Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| a case-control study of men with non-gonococcal urethritis at auckland sexual health service: rates of detection of mycoplasma genitalium. | previous studies have identified mycoplasma genitalium as a cause of urethritis in men. as there is no new zealand data, a case-control study was conducted to determine whether this organism is a significant cause of urethritis in men presenting to auckland sexual health service. | 2010 | 20152101 |
| is screening asymptomatic men who have sex with men for urethral gonorrhoea worthwhile? | we determined the prevalence of symptomatic and asymptomatic urethral gonorrhoea among men who have sex with men (msm) at our australian sexual health clinic. asymptomatic msm are screened using the roche amplicor((r)) pcr, whereas culture is used for symptomatic msm. we analysed data from all msm tested for urethral gonorrhoea from march 2006 to july 2008. among the 4453 asymptomatic msm, there were two (0.04%) diagnoses of urethral gonorrhoea, compared with 38 (3.13%) among 1213 symptomatic ms ... | 2010 | 20152104 |
| preventable deaths in sickle-cell anaemia in african children. | 2010 | 20152540 | |
| clinical and microbiological findings at sites treated with orthodontic fixed appliances in adolescents. | fixed orthodontic appliances can alter the subgingival microbiota. our aim was to compare the subgingival microbiota and clinical parameters in adolescent subjects at sites of teeth treated with orthodontic bands with margins at (obm) or below the gingival margin (obsm), or with brackets (obr). | 2010 | 20152679 |
| organization of the electron transfer chain to oxygen in the obligate human pathogen neisseria gonorrhoeae: roles for cytochromes c4 and c5, but not cytochrome c2, in oxygen reduction. | although neisseria gonorrhoeae is a prolific source of eight c-type cytochromes, little is known about how its electron transfer pathways to oxygen are organized. in this study, the roles in the respiratory chain to oxygen of cytochromes c(2), c(4), and c(5), encoded by the genes ccca, cyca, and cycb, respectively, have been investigated. single mutations in genes for either cytochrome c(4) or c(5) resulted in an increased sensitivity to growth inhibition by excess oxygen and small decreases in ... | 2010 | 20154126 |
| the genetic structure of neisseria meningitidis populations in cuba before and after the introduction of a serogroup bc vaccine. | we investigated the population genetics in collections of meningococci sampled in cuba during the period 1983-2005, thereby covering a period before and after the introduction of an antimeningococcal b-c vaccine. a total of 163 case isolates and 210 isolates from healthy carriers were characterized by multilocus sequence typing (mlst) and sequence determination of pora, porb and feta genes. a total of 56 sequence types (sts) including 28 new sts were identified among these isolates. the analysis ... | 2010 | 20156598 |
| spectrum of activity, mutation rates, synergistic interactions, and the effects of ph and serum proteins for fusidic acid (cem-102). | fusidic acid (cem-102) is a steroidal antimicrobial agent with focused gram-positive activity that acts by preventing bacterial protein synthesis via interacting with elongation factor g. a collection of 114 wild-type isolates (> 80 species) was used to define the contemporary limits of fusidic acid spectrum against gram-positive and gram-negative species. reference broth microdilution and anaerobic agar dilution methods were performed. modifications of standardized test methods included adding ... | 2010 | 20159376 |
| neisseria meningitidis with reduced susceptibility to quinolones in singapore. | 2010 | 20007332 | |
| regulatory role of the misr/s two-component system in hemoglobin utilization in neisseria meningitidis. | outer membrane iron receptors are some of the major surface entities that are critical for meningococcal pathogenesis. the gene encoding the meningococcal hemoglobin receptor, hmbr, is both independently transcribed and transcriptionally linked to the upstream gene hemo, which encodes a heme oxygenase. the misr/s two-component system was previously determined to regulate hmbr transcription, and its hemo and hmbr regulatory mechanisms were characterized further here. the expression of hemo and hm ... | 2010 | 20008531 |
| universal immunization of infants against neisseria meningitidis: addressing the remaining unmet medical need in the prevention of meningitis and septicemia. | effective immunization policies have markedly decreased the incidence of many lethal infectious diseases of childhood, including diphtheria, pertussis, and poliomyelitis, among others. in industrialized countries, relatively recent success in combating meningitis and sepsis has come with the implementation of universal immunization of infants against haemophilus influenzae type b (hib) and streptococcus pneumoniae. these universal immunization programs have reduced disease incidence and related ... | 2010 | 20009513 |
| hnco-based measurement of one-bond amide 15n-1h couplings with optimized precision. | a pair of 3d hnco-based experiments have been developed with the aim of optimizing the precision of measurement of (1)j(nh) couplings. both pulse sequences record (1)j(nh) coupling evolution during the entire constant time interval that (15)n magnetization is dephasing or rephasing with respect to the directly bonded (13)c' nucleus, with (15)n(13)c' multiple quantum coherence maintained during the (13)c' evolution period. the first experiment, designed for smaller proteins, produces an apparent ... | 2010 | 20012159 |
| a hypothetical explanatory model for meningococcal meningitis in the african meningitis belt. | despite much progress in surveillance and biological research, no explanation exists to date for the epidemic pattern of meningitis in the african meningitis belt, which is required to mathematically model the impact of vaccine strategies or to predict epidemics. this paper presents a hypothetical explanatory model for epidemic meningococcal meningitis. four incidence patterns are defined as model states, including endemic incidence during the rainy season, ubiquitous hyperendemicity during the ... | 2010 | 20018546 |
