive antibodies (> or =1:80) against b. henselae in 1 ...200415628802
ddei rflp for 16s rdna typing in bartonella henselae. 200415585508
late onset of serologic positive titers in a patient with parinaud's oculoglandular syndrome.to report a case of parinaud's oculoglandular syndrome (pos) in which, despite the initially negative, a late onset of serologic positive titers was observed.200415590554
bacterial models for tumor development. mini-review.the tumor-inducing effects of agrobacterium, bartonella and helicobacter bacterial species are compared step by step. an analogy for the existence of these individual steps is considered in connection with the development of cancer. the transformations of eukaryotic cells occur in particular in the type iv secretion system, i.e. involving the simultaneous transmission of dna and protein from bacterial cells to eukaryotic cells. thus, transfected cells facilitate the indefinite growth of tissue c ...200415571072
[bartonelloses].bartonellae belong to less known causal agents of many human diseases. they are gram-negative bacteria growing slowly on culture media enriched with hemin or bovine serum. the genus bartonella, which currently involves more than 15 species, is present worldwide. bartonellae live in natural foci in dependence on the occurrence of natural host (rodents, felines, canidae, human) and insect vector (flea, tick, louse). by reservoir animals they usually cause permanent intraerythrocytic bacteraemia wi ...200415558448
a mechanic with a bad valve: blood-culture-negative endocarditis.a 33-year-old man with a known bicuspid aortic valve presented with fever, chills, progressive fatigue, anorexia, and night sweats. echocardiography confirmed aortic-valve endocarditis, but blood cultures remained negative. bartonella henselae endocarditis was ultimately confirmed by serology as well as by immunohistochemistry and pcr testing of the excised valve. the patient recovered with appropriate antibiotic therapy. b henselae is a common cause of culture-negative endocarditis. it predomin ...200415567127
diversity of bartonellae associated with small mammals inhabiting free state province, south africa.the prevalence and diversity of bartonellae infecting the blood of 10 small mammal species inhabiting nine nature reserves of the free state province, south africa, was assessed using phenotypic, genotypic and phylogenetic methods. of 86 small mammals sampled, 38 animals belonging to five different species yielded putative bartonellae. thirty-two isolates were confirmed as bartonellae and were characterized by comparison of partial citrate synthase gene (glta) sequences. phylogenetic reconstruct ...200415545418
bartonella quintana-induced apoptosis inhibition of human endothelial cells is associated with p38 and sapk/jnk modulation and with stimulation of mitosis.previous studies demonstrated that live bartonella quintana often induces angioproliferative lesions in humans. it modulates endothelial cell apoptotic and inflammatory patterns, thus inducing a very early overexpression of caspase 8 and apaf-1 and increasing mrna production of tnf-alpha, interleukin-8, and e-selectin. however, starting at 10 hours postinfection, the bacteria provoke antiapoptotic effects that induce an increase of bcl-2 gene transcription. to gain further insight into the cellu ...200415541600
organismal effects of pesticide exposure on meadow voles (microtus pennsylvanicus) living in golf course ecosystems: developmental instability, clinical hematology, body condition, and blood parasitology.this is the second of two articles reporting the results of a nonlethal biomonitoring study that quantified the effects of pesticide exposure on meadow voles (microtus pennsylvanicus) living in golf course ecosystems of the ottawa/gatineau region (on and pq, canada, respectively). in the present article, we describe results of measurements regarding developmental instability (e.g., fluctuating asymmetry), congenital birth defects (e.g., skeletal terata), clinical hematology (e.g., differential c ...200415376537
[newly emerging infections: catscratch disease]. 200415700401
high prevalence of antibodies to bartonella henselae among italian children without evidence of cat scratch disease.few data are available on the seroprevalence of antibodies to bartonella henselae among children. we retrospectively evaluated the presence of immunoglobulin g and m class antibodies to b. henselae in 508 children living in central italy who were apparently free of any features suggesting b. henselae infection. we found that b. henselae infection is common among children in central italy, occurs early in life, is in most cases asymptomatic, and resolves spontaneously.200414679462
factors affecting the component community structure of haemoparasites in common voles ( microtus arvalis) from the mazury lake district region of poland.the prevalence and abundance of infections with haemoparasites were studied over a 4-year period in microtus arvalis (common vole, n =321) sampled from fallow grassland sites in north-eastern poland. total species richness was five (prevalence= haemobartonella sp. 63.9%, bartonella spp. 27.7%, babesia microti 9.0%, trypanosoma sp. 8.4% and and hepatozoon lavieri 3.1%) with 76.9% of the voles carrying at least one species and a mean infracommunity species richness of 1.1. variation in species ric ...200414714180
bartonella chomelii sp. nov., isolated from french domestic cattle (bos taurus).two strains of bacteria isolated from the blood of french domestic cows were found to be similar to bartonella species on the basis of phenotypic characteristics. genotypic analysis based on sequence comparison of the 16s rrna and citrate synthase (glta) genes and on dna-dna hybridization showed that the two isolates represent a distinct and new species of bartonella. moreover, the phylogenetic analysis inferred from comparison of 16s rrna and glta sequences demonstrated that the new bartonella ...200414742483
