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the my meal intake tool (m-mit): validity of a patient self- assessment for food and fluid intake at a single meal.hospital malnutrition is an under-recognized issue that leads to a variety of adverse outcomes, especially for older adults. food/fluid intake (ffi) monitoring in hospital can be used to identify those who are improving and those who need further treatment. current monitoring practices such as calorie counts are impractical for all patients and a patient-completed tool, if valid, could support routine ffi monitoring. the aim of this research was to determine whether the patient-completed my meal ...201829300419
larkinella knui sp. nov., isolated from soil.a gram-stain-negative, motile by gliding, rod-shaped, aerobic bacterium, designated 15j6-3t6t, was isolated from a soil sample collected from jeju island, south korea, and characterized taxonomically using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain 15j6-3t6t belongs to the family cytophagaceae and is related to larkinella harenae 15j9-9t (93.9 % similarity), larkinella arboricola z0532t (93.6 %), larkinella bovis m2tb15t (93.3 %), and larkinella inspera ...201829300161
planococcus salinus sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.a novel aerobic, gram-stain-positive, motile, moderately halophilic and coccoid bacterial strain, designated lcb217t, was isolated from a saline-alkali soil in north-western china and identified using a polyphasic taxonomic approach. growth occurred with 3-15 % (w/v) nacl (optimum 3-5 %), at 10-45 °c (optimum 30 °c) and at ph 7.0-9.0 (optimum ph 9.0). strain lcb217t contained mk-7 and mk-8 as the predominant menaquinones and anteiso-c15 : 0, iso-c14 : 0 and iso-c16 : 0 as the major fatty acids. ...201829300160
lactococcus reticulitermitis sp. nov., isolated from the gut of the subterranean termite reticulitermes speratus.strain rs-y01t was isolated from the gut of the wood-feeding subterranean termite reticulitermes speratus. phylogenetic analysis based on 16s rrna gene sequence indicated that the strain rs-y01t belonged to the genus lactococcus and was most closely related to lactococcus raffinolactis jcm 5706t with 98.1 % similarity in the 16s rrna gene, followed by lactococcus piscium jcm 16647t (97.2 %). genomic comparisons of strain rs-y01t with l. raffinolactis jcm 5706twere made using the genome-to-genome ...201829300157
bifidobacterium catulorum sp. nov., a novel taxon from the faeces of the baby common marmoset (callithrix jacchus).in our previous study based on hsp60 pcr-restriction fragment length polymorphism and 16s rrna gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus bifidobacterium. in that study, we also proposed that these isolated groups potentially represented novel species of the genus bifidobacterium. out of them, bifidobacterium aesculapii, bifidobacterium my ...201829300153
bacillus camelliae sp. nov., isolated from pu'er tea.a novel aerobic, gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1t, was isolated from ripened pu'er tea. based on 16s rrna gene sequence similarity comparisons, strain 7578-1t was grouped into the genus bacillus and appeared to be closely related to the type strains bacillus shackletoniilmg 18435t (98.4 %), bacillus acidicoladsm 14745t (97.6 %), bacillus paralicheniformis kacc 18426t (97.2 %) and bacillus ginsengihumi kctc 13944t (96.7 %). the fatty acid profile containing t ...201829300151
repurposing drugs for use against zika virus infection.zika virus (zikv) is a mosquito-borne flavivirus for which there are no vaccines or specific therapeutics. to find drugs active on the virus is a complex, expensive and time-consuming process. the prospect of drug repurposing, which consists of finding new indications for existing drugs, is an interesting alternative to expedite drug development for specific diseases. in theory, drug repurposing is also able to respond much more rapidly to a crisis than a classical drug discovery process. conseq ...201829299939
kurthia ruminicola sp. nov., isolated from the rumen contents of a holstein cow.gram-staining-positive, motile, rod-shaped bacteria, designated as h31022t and h31024 was isolated from rumen contents of a holstein cow. optimum growth occurred at 25°c and ph 7.0 on r2a agar medium. oxidase and catalase activities are positive. the 16s rrna gene sequence (1,452 bp) of the new isolates revealed they belong to the genus kurthia of the phylum firmicutes. highest gene sequence similarities were assessed to be with kurthia massiliensis jc30t (98.4%), kurthia senegalensis jc8et (97. ...201829299848
baekduia soli gen. nov., sp. nov., a novel bacterium isolated from the soil of baekdu mountain and proposal of a novel family name, baekduiaceae fam. nov.a taxonomic study was conducted on br7-21t, a bacterial strain isolated from the soil of a ginseng field in baekdu mountain. comparative studies of the 16s rrna gene sequence showed that the isolate was most closely related to conexibacter woesei dsm 14684t, solirubrobacter pauli atcc baa-492t, patulibacter minatonensis jcm 12834t, with 93.8%, 92.4%, and 91.5% sequence similarity, respectively; each genus represented a family in the order solirubrobacterales. strain br7-21t was gram-reaction pos ...201829299847
larkinella roseus sp. nov., a species of the family cytophagaceae isolated from beach soil.the taxonomic position of bacterial strain, designated 15j16-1t3at, recovered from a soil sample was established using a polyphasic approach. phylogenic analysis based on the 16s rrna gene sequence showed that strain 15j16-1t3at belonged to the family cytophagaceae, phylum bacteroidetes, and was most closely related to 'larkinella harenae' 15j9-9 (95.9% similarity), larkinella ripae 15j11-11t (95.6%), larkinella bovis m2tb15t (94.7%), larkinella arboricola z0532t (93.9%), and larkinella insperat ...201829299846
hiv-related healthcare needs and access barriers for brazilian transgender and gender diverse people.the aim of this study is to investigate hiv-related healthcare needs and access barriers for brazilian transgender and gender diverse people. data were collected from gender identity clinics and the questionnaire was also made available on the internet. out of the 543 participants-62.1% (n = 337) transgender women, 28.7 (n = 156) transgender men and 9.2% (n = 50) gender diverse people, 63.7% had been tested at least once in their lives. the prevalence of self-reported hiv-positive status among t ...201829299789
vibrio coralliirubri sp. nov., a new species isolated from mucus of red coral (corallium rubrum) collected at procida island, italy.strain corallo1t was isolated from mucus of red coral (corallium rubrum) at punta pizzaco (procida island, naples, italy). it was characterised as a gram-stain negative, motile, rod-shaped bacterium. strain corallo1t was found to show positive responses for cytochrome-c oxidase, catalase, reduction of nitrate and nitrite, β-galactosidase activity and hydrolysis of starch, xylan, peptone, tween 40, tween 80 and casein. strain corallo1t was found to be mesophilic, neutrophilic to alkalophilic and ...201829299771
