Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| sterically stabilized cationic liposomes improve the uptake and immunostimulatory activity of cpg oligonucleotides. | immunostimulatory cpg oligonucleotides (odn) show promise as immune adjuvants, anti-allergens, and immunoprotective agents. increasing the bioavailability and duration of action of cpg odn should improve their therapeutic utility. encapsulating odn in sterically stabilized cationic liposomes provides protection from serum nucleases while facilitating uptake by b cells, dendritic cells, and macrophages. in a pathogen challenge model, sterically stabilized cationic liposomes encapsulation doubled ... | 2001 | 11544321 |
| assessment of the virulence of listeria monocytogenes: agreement between a plaque-forming assay with ht-29 cells and infection of immunocompetent mice. | some listeria monocytogenes strains not related to clinical cases have been found to exhibit a low virulence level in mice as well as in an in vitro test using caco-2 cells. the purpose of this study was to validate a new in vitro test of virulence based on a plaque-forming assay (pfa) using a ht-29 cell monolayer with 118 listeria strains. the use of ht-29 cells in 96-well tissue culture plates allowed the testing of 30 strains per day and providing results in 24 h. in addition. statistical ana ... | 2001 | 11545218 |
| comparison of the efficacy of different techniques, culture media, and sources of blood in determining the hemolytic activity of listeria spp.. | hemolytic activity is a fundamental criterion for the differentiation of listeria species; therefore, a simple and inexpensive procedure to clearly distinguish hemolytic strains from each other and from nonhemolytic strains would be of great aid. we compared the efficacy of several techniques, culture media, and types of blood in demonstrating the hemolysis of listeria spp. the hemolytic activities of listeria monocytogenes and listeria seeligeri were more easily detected with a red blood cell t ... | 2001 | 11547885 |
| [virulence factor expression regulation in listeria monocytogenes]. | the external and internal factors affecting the expression of l. monocytogenes virulence genes are analyzed. the role of protein prfa, the only known factor directly affecting the transcription of virulence genes, as well as the contribution of other regulatory elements modulating the activity and/or intracellular concentration of prfa are discussed. the environmental factors which influence the level of expression, such as temperature and the presence of metabolized carbohydrates in the cultiva ... | 2001 | 11550545 |
| [hemorrhagic syndrome during high-dose ampicillin treatment]. | in high doses, the beta-lactams--particularly cephalosporins--can cause bleeding from impairment of prothrombin formation. we report a case of hemorrhagic syndrome in a 63-year-old woman during therapy with high doses of ampicillin for listeria monocytogenes meningitis. | 2001 | 11552312 |
| fetal death in utero secondary to listeria monocytogenes placental infection. | 2001 | 11552741 | |
| eukaryotic expression plasmid transfer from the intracellular bacterium listeria monocytogenes to host cells. | the facultative intracellular, gram-positive bacterium listeria monocytogenes invades phagocytic and non-phagocytic cells from the tissues and organs of a wide variety of animals and humans. here, we report the use of these bacteria as vehicles for gene transfer. eukaryotic expression plasmids were introduced into the nucleus of host cells following lysis of the intracytosolic, plasmid-carrying bacteria with antibiotics. cell lines of different tissues and species could be transfected in this wa ... | 2001 | 11553012 |
| actin-based motility is sufficient for bacterial membrane protrusion formation and host cell uptake. | shigella flexneri replicates in the cytoplasm of host cells, where it nucleates host cell actin filaments at one pole of the bacterial cell to form a 'comet tail' that propels the bacterium through the host's cytoplasm. to determine whether the ability to move by actin-based motility is sufficient for subsequent formation of membrane-bound protrusions and intercellular spread, we conferred the ability to nucleate actin on a heterologous bacterium, escherichia coli. previous work has shown that i ... | 2001 | 11553015 |
| prevalence of listeria spp and listeria monocytogenes in meat and fermented fish in malaysia. | fermented fish and meat samples were purchased from supermarket and wet market for microbiological analysis of listeria species and listeria monocytogenes contamination. listeria species were isolated from 17 (73.9%) of 23 samples of imported frozen beef, 10 (43.5%) of the 23 samples of local beef and 14 (56%) of the 25 samples of fermented fish from wet market. listeria monocytogenes occurred in 15 (75%) of the frozen beef samples, 6 (30.4%) of the 23 samples of local meat and 3 (12%) of the 25 ... | 2001 | 11556596 |
| evidence for intraaxonal spread of listeria monocytogenes from the periphery to the central nervous system. | rhombencephalitis due to listeria monocytogenes is characterized by progressive cranial nerve palsies and subacute inflammation in the brain stem. in this paper, we report observations made on mice infected with l. monocytogenes. unilateral inoculation of bacteria into facial muscle, or peripheral parts of a cranial nerve, induced clinical and histological signs of mainly ipsilateral rhombencephalitis. similarly, unilateral inoculation of bacteria into lower leg muscle or peripheral parts of sci ... | 2001 | 11556688 |
| inactivation and injury of pressure-resistant strains of escherichia coli o157 and listeria monocytogenes in fruit juices. | to investigate methods for inactivating a pressure-resistant strain of escherichia coli o157 in fruit juices. | 2001 | 11556911 |
| factors affecting production of an antilisterial bacteriocin by carnobacterium piscicola strain a9b in laboratory media and model fish systems. | to investigate factors influencing bacteriocin production and bacteriocin stability of the bioprotective culture carnobacterium piscicola strain a9b. | 2001 | 11556917 |
| characterization of listeria monocytogenes isolated from production lines of fresh and cold-smoked fish. | the aims of this study were to characterize strains of listeria monocytogenes isolated from cold-smoking fish plants to establish possible routes of contamination through the processing chain. | 2001 | 11556924 |
| listeria monocytogenes septicaemia in foals. | 2001 | 11558750 | |
| the differential adherence capabilities of two listeria monocytogenes strains in monoculture and multispecies biofilms as a function of temperature. | to determine the differential adherence capabilities at three different temperatures of listeria monocytogenes scott a, a clinical food pathogen, and l. monocytogenes fm876, a persistent strain from a milk-processing environment, to stainless steel. | 2001 | 11559409 |
