Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| genetic analysis of chromosomal operons involved in degradation of aromatic hydrocarbons in pseudomonas putida tmb. | the catabolic pathway for the degradation of aromatic hydrocarbons encoded by pseudomonas putida tmb differs from the tol plasmid-encoded pathway as far as regulation of the upper pathway is concerned. we found, by analyzing tn5-induced mutants and by southern blot hybridization with appropriate probes derived from the tol plasmid pww0, that the catabolic genes of strain tmb were located on the bacterial chromosome and not on the 84-kb plasmid harbored by this strain. the catabolic genes of tmb ... | 1990 | 2172213 |
| transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. | a simple procedure for cloning and stable insertion of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition features of tn10 and tn5; (ii) the resistances to the herbicide bialaphos, to mercuric salts and organomercurial compounds, and to arsenite, and (iii) the suicide delivery properties of the r6k-based plasmid pgp704. the resulting constructions contained unique noti or sfii sites internal to either the tn10 o ... | 1990 | 2172216 |
| isolation of high frequency of recombination donors from tn5 chromosomal mutants of pseudomonas putida ppn and recalibration of the genetic map. | a tn5 loaded derivative of the incp-10 plasmid r91-5 (pmo75) was used as a suicide vector to generate random chromosomal insertion mutations in pseudomonas putida ppn. reintroduction of pmo75 into such mutants resulted in integration of the plasmid at the site of tn5 insertion, giving rise to two classes of high frequency of donors recombination (hfr) donors, transferring chromosome at high frequency (greater than 10(-1) per donor cell) in opposite directions. consequently, tn5 induced auxotroph ... | 1990 | 2174392 |
| upstream regulatory sequence for transcriptional activator xylr in the first operon of xylene metabolism on the tol plasmid. | transcription of the first operon coding for m-xylene-degrading enzymes on the tol plasmid of pseudomonas putida is activated by the xylr gene product in the presence of m-xylene. the operon has the consensus sequence of the ntr/nif promoters at -24 and -12 regions, and the transcription is dependent on an rna polymerase containing a sigma factor ntra (rpon or sigma 54). deletion derivatives of the upstream sequence of the operon promoter were made in vitro and connected with the xyle gene on a ... | 1990 | 2174974 |
| design of an enzymatic hybrid system: a useful strategy for the biosynthesis of benzylpenicillin in vitro. | a hybrid (prokaryotic-eukaryotic) enzyme system leading to the production of benzylpenicillin has been developed. in vitro synthesis of penicillin g was achieved by incubating 6-aminopenicillanic acid, coa, phenylacetic acid, homogeneously pure phenylacetyl-coa ligase (pa-coa ligase) from pseudomonas putida and acyl-coa:6-apa acyltransferase (at) from penicillium chrysogenum. benzylpenicillin was also obtained when at was coupled with pa-coa ligase and isopenicillin n-synthetase (ipns). this is ... | 1990 | 2178138 |
| putidaredoxin reductase and putidaredoxin. cloning, sequence determination, and heterologous expression of the proteins. | the oxidation of camphor by cytochrome p-450cam requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from nadh to p-450 for oxygen activation. a 2.2-kilobase pair bamhi-stui fragment from whole cell dna of camphor-grown pseudomonas putida has been cloned and sequenced. translation of the sequence revealed two open reading frames that could code for putidaredoxin reductase and putidaredoxin. in the c ... | 1990 | 2180940 |
| the meta cleavage operon of tol degradative plasmid pww0 comprises 13 genes. | the meta-cleavage operon of tol plasmid pww0 of pseudomonas putida encodes a set of enzymes which transform benzoate/toluates to krebs cycle intermediates via extradiol (meta-) cleavage of (methyl)catechol. the genetic organization of the operon was characterized by cloning of the meta-cleavage genes into an expression vector and identification of their products in escherichia coli maxicells. this analysis showed that the meta-cleavage operon contains 13 genes whose order and products (in kiloda ... | 1990 | 2183008 |
| characterization of the multiple catalytic activities of tartrate dehydrogenase. | tartrate dehydrogenase (tdh) has been purified to apparent homogeneity from pseudomonas putida and has been demonstrated to catalyze three different nad(+)-dependent reactions. tdh catalyzes the oxidation of (+)-tartrate to form oxaloglycolate and the oxidative decarboxylation of d-malate to form pyruvate and co2. d-glycerate and co2 are formed from meso-tartrate in a reaction that is formally a decarboxylation with no net oxidation or reduction. the steady-state kinetics of the first two reacti ... | 1990 | 2184888 |
| organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pjp4. | growth of alcaligenes eutrophus jmp134 on 2,4-dichlorophenoxyacetate requires a 2,4-dichlorphenol hydroxylase encoded by gene tfdb. catabolism of either 2,4-dichlorophenoxyacetate or 3-chlorobenzoate involves enzymes encoded by the chlorocatechol oxidative operon consisting of tfdcdef, which converts 3-chloro- and 3,5-dichlorocatechol to maleylacetate and chloromaleylacetate, respectively. transposon mutagenesis has localized tfdb and tfdcdef to ecori fragment b of plasmid pjp4 (r. h. don, a. j. ... | 1990 | 2185214 |
| a family of positive regulators related to the pseudomonas putida tol plasmid xyls and the escherichia coli arac activators. | the xyls family consists of a least 8 different transcriptional regulators. six of these proteins are positive regulators for the catabolism of carbon sources (benzoate and sugars) in escherichia coli, pseudomonas putida and erwinia carotovora, and two of them are involved in pathogenesis in escherichia coli and yersinia enterocolitica. based on protein alignments, the members of this family exhibit a long stretch of homology at the c-terminal end. the regulators involved in the catabolism of ca ... | 1990 | 2186376 |
| electroporation and expression of plasmid pbr322 in klebsiella aerogenes nctc 418 and plasmid prk2501 in pseudomonas putida cym 318. | klebsiella aerogenes nctc 418 and pseudomonas putida cym 318 were transformed via high-voltage electroporation with plasmids pbr322 and prk2501, respectively. the number of transformants obtained was dependent on the applied voltage, capacitance, and cell recovery procedure. for example, 7.87 x 10(4) transformants/micrograms dna were obtained at 2500 v, 25 muf when k. aerogenes cells were electroporated with pbr322 dna. a lower voltage (1500) and capacitance (3 muf) yielded 2.4 x 10(3) transform ... | 1990 | 2187074 |
| microcosm for assessing survival of genetically engineered microorganisms in aquatic environments. | laboratory-contained microcosms are important for studying the fate and survival of genetically engineered microorganisms. in this study, we describe a simple aquatic microcosm that utilizes survival chambers in a flowthrough or static renewal system. the model was used to study the survival of genetically engineered and wild-type strains of escherichia coli and pseudomonas putida in the lake water environment. temperature-dependent studies indicated that the genetically engineered microorganism ... | 1990 | 2187407 |