a role for the endometrial microbiome in dysfunctional menstrual bleeding.this study aimed to characterise the microbial community within the endometrial cavity and endocervix in women with menorrhagia or dysmenorrhea. paired endocervical and endometrial biopsy samples were collected from women undergoing operative hysteroscopy and/or laparoscopy. samples were cohorted based on pathology, indications for surgery, and histological dating of the endometrium. samples were interrogated for the presence of microbial dna using a two-step next generation sequencing technolog ...201829299770
reliability and validity of preoperative mri for surgical decision making in chronic lateral ankle instability.to evaluate the value of analyzing the anterior talofibular ligament (atfl) on preoperative mri as a decision-making tool to determine the surgical technique in patients undergoing surgery for chronic lateral ankle instability.201829299765
cultivation of newly isolated microalgae coelastrum sp. in wastewater for simultaneous co2 fixation, lipid production and wastewater treatment.cultivation of microalgae in wastewater is a promising and cost-effective approach for both co2 biofixation and wastewater remediation. in this study, a new strain of coelastrum sp. was isolated from cattle manure leachate. the isolated microalgae were then cultivated in wastewater. effects of different scod concentrations (600, 750, 900, 1050 mg l-1) and light intensities (1000, 2300, 4600, 6900 and 10000 lux) on biomass production, co2 consumption rate and nutrient removal from wastewater were ...201829299676
bioprospection of culturable endophytic fungi associated with the ornamental plant pachystachys lutea.endophytes are fungi and bacteria that inhabit plant tissues without causing disease. endophytes have characteristics that are important for the health of the plant and have been isolated from several plants of economic and medicinal interest but rarely from ornamental plants. the current study isolates and identifies endophytic fungi from the leaves of pachystachys lutea and evaluates the antagonistic activity of these endophytes as well as cellulase production by the endophytes. fungi were iso ...201829299623
soft-pillared@magadiite: influence of the interlayer space and amine type on co2 adsorption.layered silicates are versatile materials that can be grafted with different organosilanes for several applications. despite this, there are few studies on the use of layered silicate-based materials in co2 adsorption. in this regard, the present study describes the synthesis of organo-magadiite followed by simultaneous grafting with two organosilanes ((3-glycidyloxypropyl)trimethoxysilane (gpts) and n1-(3-trimethoxysilylpropyl)diethylenetriamine (tmspeta)) to prepare an adsorbent labeled soft-p ...201829299587
isolation, derivative synthesis, and structure-activity relationships of antiparasitic bromopyrrole alkaloids from the marine sponge tedania brasiliensis.the isolation and identification of a series of new pseudoceratidine (1) derivatives from the sponge tedania brasiliensis enabled the evaluation of their antiparasitic activity against plasmodium falciparum, leishmania (leishmania) amazonensis, leishmania (leishmania) infantum, and trypanosoma cruzi, the causative agents of malaria, cutaneous leishmaniasis, visceral leishmaniasis, and chagas disease, respectively. the new 3-debromopseudoceratidine (4), 20-debromopseudoceratidine (5), 4-bromopseu ...201829297684
immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig.greater splanchnic nerve (gsn) is by far the largest of the splanchnic nerves and connects the paravertebral and prevertebral ganglia to transmit the majority of nociceptive information from the viscera. despite its importance, the immunohistochemical features of the porcine gsn neurons have not yet been examined. therefore, the aim of the study was to investigate the neurochemistry of the porcine gsn neurons and to compare their neurochemical coding with those of the paravertebral and preverteb ...201829297566
oral nodular lesions in patients with sjögren's syndrome: unusual oral implications of a systemic disorder.sjögren's syndrome (ss) is a systemic chronic autoimmune disorder affecting the lacrimal and salivary glands. ss may manifest as primary ss (pss) or secondary ss (sss), the latter occurring in the context of another autoimmune disorder. in both cases, the dry eyes and mouth affect the patient's quality of life. late complications may include blindness, dental tissue destruction, oral candidiasis and lymphoma. this paper reports two cases of ss, each of them presenting unusual oral nodular lesion ...201829297564
influence of root canal filling techniques on sealer penetration and bond strength to dentin.the influence of root canal filling techniques on the penetration of ah plus into dentinal tubules was evaluated using confocal laser scanning microscopy (clsm) and the impact on the bond strength of the filling material on the root dentin was evaluated using the push-out test. roots of the maxillary central incisors (n=40) were prepared with protaper universal and assigned to four groups: lateral condensation, vertical compaction, modified tagger hybrid and single cone. after root canal filling ...201829297560
an evaluation of the light output from 22 contemporary light curing units.this study measured the radiant power (mw), irradiance (mw/cm2) and emission spectra (mw/cm2/nm) of 22 new, or almost new, light curing units (lcus): - alt lux ii, biolux standard, bluephase g2, curing light xl 3000, demetron lc, dx turbo led 1200, ec450, ec500, emitter c, emitter d, kon-lux, led 3m espe, led lux ii, optilight color, optilight max, optilux 501, poly wireless, radii cal, radii plus, tl-01, valo cordless. these lcus were either monowave or multiple peak light emitting diode (led) ...201829297558
influence of the extended use of desensitizing toothpastes on dentin bonding, microhardness and roughness.the aim of this study was to evaluate the influence of an extended use of desensitizing toothpastes (dts) on dentin bonding, microhardness and roughness. one hundred and twenty bovine incisor teeth were randomly divided into four groups: g1, distilled water (water); g2, colgate total 12 (ct12); g3, colgate sensitive pro-relief (cspr); and g4, sensodyne repair ?αμπ; protect (srp). dentin surfaces were etched with 17% edta and 2 years of simulated tooth brushing (20,000 cycles) was performed on th ...201829297556
the in situ effect of titanium tetrafluoride gel on erosion/abrasion progression in human dentin.erosion incidence is increasing and its control is still a challenge in clinical practice. this study evaluated 4% tif4-gel effects on eroded human dentin subjected to in situ erosive/abrasive episodes. seventy-two previously eroded dentin slabs (0.05 m citric acid, ph 2.3, 20 min) were allocated to 6 groups (n=12) according to the treatment to be performed during the in situ phase and number of erosive/abrasive cycles, as follows: 4% tif4-gel applied once (tif41), twice (tif42) or three times ( ...201829297555
