Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| thin-section ct findings of nontuberculous mycobacterial pulmonary diseases: comparison between mycobacterium avium-intracellulare complex and mycobacterium abscessus infection. | we aimed to compare the ct findings of nontuberculous mycobacterial pulmonary diseases caused by mycobacterium avium-intracellulare complex (mac) and mycobacterium abscessus. two chest radiologists analyzed retrospectively the thin-section ct findings of 51 patients with mac and 36 with m. abscessus infection in terms of patterns and forms of lung lesions. no significant difference was found between mac and m. abscessus infection in the presence of small nodules, tree-in-bud pattern, and bronchi ... | 2005 | 16224151 |
| mycobacterium abscessus infection after use of tumor necrosis factor alpha inhibitor therapy: case report and review of infectious complications associated with tumor necrosis factor alpha inhibitor use. | tumor necrosis factor alpha (tnf-alpha) inhibitors, such as infliximab and etanercept, are now frequently used in the treatment of inflammatory diseases including rheumatoid arthritis (ra) and crohn's disease. as an apparent result of their immune modulating activity, there has been an observed association between the use of these agents and the development of a wide range of infections, most notably mycobacterium tuberculosis. we describe a case of infection with mycobacterium abscessus in a 67 ... | 2005 | 16249064 |
| deep stromal mycobacterial keratitis: viable bacteria after six months of treatment: case report and literature review. | to report the presence of viable mycobacteria in a patient with keratitis treated for 6 months. species identification was performed using the pra method (polymerase chain reaction followed by restriction endonuclease analysis). clonality was evaluated with rapd (randomly amplified polymorphic dna) and eric-pcr (enterobacterial repetitive intergenic consensus-polymerase chain reaction) methods. the patient reported trauma due to a metallic foreign body 3 weeks prior to presentation. initial corn ... | 2005 | 16322845 |
| [two cases of pulmonary infection by mycobacterium abscessus in which drug susceptibility testing results conflicted with clinical courses]. | we encountered two cases of pulmonary infection by mycobacterium abscessus (m. abscessus). [case 1] a 66-year-old man who had been treated for non-tuberculous mycobacterium in the past was admitted because of productive cough. his chest x-ray film showed cavitation and direct microscopy of sputum revealed positive acid-fast bacilli (afb). he was given rifampicin (rfp), ethambutol (eb), and clarythromycin (cam), and then his symptoms and radiographic findings improved. [case 2] a 74-year-old man ... | 2005 | 15966372 |
| mycobacterium abscessus and other nontuberculous mycobacteria: evolving respiratory pathogens in cystic fibrosis: a case report and review. | cystic fibrosis (cf) is a risk factor for the development of nontuberculous mycobacteria (ntm) infection. prevalence of these organisms varies from center to center with the predominance of affected patients being in the adult population. the difficulty in diagnosing ntm infection in cf involves the overlap between signs and symptoms of underlying cf lung disease with its variable pathogens and the signs and symptoms attributable to pulmonary disease caused by ntm. bacterial overgrowth, especial ... | 2005 | 16004174 |
| in vitro activity of fluoroquinolones against mycobacterium abscessus and mycobacterium chelonae causing infectious keratitis after lasik in brazil. | to evaluate the in vitro activity of fluoroquinolones against mycobacterium abscessus and mycobacterium chelonae isolated from outbreaks of infectious keratitis in brazil. | 2005 | 16015094 |
| clinical, microbiological, and epidemiological findings of an outbreak of mycobacterium abscessus hand-and-foot disease. | in 2003, we identified an outbreak of clinically distinct lesions involving the hands and feet associated with a public wading pool in edmonton, alberta, canada. a total of 85 cases were identified. the management and follow-up of 41 children and 1 adult patients is presented. skin lesions occurred within a median incubation period of 29 days and approximately 88 days for the adult patient. lesions resolved within a median of 58 days and approximately 150 days for the adult patient. patients wer ... | 2005 | 16054324 |
| multicenter evaluation of mycobacteria identification by pcr restriction enzyme analysis in laboratories from latin america and the caribbean. | the identification of mycobacterial species in clinical isolates is essential for making patient care decisions. polymerase chain reaction (pcr) restriction enzyme analysis (pra) is a simple and rapid identification method, based on amplification of 441 bp of the hsp65 gene and restriction with bsteii and haeiii. as a contribution to the validation of pra, a multicenter study was performed in eight laboratories located in argentina, brazil, colombia, chile, and guadeloupe. each laboratory receiv ... | 2004 | 15722145 |
| a new method for species identification and differentiation of mycobacterium chelonae complex based on amplified hsp65 restriction analysis (ahspra). | members of the mycobacterium chelonae complex (mcc), namely m. chelonae, mycobacterium abscessus and mycobacterium immunogenum, have been implicated in nosocomial infections and occupational respiratory illnesses like hypersensitivity pneumonitis (hp) associated with contaminated metalworking fluid (mwf) exposures. close relationship among these member species makes their differentiation cumbersome using the existing methods. here we report a simple and rapid method for unambiguous identificatio ... | 2004 | 15680210 |
| pneumonectomy for nontuberculous mycobacterial infections. | pneumonectomy is considered in the treatment of nontuberculous mycobacterial infections when an entire lung is affected. however, this procedure carries high morbidity. we report on our experience in using pneumonectomy for treating patients with nontuberculous mycobacterial infections. | 2004 | 15276484 |
| late-onset mycobacterium abscessus sepsis in a very low birthweight premature infant: diagnostic and therapeutic challenges. | late-onset sepsis (after 3 days of life) is a frequent a complication found in very low birth weight (vlbw, 1000 to 1500 g) premature infants. we report the second case of late-onset sepsis caused by an uncommon pathogen, mycobacterium abscessus. | 2004 | 15311374 |
