Publications

adaptation of model genetically engineered microorganisms to lake water: growth rate enhancements and plasmid loss.	when a genetically engineered microorganism (gem) is released into a natural ecosystem, its survival, and hence its potential environmental impact, depends on its genetic stability and potential for growth under highly oligotrophic conditions. in this study, we compared plasmid stability and potential for growth on low concentrations of organic nutrients of strains of pseudomonas putida serving as model gems. plasmid-free and plasmid-bearing (nah7) prototrophic isogenic strains and two amino-aci ...	1992	1482185
[electron-conformational interactions at the active site of reduced bacterial cytochrome p450cam induced by a substrate and analysis of the electron structure of heme].	magnetic circular dichroism (mcd) spectra in the soret region (360-480 nm) of camphor-free and camphor-bound reduced bacterial cytochrome p450cam from pseudomonas putida were recorded and analysed in the temperature range from 2 k to 290 k. the temperature dependences of the mcd intensity are qualitatively changed by binding of substrate to the enzyme. in the absence of camphor the linear increase of the mcd intensity with 1/t at t < 4.2 k gives evidence for degeneracy or near degeneracy of the ...	1992	1491671
nucleotide sequence analysis and comparison of the lexa genes from salmonella typhimurium, erwinia carotovora, pseudomonas aeruginosa and pseudomonas putida.	the complete nucleotide sequences of the lexa genes from salmonella typhimurium, erwinia carotovora, pseudomonas aeruginosa and pseudomonas putida were determined; the dna sequences of the lexa genes from these bacteria were 86%, 76%, 61% and 59% similar, respectively, to the escherichia coli k12 gene. the predicted amino acid sequences of the s. typhimurium, e. carotovora and p. putida lexa proteins are 202 residues long whereas that of p. aeruginosa is 204. two putative lexa repressor binding ...	1992	1494343
slow rehydration improves the recovery of dried bacterial populations.	slow rehydration of bacteria from dried inoculant formulations provided higher viable counts than did rapid rehydration. estimates were higher when clay and peat powder formulations of rhizobium meliloti, rhizobium leguminosarum biovar trifolii, and pseudomonas putida, with water activities between 0.280 and 0.650, were slowly rehydrated to water activities of approximately 0.992 before continuing the dilution plating sequence. rhizobium meliloti populations averaged 6.8 x 10(8) cfu/g and 1328 c ...	1992	1504917
degradation of the herbicide bromoxynil in pseudomonas putida.	biological conversion of the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) was studied in a batch culture of pseudomonas putida by using hplc. the process had a cometabolic character and proceeded only in the presence of another, simultaneously metabolizable, carbon and energy source. the intensity of degradation correlated with the growth rate, the degradation stopping when the cosubstrate becomes exhausted or the ph value of the medium falls below 6.5. in a medium with glucose, no l ...	1992	1505868
effect of glucose and ribose on microbial degradation of the herbicide bromoxynil continuously added to soil.	bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) was continuously added to chernozem (haplic typic) soil inoculated with a suspension of pseudomonas putida capable of cometabolic decomposition of the compound in a hetero-continuous-flow cultivation setup. in the steady state, when glucose or ribose were simultaneously added, 90 and 47% of the added herbicide was degraded per day, respectively. if the saccharides were absent, only 10-27% of the herbicide was decomposed. addition and removal of gluc ...	1992	1505869
microbial oxidation of adamantanone by pseudomonas putida carrying the camphor catabolic plasmid.	intact cells of (+/-)camphor-grown pseudomonas putida, atcc17453(cam), have been shown to oxidize readily the monoketone derivative of cage hydrocarbon adamantane, forming oxygenated products indicative of both biological baeyer-villiger and hydroxylation reactions. formed products were identified as 4-oxahomoadamantan-5-one, 5-hydroxyadamantan-2-one and 1-hydroxy-4-oxahomoadamantan-5-one. minor products formed as a result of secondary reactions were tentatively identified as syn- and anti-1,4-d ...	1992	1510672
lack of homology between two haloacetate dehalogenase genes encoded on a plasmid from moraxella sp. strain b.	two genes encoding haloacetate dehalogenases, h-1 and h-2, are closely linked on a plasmid from moraxella sp. strain b. h-1 predominantly acts on fluoroacetate, but h-2 does not. to elucidate the molecular relationship between the two enzymes, we compared their structural genes. two restriction fragments of the plasmid dna were subcloned on m13 phages and their nucleotide sequences were determined. the sequence of each fragment contained an open reading frame that was identified as the structura ...	1992	1512562
construction of a cassette enabling regulated gene expression in the presence of aromatic hydrocarbons.	a high-level expression cassette has been constructed from a tol plasmid derived from pseudomonas putida carrying all cis- and trans-acting regulatory elements necessary for transcriptional gene activation in the presence of aromatic hydrocarbons such as toluene. foreign dna can be inserted at unique kpni, saci, and ecori sites 7, 13, and 15 nucleotides downstream of a ribosome binding site. the cassette, flanked by bamhi and ecori restriction sites, was inserted into a broad-host-range vector a ...	1992	1513877
multiple periplasmic catalases in phytopathogenic strains of pseudomonas syringae.	phytopathogenic strains of pseudomonas syringae are exposed to plant-produced, detrimental levels of hydrogen peroxide during invasion and colonization of host plant tissue. when p. syringae strains were investigated for their capacity to resist h2o2, they were found to contain 10- to 100-fold-higher levels of total catalase activity than selected strains belonging to nonpathogenic related taxa (pseudomonas fluorescens and pseudomonas putida) or escherichia coli. multiple catalase activities wer ...	1992	1514792
oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction.	pseudomonas putida f1 and pseudomonas sp. strain js150 initiate toluene degradation by incorporating molecular oxygen into the aromatic nucleus to form cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene. when toluene-grown cells were incubated with 2- and 3-nitrotoluene, the major products identified were 2- and 3-nitrobenzyl alcohol, respectively. the same cells oxidized 4-nitrotoluene to 2-methyl-5-nitrophenol and 3-methyl-6-nitrocatechol. escherichia coli jm109(pdtg601), which contains the toluene ...	1992	1514810
microbial metabolism of quinoline and related compounds. xiv. purification and properties of 1h-3-hydroxy-4-oxoquinoline oxygenase, a new extradiol cleavage enzyme from pseudomonas putida strain 33/1.	1h-3-hydroxy-4-oxoquinoline oxygenase was purified to apparent homogeneity from pseudomonas putida strain 33/1 which can use 1h-4-oxoquinoline as sole source of carbon. the molecular mass of the enzyme was determined to 26,000 da by gel chromatography and by sds polyacrylamide gel electrophoresis. the enzyme is labile at temperatures above 30 degrees c and has a ph optimum of 8.0. it requires oxygen for the reaction and is significantly inhibited by metal ions like cu2+, zn2+, hg2+ and by 4-chlo ...	1992	1515060