| presenilin/gamma-secretase cleaves cd46 in response to neisseria infection. | cd46 is a type i transmembrane protein with complement and t cell regulatory functions in human cells. cd46 has signaling and receptor properties in immune and nonimmune cells, many of which are dependent on the expression of cytoplasmic tail (cyt) isoforms cyt1 or cyt2. little is known about how cyt1 and cyt2 mediate cellular responses. we show that cd46-cyt1 and cd46-cyt2 are substrates for presenilin/gamma-secretase (ps/gammas), an endogenous protease complex that regulates many important sig ... | 2010 | 20018629 |
| cem-101, a novel fluoroketolide: antimicrobial activity against a diverse collection of gram-positive and gram-negative bacteria. | cem-101 is a novel fluoroketolide with reported high potency against diverse groups of gram-positive (micrococcus spp., viridans group streptococci, corynebacterium spp. listeria monocytogenes, clostridium spp., etc.) and gram-negative bacteria (neisseria gonorrhoeae, campylobacter jejuni, helicobacter pylori, bacteroides fragilis, shigella spp., etc.), including mycoplasma and ureaplasma, as well as bacteria commonly associated with community-acquired respiratory tract infections and skin and s ... | 2010 | 20022192 |
| mechanisms of meningeal invasion by a bacterial extracellular pathogen, the example of neisseria meningitidis. | the blood-cerebrospinal fluid (csf) barrier physiologically protects the meningeal spaces from bloodborne bacterial pathogens, due to the existence of specialized junctional interendothelial complexes. a few bacterial pathogens are able to reach the subarachnoidal space and cause bacterial meningitis in humans, a rare but dreadful disease. surprisingly, most of them are extracellular commensals of the nasopharynx (neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae) or of ... | 2010 | 20026234 |
| direct 16s rrna gene sequencing of polymicrobial culture-negative samples with analysis of mixed chromatograms. | two cases involving polymicrobial culture-negative samples were investigated by 16s rrna gene sequencing, with analysis of mixed chromatograms. fusobacterium necrophorum, prevotella intermedia and streptococcus constellatus were identified from pleural fluid in a patient with lemierre's syndrome and neisseria meningitidis and escherichia coli were identified from a petechia in a patient with meningococcal disease. | 2010 | 20035024 |
| randomized trial to assess immunogenicity and safety of haemophilus influenzae type b and neisseria meningitidis serogroups c and y-tetanus toxoid conjugate vaccine in infants. | study assessed the immunogenicity and safety of an investigational haemophilus influenzae type b-neisseria meningitidis serogroups c and y-tetanus toxoid conjugate vaccine (hibmency-tt) in infants. | 2010 | 20035207 |
| [hospital admissions for meningococcal infections in madrid, spain (1997-2005)]. | neisseria meningitidis infection causes an important morbidity and mortality in spain. our study aims to estimate the burden of hospital admissions for meningococcal infection in spain during a nine-year period (1997-2005) by analyzing the spanish hospital surveillance system. | 2010 | 20036397 |
| smoking status interacts with the association between mannose-binding lectin serum levels and gene polymorphism and the carriage of oropharyngeal bacteria. | mannose-binding lectin (mbl) role in the carriage of oropharyngeal bacteria is not known. we investigated the association of smoking, mbl2 polymorphisms, and mbl concentrations with oropharyngeal carriage of respiratory bacteria in young men. oropharyngeal specimens, mbl concentrations, and mbl2 gene polymorphisms were measured in 124 asthmatic and 394 nonasthmatic finnish military recruits. the carriage rates of s. pneumoniae (p = 0.002), n. meningitidis (p = 0.005), and beta-hemolytic streptoc ... | 2010 | 20038440 |
| meningococcal porin porb prevents cellular apoptosis in a toll-like receptor 2- and nf-kappab-independent manner. | meningococcal porin porb is an inhibitor of apoptosis induced via the intrinsic pathway in various cell types. this effect is attributed to prevention of mitochondrial depolarization and of subsequent release of proapoptotic mitochondrial factors. to determine whether apoptosis is globally inhibited by porb, we compared the intrinsic and extrinsic pathways in hela cells. interestingly, porb does not prevent extrinsic apoptosis induced by tumor necrosis factor alpha plus cycloheximide, suggesting ... | 2010 | 20028813 |
| spread of a chromosomal cefixime-resistant pena gene among different neisseria gonorrhoeae lineages. | in neisseria gonorrhoeae, the mosaic type of pena, which encodes penicillin-binding protein 2 (pbp 2), is associated with reduced susceptibility to oral cephalosporins. to investigate the relatedness of n. gonorrhoeae clinical isolates with reduced susceptibility, we sequenced the pena genes of 32 isolates. five different amino acid sequence types of pbp 2 were identified, but all seemed to be derivatives of pattern x of pbp 2 (pbp 2-x). however, multilocus sequence typing of the isolates showed ... | 2010 | 20028823 |
| cluster of serogroup w135 meningococci, southeastern florida, 2008-2009. | recently, 14 persons in southeastern florida were identified with neisseria meningitidis serogroup w135 invasive infections. all isolates tested had matching or near-matching pulsed-field gel electrophoresis patterns and belonged to the multilocus sequence type 11 clonal complex. the epidemiologic investigation suggested recent endemic transmission of this clonal complex in southeastern florida. | 2010 | 20031054 |
| safety and immunogenicity of an investigational quadrivalent meningococcal conjugate vaccine after one or two doses given to infants and toddlers. | with the emergence of multiple meningococcal serogroups in different geographic areas, broad vaccine protection from infancy is desirable. one hundred and seventy-five infants received either two doses of a meningococcal quadrivalent (a, c, w-135, y) conjugate vaccine (menacwy-crm) at 6 and 12 months, one dose of menacwy-crm at 12 months, or menc at 12 months and menacwy-crm at 18 months. bactericidal antibody titers using human complement were measured before and 1 month after each dose. inject ... | 2010 | 20033465 |