| mycobacterium abscessus as a cause of pacemaker infection. | mycobacterial infections of a pacemaker insertion site are very rare clinical events. such infections are caused primarily by staphylococci and streptococci and, less frequently, gram-negative organisms. | 2004 | 15448601 |
| [a case of bronchial ulcer due to infection by mycobacterium abscessus]. | we present a rare case of tracheobronchitis caused by mycobacterium abscessus. the patient was a 79-year-old man with a previous history of tuberculosis. for smear examinations, he repeatedly expectorated many acid-fast bacilli. bronchoscopic examination revealed the presence of ulceration on the lower end of the trachea and extending to the right main bronchus. mycobacterial cultures were used to grow mycobacterium abscessus. following an antimicrobial regimen of clarithromycin, amikacin, and c ... | 2004 | 15566008 |
| amoebal coculture of "mycobacterium massiliense" sp. nov. from the sputum of a patient with hemoptoic pneumonia. | a nonphotochromogenic, rapidly growing mycobacterium strain was isolated in pure culture from the sputum and the bronchoalveolar fluid of a patient with hemoptoic pneumonia by using axenic media and an amoebal coculture system. both isolates grew in less than 7 days at 24 to 37 degrees c with an optimal growth temperature of 30 degrees c. the isolates exhibited biochemical and antimicrobial susceptibility profiles overlapping those of mycobacterium abscessus, mycobacterium chelonae, and mycobact ... | 2004 | 15583272 |
| pulsed-field gel electrophoresis study of mycobacterium abscessus isolates previously affected by dna degradation. | dna degradation (which results in a smear pattern) occurs with almost 50% of mycobacterium abscessus strains during pulsed-field gel electrophoresis (pfge). we assessed the potential benefit of using thiourea-containing buffer with m. abscessus by studying 69 isolates not previously typeable by pfge (i.e., those with a smear pattern). random (epidemiologically unrelated) isolates that were typeable (no smear pattern) were included as controls. genomic dna was digested with drai, xbai, and asei. ... | 2004 | 15583285 |
| [a case of lung infection due to mycobacterium abscessus (m. abscessus) complicated with primary macroamylasemia]. | we report a case of lung infection due to mycobacterium abscessus (m. abscessus), complicated with primary macroamylasemia. a 76-year-old man was admitted to our hospital in august 2002 because of bloody sputum and an abnormal shadow found on chest radiography. the patient had had pulmonary tuberculosis from 1998 to 2000. he was found to be antacid bacillus-positive (gaffky 5) on examination of the sputum in august 2002, but after hospitalization was negative for tuberculosis bacillus on sputum ... | 2004 | 15228140 |
| performance assessment of a nested-pcr assay (the rapid bap-mtb) and the bd probetec et system for detection of mycobacterium tuberculosis in clinical specimens. | the performance of a nested pcr-based assay (the rapid bap-mtb; asiagen, taichung, taiwan) and the bd probetec et (dtb) system (becton dickinson, sparks, md.) for detection of mycobacterium tuberculosis was evaluated with 600 consecutive clinical samples. these samples, including 552 respiratory specimens and 48 nonrespiratory specimens, were collected from 333 patients treated at national taiwan university hospital from september to october 2003. the results of both assays were compared to the ... | 2004 | 15472315 |
| measurement of immunoglobulin g against mycobacterial antigen a60 in patients with cystic fibrosis and lung infection due to mycobacterium abscessus. | the diagnosis and prognosis of lung infections due to the emerging nontuberculous mycobacterium (ntm) mycobacterium abscessus are difficult to establish in children with cystic fibrosis. | 2004 | 15614693 |
| mycobacterium abscessus cellulitis and multifocal abscesses of the breasts in a transsexual from illicit intramammary injections of silicone. | we report the case of a 29-year-old transsexual who developed mycobacterium abscessus infection after receiving intramammary liquid silicone injections in the nonphysician office setting. our patient represents 1 of 14 confirmed and 11 suspected cases in new york city of m abscessus infection after illicit cosmetic procedures. as injectable cosmetic procedures are becoming increasingly popular, dermatologists should be aware of both the common and unusual complications. furthermore, all physicia ... | 2004 | 14988690 |
| discontinuation of secondary prophylaxis in aids patients with disseminated non-tuberculous mycobacteria infection. | from june 1994 to june 2002, disseminated infections due to non-tuberculous mycobacteria (ntm) were diagnosed in 42 of 716 (5.9%) non-haemophiliac hiv-infected patients aged > or =15 years who were treated at national taiwan university hospital. the median age of these patients was 35 years (range, 27 to 60 years). the median cd4+ cell count and plasma viral load at the time of diagnosis of disseminated ntm infections were 8 x 10(6)/l (range, 0 to 892 x 10(6)/l) and 37,600 copies/ml (range, <400 ... | 2004 | 15060688 |
| nontuberculous mycobacterial pulmonary infection in immunocompetent patients: comparison of thin-section ct and histopathologic findings. | to identify and describe the thin-section computed tomographic (ct) findings of nontuberculous mycobacterial (ntm) pulmonary infection in immunocompetent patients and to compare these findings with histopathologic findings. | 2004 | 15118112 |
| subcutaneous infection with mycobacterium abscessus in a renal transplant recipient. | transplant recipients show a high incidence of infections, often with atypical manifestations mainly because of drug-related immunosuppression. mycobacterial infections can be life-threatening in immunosuppressed patients due to the risk of dissemination. there have been only few reports of cutaneous infections with mycobacterium abscessus among immunosuppressed patients. we present a rare case with a sporotrichoid skin infection with m. abscessus in a renal recipient. the value of immunohistoch ... | 2004 | 15118383 |
| lightcycler-based differentiation of mycobacterium abscessus and mycobacterium chelonae. | in this study we introduce a rapid procedure to identify mycobacterium abscessus (types i and ii) and m. chelonae using lightcycler-based analysis of the hsp65 gene. results from 36 clinical strains were compared with hsp65 gene restriction analysis and biochemical profiles of bacilli. as all three methods yielded identical results for each isolate, this procedure offers an excellent alternative to previously established nucleic acid amplification-based techniques for the diagnosis of mycobacter ... | 2004 | 15243094 |
