Publications

detection of an ambler class d oxa-48-type β-lactamase in a klebsiella pneumoniae strain in the netherlands.	traditionally, bacteria in the netherlands have low levels of resistance to antibiotics. this report describes what is believed to be the first carbapenem-resistant klebsiella pneumoniae producing an oxa-48 type β-lactamase in the netherlands. the isolate co-produced a ctx-m-15 type β-lactamase and was recovered from a patient who was transferred from a hospital in india to an intensive care unit in the netherlands. his recovery in the netherlands was complicated by pneumonia due to the carbapen ...	2011	21252273
plasmid-borne 16s rrna methylase arma in aminoglycoside-resistant klebsiella pneumoniae in poland.	we characterized 17 clinical isolates of klebsiella pneumoniae producing 16s rrna methylase arma. the isolates originated in poland from 2002 to may 2010 and encompassed four xbai-pfge clusters. all the isolates were resistant to amikacin, gentamicin and kanamycin (mic range: 256-1024 mg l(-1)) and carried the arma gene on a large plasmid of approximately 90 or 130 kb in 15 and 2 isolates, respectively. the arma gene was found in a ~10 kb clai restriction fragment of the large plasmid and was fl ...	2011	21252274
molecular characterization and epidemiology of extended-spectrum beta-lactamase-producing escherichia coli and klebsiella pneumoniae isolates in the united arab emirates.	the aim of this prospective study was to assess the extent of dissemination of extended-spectrum ß-lactamase (esbl)-producing enterobacteriaceae at the hospital level in the united arab emirates (uae).	2011	21252576
comment on 'pink hypopyon caused by klebsiella pneumonia'.		2011	21252947
identification of ctx-m15-, shv-28-producing klebsiella pneumoniae st15 as an epidemic clone in the copenhagen area using a semi-automated rep-pcr typing assay.	rapid molecular typing methods can be a valuable aid in the investigation of suspected outbreaks. we used a semi-automated repetitive sequence-based polymerase chain reaction (rep-pcr) typing assay and pulsed field gel electrophoresis (pfge) to investigate the relationship between local klebsiella pneumoniae (k. pneumoniae) producing extended spectrum ß-lactamases (esbls) and their relation to recognized danish outbreak strains. pfge and rep-pcr produced similar clustering among isolates. indivi ...	2011	21253799
site-directed alkylation studies with lacy provide evidence for the alternating access model of transport.	in total, 59 single cys-replacement mutants in helix vii and helix x of the lactose permease of escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of cys accessibility or reactivity. for both helices, accessibility/reactivity is relatively low at the level of the sugar-binding site where the helices are tightly packed. however, labeling of cys substitutions in helix vii with tetramethylrhodamine-5-maleimide decreases ...	2011	21254783
investigating the impact of the definition of previous antibiotic exposure related to isolation of extended spectrum β-lactamase-producing klebsiella pneumoniae.	previous antibiotic exposure is a risk factor for extended spectrum β-lactamase (esbl)-producing klebsiella pneumoniae isolation, but the optimal definition of previous antibiotic exposure remains unclear.	2011	21255875
multimerization-defective variants of dodecameric secretin puld.	the c-terminal core domain of the secretin puld from klebsiella oxytoca forms heat-resistant dodecameric complexes within less than 10min in an escherichia coli in vitro transcription-translation system containing liposomes, and is toxic when made in the cytoplasm without a signal peptide. random mutagenesis of dna encoding this region of puld revealed that amino acid changes throughout almost its entire length abolished toxicity. most of the amino acid substitutions engendered by the mutations ...	2011	21256212
sources of klebsiella and raoultella species on dairy farms: be careful where you walk.	klebsiella spp. are a common cause of mastitis, milk loss, and culling on dairy farms. control of klebsiella mastitis is largely based on prevention of exposure of the udder to the pathogen. to identify critical control points for mastitis prevention, potential klebsiella sources and transmission cycles in the farm environment were investigated, including oro-fecal transmission, transmission via the indoor environment, and transmission via the outdoor environment. a total of 305 samples was coll ...	2011	21257074
carbapenem resistance in klebsiella pneumoniae due to the new delhi metallo-β-lactamase.	carbapenem resistance in klebsiella pneumoniae is most notably due to the k. pneumoniae carbapenemase (kpc) β-lactamase. in this report, we describe the occurrence of a newly described mechanism of carbapenem resistance, the ndm-1 β-lactamase, in a patient who received medical attention (but was not hospitalized) in india.	2011	21258100
new delhi metallo-ß-lactamase and multidrug resistance: a global sos?		2011	21258101
risk factors for the acquisition of nosocomial infection with carbapenem-resistant klebsiella pneumoniae.	carbapenem-resistant klebsiella pneumoniae (crkp) has been increasingly reported all over the world. in this study, we aimed to investigate the risk factors for the acquisition of nosocomial crkp infections.	2011	21258230
successful control of peristomal infection by introducer-type percutaneous endoscopic gastrostomy: a retrospective historical control study.	the techniques of percutaneous endoscopic gastrostomy are classified as the pull and introducer methods. peristomal infection is the most common procedure-related complication in the pull method.	2011	21259072
endogenous endophthalmitis associated with liver abscess caused by klebsiella pneumoniae.	to report two unusual cases of endogenous endophthalmitis associated with liver abscess caused by klebsiella pneumoniae. retrospective, interventional case series. two patients, known to have type ii diabetes mellitus, presented with sudden visual loss following several days of abdominal pain. examinations and investigations revealed endogenous endophthalmitis caused by k. pneumoniae. despite treatment in the form of intravitreal injection of antibiotics in the first patient and pars plana vitre ...	2011	21264489
