Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| natural antibiotic susceptibility of enterobacter spp., with special reference to enterobacter aerogenes and enterobacter intermedius strains. | the natural susceptibility to 71 antibiotics of 44 strains of enterobacter aerogenes and 12 strains of enterobacter intermedius was examined using a microdilution procedure in isosensitest broth (for all strains) and cation-adjusted mueller hinton broth (for some strains). both species were naturally sensitive or sensitive and intermediate to tetracyclines, all tested aminoglycosides, several penicillins and cephalosporins, carbapenems, aztreonam, quinolones, folate-pathway inhibitors, chloramph ... | 2002 | 12462424 |
| effectiveness of electrolyzed water as a sanitizer for treating different surfaces. | the effectiveness of electrolyzed (eo) water at killing enterobacter aerogenes and staphylococcus aureus in pure culture was evaluated. one milliliter (approximately 10(9) cfu/ml) of each bacterium was subjected to 9 ml of eo water or control water (eo water containing 10% neutralizing buffer) at room temperature for 30 s. inactivation (reduction of > 9 log10 cfu/ ml) of both pathogens occurred within 30 s after exposure to eo water containing approximately 25 or 50 mg of residual chlorine per l ... | 2002 | 12182480 |
| method for estimation of low outer membrane permeability to beta-lactam antibiotics. | the outer membrane of gram-negative bacteria plays a major role in beta-lactam resistance as it slows down antibiotic entry into the periplasm and therefore acts in synergy with beta-lactamases and efflux systems. up to now, the quantitative estimation of low outer membrane permeability by the method of zimmermann and rosselet was difficult because of the secreted and cell surface-associated beta-lactamases. the method presented here uses the acylation of a highly sensitive periplasmic penicilli ... | 2002 | 12183245 |
| risk factors for infection and molecular typing in patients in the intensive care unit colonized with nosocomial enterobacter aerogenes. | to determine the frequency of colonization by enterobacter aerogenes in patients in the intensive care unit (icu) for more than 48 hours and to evaluate the risk factors for infection in patients colonized by this bacteria. | 2002 | 12186211 |
| prba, a gene coding for an esterase hydrolyzing parabens in enterobacter cloacae and enterobacter gergoviae strains. | the new gene prba encodes an esterase responsible for the hydrolysis of the ester bond of parabens in enterobacter cloacae strain em. this gene is located on the chromosome of strain em and was cloned by several pcr approaches. the prba gene codes for an immature protein of 533 amino acids, the first 31 of which represent a proposed signal peptide yielding a mature protein of a putative molecular mass of 54.6 kda. this enzyme presents analogies with other type b carboxylesterases, mainly of euka ... | 2002 | 12193616 |
| [urinary tract infections due to wide spectrum beta lactamase producing enterobacteriaceae]. | the aim of this study was to specify the characteristics of enterobacterial urinary infections producing wide spectrum beta-lactamase (wsbl) and the management strategies for these patients infected in geriatric wards. | 2002 | 12212511 |
| impact of previous use of antibiotics on development of resistance to extended-spectrum cephalosporins in patients with enterobacter bacteremia. | this retrospective study was conducted to determine the risk factors for resistance to extended-spectrum cephalosporins (escs) and to examine the influence of previous use of an aminoglycoside with an esc on resistance to escs in patients with enterobacter bacteremia from january 1991 through december 2000. a total of 423 episodes of enterobacter bacteremia among 414 patients were documented during the 10-year study period. three hundred thirty-two (78%) isolates were enterobacter cloacae, 72 (1 ... | 2002 | 12226687 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ... | 2002 | 12071094 |
| the first archaeal atp-dependent glucokinase, from the hyperthermophilic crenarchaeon aeropyrum pernix, represents a monomeric, extremely thermophilic rok glucokinase with broad hexose specificity. | an atp-dependent glucokinase of the hyperthermophilic aerobic crenarchaeon aeropyrum pernix was purified 230-fold to homogeneity. the enzyme is a monomeric protein with an apparent molecular mass of about 36 kda. the apparent k(m) values for atp and glucose (at 90 degrees c and ph 6.2) were 0.42 and 0.044 mm, respectively; the apparent v(max) was about 35 u/mg. the enzyme was specific for atp as a phosphoryl donor, but showed a broad spectrum for phosphoryl acceptors: in addition to glucose, whi ... | 2002 | 12374829 |
| utilization of l-ascorbate by escherichia coli k-12: assignments of functions to products of the yjf-sga and yia-sgb operons. | escherichia coli k-12 can ferment l-ascorbate. the operon encoding catabolic enzymes in the utilization of l-ascorbate (ula) has been identified; this operon of previously unknown function had been designated the yif-sga operon. three enzymes in the pathway that produce d-xylulose 5-phosphate have been functionally characterized: 3-keto-l-gulonate 6-phosphate decarboxylase (ulad), l-xylulose 5-phosphate 3-epimerase (ulae), and l-ribulose 5-phosphate 4-epimerase (ulaf). several products of the yi ... | 2002 | 11741871 |
| novel alkylsulfatases required for biodegradation of the branched primary alkyl sulfate surfactant 2-butyloctyl sulfate. | recent reports show that contrary to common perception, branched alkyl sulfate surfactants are readily biodegradable in standard biodegradability tests. we report here the isolation of bacteria capable of biodegrading 2-butyloctyl sulfate and the identification of novel enzymes that initiate the process. enrichment culturing from activated sewage sludge yielded several strains capable of growth on 2-butyloctyl sulfate. of these, two were selected for further study and identified as members of th ... | 2002 | 11772605 |
| ethnobotanical and antimicrobial investigation on some species of terminalia and combretum (combretaceae) growing in tanzania. | an ethnobotanical investigation on the medicinal uses of some species of terminalia and combretum (combretaceae) was carried out in mbeya, tanzania during a 5-weeks field expedition. of the sixteen species collected, combretum fragrans f. hoffm., combretum molle g. don., combretum psidioides welw., combretum zeyheri sond., terminalia kaiserana f. hoffm. and terminalia sericea burch ex. dc. have medical applications against various bacterial infections, such as gonorrhoea and syphilis, and agains ... | 2002 | 11801378 |
| crystallization and preliminary x-ray structure determination of jack bean urease with a bound antibody fragment. | urease allows organisms to use exogenous and internally generated urea as a nitrogen source, by catalyzing the hydrolysis of urea to form ammonia and carbon dioxide. urease may also participate in the systemic nitrogen-transport pathways and possibly acts as a toxic defence protein. jack bean urease (jbu) was the first nickel-metalloenzyme identified and was crystallized as early as 1926. despite this, the structure has not yet been determined. an antibody fragment, fv, that has a high affinity ... | 2002 | 11807281 |