| reversal of linezolid-associated cytopenias, but not peripheral neuropathy, by administration of vitamin b6. | we sought to determine if vitamin b6 therapy would reverse linezolid-associated cytopenias and/or peripheral neuropathy. | 2004 | 15317746 |
| a novel mutation in ifn-gamma receptor 2 with dominant negative activity: biological consequences of homozygous and heterozygous states. | we identified two siblings homozygous for a single base pair deletion in the ifn-gammar2 transmembrane domain (791delg) who presented with multifocal mycobacterium abscessus osteomyelitis (patient 1) and disseminated cmv and mycobacterium avium complex infection (patient 2), respectively. although the patients showed no ifn-gammar activity, their healthy heterozygous parents showed only partial ifn-gammar activity. an hla-identical bone marrow transplant from the mother led patient 1 to complete ... | 2004 | 15356149 |
| microbiological identification in cystic fibrosis patients with cftr i1234v mutation. | recurrent and chronic bacterial pulmonary infection is the major cause of morbidity and mortality in cystic fibrosis (cf). over 6 months, 72 sputa or oropharyngeal samples were examined from 36 arab bedouin cf patients attending hamad general hospital, doha, qatar. more than 100 pathogens were isolated, mostly haemophilus influenzae, staphylococcus aureus and pseudomonas aeruginosa. unusual pathogens included stenotrophomonas maltophilia, acaligenes xylosoxidans and mycobacterium abscessus. it i ... | 2004 | 15357563 |
| rapidly growing mycobacterial infections after pedicures. | rapidly growing mycobacteria (rgm) can cause a variety of cutaneous and systemic diseases. the causative organisms are typically mycobacterium fortuitum or mycobacterium chelonae (also known as mycobacterium abscessus). primary cutaneous lesions may develop after a variable latent period, from weeks to several months, and usually result from direct inoculation after trauma, from injections, or during surgery via contaminated medical instruments. recently, investigators from the centers for disea ... | 2003 | 12756100 |
| radiographic and ct findings of nontuberculous mycobacterial pulmonary infection caused by mycobacterium abscessus. | objective: the purpose of our study was to describe the radiographic and ct findings in patients with pulmonary infections caused by mycobacterium abscessus, one of the more common rapidly growing nontuberculous mycobacteria that cause lung disease. conclusion: the main radiologic and ct manifestations of m. abscessus lung infection are bilateral small nodular opacities, bronchiectasis, and cavity formation. | 2003 | 12876037 |
| endophthalmitis caused by mycobacterium abscessus. | to describe the clinical and laboratory features of a case of endophthalmitis caused by mycobacterium abscessus in a patient immunocompetent. | 2003 | 14700104 |
| mycobacterium abscessus and children with cystic fibrosis. | we prospectively studied 298 patients with cystic fibrosis (mean age 11.3 years; range 2 months to 32 years; sex ratio, 0.47) for nontuberculous mycobacteria in respiratory samples from january 1, 1996, to december 31, 1999. mycobacterium abscessus was by far the most prevalent nontuberculous mycobacterium: 15 patients (6 male, 9 female; mean age 11.9 years; range 2.5-22 years) had at least one positive sample for this microorganism (versus 6 patients positive for m. avium complex), including 10 ... | 2003 | 14720400 |
| [a mycobacterium abscessus infection complicated with lipoid pneumonia]. | a 64-year-old man was admitted to our hospital because of productive cough and fever. chest radiography on admission revealed air space consolidation in the right middle and lower lung fields and ground-glass opacity in the left middle lung field. he had been constipated for several years and had taken mineral oil for about a year. sputum smears demonstrated acid bacilli, and cultures disclosed mycobacterium abscessus. the transbronchial lung biopsy specimen showed granulomatous inflammation and ... | 2003 | 12692999 |
| nontuberculous mycobacteria. i: multicenter prevalence study in cystic fibrosis. | nontuberculous mycobacteria (ntm) are potential respiratory pathogens in cystic fibrosis (cf). to assess the species-specific prevalence and risk factors for acquisition, we conducted a prospective, cross-sectional study of the prevalence of ntm and clinical features of patients at 21 u.s. centers. almost 10% of patients with cf who were 10 years or older were included (n = 986). the overall prevalence of ntm in sputum was 13.0% (range by center, 7-24%). mycobacterium avium complex (72%) and myc ... | 2003 | 12433668 |
| outbreak of infection with mycobacterium abscessus: is sodium dodecyl sulfate-polyacrylamide gel electrophoresis a useful technique for epidemiological typing? | 2003 | 12574318 | |
| [investigation of drug-resistance to rifampin and rpob gene sequence analysis of mycobacterium abscessus]. | to observe the resistance of mycobacterium abscessus to rifampin and to investigate if there is any mutation of rpob gene in strains with high minimal inhibitory concentration (mic). | 2003 | 14521758 |
| [nontuberculous mycobacterial keratitis: report of two cases causing infectious crystalline keratopathy]. | we report two cases of nontuberculous mycobacterial keratitis, occurring after corneal trauma with superficial foreign body and after perforating keratoplasty for alkali burn, respectively. patients initially presented with indolent white corneal infiltrates, which did not respond to topical treatment. both secondarily developed infectious crystalline keratopathy with unequal intensity. in the first case, the excised flap of lamellar keratectomy was cultured, allowing identification of mycobacte ... | 2003 | 12660593 |
| therapeutic keratectomy for mycobacterium abscessus keratitis after lasik. | we report successful treatment of a case of mycobacterium abscessus keratitis after laser in situ keratomileusis (lasik) with therapeutic lamellar keratectomy. a 34-year-old woman developed a 2 x 2 mm feathery infiltration within the interface inferior to the pupil margin with mild inflammation of the conjunctiva in her left eye 40 days after lasik surgery. bacterial culture from the infiltrates of the interface of the stromal bed revealed mycobacterium abscessus. after combination antibiotic th ... | 2003 | 14724729 |
| outbreak of mycobacterium abscessus infection after soft tissue augmentation. | illicit soft-tissue augmentation performed in new york city resulted in an outbreak of mycobacterium abscessus infection in 2002. | 2003 | 12930343 |