survival/success of dental implants with acid-etched surfaces: a retrospective evaluation after 8 to 10 years.the aim of this study was to conduct a retrospective evaluation of the survival and success rates of dental implants with acid-etched surfaces after 8-10 years of function. forty-four patients who received 183 implants 8-10 years ago were evaluated. clinical examinations were performed around the implants and natural teeth. the following parameters were measured: visible plaque index (vpi), marginal bleeding index (mbi), probing depth (pd), bleeding on probing (bop) and clinical attachment level ...201829297554
analyses of biofilm on implant abutment surfaces coating with diamond-like carbon and biocompatibility.the aim of this study was to evaluate the surface free energy (sfe), wetting and surface properties as well as antimicrobial, adhesion and biocompatibility properties of diamond-like carbon (dlc)-coated surfaces. in addition, the leakage of escherichia coli through the abutment-dental implant interface was also calculated. sfe was calculated from contact angle values; r a was measured before and after dlc coating. antimicrobial and adhesion properties against e. coli and cytotoxicity of dlc with ...201829297552
bioactive glass particles in two-dimensional and three-dimensional osteogenic cell cultures.this study aimed to investigate the influence of a three-dimensional cell culture model and bioactive glass (bg) particles on the expression of osteoblastic phenotypes in rat calvaria osteogenic cells culture. cells were seeded on two-dimensional (2d) and three-dimensional (3d) collagen with bg particles for up to 14 days. cell viability and alkaline phosphatase (alp) activity was performed. cell morphology and immunolabeling of noncollagenous bone matrix proteins were assessed by epifluorescenc ...201829297551
rank/rankl/opg expression in rapid maxillary expansion.the aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (rme) in rats. thirty male wistar rats were randomly assigned to two groups with 15 animals each: control (c) and rme group. rme was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. the animals were euthanized at 3, 7 and 10 days after rme. qrt-pcr was used to evaluate expression of tnfsf11 (rankl), tnfrsf11a (rank) and tnfrsf11b (opg). ...201829297549
repair of bone defects with chitosan-collagen biomembrane and scaffold containing calcium aluminate cement.innovative biomaterials can provide a promising new direction for the treatment of bone defects, stimulating a proper repair process, with no damage to adjacent tissues. the purpose of this in vivo study was to evaluate the biocompatibility and the osteoinductive capacity of chitosan-collagen biomembrane and scaffold containing calcium aluminate cement. eighteen new zealand white rabbits (oryctolagus cuniculus) were distributed according to the experimental times of analysis (7, 15 and 30 days). ...201829297548
genetic polymorphism in mmp9 may be associated with anterior open bite in children.anterior open bite (aob) has a multifactorial etiology caused by the interaction of sucking habits and genetic factors. the aim of this study was to evaluate the association between aob and polymorphisms in genes that encode matrix metalloproteinases (mmps) and their tissue inhibitors (timps). four hundred and seventy-two children that presented at least one sucking habit were evaluated. children were examined clinically for the presence of aob. genomic dna was extracted from saliva. genotyping ...201829297546
znwo4 nanocrystals: synthesis, morphology, photoluminescence and photocatalytic properties.the present joint experimental and theoretical work provides in-depth understanding on the morphology and structural, electronic, and optical properties of znwo4 nanocrystals. monoclinic znwo4 nanocrystals were prepared at three different temperatures (140, 150, and 160 °c) by a microwave hydrothermal method. then, the samples were investigated by x-ray diffraction with rietveld refinement analysis, field-emission scanning electron microscopy, transmission electronic microscopy, micro-raman and ...201829297523
tuberculosis among south american immigrants in são paulo municipality: an analysis in space and time.to identify spatial and temporal, and spatial variation in temporal trends in clusters of tuberculosis (tb) among south american migrants residing in são paulo municipality, sp, brazil, between 2006 and 2013.201829297430
randomised controlled trials for complex physiotherapy interventions are perfectly possible. 201829298753
ambient air pollution and thrombosis.air pollution is a growing public health concern of global significance. acute and chronic exposure is known to impair cardiovascular function, exacerbate disease and increase cardiovascular mortality. several plausible biological mechanisms have been proposed for these associations, however, at present, the pathways are incomplete. a seminal review by the american heart association (2010) concluded that the thrombotic effects of particulate air pollution likely contributed to their effects on c ...201829298690
the variome of pneumococcal virulence factors and regulators.in recent years, the idea of a highly immunogenic protein-based vaccine to combat streptococcus pneumoniae and its severe invasive infectious diseases has gained considerable interest. however, the target proteins to be included in a vaccine formulation have to accomplish several genetic and immunological characteristics, (such as conservation, distribution, immunogenicity and protective effect), in order to ensure its suitability and effectiveness. this study aimed to get comprehensive insights ...201829298677
highly sensitive avoidance plays a key role in sensory adaptation to deep-sea hydrothermal vent environments.the environments around deep-sea hydrothermal vents are very harsh conditions for organisms due to the possibility of exposure to highly toxic compounds and extremely hot venting there. despite such extreme environments, some indigenous species have thrived there. alvinellid worms (annelida) are among the organisms best adapted to high-temperature and oxidatively stressful venting regions. although intensive studies of the adaptation of these worms to the environments of hydrothermal vents have ...201829298328
evolution of morphology and locomotor performance in anurans: relationships with microhabitat diversification.the relationships between morphology, performance, behavior and ecology provide evidence for multiple and complex phenotypic adaptations. the anuran body plan, for example, is evolutionarily conserved and shows clear specializations to jumping performance back at least to the early jurassic. however, there are instances of more recent adaptation to habit diversity in the post-cranial skeleton, including relative limb length. the present study tested adaptive models of morphological evolution in ...201829297953