| regression of established human papillomavirus type 16 (hpv-16) immortalized tumors in vivo by vaccinia viruses expressing different forms of hpv-16 e7 correlates with enhanced cd8(+) t-cell responses that home to the tumor site. | using vaccinia virus as a live vector, we show that the expression of human papillomavirus type 16 (hpv-16) e7 fused to a nonhemolytic portion of the listeria monocytogenes virulence factor, listeriolysin o (llo), induces an immune response that causes the regression of established hpv-16 immortalized tumors in c57bl/6 mice. the vaccinia virus construct expressing llo fused to e7 (vaclloe7) was compared with two previously described vaccinia virus constructs: one that expresses unmodified e7 (va ... | 2001 | 11559797 |
| listeria protein acta mimics wasp family proteins: it activates filament barbed end branching by arp2/3 complex. | actin-based propulsion of the bacteria listeria and shigella mimics the forward movement of the leading edge of motile cells. while shigella harnesses the eukaryotic protein n-wasp to stimulate actin polymerization and filament branching through arp2/3 complex, the listeria surface protein acta directly activates arp2/3 complex by an unknown mechanism. here we show that the n-terminal domain of acta binds one actin monomer, in a profilin-like fashion, and arp2/3 complex and mimics the c-terminal ... | 2001 | 11560487 |
| reversible acute hydrocephalus complicating listeria monocytogenes meningitis. | 2001 | 11561813 | |
| protective effect of a traditional japanese medicine hochu-ekki-to (chinese name: bu-zhong-yi-qi-tang), on the susceptibility against listeria monocytogenes in infant mice. | in this study, the effect of traditional japanese (chinese) medicine, hochu-ekki-to, hot (chinese name: bu-zhong-yi-qi-tang), on the susceptibility against listeria monocytogenes in postneonatal infant mice was examined. numbers of bacteria in infant mice (infected at 4 weeks of age) were significantly higher than those in adult mice (infected at 8 weeks of age) on day 3 (non-specific resistance phase) and day 5 (specific resistance phase) after infection. oral administration of 1,000 mg/kg of h ... | 2001 | 11562059 |
| role of the glutamate decarboxylase acid resistance system in the survival of listeria monocytogenes lo28 in low ph foods. | the glutamate decarboxylase (gad) acid resistance system of the foodborne pathogen listeria monocytogenes plays a major role in its survival at low ph. it was found that survival of the wild-type strain. lo28, in acidified reconstituted skim milk, diluted to reduce free glutamate levels. improves in response to supplementation with monosodium glutamate. a mutant, in which the two listerial gad homologs have been deleted (and in which there is no discernible gad activity), did not respond to glut ... | 2001 | 11563513 |
| survival of listeria monocytogenes attached to stainless steel surfaces in the presence or absence of flavobacterium spp. | contaminated surfaces of food processing equipment are believed to be a significant source of listeria monocytogenes to foods. however, very little is known about the survival of listeria in processing environments. in a mixed bacterial biofilm of l. monocytogenes and flavobacterium spp., the number of l. monocytogenes cells attaching to stainless steel increased significantly compared to when l. monocytogenes was in a pure culture. the l. monocytogenes cells in the mixed biofilms were also reco ... | 2001 | 11563514 |
| monitoring of microbial hazards at farms, slaughterhouses, and processing lines of swine in korea. | this study was executed to investigate microbiological hazards at swine farms, slaughterhouses, dressing operations, and local markets for the application of the hazard analysis critical control point system in korea by analyzing total aerobic plate count (apc) and presence of pathogens. six integrated pig farms and meat packers were selected from six different provinces, and samples were collected from pig carcasses by swabbing and excision methods at the slaughterhouses, processing rooms, and ... | 2001 | 11563516 |
| combined effects of nacl, naoh, and biocides (monolaurin or lauric acid) on inactivation of listeria monocytogenes and pseudomonas spp. | this study highlighted combinations of chemical stresses that could decrease or eliminate listeria monocytogenes and pseudomonas spp. surviving in food processing plants. strains of l. monocytogenes, pseudomonas fragi, and pseudomonas fluorescens isolated from processing environments (meat and milk) were grown at 20 degrees c up to the early stationary phase. the strains were then subjected to 30 min of physicochemical treatments. these treatments included individual or combined acid (acetic aci ... | 2001 | 11563526 |
| the hazard analysis and critical control points (haccp) generic model for the production of thai fermented pork sausage (nham). | nham is a traditional thai fermented pork sausage. the major ingredients of nham are ground pork meat and shredded pork rind. nham has been reported to be contaminated with salmonella spp., staphylococcus aureus, and listeria monocytogenes. therefore, it is a potential cause of foodborne diseases for consumers. a hazard analysis and critical control points (haccp) generic model has been developed for the nham process. nham processing plants were observed and a generic flow diagram of nham proces ... | 2001 | 11570169 |
| feasibility of fluorescent detection of pathogens on pork carcasses. | the direct immunofluorescent detection of pathogens on pork skin is evaluated. calibrate contamination of pork skin with salmonella typhimurium (st) and listeria monocytogenes (lm) is developed in 2 h at 4 degrees c. then a specific indirect immunofluorescent staining protocol is optimized in order to obtain specific and intensive signals able to be detected by electronic cameras (deported microscopy). despite the individual staining of st and lm is possible on pork skin and is specific and brig ... | 2001 | 11570187 |
| role of sigma(b) in heat, ethanol, acid, and oxidative stress resistance and during carbon starvation in listeria monocytogenes. | to determine the contribution of sigma b (sigma(b)) to survival of stationary-phase listeria monocytogenes cells following exposure to environmental stresses, we compared the viability of strain 10403s with that of an isogenic nonpolar sigb null mutant strain after exposure to heat (50 degrees c), ethanol (16.5%), or acid (ph 2.5). strain viabilities were also determined under the same conditions in cultures that had been previously exposed to sublethal levels of the same stresses (45 degrees c, ... | 2001 | 11571142 |
| mutations in the listerial prob gene leading to proline overproduction: effects on salt tolerance and murine infection. | the observed sensitivity of listeria monocytogenes to the toxic proline analogue l-azetidine-2-carboxylic acid (az) suggested that proline synthesis in listeria may be regulated by feedback inhibition of gamma-glutamyl kinase (gk), the first enzyme of the proline biosynthesis pathway, encoded by the prob gene. taking advantage of the epicurian coli mutator strain xl1-red, we performed random mutagenesis of the recently described proba operon and generated three independent mutations in the liste ... | 2001 | 11571156 |