| [a case of lung infection due to mycobacterium abscessus]. | we report a case of lung infection due to mycobacterium abscessus (m. abscessus). a 60 year-old woman was admitted to our hospital because of an abnormal shadow found on chest radiography and a bloody sputum. chest radiography revealed infiltrative and linear shadows in the middle and lower fields of both lungs. the sputum smears were negative for acid-fast bacilli, but sputum culture was positive. rapidly growing mycobacteria were detected. the patient was treated with clarithromycin. since m. ... | 2003 | 14503341 |
| [activity of cephems and carbapenems against clinically isolated mycobacterium abscessus]. | to screen effective useful drugs for disease due to m. abscessus, we determined mic of 3 cephems [ceftazidime (caz), cefoxitin (cfx), flomoxef (fmox)] and 3 carbapenems [imipenem (ipm), panipenem (papm), meropenem (mepm)] for 8 strains of clinically isolated m. abscessus by micro-dilution method using mgit system. in all the 8 strains, mics of caz are higher than 32 micrograms/ml. mic50, mic90, mic range of cfx are 32 micrograms/ml, > 32 micrograms/ml and 16- > 32 micrograms/ml respectively, and ... | 2003 | 14577345 |
| identifying mycobacterium species and strain typing using a microfluidic labchip instrument. | we developed schemes for rapid identification of mycobacterium species and strain typing using a microfluidic labchip instrument. a 439-bp region of the gene that codes for the 65-kda heat shock protein (hsp65), which has sequence polymorphisms specific for most mycobacterial species, was examined using pcr-restriction analysis (pra). we performed pra in duplicate, using 2 strains each of 12 species, and observed that fragment sizes (bp) determined automatically by the instrument were consistent ... | 2003 | 14579744 |
| foreign-body reaction with mycobacterium abseccus superinfection. | mycobacterium abscessus is a rare cause of skin and soft tissue infections that often results from inoculation with contaminated foreign material. a 41-year-old woman is described regarding an outbreak of m. abscessus following soft tissue augmentation. clinical features and treatment options are reviewed. | 2003 | 14594602 |
| forty years of disinfectant failure: outbreak of postinjection mycobacterium abscessus infection caused by contamination of benzalkonium chloride. | benzalkonium chloride (bc) continues to be used as an antiseptic and contributes to serious outbreaks of disease. in july 1999, 6 postinjection joint infections caused by mycobacterium abscessus were reported to the texas department of health (austin). we investigated this outbreak and identified 12 case patients who had been seen by the same physician and who had received an intra-articular or periarticular steroid injection during the period of 1 april through 31 july 1999. m. abscessus was cu ... | 2003 | 12684906 |
| effects of ortho-phthalaldehyde, glutaraldehyde and chlorhexidine diacetate on mycobacterium chelonae and mycobacterium abscessus strains with modified permeability. | the mechanisms of the mycobactericidal action of ortho-phthalaldehyde (opa), glutaraldehyde (gta) and chlorhexidine diacetate (cha) were investigated using mycobacterial spheroplasts of two reference strains, mycobacterium chelonae nctc 946, mycobacterium abscessus nctc 10882 and two gta-resistant strains, m. chelonae epping and m. chelonae harefield. transmission electron microscopy of the spheroplasts revealed an altered cell wall structure compared with the parent cells. structural alteration ... | 2003 | 12615857 |
| rpob-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. | nonpigmented and late-pigmenting rapidly growing mycobacteria (rgm) are increasingly isolated in clinical microbiology laboratories. their accurate identification remains problematic because classification is labor intensive work and because new taxa are not often incorporated into classification databases. also, 16s rrna gene sequence analysis underestimates rgm diversity and does not distinguish between all taxa. we determined the complete nucleotide sequence of the rpob gene, which encodes th ... | 2003 | 14662964 |
| the changing pattern of nontuberculous mycobacterial disease. | nontuberculous mycobacteria are human opportunistic pathogens whose source of infection is the environment. these include both slow-growing (eg, mycobacterium kansasii and mycobacterium avium) and rapid-growing (eg, mycobacterium abscessus and mycobacterium fortuitum) species. transmission is through ingestion or inhalation of water, particulate matter or aerosols, or through trauma. the historic presentation of pulmonary disease in older individuals with predisposing lung conditions and in chil ... | 2003 | 18159470 |
| bilateral mycobacterium abscessus keratitis after laser in situ keratomileusis. | a 35-year-old man was diagnosed with mycobacterium abscessus keratitis in the left eye 3 weeks after bilateral laser in situ keratomileusis (lasik). infection in the right eye developed 6 weeks after surgery. despite aggressive treatment with topical amikacin and clarithromycin and oral clarithromycin, the infection progressed in both eyes. to improve antibiotic penetration, the lasik flap was removed in both eyes. culture positivity was prolonged; however, after 8 weeks of intensive topical ant ... | 2002 | 11978473 |
| [mycobacterium abscessus infection complicated with diabetes mellitus]. | we report a case of mycobacterium abscessus infection complicated with diabetes mellitus. a 38-year-old man with diabetes mellitus as an underlying disease, was admitted to our hospital because of a productive cough. he had had pulmonary tuberculosis two years before. chest radiography revealed infiltration in both lung apices and chest ct showed a cavitary lesion in the left upper lobe. gaffky 2 was found on a sputum smear. however, in the examination of pcr on sputum, not only m. tuberculosis ... | 2002 | 11925921 |
| a polymorphic region in mycobacterium abscessus contains a novel insertion sequence element. | a polymorphic region was discovered in the genetically uncharacterized opportunistic pathogen mycobacterium abscessus. the region contains a novel 1.7 kb insertion sequence (is) named ismab1. ismab1 contains two complete orfs and one partial orf located in segments with over 80% nucleotide identity to mycobacterium avium is1601 and is999 and to previously unreported is-like elements from mycobacterium smegmatis. the marked similarity within this family of elements is supportive of horizontal tra ... | 2002 | 12368432 |