tessaracoccus terricola sp. nov., isolated from oil-contaminated soil.a gram-stain-positive, non-motile, yellow and rod-shaped actinobacterium, designated strain brt-at, isolated from oil-contaminated soil, grew at 15-40 °c, at ph 5.5-10.0 and at 0-2 % (w/v) nacl concentration. this strain was characterized by a polyphasic approach. the 16s rrna gene sequence analysis showed that strain brt-at belonged to the genus tessaracoccus and is closely related to tessaracoccus rhinocerotis yim 101269t, tessaracoccus flavescens sst-39t, tessaracoccus defluvii lnb-140t and t ...201829297849
ciceribacter azotifigens sp. nov., a nitrogen-fixing bacterium isolated from activated sludge.a gram-reaction-negative, catalase- and oxidase-positive, aerobic, transparent, motile and rod-shaped bacterium that was capable of fixing dinitrogen (designated strain a.slu09t), isolated from activated sludge, was characterized by a polyphasic approach to clarify its taxonomic position. strain a.slu09t was observed to grow optimally at 30 °c and at ph 7.0 on r2a agar medium. strain a.slu09t showed β-glucosidase activity, converting the major ginsenoside rd to ginsenoside f2. phylogenetic analy ...201829297848
marinirhabdus citrea sp. nov., a marine bacterium isolated from a seaweed.a gram-stain-negative, aerobic, rod-shaped (1.3-1.9×0.3-0.5 µm) and non-motile marine bacterium, designated mebic09412t, was isolated from seaweed collected at yeonggwang county, south korea. 16s rrna gene sequence analysis demonstrated that strain mebic09412t shared high sequence similarity with marinirhabdus gelatinilytica nh83t (95.4 %). growth was observed at 17-38 °c (optimum 30 °c), at ph 4.0-8.5 (optimum ph 7.0) and with 0.5-6.0 % (w/v; optimum 2.5 %) nacl. the predominant cellular fatty ...201829297847
bacillus tamaricis sp. nov., an alkaliphilic bacterium isolated from a tamarix cone soil.a gram-stain-positive, alkaliphilic bacterium, designated egi 80668t, was isolated from a tamarix cone soil in xinjiang, north-west china. cells were facultatively anaerobic, terminal endospore-forming and motile by means of peritrichous flagella. colonies were yellowish and the cells showed oxidase-negative and catalase-positive reactions. strain egi 80668t grew at ph 8.0-10.0 and with 0-10 % (w/v) nacl (optimally at ph 9.0 and with 1-2 % nacl) on marine agar 2216. the predominant menaquinone w ...201829297846
proteus columbae sp. nov., isolated from a pigeon in ma'anshan, china.a gram-negative, facultatively anaerobic bacillus, strain 08mas2615t, was isolated from the flesh of a pigeon specimen collected in ma'anshan, anhui province, china. phylogenetic analysis of 16s rrna gene sequences confirmed that strain 08mas2615t belonged to the genus proteus, and formed an independent branch which was clearly separated from the other six known species of proteus. strain 08mas2615t was more closely related to proteus vulgaris atcc 29905t and proteus penneri nctc 12737t than oth ...201829297845
nitrite-reducing ability is related to growth inhibition by nitrite in rhodobacter sphaeroides f. sp. denitrificans.growth inhibition of rhodobacter sphaeroides f. sp. denitrificans il106 by nitrite under anaerobic-light conditions became less pronounced when the gene encoding nitrite reductase was deleted. growth of another deletion mutant of the genes encoding nitric oxide reductase was severely suppressed by nitrite. our results suggest that nitrite reductase increases the sensitivity to nitrite through the production of nitric oxide.201829297256
beneficial protective role of endogenous lactic acid bacteria against mycotic contamination of honeybee beebread.the purpose of this article is to reveal the role of the lactic acid bacteria (lab) in the beebread transformation/preservation, biochemical properties of 25 honeybee endogenous lab strains, particularly: antifungal, proteolytic, and amylolytic activities putatively expressed in the beebread environment have been studied. seventeen fungal strains isolated from beebread samples were identified and checked for their ability to grow on simulated beebread substrate (sbs) and then used to study mycot ...201829297160
comparative evaluation of image quality among different detector configurations using area detector computed tomography.the 320-detector row computed tomography (ct) system, i.e., the area detector ct (adct), can perform helical scanning with detector configurations of 4-, 16-, 32-, 64-, 80-, 100-, and 160-detector rows for routine ct examinations. this phantom study aimed to compare the quality of images obtained using helical scan mode with different detector configurations. the image quality was measured using modulation transfer function (mtf) and noise power spectrum (nps). the system performance function (s ...201829297139
assessment of biomarkers in the neotropical fish brycon amazonicus exposed to cypermethrin-based insecticide.the effects of cypermethrin-based insecticide (cbi), commonly used in aquaculture and agriculture, were evaluated in matrinxa (brycon amazonicus) exposed to sub-lethal concentration (20% of lc50) for 96 h. physiological and biochemical effects were studied through biomarkers: lipid peroxidation (lpo), glutathione (gsh), and ascorbic acid concentrations; superoxide dismutase (sod), catalase (cat), glutathione peroxidase (gpx), and glucose-6-phosphate dehydrogenase (g6pdh) assays in the liver and ...201829297132
self-organized multi-layered graphene-boron-doped diamond hybrid nanowalls for high-performance electron emission devices.carbon nanomaterials such as nanotubes, nanoflakes/nanowalls, and graphene have been used as electron sources due to their superior field electron emission (fee) characteristics. however, these materials show poor stability and short lifetimes, which prevent their use in practical device applications. the aim of this study was to find an innovative nanomaterial possessing both high robustness and reliable fee behavior. herein, a hybrid structure of self-organized multi-layered graphene (mlg)-bor ...201829296984
retraction notice to "reversion of hepatic steatosis by exercise training in obese mice: the role of sterol regulatory element-binding protein-1c" [life sci. title 91/11-12 (2012) 395-401]. 201829295739
severe bronchopulmonary dysplasia - incidence and predictive factors in a prospective, multicenter study in very preterm infants with respiratory distress syndrome.severe bronchopulmonary dysplasia (bpd) remains a major complication of prematurity and can have significant impact on long-term pulmonary sequelae and adverse neurodevelopmental outcomes.201829295665
correction to: antifungal activity of myrtus communis against malassezia sp. isolated from the skin of patients with pityriasis versicolor.the original version of this article unfortunately contained two mistakes in authors' names.201829294237
thermoregulated phase-transition synthesis of two-dimensional carbon nanoplates rich in sp2 carbon and unimodal ultramicropores for kinetic gas separation.the development of highly selective, chemically stable and moisture-resistant adsorbents is a key milestone for gas separation. porous carbons featured with random orientation and cross-linking of turbostratic nanodomains usually have a wide distribution of micropores. here we have developed a thermoregulated phase-transition-assisted synthesis of carbon nanoplates with more than 80 % sp2 carbon, unimodal ultramicropore and a controllable thickness. the thin structure allows oriented growth of c ...201829292571