| mar operon involved in multidrug resistance of enterobacter aerogenes. | we determined the sequence of the entire marrab operon in enterobacter aerogenes. it is functionally and structurally analogous to the escherichia coli operon. the overexpression of e. aerogenes mara induces a multidrug resistance phenotype in a susceptible strain, demonstrated by a noticeable resistance to various antibiotics, a decrease in immunodetected porins, and active efflux of norfloxacin. | 2002 | 11897595 |
| tem derivative-producing enterobacter aerogenes strains: dissemination of a prevalent clone. | tem-24 (caz-6) extended-spectrum beta-lactamase (esbl) was detected in 1988 in clermont-ferrand, france, in klebsiella pneumoniae (bla(tem-24)) and enterobacter aerogenes (bla(tem-24b)), and since 1994, a tem-24-producing e. aerogenes clonal strain has been observed elsewhere in the country. to determine if the spread of this clonal strain was restricted to tem-24-producing e. aerogenes strains, 84 e. aerogenes strains (non-tem/shv-producing strains, tem-1- or -2-producing strains, and different ... | 2002 | 11897606 |
| epidemiology of extended-spectrum beta-lactamase-producing enterobacter isolates in a spanish hospital during a 12-year period. | fifteen enterobacter clinical isolates (11 enterobacter cloacae isolates, 3 enterobacter aerogenes isolates, and 1 enterobacter gergoviae isolate), representing 0.4% of all enterobacter isolates recovered in our hospital from 1989 to 2000, were suspected of harboring an extended-spectrum beta-lactamase (esbl). these isolates were recovered from 14 different patients. esbls were transferred by conjugation into an escherichia coli recipient strain. pulsed-field gel electrophoresis (pfge) revealed ... | 2002 | 11923338 |
| evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies. | phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ... | 2002 | 11931166 |
| prevalence of extended spectrum beta-lactamases among enterobacteriaceae: an italian survey. | the prevalence of extended-spectrum beta-lactamase (esbl) production by consecutive non-repeated isolates of enterobacteriaceae was determined over a 6-month period. a total of 8015 strains were isolated from ten italian laboratories and 509 (6.3%) of these were designated esbl producers from the results of a double-disk synergy test. escherichia coli was the most isolated microrganism, followed by klebsiella pneumoniae and proteus mirabilis. providencia stuartii (28.1%) was the most frequently ... | 2002 | 11932144 |
| [enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital]. | to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms. | 2002 | 11939120 |
| polyphosphate produced in recombinant escherichia coli confers mercury resistance. | an escherichia coli strain was generated by fusion of a mera-deleted broad-spectrum mer operon from pseudomonas k-62 with a bacterial polyphosphate kinase gene (ppk) from klebsiella aerogenes in vector puc119. a large amount of the ppk-specified polyphosphate was identified in the mercury-induced bacterium with the fusion plasmid designated pmkb18 but not in the cells without mercury induction. these results suggest that the synthesis of polyphosphate as well as the expression of the mer genes i ... | 2002 | 11958934 |
| nb2001, a novel antibacterial agent with broad-spectrum activity and enhanced potency against beta-lactamase-producing strains. | enzyme-catalyzed therapeutic activation (ecta) is a novel prodrug strategy to overcome drug resistance resulting from enzyme overexpression. beta-lactamase overexpression is a common mechanism of bacterial resistance to beta-lactam antibiotics. we present here the results for one of the beta-lactamase ecta compounds, nb2001, which consists of the antibacterial agent triclosan in a prodrug form with a cephalosporin scaffold. unlike conventional beta-lactam antibiotics, where hydrolysis of the bet ... | 2002 | 11959554 |
| [post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria]. | as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ... | 2002 | 11977920 |
| pyogenic liver abscess -- 20 years' experience. comparison of results of treatment in two periods. | our aim is to give an audit of our experience over the past two decades in the form of a retrospective study. | 2002 | 11981681 |
| cloning and sequencing of enterobacter aerogenes ompc-type osmoporin linked to carbapenem resistance. | using outbreak-related strains of enterobacter aerogenes, we cloned and sequenced ompk39, the structural gene coding for outer membrane protein ompk39. its lack of expression was closely associated with a phenotype exhibiting low-level carbapenem resistance. detailed alignment of the predicted amino acid sequence revealed that ompk39 is a member of the ompc subclass of enterobacterial porins, with the highest degree of homology to klebsiella pneumoniae ompk36. based on a computerized alignment i ... | 2002 | 12008919 |
| development of a sensitive and specific enzyme-linked immunosorbent assay for detecting and quantifying cmy-2 and shv beta-lactamases. | polyclonal rabbit antibodies against shv-1 and cmy-2 beta-lactamases were produced and characterized, and enzyme-linked immunosorbent assays (elisas) were developed. immunoblots revealed that the anti-shv-1 antibody recognized shv-1 but did not recognize tem-1, k-1, oxa-1, or any ampc beta-lactamase tested. the anti-cmy-2 antibody detected escherichia coli cmy-2, enterobacter cloacae p99, klebsiella pneumoniae act-1, and the ampc beta-lactamases of enterobacter aerogenes, morganella morganii, an ... | 2002 | 12037047 |
| susceptibility of enterobacteriaceae to beta-lactam agents and fluoroquinolones: a 3-year survey in france. | to assess trends in the susceptibility to beta-lactam agents and to fluoroquinolones of clinically relevant enterobacteriaceae isolated over a 3-year period in 14 french hospital laboratories. | 2002 | 12047412 |
| three-dimensional quantitative structure-activity relationship and comparative molecular field analysis of dipeptide hydroxamic acid helicobacter pylori urease inhibitors. | a homology model of helicobacter pylori urease was developed by using the crystal structure of urease from klebsiella aerogenes (ec 3.5.1.5) as a template. the acetohydroxamic acid moiety was docked into the active pocket of the enzyme model, followed by relaxation of the complex by use of molecular dynamics. the resulting conformation was used as a template to construct 24 potential dipeptide hydroxamic acid inhibitors with which comparative molecular field analysis (comfa) was performed. the r ... | 2002 | 12121941 |