| mutations leading to constitutive expression from the tol plasmid meta-cleavage pathway operon are located at the c-terminal end of the positive regulator protein xyls. | the xyls protein is the positive activator of the tol plasmid meta-cleavage pathway operon for the metabolism of alkylbenzoates in pseudomonas putida. the regulator stimulates transcription from the tol meta pathway operon promoter (pm) when activated by benzoate effectors or in the absence of effectors when overproduced. xyls mutant alleles that encode regulators which constitutively mediate expression from pm were isolated and characterized. the mutant proteins all exhibit single amino acid su ... | 1990 | 2193914 |
| cloning and expression of the ponb gene, encoding penicillin-binding protein 1b of escherichia coli, in heterologous systems. | a fragment from the ponb region of the escherichia coli chromosome comprising the promoterless sequence encoding penicillin-binding protein 1b (pbp 1b) has been cloned in a broad-host-range expression vector under the control of the kanamycin resistance gene promoter present in the vector. the hybrid plasmid (pjp3) was used to transform appropriate strains of salmonella typhimurium, pseudomonas putida, and pseudomonas aeruginosa. in all instances, the coding sequence was expressed in the heterol ... | 1990 | 2198260 |
| evaluation of autoscan-w/a automated microbiology system for the identification of non-glucose-fermenting gram-negative bacilli. | we evaluated the ability of the autoscan-w/a (microscan division, baxter healthcare corporation, west sacramento, calif.), in conjunction with the dried colorimetric neg id type 2 panel (dcp) and new rapid fluorometric neg id panel (rfp), to identify non-glucose-fermenting gram-negative bacilli by challenging the system with 310 previously identified reference strains. of these 310 isolates, 286 organisms were in the dcp data base and 269 were in the rfp data base. use of the dcp panels resulted ... | 1990 | 2199522 |
| cloning and sequence analysis of the genes encoding the alpha and beta subunits of the e1 component of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus. | a 4175-bp ecori fragment of dna that encodes the alpha and beta chains of the pyruvate dehydrogenase (lipoamide) component (e1) of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus has been cloned in escherichia coli. its nucleotide sequence was determined. open reading frames (pdha, pdhb) corresponding to the e1 alpha subunit (368 amino acids, mr 41,312, without the initiating methionine residue) and e1 beta subunit (324 amino acids, mr 35,306, without the initiating ... | 1990 | 2200674 |
| purification and characterisation of tol plasmid-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase of pseudomonas putida. | benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase, two enzymes of the xylene degradative pathway encoded by the plasmid tol of a gram-negative bacterium pseudomonas putida, were purified and characterized. benzyl alcohol dehydrogenase catalyses the oxidation of benzyl alcohol to benzaldehyde with the concomitant reduction of nad+; the reaction is reversible. benzaldehyde dehydrogenase catalyses the oxidation of benzaldehyde to benzoic acid with the concomitant reduction of nad+; the re ... | 1990 | 2202600 |
| nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of pseudomonas putida. | the hutc gene of pseudomonas putida encodes a repressor which, in combination with the inducer urocanate, regulates expression of the five structural genes necessary for conversion of histidine to glutamate, ammonia, and formate. the nucleotide sequence of the hutc region was determined and found to contain two open reading frames which overlapped by one nucleotide. the first open reading frame (orf1) appeared to encode a 27,648-dalton protein of 248 amino acids whose sequence strongly resembled ... | 1990 | 2203753 |
| nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of klebsiella aerogenes. | the hutc gene of klebsiella aerogenes encodes a repressor that regulates expression of the histidine utilization (hut) operons. the dna sequence of a region known to contain hutc was determined and shown to contain two long rightward-reading open reading frames (orfs). one of these orfs was identified as the 3' portion of the hutg gene. the other orf was the hutc gene. the repressor predicted from the hutc sequence contained a helix-turn-helix motif strongly similar to that seen in other dna-bin ... | 1990 | 2203754 |
| sequence and analysis of the rpon sigma factor gene of rhizobium sp. strain ngr234, a primary coregulator of symbiosis. | we report the nucleotide sequence of the rpon gene from broad-host-range rhizobium sp. strain ngr234 and analyze the encoded rpon protein, a sigma factor. comparative analysis of the deduced amino acid sequence of rpon from ngr234 with sequences from other gram-negative bacteria identified a perfectly conserved rpon box unique to rpon sigma factors. symbiotic regulatory phenotypes were defined for a site-directed internal deletion within the coding sequence of the rpon gene of rhizobium strain n ... | 1990 | 2211497 |
| transcriptional analysis of the promoter region of the pseudomonas putida branched-chain keto acid dehydrogenase operon. | branched-chain keto acid dehydrogenase is a multienzyme complex produced by pseudomonas putida when it is grown in a minimal medium containing branched-chain amino acids. a 1.87-kilobase (kb) dna fragment was cloned and sequenced which contained 0.24 kb of the e1 alpha structural gene and 1.6 kb of upstream dna. there were 854 base pairs (bp) of noncoding dna upstream of bkda1, the first gene of the bkd operon, and 592 bp between the transcriptional and translational starts. the g + c content of ... | 1990 | 2211503 |
| mandelate racemase and muconate lactonizing enzyme are mechanistically distinct and structurally homologous. | mandelate racemase (mr) and muconate lactonizing enzyme (mle) catalyse separate and mechanistically distinct reactions necessary for the catabolism of aromatic acids by pseudomonas putida. the x-ray crystal structure of mr, solved at 2.5 a resolution, reveals that the secondary, tertiary and quaternary structures of mr and mle are remarkably similar; also, mr and mle are about 26% identical in primary structure. however, mr has no detectable mle activity and vice versa. thus, mr and mle constitu ... | 1990 | 2215699 |
| functional modification of an arginine residue on salicylate hydroxylase. | salicylate hydroxylase from pseudomonas putida (ec 1.14.13.1, salicylate, nadh:oxygen oxidoreductase) is an fad-containing monooxygenase, which catalyzes decarboxylative hydroxylation of salicylate to produce catechol in the presence of nadh and o2. by chemical treatment of the enzyme with dicarbonyl reagents, such as glyoxal, the original oxygenase activity was converted to the salicylate-dependent nadh-dehydrogenase activity with free fad as electron acceptor. one of twenty arginine residues o ... | 1990 | 2223838 |
| different types of formaldehyde-oxidizing dehydrogenases in nocardia species 239: purification and characterization of an nad-dependent aldehyde dehydrogenase. | three different dehydrogenases able to oxidize formaldehyde were found in the gram-positive methylotroph, nocardia sp. 239: an nad-dependent aldehyde dehydrogenase (na-adh), and nad- and factor-dependent formaldehyde dehydrogenase (fd-fdh), and a dye-linked aldehyde dehydrogenase (dl-adh). the ratio of the activities observed for the two nad-linked enzymes varied with growth conditions: batch-wise grown cells had nearly the same activities for both enzymes; in fed batch-wise grown cells (methano ... | 1990 | 2241149 |