| mycobacterium abscessus empyema in a lung transplant recipient. | non-tuberculous mycobacteria (ntm) have emerged as important pathogens in organ transplant recipients. because ntm pulmonary infections vary in their clinical and radiographic presentations, heightened clinical suspicion is necessary for accurate diagnosis. we report a case of mycobacterium abscessus empyema in a lung transplant recipient. repeated attempts at identifying the organism from a variety of clinical specimens led to the correct diagnosis and treatment. | 2002 | 11897529 |
| successful treatment with faropenem and clarithromycin of pulmonary mycobacterium abscessus infection. | mycobacterium abscessus accounts for 80% of rapidly growing mycobacterial pulmonary infections and can be lethal. treatment is difficult because of the paucity of effective drugs. we describe a patient with pulmonary m. abscessus infection who was treated with a regimen that included faropenem, a novel oral penem, and clarithromycin. he showed favorable responses to the treatment for more than 12 months. in vitro, faropenem had considerable inhibitory activities against 56 strains of rapidly gro ... | 2002 | 12373490 |
| evaluation of a commercial line probe assay for identification of mycobacterium species from liquid and solid culture. | the performance of a commercial line probe assay (lipa) (inno-lipa mycobacteria; innogenetics, belgium) for the detection and identification of mycobacterium species from liquid and solid culture was evaluated at five routine clinical laboratories. the lipa method is based on the reverse hybridization principle, in which the mycobacterial 16s-23s ribosomal rna (rrna) spacer region is amplified by polymerase chain reaction (pcr). amplicons are subsequently hybridized with oligonucleotide probes a ... | 2002 | 12461589 |
| large-scale outbreak of infection with mycobacterium chelonae subsp. abscessus after penicillin injection. | an outbreak of infection with mycobacterium chelonae subsp. abscessus after the injection of penicillin in 86 patients attending a factory hospital is reported. the bacterium was isolated both from lids and from the soil where the drug was stored. molecular analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and plasmids revealed a pattern identical to that of the strains isolated from the wounds. the source of the infections was soil contamination of the ... | 2002 | 12089291 |
| comparison of the in vitro activity of the glycylcycline tigecycline (formerly gar-936) with those of tetracycline, minocycline, and doxycycline against isolates of nontuberculous mycobacteria. | we compared the in vitro activity of the glycylcycline tigecycline (formerly gar-936) with those of tetracycline, doxycycline, and minocycline by broth microdilution against 76 isolates belonging to seven species of rapidly growing mycobacteria (rgm) and 45 isolates belonging to five species of slowly growing nontuberculous mycobacteria (ntm). by using a resistance breakpoint of >4 micro g/ml for tigecycline and >8 micro g/ml for tetracycline, all rgm were highly susceptible to tigecycline, with ... | 2002 | 12234839 |
| pulmonary disease caused by rapidly growing mycobacteria. | the rapidly growing mycobacteria (rgm) differ from slow-growing mycobacteria such as mycobacterium tuberculosis by virtue of their more rapid growth in culture media and their in vitro resistance to standard antituberculosis drugs. the rgm can produce numerous infections including chronic lung disease. the most common causes of pulmonary disease are mycobacterium abscessus and mycobacterium fortuitum. this article reviews the management of patients with lung disease caused by rgm. | 2002 | 12370998 |
| [recurrent otitis media and mastoiditis due to atypical mycobacteria]. | an eleven-year-old girl was operated on due to right-sided chronic otitis media with effusion. after three months, an impressive enlargement of the mucosal lining developed, for which thorough debridement of the middle ear and mastoid was performed. histological examination revealed a granulomatous inflammation, with negative ziehl-neelsen staining. standard bacteriological cultures revealed no pathogenic micro-organisms. three weeks later the same clinical picture developed, once again followed ... | 2002 | 11925802 |
| fatal pulmonary infection due to multidrug-resistant mycobacterium abscessus in a patient with cystic fibrosis. | we report a case of fatal pulmonary infection caused by mycobacterium abscessus in a young patient with cystic fibrosis, who underwent bipulmonary transplantation after a 1-year history of severe lung disease. fifteen days after surgery he developed septic fever with progressive deterioration in lung function. m. abscessus, initially isolated from a pleural fluid specimen, was then recovered from repeated blood samples, suggesting a disseminated nature of the mycobacterial disease. drug suscepti ... | 2001 | 11158161 |
| neurologic manifestations of disseminated infection with mycobacterium abscessus. | mycobacterium abscessus is a ubiquitous, saprophytic organism with low pathogenic potential. the authors describe the previously unreported clinical features of meningitis and native valve endocarditis caused by this rapidly growing atypical mycobacterium. the fatal outcome of this unusual case coincides with the grim prognosis of this disseminated infection and the significant mortality rate associated with neurologic complications of infective endocarditis. | 2001 | 11274327 |
| lack of transmission of mycobacterium abscessus among patients with cystic fibrosis attending a single clinic. | we retrospectively analyzed 1062 respiratory specimens from 214 patients with cystic fibrosis, of whom 5 patients had 36 cultures positive for m. abscessus. results of molecular typing demonstrated that each of these 5 patients carried a single unique strain (genotype), which suggests that it may not be necessary to segregate patients with cf who are colonized or infected with m. abscessus from those who are not. | 2001 | 11340540 |
| clarithromycin resistance to mycobacterium abscessus. | 2001 | 11446360 | |
| failure of treatment for chronic mycobacterium abscessus meningitis despite adequate clarithromycin levels in cerebrospinal fluid. | we report a case of posttraumatic meningitis due to mycobacterium abscessus, treated initially with oral clarithromycin and intravenous amikacin plus intrathecal amikacin. despite cerebrospinal fluid (csf) levels of clarithromycin and amikacin in excess of their in vitro minimum inhibitory concentrations for the organism, the csf cultures remained continuously positive for m. abscessus. to our knowledge, this is the first documented case of m. abscessus meningitis and the first report of measure ... | 2001 | 11486298 |