| the acrab-tolc efflux pump contributes to multidrug resistance in the nosocomial pathogen enterobacter aerogenes. | we identified the genes encoding the acra-acrb-tolc efflux pump in enterobacter aerogenes and constructed acrab and tolc mutants from a multidrug-resistant isolate. both derivatives were more susceptible to antibiotics than the parental strain. sequence analysis and complementation experiments revealed that the multidrug-resistant isolate is an acrr mutant. | 2002 | 12121946 |
| but-1: a new member in the chromosomal inducible class c beta-lactamases family from a clinical isolate of buttiauxella sp. | an atypical enterobacteriaceae strain with a beta-lactam susceptibility pattern of inducible cephalosporinase was isolated in tenon hospital (paris, france) from a patient's skull wound infection. identifications by the api-50che biochemical system and 16s rrna gene sequencing concluded that it was a member of the buttiauxella genus. the bla gene was cloned and sequenced. the deduced translated product was a 383-amino acid protein (but-1) with 75-78% identity with the chromosomal ampc beta-lacta ... | 2002 | 12127496 |
| helicobacter pylori may survive ampicillin treatment in the remnant stomach. | helicobacter pylori (h. pylori) is a gram-negative curved rod-like or spiral bacterium that chronically infects the human gastric mucosa, and is a major risk factor for gastritis, gastric and duodenal ulcer and adenocarcinoma of the stomach. after partial gastrectomy, some patients may have persistent h. pylori infection for five years or more. in this study, we detected three bacteria, i.e., klebsiella pneumoniae, enterobacter aerogenes, and escherichia coli, in the gastric juice of patients wi ... | 2002 | 12139018 |
| acute bilateral emphysematous pyelonephritis successfully managed by medical therapy alone: a case report and review of the literature. | bilateral emphysematous pyelonephritis is a life threatening condition usually occurring in diabetics. management of this condition has traditionally been aggressive and surgery is considered mandatory. however, this is itself a hazardous intervention in a septic, unstable patient with circulatory or liver failure. when bilateral disease is present, the need for long-term dialysis is obviously unavoidable. | 2002 | 12057010 |
| [total and fecal coliforms, some enterobacteria staphylococcus sp. and moulds in salads for hot dogs sold in maracay, venezuela]. | fast food sold on the streets are good vehicle to transmit gastrointestinal diseases to consumers because they are highly manipulated. the vegetables are a good media for pathogens growth. raw vegetables salads for hot dog sold in maracay were studied. total and fecal coliforms and staphylococcus sp. were quantified by the most probable number technique, coliforms and staphylococcus isolated were partially identified. also the mould population was quantified and identified. microbial growth was ... | 2001 | 12012562 |
| relationship between teat-end callosity and occurrence of clinical mastitis. | a longitudinal study in 15 herds, with a total of 2157 cows, was conducted to examine the relationship between teat-end callosity (tec) and the incidence of clinical mastitis. during the 1.5-yr study period, clinical mastitis was diagnosed by the farmers based on clinical signs. teat-end callosity was scored every month according to a teat-end callosity classification system, which discriminates between teat-end callosity thickness (tect) and roughness (tecr). differences in tect between healthy ... | 2001 | 11814022 |
| antimicrobial susceptibility of respiratory isolates of enterobacteriaceae and staphylococcus aureus in italy: incidence and trends over the period 1997-1999. | the antibiotic susceptibility of members of the family enterobacteriaceae and of staphylococcus aureus strains isolated from the respiratory tract was assessed over the period 1997-1999 as part of the italian epidemiological observatory survey sponsored by the smith-kline foundation. a standardised method was used to determine the mics of 22 antibiotics against isolates of klebsiella pneumoniae (n=870), escherichia coli (n=684), enterobacter cloacae (n=342), enterobacter aerogenes (n=187) and se ... | 2001 | 11837636 |
| rapid identification of ralstonia eutropha strain ch34 using monoclonal antibodies. | ralstonia eutropha strain ch34 (formerly alcaligenes eutrophus ch34) is an aerobic gram-negative and facultative chemolithotrophic bacteria with plasmid-bound resistance to heavy metals. the presence of ralstonia eutropha strain ch34 is an indication of environmental heavy metals pollution. the major purpose of this work was to produce monoclonal antibodies (mabs) against the metal transport outer membrane proteins. in this way, bacteria outer membranes, grown with or without iron, were purified ... | 2001 | 11839250 |
| roles of glutamate synthase, gltbd, and gltf in nitrogen metabolism of escherichia coli and klebsiella aerogenes. | mutants of escherichia coli and klebsiella aerogenes that are deficient in glutamate synthase (glutamate-oxoglutarate amidotransferase [gogat]) activity have difficulty growing with nitrogen sources other than ammonia. two models have been proposed to account for this inability to grow. one model postulated an imbalance between glutamine synthesis and glutamine degradation that led to a repression of the ntr system and the subsequent failure to activate transcription of genes required for the us ... | 2001 | 11673431 |
| pcr analyses of trna intergenic spacer, 16s-23s internal transcribed spacer, and randomly amplified polymorphic dna reveal inter- and intraspecific relationships of enterobacter cloacae strains. | pcr analysis of trna intergenic spacer (tdna-pcr) and of the 16s-23s internal transcribed spacer (its-pcr) and random amplified polymorphic dna (rapd) analysis were evaluated for their usefulness in characterization of enterobacter cloacae strains isolated from both clinical origins and vaccine microbial contamination. tdna-pcr presented specific and reproducible patterns for enterobacter sakazakii atcc 29004, enterobacter aerogenes atcc 13048, and enterobacter cloacae atcc 13047 and 23355 that ... | 2001 | 11682499 |
| benzoate fermentation by the anaerobic bacterium syntrophus aciditrophicus in the absence of hydrogen-using microorganisms. | the anaerobic bacterium syntrophus aciditrophicus metabolized benzoate in pure culture in the absence of hydrogen-utilizing partners or terminal electron acceptors. the pure culture of s. aciditrophicus produced approximately 0.5 mol of cyclohexane carboxylate and 1.5 mol of acetate per mol of benzoate, while a coculture of s. aciditrophicus with the hydrogen-using methanogen methanospirillum hungatei produced 3 mol of acetate and 0.75 mol of methane per mol of benzoate. the growth yield of the ... | 2001 | 11722901 |
| shellac formulations to reduce epiphytic survival of coliform bacteria on citrus fruit postharvest. | survival of the coliform bacteria enterobacter aerogenes and escherichia coli was monitored in a neutral carboxymethylcellulose formulation and in shellac formulations with various ph and concentrations of ethanol and the preservative paraben; populations were subsequently measured from the surface of citrus fruit coated with these formulations. numbers of the two bacteria increased over 24 h from 10(6) cfu/ml to approximately 10(8) cfu/ml in the carboxymethylcellulose solution, but over this ti ... | 2001 | 11726155 |