| [studies on respiratory infections in primary care clinic (iii). distribution of bacteria isolated from patients with respiratory infections visiting 21 private clinics in the tohoku district of japan]. | the bacteriology of the isolates from the throat swab and the sputum respectively of 2,539 patients with respiratory infections visiting 21 private clinics in tohoku district of japan during the period from january to april in 1989 was documented. of the 2,539 patients, 1,694 had an acute upper respiratory infection, 609 had acute bronchitis, 46 had acute pneumonia, 84 had acute exacerbation of chronic respiratory infections and 106 had respiratory infections without diagnosis registered. 1887 ( ... | 1990 | 2243193 |
| nucleotide sequence and expression of the isoamylase gene from an isoamylase-hyperproducing mutant, pseudomonas amyloderamosa jd210. | the isoamylase gene (iso) of pseudomonas amyloderamosa jd210, an isoamylase-hyperproducing mutant, was cloned in an isoamylase-deficient and transformable mutant strain k31. by deletion analysis, the iso gene was found to be located within a 3.3 kilobases bamhi fragment. its nucleotide sequence contained an open reading frame of 2328 nucleotides (776 amino acids) encoding a secreted isoamylase precursor. the iso gene fragment was inserted into plasmids pkt230 and pbr 322 in opposite orientations ... | 1990 | 2248978 |
| [the preparation and properties of catechol-1,2-dioxygenase from pseudomonas putida]. | catechol-1,2-dioxygenase (ec 1.13.11.1) catalyzes the degradation of catechol to cis, cis-muconic acid. the biochemical properties of catechol-1,2-dioxygenase from pseudomonas putida 84103 were investigated. the optimum ph and temperature is 7.5-8.0 and 25-30 degrees c, respectively. cu2+, zn2+ inhibit the enzyme activity. the paper chromatograph and uv absorption spectrum of enzymatic reaction product are accordance with those of the standard muconic acid. | 1990 | 2251833 |
| growth-phase-dependent expression of the pseudomonas putida tol plasmid pww0 catabolic genes. | pseudomonas putida tol plasmid pww0 catabolic genes are clustered into two operons. the first, the upper operon, is controlled by the xylr regulatory gene, whereas the second, the meta operon, is controlled by the xyls regulatory gene. the xyls gene itself is subjected to control by xylr. in this study, we show that the tol catabolic operons were poorly induced in cells growing at the early-exponential-growth phase but strongly induced in cells at late-exponential-growth phase. we constructed fu ... | 1990 | 2254244 |
| selection of independent plasmids determining phenol degradation in pseudomonas putida and the cloning and expression of genes encoding phenol monooxygenase and catechol 1,2-dioxygenase. | long-term cultivation of the pseudomonas putida multiplasmid strain est1020 on phenol resulted in the formation of individual phe plasmids determining phenol degradation. four types of phe plasmids, pest1024, pest1026, pest1028, and pest1029, are characterized. they all contain a transferrable replicon similar to pwwo-8 with a partly duplicated dna sequence of the 17-kb transposable element of this plasmid and include various amounts of dna that carry genes encoding phenol degradation (phe genes ... | 1990 | 2270227 |
| the 2.1-a resolution structure of iron superoxide dismutase from pseudomonas ovalis. | the 2.1-a resolution crystal structure of native uncomplexed iron superoxide dismutase (ec 1.15.1.1) from pseudomonas ovalis was solved and refined to a final r factor of 24%. the dimeric structure contains one catalytic iron center per monomer with an asymmetric trigonal-bipyramidal coordination of protein ligands to the metal. each monomer contains two domains, with the trigonal ligands (histidines 74 and 160; aspartate 156) contributed by the large domain and stabilized by an extended hydroge ... | 1990 | 2271564 |
| mandelate pathway of pseudomonas putida: sequence relationships involving mandelate racemase, (s)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in escherichia coli. | the genes that encode the five known enzymes of the mandelate pathway of pseudomonas putida (atcc 12633), mandelate racemase (mdla), (s)-mandelate dehydrogenase (mdlb), benzoylformate decarboxylase (mdlc), nad(+)-dependent benzaldehyde dehydrogenase (mdld), and nadp(+)-dependent benzaldehyde dehydrogenase (mdle), have been cloned. the genes for (s)-mandelate dehydrogenase and benzoylformate decarboxylase have been sequenced; these genes and that for mandelate racemase [ransom, s. c., gerlt, j. a ... | 1990 | 2271624 |
| degradation of 3-chlorobiphenyl by in vivo constructed hybrid pseudomonads. | 3-chlorobiphenyl-degrading bacteria were obtained from the mating between pseudomonas putida strain bn10 and pseudomonas sp. strain b13. strains such as bn210 resulted from the transfer of the genes coding the enzyme sequence for the degradation of chlorocatechols from b13 into bn10, whereas b13 derivatives such as b131 have acquired the biphenyl degradation sequence from bn10. during growth of the hybrid strains on 3-chlorobiphenyl 90% chloride was released. activities of phenylcatechol 2,3-dio ... | 1990 | 2276606 |
| cis-1,2-dihydroxycyclohexa-3,5-diene (nad) oxidoreductase (cis-benzene dihydrodiol dehydrogenase) from pseudomonas putida ncib 12190. | 1990 | 2280699 | |
| toluene dioxygenase from pseudomonas putida f1. | 1990 | 2280710 | |
| benzene dioxygenase from pseudomonas putida, ml2 (ncib 12190). | 1990 | 2280718 | |
| [cloning genes for biosynthesis of pseudomonas putida tryptophan in escherichia coli cells]. | the trpe, trpc and trpiba genes of pseudomonas putida were cloned by complementation of the corresponding auxotrophic mutations of escherichia coli using pbr322 as a vector. with the exception of trpe, transcription of all genes in new host takes place under control of their own promoters. expression of the trpd gene linked to trpc was not registered in e. coli. repressible trpc enzyme was synthesized constitutively in e. coli. characteristic regulation of p. putida trpba genes via induction by ... | 1990 | 2283047 |
| comparative in vitro activities of newer quinolones against pseudomonas species and xanthomonas maltophilia isolated from patients with cancer. | the in vitro susceptibilities of three pseudomonas species (pseudomonas aeruginosa, pseudomonas putida, and pseudomonas fluorescens) and xanthomonas maltophilia to quinolone antimicrobial agents were determined. several newer agents, particularly pd117558, pd117596, pd127391, sparfloxacin (at-4140), a-56620, and temafloxacin, were active against pseudomonas species. x. maltophilia isolates were generally less susceptible than were pseudomonas isolates but were inhibited by some of the newer quin ... | 1990 | 2285297 |
| [rare initiation codons are regulators of expression of the rpoc gene]. | translation of the rpoc genes in escherichia coli and salmonella typhimurium is known to start from the gug codon. now, using toeprint analysis we have shown uug to be the initiation codon of the pseudomonas putida rpoc gene. if3 does not seem to proofread initiation at the uug codon. the rpoc genes of p. putida, e. coli, and s. typhimurium, which use rare start codons, have strong sd-domains aggagg (p. p.) and gggag (e. c., s. t.), optimal seven-nucleotide spacing between sd and start codons, a ... | 1990 | 2285427 |
