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density-dependent perception of triatomine bug bites.pots containing different densities of rhodnius prolixus were strapped on to the upper and lower arms and legs of six human volunteers. analysis of the volunteers' perception of the bugs' probing activity showed a clear linear trend of perception according to bug density. comparison with other results suggests that in humans as well as other vertebrate hosts, density dependent irritation from the bug bites and consequent disturbance and interruption of bugs' feeding could provide the mechanism b ...19863541809
trypanosoma cruzi and trypanosoma rangeli in saimiri sciureus from bolivia and saguinus mistax from brazil.examination of blood films for trypanosomes in primates housed at the tulane university delta regional primate research center showed that 47% (32/68) of the bolivian saimiri sciureus and 51% (68/135) of brazilian saguinus mistax harbored one or more types: trypanosoma (schizotrypanum) cruzi in 6% to 7% and trypanosoma (herpetosoma) spp. or trypanosoma (megatrypanum) spp. in 39% and 45%, respectively. trypanosomes were isolated from a sample of the infected monkeys and morphobiological studies w ...19863082228
establishment of baseline data on the insecticide susceptibilities of the chagas' disease vector rhodnius prolixus in venezuela. 19863103804
the affinity of the lectins ricinus communis and glycine maxima to carbohydrates on the cell surface of various forms of trypanosoma cruzi and trypanosoma rangeli, and the application of these lectins for the identification of t. cruzi in the feces of rhodnius prolixus.flagellates of trypanosoma cruzi (stock molino 1), obtained from the intestine of experimentally infected rhodnius prolixus, grown in cellular or acellular culture, as well as from the blood of infected mice, were examined by a direct fluorescence test using the lectins rca (ricinus communis-120) and sba (soy bean agglutinin; glycine maxima), conjugated with fluorescein isothiocyanate, for the detection of beta-d-galactose and alpha,beta-n-acetyl-d-galactosamine on the membranes of the flagellat ...19862877548
the surface morphology of the midgut cells of rhodnius prolixus stål (hemiptera: reduviidae) during blood digestion.the surface morphology of the midgut cells of rhodnius prolixus is examined using scanning electron microscopy. before feeding, the microvilli are devoid of any extracellular structures and can be observed in both fracture faces and surface views. by 3 days after feeding, patches of extracellular membrane layers are observed on the surface of the midgut cells and by 7 days the extracellular membrane layers form an incomplete sheet overlying the microvilli, such that the microvilli are no longer ...19862882663
[antidiuretic mechanism in rhodnius prolixus (stal, 1859) (hemiptera, reduviidae)].an antidiuretic mechanism is proposed for rhodnius prolixus, whose activity is manifested when the diuretic phase has terminated and is maintained until the insect has fed again. presumably this mechanism acts at the level of the proximal rectal sphincter and is inhibited by ingestion, mechanical distension of the gut and central disinhibition by decapitation. it is suggested that the antidiuretic activity is maintained by the nervous system and is modified when there is distention of the gut or ...19863302595
[laboratory maintenance of trypanosoma (herpetosoma) rangeli tejera, 1920].two laboratory maintenance systems of trypanosoma rangeli were compared. the maintenance by weekly subinoculations in tobie's culture medium and the intrafemoral inoculation of rhodnius prolixus with cultured flagellates, resulted in loss of infectivity of the metacyclic salivarian trypomastigotes for mice, ten months after maintenance in culture. with the system of cyclical passes through culture-rhodnius-mouse-culture-rhodnius, the infectivity of the metacyclic trypomastigotes for mice, was ma ...19863313550
induction of rhythmic modulation of haemolymph ecdysteroids in the insect rhodnius prolixus by treatments which elicit rhythmic ecdysis.the titer of moulting hormones (ecdysteroids) in fifth instar male rhodnius displays a gradual decline throughout the week preceding ecdysis when animals are maintained in constant light (ll). multiple sampling of haemolymph from the same animal reveals a constant rate of decline with no increases in titer observed. however, 20-hydroxyecdysone (20he) provokes increases in the titer of ecdysteroids which occur between 18 and 27 hr after injection. multiple injections of 20he provoke regular chang ...19863557060