a study of the incidence of different fluorescent pseudomonas species and biovars in the microflora of fresh and spoiled meat and fish, raw milk, cheese, soil and water.	of 182 various foodstuffs and environmental samples examined, 86% had microflora containing fluorescent pseudomonas in differing proportions. a computer-aided technique was used to identify most of the 445 fluorescent strains. pseudomonas fluorescens biovar v-1 was most frequently isolated (24%); it either predominated or was present in all types of samples. other strains, belonging to the other subgroups of biovar v (v-2, v-4, v-5, v-6 and v-7), together represented 14.3%. we also identified ps ...	1992	1517169
degradation of chlorotoluenes by in vivo constructed hybrid strains: problems of enzyme specificity, induction and prevention of meta-pathway.	the activities of the tol plasmid-coded xylene oxygenase, benzylalcohol dehydrogenase, benzaldehyde dehydrogenase of pseudomonas putida strain paw1 were tested with substituted toluenes, benzylalcohols and benzaldehydes, respectively, as substrates. several chlorinated toluenes were shown to induce enzymes of the xylene degradation sequence. conjugative transfer of the tol plasmid from pseudomonas putida strain paw1 to pseudomonas sp. strain b13 and pseudomonas cepacia strain jh230 allowed the i ...	1992	1526468
molecular cloning and characterization of catechol 2,3-dioxygenases from biphenyl/polychlorinated biphenyls-degrading bacteria.	catechol 2,3-dioxygenases were cloned from alcaligenes sp. kf711, pseudomonas putida kf715, and achromobacter xylosoxidans kf701 which are biphenyl/polychlorinated biphenyls-degrading bacteria. all of the cloned enzymes were purified by preparative polyacrylamide gel electrophoresis (page). the purified catechol 2,3-dioxygenases were significantly different from one another in ring-fission activities to catechol and its derivatives. the catechol 2,3-dioxygenase from alcaligenes sp. kf711 exhibit ...	1992	1530619
dna homology between siderophore genes from fluorescent pseudomonads.	many species of pseudomonads produce fluorescent siderophores involved in iron uptake. we have investigated the dna homology between the siderophore synthesis genes of an opportunist animal pathogen, pseudomonas aeruginosa, and three plant-associated species pseudomonas syringae, pseudomonas putida and pseudomonas sp. b10. there is extensive homology between the dna from the different species, consistent with the suggestion that the different siderophore synthesis genes have evolved from the sam ...	1992	1532617
cytochrome p450cam: crystallography, oxygen activation, and electron transfer.	several crystal structures of various substrate and inhibited complexes of the camphor monoxygenase, cytochrome p450cam from pseudomonas putida, are now available. these structures, together with mutagenesis, biochemical, and biophysical studies, have allowed for a detailed penetration into the problem of how p450s activate molecular oxygen, control stereoselectivity, and transfer electrons. this review will provide a summary of the crystallographic work in light of what these structures have ta ...	1992	1537455
sequence and complementation analysis of recf genes from escherichia coli, salmonella typhimurium, pseudomonas putida and bacillus subtilis: evidence for an essential phosphate binding loop.	we have compared the recf genes from escherichia coli k-12, salmonella typhimurium, pseudomonas putida, and bacillus subtilis at the dna and amino acid sequence levels. to do this we determined the complete nucleotide sequence of the recf gene from salmonella typhimurium and we completed the nucleotide sequence of recf gene from pseudomonas putida begun by fujita et al. (1). we found that the recf proteins encoded by these two genes contain respectively 92% and 38% amino acid identity with the e ...	1992	1542576
characterization of catechol 2,3-dioxygenases.	three catechol 2,3-dioxygenases for biphenyl, naphthalene/salicylate, and toluene/xylene oxidation were cloned from achromobacter xylosoxidans kf701, pseudomonas putida (nah7), and pseudomonas sp. (pwwo). the cloned catechol 2,3-dioxygenases were identified by enzymatic activity assay in addition to yellow bands on polyacrylamide gel after electrophoresis and activity staining. all of the cloned catechol 2,3-dioxygenases exhibited their highest activities on catechol as a substrate compared with ...	1992	1543511
the influence of temperature on the behaviour of mixed bacterial contamination of the shell membrane of the hen's egg.	the inner membrane of the air cell of hens' eggs was inoculated with pseudomonas putida, staphylococcus xylosus, enterococcus faecalis, escherichia coli and salmonella enteritidis. the first mentioned eventually dominated the contamination of the albumen of eggs stored at 4, 15, and 20 degrees c. the last mentioned did so in eggs stored at 37 degrees c. the interval between inoculation of the membrane and gross contamination of the albumen was markedly influenced by site of contamination relativ ...	1992	1547832
identification of a cocaine esterase in a strain of pseudomonas maltophilia.	a strain of pseudomonas maltophilia (termed mb11l) which was capable of using cocaine as its sole carbon and energy source was isolated by selective enrichment. an inducible esterase catalyzing the hydrolysis of cocaine to ecgonine methyl ester and benzoic acid was identified and purified 22-fold. in the presence of the solubilizing agent cholate, cocaine esterase had a native mr of 110,000 and was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be a monomer. in the absence ...	1992	1551831
genes and their organization in the replication origin region of the bacterial chromosome.	genes and their organization are conserved in the replication origin region of the bacterial chromosome. to determine the extent of the conserved region in gram-positive and gram-negative bacteria, which diverged 1.2 billion years ago, we have further sequenced the region upstream from the dnaa genes in bacillus subtilis and pseudomonas putida. fifteen open reading frames (orfs) and 11 orfs were identified in the 13.6 kb and the 9.8 kb fragments in b. subtilis and p. putida, respectively. eight ...	1992	1552862
cloning and partial sequencing of an operon encoding two pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity.	we have cloned fragments of dna (up to 13 kb), from pseudomonas putida aj1, that code for two stereospecific haloalkanoate dehalogenases. these enzymes are highly specific for d and l substrates. the two genes, designated hadd and hadl, have been isolated and independently expressed in escherichia coli and p. putida hosts by using broad-host-range vectors. they are closely adjacent and inducible in what appears to be an operon with an upstream open reading frame of unknown function. nucleotide s ...	1992	1556080
purification of pro- and eukaryotic superoxide dismutases by charge-controlled hydrophobic chromatography.	the process of purifying superoxide dismutases was simplified using charge-controlled hydrophobic chromatography on 10-carboxydecyl sepharose. in only one chromatographic step following ammonium sulphate precipitation, fe-containing superoxide dismutase from pseudomonas putida and cu,zn-containing superoxide dismutase from bovine erythrocytes were purified with an overall yield of about 70% to electrophoretic homogeneity. the specific activities of the crystalline enzyme preparations were expres ...	1992	1560096
nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in pseudomonas strain lb400.	the dna region encoding biphenyl dioxygenase, the first enzyme in the biphenyl-polychlorinated biphenyl degradation pathway of pseudomonas species strain lb400, was sequenced. six open reading frames were identified, four of which are homologous to the components of toluene dioxygenase from pseudomonas putida f1 and have been named bpha, bphe, bphf, and bphg. from this comparison, biphenyl dioxygenase was found to be a multicomponent enzyme containing a two-subunit iron-sulfur protein, a ferredo ...	1992	1569021
resonance raman investigations of escherichia coli-expressed pseudomonas putida cytochrome p450 and p420.	high-resolution resonance raman spectra of the ferric, ferrous, and carbonmonoxy (co)-bound forms of wild-type escherichia coli-expressed pseudomonas putida cytochrome p450cam and its p420 form are reported. the ferric and ferrous species of p450 and p420 have been studied in both the presence and absence of excess camphor substrate. in ferric, camphor-bound, p450 (mos), the e. coli-expressed p450 is found to be spectroscopically indistinguishable from the native material. although substrate bin ...	1992	1581294
evaluation of the 4-hour rapid nf plus method for identification of 345 gram-negative nonfermentative rods.	the ability of the rapid nf plus system (innovative diagnostic systems, inc., atlanta, ga.) to identify 345 nonfermentative gram-negative rods was evaluated. kits were inoculated with no. 1 mcfarland suspensions, and reactions were interpreted after a 4-h incubation at 35 degrees c. overall, the method correctly identified 311 strains (90.1%) without additional tests and 21 strains (6.1%) with additional tests, and 13 strains (3.8%) were misidentified. five of 13 misidentified strains were alcal ...	1992	1583129
[the destruction of diethylene glycol by a pseudomonas putida bs-2 culture].		1992	1584086
nucleotide sequence of the structural gene encoding a 2-haloalkanoic acid dehalogenase of pseudomonas putida strain aj1 and purification of the encoded protein.	the nucleotide sequence of a gene encoding an l-2-haloalkanoic acid halidohydrolase from pseudomonas putida strain aj1 was determined. the orf (hadl) codes for a polypeptide of 227 amino acids (mr 25,687) which has significant homology to two other l-2-haloalkanoic acid halidohydrolases of pseudomonas sp., dehci and dehcii; these show 38% and 51% amino acid identity respectively to hadl. all three enzymes produce products of an opposite optical configuration to that of the substrates. comparison ...	1992	1588303
expression and regulation of a dnaa homologue isolated from pseudomonas putida.	a gene homologous to the escherichia coli dnaa gene was isolated from pseudomonas putida and its transcription was investigated in e. coli as well as in p. putida. in both species the p. putida dnaa gene is transcribed from two promoters, one of which shows strong homology to promoters recognized by the sigma 54 factor found in both bacteria. in e. coli transcription of the p. putida dnaa gene can be repressed by overproduction of e. coli dnaa protein, presumably due to the presence of several d ...	1992	1588913
substrate-specificity of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by tol plasmid pww0. metabolic and mechanistic implications.	the substrate-specificities of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase, encoded by tol plasmid pww0 of pseudomonas putida mt-2, were determined. the rates of benzyl alcohol dehydrogenase-catalysed oxidation of substituted benzyl alcohols and reduction of substituted benzaldehydes were independent of the electronic nature of the substituents at positions 3 and 4. substitutions at position 2 of benzyl alcohol affected the reactivity of benzyl alcohol dehydrogenase: the velocity ...	1992	1590768
analysis of mutations in trfa, the replication initiation gene of the broad-host-range plasmid rk2.	plasmids with mutations in trfa, the gene encoding the replication initiation protein of the broad-host-range plasmid rk2, were isolated and characterized. mutants identified from a nitrosoguanidine bank were defective in supporting the replication of a wild-type rk2 origin in escherichia coli. most of the mutations were clustered in a region of trfa corresponding to the carboxy-terminal quarter of the trfa protein. 5' and 3' deletion mutants of trfa were also constructed. a c-terminal deletion ...	1992	1597426
differential bioavailability of soil-sorbed naphthalene to two bacterial species.	prediction of the fate of hydrophobic organic contaminants in soils is complicated by the competing processes of sorption and biodegradation. to test the hypothesis that sorbed naphthalene is unavailable to degradative microorganisms, we developed a simple kinetic method to examine the rates and extents of naphthalene degradation in soil-free and soil-containing systems in a comparison of two bacterial species. the method is predicated on the first-order dependence of the initial mineralization ...	1992	1599237
isolation and screening of plasmids from the epilithon which mobilize recombinant plasmid pd10.	this study examined the potential of bacteria from river epilithon to mobilize a recombinant catabolic plasmid, pd10, encoding 3-chlorobenzoate degradation and kanamycin resistance. fifty-four mobilizing plasmids were exogenously isolated by triparental matings between strains of pseudomonas putida and epilithic bacteria from the river taff (south wales, united kingdom). frequencies for mobilization ranged from 1.7 x 10(-8) to 4.5 x 10(-3) per recipient at 20 degrees c. the sizes of the mobilizi ...	1992	1599248
crystallization and preliminary x-ray diffraction analysis of p450terp and the hemoprotein domain of p450bm-3, enzymes belonging to two distinct classes of the cytochrome p450 superfamily.	cytochromes p450 are members of a superfamily of hemoproteins that are involved in the metabolism of various physiologic and xenobiotic organic compounds. this superfamily of proteins can be divided into two classes based on the electron donor proximal to the p450: an iron-sulfur protein for class i p450s or a flavoprotein for class ii. the only known tertiary structure of any of the cytochromes p450 is that of p450cam, a class i soluble enzyme isolated from pseudomonas putida (product of the cy ...	1992	1608967
pseudomonas putida kt2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers.	the biosynthesis of poly(3-hydroxyalkanoates) (phas) by pseudomonas putida kt2442 during growth on carbohydrates was studied. phas isolated from p. putida cultivated on glucose, fructose, and glycerol were found to have a very similar monomer composition. in addition to the major constituent 3-hydroxydecanoate, six other monomers were found to be present: 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydodecanoate, 3-hydroxydodecenoate, 3-hydroxytetradecanoate, and 3-hydroxytetradecenoate. the ...	1992	1610179
histidine ammonia-lyase from streptomyces griseus.	histidine ammonia-lyase (histidase; huth) has been purified to homogeneity from streptomyces griseus and the n-terminal amino acid (aa) sequence used to clone the histidase-encoding structural gene, huth. the purified enzyme shows typical saturation kinetics and is inhibited competitively by d-histidine and histidinol phosphate. high concentrations of k.cyanide inactivate huth unless the enzyme is protected by the substrate or histidinol phosphate. on the basis of the nucleotide sequence, the hu ...	1992	1612436