| internalin b activates nuclear factor-kappa b via ras, phosphoinositide 3-kinase, and akt. | internalin b (inlb), a 630-amino acid protein loosely attached to the surface of listeria monocytogenes, participates in the entry of the bacterium into mammalian cells. this process requires the activation of phosphoinositide (pi) 3-kinase by inlb. previously, we demonstrated that inlb activates the transcription factor nuclear factor-kappab in murine j774 macrophage-like cells, an event that also requires pi 3-kinase. here we have further investigated this phenomenon. inlb activated the small ... | 2001 | 11571285 |
| a role for cofilin and lim kinase in listeria-induced phagocytosis. | the pathogenic bacterium listeria monocytogenes is able to invade nonphagocytic cells, an essential feature for its pathogenicity. this induced phagocytosis process requires tightly regulated steps of actin polymerization and depolymerization. here, we investigated how interactions of the invasion protein inlb with mammalian cells control the cytoskeleton during listeria internalization. by fluorescence microscopy and transfection experiments, we show that the actin-nucleating arp2/3 complex, th ... | 2001 | 11571311 |
| microinjection and growth of bacteria in the cytosol of mammalian host cells. | most facultative intracellular bacteria replicate in specialized phagosomes after being taken up by mammalian cells. relatively few intracellular bacteria escape the phagosomal compartment with the help of cytolytic (pore-forming) proteins and replicate in the host cell cytosol. without such toxins, intracellular bacteria cannot reach this cellular compartment. to circumvent the requirement of an "escape" step, we developed a procedure allowing the efficient direct injection of bacteria into the ... | 2001 | 11572936 |
| internalins from the human pathogen listeria monocytogenes combine three distinct folds into a contiguous internalin domain. | listeria monocytogenes is an opportunistic, food-borne human and animal pathogen. host cell invasion requires the action of the internalins a (inla) and b (inlb), which are members of a family of listerial cell-surface proteins. common to these proteins are three distinctive n-terminal domains that have been shown to direct host cell-specific invasion for inla and inlb. here, we present the high-resolution crystal structures of these domains present in inlb and inlh, and show that they constitut ... | 2001 | 11575932 |
| adsorption, attachment and biofilm formation among isolates of listeria monocytogenes using model conditions. | to determine whether isolates of listeria monocytogenes differ in their ability to adsorb and form biofilms on a food-grade stainless steel surface. | 2001 | 11576310 |
| functional assembly of two membrane-binding domains in listeriolysin o, the cytolysin of listeria monocytogenes. | listeriolysin o (llo) is a major virulence factor secreted by the pathogenic listeria monocytogenes and acts as pore-forming cytolysin. based on sequence similarities between llo and perfringolysin (pfo), the cytolysin from clostridium perfringens of known crystallographic structure, two truncated llo proteins were produced: llo-d123, comprising the first three predicted domains, and llo-d4, the last c-terminal domain. the two proteins were efficiently secreted into the culture supernatant of l. ... | 2001 | 11577147 |
| relationship between nucleic acid ratios and growth in listeria monocytogenes. | listeria monocytogenes is a pathogen whose distribution in a range of foodstuffs requires the development of methods for sensitive and rapid detection. molecular biological methods usually rely on specific detection of l. monocytogenes rdna directly amplified by the application of pcr to dna extracts. information on the metabolic status of l. monocytogenes populations would be valuable and can, in theory, be provided by quantitative detection of rrna itself. both fluorometry and oligonucleotide ... | 2001 | 11577148 |
| pivotal role of vasp in arp2/3 complex-mediated actin nucleation, actin branch-formation, and listeria monocytogenes motility. | the listeria monocytogenes acta protein mediates actin-based motility by recruiting and stimulating the arp2/3 complex. in vitro, the actin monomer-binding region of acta is critical for stimulating arp2/3-dependent actin nucleation; however, this region is dispensable for actin-based motility in cells. here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (vasp) recruitment by acta can bypass defects in actin monomer-binding. furthermore, purified vasp enh ... | 2001 | 11581288 |
| difference in cholesterol-binding and cytolytic activities between listeriolysin o and seeligeriolysin o: a possible role of alanine residue in tryptophan-rich undecapeptide. | we have constructed recombinant listeriolysin o (rllo) and seeligeriolysin o (rlso) from listeria monocytogenes and listeria seeligeri, respectively. in hemolysis and cholesterol-binding assays, the specific activity of recombinant toxin was lower for lso as compared to llo. to understand the molecular basis of this difference, in particular with respect to the conserved trp-rich undecapeptide, a naturally occurring ala to phe substitution in lso was introduced into rllo. the rllo:a488f hemolysi ... | 2001 | 11583846 |
| detection of low levels of listeria monocytogenes within 20 hours using an evanescent wave biosensor. | 2001 | 11586938 | |
| inhibition of interleukin-12 production by auranofin, an anti-rheumatic gold compound, deviates cd4(+) t cells from the th1 to the th2 pathway. | 1. interleukin-12 (il-12) may play a central role in the development and progression of rheumatoid arthritis by driving the immune response towards t helper 1 (th1) type responses characterized by high ifn-gamma and low il-4 production. in this study we investigated the effect of auranofin (af), an anti-rheumatic gold compound, on il-12 production in mouse macrophages and dendritic cells, and studied whether af-mediated inhibition of il-12 production could regulate a cytokine profile of antigen ... | 2001 | 11588111 |
| intraoperative prostaglandin e1 improves antimicrobial and inflammatory responses in alveolar immune cells. | anesthesia and surgery decrease antimicrobial and increase proinflammatory functions of alveolar immune cells. thus, anti-inflammatory agents that do not further suppress antimicrobial functions are required. we tested the hypothesis that intraoperative prostaglandin e1 (pge1) suppresses proinflammatory responses and prevents the reduction in antimicrobial responses of alveolar immune cells. | 2001 | 11588457 |
| quantifying the relationship between multiple immunological parameters and host resistance: probing the limits of reductionism. | although reductionist experimental designs are excellent for identifying cells, molecules, or functions involved in resistance to particular microbes or cancer cells, they do not provide an integrated, quantitative view of immune function. in the present study, mice were treated with either dexamethasone (dex) or cyclosporin a (cya), and immune function and host resistance were evaluated. multivariate statistical methods were used to describe the relative importance of a broad range of immunolog ... | 2001 | 11591782 |