salivary apyrase of rhodnius prolixus. kinetics and purification.the salivary apyrase activity of the blood-sucking bug rhodnius prolixus was found to reside in a true apyrase (atp diphosphohydrolase, ec 3.6.1.5) enzyme. the crude saliva was devoid of 5'-nucleotidase, inorganic pyrophosphatase, phosphatase and adenylate kinase activities. atp hydrolysis proceeded directly to amp and pi without significant accumulation of adp. km values for atp and adp hydrolysis were 229 and 291 microm respectively. ki values for atp and adp inhibition of adp and atp hydrolys ...19863010945
nurse cell-oocyte interaction: a new f-actin mesh associated with the microtubule-rich core of an insect ovariole.fluorescent staining of rhodnius prolixus ovarioles with rhodaminylphalloidin revealed an elaborate interconnecting meshwork of f-actin encasing the microtubule rich core. the thick actin struts have branches extending partially into the nurse cell lobes extending from the syncytical core. basally the network ends at the nurse cell-pre-follicular interface and does not extend into the trophic cords. light microscopy, tem and sem show the dense fibrous struts have numerous branch points creating ...198618620180
experimental analysis of rhodnius prolixus (insecta, hemiptera) embryogenesis. 19863749156
vitellin and vitellogenin characterization of triatoma infestans and related species.the vitellogenin-vitellin (vg-vn) of triatoma infestans is a glycolipoprotein with a mol. wt. of 220,000; is a high density lipoprotein (1.18-1.21 g/ml) with low electrophoretic mobility at ph 8.2. there are many common proteins present in females, males, nymphs and eggs, but none is a glycolipoprotein. the major band of protein revealed a mol. wt. of 43,000, it is an anodic protein which appears in all the fractions of saline gradient. males and females have a glycolipoprotein with electrophore ...19863111174
circadian control of a daily rhythm in hemolymph ecdysteroid titer in the insect rhodnius prolixus (hemiptera).the hemolymph levels of the insect molting hormone (ecdysteroid) during the week preceding ecdysis in fifth-instar male rhodnius prolixus have been determined using a radioimmunoassay. when animals are kept on light-dark cycles, the titer displays massive daily increases and decreases producing a daily rhythm. this rhythm is maintained with a period of approximately 24 hr in continuous darkness. the free-running period of the rhythm was determined at 24 and 28 degrees and found to be temperature ...19854043725
variations during the gonotrophic cycle in the titer of the myotropic ovulation hormone and the response of the ovarian muscles in rhodnius prolixus.the response of the ovarian muscles to a standard preparation of the myotropic ovulation factor from identified neurosecretory cells in the brain of rhodnius prolixus increases after feeding in both virgin and mated females. this increase is unaffected by removal of the neurosecretory cells and by increases imposed on the resting tension of the ovaries used in the bioassay. the hemolymph titer of the myotropic activity as determined by bioassay exhibits a peak after feeding in both mated and vir ...19853891505
glial cell contacts in insects: effects of feeding on intercellular junctions.the intercellular junctions associated with the modified glial cells of the perineurium have been examined in the ganglia and main abdominal nerves of the blood-sucking bug rhodnius prolixus, both before and and after feeding, by means of freeze-fracture and tracer studies. it was found that the pleated septate junctions found in the main abdominal nerve have many fewer septa than those found in the ganglion. these junctions appear to provide the flexibility needed for the movement of cells whic ...198518620150
laboratory repellent tests against rhodnius prolixus (heteroptera: reduviidae). 19853884812
[differentiation between trypanosoma cruzi and t. rangeli in the intestine of the vector rhodnius prolixus, based on the behavior of these flagellates with regard to the lytic activity of complement]. 19853892618