hemophagocytic syndrome in renal transplant recipients: report of 17 cases and review of literature.hemophagocytic syndrome (hps) combines febrile hepatosplenomegaly, pancytopenia, hypofibrinemia, and liver dysfunction. it is defined by bone marrow and organ infiltration by activated, nonmalignant macrophages phagocytizing blood cells. hps is often caused by an infectious or neoplastic disease and has rarely been described in renal transplant recipients.200414742988
bartonella henselae infection associated with neuroretinitis, central retinal artery and vein occlusion, neovascular glaucoma, and severe vision loss.to report a case of bartonella henselae infection.200414700670
antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens.the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ...200414765731
structure and biological activity of the short-chain lipopolysaccharide from bartonella henselae atcc 49882t.the facultative intracellular pathogen bartonella henselae is responsible for a broad range of clinical manifestations, including the formation of vascular tumors as a result of increased proliferation and survival of colonized endothelial cells. this remarkable interaction with endotoxin-sensitive endothelial cells and the apparent lack of septic shock are considered to be due to a reduced endotoxic activity of the b. henselae lipopolysaccharide. here, we show that b. henselae atcc 49882(t) pro ...200414766898
growth characteristics of bartonella henselae in a novel liquid medium: primary isolation, growth-phase-dependent phage induction, and metabolic studies.bartonella henselae is a zoonotic pathogen that usually causes a self-limiting infection in immunocompetent individuals but often causes potentially life-threatening infections, such as bacillary angiomatosis, in immunocompromised patients. both diagnosis of infection and research into the molecular mechanisms of pathogenesis have been hindered by the absence of a suitable liquid growth medium. it has been difficult to isolate b. henselae directly from the blood of infected humans or animals or ...200414766538
first isolation of bartonella henselae type i from a cat-scratch disease patient in japan and its molecular analysis.we isolated bartonella henselae from an inguinal lymph node of a 36-year-old male patient with cat-scratch disease. the patient had many areas of erythema on his body, swelling of the left inguinal lymph nodes with pain and slight fever. the diagnosis was made on the basis of polymerase chain reaction for b. henselae dna from the lymph node biopsies and blood sample, and isolation of the organism, histology of the lymph node and serology with an indirect immunofluorescent antibody test. we also ...200414978335
natural breeding places of phlebotomine sandflies.methods of finding larvae and pupae of phlebotomine sandflies (diptera: psychodidae) are described and the known types of breeding sites used by sandflies are listed. three ways of detecting sandfly breeding places are the use of emergence traps placed over potential sources to catch newly emerged adult sandflies; flotation of larvae and pupae from soil, etc., and desiccation of media to drive out the larvae. even so, remarkably little information is available on the ecology of the developmental ...200415009450
prevalence of bartonella clarridgeiae and bartonella henselae in domestic cats from france and detection of the organisms in erythrocytes by immunofluorescence.the prevalence of bartonella infection in a pet cat population from france was found to be 8.1% (8 of 99 cats). the intraerythrocytic location of bartonella clarridgeiae is shown for the first time, and we show that immunofluorescence detection of the organism in erythrocytes correlates with the number of bacteria in blood.200415013998
[nanobacteria--microbiological characteristics].we have reviewed recent publications regarding the microbiological characteristic and pathogenicity of a novel infectious agent, the mineral-forming, sterile-filterable, slow-growing gram-negative nanobacteria, detected in bovine/human blood, kidney cyst fluid, urine and kidney stones. according to their 16s rdna structure, nanobacteria belong to the alpha-2 proteobacteria, subgroup, which includes the brucella and bartonella species. their cell diameter is 0.2-0.5 microm (the smallest known cel ...200415069381
study of genotypes and virb4 secretion gene of bartonella henselae strains from patients with clinically defined cat scratch disease.bartonella henselae is the causative agent of cat scratch disease (csd), which usually presents as a self-limiting lymphadenopathy. occasionally, the bacteria will spread and be responsible for tissue and visceral involvement. two b. henselae genotypes (genotypes i and ii) have been described to be responsible for uncomplicated csd on the basis of 16s rrna sequence analysis. a type iv secretion system (t4ss) similar to the virulence-associated virb system of agrobacterium tumefaciens was recentl ...200415070983
intraocular detection of bartonella henselae in a patient with hla-b27 uveitis.bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying hla-b27 uveitis on the basis of immunodetection and molecular detection of the organism, a marseilles genogroup and a cal-1 genotype strain, in the vitreous fluid. this case illustrates that b. henselae should be included in the differential diagnosis of uveitis and the usefulness of immunodetection for rapid and specific diagnosis.200415071060
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