genetic analysis of the agga locus involved in agglutination and adherence of pseudomonas putida, a beneficial fluorescent pseudomonad.	an isolate of pseudomonas putida, which rapidly adheres to plant roots, is agglutinated by a glycoprotein from root surfaces. agglutination is prevented and adherence to the root surface is diminished by tn5 insertion in mutant 5123. two cosmid clones from wild type p. putida and a 2.7-kbp ecori-hindiii subclone present in both cosmid clones restored agglutinable to wild type levels in transconjugants of the nonagglutinable (agg-) tn5 mutant 5123. these three clones increased agglutinability in ...	1992	1617198
analysis of an upstream regulatory sequence required for activation of the regulatory gene xyls in xylene metabolism directed by the tol plasmid of pseudomonas putida.	transcription from the promoter of a positive regulatory gene, xyls, on the tol plasmid of pseudomonas putida is activated by another positive regulator, xylr, in the presence of m-xylene and is dependent on rna polymerase containing the ntra protein (sigma 54). deletion analysis of the upstream region of the xyls gene revealed an upstream regulatory sequence (urs), located between 145 and 188 bp upstream from the transcription start site. the urs is active in either orientation and can be place ...	1992	1620097
acyloin formation by benzoylformate decarboxylase from pseudomonas putida.	whole cells and cell extracts of pseudomonas putida grown in a medium containing ammonium mandelate have the capacity to produce the acyloin compound 2-hydroxypropiophenone when incubated with benzoylformate and acetaldehyde. benzaldehyde and benzyl alcohol were formed as reaction by-products. the enantiomeric excess of the 2-hydroxypropiophenone product was found to be 91 to 92%. the absolute configuration of the enzymatically prepared product at the carbinol carbon was found to be s. the thiam ...	1992	1622241
conversion of cis unsaturated fatty acids to trans, a possible mechanism for the protection of phenol-degrading pseudomonas putida p8 from substrate toxicity.	a trans unsaturated fatty acid was found as a major constituent in the lipids of pseudomonas putida p8. the fatty acid was identified as 9-trans-hexadecenoic acid by gas chromatography, argentation thin-layer chromatography, and infrared absorption spectrometry. growing cells of p. putida p8 reacted to the presence of sublethal concentrations of phenol in the medium with changes in the fatty acid composition of the lipids, thereby increasing the degree of saturation. at phenol concentrations whi ...	1992	1622260
naphthalene degradation via salicylate and gentisate by rhodococcus sp. strain b4.	rhodococcus sp. strain b4, isolated from a soil sample contaminated with polycyclic aromatic hydrocarbons, grows with naphthalene as the sole source of carbon and energy. salicylate and gentisate were identified as intermediates in the catabolism of naphthalene. in contrast to the well-studied catabolic pathway encoded by the nah7 plasmid of pseudomonas putida, salicylate does not induce the genes of the naphthalene-degradative pathway in rhodococcus sp. strain b4. the key enzymes of naphthalene ...	1992	1622263
molecular characterization of the entner-doudoroff pathway in escherichia coli: sequence analysis and localization of promoters for the edd-eda operon.	the nucleotide sequence of the entire escherichia coli edd-eda region that encodes the enzymes of the entner-doudoroff pathway was determined. the edd structural gene begins 236 bases downstream of zwf. the eda structural gene begins 34 bases downstream of edd. the edd reading frame is 1,809 bases long and encodes the 602-amino-acid, 64,446-da protein 6-phosphogluconate dehydratase. the deduced primary amino acid sequences of the e. coli and zymomonas mobilis dehydratase enzymes are highly conse ...	1992	1624451
characterization of the genes encoding beta-ketoadipate: succinyl-coenzyme a transferase in pseudomonas putida.	beta-ketoadipate:succinyl-coenzyme a transferase (beta-ketoadipate:succinyl-coa transferase) (ec 2.8.3.6) carries out the penultimate step in the conversion of benzoate and 4-hydroxybenzoate to tricarboxylic acid cycle intermediates in bacteria utilizing the beta-ketoadipate pathway. this report describes the characterization of a dna fragment from pseudomonas putida that encodes this enzyme. the fragment complemented mutants defective in the synthesis of the coa transferase, and two proteins of ...	1992	1624453
microbial metabolism of quinoline and related compounds. xiii. purification and properties of 1h-4-oxoquinoline monooxygenase from pseudomonas putida strain 33/1.	1h-4-oxoquinoline monooxygenase was purified to homogeneity from pseudomonas putida strain 33/1 which can use 1h-4-oxoquinoline as sole source of carbon and energy. the apparent m(r) of the native enzyme was determined to be 126,000 by gel chromatography. sds polyacrylamide gel electrophoresis of the enzyme revealed one protein band corresponding to m(r) 42,000. the enzyme consists of three probably identical subunits with a relative molecular mass of about 42,000. the enzyme requires oxygen and ...	1992	1627263
characterization of pseudomonas putida mutants unable to catabolize benzoate: cloning and characterization of pseudomonas genes involved in benzoate catabolism and isolation of a chromosomal dna fragment able to substitute for xyls in activation of the tol lower-pathway promoter.	mutants of pseudomonas putida mt-2 that are unable to convert benzoate to catechol were isolated and grouped into two classes: those that did not initiate attack on benzoate and those that accumulated 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (benzoate diol). the latter mutants, represents by strain pp0201, were shown to lack benzoate diol dehydrogenase (bend) activity. mutants from the former class were presumed either to carry lesions in one or more subunit structural genes of benzoate dio ...	1992	1629155
lipoamide dehydrogenase from azotobacter vinelandii. the role of the c-terminus in catalysis and dimer stabilization.	the 10 c-terminal residues are not visible in the crystal structure of lipoamide dehydrogenase from azotobacter vinelandii, but can be observed in the crystal structures of the lipoamide dehydrogenases from pseudomonas putida and pseudomonas fluorescens. in these structures, the c-terminus folds back towards the active site and is involved in interactions with the other subunit. the function of the c-terminus of lipoamide dehydrogenase from a. vinelandii was studied by deletion of 5, 9 and 14 re ...	1992	1633805
biodegradation of mixtures of substituted benzenes by pseudomonas sp. strain js150.	pseudomonas sp. strain js150 was isolated as a nonencapsulated variant of pseudomonas sp. strain js1 that contains the genes for the degradative pathways of a wide range of substituted aromatic compounds. pseudomonas sp. strain js150 grew on phenol, ethylbenzene, toluene, benzene, naphthalene, benzoate, p-hydroxybenzoate, salicylate, chlorobenzene, and several 1,4-dihalogenated benzenes. we designed experiments to determine the conditions required for induction of the individual pathways and to ...	1992	1637161
expression of pseudomonas aeruginosa nitrite reductase in pseudomonas putida and characterization of the recombinant protein.	nitrite reductase from pseudomonas aeruginosa has been successfully expressed in pseudomonas putida. the purified recombinant enzyme contains haem c but no haem d1. nonetheless, like the holoenzyme from ps. aeruginosa, it is a stable dimer (molecular mass 120 kda), and electron transfer to oxidized azurin is biphasic and follows bimolecular kinetics (k1 = 1.5 x 10(5) and k2 = 2.2 x 10(4) m-1.s-1). unlike the chemically produced apoenzyme, recombinant nitrite reductase containing only haem c is w ...	1992	1637357