klebsiella pneumoniae esbl producers responsible for severe utis in a renal transplant unit.		2011	21264678
antimicrobial isopropenyl-dihydrofuranoisoflavones from crotalaria lachnophora.	two new isopropenyl-dihydrofuranoisoflavones exhibiting antimicrobial properties have been isolated along with eight known compounds from the cameroonian medicinal plant crotalaria lachnophora. the structures of the new compounds were elucidated by 1d and 2d nmr spectroscopy and high-resolution mass spectrometry as 7,2',4'-trihydroxy-5''-isopropenyl-4'',5''-dihydrofurano[2'',3'':5,6]isoflavone (1) and 4,8-dihydroxy-2-isopropenyl-2,3-dihydro-5h-[1]benzofuro[2,3-b]furo[3,2-g]chromen-5-one (2). the ...	2011	21265557
electron transfer and half-reactivity in nitrogenase.	nitrogenase is a globally important enzyme that catalyses the reduction of atmospheric dinitrogen into ammonia and is thus an important part of the nitrogen cycle. the nitrogenase enzyme is composed of a catalytic molybdenum-iron protein (mofe protein) and a protein containing an [fe4-s4] cluster (fe protein) that functions as a dedicated atp-dependent reductase. the current understanding of electron transfer between these two proteins is based on stopped-flow spectrophotometry, which has allowe ...	2011	21265773
is selective digestive decontamination useful in controlling aerobic gram-negative bacilli producing extended spectrum beta-lactamases?	to identify outbreak episodes of either carriage or infection due to extended spectrum beta-lactamases producing aerobic gram-negative bacilli (agnb-esbl); to establish whether agnb-esbl, sensitive to tobramycin, become resistant over time; and to evaluate the impact of selective decontamination of the digestive tract (sdd) on abnormal carriage of agnb-esbl.	2011	21268690
evaluation of the vitek 2 ast-n111 card for detection of extended-spectrum beta-lactamases (esbls) in escherichia coli, klebsiella pneumoniae, and klebsiella oxytoca compared to esbl etests and combination disk methods.	the vitek 2 ast-n111 card was evaluated for detection of extended-spectrum beta-lactamases (esbls) by testing 51 esbl positive and 50 esbl negative isolates of e. coli, k. pneumoniae, and k. oxytoca. the occurrence of beta-lactamase genes was confirmed by pcr and sequencing. the advanced expert system (aes) of the vitek 2 system achieved sensitivity and specificity values of 100% and 96.0%, respectively. the esbl test of the vitek 2 ast-n111 card showed a sensitivity of 92.1% and a specificity o ...	2011	21271268
a comparative study of induction of pneumonia in mice with planktonic and biofilm cells of klebsiella pneumoniae.	in the present study, the course of acute pneumonia in normal balb/c mice infected by intranasal inoculation of planktonic and preformed biofilm cells (3 days old) of klebsiella pneumoniae b5055 was studied and compared. with both cell forms the peak of infection was observed on the third post infection day, as assessed on the basis of lung bacterial load and corresponding pathology. there was an intense neutrophil infiltration in bronchoalveolar lavage fluid. tissue damage was assessed on the b ...	2011	21272064
infected cavitating pulmonary infarction.		2011	21276286
development of a dna microarray for detection and identification of legionella pneumophila and ten other pathogens in drinking water.	the safety and accessibility of drinking water are major concerns throughout the world. consumption of water contaminated with infectious agents, toxic chemicals or radiological hazards represents a significant health risk and is strongly associated with mortality. therefore, we have developed an oligonucleotide-based microarray using the sequences of 16s-23s rdna internal transcribed spacer regions (its) and the gyrase subunit b gene (gyrb) found in the most prevalent and devastating waterborne ...	2011	21276629
cross-transmission of klebsiella pneumoniae in two intensive care units: intra- and inter-hospital spread.		2011	21277048
[boerhaave's syndrome and pleural tuberculosis].	boerhaave's syndrome is a rare condition involving spontaneous rupture of the oesophagus. the diagnosis is difficult and the prognosis depends on early diagnosis and treatment.	2011	21277478
klebsiella pneumoniae outer membrane protein a is required to prevent the activation of airway epithelial cells.	outer membrane protein a (ompa) is a class of proteins highly conserved among the enterobacteriaceae family and throughout evolution. klebsiella pneumoniae is a capsulated gram-negative pathogen. it is an important cause of community-acquired and nosocomial pneumonia. evidence indicates that k. pneumoniae infections are characterized by a lack of an early inflammatory response. data from our laboratory indicate that k. pneumoniae cps helps to suppress the host inflammatory response. however, it ...	2011	21278256
exudate macrophages attenuate lung injury by the release of il-1 receptor antagonist in gram-negative pneumonia.	exudate macrophages are key players in host defense toward invading pathogens. their antiinflammatory and epithelial-protective potential in gram-negative pneumonia, however, remains elusive.	2011	21278303
synthesis of 2-mercaptobenzimidazole derivatives as potential anti-microbial and cytotoxic agents.	a series of novel 2-(1h-benzimidazol-2-ylsulfanyl)-n-(4-oxo-2-phenyl-thiazolidin-3yl)-acetamide 5a-j have been synthesized from various aldehydes and 2-(5-phenyl-[1,3,4]-oxadiazol-2-ylmethylsulfanyl)-1h-benzimidazole 6a-j from various benzoic acids. these compounds were screened for their in-vitro anti-bacterial activity against staphylococcus aureus and enterococcus faecalis as gram positive, klebsiella pneumoniae and escherichia coli as gram negative bacterial strains and for in-vitro anti-fun ...	2011	21280103
role of klebsiella pneumoniae lamb porin in antimicrobial resistance.	to investigate the contribution of lamb in klebsiella pneumoniae antimicrobial resistance, we determined the mics of various antibiotics and the frequency of mutation to increased cefoxitin or meropenem resistance of the strains csub10s (expressing only ompk36), csub10r (lacking ompk35 and ompk36), and their derived isogenic insertion-duplication mutants deficient in lamb. expression of lamb was indispensable in order for csub10s to lose ompk36 and become resistant to cefoxitin, while in csub10r ...	2011	21282437