| three isozymes of catechol 1,2-dioxygenase (pyrocatechase), alpha alpha, alpha beta, and beta beta, from pseudomonas arvilla c-1. | three isozymes of catechol 1,2-dioxygenase (pyrocatechase) from pseudomonas arvilla c-1 were separated using deae-toyopearl chromatography. the specific activities of each isozyme were similar to one another. the molecular weights of isozymes 1, 2, and 3 were estimated to be approximately 67,000, 64,000, and 59,000, respectively, from gel filtration. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, isozymes 1 and 3 gave a single protein band, corresponding to mr = 32,000 and 30,000, ... | 1990 | 2295613 |
| protein components of a cytochrome p-450 linalool 8-methyl hydroxylase. | the cytochrome p-450 heme-thiolate monooxygenases that hydroxylate monoterpene hydrocarbon groups are effective models for the cytochrome p-450 family. we have purified and characterized the three proteins from a p-450-dependent linalool 8-methyl hydroxylase in pseudomonas putida (incognita) strain ppg777. the proteins resemble the camphor 5-exohydroxylase components in chemical and physical properties; however, they show neither immunological cross-reactivity nor catalytic activity in heterogen ... | 1990 | 2295633 |
| dna sequences of genes encoding acinetobacter calcoaceticus protocatechuate 3,4-dioxygenase: evidence indicating shuffling of genes and of dna sequences within genes during their evolutionary divergence. | the dna sequence of a 2,391-base-pair hindiii restriction fragment of acinetobacter calcoaceticus dna containing the pcachg genes is reported. the dna sequence reveals that a. calcoaceticus pca genes, encoding enzymes required for protocatechuate metabolism, are arranged in a single transcriptional unit, pcaefdbchg, whereas homologous genes are arranged differently in pseudomonas putida. the pcag and pcah genes represent separate reading frames respectively encoding the alpha and beta subunits o ... | 1990 | 2298704 |
| biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of pseudomonas oleovorans producing the tol plasmid-specified enzyme toluate-1,2-dioxygenase. | the conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was effected by using escherichia coli and pseudomonas recombinants carrying the xylxyz genes originating from the pseudomonas putida mt-2 tol plasmid, thus producing toluate-1,2-dioxygenase. pseudomonas oleovorans gpo12 recombinants readily produced meta- and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates. | 1990 | 2306096 |
| cloning and expression of genes involved in 4-chlorobiphenyl transformation by pseudomonas testosteroni: homology to polychlorobiphenyl-degrading genes in other bacteria. | the genes of pseudomonas testosteroni strain b-356, specifying the transformation of 4-chlorobiphenyl (4-cb) into 4-chlorobenzoic acid (4-cba) were cloned into pseudomonas putida kt2440 using a broad-host-range cosmid, ppsa842. of 10,000 clones tested, four were able to transform 4-cb. gas chromatographic and mass spectrometric analysis of the catabolic products from two of the 4-cb-transforming clones carrying the hybrid plasmids, pda1 and pda2, demonstrated that pda1 carried a complete set of ... | 1990 | 2311936 |
| streptomyces promoter-probe plasmids that utilise the xyle gene of pseudomonas putida. | 1990 | 2315034 | |
| isolation and partial characterization of an extradiol non-haem iron dioxygenase which preferentially cleaves 3-methylcatechol. | a purification procedure has been developed for an extradiol dioxygenase expressed in escherichia coli, which was originally derived from a pseudomonas putida strain able to grow on toluidine. physical and kinetic properties of the enzyme have been investigated. the enzyme has a subunit mr of 33,500 +/- 2000 by sds/polyacrylamide-gel electrophoresis. gel filtration indicates a molecular mass under non-denaturing conditions of 120,000 +/- 20,000. the n-terminal sequence (35 residues) of the enzym ... | 1990 | 2317207 |
| carbon catabolite regulation of phenylacetyl-coa ligase from pseudomonas putida. | phenylacetyl-coa ligase (pa-coa ligase) from p. putida u is a newly described enzyme involved in the aerobic catabolism of phenylacetic acid. the enzyme was specifically induced when p. putida was grown in a chemically defined medium containing phenylacetic acid as the sole carbon source. the induction of pa-coa ligase was delayed by adding easily metabolizable carbon sources to the medium; the effect was more drastic in the presence of glucose. glucose did not cause catabolic inactivation but r ... | 1990 | 2322284 |
| purification and biochemical characterization of phenylacetyl-coa ligase from pseudomonas putida. a specific enzyme for the catabolism of phenylacetic acid. | a new enzyme, phenylacetyl-coa ligase (amp-forming) (pa-coa ligase, ec 6.2.1-) involved in the catabolism of phenylacetic acid (paa) in pseudomonas putida is described and characterized. pa-coa ligase was specifically induced by paa when p. putida was grown in a chemically defined medium in which phenylacetic acid was the sole carbon source. hydroxyl, methyl-phenylacetyl derivatives, and other paa close structural molecules did not induce the synthesis of this enzyme and neither did acetic, buty ... | 1990 | 2324116 |
| sequence analysis of the huth gene encoding histidine ammonia-lyase in pseudomonas putida. | the complete nucleotide sequence of the huth gene, encoding histidine ammonia-lyase (histidase), in pseudomonas putida atcc 12633 has been determined from the appropriate portions of the hut region that had been cloned into escherichia coli. the resulting dna sequence revealed an open reading frame of 1,530 base pairs, corresponding to a protein subunit of approximate molecular weight 53,600, in the location previously identified for the histidase gene by tn1000 mutagenesis. translation began at ... | 1990 | 2332400 |
| chemotaxis of pseudomonas putida toward chlorinated benzoates. | the chlorinated aromatic acids 3-chlorobenzoate and 4-chlorobenzoate are chemoattractants for pseudomonas putida prs2000. these compounds are detected by a chromosomally encoded chemotactic response to benzoate which is inducible by beta-ketoadipate, an intermediate of benzoate catabolism. plasmid pac27, encoding enzymes for 3-chlorobenzoate degradation, does not appear to carry genes for chemotaxis toward chlorinated compounds. | 1990 | 2339899 |
| genes for two herbicide-inducible cytochromes p-450 from streptomyces griseolus. | streptomyces griseolus atcc 11796 contains two inducible, herbicide-metabolizing cytochromes p-450 previously designated p-450su1 and p-450su2 (p-450cva1 and p-450cvb1, respectively, using nomenclature of nebert et al. [d. w. nebert, m. adesnik, m. j. coon, r. w. estabrook, f. j. gonzalez, f. p. guengerich, i. c. gunsalus, e. f. johnson, b. kemper, w. levin, i. r. phillips, r. sato, and m. r. waterman, dna 6:1-11, 1987]). using antibodies directed against cytochrome p-450su1, its n-terminal amin ... | 1990 | 2345149 |
| purification and characterization of salicylate hydroxylase from pseudomonas putida ppg7. | the salicylate hydroxylase from p. putida ppg7 was purified and characterized. the enzyme appears to be monomeric, and it showed one major band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent mr of 45 kda. the sequence of the first 25 amino acids of salicylate hydroxylase (ppg7) was determined. also, the total amino acid composition of salicylate hydroxylase (ppg7) was obtained and compared with that of the known salicylate hydroxylase from p. putida. | 1990 | 2363715 |