plasmids with easily excisable xyle cassettes.	two new vectors containing the xyle gene (encoding catechol-2,3-dioxygenase) of pseudomonas putida were constructed that serve as the source of the xyle cassette. these vectors are based on the kanamycin-resistance-encoding plasmid, pkan18. the promoter-less xyle gene is flanked by several restriction enzyme sites that allow for easy excision of this gene in the form of a cassette containing a ribosome-binding site, 7 bp upstream from the start codon. these cassettes lack any transcriptional ter ...	1992	1644310
substrate recognition sites in cytochrome p450 family 2 (cyp2) proteins inferred from comparative analyses of amino acid and coding nucleotide sequences.	the substrate recognition regions in cytochrome p450 family 2 (cyp2) proteins were inferred by group-to-group alignment of cyp2 sequences and those of bacterial p450s, including pseudomonas putida p450 101a (p450cam), whose substrate-binding residues have been definitely identified by x-ray crystallography of a substrate-bound form (poulos t. l., finzel, b. c., and howard, a. j. (1987) j. mol. biol. 195, 687-700). the six putative substrate recognition sites, srss, thus identified are dispersive ...	1992	1730627
cis-diol dehydrogenases encoded by the tol pww0 plasmid xyll gene and the acinetobacter calcoaceticus chromosomal bend gene are members of the short-chain alcohol dehydrogenase superfamily.	in the aerobic degradation of benzoate by bacteria, benzoate is first dihydroxylated by a ring-hydroxylating dioxygenase to form a cis-diol (1,2-dihydroxycyclohexa-3,4-diene carboxylate) which is subsequently transformed to a catechol by an nad(+)-dependent cis-diol dehydrogenase. the structural gene for this dehydrogenase, encoded on tol plasmid pww0 of pseudomonas putida (xyll) and that encoded on the chromosome of acinetobacter calcoaceticus (bend), were sequenced. they encode polypeptides of ...	1992	1740120
degradation of phenol and phenolic compounds by pseudomonas putida ekii.	the phenol-degrading strain pseudomonas putida ekii was isolated from a soil enrichment culture and utilized phenol up to 10.6 mm (1.0 g.l-1) as the sole source of carbon and energy. furthermore, cresols, chlorophenols, 3,4-dimethylphenol, and 4-chloro-m-cresol were metabolized as sole substrates by phenol-grown resting cells of strain ekii. under conditions of cell growth, degradation of these xenobiotics was achieved only in co-metabolism with phenol. phenol hydroxylase activity was detectable ...	1992	1368244
maintenance and killing efficiency of conditional lethal constructs in pseudomonas putida.	conditional lethal (suicidal) genetic constructs were designed and employed in strains of pseudomonads as models for containment of genetically-engineered microbes that may be deliberately released into the environment. a strain of pseudomonas putida was formed with a suicide vector designated pbap24h that was constructed by cloning the host killing gene (hok) into the rsf1010 plasmid pvdtac24 and placing it under the control of the tac promoter. after hok induction in p. putida only 40% of surv ...	1992	1368479
purification and characterization of a dna-dependent rna polymerase from pseudomonas putida.	dna-dependent rna polymerase (ec 2.7.7.6) was purified from pseudomonas putida. the enzyme had the typical composition of beta',beta,alpha, and sigma subunits of eubacterial rna polymerases. the molecular masses of the subunits were 156,000 da, 151,000 da, 87,000 da, and 42,000 da, respectively, as measured by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the nh2-terminal amino acid residues of the alpha subunit had a marked homology with those of the alpha subuni ...	1992	1369075
differential siderophore utilization and iron uptake by soil and rhizosphere bacteria.	the differential availabilities of the hydroxamate siderophores ferrioxamine b (fob) and ferrichrome (fc) and the pseudobactin siderophores st3, 7nsk(2), and wcs 358 as sources of fe for soil and rhizosphere bacteria were studied. about 20% of the total bacterial cfu from the rhizospheres of four plant species were able to use fob as the sole fe source in an fe-deficient medium, while about 12, 10, 2, and > 1% were able to use fc and pseudobactins 7nsk(2), st3, and wcs 358, respectively. of the ...	1992	16348618
nitroaromatics are substrates for the tol plasmid upper-pathway enzymes.	expression of the xylma genes encoding for toluene monoxygenase from the lactose promoter in a broad-host-range plasmid allows the oxidation of toluene and m- and p-nitrotoluene to their corresponding benzyl alcohols and benzaldehydes in pseudomonas putida and escherichia coli. benzyl alcohols accumulate until reaching a concentration of about 80 mum, while benzaldehydes accumulate steadily with time for at least 24 h. tol-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase recog ...	1992	16348637
factors affecting 2-hydroxypropiophenone formation by benzoylformate decarboxylase from pseudomonas putida.	benzoylformate (100 mm) was quantitatively converted to the acyloin compound, 2-hydroxypropiophenone (61.76 mm) and benzaldehyde (38.2 mm) by an enzyme extract from pseudomonas putida atcc 12633 in the presence of 1.6m acetaldehyde. biotransformations were carried out at ph 6.0 and 30 degrees c with an incubation time of 60 min. activity of the acyloin forming enzyme, benzoylformate decarboxylase, was 1.23 units/ml in the biotransformation mixture. acyloin formation increased dramatically with p ...	1992	18600905
biodegradation of chlorophenol mixtures by pseudomonas putida.	the dynamic growth behavior of pseudomonas putida has been studied when resting calls were inoculated into a growth medium containing inhibitory concentrations of mixtures of phenol and monochlorophenols. resting cells inoculated into single carbon substrate media did not demonstrate enhanced cell lysis by any of the phenol substrates. the apprarent death rate was reduced as the concentrations of phenol or chlorophenols were increased. this behavior was modeled by employing a constant specific d ...	1992	18601091
enhanced bacterial metabolism of a pseudomonas strain in response to the addition of culture filtrate of a bacteriophagous amoeba.	in a previous work, levrat et al. [21] showed an enhancement of the production of pyoverdin (siderophore) by pseudomonas putida in the presence of amoeba. to explain the mechanism of stimulation, the hypothesis of production of stimulatory factors by amoeba was proposed. filtrates of both mixed culture of bacteria and amoeba (pseudomonas putida + acanthamoeba castellanii) and of axenic culture of amoeba were added to the culture medium of pseudomonas. the production of pyoverdin was increased in ...	1992	23194985
a plant selectable marker gene based on the detoxification of the herbicide dalapon.	a gene from pseudomonas putida coding for a dehalogenase capable of degrading 2,2 dichloropropionic acid (2,2dcpa), the active ingredient of the herbicide dalapon, has been isolated and characterised. in plant transformation experiments the gene was shown to confer resistance to 2,2dcpa at a tissue culture level where 2,2dcpa could be used to select for transformants. at the whole plant level, transformed plants showed resistance to 2,2dcpa at concentrations up to 5 times the recommended dose ra ...	1992	24213366