novel vim metallo-beta-lactamase variant, vim-24, from a klebsiella pneumoniae isolate from colombia.	we report the emergence of a novel vim variant (vim-24) in a klebsiella pneumoniae isolate in colombia. the isolate displays mics for carbapenems below the resistance breakpoints, posing a real challenge for its detection. the blavim-24 gene was located within a class 1 integron carried on a large plasmid. further studies are needed to clarify its epidemiological and clinical impact.	2011	21282438
in vivo efficacy of the novel aminoglycoside achn-490 in murine infection models.	aminoglycosides are broad-spectrum antibiotics with particular clinical utility against life-threatening infections. as resistance to antibiotics, including aminoglycosides, continues to grow, there is a need for new and effective antimicrobial agents. achn-490 is a novel aminoglycoside in clinical development with activity against multidrug-resistant gram-negative and select gram-positive pathogens. here we assess the in vivo efficacy of achn-490 against a variety of common pathogens in two mur ...	2011	21282439
in vitro pharmacodynamics of simulated pulmonary exposures of tigecycline alone and in combination against klebsiella pneumoniae isolates producing a kpc carbapenemase.	multidrug-resistant klebsiella pneumoniae strains that produce a serine carbapenemase (kpc) are emerging worldwide, with few therapeutic options that retain consistent susceptibility. the objective of this study was to determine the effect of combination therapy with tigecycline versus tigecycline alone against kpc-producing isolates (kpc isolates). an in vitro pharmacodynamic model was used to simulate adult steady-state epithelial lining fluid concentrations of tigecycline (50 mg every 12 h) g ...	2011	21282442
klebsiella pneumoniae outer membrane porins ompk35 and ompk36 play roles in both antimicrobial resistance and virulence.	ompk35 and ompk36 are the major outer membrane porins of klebsiella pneumoniae. in this study, a virulent clinical isolate was selected to study the role of these two porins in antimicrobial resistance and virulence. the single deletion of ompk36 (?ompk36) resulted in mic shifts of cefazolin, cephalothin, and cefoxitin from susceptible to resistant, while the single deletion of ompk35 (?ompk35) had no significant effect. a double deletion of ompk35 and ompk36 (?ompk35/36) further increased these ...	2011	21282452
gallium-67 spect/ct for abdominal abscess.	an 80-year-old woman, who had suffered from end-stage renal disease under peritoneal dialysis, was presented with intermittent fever, leukocytosis, and elevated c-reactive protein for 4 months. she did not have symptom of abdominal pain. culture of ascites showed klebsiella pneumoniae. abdominal utrasonography was negative. whole-body gallium-67 imaging showed a segmental uptake mimicking bowels in right abdomen. spect/ct revealed the uptake in a soft tissue density beneath the abdominal wall in ...	2011	21285696
prevalence and role of efflux pump activity in ciprofloxacin resistance in clinical isolates of klebsiella pneumoniae.	we investigated the prevalence and role of efflux pump activity and possible drug influx resistance in ciprofloxacin susceptibility amongst 26 distinct clinical isolates of klebsiella pneumoniae of varying ciprofloxacin susceptibilities and known quinolone resistance-determining region (qrdr) genotypes. cellular [(14)c]ciprofloxacin accumulation patterns and the amount of cell-associated [(14)c]ciprofloxacin of mid-logarithmic phase cells were determined before and after challenging with the eff ...	2011	21286930
impact of ph on bacterial growth and activity of recent fluoroquinolones in pooled urine.	acidification of urine is widely recommended for prevention and treatment of urinary tract infections. we set out to describe the effect of modification of ph on bacterial growth of relevant bacteria as well as on activity of modern fluoroquinolones in urine in vitro. bacterial growth of escherichia coli atcc 25922 and klebsiella oxytoca atcc 700324 was determined in pooled human urine adjusted to ph levels between 5.0 and 8.0. minimal inhibitory concentrations (mics) and time-kill curves were p ...	2011	21288486
[myoplasty by reversal of the pectoris major muscle in the treatment of mediastinitis. a case report and review of the literature.]	mediastinitis are among the most dreadful infectious complications following cardiac surgery. their prognosis depends essentially on the precociousness of the diagnosis. in most of the cases, the medical treatment associated with an irrigation drainage is sufficient. but in case of severe sternal dehiscence, plastic surgery becomes necessary in order to fill up the loss with a well-vascularized tissue. we report the case of a 78-year-old patient, chronic bronchitic who presented, after a coronar ...	2011	21288617
rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis.	we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ...	2011	21289149
unusual cause of cerebral vasospasm after pituitary surgery.	cerebral vasospasm (cvs) was described in patients after trans-sphenoidal pituitary surgery due to intra-operative trauma of arteries or blood clots around the arteries of willis' circle. we consider that in the two presented cases the main cause of cvs in early postoperative period was meningitis. two patients with pituitary adenomas were operated with trans-sphenoidal approach. cvs developed in early postoperative period. meningitis was revealed in both cases. cvs regressed only after successf ...	2011	21290157
in vitro and in vivo properties of bal30376, a β-lactam and dual beta-lactamase inhibitor combination with enhanced activity against gram-negative bacilli that express multiple β-lactamases.	bal30376 is a triple combination comprising a siderophore monobactam, bal19764; a novel bridged monobactam, bal29880, which specifically inhibits class c β-lactamases; and clavulanic acid, which inhibits many class a and some class d β-lactamases. the mic(90) was ≤ 4 μg/ml (expressed as the concentration of bal19764) for most species of the enterobacteriaceae family, including strains that produced metallo-β-lactamases and were resistant to all of the other β-lactams tested. the mic(90) for sten ...	2011	21245441