photophysical dynamics of the efficient emission and photosensitization of [ir(pqi)2(nn)]+ complexes.the photophysical dynamics of three complexes in the highly-emissive [ir(pqi)2(nn)]+ series were investigated aiming at unique photophysical features and applications in light-emitting and singlet oxygen sensitizing research fields. rational elucidation and franck-condon analyses of the observed emission spectra in nitrile solutions at 298 and 77 k reveal the true emissive nature of the lowest-lying triplet excited state (t1), consisting of a hybrid 3mlct/lcir(pqi)→pqi state. emissive deactivati ...201829292427
validity and accuracy of three radiographic dental age estimation methods in brazilians.to validate, analyse accuracy, and construct multiple regression formulae of three age estimation methods - liliequist and lundberg, haavikko, and mornstad - using mineralization stages of permanent teeth in brazilians.201829301112
changes in orchid populations and endophytic fungi with rainfall and prescribed burning in pterostylis revoluta in victoria, australia.wildfires are common in seasonally dry parts of the world with a mediterranean climate. prescribed burning is used to reduce fuel load and fire risk, but often without reliable information on its effects. this study investigated the effects of prescribed burns in different seasons on pterostylis revoluta, an autumn-flowering australian terrestrial orchid, and its orchid mycorrhizal fungi (omfs) to find the least damaging season for a prescribed burn.201829300863
multiplex real-time pcr using temperature sensitive primer-supplying hydrogel particles and its application for malaria species identification.real-time pcr, also called quantitative pcr (qpcr), has been powerful analytical tool for detection of nucleic acids since it developed. not only for biological research but also for diagnostic needs, qpcr technique requires capacity to detect multiple genes in recent years. solid phase pcr (sp-pcr) where one or two directional primers are immobilized on solid substrates could analyze multiplex genetic targets. however, conventional sp-pcr was subjected to restriction of application for lack of ...201829293604
sensitized aliphatic fluorination directed by terpenoidal enones: a "visible light" approach.in our continued effort to address the challenges of selective sp3 c-h fluorination on complex molecules, we report a sensitized aliphatic fluorination directed by terpenoidal enones using catalytic benzil and visible light (white leds). this sensitized approach is mild, simple to set up, and an economical alternative to our previous protocol based on direct excitation using uv light in a specialized apparatus. additionally, the amenability of this protocol to photochemical flow conditions and p ...201829293338
volatile compounds of argentinean honeys: correlation with floral and geographical origin.the determination of the botanical/geographical origin of honey provides assurance of the product's quality. in the present work, honeys from different ecoregions of argentina were analysed, and the possible link between the complete pollen profile of honey samples and their volatile composition was evaluated by multivariate statistical tools. a total of 110 volatile compounds were found and semiquantified in honey samples. redundancy analysis showed significant correlations between the volatile ...201829291855
evidence for the initial steps of dha biohydrogenation by mixed ruminal microorganisms from sheep involves formation of conjugated fatty acids.incubation of dha with sheep rumen fluid resulted in 80% disappearance in 6 h. the products were analyzed as their fatty acid (fa) methyl esters by gc-fid on sp-2560 and slb-il111 columns. the gc-online reduction × gc and gc-ms techniques demonstrated that all dha metabolites retained the c22 structure (no evidence of chain-shortening). two new transient dha products were identified: mono-trans methylene interrupted-dha and monoconjugated dha (mc-dha) isomers. identification of mc-dha was confir ...201829291262
occurrence of a novel tannase (tan blp ) in endophytic streptomyces sp. al1l from the leaf of ailanthus excelsa roxb.the tannase production ability by endophytic actinobacteria and the genetic identity of responsible tannase gene were determined. the studied strains were isolated from surface-sterilized leaf discs of ailanthus excelsa roxb. four strains were found to hydrolyze tannic acid on solid media containing 0.4% tannic acid. the strain al1l was found as tanblp indicating production of tannase with diverse of substrate affinity. the tannase production from the potential strain al1l was performed in liqui ...201829291146
precursor-directed combinatorial biosynthesis of cephalosporin analogue by endolithic actinobacterium streptomyces sp. al51 by utilizing thiophene derivative.natural products or their derivatives provide a reliable resource for new drugs. the multi-step chemical reaction to produce new drug is not only expensive but also release pollutants. the precursor-based combinatorial biosynthesis (pcb) is, however, a better option to produce novel natural products with potential pharmaceutical applications. the present work is an attempt to synthesize an antibacterial compound by transforming thiophene precursor using endolithic streptomyces sp. al51. the stre ...201829291144
evaluation of three serological tests for diagnosis of bovine brucellosis in smallholder farms in pakistan by estimating sensitivity and specificity using bayesian latent class analysis.the present study aimed to estimate the herd-level sensitivity (se) and specificity (sp) of three commonly used serological tests in naturally-infected cattle and buffalo in smallholder farms in pakistan. between february and june 2015, a cross-sectional study was carried out in five districts of punjab (kasur, okara, pakpattan, jhelum, and bhakkar) and two districts of sindh (badin and thatta). serum samples from mixed farms of cattle (n=441) and buffalo (n=621) were collected and tested using ...201829290297
design, synthesis, and antifungal activities of 3-acyl thiotetronic acid derivatives: new fatty acid synthase inhibitors.emerging fungal phytodiseases are increasingly becoming a food security threat. twenty-six new 3-acylthiotetronic acid derivatives were designed, synthesized, characterized, and evaluated for activities against valsa mali, curvularia lunata, fusarium graminearum, and fusarium oxysporum f. sp. lycopersici. among the 26 compounds, 6f was the most effective against v. mali, c. lunata, f. graminearum, and f. oxysporum f. sp. lycopersici with median effective concentrations (ec50) of 4.1, 3.1, 3.6, a ...201829290106