isozyme profiles of trypanosoma cruzi stocks from colombia and ecuador.a total of 74 of 82 domestic rhodnius prolixus from the same locality in eastern colombia were found to be infected with trypanosoma cruzi or t. rangeli. one of three domestic triatoma dimidiata from ecuador also showed t. cruzi infection. a total of 59 t. cruzi stocks from these and five other localities in colombia were isolated from man, marsupials and triatomine bugs. cellulose-acetate electrophoresis of nine or ten enzymes characterized all t. cruzi stocks as zymodeme 1 (reference clone sil ...19853896169
parasitism of telenomus costalimai (hymenoptera: scelionidae) on rhodnius prolixus (hemiptera: reduviidae) under laboratory conditions: effects of density. 19853908680
feeding, molting, and egg production in rhodnius prolixus (hemiptera: reduviidae) fed repeatedly on the same swiss mouse hosts. 19853908681
a protein diet initiates oogenesis in rhodnius prolixus.oogenesis in r. prolixus females is induced by feeding blood, blood plasma, a suspension of washed erythrocytes, or 5% solutions of egg albumin, bovine serum albumin or hemoglobin. unfed females and those fed saline, casein hydrolysate, dextran or sucrose do not initiate oogenesis. the juvenile hormone analogue epoxygeranylgeraniol methyl ester induced oogenesis in unfed females as well as in ethoxyprecocene ii-treated insects. the results show that a protein meal was sufficient to initiate ooge ...19853913479
[natural hosts of trypanosoma cruzi in french guiana. high endemicity of zymodeme 1 in wild marsupials].during an epidemiological survey in french guiana, three species of marsupiala were found infected by trypanosoma cruzi with high infection rates (30,8% for didelphis marsupialis). six triatomine bug species were recorded, five of them being well known vectors of chagas' disease. iso-enzyme characterization of 22 stocks isolated (16 from d. marsupialis, 3 from philander opossum and 3 from rhodnius prolixus) revealed that they were all related to zymodeme 1 of miles. the silvatic cycle is endemic ...19853923891
trypanosoma (herpetosoma) rangeli tejera, 1920: mouse model for high, sustained parasitemia.the neotropical mammalian parasite trypanosoma (herpetosoma) rangeli tejera, 1920 is difficult to study due to the scarcity of blood forms in the vertebrate host. high and persistent parasitemias (up to 7 times the original inoculum at the peak, and persisting for up to 2 wk) were obtained by i.p. inoculation of infant (6.0 g) male white mice (nmri strain) with 15 x 10(3) trypomastigotes/g body weight from 12-day-old cultures of the "dog-82" strain of t. rangeli. this strain was cultured 15 mo a ...19854032147
[prolixase, a fibrinolytic enzyme isolated from rhodnius prolixus (heteroptera, reduviidae): antigenic properties].antigenic properties of prolixase are studied showing that this fibrinolytic enzyme, induces in the rabbit, the formation of precipitating but slightly, hemagglutinating homologous antibodies.19852418798
morphogenetic effects of precocene ii on the immature stages of rhodnius prolixus. 1985366817
removal of insect basal laminae using elastase.we have used the enzyme elastase to remove the basal lamina of epithelia from two insects: the upper malpighian tubules of rhodnius prolixus and imaginal discs of drosophila melanogaster. removal of the basal lamina was confirmed using scanning and transmission electron microscopy. use of the technique on the malphighian tubules of rhodnius reveals for the first time the three-dimensional organization of the circumferential folds of the basal plasma membrane. elastase is much more effective in r ...19846431633
passage of solutes through walls of malpighian tubules of rhodnius by paracellular and transcellular routes.the walls of isolated upper malpighian tubules of the insect, rhodnius prolixus, are much more permeable to small relatively unchanged solutes (ethanol, xylose, and mannitol) than to larger or more charged solutes (acetate, glycine, tyrosine, and inulin). the more permeable solutes rapidly reach concentrations in the tubule cells equivalent to their concentrations in the bathing medium; the less permeable solutes do not penetrate into the cells. the time course of accumulation of permeable solut ...19846426328