international transfer of ndm-1-producing klebsiella pneumoniae from iraq to france.		2011	21245442
experimental phage therapy in treating klebsiella pneumoniae-mediated liver abscesses and bacteremia in mice.	intragastric inoculation of mice with klebsiella pneumoniae can cause liver abscesses, necrosis of liver tissues, and bacteremia. a newly isolated phage (φnk5) with lytic activity for k. pneumoniae was used to treat k. pneumoniae infection in an intragastric model. both intraperitoneal and intragastric administration of a single dose of φnk5 lower than 2 × 10(8) pfu at 30 min after k. pneumoniae infection was able to protect mice from death in a dose-dependent manner, but the efficacy achieved w ...	2011	21245450
intravenous colistin administration in neonates.	nosocomial infection due to multidrug-resistant gram-negative pathogens in intensive care units is a challenge for clinicians and microbiologists, and has led to resurgence of parenteral colistin use in the last decade. safety and efficacy data regarding intravenous colistin (colistimethate) use in neonates is sparse. we present our experience of efficacy and safety of colistimethate in the treatment of sepsis in critically sick term and preterm neonates.	2011	21245777
emergence of klebsiella pneumoniae carbapenemase-producing bacteria.	klebsiella pneumoniae carbapenemase (kpc)-producing bacteria are a group of emerging highly drug-resistant gram-negative bacilli causing infections associated with significant morbidity and mortality. once confined to outbreaks in the northeastern united states (us), they have spread throughout the us and most of the world. kpcs are an important mechanism of resistance for an increasingly wide range of gram-negative bacteria and are no longer limited to k pneumoniae. kpc-producing bacteria are o ...	2011	21119555
an in vitro comparative study determining bactericidal activity of stabilized chlorine dioxide and other oral rinses.	the study was conducted to determine the bactericidal activity of a stabilized chlorine dioxide oral rinse (closys oral rinse) compared to products currently available on the market.	2011	21290979
thrombocytes are effectors of the innate immune system releasing human beta defensin-3.	thrombocyte concentrate i.e. platelet-rich plasma (prp) has become a popular adjunct for many surgical procedures. it is believed to improve bone and soft tissue healing. recently antimicrobial effects of the autologous preparation were reported by several groups. in this study we investigated the antimicrobial effect of prp against gram-negative microbes which frequently cause severe complications in orthopaedic trauma surgery.	2011	21310405
bacterial species associated with sound and botrytis-infected grapes from a greek vineyard.	grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ...	2011	21315469
antimicrobial drug resistance in taiwan.	antimicrobial resistance is a major global health threat associated with high mortality rates and high medical costs. geographic variations in resistance profiles of bacterial and fungal pathogens have had a considerable impact on antimicrobial prescription. in taiwan, there is an alarmingly high prevalence of penicillin-resistant streptococcus pneumoniae, multidrug-resistant and extensively drug-resistant (xdr) pseudomonas aeruginosa and acinetobacter baumannii, extended-spectrum ß-lactamase-pr ...	2011	21316007
molecular epidemiology and resistance mechanisms involved in reduced susceptibility to amoxicillin/clavulanic acid in klebsiella pneumoniae isolates from a chronic care centre.	the aim of this work was to investigate the molecular epidemiology and mechanisms responsible for reduced susceptibility to amoxicillin/clavulanic acid (amc) amongst cefazolin-susceptible klebsiella pneumoniae isolates from patients admitted to a chronic care institution. in total, 51 (29.8%) of 171 k. pneumoniae isolates recovered between 2006 and 2008 were non-susceptible to amc, of which 45 were susceptible to cefazolin. nucleotide sequencing analysis revealed that 19 produced irt-11 and the ...	2011	21316198
synthesis, stereochemistry, antimicrobial evaluation and qsar studies of 2,6-diaryltetrahydropyran-4-one thiosemicarbazones.	a series of 2,6-diaryltetrahydropyran-4-one thiosemicarbazones (11-27) were synthesized and characterized for evaluation of potential antibacterial activity against staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, salmonella typhi, bacillus subtilis and klebsiella pneumonia and antifungal activity against cryptococcus neoformans, candida albicans, rhizopus sp., aspergillus niger and aspergillus flavus were evaluated. compounds 21 and 22 showed maximum inhibition potency at low co ...	2011	21316819
emergence of klebsiella pneumoniae st258 with kpc-2 in hong kong.		2011	21316928
containment of a country-wide outbreak of carbapenem-resistant klebsiella pneumoniae in israeli hospitals via a nationally implemented intervention.	during 2006, israeli hospitals faced a clonal outbreak of carbapenem-resistant klebsiella pneumoniae that was not controlled by local measures. a nationwide intervention was launched to contain the outbreak and to introduce a strategy to control future dissemination of antibiotic-resistant bacteria in hospitals.	2011	21317398
enhancement of 2,3-butanediol production by klebsiella oxytoca ptcc 1402.	optimal operating parameters of 2,3-butanediol production using klebsiella oxytoca under submerged culture conditions are determined by using taguchi method. the effect of different factors including medium composition, ph, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the taguchi method in three levels. based on these analyses the optimum concentrations of glucose, acetic acid, and succinic acid were found to be 6, 0.5, and 1.0 (% w/v), respect ...	2011	21318172
splenic abscess: a single institution study and review of the literature.	the aim of this study was to review our experience with splenic abscesses, with respect to the relevant aspects of splenic abscesses and treatment outcomes.	2011	21319348