| [purification and properties of chitinase from enterobacter aerogenes]. | a bacterium producing chitinase was isolated from the dead body of gymephorap ruoergensis. a chitinase was isolated from the culture of e. aerogenes and purified by means of ammonium sulfate precipitation, deae-cellulose column chromatography, and sephadex g-100 column gel filtration. the purified chitinase showed homogeneity on the native polyacrylamide gel electrophoresis. its molecular weight was estimated to be about 42.5 kd by sds-page. the optimum ph and temperature for hydrolysis of chiti ... | 2001 | 12549194 |
| comparative in vitro activity of isepamicin and other antibiotics against gram-negative bacilli from intensive care units (icu) in belgium. | the in vitro activity of isepamicin was compared to that of amikacin, gentamicin, cefepime, ciprofloxacin and meropenem against gram-negative bacilli isolated from patients hospitalized in the intensive care unit (icu) of 11 belgian university or general hospitals between november 1998 and july 1999. the minimal inhibitory concentrations (mic) for 1087 non-duplicate, consecutive aerobic gram-negative isolates, including 798 enterobacteriaceae and 289 non-fermenters, were determined by e-test for ... | 2001 | 11774843 |
| efficiency of cefepime in postoperative meningitis attributable to enterobacter aerogenes. | 2001 | 11379596 | |
| intra- and inter-generic plasmid-mediated spread of cephalosporin and aminoglycoside resistance amongst klebsiella aerogenes k41 and other enterobacteria. | klebsiella aerogenes k41, resistant to third generation cephalosporins and aminoglycosides, was isolated from clinical samples of 153 in-patients. blood cultures accounted for 24 (15.7%) of isolates. the mic(90) of ceftazidime for the isolates of 84 patients was >512 mg/l and was reduced to 2.0 by 4 mg/l of clavulanic acid, but only to 64 by 4 mg/l of sulbactam. isolates of k. aerogenes k41 produced extended-spectrum beta-lactamase (esbl) shv-5 and tem-1, identified by isoelectric focusing. plas ... | 2001 | 11165116 |
| evaluation of the contribution of isolation precautions in prevention and control of multi-resistant bacteria in a teaching hospital. | from february 1999 to january 2000, a control programme to prevent the spread multi-resistant bacteria (mrb) was implemented in a french teaching hospital. this programme focused on methicillin-resistant staphylococcus aureus (mrsa) and enterobacteriaceae producing extended-spectrum beta-lactamases (esbl), and was based on the application of barrier precautions (washing hands with antiseptic soaps, wearing disposable gloves and gowns, identifying mrb carriers). no changes in antibiotic policy oc ... | 2001 | 11170775 |
| new pyridoquinoline derivatives as potential inhibitors of the fluoroquinolone efflux pump in resistant enterobacter aerogenes strains. | enterobacter aerogenes, one of the most frequently isolated nosocomial pathogens in france, is exhibiting increasing multidrug resistance mechanisms associated with a change in membrane permeability. for drugs of the quinolone family, mutations in the target and active efflux play a prominent role in the resistance. we report here the effect of several pyridoquinoline derivatives that restore a noticeable fluoroquinolone accumulation to resistant strains that overexpress the mara activator. stud ... | 2001 | 11689091 |
| evaluation of ppk-specified polyphosphate as a mercury remedial tool. | to evaluate the utility of polyphosphate kinase gene (ppk)-specified polyphosphate in mercury remediation, a fusion plasmid, pmk27, with ppk from klebsiella aerogenes and mercury transport genes, mert and merp, from pseudomonas k-62, was constructed. the transcription and translation of ppk, mert and merp were found to be mercury-inducible. the ppk-specified polyphosphate was identified in cells preinduced by hg2+, but not in cells without mercury induction, suggesting that the synthesis of poly ... | 2001 | 11767115 |
| catabolite repression of the citrate fermentation genes in klebsiella pneumoniae: evidence for involvement of the cyclic amp receptor protein. | klebsiella pneumoniae is able to grow anaerobically with citrate as a sole carbon and energy source by a fermentative pathway involving the na(+)-dependent citrate carrier cits, citrate lyase, and oxaloacetate decarboxylase. the corresponding genes are organized in the divergent citc and cits operons, whose expression is strictly dependent on the citrate-sensing cita-citb two-component system. evidence is provided here that the citrate fermentation genes are subject to catabolite repression, sin ... | 2001 | 11514506 |
| dual effects of ionic strength on klebsiella aerogenes urease: ph-dependent activation and inhibition. | 2001 | 11594743 | |
| [spread of enterobacteriaceae producing broad-spectrum beta-lactamase and the development of their incidence over a 16-month period in a university hospital center]. | enterobacteriaceae producing extended-spectrum beta-lactamases (esble) constitute with methicillin-resistant staphylococcus aureus the main multiresistant bacteria recovered in french hospitals. our objectives were to evaluate these esble diffusion in our teaching hospital and to follow their incidence during a 16-month period, whereas a control programme (barrier precautions) had been implemented in the beginning of 1999. this study was conducted in a teaching hospital containing 1800 beds, fro ... | 2001 | 11642012 |
| role for outer membrane cytochromes omca and omcb of shewanella putrefaciens mr-1 in reduction of manganese dioxide. | shewanella putrefaciens mr-1 can use a wide variety of terminal electron acceptors for anaerobic respiration, including certain insoluble manganese and iron oxides. to examine whether the outer membrane (om) cytochromes of mr-1 play a role in mn(iv) and fe(iii) reduction, mutants lacking the om cytochrome omca or omcb were isolated by gene replacement. southern blotting and pcr confirmed replacement of the omca and omcb genes, respectively, and reverse transcription-pcr analysis demonstrated los ... | 2001 | 11133454 |
| antimicrobial properties of garlic oil against human enteric bacteria: evaluation of methodologies and comparisons with garlic oil sulfides and garlic powder. | the antimicrobial effects of aqueous garlic extracts are well established but those of garlic oil (go) are little known. methodologies for estimating the antimicrobial activity of go were assessed and go, go sulfide constituents, and garlic powder (gp) were compared in tests against human enteric bacteria. test methodologies were identified as capable of producing underestimates of go activity. antimicrobial activity was greater in media lacking tryptone or cysteine, suggesting that, as for alli ... | 2001 | 11133485 |