| clonal distribution of disease-associated and healthy carrier isolates of neisseria meningitidis between 1983 and 2005 in cuba. | in response to epidemic levels of serogroup b meningococcal disease in cuba during the 1980s, the va-mengoc-bc vaccine was developed and introduced into the national infant immunization program in 1991. since then the incidence of meningococcal disease in cuba has returned to the low levels recorded before the epidemic. a total of 420 neisseria meningitidis strains collected between 1983 and 2005 in cuba were analyzed by multilocus sequence typing (mlst). the set of strains comprised 167 isolate ... | 2010 | 20042619 |
| frequency of factor h-binding protein modular groups and susceptibility to cross-reactive bactericidal activity in invasive meningococcal isolates. | meningococcal factor h-binding protein (fhbp) is a promising vaccine candidate that elicits serum bactericidal antibodies in humans. based on sequence variability of the entire protein, fhbp has been divided into three variant groups or two sub-families. we recently reported that the fhbp architecture was modular, consisting of five variable segments, each encoded by genes from one of two lineages. based on combinations of segments from different lineages, all 70 known fhbp sequence variants cou ... | 2010 | 20044056 |
| neisseria gonorrhoeae survival during primary human cervical epithelial cell infection requires nitric oxide and is augmented by progesterone. | neisseria gonorrhoeae is an obligate human pathogen that causes gonorrhea. we have shown previously that complement receptor 3 and akt kinase play important roles in mediating cervical infection. at present, there are limited data to indicate how hormonally induced changes to the mucosal epithelia of the female genital tract mediate the course of gonococcal disease. hence, i have expanded upon previous work to investigate the interaction of gonococci with primary human cervical epithelial (pex) ... | 2010 | 20048043 |
| correlates of cervical mycoplasma genitalium and risk of preterm birth among peruvian women. | mycoplasma genitalium is associated with cervicitis and pelvic inflammatory disease in nonpregnant women. we investigated associations between cervical m genitalium, demographic and behavioral risk factors for sexually transmitted infection and preterm birth among low-income peruvian women. | 2010 | 20051932 |
| immunogenicity of haemophilus influenzae type b conjugate vaccines in korean infants: a meta-analysis. | a meta-analysis was performed on the immunogenicity of haemophilus influenzae type b (hib) conjugate vaccines after 2 (2 and 4 months) and 3 doses (2, 4, and 6 months) in korean infants. a database search of medline, koreamed, and korean medical database was done. the primary outcome measure was the proportion of infants with anti-polyribosylribitol phosphate (prp) concentrations > or =1.0 microg/ml. eight studies including eleven trials were retrieved. one trial reported on the diphtheria toxoi ... | 2010 | 20052353 |
| phenotypic and molecular characterization of invasive serogroup w135 neisseria meningitidis strains from 1990 to 2005 in brazil. | neisseria meningitidis serogroup w135 has been associated with global outbreaks since the 2000 hajj. considering that n. meningitidis serogroup w135 is the third most prevalent serogroup isolated in brazil in the last 10 years, and the possibility that the hajj-related n. meningitidis serogroup w135 clone has been causing disease in brazil, the present study characterized invasive n. meningitidis serogroup w135 isolates recovered in brazil from 1990 to 2005. | 2010 | 20056121 |
| meningococcal serogroup c polysaccharide specific memory b cells, directly enumerated by labeled polysaccharide, are not affected by age at vaccination. | the influence of age on the generation and persistence of specific memory b cells after vaccination with neisseria meningitidis type c polysaccharide (menc-ps) conjugate is unknown. menc-ps-specific b cells could be directly enumerated by fluorochrome-labeled menc-ps and flow cytometry in blood up to at least 4 years after vaccination, ranging from 0.01% to 0.78% of total b cells and did not correlate with age at vaccination. the percentage of menc-specific memory b cells out of total memory b c ... | 2010 | 20056181 |
| peritoneal dialysis-related peritonitis due to neisseria weaveri: the first case report. | 2010 | 20056994 | |
| chlamydia trachomatis causing neonatal conjunctivitis in a tertiary care center. | chlamydia trachomatis is considered a major aetiological agent of conjunctivitis in newborns. the objective of the present study was to determine the aetiology of neonatal conjunctivitis and clinico-epidemiological correlates of chlamydial ophthalmia neonatorum. fifty-eight newborns with signs and symptoms of conjunctivitis were studied. conjunctival specimens were subjected to gram staining, routine bacteriological culture, culture for neisseria gonorrhoeae and direct fluorescent antibody (dfa) ... | 2010 | 20061763 |
| overweight and obesity as risk factors for the asymptomatic carrier state of neisseria meningitidis among a paediatric population. | we analysed the asymptomatic carrier state of neisseria meningitidis in a sample of 339 children. we obtained data for the children's weight and height, in order to calculate the body mass index (bmi). the cutoff points defined by cole were employed in determining the bmi, and the population was divided into three groups: normal, overweight and obese. twenty carriers of n. meningitidis were identified. there was found to be a statistically significant trend to increased risk of being a carrier w ... | 2010 | 20063028 |
| comparative in vitro activities of nemonoxacin (tg-873870), a novel nonfluorinated quinolone, and other quinolones against clinical isolates. | the in vitro antibacterial activities of nemonoxacin (tg-873870), a novel nonfluorinated quinolone, against 770 clinical isolates were investigated. nemonoxacin (tested as its malate salt, tg-875649) showed better in vitro activity than ciprofloxacin and levofloxacin against different species of staphylococci, streptococci, and enterococci, neisseria gonorrhoeae, and haemophilus influenzae. the in vitro activity of tg-875649 was also comparable to or better than that of moxifloxacin against thes ... | 2010 | 20065058 |
| influence of serogroup b meningococcal vaccine antigens on growth and survival of the meningococcus in vitro and in ex vivo and in vivo models of infection. | a novel vaccine against serogroup b meningococcal disease - containing a combination of protein antigens identified by reverse vaccinology: fhbp fused to gna2091, gna2132 fused to gna1030, and nada - is currently in phase iii clinical trials. in order to determine the role of these antigens in the growth, survival and fitness of the meningococcus, we generated a mutant lacking the expression of all five protein antigens (5ko), a mutant lacking the three main antigens (fhbp, gna2132 and nada; 3ko ... | 2010 | 20067752 |