transport and bacterial interactions of three bacterial strains in saturated column experiments.	the impact of bacteria-solid and bacteria-bacteria interactions on the transport of klebsiella oxytoca, burkholderia cepacia g4pr1, and pseudomonas sp. #5 was investigated in saturated sand column experiments (l = 114 mm; ø = 33 mm) under constant water velocities (~ 5 cm · h(-1)). bacterial strains were injected into the columns as pulses either individually, simultaneously, or successively. a one-dimensional mathematical model for advective-dispersive transport and for irreversible and reversi ...	2011	21319738
rapid genotyping of achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia isolates using melting curve analysis of rapd-generated dna fragments (mcrapd).	typing of bacteria is important for monitoring newly emerging pathogens and for examining local outbreaks. we evaluated the randomly amplified polymorphic dna technique in combination with melting curve analysis (mcrapd) of the amplified dna fragments to genotype isolates from five gram-negative species, i.e. achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia. by determining the melting temperature peaks of the ampl ...	2011	21320595
high prevalence of kpc-2-type carbapenemase coupled with ctx-m-type extended-spectrum beta-lactamases in carbapenem-resistant klebsiella pneumoniae in a teaching hospital in china.		2011	21321140
in vitro interactions of antimicrobial combinations with fosfomycin against kpc-2-producing klebsiella pneumoniae and protection of resistance development.	using time-kill methodology, we investigated the interactions of fosfomycin with meropenem or colistin or gentamicin against 17 genetically distinct klebsiella pneumoniae clinical isolates carrying blakpc-2. synergy was observed with meropenem or colistin against 64.7 and 11.8% of tested isolates, while the combination with gentamicin resulted in indifference. all studied combinations showed improved bactericidal activity, compared to fosfomycin alone and prevented the development of fosfomycin ...	2011	21321144
antimicrobial activity of cxa-101, a novel cephalosporin tested in combination with tazobactam against enterobacteriaceae, pseudomonas aeruginosa, and bacteroides fragilis strains having various resistance phenotypes.	cxa-101, a novel oxyimino-aminothiazolyl cephalosporin, cxa-201 (cxa-101 combined with tazobactam), and various comparators were susceptibility tested by broth microdilution methods against 1,301 well-characterized clinical strains collected worldwide, including ceftazidime-resistant members of the family enterobacteriaceae and klebsiella pneumoniae carbapenemase (kpc)- and extended-spectrum ß-lactamase (esbl)-producing strains of pseudomonas aeruginosa and bacteroides fragilis. cxa-201 was 2- t ...	2011	21321149
mutation in fucose synthesis gene of klebsiella pneumoniae affects capsule composition and virulence in mice.	the emerging pathogenicity of klebsiella pneumoniae (kp) is evident by the increasing number of clinical cases of liver abscess (la) due to kp infection. a unique property of kp is its thick mucoid capsule. the bacterial capsule has been found to contain fucose in kp strains causing la but not in those causing urinary tract infections. the products of the gmd and wcag genes are responsible for converting mannose to fucose in kp. a kp strain, kpl1, which is known to have a high death rate in infe ...	2011	21321319
bilateral klebsiella pneumoniae (k1 serotype) endogenous endophthalmitis as the presenting sign of disseminated infection.	the authors report a rare presentation of bilateral klebsiella pneumoniae endophthalmitis in an ambulatory patient without other known medical conditions. a 51-year-old chinese woman presented with severe bilateral reduction in vision, photophobia, pain, and eyelid swelling. hospital admission and evaluation revealed vitreous, blood, and urine cultures positive for k. pneumoniae (k1 serotype). additional work-up revealed endocarditis, multiple liver abscesses, brain abscesses, and left lobar pne ...	2011	21323188
comparison of in vitro activity of ertapenem with other carbapenems against extended-spectrum beta-lactamase-producing escherichia coli and kleibsella species isolated in a tertiary children's hospital.	the purpose of this study was to evaluate the susceptibility of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae clinical isolates to ertapenem in a tertiary pediatric care center in turkey.	2011	21323503
assessing the effect of different ultrasonic frequencies on bacterial viability using flow cytometry.	aims: this research investigated the effect of sonication at frequencies of 20, 40 and 580 khz and approximately the same acoustic intensity on the viability and declumping of two micro-organisms (escherichia coli and klebsiella pneumonia). methods and results: two analytical methods were employed; viable plate counts (cfu ml(-1) ) and flow cytometry to identify and quantify both live/viable and dead bacteria in the bulk liquid. flow cytometry results for e. coli and kl. pneumonia indicated a ...	2011	21324052
sequence analysis of a group of low molecular-weight plasmids carrying multiple is903 elements flanking a kanamycin resistance aph gene in salmonella enterica serovars.	a group of low molecular-weight cole1-like plasmids carrying the aph sequence type aph(ii) from three different salmonella serovars were sequenced. these plasmids carry two or more copies of is903 elements, with up to 21bp sequence differences to one another, two of which flank the aph gene. this group of plasmids did not appear to carry any known mobilization genes and instead carry three open reading frames encoding hypothetical proteins of unknown function possibly organized in an operon. the ...	2011	21324339
time-kill synergy tests of tigecycline combined with imipenem, amikacin, and ciprofloxacin against clinical isolates of multidrug-resistant klebsiella pneumoniae and escherichia coli.	this study evaluated the activity of tigecycline combined with imipenem, amikacin, and ciprofloxacin against clinical isolates of multidrug-resistant klebsiella pneumoniae and escherichia coli co-producing extended-spectrum ß-lactamases and acquired ampc ß-lactamases. broth microdilution tests were performed for cefotaxime, ceftazidime, cefepime, imipenem, amikacin, ciprofloxacin, and tigecycline. time-kill synergy studies were tested for tigecycline plus imipenem, tigecycline plus amikacin, and ...	2011	21325253