aerobic proteobacterial methylotrophs in movile cave: genomic and metagenomic analyses.movile cave (mangalia, romania) is a unique ecosystem where the food web is sustained by microbial primary production, analogous to deep-sea hydrothermal vents. specifically, chemoautotrophic microbes deriving energy from the oxidation of hydrogen sulphide and methane form the basis of the food web.201829291746
raoultella sp. sm1, a novel iron-reducing and uranium-precipitating strain.the main aim of this study was the characterisation of novel raoutella isolate, an iron-reducing and uranium-precipitating strain, originating from microbial mats occurring in the sediments of a closed down uranium mine in kowary (sw poland). characterisation was done in the context of its potential role in the functioning of these mats and the possibility to use them in uranium removal/recovery processes. in our experiment, we observed the biological precipitation of iron and uranium's secondar ...201829289017
development of microsatellite markers for myracrodruon urundeuva (f.f. & m.f. allemão), a highly endangered species from tropical forest based on next-generation sequencing.myracrodruon urundeuva is a tree species of high economic importance due the strength and durability of its wood. threatened of extinction in brazil, it is present only in a few forest remnants, mostly in conservation units. currently, there is little information on the genetic diversity of natural populations in brazil and even less information about the genome of this species. here, new species-specific microsatellite loci were developed based on next-generation sequencing (illumina). more tha ...201829288424
the rs243866/243865 polymorphisms in mmp-2 gene and the relationship with bp control in obese resistant hypertensive subjects.we sought to investigate whether the polymorphisms rs243865 (-1306c>t); rs243866 (-1575g>a) and rs2285053 (-735c>t) in metalloproteinases 2 - mmp-2 gene and rs17576 (q279r), rs17577 (q668r) and rs3918242 (-1562c>t) in mmp-9 gene are associated with clinical outcomes in obese resistant hypertensive (rh) subjects. one hundred and twenty rh were enrolled in this cross-sectional study and divided into obese (n=63) and non-obese (n=57) according to body mass index. genotypes were determined by real-t ...201829288728
newly generated cd4+ t cells acquire metabolic quiescence after thymic egress.mature naive t cells circulate through the secondary lymphoid organs in an actively enforced quiescent state. impaired cell survival and cell functions could be found when t cells have defects in quiescence. one of the key features of t cell quiescence is low basal metabolic activity. it remains unclear at which developmental stage t cells acquire this metabolic quiescence. we compared mitochondria among cd4 single-positive (sp) t cells in the thymus, cd4+ recent thymic emigrants (rtes), and mat ...201829288207
effect of n-terminal acetylation on lytic activity and lipid-packing perturbation induced in model membranes by a mastoparan-like peptide.l1a (idglkaiwkkvadllknt-nh2) is a peptide that displays a selective antibacterial activity to gram-negative bacteria without being hemolytic. its lytic activity in anionic lipid vesicles was strongly enhanced when its n-terminus was acetylated (ac-l1a). this modification seems to favor the perturbation of the lipid core of the bilayer by the peptide, resulting in higher membrane lysis. in the present study, we used lipid monolayers and bilayers as membrane model systems to explore the impact of ...201829287697
stabilizing effect of montmorillonite on acerola juice anthocyanins.this study was conducted to evaluate color and anthocyanin stability of clarified acerola juice (caj) as affected by montmorillonite (mnt) at different concentrations (0-6 wt%, dry basis). while non-complexed caj suffered noticeable color degradation with time and ph variations, the presence of mnt (especially at 4-6 wt%) not only changed the initial color of caj but also made it more stable with time and ph changes. caj/mnt mixtures were ultracentrifuged in order to separate them into supernata ...201829287466
polycyclic aromatic hydrocarbons content and fatty acids profile in coconut, safflower, evening primrose and linseed oils.this study aimed at evaluating the polycyclic aromatic hydrocarbons (pahs) contamination of commercial vegetable oils and examined the identity through the fatty acids profiles. coconut, safflower, evening primrose, and linseed oils marketed in são paulo (brazil) were investigated totaling 69 samples. four pahs, benzo[a]anthracene (baa), chrysene (chr), benzo[b]fluoranthene (bbf), and benzo[a]pyrene (bap), were detected in 96% of the samples at individual levels ranging from not detected to 14.9 ...201829287444
charge and hydrophobicity of casein peptides influence transepithelial transport and bioavailability.antioxidant casein peptides were separated by sp-sephadex c-25 and c 18 columns. the transepithelial transport and bioavailability including the transport ratio and the remaining ratios of antioxidant activity (rraa) of these peptide absorbates, were then investigated using a caco-2 cell monolayer. the results indicate that both the negatively charged peptide fractions (cf1, cf2 and cf3) and the more hydrophilic fraction (hf1) were mainly transported via pept1 and paracellular routes. the positi ...201829287421
simultaneous quantification of fluoxetine and norfluoxetine in colostrum and mature human milk using a 2-dimensional liquid chromatography-tandem mass spectrometry system.a two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2d lc-ms/ms) was employed for the determination of fluoxetine (flu) and norfluoxetine (n-flu) in colostrum and mature milk by direct sample injection. with a run time of 12 min representing a gain in throughput analysis, the validated methods furnished selectivity, extraction efficiency, accuracy, and precision in accordance with the criteria preconized by the european medicines agency guideline ...201829287263
addition of n-heterocyclic carbene catalyst to aryl esters induces remote c-si bond activation and benzylic carbon functionalization.through the incorporation of a silicon atom to an aryl carboxylic ester substrate, the resulting c-si bond can be activated via the addition of a carbene catalyst on a remote site. this strategy allows for efficient functionalization of the benzylic sp3-carbons of aryl carboxylic esters.201829286252
effects of resin architecture and protein size on nanoscale protein distribution in ion-exchange media.knowledge of the nanoscale distribution of proteins in chromatographic resins is critical to our mechanistic understanding of separations performance. however, the nano- to mesoscale architecture of these materials is challenging to characterize using conventional techniques. small-angle neutron scattering was used to probe (1) the nano- to mesoscale structure of chromatographic media and (2) protein sorption in these media in situ with protein-scale resolution. in particular, we characterize th ...201829286243