| urea utilization in the phototrophic bacterium rhodobacter capsulatus is regulated by the transcriptional activator ntrc. | the phototrophic nonsulfur purple bacterium rhodobacter capsulatus can use urea as a sole source of nitrogen. three transposon tn5-induced mutations (xan-9, xan-10, and xan-19), which led to a ure(-) phenotype, were mapped to the uref and urec genes, whereas two other tn5 insertions (xan-20 and xan-22) were located within the ntrc and ntrb genes, respectively. as in klebsiella aerogenes and other bacteria, the genes encoding urease (ureabc) and the genes required for assembly of the nickel metal ... | 2001 | 11133958 |
| alternative pathways for siroheme synthesis in klebsiella aerogenes. | siroheme, the cofactor for sulfite and nitrite reductases, is formed by methylation, oxidation, and iron insertion into the tetrapyrrole uroporphyrinogen iii (uro-iii). the cysg protein performs all three steps of siroheme biosynthesis in the enteric bacteria escherichia coli and salmonella enterica. in either taxon, cysg mutants cannot reduce sulfite to sulfide and require a source of sulfide or cysteine for growth. in addition, cysg-mediated methylation of uro-iii is required for de novo synth ... | 2001 | 11114933 |
| methionine-to-cysteine recycling in klebsiella aerogenes. | in the enteric bacteria escherichia coli and salmonella enterica, sulfate is reduced to sulfide and assimilated into the amino acid cysteine; in turn, cysteine provides the sulfur atom for other sulfur-bearing molecules in the cell, including methionine. these organisms cannot use methionine as a sole source of sulfur. here we report that this constraint is not shared by many other enteric bacteria, which can use either cysteine or methionine as the sole source of sulfur. the enteric bacterium k ... | 2001 | 11114934 |
| regulation of carbon and electron flow in clostridium butyricum vpi 3266 grown on glucose-glycerol mixtures. | the metabolism of clostridium butyricum was manipulated at ph 6.5 and in phosphate-limited chemostat culture by changing the overall degree of reduction of the substrate using mixtures of glucose and glycerol. cultures grown on glucose alone produced only acids (acetate, butyrate, and lactate) and a high level of hydrogen. in contrast, when glycerol was metabolized, 1,3-propanediol became the major product, the specific rate of acid formation decreased, and a low level of hydrogen was observed. ... | 2001 | 11160107 |
| the mtl genes and the mannitol-1-phosphate dehydrogenase from klebsiella pneumoniae kay2026. | the mtl operon of klebsiella pneumoniae kay2026 (formerly aerobacter aerogenes 1033-5p14) was shown to contain as the promoter-proximal gene mtla, encoding a d-mannitol-specific enzyme ii transporter (iicba(mtl)). this gene is followed by mtld, coding for a mannitol-1-phosphate dehydrogenase (mtld, 382 amino acid residues), and mtlr (mtlr, 195 amino acid residues) coding for a putative repressor, gene mtlr overlaps the termination codon of mtld. the dna and protein sequences are highly similar t ... | 2001 | 11164312 |
| crystallization of full-length cysb of klebsiella aerogenes, a lysr-type transcriptional regulator. | cysb is a positive regulator of transcription of genes involved in cysteine biosynthesis in gram-negative bacteria and belongs to the large family of lysr-type transcriptional regulators. the full-length protein from klebsiella aerogenes has been crystallized from solutions containing peg 8000 in the presence and in the absence of the inducer n-acetylserine by the method of vapour diffusion in hanging drops. for the complexed protein different crystal forms appear in the same drops. | 2001 | 11173473 |
| molecular characterization of global regulatory rna species that control pathogenicity factors in erwinia amylovora and erwinia herbicola pv. gypsophilae. | rsmb(ecc) specifies a nontranslatable rna regulator that controls exoprotein production and pathogenicity in soft rot-causing erwinia carotovora subsp. carotovora. this effect of rsmb(ecc) rna is mediated mostly by neutralizing the function of rsma(ecc), an rna-binding protein of e. carotovora subsp. carotovora, which acts as a global negative regulator. to determine the occurrence of functional homologs of rsmb(ecc) in non-soft-rot-causing erwinia species, we cloned the rsmb genes of e. amylovo ... | 2001 | 11222584 |
| specific growth rate plays a critical role in hydrogen peroxide resistance of the marine oligotrophic ultramicrobacterium sphingomonas alaskensis strain rb2256. | the marine oligotrophic ultramicrobacterium sphingomonas alaskensis rb2256 has a physiology that is distinctly different from that of typical copiotrophic marine bacteria, such as vibrio angustum s14. this includes a high level of inherent stress resistance and the absence of starvation-induced stress resistance to hydrogen peroxide. in addition to periods of starvation in the ocean, slow, nutrient-limited growth is likely to be encountered by oligotrophic bacteria for substantial periods of tim ... | 2001 | 11229924 |
| national epidemiologic surveys of enterobacter aerogenes in belgian hospitals from 1996 to 1998. | two national surveys were conducted to describe the incidence and prevalence of enterobacter aerogenes in 21 belgian hospitals in 1996 and 1997 and to characterize the genotypic diversity and the antimicrobial resistance profiles of clinical strains of e. aerogenes isolated from hospitalized patients in belgium in 1997 and 1998. twenty-nine hospitals collected 10 isolates of e. aerogenes, which were typed by arbitrarily primed pcr (ap-pcr) using two primers and pulsed-field gel electrophoresis. ... | 2001 | 11230400 |
| antimicrobial activity of honokiol and magnolol isolated from magnolia officinalis. | the antimicrobial activity of honokiol and magnolol, the main constituents of magnolia officinalis was investigated. the antimicrobial activity was assayed by the agar dilution method using brain heart infusion medium and the minimum inhibitory concentration (mic) were determined for each compound using a twofold serial dilution assay. the results showed that honokiol and magnolol have a marked antimicrobial effect (mic = 25 microg/ml) against actinobacillus actinomycetemcomitans, porphyromonas ... | 2001 | 11268114 |
| growth inhibition caused by overexpression of the structural gene for glutamate dehydrogenase (gdha) from klebsiella aerogenes. | two linked mutations affecting glutamate dehydrogenase (gdh) formation (gdh-1 and rev-2) had been isolated at a locus near the trp cluster in klebsiella aerogenes. the properties of these two mutations were consistent with those of a locus containing either a regulatory gene or a structural gene. the gdha gene from k. aerogenes was cloned and sequenced, and an insertion mutation was generated and shown to be linked to trp. a region of gdha from a strain bearing gdh-1 was sequenced and shown to h ... | 2001 | 11274137 |