new delhi metallo-ß-lactamase (ndm-1)-producing klebsiella pneumoniae: case report and laboratory detection strategies.	the spread of antimicrobial resistance among enterobacteriaceae is a significant clinical threat. we report the first case of an enterobacteriaceae strain harboring the ndm-1 metallo-ß-lactamase in a pediatric patient in the united states. we describe strategies for the detection of this novel resistance mechanism encountered in an isolate of klebsiella pneumoniae.	2011	21325558
isolation and characterisation of kp34--a novel fkmv-like bacteriophage for klebsiella pneumoniae.	bacteriophage kp34 is a novel virus belonging to the subfamily autographivirinae lytic for extended-spectrum ß-lactamase-producing klebsiella pneumoniae strains. its biological features, morphology, susceptibility to chemical and physical agents, burst size, host specificity and activity spectrum were determined. as a potential antibacterial agent used in therapy, kp34 molecular features including genome sequence and protein composition were examined. phylogenetic analyses and clustering of kp34 ...	2011	21327407
isolation of a selected microbial consortium capable of degrading methyl parathion and p-nitrophenol from a contaminated soil site.	a bacterial consortium with the ability to degrade methyl parathion and p-nitrophenol, using these compounds as the only carbon source, was obtained by selective enrichment in a medium with methyl parathion. samples were taken from moravia, medellin; an area that is highly contaminated, owing to the fact that it was used as a garbage dump from 1974 to 1982. acinetobacter sp, pseudomonas putida, bacillus sp, pseudomonas aeruginosa citrobacter freundii, stenotrophomonas sp, flavobacterium sp, prot ...	2011	21328125
extended-spectrum ß-lactamases of escherichia coli and klebsiella pneumoniae screened by the vitek 2 system.	the vitek 2 automated system (biomérieux) is one of the most widely used instruments in clinical microbiology laboratories for the identification and evaluation of the susceptibility profiles of bacteria including the detection of extended-spectrum ß-lactamases (esbls) produced by escherichia coli, klebsiella pneumoniae and klebsiella oxytoca. currently, the clinical and laboratory standards institute recommends the use of esbl confirmatory tests in addition to standard susceptibility testing. i ...	2011	21330411
acute klebsiella pneumoniae interface keratitis after deep anterior lamellar keratoplasty.		2011	21331700
c-reactive protein and interleukin-6 responses for differentiating fungal and bacterial aetiology in late-onset neonatal sepsis.	fungal infections are increasingly frequent causes of neonatal sepsis (ns). this study examined the predictive value of the combined evaluation of the c-reactive protein (crp) and interleukin-6 (il-6) responses for differentiating fungal and bacterial aetiologies in patients with ns. from january to september 2007, neonates who were diagnosed with ns and had their crp and il-6 levels measured were selected. based on their blood culture results, the neonates were divided into two groups: group of ...	2011	19889172
detection of leptospira in urine using anti-leptospira-coated gold nanoparticles.	serological assays for antibody detection have been widely used for leptospirosis diagnosis. however, antibody is usually undetectable during the first week after infection. detection of leptospira dna can be done by pcr but this technique requires special equipments and the cost is still relatively high. here we demonstrate that gold nanoparticles can be used to facilitate leptospira detection. gold nanoparticles were coated with rabbit antibody specific to leptospira interrogans serovar bratis ...	2011	20015548
rosiglitazone ameliorates endotoxin-induced organ damage in conscious rats.	rosiglitazone is a peroxisome proliferator-activated receptor (ppar)-γ agonist. by inhibiting nuclear factor κb (nf-κb), it decreases tumor necrosis factor-α (tnf-α) and interleukin 6 (il-6) and has an anti-inflammatory effect. endotoxin shock can induce the production of several inflammatory mediators such as tnf-α and il-6, leading to multiple organ dysfunction and death. we investigated the effects of rosiglitazone (.3 mg/kg, intravenous administration) on the physiologic attributes and cytok ...	2011	20034949
development of a loop-mediated isothermal amplification assay for the detection of mycobacterium bovis.	bovine tuberculosis caused by mycobacterium bovis is an important zoonosis. in this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. the technique will be of value in the clinical and field-based diagnosis of m. bovis and can differentiate it from other bacteria such as corynebacterium diphtheriae, streptococcus pneumoniae, β-haemolytic streptococci, pseudomonas aeruginosa, yersinia pseudotuberculosis, stap ...	2011	20138787
intercontinental spread from israel to colombia of a kpc-3-producing klebsiella pneumoniae strain.	in 2008, an increase in the prevalence of carbapenem-resistant klebsiella pneumoniae was noted in a 286-bed tertiary case hospital in colombia, where 84 patients (32 infected and 52 colonized) had positive cultures. the identified index patient came from israel for a liver transplantation. high level carbapenem resistance was observed. polymyxin b and tigecycline were the only two antibiotics that remained active. pcr-restriction fragment length polymorphism analysis and sequencing revealed blak ...	2011	20219078
metallo-β-lactamases among enterobacteriaceae from routine samples in an italian tertiary-care hospital and long-term care facilities during 2008.	the emergence of metallo-β-lactamase (mbl)-producing enterobacteriaceae is a serious public health concern. producers have been repeatedly isolated from patients and long-term care facility (ltcf) residents around bolzano, and we sought to assess their prevalence and clinical impact. all routine enterobacteriaceae isolates from a bolzano tertiary-care hospital and associated long-term care facilities in 2008 (n = 5500) were screened for mbls, with case details reviewed for the source patients. i ...	2011	20345467