| the family of thiol-activated, cholesterol-binding cytolysins. | several species of both pathogenic and non-pathogenic grampositive bacteria within the genera streptococcus, clostridium and bacillus secrete cytolytic proteins that belong to a single, highly homologous family. the most widely known members of this family are streptolysin o, listeriolysin, perfringolysin, and pneumolysin. these toxins specifically require membrane cholesterol but, apparently, do not depend on any other specific cell surface receptor, so that they are able to lyse the cytoplasmi ... | 2001 | 11595631 |
| administration of superantigens protects mice from lethal listeria monocytogenes infection by enhancing cytotoxic t cells. | superantigens stimulate t-cell-receptor vbeta-selective t-cell proliferation accompanying the release of cytokines, which may eventually protect the host from microbial infections. we investigated here whether superantigens can rescue the host from lethal bacterial infection. mice were pretreated with staphylococcus aureus enterotoxin b (seb) 1 and 2 days before bacterial infection, and the mortality of infected mice was assessed. seb pretreatment protected mice from lethal infection with lister ... | 2001 | 11598032 |
| [listeria monocytogenes aortitis]. | 2001 | 11599169 | |
| effect of sampling method on the representative recovery of microorganisms from the surfaces of aquacultured finfish. | the objective of this study was to determine if a gentle rinse procedure was equivalent to the combination of excision and homogenization with a stomacher for the relative removal of various microorganisms from finfish fillets. fillets of hybrid striped bass and rainbow trout were obtained from local markets and sampled using three methods: rinse (r), excision followed by homogenization in a stomacher (s), and homogenization of fillets following a rinse (rs). microorganisms were enumerated on se ... | 2001 | 11601699 |
| comparative evaluation of culture- and bax polymerase chain reaction-based detection methods for listeria spp. and listeria monocytogenes in environmental and raw fish samples. | two commercial polymerase chain reaction (pcr)-based listeria detection systems, the bax for screening/listeria monocytogenes and the bax for screening/genus listeria, and a culture-based detection system, the biosynth l. monocytogenes detection system (lmds), were evaluated for their ability to detect l. monocytogenes and listeria spp. in raw ingredients and the processing environment. for detection of l. monocytogenes from raw fish, enrichment was performed in listeria enrichment broth (leb), ... | 2001 | 11601700 |
| efficacy of chitosan, carvacrol, and a hydrogen peroxide-based biocide against foodborne microorganisms in suspension and adhered to stainless steel. | the ability of natural compounds to inactivate foodborne organisms adhered to surfaces was investigated with the ultimate aim of replacing synthetic biocides by more environmentally friendly, natural alternatives. the antimicrobial efficacy of 0.5, 1.0, and 2.0% chitosan and spor-klenz rtu (a commercial biocide based on hydrogen peroxide and peroxyacetic acid) and 0.5, 1.25, and 2.0 mm carvacrol was determined at 20 degrees c against listeria monocytogenes, salmonella enterica serovar typhimuriu ... | 2001 | 11601703 |
| efficacy of trisodium phosphate solutions in reducing listeria monocytogenes populations on chicken skin during refrigerated storage. | chicken skin inoculated with l0(8) cfu/ml of listeria monocytogenes was dipped for 15 min in sterile water (control) and in 8, 10, or 12% trisodium phosphate (tsp) solutions. skin samples were stored at 2 degrees c for 5 days, with microbial monitoring on days 0, 1, 3, and 5 after treatment. compared to the water dip, all tsp treatments significantly (p < 0.05) reduced l monocytogenes populations on chicken skin. the concentration of the tsp was a significant factor in reducing the populations o ... | 2001 | 11601719 |
| a possible regulatory role for the metal-binding domain of cada, the listeria monocytogenes cd2+-atpase. | using the baculovirus/sf9 expression system, we produced cada and deltambd, a metal-binding domain, truncated cada. both proteins had the expected properties of p-type atpases: atp-induced cd2+ accumulation, cd2+-sensitive atp and pi phosphorylation and atpase activity. deltambd displayed lower initial transport velocity as well as lower maximal atpase activity than cada. mbd truncation flattened the cd2+ dependence of the atpase activity and increased apparent cd2+ affinity, suggesting a positi ... | 2001 | 11602255 |
| [emerging new risks of food-borne infections]. | 2001 | 11602389 | |
| inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. | antimicrobial activity of honey has been attributed to hydrogen peroxide, which is produced by naturally occurring glucose oxidase, and phenolic compounds, although lethality of and inhibition by these and other components against microorganisms vary greatly, depending on the floral source of nectar. this study was undertaken to compare honeys from six floral sources for their inhibitory activity against escherichia coli o157:h7, salmonella typhimurium, shigella sonnei, listeria monocytogenes, s ... | 2001 | 11603859 |
| random amplification of polymorphic dna typing of listeria monocytogenes isolated from meat. | to investigate the epidemiological characteristics of listeria monocytogenes isolated from imported or domestic meats, l. monocytogenes was isolated and identified through biochemical and serological tests, and epidemiological analysis of the isolates was carried out through the random amplification of polymorphic dna (rapd) method. fifty-four isolates were identified as l. monocytogenes through biochemical tests, of which 36 (67%) were confirmed as serotype 1, and 18 (33%) were serotype 4, thro ... | 2001 | 11603860 |
| transfer of eukaryotic expression plasmids to mammalian host cells by bacterial carriers. | the concept of transkingom transfer of dna from bacteria to other organisms has recently been extended to include eukaryotic host cells. intracellular bacteria have been shown to transfer eukaryotic expression plasmids to mammalian host cells in vitro and in vivo. this can be used to induce immune responses towards protein antigens encoded by the plasmid, to complement genetic defects or even to direct the production of proteins in appropriate organs. the ease of generating such vehicles makes t ... | 2001 | 11604322 |
| effects of vegetable type, package atmosphere and storage temperature on growth and survival of escherichia coli o157:h7 and listeria monocytogenes. | the survival and growth of escherichia coli o157:h7 (atcc 43888 and nctc 12900) and listeria monocytogenes (atcc 19114 and nctc 11994) during storage (4 and 8 degrees c) on ready-to-use (rtu) packaged vegetables (lettuce, swedes (rutabaga), dry coleslaw mix, soybean sprouts) were studied. the vegetables were sealed within oriented polypropylene packaging film, and modified atmospheres developed in packs during storage due to produce respiration. survival and growth patterns were dependent on veg ... | 2001 | 11641769 |
| epidural abscess and vertebral osteomyelitis caused by listeria monocytogenes: case report and literature review. | a 69-y-old male with an epidural abscess and concomitant vertebral osteomyelitis caused by listeria monocytogenes is presented, together with a brief review of the literature. to our knowledge, no cases of epidural abscess and only 3 cases of osteomyelitis have previously been reported in association with this organism. | 2001 | 11669234 |