| travel-related cerebro-meningeal infections: the 8-year experience of a french infectious diseases unit. | the etiological spectrum of cerebro-meningeal infections (cmi) in travelers has never been specifically analyzed. | 2010 | 20074095 |
| identification of a novel transcriptional regulator involved in pilc1 regulation in neisseria meningitidis. | abstract type iv pili are crucial for the virulence of neisseria meningitidis. pilc proteins belong to the complex protein machinery required for pili biosynthesis. the expression of the pilc1 gene is known to be induced during host cell contact and to be tightly controlled through four promoters, two transcription factors and a two-component signal transduction system. by screening of an insertional-mutant library, we identified a novel regulatory protein, i.e. nma1805, involved in the pilc1 co ... | 2010 | 20074296 |
| journal club. a cell biologist connects her research to bacterial brain invasion. | 2010 | 20075880 | |
| gonococcal inflammation of paraurethral glands around the external urethral orifice in males: a commonly encountered disease? | 2010 | 20071443 | |
| immune response and one-year antibody persistence after a fourth dose of a novel haemophilus influenzae type b and neisseria meningitidis serogroups c and y-tetanus toxoid conjugate vaccine (hibmency) at 12 to 15 months of age. | a total of 236 infants received a fourth haemophilus influenzae type b-neisseria meningitidis serogroups c and y-tetanus toxoid conjugate vaccine (hibmency) dose at 12 to 15 months. one month later, the proportion with anti-prp antibody > or =1.0 microg/ml and bactericidal titers > or =1:8 to menc and meny was 98.9%, 96.9%, and 95.4%, respectively. one year later, anti-prp concentrations > or =0.15 microg/ml, and menc and meny bactericidal titers > or =1:8 persisted in 100%, 96.6%, and 83.8%, re ... | 2010 | 20072077 |
| correlates of unprotected sex with female sex workers among male clients in tijuana, mexico. | tijuana, situated adjacent to san diego, ca on the us-mexico border, is experiencing an emerging hiv epidemic, with prevalence among female sex workers (fsws) having risen in recent years from <1% to 6%. comparable data on fsws' clients are lacking. we explored correlates of unprotected sex with fsws among male clients in tijuana. | 2010 | 20081558 |
| [effectiveness of a polymerase chain reaction using seminested and multiplex strategy for the identification of the three main bacteria responsible for meningitis in burkina faso]. | a prospective study (from august 2006 to april 2007) was carried out with 214 cerebrospinal fluid samples with suspicion of bacterial meningitis. the aim of the study was to assess the effectiveness of the simultaneous detection of neisseria meningitidis, streptococcus sp. and haemophilus influenzae using seminested polymerase chain reaction strategy. among the 214 samples tested by both pcr and culture, the overall confirmation rate was 64% for pcr and 40.1% for culture (p = 2 x 10⁻⁶). taking c ... | 2010 | 20084483 |
| epidemiology of invasive meningococcal disease with decreased susceptibility to penicillin in ontario, canada, 2000 to 2006. | neisseria meningitidis has been relatively slow to acquire resistance to penicillin. we previously reported an increase in the incidence of invasive meningococcal disease (imd) strains with decreased susceptibility to penicillin (dsp) in ontario. our objectives were to evaluate trends in imd with dsp, to identify case-level predictors of imd with dsp, and to evaluate the relationship among dsp, bacterial phenotype, and the likelihood of a fatal outcome. all imd isolates received in ontario betwe ... | 2010 | 20086160 |
| antibiograms and randomly amplified polymorphic dna-polymerase chain reactions (rapd-pcr) as epidemiological markers of gonorrhea. | the development of antimicrobial resistance of neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. in this study, a total of 235 isolates of n. gonorrhoeae isolated from patients of bangrak hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. mutation (ser-91) in the quinolone resistance determining regions of gyra and random amplification of the pol ... | 2010 | 20087948 |
| direct validation of ngcgm3 ganglioside as a new target for cancer immunotherapy. | the target concept means not only an aberrant expression of a particular molecule in tumour tissues but also evidence of a clear therapeutic advantage, as a consequence of immune-intervention, in an antigen-positive relevant tumour model. since we reported the presence of ngcgm3 ganglioside in human breast tumours years ago and though phase i clinical trials of a ganglioside containing vaccine have been conducted, a definitive direct validation of this peculiar molecule as target for cancer immu ... | 2010 | 20088712 |
| optimizing protection against meningococcal disease. | neisseria meningitidis is a leading cause of bacterial meningitis and septicemia in the united states. vaccines directed against meningococcal disease must elicit high and persistent titers of bactericidal antibodies against prevalent meningococcal serogroups and be highly efficacious in preventing meningococcal infection. currently, 2 quadrivalent (a, c, w-135, y) vaccines-a polysaccharide meningococcal vaccine and a conjugate meningococcal vaccine-are licensed in the united states. neither is ... | 2010 | 20089551 |
| audit of routine rectal swabs for gonorrhoea culture in women. | the british association for sexual health and hiv guidelines on the diagnosis and treatment of gonorrhoea (gc) in adults recommends rectal screening only in special circumstances. our genitourinary medicine clinic undertakes rectal swabs for gonorrhoea culture in all women attending for sexually transmitted infection screening. we undertook this audit to determine the value of our practice. | 2010 | 20090002 |
| screening for asymptomatic chlamydia in women: how often would gonorrhoea be missed? | 2010 | 20090005 | |