| disseminated mycobacterium abscessus infection manifesting as fever of unknown origin and intra-abdominal lymphadenitis: case report and literature review. | mycobacterium abscessus is a rapidly growing mycobacterium found in soil and water throughout the world. disease in immunocompetent patients usually consists of localized skin and soft tissue infections. in contrast, disseminated disease is uncommon, usually presents with rash, and almost always occurs in an immunocompromised host. we describe an unusual case of disseminated m. abscessus infection manifesting as fever of unknown origin and intra-abdominal lymphadenitis, but without rash. our pat ... | 2001 | 11173189 |
| mycobacterium abscessus wound infection. | we report an isolated case of wound infection due to mycobacterium abscessus following minor cutaneous surgery. the patient had routine skin cancer surgery in a private dermatology practice setting. he presented 2 weeks later with a wound infection which failed to respond to cephalexin. the patient reported that he had walked through salt water and bushes with exposed surgical wounds 1 week postoperatively. tissue cultures later grew m. abscessus. the patient was successfully treated with oral c ... | 2001 | 11233716 |
| mycobacterium immunogenum sp. nov., a novel species related to mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy. | pcr-restriction enzyme pattern analysis of a 439 bp hsp65 gene segment identified 113 unique isolates among non-pigmented rapidly growing mycobacteria (rgm) from clinical and environmental sources that failed to match currently recognized species patterns. this group represented 40% of isolates recovered from bronchoscope contamination pseudo-outbreaks, 0% of disease-associated nosocomial outbreaks and 4% of routine clinical isolates of the mycobacterium abscessus/mycobacterium chelonae group su ... | 2001 | 11594606 |
| orbital lipogranulomatous inflammation harboring mycobacterium abscessus. | a case of iatrogenic mycobacterium abscessus orbital infection is presented to call attention to its distinct histopathologic appearance, mechanism of organism, virulence, and clinical management. | 2001 | 11642495 |
| comparison of methods for identification of mycobacterium abscessus and m. chelonae isolates. | mycobacterium abscessus and mycobacterium chelonae are two closely related species that are often not distinguished by clinical laboratories despite the fact they cause diseases requiring different treatment regimens. multilocus enzyme electrophoresis, pcr-restriction fragment length polymorphism analysis of the 65-kda heat shock protein gene, biochemical tests, and high-performance liquid chromatography of mycolic acids were used to identify 75 isolates as either m. abscessus or m. chelonae tha ... | 2001 | 11682537 |
| mycobacterium abscessus: a cutaneous infection in a patient on renal replacement therapy. | we report a 72-year-old man on haemodialysis who presented with multiple abscesses on his lower legs. routine bacterial culture of abscess pus was reported as 'sterile' after 48 h, leading to the suspicion of a mycobacterial infection. skin biopsy taken for mycobacterial microscopy and culture isolated a heavy growth of mycobacterium abscessus. | 2001 | 11488830 |
| mastitis due to mycobacterium abscessus after body piercing. | we describe a patient with granulomatous mastitis due to mycobacterium abscessus that presented as a mass lesion and was associated with a pierced nipple. to our knowledge, this is the first reported case of mastitis due to m. abscessus and the first association of this organism with body piercing. | 2001 | 11389508 |
| pathologic quiz case. chronic pneumonia after gastrectomy. pathologic diagnosis: chronic aspiration lipoid pneumonia with mycobacterium abscessus. | 2001 | 11419993 | |
| novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16s-23s rrna gene spacer and restriction endonucleases. | a novel genus-specific pcr for mycobacteria with simple identification to the species level by restriction fragment length polymorphism (rflp) was established using the 16s-23s ribosomal rna gene (rdna) spacer as a target. panspecificity of primers was demonstrated on the genus level by testing 811 bacterial strains (122 species in 37 genera from 286 reference strains and 525 clinical isolates). all mycobacterial isolates (678 strains among 48 defined species and 5 indeterminate taxons) were amp ... | 2000 | 10699003 |
| diagnosis and management of atypical mycobacterium spp. infections in established laboratory zebrafish (brachydanio rerio) facilities. | two established zebrafish colonies experienced increased mortality and decreased reproductive performance. initial examination of several fish from one facility revealed hyperemic gills, petechia around the opercula, abdominal distention, and emaciation. affected fish had congested liver with inflammation and multifocal hepatic necrosis. large numbers of acid-fast-positive, rod-shaped bacteria were evident in multiple tissues and the blood. mycobacterium fortuitum was subsequently isolated from ... | 2000 | 11200575 |
| eradication of mycobacterium abscessus in a chronically infected patient with cystic fibrosis. | 2000 | 10973047 | |
| dna polymorphism of mycobacterium abscessus analyzed by infrequent-restriction-site polymerase chain reaction. | mycobacterium abscessus is an important pathogen that has been increasingly associated with many clinical and nosocomial infections. a reliable molecular typing scheme is essential for the epidemiological study of this rapidly growing mycobacterium. pulsed-field gel electrophoresis (pfge), considered to be the gold standard among molecular typing methods, has failed to provide satisfactory results in the molecular typing of this bacterium. a newly developed molecular typing method, infrequent-re ... | 2000 | 11039248 |
| skin and wound infection by rapidly growing mycobacteria: an unexpected complication of liposuction and liposculpture. the venezuelan collaborative infectious and tropical diseases study group. | to describe 10 patients with skin and soft tissue infection caused by rapidly growing mycobacteria after cosmetic liposuction and liposculpture. | 2000 | 11074697 |
| mycobacterium abscessus infection after facelift surgery: a case report. | 2000 | 10800917 | |