an outbreak of neonatal sepsis presenting with exanthematous rash caused by klebsiella pneumoniae.	newborns often present with skin conditions ranging from transient benign to severe. an occasional cause of neonatal exanthemic rash is infection due to gram-positive bacteria, but gram-negative infection is uncommon. we describe an outbreak of sepsis in 10 neonates over a 2-week period, caused by klebsiella pneumoniae in a level iii neonatal intensive care unit (nicu). seven babies developed an exanthematous maculopapular rash. molecular analysis by pulsed-field gel electrophoresis showed that ...	2011	20370956
bacteriological quality of drinking water in nyala, south darfur, sudan.	the objective of this study was to determine the bacterial contaminations in drinking water in nyala city, south darfur, sudan with special reference to the internally displaced people camps (idps). two hundred and forty water samples from different sites and sources including bore holes, hand pumps, dug wells, water points, water reservoir and household storage containers were collected in 2009. the most probable number method was used to detect and count the total coliform, faecal coliform and ...	2011	20480392
cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract.	cadmium (cd²(+)), a naturally occurring heavy metal, is an important environmental pollutant and a potent toxicant to bacteria. the gastrointestinal (gi) tract microflora has a marked capacity to cope with the increased load of ingested metals. however, heavy metals may have harmful effects on git microflora. under the conditions of experimental exposure to cadmium, changes in the population of intestinal microflora in healthy mice were examined. five experimental groups received 23 to 50 mg kg⁻ ...	2011	20488844
kpc-2 producing klebsiella pneumoniae and escherichia coli co-infection in a catheter-related infection.	we describe the first report of simultaneous blood infection with kpc-2 producing klebsiella pneumoniae and escherichia coli in a brazilian patient. we highlight the importance of implementing efficient infection control measures to limit the spread of these phenotypes in a hospital setting.	2011	20491828
risk factors for late onset gram-negative infections: a case-control study.	to determine the incidence, mortality and risk factors for neonatal late onset gram-negative sepsis and meningitis (logns).	2011	20538712
screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth.	a total of 216 bacterial strains were isolated from rice rhizospheric soils in northern thailand. the bacterial strains were initially tested for solubilization of inorganic phosphate, indole acetic acid (iaa) production, selected strains were then tested for optimized conditions for iaa production and whether these caused stimulatory effects on bean and maize seedling growth. it was found that all strains had solubilized inorganic phosphate (p), but only 18.05% produced iaa. the best iaa produc ...	2011	20552360
x-ray diagnosis of fatal fournier's gangrene caused by klebsiella pneumoniae.		2011	20558485
emphysematous cystitis: a rare cause of gross hematuria.	emphysematous cystitis is a relatively rare infectious condition of the urinary bladder. the mortality rate is high if the diagnosis is delayed or if the treatment is inadequate. we present an uncommon case of emphysematous cystitis and highlight the risk factors for this disease. an 81-year-old man with a past medical history of type 2 diabetes and a central pontine infarction presented to the emergency department due to gross hematuria. computed tomography (ct) and plain radiography revealed l ...	2011	18993014
anti-microbial activity of urine after ingestion of cranberry: a pilot study.	we explore the anti-microbial activity of urine specimens after the ingestion of a commercial cranberry preparation. twenty subjects without urinary infection, off antibiotics and all supplements or vitamins were recruited. the study was conducted in two phases: in phase 1, subjects collected the first morning urine prior to ingesting 900 mg of cranberry and then at 2, 4 and 6 h. in phase 2, subjects collected urine on 2 consecutive days: on day 1 no cranberry was ingested (control specimens), o ...	2010	18955308
mhbr, a lysr-type regulator involved in 3-hydroxybenzoate catabolism via gentisate in klebsiella pneumoniae m5a1.	in klebsiella pneumoniae m5a1, mhbtdhim genes are involved in 3-hydroxybenzoate catabolism via the gentisate pathway. mhbr, which encodes a lysr-type transcriptional regulator, is divergently transcribed from the mhb structural genes. mhbr was found to be necessary for the expression of catabolic genes. transcriptional studies demonstrated that the mhb structural genes are transcribed as an operon. the promoters of mhbr and the mhb operon are sigma(70)-type and overlap with each other. 5' deleti ...	2010	18801651
[the role of microflora in etiology of chronic tonsillitis].	analysis of the results of a bacteriological study involving 218 patients with various forms of chronic tonsillitis (ct) has demonstrated that they were significantly different in terms of the seeding rate of beta-hemolytic group a streptococci (bhas). specifically, these microorganisms were detected in patients with paratonsillar abscess 2.5 times as frequently as in those with uncomplicated forms of chronic tonsilitis. in other words, the former group is at risk of developing such concomitant ...	2010	20559241
an unusual case of recurrent endogenous klebsiella endophthalmitis.		2010	20559330
[epidemiology of patients harboring extended-spectrum beta-lactamase-producing enterobacteriaceae (esble), on admission].	the incidence of extended-spectrum beta-lactamase-producing enterobacteria (esble) has regularly increased over the last few years. however, little is known about epidemiology of esble carriers in france. the objective of this study was to determine the esble carriers or infected patients profile, identified within 48 hours following hospital admission.	2010	20554140