traceless silylation of β-c(sp3)-h bonds of alcohols via perfluorinated acetals.we report the silylation of primary c-h bonds located β to secondary and tertiary alcohols by exploiting perfluorinated esters as traceless directing groups. the conversion of a secondary or tertiary alcohol to a perfluoroalkyl ester and conversion of the ester to the corresponding silyl acetals by hydrosilylation allows for selective β-c(sp3)-h silylation catalyzed by the combination of [ir(cod)ome]2 and me4phen (3,4,7,8-tetramethyl-1,10-phenanthroline) to form 6-membered dioxasilinane. tamao-f ...201829283571
heterologous biosynthesis of nodulisporic acid f.nodulisporic acids comprise a group of valuable indole diterpenes that exhibit potent insecticidal activities. we report the identification of a gene cluster in the genome of the filamentous fungus hypoxylon pulicicidum (nodulisporium sp.) that contains genes responsible for the biosynthesis of nodulisporic acids. using penicillium paxilli as a heterologous host, and through pathway reconstitution experiments, we identified the function of four genes involved in the biosynthesis of the nodulispo ...201829283570
mechanism and structure of γ-resorcylate decarboxylase.γ-resorcylate decarboxylase (γ-rsd) has evolved to catalyze the reversible decarboxylation of 2,6-dihydroxybenzoate to resorcinol in a nonoxidative fashion. this enzyme is of significant interest because of its potential for the production of γ-resorcylate and other benzoic acid derivatives under environmentally sustainable conditions. kinetic constants for the decarboxylation of 2,6-dihydroxybenzoate catalyzed by γ-rsd from polaromonas sp. js666 are reported, and the enzyme is shown to be activ ...201829283551
infestation by ergasilus coatiarus (copepoda: ergasilidae) in two amazonian cichlids with new host record from peru: an ectoparasites natural control approach.the occurrence of copepods ergasilid was investigated in two species of cichlids of economic importance for aquaculture in the amazon region: cichla monoculus and chaetobranchus semifasciatus. the fish were collected from a semi-intensive fish farm, near the city of nauta, loreto state, peru. copepods were found in the gill filaments of 44 of 85 specimens (51.7%) of c. monoculus and in eight of 30 (26.6%) specimens of c. semifasciatus. the parasite was identified as ergasilus coatiarus based on ...201829288016
comparison of three different adenoidectomy techniques in children - has the conventional technique been surpassed?pharyngeal tonsil hyperplasia is the most frequent cause of nasal obstruction and chronic mouth breathing during childhood. adenoidectomy is the procedure of choice for the resolution of these symptoms. it is not yet known, however, whether the conventional technique ("blind curettage") has been surpassed by more modern adenoidectomy techniques (video-assisted, with the aid of instruments). this study aimed to compare the conventional adenoidectomy technique with two other emerging techniques, p ...201829287856
morphological and molecular characterization of a novel myxosporean parasite myxobolus bejeranoi n. sp. (cnidaria: myxosporea) from hybrid tilapia in israel.myxosporean infections can cause severe damage to commercially grown tilapia. here, we report a novel myxosporean that was found in gills of oreochromis aureus male × oreochromis niloticus female, which is an important aquaculture tilapia hybrid in israel. three-month-old fish were found to have cysts located in gill muscle tissue, which were filled with both immature and mature spores. affected fish displayed higher mortality rate. spore dimensions (10.8 ± 0.7 μm length × 6.8 ± 0.6 μm width) an ...201829285565
generation of diversity sets with high sp3 fraction using the photoredox coupling of organotrifluoroborates and organosilicates with heteroaryl/aryl bromides in continuous flow.the photoredox cross-coupling of aryl halides and potassium alkyl trifluoroborates is a very effective means to form csp3-csp2 bonds. however, this transformation is inefficient for the coupling of unactivated primary trifluoroborates. we have developed a generally useful, continuous flow csp3-csp2 coupling procedure for the synthesis of diverse product sets that is compatible with both trifluoroborates and silicate reagents. this universal protocol provides diversity sets from both primary and ...201829281285
sonographic evaluation of liver hemodynamic indices in overweight and obese dogs.hepatic circulatory disturbances have been associated with obesity and fatty liver in humans. in the veterinary literature, however, there is limited information regarding the effects of different body condition scores (bcs) on liver hemodynamic indices in dogs.201829280193
antimicrobial peptide k0-w6-hya1 induces stable structurally modified lipid domains in anionic membranes.considering the known different mode of action of antimicrobial peptides in zwitterionic and anionic cell membranes, the present work compares the action of the antimicrobial peptide k0-w6-hya1 (kifgaiwplalgalknlik-nh2) with zwitterionic and negatively charged model membranes, namely, liposomes composed of phosphatidylcholine (pc) and phosphatidylglycerol (pg) membranes, and a mixture of the two. differential scanning calorimetry (dsc), steady state fluorescence of the trp residue, dynamic light ...201829284086
antibacterial [2-(methacryloyloxy) ethyl] trimethylammonium chloride functionalized reduced graphene oxide/poly(ethylene-co-vinyl alcohol) multilayer barrier film for food packaging.the objective of present work was to construct antibacterial [2-(methacryloyloxy) ethyl] trimethylammonium chloride functionalized reduced graphene oxide/poly(ethylene-co-vinyl alcohol) (mtac-rgo/evoh) multilayer barrier films by using layer-by-layer assembly under a parallel electric field. besides barrier and mechanical properties, the antibacterial activities of the film and cytotoxicity of mtac-rgo nanosheets were extensively investigated. the functionalization of rgo was achieved by graftin ...201829281881
large-area self-assembly of silica microspheres/nanospheres by temperature-assisted dip-coating.this work reports a temperature-assisted dip-coating method for self-assembly of silica (sio2) microspheres/nanospheres (sps) as monolayers over large areas (∼cm2). the area over which self-assembled monolayers (sams) are formed can be controlled by tuning the suspension temperature (ts), which allows precise control over the meniscus shape. furthermore, the formation of periodic stripes of sams, with excellent dimensional control (stripe width and stripe-to-stripe spacing), is demonstrated usin ...201829280379
synthesis of 9,9-disubstituted fluorenes from 2-iodobiphenyls and α-diazoesters under palladium catalysis.9,9-disubstituted fluorenes are widely employed in materials science. we have developed a straightforward approach for the synthesis of 9,9-disubstituted fluorenes from 2-iodobiphenyls and α-diazoesters. the reaction proceeds via a tandem palladium-catalyzed c(sp2)-h activation/carbenoid insertion sequence.201829280375
diagnostic performance of ct signs for predicting adnexal torsion in women presenting with an adnexal mass and abdominal pain: a case-control study.to determine which ct findings or combinations of ct findings could accurately identify adnexal torsion in a cohort of women admitted for abdominal pain and presenting with an adnexal mass.201829279174