development of pure culture biofilms of p. putida on solid supports.	pseudomonas putida biofilms were developed on and biofilm accumulation rate data were obtained for the following two classes of support materials: charged surfaces and noncharged hydrophobic and hydrophilic surfaces. the effects of surface roughness and porosity on the rate of microbial attachment were also examined. materials bearing a net positive or negative surface charge supported the greatest biofilm accumulation and the highest biofilm accumulation rate. uncharged hydrophobic materials ac ...	1991	18600638
effects of carbon and oxygen limitations and calcium concentrations on biofilm removal processes.	bacterial biofilm removal processes due to shear and catastrophic sloughing have been investigated in a turbulent flow system under conditions of carbon versus oxygen substrate limitations and varying aqueous phase calcium concentrations. biofilm cellular and extracellular polymer carbon, total biofilm carbon and mass, and biofilm calcium concentrations are measured for pure culture biofilms of the facultative aerobe, pseudomonas putida atcc 11172. results indicate oxygen-limited biofilms reach ...	1991	18597303
use of fluorometry for monitoring and control of a bioreactor.	a new fluorescent bioreactor monitoring probe-multiple excitation fluorometric system (mefs)-has been developed. this probe was compared to the commercially available biochem technology fluromeasure system (nadh probe). in this task the fluorescence behavior of three model fermentation systems, ethanol fermentation by candida utilis, phenol fermentation by pseudomonas putida, and glucose fermentation by saccharomyces cerevisiae, were examined. the results indicated that the fluorescence intensit ...	1991	18597335
lewis cell studies to determine reactor design data for two-liquid-phase bacterial and enzymic reactions.	substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified lewis cells. these measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig liver esterase and toluene oxidation by a strain of pseudomonas putida occur uniformly throughout the bulk of the aqueous phase. such data may be used to provide a basis for two-li ...	1991	18597350
flavonoids released naturally from alfalfa seeds enhance growth rate of rhizobium meliloti.	alfalfa (medicago sativa l.) releases different flavonoids from seeds and roots. imbibing seeds discharge 3',4',5,7-substituted flavonoids; roots exude 5-deoxy molecules. many, but not all, of these flavonoids induce nodulation (nod) genes in rhizobium meliloti. the dominant flavonoid released from alfalfa seeds is identified here as quercetin-3-o-galactoside, a molecule that does not induce nod genes. low concentrations (1-10 micromolar) of this compound, as well as luteolin-7-o-glucoside, anot ...	1991	16668056
effects of 2,4-dichlorophenol, a metabolite of a genetically engineered bacterium, and 2,4-dichlorophenoxyacetate on some microorganism-mediated ecological processes in soil.	a genetically engineered microorganism, pseudomonas putida ppo301(pro103), and the plasmidless parent strain, ppo301, were added at approximately 10 cfu/g of soil amended with 500 ppm of 2,4-dichlorophenoxyacetate (2,4-d) (500 mug/g). the degradation of 2,4-d and the accumulation of a single metabolite, identified by gas chromatography-mass spectrophotometry as 2,4-dichlorophenol (2,4-dcp), occurred only in soil inoculated with ppo301(pro103), wherein 2,4-dcp accumulated to >70 ppm for 5 weeks a ...	1991	16348408
rapid immunocapture of pseudomonas putida cells from lake water by using bacterial flagella.	monoclonal antibodies to pseudomonas putida paw340 cells were produced. in an enzyme-linked immunosorbent assay (elisa) against whole bacterial cells, a hybridoma cell line termed mlv1 produced a monoclonal antibody that reacted with p. putida paw340 but showed no cross-reaction with 100 medical isolates and 150 aquatic isolates. by elisa, immunogold electron microscopy, and western blot (immunoblot) analysis, mlv1 antibody was found to react with purified bacterial flagella. the surfaces of mag ...	1991	16348416
conditional-suicide containment system for bacteria which mineralize aromatics.	a model conditional-suicide system to control genetically engineered microorganisms able to degrade substituted benzoates is reported. the system is based on two elements. one element consists of a fusion between the promoter of the pseudomonas putida tol plasmid-encoded meta-cleavage pathway operon (p(m)) and the laci gene encoding lac repressor plus xyls, coding for the positive regulator of p(m). the other element carries a fusion between the p(tac) promoter and the gef gene, which encodes a ...	1991	16348490
pseudomonas putida which can grow in the presence of toluene.	a pseudomonas putida strain able to grow in the presence of more than 50% toluene was isolated from soil. the strain was tolerant of other toxic solvents, including aliphatic hydrocarbons, alicyclic hydrocarbons, aromatic hydrocarbons, alcohols, and ethers. the stability of the solvent tolerance of strain ih-2000 was stimulated by addition of mg and ca to the medium containing toluene.	1991	16348494
monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in pseudomonas putida f1.	[this corrects the article on p. 2650 in vol. 55.].	1991	16348499
enhancement of the potential to utilize octopine in the nonfluorescent pseudomonas sp. strain 92.	the nonfluorescent pseudomonas sp. strain 92 requires the presence of a supplementary carbon source for growth on octopine, whereas the spontaneous mutant rb100 has acquired the capacity to utilize this opine as the sole carbon and nitrogen source. insertional mutagenesis of rb100 with transposon tn5 generated mutants which were unable to grow on octopine and others which grew slowly on this substrate. both types of mutants yielded revertants that had regained the ability to utilize octopine. so ...	1991	16348533
monitoring a genetically engineered bacterium in a freshwater environment by rapid enzymatic amplification of a synthetic dna "number-plate".	in order to set up a sensitive and reliable detection method to monitor environmentally released genetically engineered microorganisms (gems) a 72-bp, double-stranded dna fragment has been built by annealing and ligating four synthetic oligonucleotides. binding sites for two 20-mer oligonucleotides are situated inside the dna fragment, flanking the centre. into the central part of the construction a 30-nucleotide identification sequence has been fitted. thanks to the presence of the two oligonuc ...	1991	1369367
molecular cloning of a pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates hcl molecules from gamma-hexachlorocyclohexane.	pseudomonas paucimobilis ut26 is capable of growing on gamma-hexachlorocyclohexane (gamma-hch). a genomic library of p. paucimobilis ut26 was constructed in pseudomonas putida by using the broad-host-range cosmid vector pks13. after 2,300 clones were screened by gas chromatography, 3 clones showing gamma-hch degradation were detected. a 5-kb fragment from one of the cosmid clones was subcloned into puc118, and subsequent deletion and gas chromatography-mass spectrometry analyses revealed that a ...	1991	1718942
cloning, sequence and transcriptional analysis of the structural gene for lpd-3, the third lipoamide dehydrogenase of pseudomonas putida.	the third lipoamide dehydrogenase structural gene of pseudomonas putida, lpd3, was isolated from a library of p. putida ppg2 dna cloned in escherichia coli tb1. the nucleotide sequence of lpd3 and its flanking regions indicate that lpd3 is not part of an operon, which is unique for a prokaryotic lipoamide dehydrogenase. an open reading frame was found 207 bases upstream from the start of transcription, but is encoded on the strand opposite lpd3. there is no evidence of an open reading frame imme ...	1991	1722146