| fulminant hepatitis b virus reactivation with concomitant listeriosis after fludarabine and rituximab therapy: case report. | chronic hepatitis b infection poses a problem in patients with malignancies undergoing chemotherapy. not uncommonly, hepatitis b virus (hbv) reactivates during or soon after chemotherapy. we report a case of a woman with acute prolymphocytic leukemia who received chemotherapy containing fludarabine and cyclophosphamide, followed by the cd 20 monoclonal antibody, rituximab. she developed fatal reactivation of hepatitis b with fulminant liver failure 3 months after completing treatment. listeria m ... | 2001 | 11669307 |
| virologic and immunologic parameters that predict clinical response of aids-associated kaposi's sarcoma to highly active antiretroviral therapy. | the purpose of the work was to assess the predictive value of biologic factors on the efficacy of highly active antiretroviral therapy alone or combined with chemotherapy on aids-associated kaposi's sarcoma. twenty-six aids-kaposi's sarcoma patients who started therapy with protease inhibitors were investigated. no baseline chemotherapy was associated with less severe initial clinical status. median follow-up was 652 d. the main outcome measures were as follows: best kaposi's sarcoma clinical re ... | 2001 | 11676823 |
| in vitro transcription of prfa-dependent and -independent genes of listeria monocytogenes. | in vitro transcription starting from the promoters of the listeria monocytogenes genes hly, plca, acta, mpl, prfa and iap has been studied. whereas transcription from phly, pplca and pacta is strictly prfa-dependent, that from piap, pprfa1/2 and, unexpectedly, also from pmpl is independent. initiation of in vitro transcription at all tested promoters except pprfa requires high concentrations of atp but not gtp. the nucleotides required in higher concentrations for efficient in vitro transcriptio ... | 2001 | 11679071 |
| pyrosequencing as a method for grouping of listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlb gene. | by using pyrosequencing (i.e., sequencing by synthesis) 106 strains of different serovars of listeria monocytogenes were rapidly grouped into four categories based on nucleotide variations at positions 1575 and 1578 of the inlb gene. strains of serovars 1/2a and 1/2c constituted one group, and strains of serovars 1/2b and 3b constituted another group, whereas serovar 4b strains were separated into two groups. | 2001 | 11679367 |
| comparative genomics of listeria species. | listeria monocytogenes is a food-borne pathogen with a high mortality rate that has also emerged as a paradigm for intracellular parasitism. we present and compare the genome sequences of l. monocytogenes (2,944,528 base pairs) and a nonpathogenic species, l. innocua (3,011,209 base pairs). we found a large number of predicted genes encoding surface and secreted proteins, transporters, and transcriptional regulators, consistent with the ability of both species to adapt to diverse environments. t ... | 2001 | 11679669 |
| listeria monocytogenes response regulators important for stress tolerance and pathogenesis. | environmental sensing by two-component signal transduction systems is likely to play a role for growth and survival of listeria monocytogenes both during transmission in food products and within a host organism. two-component systems typically consist of a membrane-associated sensor histidine kinase and a gene regulatory protein, the response regulator (rr). we have identified seven putative rr genes in l. monocytogenes lo28 by pcr using degenerate oligonucleotide primers. by insertional inactiv ... | 2001 | 11682188 |
| rapd analysis, serotyping, and esterase typing indicate that the population of listeria monocytogenes strains recovered from cheese and from patients with listeriosis in belgium are different. | populations of listeria monocytogenes strains isolated in belgium from cheese and from patients with listeriosis were characterised by randomly amplified polymorphic dna (rapd) analysis using two 10-mers primers (opa-04 and opa-13). high discrimination levels were obtained with each of these primers alone (discrimination indices (di) of 0.899 and 0.935 for opa13 and opa04, respectively) or in combination (di of 0.960). the clustering of strains obtained by rapd was compared with a clustering pre ... | 2001 | 11683471 |
| rapidly growing tumor-like brain lesion. | listeria monocytogenes accounts for 8-11% of the cases of bacterial meningitis which is associated with high mortality in patients with serious underlying diseases or those receiving immunosuppressive treatment. brain abscess due to l. monocytogenes is a very rare occurrence. the case reported here concerns a 54-year-old female patient with a rapidly growing tumor-like brain lesion. l. monocytogenes type 4b could be cultured from blood and brain biopsy. despite antimicrobial therapy with ampicil ... | 2001 | 11688907 |
| [spontaneous bacterial peritonitis caused by listeria monocytogenes in a patient with liver cirrhosis]. | 2001 | 11692785 | |
| activation of the arp2/3 complex by the listeria acta protein. acta binds two actin monomers and three subunits of the arp2/3 complex. | acta is a bacterially encoded protein that enables listeria monocytogenes to hijack the host cell actin cytoskeleton. it promotes arp2/3-dependent actin nucleation, but its interactions with cellular components of the nucleation machinery are not well understood. here we show that two domains of acta (residues 85-104 and 121-138) with sequence similarity to wasp homology 2 domains bind two actin monomers with submicromolar affinity. acta binds arp2/3 with a k(d) of 0.6 microm and competes for bi ... | 2001 | 11029465 |
| molecular ecology of tetracycline resistance: development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins. | phylogenetic analysis of tetracycline resistance genes encoding the ribosomal protection proteins (rpps) revealed the monophyletic origin of these genes. the most deeply branching class, exemplified by tet and otra, consisted of genes from the antibiotic-producing organisms streptomyces rimosus and streptomyces lividans. with a high degree of confidence, the corresponding genes of the other seven classes (tet m, tet s, tet o, tet w, tet q, tet t, and tetb p) formed phylogenetically distinct sepa ... | 2001 | 11133424 |
| molecular studies on the ecology of listeria monocytogenes in the smoked fish processing industry. | we have applied molecular approaches, including pcr-based detection strategies and dna fingerprinting methods, to study the ecology of listeria monocytogenes in food processing environments. a total of 531 samples, including raw fish, fish during the cold-smoking process, finished product, and environmental samples, were collected from three smoked fish processing facilities during five visits to each facility. a total of 95 (17.9%) of the samples tested positive for l. monocytogenes using a com ... | 2001 | 11133446 |