| functional characterisation of urease accessory protein g (ureg) from potato. | the activation of the nickel metalloenzyme urease is a complex process. in bacteria, several urease accessory proteins are essential for incorporation of nickel into the active centre of urease. comparatively little is known about the activation process and the proteins involved in plants. we cloned five different cdnas encoding isoforms of urease accessory protein g (ureg) in potato. the 5'-coding region of these cdnas is highly polymorphic within solanum tuberosum ssp. tuberosum, containing ma ... | 2001 | 11289508 |
| cloning and characterisation of male in burkholderia pseudomallei. | no recombinant protein is available for serodiagnosis or skin test in the diagnosis of melioidosis. this report describes the cloning of the male gene, which encodes an immunogenic protein of burkholderia pseudomallei. bi-directional dna sequencing of male revealed that the gene contained a single open reading frame encoding 416 amino acid residues with a predicted molecular mass of 44.4 kda. blast analysis showed that the putative protein encoded by male is homologous to the maltose-binding pro ... | 2001 | 11289518 |
| quantification and variability analysis of bacterial cross-contamination rates in common food service tasks. | this study investigated bacterial transfer rates between hands and other common surfaces involved in food preparation in the kitchen. nalidixic acid-resistant enterobacter aerogenes b199a was used as a surrogate microorganism to follow the cross-contamination events. samples from at least 30 different participants were collected to determine the statistical distribution of each cross-contamination rate and to quantify the natural variability associated with that rate. the transfer rates among ha ... | 2001 | 11198444 |
| h(2)o(2) produced by viridans group streptococci may contribute to inhibition of methicillin-resistant staphylococcus aureus colonization of oral cavities in newborns. | in an accompanying report, we showed that viridans group streptococci may prevent methicillin-resistant staphylococcus aureus (mrsa) colonization of the oral cavities of newborns. in the present study, we investigated the mechanism of prevention in vitro. most viridans group streptococci had bacteriocin-like activity and killed mrsa, burkholderia cepacia, enterobacter aerogenes, and pseudomonas aeruginosa; however, escherichia coli, enterobacter cloacae, and candida albicans were resistant. the ... | 2001 | 11317240 |
| evidence of in vivo transfer of a plasmid encoding the extended-spectrum beta-lactamase tem-24 and other resistance factors among different members of the family enterobacteriaceae. | the epidemiological study of several multidrug-resistant enterobacteriaceae isolated from five patients demonstrated in vivo dissemination of a 100-kb plasmid encoding the extended-spectrum beta-lactamase tem-24 from a clonal strain of enterobacter aerogenes to different strains of klebsiella pneumoniae, escherichia coli, proteus vulgaris, proteus mirabilis, and serratia marcescens. | 2001 | 11326030 |
| in vitro activities of ertapenem (mk-0826) against recent clinical bacteria collected in europe and australia. | ertapenem (mk-0826, l-749,345) is a 1-beta-methyl carbapenem with a long serum half-life. its in vitro activity was determined by broth microdilution against 3,478 bacteria from 12 centers in europe and australia, with imipenem, cefepime, ceftriaxone, and piperacillin-tazobactam used as comparators. ertapenem was the most active agent tested against members of the family enterobacteriaceae, with mics at which 90% of isolates are inhibited (mic(90)s) of < or =1 microg/ml for all species. ertapene ... | 2001 | 11353638 |
| changes in the disintegration properties of some brands of paracetamol tablets inoculated with four bacterial species. | four most common brands of paracetamol (4-aceta-midophenol) tablets were examined for the changes in their disintegration properties after inoculation with staphylococcus aureus, bacillus cereus, klebsiella aerogenes and pseudomonas aeruginosa and incubating for 5 weeks. the disintegration times varied from one brand to the other, reaching maximum values of 72 min., 82 min., 110 min. and 120 min. for s. aureus, b. cereus, p. aeruginosa and klebsiella aerogenes, respectively. all brands of parace ... | 2001 | 11370281 |
| coexistence of shv-4- and tem-24-producing enterobacter aerogenes strains before a large outbreak of tem-24-producing strains in a french hospital. | in 1996, a monitoring program was initiated at the teaching hospital of amiens, france, and carried out for 3 years. all extended-spectrum beta-lactamase (esbl)-producing enterobacter aerogenes isolates recovered from clinical specimens were collected for investigation of their epidemiological relatedness by pulsed-field gel electrophoresis and enterobacterial repetitive intergenic consensus pcr (eric-pcr) and determination of the type of esbl harbored by isoelectric focusing and dna sequencing. ... | 2001 | 11376055 |
| phenotypic variability of beta-glucoside utilization and its correlation to pathogenesis process in a few enteric bacteria. | utilization of beta-glucosides is markedly variable in the members of the family enterobacteriaceae. the results presented here provide molecular clues for evolutionary events that resulted in the phenotypic variability seen amongst the members of these species. the genomic hybridization of selected enterobacteriaceae members with the escherichia coli bgl and cel genes resulted in detection of a complete homolog of the bgl and cel operons in shigella sonnei, a member that is evolutionarily close ... | 2001 | 11377874 |
| glove barriers to bacterial cross-contamination between hands to food. | human hands are an important source of microbial contamination of foods. however, published data on the effectiveness of handwashing and glove use in a foodservice setting are limited. bacterial transfer through foodservice quality gloves was quantified using nalidixic acid-resistant enterobacter aerogenes (a nonpathogenic surrogate with attachment characteristics similar to salmonella). five transfer rates were determined: chicken to bare hand, chicken to hand through gloves, bare hand to lettu ... | 2001 | 11403136 |
| [isolation and characterization of injured coliforms from the drinking water distribution network of la plata, argentina]. | we screened the la plata drinking water distribution network for fecal and total coliform bacterial indicator by purification procedures, cultivating 66 membrane-filtered samples from the two networks on m-t7 agar. subterranean and river-derived water yielded 13 and 18 confirmed gram-negative bacillus isolates, with 54% and 72% representing total coliforms, respectively. those from the former source were klebsiella oxytoca, enterobacter agglomerans, and enterobacter aerogenes and from the latter ... | 2001 | 11407022 |
| phylogenetic analyses of klebsiella species delineate klebsiella and raoultella gen. nov., with description of raoultella ornithinolytica comb. nov., raoultella terrigena comb. nov. and raoultella planticola comb. nov. | the phylogenetic relationships of the type strains of 9 klebsiella species and 20 species from 11 genera of the family enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16s rrna and rpob genes. the sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. the phylogenetic inference of the sequence comparison confirmed that the genus klebsiella is heterogeneous and composed of species which form three clusters that ... | 2001 | 11411716 |