| multisite reproducibility of etest for susceptibility testing of mycobacterium abscessus, mycobacterium chelonae, and mycobacterium fortuitum. | a multicenter study was conducted to assess the inter- and intralaboratory reproducibility of the etest for susceptibility testing of the rapidly growing mycobacteria. the accuracy also was evaluated by comparing etest results to those obtained by broth microdilution. ten isolates (four of the mycobacterium fortuitum group, three of mycobacterium abscessus, and three of mycobacterium chelonae) were tested against amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem, and trim ... | 2000 | 10655363 |
| sporotrichoid dermatosis caused by mycobacterium abscessus from a public bath. | infections caused by nontuberculous mycobacteria (ntm) are usually associated with immunocompromised states. more recently, however, ntm infections are being diagnosed with greater frequency in patients lacking traditional risk factors. however, cutaneous infection with rapidly growing mycobacteria is uncommon, and diagnosis may be difficult. herein we present a case of sporotrichoid dermatosis on both forearms caused by mycobacterium abscessus in a 34-year-old female (case 1). mycobacterium abs ... | 2000 | 10824491 |
| mycobacterium abscessus infection in cystic fibrosis. colonization or infection? | we present a case of a patient with cystic fibrosis who was thought to be colonized with mycobacterium abscessus for 13 yr prior to developing clinically apparent mycobacterial infection. however, histologic evidence indicated that invasive mycobacterial disease was present from the onset. while accepting that chronic endobronchial colonization with atypical mycobacteria may occur in patients with cystic fibrosis, the repeated isolation of mycobacteria from the sputum of these patients should al ... | 2000 | 10673211 |
| a comparative study of the effects of uvc irradiation on select procaryotic and eucaryotic wound pathogens. | managing wounds infected with a mixture of several types of microorganisms such as bacteria (procaryotes) and fungi (eucaryotes) is a challenging clinical situation. the purpose of this study was to determine the effectiveness of ultraviolet light (uvc) in eradicating select procaryotic and eucaryotic organisms, in both pure culture and mixed cultures in vitro. five replications of each organism or mixture of organisms (10(6) organisms/ml singly or 10(15) organisms/ml mixed culture) were plated. ... | 2000 | 11889731 |
| in vitro susceptibilities of rapidly growing mycobacteria to telithromycin (hmr 3647) and seven other antimicrobials. | the antimicrobial activities of telithromycin (hmr 3647) and seven other antimicrobials against 94 strains of rapidly growing mycobacteria were determined. telithromycin at a concentration of 1 microg/ml inhibited mycobacterium peregrinum (100%), mycobacterium chelonae (80%), mycobacterium abscessus-mycobacterium mucogenicum (44.4%), and mycobacterium fortuitum (2.1%). all or most strains of m. peregrinum, m. fortuitum, and m. mucogenicum were inhibited by 2 microg of quinolones per ml. | 2000 | 10602744 |
| partial interferon-gamma receptor signaling chain deficiency in a patient with bacille calmette-guérin and mycobacterium abscessus infection. | complete deficiency of either of the two human interferon (ifn)-gamma receptor components, the ligand-binding ifn-gammar1 chain and the signaling ifn-gammar2 chain, is invariably associated with early-onset infection caused by bacille calmette-guérin vaccines and/or environmental nontuberculous mycobacteria, poor granuloma formation, and a fatal outcome in childhood. partial ifn-gammar1 deficiency is associated with a milder histopathologic and clinical phenotype. cells from a 20-year-old health ... | 2000 | 10608793 |
| cutaneous infections due to nontuberculous mycobacteria: histopathological review of 28 cases. comparative study between lesions observed in immunosuppressed patients and normal hosts. | to evaluate the histopathological features observed in patients with cutaneous infections due to nontuberculous mycobacteria (ntm) and to compare the histopathological patterns observed in immunosuppressed patients and normal hosts. twenty-eight biopsy specimens corresponding to 27 patients with cutaneous infections due to ntm were reviewed. eighteen biopsies corresponded to normal hosts (14 mycobacterium marinum, 2 mycobacterium chelonae, 1 mycobacterium terrae and 1 mycobacterium gordonae) and ... | 2000 | 10728814 |
| molecular characterization of mycobacterium abscessus strains isolated from a hospital outbreak. | 2000 | 10998394 | |
| susceptibility testing for mycobacteria. | mycobacterial susceptibility testing is important for the management of patients with tuberculosis and those with disease caused by certain nontuberculous mycobacteria. to help standardize methods used in the clinical microbiology laboratory for testing susceptibility of mycobacteria, the national committee for clinical laboratory standards (nccls) recently updated nccls document m24-t (published in 1995), which is the tentative standard for antimycobacterial susceptibility testing of mycobacter ... | 2000 | 11073754 |
| preliminary characterization of a mycobacterium abscessus mutant in human and murine models of infection. | the ability to persist in the host after the establishment of infection is an important virulence determinant for mycobacteria. mycobacterium abscessus is a rapidly growing mycobacterial species which causes a variety of clinical syndromes in humans. we have obtained a rough, wild-type human clinical isolate of m. abscessus (m. abscessus-r) and a smooth, attenuated mutant (m. abscessus-s) which spontaneously dissociated from the clinical isolate. we have found that m. abscessus-r is able to pers ... | 1999 | 10456919 |
| non-tuberculous mycobacterial tenosynovitis: a review. | the clinical characteristics, outcome and treatment of non-tuberculous mycobacterial tenosynovitis are reviewed. from lesions localized in the hand, 10 different species of non-tuberculous mycobacteria have been reported. the most common are mycobacterium marinum and mycobacterium kansasii. other less frequent organisms are mycobacterium avium complex, mycobacterium szulgai, mycobacterium terrae, mycobacterium fortuitum, mycobacterium chelonae, mycobacterium abscessus, mycobacterium malmoense an ... | 1999 | 10482048 |
| a new rapidly growing mycobacterial species, mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre. | scotochromogenic mycobacterial isolates from water-damaged parts of indoor building materials of a children's day care centre represented a phenetically and genetically distinct group of strains. a 16s rdna dendrogram (1243 bp) showed that the closest species to the new strain ma112/96t was mycobacterium abscessus. phylogenetic and phenetic analyses (100 characteristics) grouped the new isolates with m. abscessus, mycobacterium vaccae, mycobacterium aurum and mycobacterium austroafricanum. ribot ... | 1999 | 10028244 |