| listeria monocytogenes modulates macrophage cytokine responses through stat serine phosphorylation and the induction of suppressor of cytokine signaling 3. | macrophage activation as part of natural resistance to infection is caused by stimulation with ifn-gamma and by the invading microorganisms or microbial products. infection of macrophages with the gram-positive bacterium listeria monocytogenes for short periods before activation with ifn-gamma increased the phosphorylation of transcription factor stat1 at s727 and thereby the expression of ifn-gamma-induced genes. by contrast, persistent infection with viable bacteria or treatment with heat-kill ... | 2001 | 11123325 |
| role of macrophage scavenger receptors in response to listeria monocytogenes infection in mice. | type i and type ii macrophage scavenger receptors (sr-a i/ii) recognize a variety of polyanions including bacterial cell-wall products such as lipopolysaccharide, suggesting a role for sr-a i/ii in immunity against bacterial infection. sr-a i/ii-deficient (msr-a-/-) mice were more susceptible to infection with listeriolysin-o (llo)-producing listeria monocytogenes. after infection, kupffer cells in wild-type (msr-a+/+) mice phagocytized larger numbers of listeria than those in msr-a-/- mice. the ... | 2001 | 11141491 |
| bystander activation of cd8+ t cells contributes to the rapid production of ifn-gamma in response to bacterial pathogens. | the bacterium burkholderia pseudomallei causes a life-threatening disease called melioidosis. in vivo experiments in mice have identified that a rapid ifn-gamma response is essential for host survival. to identify the cellular sources of ifn-gamma, spleen cells from uninfected mice were stimulated with b. pseudomallei in vitro and assayed by elisa and flow cytometry. costaining for intracellular ifn-gamma vs cell surface markers demonstrated that nk cells and, more surprisingly, cd8(+) t cells w ... | 2001 | 11145690 |
| variable immunodominance hierarchies for h2-m3-restricted n-formyl peptides following bacterial infection. | h2-m3-restricted presentation of n-formyl methionine (f-met) peptides to cd8(+) t cells provides a mechanism for selective recognition of bacterial infection. in this report we demonstrate that listeria monocytogenes infection induces distinct cd8(+) t cell populations specific for each of the known listeria-derived formyl methionine peptides presented by m3. the sum h2-m3-restricted, listeria-specific t cell response constitutes a major fraction of the total cd8(+) t cell response to primary in ... | 2001 | 11145694 |
| characterization and pathogenic potential of listeria monocytogenes isolates from the smoked fish industry. | this study was designed to evaluate the hypothesis that some of the listeria monocytogenes subtypes associated with foods, specifically smoked fish, may have an attenuated ability to cause human disease. we tested this hypothesis by using two different approaches: (i) comparison of molecular subtypes found among 117 isolates from smoked fish, raw materials, fish in process, and processing environments with subtypes found among a collection of 275 human clinical isolates and (ii) the evaluation o ... | 2001 | 11157227 |
| characterization of recurrent and sporadic listeria monocytogenes isolates from raw milk and nondairy foods by pulsed-field gel electrophoresis, monocin typing, plasmid profiling, and cadmium and antibiotic resistance determination. | following previous surveys to assess the incidence of listeria monocytogenes in raw milk and nondairy foods processed in northern ireland, isolates were characterized as recurrent or sporadic on the basis of multilocus enzyme electrophoresis (mee) analysis and restriction fragment length polymorphism typing. in the present study, 45 representative recurrent and sporadic electrophoretic types (ets) previously identified by mee were subjected to pulsed-field gel electrophoresis (pfge) of genomic d ... | 2001 | 11157252 |
| a novel serotype-specific gene cassette (glta-gltb) is required for expression of teichoic acid-associated surface antigens in listeria monocytogenes of serotype 4b. | listeria monocytogenes serotype 4b strains account for about 40% of sporadic cases and many epidemics of listeriosis. mutations in a chromosomal locus resulted in loss of reactivity with all three monoclonal antibodies (mabs) which were specific to serotype 4b and the closely related serotypes 4d and 4e. here we show that this locus contains a serotype 4b-4d-4e-specific gene cassette (3,071 bp) which consists of two genes, glta and gltb, and is flanked by palindromic sequences (51 and 44 nucleot ... | 2001 | 11157924 |
| identification and molecular analysis of pcsb, a protein required for cell wall separation of group b streptococcus. | group b streptococcus (gbs) is the leading cause of bacterial sepsis and meningitis in neonates. n-terminal sequencing of major proteins in the culture supernatant of a clinical isolate of gbs identified a protein of about 50 kda which could be detected in all of 27 clinical isolates tested. the corresponding gene, designated pcsb, was isolated from a gbs cosmid library and subsequently sequenced. the deduced pcsb polypeptide consists of 447 amino acid residues (m(r), 46,754), carries a potentia ... | 2001 | 11157929 |
| purification and characterization of pcr-inhibitory components in blood cells. | in a recent study, immunoglobulin g in human plasma was identified as a major inhibitor of diagnostic pcr (w. abu al-soud, l. j. jönsson, and p. rådström. j. clin. microbiol. 38:345-350, 2000). in this study, two major pcr inhibitors in human blood cells were purified using size exclusion and anion-exchange chromatographic procedures. based on n-terminal amino acid sequencing and electrophoretic analysis of the purified polypeptides, hemoglobin and lactoferrin were identified as pcr-inhibitor co ... | 2001 | 11158094 |
| staphylococcus caprae strains carry determinants known to be involved in pathogenicity: a gene encoding an autolysin-binding fibronectin and the ica operon involved in biofilm formation. | the atlc gene (1,485 bp), encoding an autolysin which binds fibronectin, and the ica operon, involved in biofilm formation, were isolated from the chromosome of an infectious isolate of staphylococcus caprae and sequenced. atlc (155 kda) is similar to the staphylococcal autolysins atl, atle, aas (48 to 72% amino acid identity) and contains a putative signal peptide of 29 amino acids and two enzymatic centers (n-acetylmuramoyl-l-alanine amidase and endo-beta-n-acetylglucosaminidase) interconnecte ... | 2001 | 11159959 |
| human endothelial cell activation and mediator release in response to listeria monocytogenes virulence factors. | the interaction of listeria monocytogenes with endothelial cells represents a crucial step in the pathogenesis of listeriosis. incubation of human umbilical vein endothelial cells (huvec) with wild-type l. monocytogenes (egd) provoked immediate strong no synthesis, attributable to listerial presentation of listeriolysin o (llo), as the no release was missed upon employment of a deletion mutant for llo (egd hly mutant) and was reproduced by purified llo. studies of conditions lacking extracellula ... | 2001 | 11159983 |