[characteristics of the r-plasmid pm3 (incp-9) of a broad circle of hosts].	a new broad host range plasmid pm3 (incp-9) was found in a facultative methylotrophic bacteria pseudomonas putida and described. the pm3 plasmid is characterized by thermo-instability in enterobacteriaceae family of bacteria at 36 degrees c or higher temperatures. it is also unable to be inherited as an autonomous element in the obligate methylotrophic bacteria methylobacillus m75. the peculiarities of plasmid inheritance make possible to use it as a tool for genetic research, for instance, to c ...	1991	1745275
subcloning of bph genes from pseudomonas testosteroni b-356 in pseudomonas putida and escherichia coli: evidence for dehalogenation during initial attack on chlorobiphenyls.	the bpha, -b, -c, and -d genes from pseudomonas testosteroni b-356 were mapped to a 5.5-kb dna fragment of cloned plasmids pda1 and pda2 by use of deletion and insertion mutants of these plasmids. the expression of each of these genes was evaluated in escherichia coli and in pseudomonas putida, and it was found that the bphc and bphd genes are well expressed in both e. coli and p. putida cells while the bpha and bphb genes are very poorly expressed in e. coli, even when placed downstream of a ta ...	1991	1746948
septicaemia and septic arthritis due to pseudomonas putida in a neutropenic patient.		1991	1753153
"in vitro" synthesis of different naturally-occurring, semisynthetic and synthetic penicillins using a new and effective enzymatic coupled system.	forty-seven different penicillins, including some of great clinical importance, have been synthesized "in vitro" by coupling the newly described enzyme phenylacetyl-coa ligase (pcl) from pseudomonas putida and acyl-coa: 6-aminopenicillanic acid (6-apa) acyltransferase (at) from penicillium chrysogenum. incubations were carried out at 30 degrees c in 50 mm hcl-tris buffer ph 8.0. the reaction mixtures contained 6-apa, coa, atp, dithiothreitol, mg2+ and the corresponding penicillin side-chain prec ...	1991	1761422
synthesis of 3-furylmethylpenicillin using an enzymatic procedure.	3-furylmethylpenicillin was synthesized in vitro from 3-furylacetic acid, 6-aminopenicillanic acid (6-apa), coa, atp and mg2+. the reaction was catalyzed in two steps by the enzymes phenyl-acetyl-coa ligase (pcl) from pseudomonas putida and acyl-coa: 6-apa acyltransferase (at) from penicillium chrysogenum. pcl catalyzes the activation of 3-furylacetic acid to 3-furylacetyl-coa (3-f-coa) and at acylates the amino group of 6-apa with the 3-furylacetyl moiety of 3-f-coa, releasing coa and 3-furylme ...	1991	1778415
[cloning of genes degrading 3-chlorobenzoate from pseudomonas putida strain 87].	the ability of pseudomonas putida strain 87 to catabolize 3-chlorobenzoate was shown to be mediated by genes of pbs109 plasmid. the plasmid may be transferred by conjugation into p. aeruginosa pao2175. it seems possible that the pbs109 plasmid codes for pyrocatechase ii specific for halogenated catechol, but not catechol. the genes specifying utilization of 3-chlorobenzoate from pbs109 plasmid were cloned in the 5.5 kb bgiii fragment by using broad-host cloning system. the resulting pbs110 plasm ...	1991	1778448
biotransformation of nitrobenzene by bacteria containing toluene degradative pathways.	nonpolar nitroaromatic compounds have been considered resistant to attack by oxygenases because of the electron withdrawing properties of the nitro group. we have investigated the ability of seven bacterial strains containing toluene degradative pathways to oxidize nitrobenzene. cultures were induced with toluene vapor prior to incubation with nitrobenzene, and products were identified by high-performance liquid chromatography and gas chromatography-mass spectrometry. pseudomonas cepacia g4 and ...	1991	1781679
metabolism of and inhibition by chlorobenzoates in pseudomonas putida p111.	pseudomonas putida p111 was isolated by enrichment culture on 2,5-dichlorobenzoate and was also able to grow on 2-chloro-, 3-chloro-, 4-chloro-, 2,3-dichloro-, 2,4-dichloro-, and 2,3,5-trichlorobenzoates. however, 3,5-dichlorobenzoate completely inhibited growth of p111 on all ortho-substituted benzoates that were tested. when 3,5-dichlorobenzoate was added as a cosubstrate with either 3- or 4-chlorobenzoate, cell yields and chloride release were greater than those observed from growth on either ...	1991	1781694
anaerobic growth and cyanide synthesis of pseudomonas aeruginosa depend on anr, a regulatory gene homologous with fnr of escherichia coli.	anaerobic growth of pseudomonas aeruginosa on nitrate or arginine requires the anr gene, which codes for a positive control element (anr) capable of functionally complementing an fnr mutation in escherichia coli. the anr gene was sequenced; it showed 51% identity with the fnr gene at the amino acid sequence level. four cysteine residues known to be essential in the fnr protein are conserved in anr. the anr gene product (deduced mr 27,129) was visualized by the maxicell method and migrated like a ...	1991	1787798
microbial metabolism of quinoline and related compounds. xii. isolation and characterization of the quinoline oxidoreductase from rhodococcus spec. b1 compared with the quinoline oxidoreductase from pseudomonas putida 86.	quinoline oxidoreductase from rhodococcus spec. b1 was purified 39-fold to apparent homogeneity in a 5-step procedure with a recovery of 26%. the mr of the native enzyme as determined by gel chromatography was 300,000. sds polyacrylamide gel electrophoresis of the enzyme revealed 3 protein bands corresponding to mr 82,000, 32,000, and 18,000. the enzyme contains 1.3 atoms of molybdenum, 8 atoms of iron, 8 atoms of acid-labile sulphur, 2 molecules of fad and 2 molecules of molybdopterin cytosine ...	1991	1789933
phenotypic variation of pseudomonas putida and p. tolaasii affects the chemotactic response to agaricus bisporus mycelial exudate.	the chemotactic response of wild-type pseudomonas putida and p. tolaasii, and a phenotypic variant of each species, to agaricus bisporus mycelial exudate was examined. both p. putida, the bacterium responsible for initiating basidiome development of a. bisporus, and p. tolaasii, the causal organism of bacterial blotch disease of the mushroom, displayed a positive chemotactic response to casamino acids and to a. bisporus mycelial exudate. the response was both dose- and time-dependent and marked ...	1991	1791431
phenotypic variation of pseudomonas putida and p. tolaasii affects attachment to agaricus bisporus mycelium.	the effect of phenotypic variation on attachment of pseudomonas tolaasii and p. putida to agaricus bisporus mycelium was investigated. quantitative studies demonstrated the ability of each isolate to attach rapidly and firmly to a. bisporus mycelium and significant differences in attachment of wild-type and phenotypic variant strains were observed. this was most pronounced in p. tolaasii, where the percentage attachment of the wild-type form was always greater than that of the phenotypic variant ...	1991	1791432
isolation and characterization of pseudomonas putida mutants affected in arginine, ornithine and citrulline catabolism: function of the arginine oxidase and arginine succinyltransferase pathways.	pseudomonas putida mutants impaired in the utilization of arginine are affected in either the arginine succinyltransferase pathway, the arginine oxidase route, or both. however, mutants affected in one of the pathways still grow on arginine as sole carbon source. analysis of the products excreted by both wild-type and mutant strains suggests that arginine is mainly channelled by the oxidase route. proline non-utilizing mutants are also affected in ornithine utilization, confirming the role of pr ...	1991	1791443