[characterization of the variable region within class 1 integrons in klebsiella pneumoniae nosocomials strains].	integrons are responsible for the capture and dissemination of resistance genes in gram-negative bacteria.	2010	20556335
the cyclotide cycloviolacin o2 from viola odorata has potent bactericidal activity against gram-negative bacteria.	to determine the antibacterial activity of small cyclic plant proteins, i.e. cyclotides, and the importance of the surface exposed charged residues for activity.	2010	20558471
pulmonary amyloidosis in sjogren's syndrome: a case report and systematic review of the literature.	amyloidosis is a heterogeneous group of disorders characterized by misfolding of extracellular proteins. the two most common forms are light-chain (al) and reactive (aa) amyloidosis. pulmonary amyloidosis secondary to sjogren's syndrome (ss) is rare, and there is a paucity of data on its clinical and radiological features. we describe the case of a 41-year-old woman with diffuse septal pulmonary al (lambda) amyloidosis related to ss, in the absence of systemic amyloidosis. a systematic search fo ...	2010	20546191
dramatic increase of third-generation cephalosporin-resistant e. coli in german intensive care units: secular trends in antibiotic drug use and bacterial resistance, 2001 to 2008.	the objective of the present study was to analyse secular trends in antibiotic consumption and resistance data from a network of 53 intensive care units (icus).	2010	20546564
pharmacokinetic-pharmacodynamic profiling of four antimicrobials against gram-negative bacteria collected from shenyang, china.	to examine common antimicrobial regimens used in eradicating certain nosocomial gram-negative pathogens and determine which ones are likely to be the most suitable as empirical choices in shenyang, china.	2010	20546625
hypermucoviscosity associated with klebsiella pneumoniae-mediated invasive syndrome: a prospective cross-sectional study in taiwan.	to investigate the association between klebsiella pneumoniae-mediated invasive syndrome and underlying diseases in patients and/or k. pneumoniae characteristics, including the rmpa, rmpa2, and maga genes, capsular polysaccharide (cps) k(1) or k(2) serotypes, hypermucoviscosity (hv) phenotype, and extended-spectrum beta-lactamase (esbl) production.	2010	20547084
outbreak caused by a multi-resistant klebsiella pneumoniae strain of new sequence type st341 carrying new genetic environments of aac(6')-ib-cr and qnrs1 genes in a neonatal intensive care unit in spain.	an outbreak due to a klebsiella pneumoniae clone occurred in a neonatal intensive care unit of a spanish hospital in which three newborns were infected (all with gestational age ≤29 weeks; two of them died) and seven were colonized (gestational age >32 weeks; none died). one k. pneumoniae strain per patient was further characterized. the 10 strains showed an indistinguishable pulsed-field-gel-electrophoresis pattern, were typed in the phylogenetic group kpi and were ascribed into a new sequence ...	2010	20547103
complete nucleotide sequence of klebsiella pneumoniae multidrug resistance plasmid pkp048, carrying blakpc-2, bladha-1, qnrb4, and arma.	the klebsiella pneumoniae multidrug resistance plasmid pkp048 was completely sequenced. this plasmid carries several important resistance determinants, such as bla(kpc-2), bla(dha-1), qnrb4, and arma, which confer resistance to carbapenems, cephalosporins, fluoroquinolones, and aminoglycosides, respectively. analysis of the finished 151,188-bp sequence data revealed 163 putative genes, 108 of which were assigned functions such as replication, stable inheritance, antibiotic resistance, a mobile e ...	2010	20547789
evaluation of a dna microarray, the check-points esbl/kpc array, for rapid detection of tem, shv, and ctx-m extended-spectrum beta-lactamases and kpc carbapenemases.	extended-spectrum beta-lactamases (esbls) and klebsiella pneumoniae carbapenemases (kpc carbepenemases) have rapidly emerged worldwide and require rapid identification. the check-points esbl/kpc array, a new commercial system based on genetic profiling for the direct identification of esbl producers (shv, tem, and ctx-m) and of kpc producers, was evaluated. well-characterized gram-negative rods (enterobacteriaceae, pseudomonas aeruginosa, acinetobacter baumannii) expressing various ss-lactamases ...	2010	20547813
effect of cumin (cuminum cyminum) seed essential oil on biofilm formation and plasmid integrity of klebsiella pneumoniae.	seeds of the cumin plant (cuminum cyminum l.) have been used since many years in iranian traditional medicine. we assessed the effect of cumin seed essential oil on the biofilm-forming ability of klebsiella pneumoniae strains and on the integrity of a native resistance plasmid dna from k. pneumoniae isolates, treated with essential oil. antibacterial coaction between the essential oil and selected antibiotic disks were determined for inhibiting k. pneumoniae. the essential oil of the cumin seeds ...	2010	20548937
antibiotic susceptibility of intra-abdominal infection isolates from indian hospitals during 2008.	a total of 542 clinical isolates of aerobic gram-negative bacilli from intra-abdominal infections were collected during 2008 from seven hospitals in india participating in the study for monitoring antimicrobial resistance trends (smart). isolates were from various infection sources, the most common being gall bladder (30.1 %) and peritoneal fluid (31.5 %), and were mostly hospital-associated isolates (70.8 %) as compared to community-acquired (26.9 %). the most frequently isolated pathogens were ...	2010	20538892
sepsis and cardiomyopathy as rare clinical manifestations of pheochromocytoma--two case report studies.	the clinical manifestation of pheochromocytomas is highly variable and can closely resemble numerous clinical conditions. here, we report on two cases of patients with pheochromocytoma, which manifested as sepsis or cardiomyopathy. the first patient initially presented with bacterial urosepsis due to klebsiella oxytoca. despite effective antibiotic therapy, the patient developed recurring fever accompanied by hypertension. the inconsistency between therapy-refractory hypertension and fever indic ...	2010	20539976