| neural route of cerebral listeria monocytogenes murine infection: role of immune response mechanisms in controlling bacterial neuroinvasion. | the pathologic features of cerebral listeria monocytogenes infection strongly suggest that besides hematogenous spread, bacteria might also spread via a neural route. we propose that after snout infection of recombination activating gene 1 (rag-1)-deficient mice, l. monocytogenes spreads to the brain via a neural route. the neural route of invasion is suggested by (i) the immunostaining of l. monocytogenes in the trigeminal ganglia (tg) and brain stem but not in other areas of the brain; (ii) th ... | 2001 | 11160006 |
| conventional alpha beta t cells are sufficient for innate and adaptive immunity against enteric listeria monocytogenes. | we have begun to dissect the cellular requirements for generation of immunity against enteric infection by listeria monocytogenes using a novel t(-) b(-) nk(-) mouse strain (mice double deficient for the common cytokine receptor gamma-chain (gamma(c)) and the recombinase-activating gene-2 (rag2/gamma(c) mice). initial experiments showed that c57bl/6 mice and alymphoid rag2/gamma(c) mice had similar kinetics of bacterial accumulation in the spleen, liver, and brain early after intragastric l. mon ... | 2001 | 11160234 |
| a novel approach of direct ex vivo epitope mapping identifies dominant and subdominant cd4 and cd8 t cell epitopes from listeria monocytogenes. | we used a novel approach for the direct ex vivo identification and characterization of t cell epitopes based on the screening of peptide spot libraries with freshly isolated splenocytes in a sensitive enzyme-linked immunospot (elispot) assay. this technique was applied for the analysis of splenocytes from listeria monocytogenes-infected balb/c and c57bl/6 mice. the screening of peptide spot libraries covering the whole listeriolysin o and p60 of l. monocytogenes confirmed all known cd4 and cd8 t ... | 2001 | 11160235 |
| direct analysis of the dynamics of the intestinal mucosa cd8 t cell response to systemic virus infection. | the cd8 t cell response to vesicular stomatitis virus infection was characterized in the spleen and intestinal mucosa using mhc tetramers. surprisingly, the primary response persisted in the lamina propria long after the splenic response had declined. furthermore, the response was characterized by a protracted effector phase in which cytolytic activity in the lamina propria, but not in the spleen, was maintained. the appearance of ag-specific cells in the intestinal mucosa was largely, though no ... | 2001 | 11160292 |
| lipopolysaccharide-induced il-18 secretion from murine kupffer cells independently of myeloid differentiation factor 88 that is critically involved in induction of production of il-12 and il-1beta. | il-18, produced as biologically inactive precursor, is secreted from lps-stimulated macrophages after cleavage by caspase-1. in this study, we investigated the mechanism underlying caspase-1-mediated il-18 secretion. kupffer cells constantly stored il-18 and constitutively expressed caspase-1. inhibition of new protein synthesis only slightly reduced il-18 secretion, while it decreased and abrogated their il-1beta and il-12 secretion, respectively. kupffer cells deficient in toll-like receptor ( ... | 2001 | 11160328 |
| relationships between ifngamma, il-6, corticosterone, and listeria monocytogenes pathogenesis in balb/c mice. | the relationships between listeria monocytogenes (lm) pathogenesis, based on bacterial load, and serum levels of il-6, ifngamma, and corticosterone (cort) were quantified. serum ifngamma levels increased along with the lm burden; however, with lm burdens > or =3 x 10(6) cfu per spleen, the serum ifngamma level decreased along with a decrease in splenic weight. serum il-6 levels exponentially increased with increases of lm, and the cort level positively correlated with the increase in il-6 and lm ... | 2001 | 11161448 |
| actin filament nucleation by endosomes, lysosomes and secretory vesicles. | intracellular pathogens such as listeria monocytogenes and vaccinia virus propel themselves through the cytoplasm of mammalian cells by nucleating actin filaments. recently, actin assembly has also been shown to power the movement of intracellular vesicles, and this may be a mechanism underlying endomembrane movement in a variety of physiological contexts. surprisingly, class i myosins have been found to play important roles in both actin nucleation and endomembrane trafficking. | 2001 | 11163138 |
| evaluation of a recombinant listeria monocytogenes expressing an hiv protein that protects mice against viral challenge. | vaccine strategies that utilize cell mediated immunity to control infection will be a necessary component of human immunodeficiency virus (hiv) vaccines. in previous studies we have shown that a listeria monocytogenes recombinant expressing hiv-gag elicits strong cd8+ and cd4+ t cell responses against hiv gag in addition to its own secreted proteins. here, we show that lm-gag can protect mice against a viral challenge with a recombinant vaccinia virus expressing gag, in an antigen specific manne ... | 2001 | 11163666 |
| comparison of control of listeria by nitric oxide redox chemistry from murine macrophages and no donors: insights into listeriocidal activity of oxidative and nitrosative stress. | the physiological function of nitric oxide (no) in the defense against pathogens is multifaceted. the exact chemistry by which no combats intracellular pathogens such as listeria monocytogenes is yet unresolved. we examined the effects of no exposure, either delivered by no donors or generated in situ within ana-1 murine macrophages, on l. monocytogenes growth. production of no by the two nonoate compounds papa/no (nh2(c3h6)(n[n(o)no]c3h7) and dea/no (na(c2h5)2n[n(o)no]) resulted in l. monocytog ... | 2001 | 11165873 |
| strain-related differences of nonspecific respiratory defense mechanisms in rats using a pulmonary infectivity model. | a number of animal studies have assessed pulmonary host defense mechanisms by inoculating the lungs with the bacterial agent, listeria monocytogenes. most studies use only a single strain of the animal to be tested; however, strain-related differences in responsiveness to pulmonary toxicants have been well documented. it was the goal of this current investigation to measure the pulmonary defense responses of two different strains of rats in a lung infectivity model. fischer 344 (f344) and spragu ... | 2001 | 11153062 |
| the use of host cell machinery in the pathogenesis of listeria monocytogenes. | the bacterial pathogen, listeria monocytogenes, exploits the host cell's machinery, enabling the pathogen to enter into cells and spread from cell to cell. three bacterial surface proteins are crucial for these processes: internalin and inlb, which mediate entry into cells, and acta, which induces actin polymerisation at one pole of the bacterium and promotes intracellular and intercellular motility. recent studies have identified several of the cellular factors involved in the entry process and ... | 2001 | 11154924 |
| persistent infection with listeria monocytogenes in the kidney induces anti-inflammatory invariant fetal-type gammadelta t cells. | after intraperitoneal inoculation with listeria monocytogenes, gammadelta t cells appear in the peritoneal cavity preceding the appearance of alphabeta t cells. such gammadelta t cells predominantly express t-cell receptor (tcr)vgamma1/vdelta6, develop through an extrathymic pathway, and contribute to host defence against the bacteria. we have observed a gradual increase in gammadelta t cells in kidneys of mice after intrarenal inoculation with l. monocytogenes, which resulted in an unusually lo ... | 2001 | 11168642 |