| [the sos-like reactions of pseudomonas putida]. | under ultraviolet radiation of pseudomonas putida 1087 the sos-similar response which is expressed in the inhibition of cell respiration and cell division with the following filamentation is revealed. in the result of introduction of ppe24 and pmh21 plasmids into the cells of p. putida 1087 for inhibition of reca-similar protein the sos-similar response disappears and the basic cell mass dies. | 1990 | 2372559 |
| [new plasmids of herbicide 2,4-dichlorophenoxyacetic acid biodegradation]. | three herbicide 2,4-d metabolizing bacterial strains were isolated from three independent soil samples of estonia. the strains, although belonging to various species, contain 2,4-d degradative plasmids with identical restriction patterns. pest4001 is a 78 kb conjugative plasmid. all pseudomonas putida paw340 2,4-d+ transconjugants obtained a 70 kb plasmid pest4011 - a deletion derivative of the pest4001. the restriction patterns of the plasmids mentioned above are considerably different from tho ... | 1990 | 2373362 |
| the downfield resonances in the 1h nmr spectra of azotobacter vinelandii and pseudomonas putida seven-iron ferredoxins. | pseudomonas putida and azotobacter vinelandii ferredoxins each contain one [4fe-4s] cluster and one [3fe-4s] cluster. their polypeptide chains are nearly identical, differing by only 15 residues out of a total of 106. t1 measurements and temperature dependence studies of the 1h nmr spectrum of each ferredoxin demonstrate that all six resolved downfield resonances are near an iron-sulfur center. the five most downfield resonances are shown to arise from protons on cysteinyl beta-carbons by incorp ... | 1990 | 2373698 |
| [peripheral metabolism of pseudomonal putida transconjugants degrading chloro- and methylaromatic compounds]. | peripheral metabolism was studied in the pseudomonas putida 37cc transconjugant. in the strain grown on benzoate, pyrocatechase (pc) i with a low activity to chlorocatechols was induced, whereas pcii actively decomposing chlorocatechols was induced during its growth on 3-chlorobenzoic acid. the p. putida 37cc transconjugant grown on alpha-methylstyrene (ms) exerted the activity of both metapyrocatechase (mpc) and pc, whereas in the parent strain p. putida r-1 only mpc was involved in the degrada ... | 1990 | 2374510 |
| purification and characterization of d-2-haloacid dehalogenase from pseudomonas putida strain aj1/23. | a d-2-haloacid dehalogenase was isolated and purified to homogeneity from pseudomonas putida strain aj1/23. the enzyme catalysed the stereospecific dehalogenation of the d-isomer of 2-chloropropionate. using a new ion-chromatograph assay, the enzyme was found to catalyse the dehalogenation of short-chain 2-halocarboxylic acids. maximum enzyme activity occurred at ph 9.5 and 50 degrees c and the enzyme was insensitive to most -sh reagents. the enzyme has an mr of about 135,000 and appears to be c ... | 1990 | 2380688 |
| chromium reduction in pseudomonas putida. | reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from pseudomonas putida prs2000. chromate reductase activity was associated with soluble protein and not with the membrane fraction. the crude enzyme activity was heat labile and showed a km of 40 microm cro4(2-). neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells. | 1990 | 2389940 |
| evolutionary differences in chromosomal locations of four early genes of the tryptophan pathway in fluorescent pseudomonads: dna sequences and characterization of pseudomonas putida trpe and trpgdc. | pseudomonas putida possesses seven structural genes for enzymes of the tryptophan pathway. all but one, trpg, which encodes the small (beta) subunit of anthranilate synthase, have been mapped on the circular chromosome. this report describes the cloning and sequencing of p. putida trpe, trpg, trpd, and trpc. in p. putida and pseudomonas aeruginosa, dna sequence analysis as well as growth and enzyme assays of insertionally inactivated strains indicated that trpg is the first gene in a three-gene ... | 1990 | 2404959 |
| microbial metabolism of quinoline and related compounds. v. degradation of 1h-4-oxoquinoline by pseudomonas putida 33/1. | a bacterial strain was isolated with the ability to use 1h-4-oxoquinoline as the sole source of carbon, nitrogen and energy. on the basis of its physiological properties, this isolate was classified as pseudomonas putida. 1h-3-hydroxy-4-oxoquinoline, n-formylanthranilic acid, anthranilic acid and catechol were identified as intermediates in the degradation pathway. the latter was further degraded by ortho-cleavage. the enzymatic conversion of 1h-4-oxoquinoline into 1h-3-hydroxy-4-oxoquinoline re ... | 1990 | 1963786 |
| mechanism of action of urocanase. specific 13c-labelling of the prosthetic nad+ and revision of the structure of its adduct with imidazolylpropionate. | 1. [4-13c]nicotinate was synthesised and used to support the growth of a nicotinate auxotrophic mutant of pseudomonas putida. 13c-nmr spectroscopy of the isolated urocanase confirmed the efficient incorporation of 13c into c4 of the nicotinamide ring of the tightly bound nad+ cofactor. 2. beta-[( 2'-13c]imidazol-4-yl)propionate was synthesised according to known procedures and used for inhibition of the 13c-labelled urocanase. an increase in the absorbance at 330 nm indicated adduct formation be ... | 1990 | 1976515 |
| monoclonal antibodies to ferric pseudobactin, the siderophore of plant growth-promoting pseudomonas putida b10. | monoclonal antibodies to ferric pseudobactin, the siderophore (microbial iron transport agent) of plant growth-promoting pseudomonas putida b10, have been developed. three immunoglobulin g subclass 1-type monoclonal antibodies have been characterized. each antibody appears to be unique on the basis of their reactions with ferric pseudobactin and with culture supernatants from other pseudomonads. none of the three cross-reacts with ferric pseudobactin-type siderophores produced by seven other pse ... | 1990 | 16348116 |
| copper resistance gene homologs in pathogenic and saprophytic bacterial species from tomato. | copper-resistant strains of xanthomonas campestris pv. vesicatoria, pseudomonas cichorii, pseudomonas putida, pseudomonas fluorescens, and a yellow pseudomonas sp. were isolated from tomato plants or seeds. in southern hybridizations, dna from each strain showed homology with the copper resistance (cop) operon previously cloned from pseudomonas syringae pv. tomato pt23. homology was associated with plasmid and chromosomal dna in x. compestris pv. vesicatoria, p. putida, and the yellow pseudomona ... | 1990 | 16348118 |
| specificity of octopine uptake by rhizobium and pseudomonas strains. | the octopine-utilizing strain agrobacterium tumefaciens b6s3 and three nonagrobacteria which had the capacity to utilize this opine were compared for octopine uptake. the characteristics of uptake by rhizobium meliloti a3 and strain b6s3 were similar. in both bacteria, uptake activity was inducible by octopine and by the related opine octopinic acid, and competition assays showed that these two opine substrates were accepted by the same uptake system with an equivalent affinity. cells of pseudom ... | 1990 | 16348194 |
| characterization of the opine-utilizing microflora associated with samples of soil and plants. | microorganisms utilizing an opine as the sole carbon source were recovered from crown gall tumors, soil, and surface-disinfected potato tubers. the effect of the opines octopine, nopaline, succinamopine, and mannopine as selective substrates was compared with that of the auxin indoleacetic acid. selection on octopine and indoleacetic acid favored the fluorescent pseudomonads, whereas mannopine allowed the frequent recovery of agrobacteria. coryneforms which utilized succinamopine or mannopine we ... | 1990 | 16348265 |