| population dynamics of neisseria gonorrhoeae in shanghai, china: a comparative study. | gonorrhea is a major sexually transmitted disease (std) in many countries worldwide. the emergence of fluoroquinolone resistance has complicated efforts to control and treat this disease. we report the first study of the evolutionary processes acting on transmission dynamics of a resistant gonococcal population from shanghai, china. we compare these findings with our previous study of the evolution of a fluoroquinolone sensitive gonococcal population from baltimore, md. | 2010 | 20092631 |
| various pena mutations together with mtrr, porb and pona mutations in neisseria gonorrhoeae isolates with reduced susceptibility to cefixime or ceftriaxone. | to examine mutations within the pena, mtrr, porb, pona and pilq genes of neisseria gonorrhoeae to determine their contribution to cephalosporin resistance. | 2010 | 20093260 |
| structural basis for the recognition and cleavage of polysialic acid by the bacteriophage k1f tailspike protein endonf. | an alpha-2,8-linked polysialic acid (polysia) capsule confers immune tolerance to neuroinvasive, pathogenic prokaryotes such as escherichia coli k1 and neisseria meningitidis and supports host infection by means of molecular mimicry. bacteriophages of the k1 family, infecting e. coli k1, specifically recognize and degrade this polysia capsule utilizing tailspike endosialidases. while the crystal structure for the catalytic domain of the endosialidase of bacteriophage k1f (endonf) has been solved ... | 2010 | 20096705 |
| antimicrobial and antimalarial activity of cussonia species (araliaceae). | cussonia species are used in african traditional medicine mainly against pain, inflammation, gastro-intestinal problems, malaria and sexually transmitted diseases. | 2010 | 20298772 |
| 1h, 13c and 15n assignment of the c-terminal domain of gna2132 from neisseria meningitidis. | gna2132 (genome-derived neisseria antigen 2132) is a surface-exposed lipoprotein discovered by reverse vaccinology and expressed by genetically diverse neisseria meningitidis strains (pizza et al. 2000). the protein induces bactericidal antibodies against most strains of meningococccus and has been included in a multivalent recombinant vaccine against n. meningitidis serogroup b. structure determination of gna2132 is important for understanding the antigenic properties of the protein in view of ... | 2010 | 20300890 |
| type iv secretion machinery promotes ton-independent intracellular survival of neisseria gonorrhoeae within cervical epithelial cells. | survival of neisseria gonorrhoeae within host epithelial cells is expected to be important in the pathogenesis of gonococcal disease. we previously demonstrated that strain fa1090 derives iron from a host cell in a process that requires the ton complex and a putative tonb-dependent transporter, tdff. fa1090, however, lacks the gonococcal genetic island (ggi) that is present in the majority of strains. the ggi in strain ms11 has been partially characterized, and it encodes a type iv secretion sys ... | 2010 | 20308306 |
| home screening compared with clinic-based screening for sexually transmitted infections. | to estimate completion rate and acceptability of home screening for sexually transmitted infections (stis) compared with clinic-based screening in a prospective cohort study. | 2010 | 20308834 |
| triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection. | rapid and simple analysis for the multiple target pathogens is critical for patient management. ce-sscp analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields. the capability of separating ssdna molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram. in this study, we explored the potential of poly(ethyleneoxide)-pol ... | 2010 | 20309929 |
| community-acquired bacterial meningitis in alcoholic patients. | alcoholism is associated with susceptibility to infectious disease, particularly bacterial pneumonia. in the present study we described characteristics in alcoholic patients with bacterial meningitis and delineate the differences with findings in non-alcoholic adults with bacterial meningitis. | 2010 | 20161709 |
| a regulatory role for macrophage class a scavenger receptors in tlr4-mediated lps responses. | recognition of microbial components by tlr, key sensors of infection, leads to induction of inflammatory responses. we found that, in vivo, tlr4 engagement by lps induces up-regulation of the class a scavenger receptors (sr) macrophage receptor with a collagenous structure (marco) and sr-a, which occurs, at least in the case of marco, via both myd88-dependent and -independent pathways. when challenging mice with a low dose of lps followed by a high dose, class a sr-deficient mice showed a higher ... | 2010 | 20162551 |
| skin culture: another diagnostic tool in chronic meningococcaemia. | 2010 | 20331447 | |
| acute bacterial meningitis in adults: a hospital based study in yemen. | acute bacterial meningitis is an important cause of mortality and morbidity with high rates of long-term neurological sequelae. to determine the clinical presentation, complications, and outcome of acute meningitis in yemen, a retrospective study in patients 15 years or older with acute bacterial meningitis who were admitted into al-thawra teaching hospital in sana'a from january 2006 to december 2007 was carried out. there were 121 patients with acute bacterial meningitis. lumbar puncture was p ... | 2010 | 20332577 |
| nucleic acid amplification tests for diagnosis of neisseria gonorrhoeae and chlamydia trachomatis rectal infections. | it is uncertain which methods for the diagnosis of rectal gonococcal and chlamydial infection are optimal. this study evaluated the performance of culture and nucleic acid amplification tests (naats) for rectal chlamydial and gonococcal diagnosis. from july 2003 until february 2007, 441 rectal test sets were collected from individuals attending a sexually transmitted disease clinic and three hiv clinics who gave a history of anal intercourse or were women at high risk for neisseria gonorrhoeae o ... | 2010 | 20335410 |
| investigating the candidacy of lps-based glycoconjugates to prevent invasive meningococcal disease: chemical strategies to prepare glycoconjugates with good carbohydrate loading. | in previous studies protective antibodies that could facilitate bactericidal killing of neisseria meningitidis (nm) serogroup b strains were derived from immunisation with glycoconjugates prepared from o-deacylated lipopolysaccharide (lps-oh) via direct reductive amination between the reducing end of the oligosaccharide molecule, created by treatment with alkaline phosphatase, and amino functionalities on the crm(197) carrier protein. these glycoconjugates proved difficult to prepare because the ... | 2010 | 20340043 |