| hijacking and exploitation of il-10 by intracellular pathogens. | macrophages play a central role in infections, as a target for pathogens and in activation of the immune system. interleukin-10 (il-10), a cytokine produced by macrophages, is a potent immunosuppressive factor. some intracellular pathogens specifically target macrophages for infection and use il-10 to dampen the host immune response and stall their elimination from the host. certain viruses induce production of cellular il-10 by macrophages, whereas other viruses encode their own viral il-10 hom ... | 2001 | 11173248 |
| listeria monocytogenes-infected phagocytes can initiate central nervous system infection in mice. | listeria monocytogenes-infected phagocytes are present in the bloodstream of experimentally infected mice, but whether they play a role in central nervous system (cns) invasion is unclear. to test whether bacteria within infected leukocytes could establish cns infection, experimentally infected mice were treated with gentamicin delivered by surgically implanted osmotic pumps. bacterial inhibitory titers in serum and plasma ranged from 1:16 to 1:256, and essentially all viable bacteria in the blo ... | 2001 | 11179297 |
| human lactoferrin and peptides derived from its n terminus are highly effective against infections with antibiotic-resistant bacteria. | since human lactoferrin (hlf) binds to bacterial products through its highly positively charged n terminus, we investigated which of the two cationic domains is involved in its bactericidal activity. the results revealed that hlf lacking the first three residues (hlf(-3n)) was less efficient than hlf in killing of antibiotic-resistant staphylococcus aureus, listeria monocytogenes, and klebsiella pneumoniae. both hlf preparations failed to kill escherichia coli o54. in addition, hlf(-3n) was less ... | 2001 | 11179314 |
| non-major histocompatibility complex control of antibody isotype and th1 versus th2 cytokines during experimental infection of mice with mycobacterium avium. | infection of different strains of mice with mycobacterium avium has revealed genetic control of the immunoglobulin isotype induced and of the balance between th1 and th2 cytokines. female balb/c or c57bl/10 mice were infected intranasally with 10(5) m. avium organisms. the antibody response was measured over 18 weeks by enzyme-linked immunosorbent assay and western blotting, while numbers of cytokine-producing cells were assessed at 12 to 15 weeks by elispot assay. upon infection, c57bl/10 mice ... | 2001 | 11179347 |
| aberrant macrophage and neutrophil population dynamics and impaired th1 response to listeria monocytogenes in colony-stimulating factor 1-deficient mice. | listeria monocytogenes, a facultative intracellular bacterium, has been used extensively to study innate immune responses. macrophages act as hosts for this bacterium as well as a major defense against it. using mice homozygous for a null mutation (csf1(op)) in the gene for the mononuclear phagocytic growth factor colony-stimulating factor 1 (csf-1), we have demonstrated that csf-1-regulated macrophages were essential to defend against a listerial infection. in the absence of csf-1, monocytes we ... | 2001 | 11179357 |
| interference between host resistance to listeria monocytogenes infection and ovalbumin-induced allergic responses in mice. | listeria monocytogenes promotes the induction of the t-helper 1 (th1) cell response, while ovalbumin (ova) induces a th2 cell response and allergic reactions, such as airway hyperreactivity and immunoglobulin e (ige) production. when mice were immunized with ova on day 7 after l. monocytogenes infection, eosinophilia in bronchoalveolar lavage and the production of total ige, ova-specific ige, interleukin-4 (il-4), and il-5 in the circulation were markedly suppressed. cytokine responses, includin ... | 2001 | 11179368 |
| from evil to good: a cytolysin in vaccine development. | current vaccination strategies mainly target antigens into the phagosomal, major histocompatibility complex class ii antigen-processing pathway and thus lead predominantly to humoral immune responses. the elicitation of cytotoxic t-cell responses instead requires introduction of antigens into the cytosol of professional antigen-presenting cells (apcs). the intracellular bacterium listeria monocytogenes gains access to the host cell cytosol by means of a cytolysin, listeriolysin o. vaccine resear ... | 2001 | 11166239 |
| use of live bacterial vaccine vectors for antigen delivery: potential and limitations. | most infectious agents are restricted to the mucosal membranes or their transit through the mucosa constitutes a critical step in the infection process. therefore, the elicitation of an efficient immune response, not only at systemic, but also at mucosal level, after vaccination is highly desirable, representing a significant advantage in order to prevent infection. this goal can be only achieved, when the vaccine formulation is administered by the mucosal route. however, soluble antigens given ... | 2001 | 11166877 |
| comparison of different enrichment broths and background flora for detection of heat-injured listeria monocytogenes in whole milk. | various primary enrichment broths, including university of vermont medium (uvm), listeria enrichment broth (leb), modified leb, and aerobic and anaerobic l-palcamy, were compared with aerobic and anaerobic pennsylvania state university (psu) broths for the detection of severely heat-injured (62.8 degrees c for 5, 10, or 15 min; no colony appearance after heat injury on aerobic trypticase soy agar containing 0.6% yeast extract and modified oxford medium) listeria monocytogenes scott a. anaerobic ... | 2001 | 11198438 |
| factors influencing resuscitation and growth of heat injured listeria monocytogenes 13-249 in sous vide cooked beef. | the growth of listeria monocytogenes 13-249 in vacuum-packed, minced beef was investigated as a function of degree of heat injury (including no injury i.e. uncooked beef), growth phase (logarithmic and late stationary phase), ph (5.6 and 6.2), and storage temperature (3, 10 and 20 degrees c) during a storage period of 30 days. late logarithmic and late stationary phase cultures of l. monocytogenes 13-249 showed similar growth in refrigerated, vacuum-packed, raw minced beef with a high ph (6.2). ... | 2001 | 11205945 |
| high incidence of listeria monocytogenes in european red smear cheese. | the incidence of listeria and listeria monocytogenes in european red smear cheese was determined in order to assess whether the lack of recent outbreaks of listeriosis associated with cheese is due to improved hygenic conditions in the dairies. out of european red-smear cheese samples of various types, 15.8% contained organisms of the genus listeria, 6.4% of the samples were contaminated with l. monocytogenes, 10.6% with l. innocua, and 1.2% with l. seeligeri. six cheese samples contained two or ... | 2001 | 11205958 |