epidemiology of trypanosoma cruzi in the oriental plains of colombia.epidemiologic studies to define the domiciliary and extradomiciliary transmission cycles of trypanosoma cruzi and trypanosoma rangeli in the oriental plains of colombia were conducted in the gallery forests near carimagua and el porvenir. one-hundred and seven palm trees belonging to nine genera were examined; triatomines were found in only three palm species, the leaves of which are locally used for roof thatching: 2/29 maximiliana elegans, 1/7 mauritia flexuosa and 7/7 scheelea sp. bugs were a ...19846439062
[finding of rhodnius prolixus stal, 1859, in babassu palm trees]. 19846443714
dissociation of insect malpighian tubules into single, viable cells.the malpighian tubules of insects are generally composed of more than one cell type. in the hemipteran rhodnius prolixus, the tubules are divided into two regions, termed the upper and lower tubules, each of which is composed of a distinct cell type. in the dipteran aedes taeniorhynchus, primary and stellate cells are interspersed throughout the length of the tubules. we report here techniques for the dissociation of the malpighian tubules of both of these species into single cells. tubules are ...19846533146
secretion by the malpighian tubules of rhodnius prolixus stal: electrical events.transepithelial and intracellular potentials have been simultaneously recorded from rhodnius upper malpighian tubules before and after stimulation of fluid secretion. the transepithelial electrical response to the diuretic hormone mimic 5-hydroxytryptamine (5-ht) was triphasic; recordings of intracellular potential changes indicated that the three phases represented successive events at the apical membrane. depolarizations produced by increasing the bathing medium potassium concentration indicat ...19846379094
effects of juvenile hormone analogue on ecdysis prevention induced by precocene in rhodnius prolixus (hemiptera:reduviidae).precocene ii, added to the meal of fourth-instar larvae of rhodnius prolixus (25 micrograms/ml of blood), induced an increase in the duration of the molting cycle. this effect was related to the decrease of both the nuclear area of the prothoracic gland cells and the mitotic activity in epidermal cells. juvenile hormone analogue applied topically (60 micrograms/insect) together with precocene ii treatment avoided atrophy of the prothoracic glands and induced a higher number of epidermal mitosis ...19846398393
studies on trypanosoma rangeli tejera, 1920. vii--its effect on the survival of infected triatomine bugs.the pathological effects of trypanosoma rangeli on rhodnius prolixus and r. robustus, and the relation of mortality to infection, were studied under laboratory conditions. frequent observations revealed that when the first instar nymphs of r. prolixus and r. robustus were infected with t. rangeli, survival of the bugs during the stages of development to the adult stage decreased. this decrease was statistically significant when compared with uninfected control-bugs, indicating that t. rangeli is ...19846399091
serotonin modulation of the release of sequestered [3h]serotonin from nerve terminals in an insect neurohemal organ in vitro.exogenous tritiated serotonin ([3h]5-ht) is taken up by and released from serotonin-containing fibers within abdominal nerves of rhodnius prolixus during in vitro incubations. sequestered [3h]5-ht behaves as expected of an endogenous neurosecretory product in this system. release is ca2+-dependent during both k+-induced and physiologically induced secretory episodes. the kinetics of the release of sequestered label parallels the kinetics of release of endogenous neurohormones. preloaded preparat ...19846380644
cytological evidence for serotonin-containing fibers in an abdominal neurohemal organ in a hemipteran.the abdominal neurohemal organs of the hemipteran rhodnius prolixus contain an extensive serotonin containing arborization. endogenous serotonin within fibers and terminals in the neurohemal area were detected with histochemical and immunocytological techniques. the abdominal nerves which contain the neurohemal organs selectively sequester exogenous serotonin. serotonin and its metabolites are biochemically detected within the mesothoracic ganglion, which is a known source of projections into th ...19846380643