| conjugative plasmids of neisseria gonorrhoeae. | many clinical isolates of the human pathogen neisseria gonorrhoeae contain conjugative plasmids. the host range of these plasmids is limited to neisseria species, but presence of a tetracycline (tetm) determinant inserted in several of these plasmids is an important cause of the rapid spread of tetracycline resistance. previously plasmids with different backbones (dutch and american type backbones) and with and without different tetm determinants (dutch and american type tetm determinants) have ... | 2010 | 20376355 |
| biofilm formation by the human pathogen neisseria meningitidis. | the past decade has seen an increasing interest in biofilm formation by neisseria meningitidis, a human facultative pathogen causing life-threatening childhood disease commencing from asymptomatic nasopharyngeal colonization. studying the biology of in vitro biofilm formation improves the understanding of inter-bacterial processes in asymptomatic carriage, of bacterial aggregate formation on host cells, and of meningococcal population biology. this paper reviews publications referring to meningo ... | 2010 | 20376486 |
| identification of a novel transcriptional regulator involved in pilc1 regulation in neisseria meningitidis. | type iv pili are crucial for the virulence of neisseria meningitidis. pilc proteins belong to the complex protein machinery required for pili biosynthesis. the expression of the pilc1 gene is known to be induced during host cell contact and to be tightly controlled through four promoters, two transcription factors and a two-component signal transduction system. by screening of an insertional-mutant library, we identified a novel regulatory protein, i.e. nma1805, involved in the pilc1 complex reg ... | 2010 | 20377643 |
| 'neisseria skkuensis' sp. nov., isolated from the blood of a diabetic patient with a foot ulcer. | a gram-negative bacterium was isolated from the blood of a patient with diabetes mellitus. however, it could not be identified by conventional microbiological methods, and so was characterized by phenotypic and genotypic analyses. 16s rrna gene sequence analysis revealed that the strain belonged to the genus neisseria. based on the phenotypic and genotypic characteristics, we propose that strain smc-a9199(t) (=kctc 22696(t)=jcm 16127(t)) should be classified as a novel species, 'neisseria skkuen ... | 2010 | 20378725 |
| gonococcal naats: what is the current state of play in england and wales? | the aim of this study was to determine how widespread the use is of dual nucleic acid amplification tests (naats) for the diagnosis of both chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) in england and wales. a structured telephone questionnaire was used to collect information on the method of dual testing used by laboratories, the patient groups tested, the types of specimens obtained and the use of gc culture. of the 108 laboratories participating, 29% performed dual ct and gc naat ... | 2010 | 20378894 |
| pilot study of the utility and acceptability of tampon sampling for the diagnosis of neisseria gonorrhoeae and chlamydia trachomatis infections by duplex realtime polymerase chain reaction in united kingdom sex workers. | we aimed to evaluate the acceptability of self-collected tampon samples for the screening of female sex workers for sexually transmitted infections. we recruited 65 sex workers, and 63 agreed to provide tampon samples. the tampon samples were processed by realtime polymerase chain reaction (pcr) targeting neisseria gonorrhoeae and chlamydia trachomatis. urethral and endocervical swabs were also obtained from 61 of 63 participants and tested using culture (n. gonorrhoeae) and the bd probetec stra ... | 2010 | 20378902 |
| a pilot study on the prevalence of sexually transmitted infections among clients of brothel-based female sex workers in jessore, bangladesh. | the prevalence of bacterial sexually transmitted infections among clients of female sex workers (fsws) was studied. a total of 156 condoms were collected from clients of 13 sex workers during three consecutive days. condoms used by clients of fsws were collected and dna was extracted from the seminal fluid and used for the diagnosis of neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis by polymerase chain reaction. the prevalence of n. gonorrhoeae, c. trachomatis and t. vagin ... | 2010 | 20378907 |
| virulence determinants involved in differential host niche adaptation of neisseria meningitidis and neisseria gonorrhoeae. | neisseria meningitidis and neisseria gonorrhoeae are the only pathogenic species of the genus neisseria. although these two species are closely related, they specialized on survival in completely different environments within the human host-the nasopharynx in the case of n. meningitidis versus the urogenital tract in the case of n. gonorrhoeae. the genetic background of these differences has not yet been determined. here, we present a comparison of all characterized transcriptional regulators in ... | 2010 | 20379743 |
| lactobacillus jensenii surface-associated proteins inhibit neisseria gonorrhoeae adherence to epithelial cells. | high numbers of lactobacilli in the vaginal tract have been correlated with a decreased risk of infection by the sexually transmitted pathogen neisseria gonorrhoeae. we have previously shown that lactobacillus jensenii, one of the most prevalent microorganisms in the healthy human vaginal tract, can inhibit gonococcal adherence to epithelial cells in culture. here we examined the role of the epithelial cells and the components of l. jensenii involved in the inhibition of gonococcal adherence. l. ... | 2010 | 20385752 |
| [isolation and determination of activity of iga1 protease from neisseria meningitidis]. | a method of the isolation and purification of igal protease from a culture of neisseria meningitidis serogroup a has been developed. three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and sediment obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. the purity of iga1 protease was determined by sds-page. an immunoenzyme assay for determining the iga1 protease activity has been devised. ... | 2010 | 20386581 |