dna sequence determination of the tol plasmid (pwwo) xylgfj genes of pseudomonas putida: implications for the evolution of aromatic catabolism.	the meta operon of the pseudomonas putida tol plasmid (pwwo) encodes all enzymes of a meta-cleavage pathway for the metabolism of benzoic acids to krebs-cycle intermediates. we have determined and analysed the nucleic acid sequence of a 3442 bp region of the meta operon containing the xyl-gfj genes whose products are involved in the post meta-ring fission transformation of catechols. homology analysis of the xylgfj gene products revealed evidence of biochemical relatedness, suggested enzymatic m ...	1991	1791759
[the autoselection of neustonic forms of bacteria].	self-breeding of neuston forms of methylobacterium sp., pseudomonas putida bc-2, alcaligenes paradoxus bc-1, bacillus thuringiensis var. israilensis bacteria as well as of a mixed culture of methylotrophs is shown possible. in spite of ability of hydrophobicity of the cell surface the suggested method of self-breeding may be used to perfect properties of larvicidal biopreparations, and bacterial preparations which intensify self-purification of water bodies.	1991	1791780
[quantitation of acinetobacter calcoaceticus in mixed bacterial cultures by an enzyme immunoassay].	an enzyme-linked immunosorbent assay using polyclonal antibodies from rabbits has been developed for quantification of acinetobacter calcoaceticus. bacteria were added to the wells of a microtiter plate coated with anti-acinetobacter immunoglobulin. for detecting bound cells the peroxidase-labelled immunoglobulin fraction was used. over a distinct range there is a linear correlation between bound bacteria and measured absorbance allowing a quantification of bacteria in an order from 10(7) to 10( ...	1991	1793089
highly conserved coding sequences in polychlorinated biphenyl (pcb)-degraders of pseudomonas pseudoalcaligenes kf707 and pseudomonas putida kf715.	sixteen strains that utilize biphenyl or polychlorinated biphenyl (pcb) as the sole source of carbon and energy have previously been isolated. nucleotide sequence of the bphabcxd operon (11 kb) of pseudomonas pseudoalcaligenes kf707 has now been determined. when bphd region is compared with the previously reported bphd sequence of another pcb-degrader, pseudomonas putida kf715, homologies at the level of nucleotides as well as amino acids are as high as 96%. moreover, both bphabcxd operon (kf707 ...	1991	1842046
three-dimensional structure of p-cresol methylhydroxylase (flavocytochrome c) from pseudomonas putida at 3.0-a resolution.	p-cresol methylhydroxylase (pcmh) isolated from pseudomonas putida is an alpha 2 beta 2 tetramer of approximate subunit mr 49,000 and 9,000. it is a flavocytochrome c containing covalently bound fad in the larger subunit and covalently bound heme in the smaller. crystals in space group p2(1)2(1)2(1) with unit-cell parameters a = 140.3 a, b = 130.6 a, and c = 74.1 a contain one full molecule per asymmetric unit and diffract anisotropically to about 2.8-a resolution in two directions and to about ...	1991	1846290
a series of wide-host-range low-copy-number vectors that allow direct screening for recombinants.	a series of controlled expression vectors was constructed based on the wide-host-range plasmid pmmb66eh. some of these new vectors code for the alpha-peptide of beta-galactosidase and allow the direct screening of recombinant clones by inactivation of alpha-complementation. the bla gene was replaced in some plasmids by the cat gene of tn9 coding for chloramphenicol resistance, extending the use into beta-lactam-resistant strains. they all feature either the tac or taclac (tac-lac uv5 in tandem) ...	1991	1847347
[localization of camphor degradative plasmids on the chromosome of pseudomonas putida strains paw].	camphor degradative plasmids (cam, prk1) are preferentially situated on chromosomes of pseudomonas putida strains paw. after having been transferred into cam+ strains, the tol plasmid pwwo dissociates into the cryptic plasmid pwwo-8 and chromosome-borne transposon tn4651. the opposite situation, i.e. reconstruction of the tol plasmid pwwo from the cryptic plasmid pwwo-8 and chromosome-borne catabolic operons of the pwwo plasmid has been described. cam- derivatives of the cam plasmid were obtaine ...	1991	1855659
a predicted three-dimensional structure of human cytochrome p450: implications for substrate specificity.	a three-dimensional structure for human cytochrome p450ia1 was predicted based on the crystal coordinates of cytochrome p450cam from pseudomonas putida. as there was only 15% residue identity between the two enzymes, additional information was used to establish an accurate sequence alignment that is a prerequisite for model building. twelve representative eukaryotic sequences were aligned and a net prediction of secondary structure was matched against the known alpha-helices and beta-sheets of p ...	1991	1857713
binding sites of pyridine in cytochrome p-450cam.	careful titration of oxidized cytochrome p-450cam from pseudomonas putida with pyridine revealed deviations of the eadie plot from linearity in the substrate-bound as well as in the substrate-free protein. a binding model which assumes two binding sites for pyridine--the iron and the camphor binding site--is able to describe completely the nonlinear eadie plot.	1991	1859821
hydroxylation of o-halogenophenol and o-nitrophenol by salicylate hydroxylase.	salicylate hydroxylase [ec 1.14.13.1] from pseudomonas putida catalyzed the formation of catechol from substrate analogues such as o-nitro-, o-amino-, o-iodo-, o-bromo-, and o-chloro-phenol by removing the ortho-substituted groups. they are converted into nitrite, ammonia, and halide ions, respectively. kinetic parameters of these reactions were determined by spectrophotometric and polarographic methods. hydroxylation of o-nitro- or o-iodophenol proceeds with the unusual stoichiometry of 2:1:1 f ...	1991	1864847
degradation of l-djenkolate catalyzed by s-alkylcysteine alpha,beta-lyase from pseudomonas putida.	s-alkylcysteine alpha, beta-lyase [ec 4.4.1.6] of pseudomonas putida catalyzes alpha,beta-elimination of l-djenkolate [3,3'-methylenedithiobis(2-aminopropionic acid)] to produce pyruvate, ammonia, and s-(mercaptomethyl)cysteine initially. secondly, s-(mercaptomethyl)-cysteine, which was identified in the form of s-(mercaptomethyl)cysteine thiolactone and s-(2-thia-3-carboxypropyl)cysteine in the absence and presence of iodoacetic acid, respectively, is decomposed enzymatically to pyruvate, ammon ...	1991	1869519
assay for detection and enumeration of genetically engineered microorganisms which is based on the activity of a deregulated 2,4-dichlorophenoxyacetate monooxygenase.	an assay system was developed for the enumeration of genetically engineered microorganisms expressing a deregulated 2,4-dichlorophenoxyacetate (tfd) monooxygenase, which converts phenoxyacetate (paa) to phenol. in paa-amended cultures of pseudomonas aeruginosa pao1c(pro103) and pseudomonas putida ppo301(pro103), strains which express a deregulated tfd monooxygenase, phenol production was proportional to cell number. phenol was reacted, under specific conditions, with a 4-aminoantipyrine dye to f ...	1991	1872608