| catalase and superoxide dismutase of root-colonizing saprophytic fluorescent pseudomonads. | root-colonizing, saprophytic fluorescent pseudomonads of the pseudomonas putida-p. fluorescens group express similar levels of catalase and superoxide dismutase activities during growth on a sucrose- and amino acid-rich medium. increased specific activities of catalase but not superoxide dismutase were observed during growth of these bacteria on components washed from root surfaces. the specific activities of both enzymes were also regulated during contact of these bacteria with intact bean root ... | 1990 | 16348360 |
| overproduction of glutathione and its derivatives by genetically engineered microbial cells. | in order to improve the biotechnological potentials of escherichia coli cells to produce glutathione, s-d-lactoylglutathione and other gamma-glutamyl compounds, the genes for enzymes [gamma-l-glutamyl-l-cysteine synthetase (gsh a) in e. coli b, glutathione synthetase (gsh b) in e. coli b, glyoxalase i (glo i) in pseudomonas putida] were cloned and amplified in e. coli. e. coli b cells transformed with both gsh a and gsh b genes exhibited a high activity in the synthesis of glutathione and other ... | 1990 | 14545903 |
| long-term survival of and plasmid stability inpseudomonas andklebsiella species and appearance of nonculturable cells in agricultural drainage water. | one year after introduction into agricultural drainage waterpseudomonas fluorescens r2f (rp4),pseudomonas putida cym318 (prk2501), andklebsiella aerogenes nctc418 (pbr322) could be recovered on agar media. survival of the introduced strains depended on competition with the indigenous microflora, the presence of nutrients, and the availability of air.in contrast tok. aerogenes nctc418 (pbr322), bothpseudomonas species lost their plasmids, as indicated by the consistently lower colony counts on se ... | 1990 | 24196361 |
| effect of surface-active pseudomonas spp. on leaf wettability. | different strains of pseudomonas putida and p. fluorescens isolated from the rhizosphere and phyllosphere were tested for surface activity in droplet cultures on polystyrene. droplets of 6 of the 12 wild types tested spread over the surface during incubation, and these strains were considered surface active; strains not showing this reaction were considered non-surface active. similar reactions were observed on pieces of wheat leaves. supernatants from centrifuged broth cultures behaved like dro ... | 1989 | 16347926 |
| novel alterations in plasmid dna associated with aromatic hydrocarbon utilization by pseudomonas putida r5-3. | subcultures of pseudomonas putida r5-3 altered their plasmid dna content in specific ways depending on the particular aromatic hydrocarbon utilized as the sole carbon source. two indigenous plasmids, 115 and 95 kilobases (kb) in size, were observed in r5-3a, which was derived from r5-3 by growth on minimal medium containing p-methylbenzoate as the sole carbon source. when r5-3a was transferred to medium containing m-xylene or toluene, derivative strains were obtained in which the 95-kb plasmid w ... | 1989 | 16347946 |
| molybdenum involvement in aerobic degradation of 2-furoic acid by pseudomonas putida fu1. | an organism identified as pseudomonas putida was isolated from an enrichment culture with 2-furoic acid as its sole source of carbon and energy. the organism contained a 2-furoyl-coenzyme a (coa) synthetase to form 2-furoyl-coa and a 2-furoyl-coa dehydrogenase to form 5-hydroxy-2-furoyl-coa as the first two enzymes involved in the degradation. tungstate, the specific antagonist of molybdate, decreased growth rate and consumption of 2-furoic acid but had no influence on growth with succinate. cor ... | 1989 | 16347977 |
| degradation of acetonitrile by pseudomonas putida. | a bacterium capable of utilizing high concentrations of acetonitrile as the sole source of carbon and nitrogen was isolated from soil and identified as pseudomonas putida. this bacterium could also utilize butyronitrile, glutaronitrile, isobutyronitrile, methacrylonitrile, propionitrile, succinonitrile, valeronitrile, and some of their corresponding amides, such as acetamide, butyramide, isobutyramide, methacrylamide, propionamide, and succinamide as growth substrates. acetonitrile-grown cells o ... | 1989 | 16348008 |
| toxicity of trichloroethylene to pseudomonas putida f1 is mediated by toluene dioxygenase. | trichloroethylene was metabolically activated by toluene dioxygenase to produce toxic effects in pseudomonas putida f1. cytotoxicity was indicated by growth inhibition and by the covalent modification of cellular molecules in p. putida f1 exposed to [c]trichloroethylene. with a toluene dioxygenase mutant, neither growth inhibition nor alkylation of intracellular molecules was observed. | 1989 | 16348039 |
| response of plant-colonizing pseudomonads to hydrogen peroxide. | colonization of plant root surfaces by pseudomonas putida may require mechanisms that protect this bacterium against superoxide anion and hydrogen peroxide produced by the root. catalase and superoxide dismutase may be important in this bacterial defense system. stationary-phase cells of p. putida were not killed by hydrogen peroxide (h(2)o(2)) at concentrations up to 10 mm, and extracts from these cells possessed three isozymic bands (a, b, and c) of catalase activity in native polyacrylamide g ... | 1989 | 16348058 |
| antigenic crossreactivity between bacterial and plant cytochrome p-450 monoxygenases. | although cytochrome p-450 monoxygenases mediate critical reactions in plant microsomes, characterization of their activities has been difficult due to their inherent instability and the lack of a crossreacting p-450 antibody. we have surveyed the effects of protein stabilizing agents on t-cinnamic acid hydroxylase (t-cah), a prominent microsomal p-450, and on total p-450 monoxygenase content. trans-cinnamic acid is the most effective protecting agent for t-cah activity. leupeptin, a broad spectr ... | 1989 | 16666804 |
| phenoxyacetic acid degradation by the 2,4-dichlorophenoxyacetic acid (tfd) pathway of plasmid pjp4: mapping and characterization of the tfd regulatory gene, tfdr. | plasmid pjp4 enables alcaligenes eutrophus jmp134 to degrade 3-chlorobenzoate and 2,4-dichlorophenoxyacetic acid (tfd). plasmid pro101 is a derivative of pjp4 obtained by insertion of tn1721 into a nonessential region of pjp4. plasmid pro101 was transferred by conjugation to several pseudomonas strains and to a. eutrophus aeo106, a cured isolate of jmp134. aeo106(pro101) and some pseudomonas transconjugants grew on tfd. transconjugants with a chromosomally encoded phenol hydroxylase also degrade ... | 1989 | 2914848 |
| isolation of a third lipoamide dehydrogenase from pseudomonas putida. | pseudomonads are the only organisms so far known to produce two lipoamide dehydrogenases (lpds), lpd-val and lpd-glc. lpd-val is the specific e3 component of branched-chain oxoacid dehydrogenase, and lpd-glc is the e3 component of 2-ketoglutarate and possibly pyruvate dehydrogenases and the l-factor of the glycine oxidation system. three mutants of pseudomonas putida, js348, js350, and js351, affected in lpdg, the gene encoding lpd-glc, have been isolated; all lacked 2-ketoglutarate dehydrogenas ... | 1989 | 2914869 |