| reduced adamts13 in children with severe meningococcal sepsis is associated with severity and outcome. | multiple organ failure is a common feature of pediatric meningococcal sepsis and is associated with an imbalance of coagulation and fibrinolysis. this is partly due to an increased secretion of prothrombotic ultra-large von willebrand factor (vwf) as the result of vascular endothelial damage. another factor that may contribute is adamts13, which converts vwf into smaller, less active, vwf multimers and thus influences vwf activity in plasma. we investigated the role of adamts13 and vwf in the se ... | 2010 | 20390223 |
| spontaneous resolution of extragenital chlamydial and gonococcal infections prior to therapy. | 2010 | 20393382 | |
| the russian gonococcal antimicrobial susceptibility programme (ru-gasp)--national resistance prevalence in 2007 and 2008, and trends during 2005-2008. | antimicrobial resistance (amr) in neisseria gonorrhoeae is a major problem worldwide. in the former soviet countries including russia, the knowledge regarding amr has been highly limited. however, in 2004 the russian gonococcal antimicrobial susceptibility programme (ru-gasp) was initiated. the aims of this study were to examine and describe the prevalence of n. gonorrhoeae amr in 2007 and 2008 in russia, and reveal trends in the period from 2005 to 2008. gonococcal isolates (660 in 2007 and 900 ... | 2010 | 20394716 |
| [recurrent fever and skin eruption revealing chronic meningococcemia]. | chronic meningococcemia is an unusual clinical presentation within the spectrum of infections due to neisseria meningitidis. | 2010 | 20395022 |
| lack of pneumococcal carriage in the hospitalised elderly. | there have been few surveys of streptococcus pneumoniae and neisseria meningitidis carriage in sick or frail elderly people who, with the very young, comprise the group who are at highest risk for pneumococcal disease. we studied pneumococcal carriage among participants in a pneumococcal immunisation study in the frail elderly. | 2010 | 20398618 |
| dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis. | to dissect the in vivo responses to lipopolysaccharide compared with nonlipopolysaccharide structures of whole meningococci. | 2010 | 20400898 |
| knowledge of interim recommendations and use of hib vaccine during vaccine shortages. | the goals were to determine among pediatricians and family physicians (1) knowledge of interim recommendations regarding haemophilus influenzae type b (hib) vaccine administration, (2) current practices, and (3) factors associated with nonadherence. | 2010 | 20403935 |
| generation of human ceacam1 transgenic mice and binding of neisseria opa protein to their neutrophils. | human ceacam1 is a cell-cell adhesion molecule with multiple functions including insulin clearance in the liver, vasculogenesis in endothelial cells, lumen formation in the mammary gland, and binding of certain human pathogens. | 2010 | 20404914 |
| ceacam1 recognition by bacterial pathogens is species-specific. | carcinoembryonic antigen-related cell adhesion molecule 1 (ceacam1), an immunoglobulin (ig)-related glycoprotein, serves as cellular receptor for a variety of gram-negative bacterial pathogens associated with the human mucosa. in particular, neisseria gonorrhoeae, n. meningitidis, moraxella catarrhalis, and haemophilus influenzae possess well-characterized ceacam1-binding adhesins. ceacam1 is typically involved in cell-cell attachment, epithelial differentiation, neovascularisation and regulatio ... | 2010 | 20406467 |
| epidemiology of meningococcal disease in latin america: current situation and opportunities for prevention. | meningococcal disease continues to be a serious public health concern, being associated with high morbidity and mortality rates in many countries from latin america. in addition to discussing recent changes in the epidemiology of meningococcal disease in the region, we also analyse the development and potential impact of new vaccines on the prevention of meningococcal disease. | 2010 | 20406604 |
| fatal dog bite in the absence of significant trauma: capnocytophaga canimorsus infection and unexpected death. | sudden and unexpected nontraumatic death in individuals with asplenia or hyposplenia is usually due to fulminant bacterial sepsis, most often involving streptococcus pneumoniae, neisseria meningitidis, and hemophilus influenzae. we report a case of a previously well 40-year-old man who died 5 hours after hospital admission. at autopsy waterhouse-friderichsen syndrome was identified and capnocytophaga canimorsus was subsequently isolated on antemortem blood cultures. infection of humans with this ... | 2010 | 20407357 |
| effect of atmospheric pressure non-equilibrium plasmas on neisseria gonorrhoeae. | in this study, the sterilizing effect of atmospheric pressure nonequilibrium plasmas (apnps) on neisseria gonorrhoeae (n. gonorrhoeae) was preliminarily examined and the possible mechanisms were explored. n. gonorrhoeae fa1090, fa19 and ms11 were treated by apnps and their survival rate was analyzed by using cfus counting and structurally studied by laser scanning confocal microscopy. the morphological changes of bacterial cell membrane and wall were studied under tem. our results showed that ap ... | 2010 | 20407879 |
| neisseria lactamica arthritis and septicemia complicating myeloma. | 2010 | 20410341 | |
| advances in the development of vaccines against neisseria meningitidis. | 2010 | 20410516 | |
| bacteraemia in children in iceland 1994-2005. | to investigate the aetiology of bacteraemia in children in iceland, the antibiotic resistance and possible preventive measures. | 2010 | 20412104 |
| genetic and structural characterization of l11 lipooligosaccharide from neisseria meningitidis serogroup a strains. | the lipooligosaccharide (los) of immunotype l11 is unique within serogroup a meningococci. in order to resolve its molecular structure, we conducted los genotyping by pcr analysis of genes responsible for alpha-chain sugar addition (lgta, -b, -c, -e, -h, and -f) and inner core substituents (lgtg, lpt-3, and lpt-6). for this study, we selected seven strains belonging to subgroup iii, a major clonal complex responsible for meningococcal meningitis epidemics in africa. in addition, we sequenced the ... | 2010 | 20421293 |