| abscesses due to mycobacterium abscessus linked to injection of unapproved alternative medication. | an unlicensed injectable medicine sold as adrenal cortex extract (ace*) and distributed in the alternative medicine community led to the largest outbreak of mycobacterium abscessus infections reported in the united states. records from the implicated distributor from january 1, 1995, to august 18, 1996, were used to identify purchasers; purchasers and public health alerts were used to identify patients. purchasers and patients were interviewed, and available medical records were reviewed. vials ... | 1999 | 10511524 |
| treatment of refractory disseminated mycobacterium abscessus infection with interferon gamma therapy. | 1999 | 10052395 | |
| hsp65 sequencing for identification of rapidly growing mycobacteria. | partial sequencing of the hsp65 gene was used for the identification of rapidly growing mycobacteria (rgm). a 441-bp fragment (a. telenti, f. marchesi, m. balz, f. bally, e. böttger, and t. bodmer, j. clin. microbiol. 31:175-178, 1993) was amplified and sequenced by an automated fluorescence-based method involving capillary electrophoresis. type strains of 10 rgm species were first studied. each species had a unique nucleotide sequence, distinguishing it clearly from the other species. a panel o ... | 1999 | 9986875 |
| multisite reproducibility of results obtained by the broth microdilution method for susceptibility testing of mycobacterium abscessus, mycobacterium chelonae, and mycobacterium fortuitum. | a multicenter study was conducted to assess the interlaboratory reproducibility of broth microdilution testing of the more common rapidly growing pathogenic mycobacteria. ten isolates (four mycobacterium fortuitum group, three mycobacterium abscessus, and three mycobacterium chelonae isolates) were tested against amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem, sulfamethoxazole, and tobramycin (m. chelonae only) in four laboratories. at each site, isolates were tested t ... | 1999 | 10325306 |
| infectious crystalline keratopathy and endophthalmitis secondary to mycobacterium abscessus in a monocular patient with stevens-johnson syndrome. | to describe the clinical and laboratory features of infectious crystalline keratopathy and endophthalmitis secondary to mycobacterium abscessus in a patient with stevens-johnson syndrome. | 1999 | 10030567 |
| improved methods for generation, sampling, and recovery of biological aerosols in filter challenge tests. | in preparation for filter efficiency tests and sampler comparison studies, methods of biological aerosol generation, sampling, and filter recovery were modified from previous studies. methods described include (1) techniques for generating aerosols that reduced nuisance particles to negligible levels and increased the cell culturability of mycobacterium abscessus by 30%, (2) sampling techniques that lowered the detectable range of biological particle size from 0.65 to 0.45 micron and reduced the ... | 1998 | 9586198 |
| variables affecting results of sodium chloride tolerance test for identification of rapidly growing mycobacteria. | the sodium chloride tolerance test is often used in the identification of rapidly growing mycobacteria, particularly for distinguishing between mycobacterium abscessus and mycobacterium chelonae. this test, however, is frequently unreliable for the identification of some species. in this study we examined the following variables: medium manufacturer, inoculum concentration, and atmosphere and temperature of incubation. results show that reliability is improved if the test and control slants are ... | 1998 | 9620376 |
| long-term laboratory contamination by mycobacterium abscessus resulting in two pseudo-outbreaks: recognition with use of random amplified polymorphic dna (rapd) polymerase chain reaction. | beginning in 1993, an increase in clinical isolates of mycobacterium abscessus was observed in a single hospital microbiology laboratory. this involved a cluster of four patients in june 1993 and five patients and a quality-control culture of distilled water in may 1994. twenty-three m. abscessus isolates recovered between 1991 and 1996 were compared by random amplified polymorphic dna polymerase chain reaction (rapd-pcr). sixteen of 21 clinical isolates recovered over a 6-year period and the di ... | 1998 | 9675472 |
| infection of epicardial pacemaker wires due to mycobacterium abscessus. | 1998 | 9502494 | |
| an outbreak of post-surgical wound infections due to mycobacterium abscessus. | an outbreak of post-operative wound infections due to mycobacterium abscessus is described. during a 5-month period 45 post-surgical patients developed wound infection, manifested by wound breakdown, cellulitis, and discharge and progressing slowly to suppuration and sinus formation. the majority (43/45) had undergone out-patient operations, and 40 had had surgery in the inguinal region. the source of infection was identified as contaminated tap water. a study revealed serious deficiencies in th ... | 1998 | 9639628 |
| mycobacterium abscessus peritonitis: a case report. | 1998 | 10182108 | |
| a single 16s ribosomal rna substitution is responsible for resistance to amikacin and other 2-deoxystreptamine aminoglycosides in mycobacterium abscessus and mycobacterium chelonae. | twenty-six clinical isolates of mycobacterium abscessus resistant to amikacin were identified. most isolates were from patients with posttympanostomy tube placement otitis media or patients with cystic fibrosis who had received aminoglycoside therapy. isolates were highly resistant (mics > 1024 microg/ml) to amikacin, kanamycin, gentamicin, tobramycin, and neomycin (all 2-deoxystreptamine aminoglycosides) but not to streptomycin. sequencing of their 16s ribosomal (r) rna revealed that 16 (94%) o ... | 1998 | 9607835 |
| photo quiz. subcutaneous abscess due to mycobacterium abscessus. | 1998 | 9502442 | |
| collection of three bacterial aerosols by respirator and surgical mask filters under varying conditions of flow and relative humidity. | a variety of respirator filters and surgical masks were challenged with three aerosolized bacteria: mycobacterium abscessus (m.a.) (a rod), staphylococcus epidermidis (s.e.) (a sphere), and bacillus subtilis (b.s.) (a rod). tests were conducted at two flow rates (45 and 85 l./min) and two humidity levels (30 and 70%). aerosols were measured with a total-particle, direct-reading, spectrometer and a viable particle cascade impactor. measurements up- and downstream of the filter or mask were used i ... | 1997 | 9375526 |