| two-dimensional electrophoresis and peptide mass fingerprinting of bacterial outer membrane proteins. | many bacterial outer membrane proteins (omps) are missing from two-dimensional (2-d) gel proteome maps. recently, we developed a technique for 2-d electrophoresis (2-de) of escherichia coli omps using alkaline ph incubation for isolation of omps, followed by improved solubilization conditions for array by 2-de using immobilized ph gradients. in this report, we expanded our study, examining protein components from the outer membranes of two enteric bacteria, salmonella typhimurium and klebsiella ... | 2001 | 11425224 |
| comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study. | the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ... | 2001 | 11448564 |
| a new mechanism of antibiotic resistance in enterobacteriaceae induced by a structural modification of the major porin. | in enterobacter aerogenes, multidrug resistance involves a decrease in outer membrane permeability associated with changes in an as yet uncharacterized porin. we purified the major porin from the wild-type strain and a resistant strain. we characterized this porin, which was found to be an ompc/ompf-like protein and analysed its pore-forming properties in lipid bilayers. the porin from the resistant strain was compared with the wild-type protein and we observed (i) that its single-channel conduc ... | 2001 | 11454211 |
| oxidation of phenolate siderophores by the multicopper oxidase encoded by the escherichia coli yack gene. | a gene (yack) encoding a putative multicopper oxidase (mco) was cloned from escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. the purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (epr) spectra consistent with the presence of type 1, type 2, and type 3 copper centers. the strong optical a(610) (e(610) = 10,890 m(-1) cm(-1)) and copper stoichiometry were taken a ... | 2001 | 11466290 |
| resistance to antibiotics in injured coliforms isolated from drinking water. | we studied the antibiotic sensitivity of injured coliforms isolated from drinking water of la plata, argentina. the antibiotic sensitivity test by the agar diffusion method were proved in: klebsiella oxytoca (14 strains), enterobacter aerogenes (4 strains) and enterobacter cloacae genomic group 3 (14 strains). we found that while these impaired total coliforms were sensitive to piperacillin-tazobactam (taz), netilmicin (ntl), ofloxacin (oflx), and norfloxacin (nflx) (100%), they had resistant to ... | 2001 | 11471826 |
| uv raman spectral intensities of e. coli and other bacteria excited at 228.9, 244.0, and 248.2 nm. | resonance raman spectral intensities per average bacterial cell have been measured quantitatively for gram-negative escherichia coli, citrobacter freundii, and enterobacter aerogenes, as well as gram-positive bacillus subtilis and staphylococcus epidermidis. spectra have been obtained from cultures in the lag, log, and stationary growth phases excited in turn by 228.9, 244.0, and 248.2 nm light. although raman spectral peak positions (cm(-1)) excited by a given wavelength are very similar for al ... | 2001 | 11476245 |
| etiologies of the urinary tract infections in a yemeni city. | to determine the causative agents of urinary tract infection in yemeni patients in sana'a city, and to determine the antibiotic susceptibilities of these agents in vitro. | 2001 | 11479641 |
| multiple organ failure in septic patients. | multiple organ failure (mof) is the main cause of death in icus, especially affecting septic patients. it is strongly related to number of systems with failure, type of system involved, risk factors such as age, previous chronic diseases, delayed or inadequate resuscitation, persistent infection, immune suppression, and others. the prognoses is worse for patients rather than in elective or emergency surgical patients. the objective of this article is to provide data from our university teaching ... | 2001 | 11506772 |
| complete nucleotide sequence and organization of the atrazine catabolic plasmid padp-1 from pseudomonas sp. strain adp. | the complete 108,845-nucleotide sequence of catabolic plasmid padp-1 from pseudomonas sp. strain adp was determined. plasmid padp-1 was previously shown to encode atza, atzb, and atzc, which catalyze the sequential hydrolytic removal of s-triazine ring substituents from the herbicide atrazine to yield cyanuric acid. computational analyses indicated that padp-1 encodes 104 putative open reading frames (orfs), which are predicted to function in catabolism, transposition, and plasmid maintenance, t ... | 2001 | 11544232 |
| novel gene cassettes and integrons. | an increase in multiresistant enterobacteriaceae was observed at one of the departments of the university medical center utrecht. nine different integrons and 17 gene cassettes were found, including the new gene cassette aada8. this cassette was highly related to aada3 and aada2. in addition, an unknown promoter sequence was found for two integrons. | 2001 | 11557503 |
| a matter of bacterial life and death. | over 50 years ago, standard microbiological methods were established for determining whether bacterial cells were dead or alive. recently there has been a flurry of reports suggesting that bacteria may exist in an eclipsed state, escaping detection by standard methods. whether there really is such a state is of more than academic interest, considering the implications for public health. the ensuing debate has been unusually energetic for the normally cultured community of microbiologists. | 2001 | 11559589 |
| electrochemical biosensor array for the identification of microorganisms based on lectin-lipopolysaccharide recognition. | rapid identification of bacterial strains remains a well-known problem in applied medicine and, for viable pathogens, is an important diagnostic goal. we have investigated an electrochemical biosensor array, in which transduction is based on respiratory cycle activity measurements, where the microorganism's native respiratory chain is interrupted with non-native external oxidants. the selective biochemical recognition agents employed in this study are lectins that, once immobilized, recognize an ... | 2001 | 11569815 |
| crystal structure of klebsiella aerogenes uree, a nickel-binding metallochaperone for urease activation. | uree is proposed to be a metallochaperone that delivers nickel ions to urease during activation of this bacterial virulence factor. wild-type klebsiella aerogenes uree binds approximately six nickel ions per homodimer, whereas h144*uree (a functional c-terminal truncated variant) was previously reported to bind two. we determined the structure of h144*uree by multi-wavelength anomalous diffraction and refined it to 1.5 a resolution. the present structure reveals an hsp40-like peptide-binding dom ... | 2001 | 11591723 |