| nalp-mediated proteolytic release of lactoferrin-binding protein b from the meningococcal cell surface. | bacteria have developed several mechanisms for iron uptake during colonization of mammalian hosts, where the availability of free iron is limiting for growth. neisseria meningitidis expresses under iron-limiting conditions a receptor complex consisting of the lactoferrin-binding proteins a (lbpa) and lbpb to acquire iron from lactoferrin, which is abundantly present on the mucosal surfaces of the human nasopharynx. lbpa is an integral outer membrane-embedded iron transporter, whereas lbpb is a c ... | 2010 | 20421383 |
| identification of tem-135 beta-lactamase in penicillinase-producing neisseria gonorrhoeae strains in japan. | ten penicillinase-producing neisseria gonorrhoeae (ppng) strains isolated from 2000 to 2008 were characterized by multilocus sequence typing, multiantigen sequence typing, and plasmid typing. sequence analysis showed that 8 strains contained a tem-1 beta-lactamase gene. however, two other genetically distinct ppng strains, isolated in 2004 and 2008, each contained a tem-135 beta-lactamase on different plasmids, a toronto/rio type r plasmid and an asia type r plasmid, suggesting independent origi ... | 2010 | 20421400 |
| increasing hiv infectivity by neisseria gonorrhea: role of host antiapoptotic proteins in enhanced transmission. | 2010 | 20421747 | |
| comparison of dot-elisa and standard elisa for detection of neisseria meningitidis outer membrane complex-specific antibodies. | dot-elisa using the outer membrane complex antigens of neisseria meningitidis as a target was standardized for rapid detection of meningococcal-specific antibodies in human serum. we investigated the level of meningococcal-specific igg, iga, and igm in serum using dot-elisa with outer membrane antigens prepared from neisseria meningitidis serotype b:4.19:p1.15,3,7,9 (a strain isolated from a brazilian epidemic). the dot-elisa is based on the same principles as the standard elisa and is useful fo ... | 2010 | 20428652 |
| "ophthalmia venerea": a dreadful complication of fluoroquinolone-resistant neisseria gonorrhoeae. | infections caused by quinolone-resistant neisseria gonorrhoeae are increasing worldwide. although mostly mild and uncomplicated, serious infections causing severe morbidity are occasionally observed. we report 2 cases of sexually transmitted keratoconjunctivitis in adults resulting in severe visual disturbance. | 2010 | 20429088 |
| role of hfq in iron-dependent and -independent gene regulation in neisseria meningitidis. | in neisseria meningitidis, iron-responsive gene regulation is mediated primarily by the ferric uptake regulator (fur) protein. when complexed with iron, fur represses gene expression by preventing transcription initiation. fur can also indirectly activate gene expression via the repression of regulatory small rnas (srna). one such fur- and iron-regulated srna, nrrf, was previously identified in n. meningitidis and shown to repress expression of the sdha and sdhc genes encoding subunits of the su ... | 2010 | 20430815 |
| phase i/ii, open-label trial of safety and immunogenicity of meningococcal (groups a, c, y, and w-135) polysaccharide diphtheria toxoid conjugate vaccine in human immunodeficiency virus-infected adolescents. | quadrivalent meningococcal polysaccharide conjugate vaccine (mcv4) is routinely recommended for healthy youth in the united states, but there are no data about its use in hiv-infected people. | 2010 | 20431379 |
| culture of non-genital sites increases the detection of gonorrhea in women. | although gonorrhea may infect the cervix, rectum, or pharynx of women, culturing non-cervical sites is rare outside of sexually transmitted disease (std) clinics. this study aims to compare rectal and pharyngeal gonorrhea prevalence in adolescent and adult women and to calculate the percentage of cases that would be missed with cervical culture alone. | 2010 | 20434928 |
| detection of neisseria gonorrhoeae from vaginal swabs of ewin, rajaii shahr, karaj and varamin female prisoners by pcr and culture methods. | isolation of n. gonorrhoeae by culture method is currently the gold standard for the definitive diagnosis of gonorrhoea. however, pcr techniques are being used more frequently as sensitivity and specificity of the newer tests are improved. in this study, 500 vaginal swabs from ewin, rajaii shahr, karaj and varamin female prisoners were used for detection of n. gonorrhoae by culture and pcr techniques. five hundred vaginal swabs from ewin, rajaii shahr, karaj and varamin female prisoners were cul ... | 2010 | 20437689 |
| systematic functional analysis reveals that a set of seven genes is involved in fine-tuning of the multiple functions mediated by type iv pili in neisseria meningitidis. | type iv pili (tfp), which mediate multiple phenotypes ranging from adhesion to motility, are one of the most widespread virulence factors in bacteria. however, the molecular mechanisms of tfp biogenesis and associated functions remain poorly understood. one of the underlying reasons is that the roles played by the numerous genes involved in tfp biology are unclear because corresponding mutants have been studied on a case-by-case basis, in different species, and using different assays, often gene ... | 2010 | 20439474 |
| the structure of neisseria meningitidis lipid a determines outcome in experimental meningococcal disease. | lipopolysaccharide (lps), a major component of the meningococcal outer membrane, is sensed by the host through activation of toll-like receptor 4 (tlr4). recently, we demonstrated that a surprisingly large fraction of neisseria meningitidis disease isolates are lipid a mutants, due to inactivating mutations in the lpxl1 gene. the lpxl1 mutants activate human tlr4 much less efficiently than wild-type bacteria, which may be advantageous by allowing them to escape from the innate immune system. her ... | 2010 | 20439476 |
| polyamines can increase resistance of neisseria gonorrhoeae to mediators of the innate human host defense. | polyamines are biogenic polycationic molecules involved in key cellular functions. extracellular polyamines found in bodily fluids or laboratory media can be imported by bacteria or bind to negatively charged bacterial surface structures, where they can impair binding of antimicrobials. we hypothesized that the presence of polyamines in fluids that bathe urogenital mucosal surfaces could alter the susceptibility of the sexually transmitted strict human pathogen neisseria gonorrhoeae to mediators ... | 2010 | 20439477 |