| demonstration, characterization, and mutational analysis of nahr protein binding to nah and sal promoters. | the nahr gene of plasmid nah7 of pseudomonas putida encodes a 36-kilodalton polypeptide which activates transcription of the nah and sal operons in response to the inducer salicylate. a gel mobility shift assay was used to identify a dna-binding activity which was present only in extracts from either p. putida or escherichia coli containing a functional nahr gene. the binding activity was highly specific for dna containing the nah or sal promoters, but the apparent affinity for the promoters was ... | 1989 | 2914873 |
| effect of degradative plasmid cam-oct on responses of pseudomonas bacteria to uv light. | the effect of plasmid cam-oct on responses to uv irradiation was compared in pseudomonas aeruginosa, in pseudomonas putida, and in pseudomonas putida mutants carrying mutations in uv response genes. cam-oct substantially increased both survival and mutagenesis in the two species. p. aeruginosa strains without cam-oct exhibited much higher uv sensitivity than did p. putida strains. uv-induced mutagenesis of plasmid-free p. putida was easily detected in three different assays (two reversion assays ... | 1989 | 2914880 |
| molecular cloning of salicylate hydroxylase genes from pseudomonas cepacia and pseudomonas putida. | the sal gene encoding pseudomonas cepacia salicylate hydroxylase was cloned and the sal encoding pseudomonas putida salicylate hydroxylase was subcloned into plasmid vector pro2317 to generate recombinant plasmids ptk3 and ptk1, respectively. both cloned genes were expressed in the host pseudomonas aeruginosa pao1. the parental strain can utilize catechol, a product of the salicylate hydroxylase-catalyzed reaction, but not salicylate as the sole carbon source for growth due to a natural deficien ... | 1989 | 2916843 |
| sequence analysis of the lpdv gene for lipoamide dehydrogenase of branched-chain-oxoacid dehydrogenase of pseudomonas putida. | the production of two lipoamide dehydrogenases by pseudomonas is so far unique. one, lpd-val, is the specific e3 component of the branched-chain-oxoacid dehydrogenase and the second, lpd-glc, is the e3 component of 2-oxoglutarate dehydrogenase and the l-factor of the glycine oxidation system. the objective of the present research was to determine the nucleotide sequence of the structural gene for lpd-val in order to compare its deduced amino acid structure with that of other redox-active disulfi ... | 1989 | 2917566 |
| crystal structure of muconolactone isomerase at 3.3 a resolution. | the crystal structure of muconolactone isomerase from pseudomonas putida, a unique molecule with ten 96 amino acid subunits and 5-fold, and 2-fold symmetries, has been solved at 3.3 a resolution. the non-crystallographic symmetries were used to refine the initial single isomorphous replacement phases and produce an interpretable 10-fold averaged map. the backbone trace is complete and confirmed by the amino acid sequence fit. each subunit is composed of a body with two alpha-helices and an antip ... | 1989 | 2926818 |
| analysis of nonpolar insertion mutations in the trfa gene of incp plasmid rk2 which affect its broad-host-range property. | replication of broad-host-range plasmid rk2 requires the protein product(s) of the plasmid-encoded trfa gene to initiate replication at oriv, the vegetative replication origin. the trfa gene contains two translational starts which direct translation of two polypeptides, of 382 and 285 amino acids, which differ by the 97 amino acids at their n-terminus. nonpolar insertions which abolish expression of the larger trfa polypeptide but otherwise retain the trfa gene's normal expression signals severe ... | 1989 | 2495025 |
| selection and characterization of a mutant of the cloned gene for mandelate racemase that confers resistance to an affinity label by greatly enhanced production of enzyme. | the plasmid pscr1 containing the gene for mandelate racemase (ec 5.1.2.2) from pseudomonas putida (atcc 12633) allows pseudomonas aeruginosa (atcc 15692) to grow on (r)-mandelate as its sole carbon source [ransom, s. c., gerlt, j. a., powers, v. m., & kenyon, g. l. (1988) biochemistry 27, 540]; the chromosome of the p. aeruginosa host apparently does not contain the gene for mandelate racemase but does contain genes for the remaining enzymes in the mandelate pathway and enables growth on (s)-man ... | 1989 | 2496759 |
| dna sequence of the tryptophan synthase genes of pseudomonas putida. | genes encoding the 2 subunits of tryptophan synthase in pseudomonas putida have been identified and cloned by their similarity to the corresponding genes in pseudomonas aeruginosa. the deduced amino acid sequences were confirmed by comparison with regions ascertained earlier by protein sequencing. the pseudomonas amino acid sequences are 85% identical for the beta subunit and 70% identical for the alpha subunit. these sequences are compared to those of salmonella typhimurium, where the structure ... | 1989 | 2503057 |
| plasmid control of the pseudomonas aeruginosa and pseudomonas putida phenotypes and of linalool and p-cymene oxidation. | two pseudomonas strains (ppg777 and pag158) were derived from the parent isolate pseudomonas incognita (putida). strain ppg777 resembles the parental culture in growth on linalool as a source of carbon and slight growth on p-cymene, whereas pag158 grows well on p-cymene, but not on linalool or other terpenes tested, and has a p. aeruginosa phenotype. curing studies indicate that linalool metabolism is controlled by an extrachromosomal element whose loss forms a stable strain pag158 with the p-cy ... | 1989 | 2504698 |
| synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads. | the fluorescent pseudomonads are classified as a group, one characteristic of which is that they do not accumulate poly-3-hydroxybutyrate (phb) during nutrient starvation in the presence of excess carbon source. in this paper we show that prototype strains from this subclass, such as pseudomonas aeruginosa, pseudomonas putida, and pseudomonas fluorescens, do accumulate poly-3-hydroxyalkanoates (pha) when grown on fatty acids. these phas are composed of medium-chain-length (c6 to c12) 3-hydroxy f ... | 1989 | 2506811 |
| microbial metabolism of quinoline and related compounds. ii. degradation of quinoline by pseudomonas fluorescens 3, pseudomonas putida 86 and rhodococcus spec. b1. | quinoline catabolism was investigated with different bacterial strains, able to use quinoline as sole source of carbon, nitrogen and energy. some degradation products of quinoline were isolated from the culture fluids and identified. with pseudomonas fluorescens and pseudomonas putida we found 2-oxo-1,2-dihydroquinoline, 8-hydroxy-2-oxo-1,2-dihydroquinoline, 8-hydroxycoumarin and 2,3-dihydroxyphenylpropionic acid as intermediates. with a rhodococcus strain 2-oxo-1,2-dihydroquinoline, 6-hydroxy-2 ... | 1989 | 2514722 |
| factors affecting conjugal transfer of plasmids encoding mercury resistance from pure cultures and mixed natural suspensions of epilithic bacteria. | sixty-five pure cultures of epilithic bacteria were examined for their ability to transfer mercury resistance to pseudomonas aeruginosa; five isolates transferred plasmids encoding mercury resistance with frequencies ranging from 8.4 x 10(-8) to 2.8 x 10(-3) per recipient. two of the plasmids, pqm3 and pqm4, encoded narrow-spectrum mercury resistance, pqm3 also encoded streptomycin resistance, and both plasmids were broad host range. maximum transfer frequencies of epilithic plasmids from pure c ... | 1989 | 2515247 |