| [utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients]. | the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ... | 2001 | 11594009 |
| microbial hydrogen production from sweet potato starch residue. | clostridium butyricum could produce hydrogen from a sweet potato starch residue upon supplementation of nitrogen sources. a repeated batch culture using a mixed culture of c. butyricum and enterobacter aerogenes produced hydrogen with a yield of 2.4 mol h2/mol glucose under a controlled culture ph of 5.25 in a medium consisting of the sweet potato starch residue and 0.1% polypepton without addition of any reducing agents. rhodobacter sp. m-19 produced hydrogen from the supernatant of the culture ... | 2001 | 16232947 |
| acid phosphatase/phosphotransferases from enteric bacteria. | we have investigated the enzymatic phosphorylation of nucleosides and found that morganella morganii phoc acid phosphatase exhibits regioselective pyrophosphate (pp(i))-nucleoside phosphotransferase activity. in this study, we isolated genes encoding an acid phosphatase with regioselective phosphotransferase activity (ap/ptase) from providencia stuartii, enterobacter aerogenes, escherichia blattae and klebsiella planticola, and compared the primary structures and enzymatic characteristics of the ... | 2001 | 16233057 |
| in vitro susceptibility of nosocomial isolates against a new aminoglycoside isepamicin. | isepamicin is a new aminoglycoside with a spectrum of activity similar to amikacin with an advantage of possessing a high level of stability against different aminoglycoside modifying enzymes. the in vitro susceptibility of nosocomial isolates obtained from patients admitted to the all india institute of medical sciences, new delhi during october-december, 1999 was tested against isepamicin and compared with other aminoglycosides. | 2001 | 21910285 |
| antimicrobial activities of ferulago essential oils. | essential oils from ferulago asparagifolia boiss., f. galbanifera (miller) w. koch, f. humilis boiss. (endemic), f. trachycarpa boiss. growing in turkey were evaluated against 15 microorganisms for their antifungal and antibacterial activity using an agar tube dilution and microdilution broth susceptibility assay, respectively. the essential oil compositions were investigated by gc/ms. inhibitory effects against escherichia coli, enterobacter aerogenes, candida albicans, gaeumannomyces graminis ... | 2000 | 11204191 |
| [comparative antibacterial activity of cefotaxime, ceftazidime and cefepime with regard to different strains of enterobacter aerogenes selected for their resistance to third generation cephalosporins]. | after being confronted with the isolation in our laboratory of numerous antibiotic-multiresistant enterobacter aerogenes strains, we studied the in vitro antimicrobial activity of cefotaxime, ceftazidime, and cefepime alone or in association with sulbactam. for that, we selected 67 isolates according to their low level of susceptibility to cefotaxime. first, we deduced from a synergy test in presence of clavulanic acid and cloxacillin the production of an extended spectrum beta-lactamase (esbl) ... | 2000 | 11204925 |
| synthesis of novel heterobranched beta-cyclodextrins from alpha-d-mannosylmaltotriose and beta-cyclodextrin by the reverse action of pullulanase, and isolation and characterization of the products. | alpha-d-mannosyl-maltotriose (man-g3) were synthesized from methyl alpha-mannoside and maltotriose by the transfer action of alpha-mannosidase. (man-g3)-betacd and (man-g3)2-betacd were produced in about 20% and 4% yield, respectively when aerobacter aerogenes pullulanase (160 units per 1 g of man-g3) was incubated with the mixture of 1.6 m man-g3 and 0.16 m betacd at 50 degrees c for 4 days. the reaction products, (man-g3)-betacd were separated to three peaks by hplc analysis on a ymc-pack a-32 ... | 2000 | 11193409 |
| isolation and insecticidal effects of some bacteria from euproctis chrysorrhoea l. (lepidoptera: lymantriidae). | in this study, we investigated the bacterial pathogens of euproctis chrysorrhoea and tested for their insecticidal activities. based on colony color and morphology, four isolates were determined. according to morphological, physiological and biochemical characters of the isolates, they were identified as enterobacter aerogenes, lactobacillus sp., bacillus thuringiensis and micrococcus luteus. the insecticidal effects of these bacterial isolates on third-fourth instar larvae of euproctis chrysorr ... | 2000 | 11293654 |
| tem-24 extended-spectrum beta-lactamase-producing enterobacter aerogenes: long-term clonal dissemination in french hospitals. | to investigate interstrain relatedness of tem-24-producing enterobacter aerogenes clinical strains isolated between 1993 and 1998 in 10 french hospitals from nine areas by pulsed-field gel electrophoresis (pfge) and plasmid patterns. | 2000 | 11168140 |
| changing pattern of antimicrobial susceptibility of organisms causing community acquired urinary tract infections. | to assess common organisms causing urinary treat infection (uti) in this community and to see antimicrobial susceptibility pattern of these isolates. | 2000 | 11126812 |
| antibiotic resistance and policy in belgium. | antibiotic resistance is increasing worldwide, also in belgium. a few examples to illustrate this problem: s. pneumoniae are becoming increasingly resistant to penicillin and erythromycin; resistance of s. pyogenes to erythromycin is increasing year-by-year; salmonella and campylobacter are becoming more and more resistant to the fluoroquinolones. in hospitals, the problem of methicillin-resistant staphylococcus aureus appears to be decreasing, but two clones of ceftazidime-resistant enterobacte ... | 2000 | 11144690 |
| identification of the gene encoding sulfopyruvate decarboxylase, an enzyme involved in biosynthesis of coenzyme m. | the products of two adjacent genes in the chromosome of methanococcus jannaschii are similar to the amino and carboxyl halves of phosphonopyruvate decarboxylase, the enzyme that catalyzes the second step of fosfomycin biosynthesis in streptomyces wedmorensis. these two m. jannaschii genes were recombinantly expressed in escherichia coli, and their gene products were tested for the ability to catalyze the decarboxylation of a series of alpha-ketoacids. both subunits are required to form an alpha( ... | 2000 | 10940029 |
| phg1p is a nine-transmembrane protein superfamily member involved in dictyostelium adhesion and phagocytosis. | to identify the molecular mechanisms involved in phagocytosis, we generated random insertion mutants of dictyostelium discoideum and selected two mutants defective for phagocytosis. both represented insertions in the same gene, named phg1. this gene encodes a polytopic membrane protein with an n-terminal lumenal domain and nine potential transmembrane segments. homologous genes can be identified in many species; however, their function is yet to be elucidated. disruption of phg1 caused a selecti ... | 2000 | 10944536 |