micro-ct in an ectopic pregnancy: new radiological and microscopical perspectives (and level) in the study of the fallopian tube. 201829279158
synergic in vitro combinations of artemisinin, pyrimethamine and methylene blue against neospora caninum.neospora caninum is a member of apicomplexa phylum, the causative agent of neosporosis. the neosporosis combat is not well established and several strategies related to vaccine, chemotherapy and immune modulation are under development. in this work, we evaluated the effects of artemisinin (art), methylene blue (mb) and pyrimethamine (pyr) alone or associated, on n. caninum proliferation and elimination using lacz tagged tachyzoites. the reactive oxygen species (ros) production after incubation w ...201829279093
bioinformatic approaches for comparative analysis of viruses.the field of viral genomic studies has experienced an unprecedented increase in data volume. new strains of known viruses are constantly being added to the genbank database and so are completely new species with little or no resemblance to our databases of sequences. in addition to this, metagenomic techniques have the potential to further increase the number and rate of sequenced genomes. besides, it is important to consider that viruses have a set of unique features that often break down molec ...201829277875
comparative metagenomics.thanks in large part to newer, better, and cheaper dna sequencing technologies, an enormous number of metagenomic sequence datasets have been and continue to be generated, covering a huge variety of environmental niches, including several different human body sites. comparing these metagenomes and identifying their commonalities and differences is a challenging task, due not only to the large amounts of data, but also because there are several methodological considerations that need to be taken ...201829277868
a practical guide for comparative genomics of mobile genetic elements in prokaryotic genomes.mobile genetic elements (mges) are an important feature of prokaryote genomes but are seldom well annotated and, consequently, are often underestimated. mges include transposons (tn), insertion sequences (iss), prophages, genomic islands (geis), integrons, and integrative and conjugative elements (ices). they are intimately involved in genome evolution and promote phenomena such as genomic expansion and rearrangement, emergence of virulence and pathogenicity, and symbiosis. in spite of the annot ...201829277867
phylogenomics.phylogenomics aims at reconstructing the evolutionary histories of organisms taking into account whole genomes or large fractions of genomes. the abundance of genomic data for an enormous variety of organisms has enabled phylogenomic inference of many groups, and this has motivated the development of many computer programs implementing the associated methods. this chapter surveys phylogenetic concepts and methods aimed at both gene tree and species tree reconstruction while also addressing commo ...201829277865
comparative genomics for prokaryotes.bacteria and archaea, collectively known as prokaryotes, have in general genomes that are much smaller than those of eukaryotes. as a result, thousands of these genomes have been sequenced. in prokaryotes, gene architecture lacks the intron-exon structure of eukaryotic genes (with an occasional exception). these two facts mean that there is an abundance of data for prokaryotic genomes, and that they are easier to study than the more complex eukaryotic genomes. in this chapter, we provide an over ...201829277863
gene phylogenies and orthologous groups.this chapter covers the theory and practice of ortholog gene set computation. in the theoretical part we give detailed and formal descriptions of the relevant concepts. we also cover the topic of graph-based clustering as a tool to compute ortholog gene sets. in the second part we provide an overview of practical considerations intended for researchers who need to determine orthologous genes from a collection of annotated genomes, briefly describing some of the most popular programs and resource ...201829277861
is there a role for functional mri for the assessment of extracapsular extension in prostate cancer?exctracapsular extension (ece) in prostate cancer has a high impact on treatment decision. mri might predict presence of ece non-invasively.201829277805
selective and simultaneous determination of indigo carmine and allura red in candy samples at the nano-concentration range by flow injection analysis with multiple pulse amperometric detection.a novel, unique electroanalytical method was developed for the simultaneous quantification of the dyes indigo carmine (ic) and allura red (ar) in candies by coupling flow injection analysis and multiple pulse amperometry with a cathodically pretreated boron-doped diamond electrode, using 0.30 mol l-1 h2so4 as supporting electrolyte. a dual-potential waveform was employed, causing the electrooxidation of either ic solely or ic and ar simultaneously. thence, subtraction of current signals was used ...201829277229
fate of nanoparticles during alum and ferric coagulation monitored using single particle icp-ms.in this study, aluminum sulfate, ferric sulfate, ferric chloride, and poly(diallyldimethylammonium chloride) (pdadmac) coagulation removal of citrate-stabilized silver and gold nanoparticles (nps) and uncoated titanium dioxide, cerium dioxide, and zinc oxide nps was investigated using a single particle (sp) icp-ms direct monitoring technique. zone 2 (charge neutralization) coagulation was performed in river water and more commonly used zone 4 (sweep floc) coagulation was performed in both river ...201829277033
metabolic and vascular pattern in medial pterygoid muscle is altered by chronic stress in an animal model of hypodontia.psychological stress is an important perpetuating, worsening and risk factor for temporomandibular disorders of muscular or articular origin. occlusion instability, by the way, is considered a risk factor of this pathology and can be reproduced in some experimental animal models. the exact physiologic mechanism underlying these relations however, remains unclear. our purpose was to test the hypothesis that chronic stress and unilateral exodontia induce metabolic and vascular changes in the media ...201829275100
wnt signaling perturbations underlie the genetic heterogeneity of robinow syndrome.locus heterogeneity characterizes a variety of skeletal dysplasias often due to interacting or overlapping signaling pathways. robinow syndrome is a skeletal disorder historically refractory to molecular diagnosis, potentially stemming from substantial genetic heterogeneity. all current known pathogenic variants reside in genes within the noncanonical wnt signaling pathway including ror2, wnt5a, and more recently, dvl1 and dvl3. however, ∼70% of autosomal-dominant robinow syndrome cases remain m ...201829276006
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