| [expression of the phospholipase c gene of pseudomonas aeruginosa in escherichia coli and pseudomonas]. | the plc gene for phospholipase of pseudomonas aeruginosa, able to be transcribed only from its own promoter, has been introduced into escherichia coli, pseudomonas aeruginosa and pseudomonas putida cells in the recombinant plasmid ppms21 of a wide host range. the expression of plc gene in all recipient cells has been shown to be phosphate regulated. the fact emphasizes the identity of pho-regulation systems in escherichia coli and pseudomonas cells. the level of phospholipase activity is similar ... | 1989 | 2515430 |
| selective medium for pseudomonas aeruginosa that uses 1,10-phenanthroline as the selective agent. | the mic of 1,10-phenanthroline for 35 pseudomonas aeruginosa strains was 128 micrograms/ml, whereas 32 micrograms or less per ml inhibited all other microorganisms tested. on the basis of these results, a selective agar for p. aeruginosa which contained 15 g of trypticase soy broth (bbl microbiology systems), 15 g of agar, and 0.1 g of phenanthroline per liter was formulated. forty-four p. aeruginosa strains yielded a mean efficiency of plating on this medium of 79% of the counts obtained on try ... | 1989 | 2515804 |
| growth of genetically engineered pseudomonas aeruginosa and pseudomonas putida in soil and rhizosphere. | the effect of the addition of a recombinant plasmid containing the pgla gene encoding an alpha-1,4-endopolygalacturonase from pseudomonas solanacearum on the growth of pseudomonas aeruginosa and pseudomonas putida in soil and rhizosphere was determined. despite a high level of polygalacturonase production by genetically engineered p. putida and p. aeruginosa, the results suggest that polygalacturonase production had little effect on the growth of these strains in soil or rhizosphere. | 1989 | 2515805 |
| cloning and analysis of genes involved in coenzyme b12 biosynthesis in pseudomonas denitrificans. | cobalamin synthesis probably requires 20 to 30 different enzymatic steps. pseudomonas putida and agrobacterium tumefaciens mutants deficient in cobalamin synthesis (cob have been isolated. in p. putida, cob mutants were identified as being unable to use ethanolamine as a source of nitrogen in the absence of added cobalamin (deamination of ethanolamine requires coenzyme b12 as a cofactor). in a. tumefaciens, cob mutants were simply screened for their reduced cobalamin synthesis. a genomic library ... | 1989 | 2536665 |
| cloning and characterization of a gene encoding an outer membrane protein required for siderophore-mediated uptake of fe3+ in pseudomonas putida wcs358. | in iron-limited environments plant-growth-stimulating pseudomonas putida wcs358 produces a yellow-green fluorescent siderophore called pseudobactin 358. ferric pseudobactin 358 is efficiently taken up by cells of wcs358 but not by cells of another rhizophere-colonizing strain, pseudomonas fluorescens wcs374. a gene bank containing partial sau3a dna fragments from wcs358 was constructed in a derivative of the broad-host-range cosmid plafr1. by mobilization of this gene bank to strain wcs374 a cos ... | 1989 | 2540157 |
| structure of the dnaa region of pseudomonas putida: conservation among three bacteria, bacillus subtilis, escherichia coli and p. putida. | we have cloned from pseudomonas putida a gene homologous to escherichia coli dnaa, and determined the sequence of the gene and its neighboring region. the dnaa gene and at least three other genes, dnan, recf and gyrb, were found to be highly homologous to the genes in the dnaa regions of the e. coli and bacillus subtilis chromosomes. a non-translatable region of some 600 bp immediately upstream of the dnaa gene is also conserved in the three bacteria and contains 3, 12, and 14 dnaa-boxes (ttatcc ... | 1989 | 2540413 |
| omegon-km: a transposable element designed for in vivo insertional mutagenesis and cloning of genes in gram-negative bacteria. | to combine the features of the omega interposons with the advantages of in vivo transposition mutagenesis, we have constructed an artificial transposon, called omegon-km. the omegon-km transposon is carried on the plasmid pjff350 which can be conjugally mobilized into a broad range of gram-negative bacteria. omegon-km is flanked, in inverted orientation, by synthetic 28-bp repeats derived from the ends of is1. in addition, each end of omegon-km has the very efficient transcription and translatio ... | 1989 | 2546859 |
| purification and some properties of a 2fe ferredoxin in pseudomonas ovalis. | a [2fe-2s] ferredoxin was found in pseudomonas ovalis which was grown in a medium supplemented with glucose and ammonium sulfate. the molecular weight of the 2fe ferredoxin was estimated to be 13,000. it contained 2.2 gramatoms of non-heme iron and 2.3 gramatoms of acid-labile sulfur per mole protein. the absorption and circular dichroism spectra were characteristic of those of [2fe-2s] type ferredoxins, especially adrenodoxin and putidaredoxin. the electron paramagnetic resonance spectrum of th ... | 1989 | 2548508 |
| a simple procedure for transferring genes cloned in escherichia coli vectors into other gram-negative bacteria: phenotypic analysis and mapping of tol plasmid gene xylk. | a simple method to transfer non-conjugative escherichia coli plasmids to other gram-negative bacteria and their maintenance is described. this method involves generation of inverse transposition-mediated cointegrates of the non-conjugative e. coli plasmid with a conjugative incw broad-host-range plasmid, r388, carrying tn10. isolation of such cointegrates was readily effected by conjugal transfer from an e. coli donor containing the two plasmids to an e. coli recipient, with selection for transc ... | 1989 | 2548929 |
| characterization of five genes in the upper-pathway operon of tol plasmid pww0 from pseudomonas putida and identification of the gene products. | the upper operon of the tol plasmid pww0 of pseudomonas putida encodes a set of enzymes which transform toluene and xylenes to benzoate and toluates. the genetic organization of the operon was characterized by cloning of the upper operon genes into an expression vector and identification of their products in escherichia coli maxicells. this analysis showed that the upper operon contains at least five genes in the order of xylc-xylm-xyla-xylb-xyln. between the promoter of the operon and xylc, the ... | 1989 | 2549010 |
| cloning of pmol28-encoded nickel resistance genes and expression of the genes in alcaligenes eutrophus and pseudomonas spp. | the 163-kilobase-pair (kb) plasmid pmol28, which determines inducible resistance to nickel, cobalt, chromate, and mercury salts in its native host alcaligenes eutrophus ch34, was transferred to a derivative of a. eutrophus h16 and subjected to cloning procedures. after tn5 transposon mutagenesis, restriction endonuclease analysis, and dna-dna hybridization, two dna fragments, a 9.5-kb kpni fragment and a 13.5-kb hindiii fragment (hki), were isolated. hki contained ek1, the kpni fragment, as a su ... | 1989 | 2549012 |
| the purification and characterization of 4-ethylphenol methylenehydroxylase, a flavocytochrome from pseudomonas putida jd1. | the enzyme 4-ethylphenol methylenehydroxylase was purified from pseudomonas putida jd1 grown on 4-ethylphenol. it is a flavocytochrome c for which the mr was found to be 120,000 by ultracentrifuging and 126,000 by gel filtration. the enzyme consists of two flavoprotein subunits each of mr 50,000 and two cytochrome c subunits each of mr 10,000. the redox potential of the cytochrome is 240 mv. hydroxylation proceeds by dehydrogenation and hydration to give 1-(4'-hydroxyphenyl)ethanol, which is als ... | 1989 | 2556994 |