cytophysiological correlations between prothoracic gland activity and hemolymph ecdysteroid concentrations in rhodnius prolixus during the fifth larval instar: further studies in normal and decapitated larvae.hemolymph ecdysteroid titers in fifth instar larvae of rhodnius prolixus were determined by radioimmunoassay, and their prothoracic glands were excised and examined by electron microscopy. during the last larval instar, the titer of ecdysteroid increased between the head-critical period until day 13, at which time the peak titer was 3100 pg 20-hydroxyecdysone equivalents/microliter. the activation of secretory cells at the time of the second period of prothoracicotropic hormone release was corre ...19846370785
diphosphoglycerate rather than atp as a physiological phagostimulant factor for rhodnius prolixus. 19836339262
factors controlling the volume of feces produced by triatomine vectors of chagas' disease.triatomine species influenced volume of feces produced; triatoma dimidiata produced the largest volume of feces followed by panstrongylus megistus, rhodnius prolixus, and triatoma infestans. moreover, stage and sex affected fecal production; 5th-stage female nymphs excreted the largest volume of feces. the amount of blood ingested was significantly correlated with the volume of feces produced in 10 out of 11 experimental groups. triatome size and volume of feces produced were less closely correl ...19836142633
studies on trypanosoma rangeli tejera, 1920. vi. developmental pattern in the haemolymph of rhodnius prolixus.the morphological sequence of trypanosoma rangeli development in the body cavity of rhodnius prolixus is described. the metacyclic trypanosome is the product of successive division and transformation during the intra and extracellular development in the haemocoele. the significance of the early invasion of t. rangeli into the haemolymph is discussed. the epidemiological importance of the developmental pattern of t. rangeli in the vectors haemolymph and the host-response to the parasite are consi ...19836400168
susceptibility of insect-borne, metacyclic forms of trypanosoma cruzi to antibody-mediated mechanisms of destruction.the effect of passive antibody transfer against infection with vector-borne, metacylic forms of trypanosoma cruzi, and possible mechanisms of immunologic lysis of these organisms, were examined in this work. anti-t. cruzi antibodies from mice surviving an infection conferred marked protection against challenge with a lethal dose (2,000 organisms) of metacyclic flagellates isolated from the reduviid insect rhodnius prolixus. in vitro lysis of these parasites by immune sera from mice or chronic ch ...19836418013
diuresis from stimulation of left atrial receptors in dogs: further purification of the causative agent from plasma.stimulation of atrial receptors by distension of a balloon in the left atrium of anaesthetized dogs results in a reflex diuresis mediated by an unknown humoral agent. this paper reports the further purification from plasma of acetone extracts of the humoral agent which may mediate the diuretic response. activity of the humoral agent was detected using the malpighian tubules of rhodnius prolixus. gel filtration of the acetone extracts on sephadex g-25 and g-100, followed by dialysis of the fracti ...19836856747
abolition of atrial receptor diuresis and of release of humoral agent by cooling the vagal nerves.stimulation of atrial receptors in anaesthetized dogs results in a reflex increase in urine flow mediated by vagal myelinated fibres and, in part, by a humoral agent of unknown identity. the humoral agent has been detected by an in vitro preparation of the malpighian tubules of rhodnius prolixus. the purpose of this investigation was to determine whether the present extract of plasma purporting to contain the humoral agent is also related to the reflex diuresis. in nine chloralose anaesthetized ...19836856748
studies on trypanosomes in the taiwan monkey.fifty-three or 9% of 594 taiwan monkeys, macaca cyclopis, were found infected with a trypanosome species. the parasites were recovered from monkey blood in diaphasic blood-agar culture medium with a locke's solution overlay. trypanosomes were found on only a few thick blood smears and no dividing or multiplying forms were found. some of the organisms had a free-flagellum (4.7 microns), an undulating membrane and both ends of the body were pointed. the total length averaged 41.7 microns; nucleus ...19836673131
cross-reactivity of vector-borne metacyclic forms of trypanosoma cruzi with mammalian and culture stages.metacyclic forms of trypanosoma cruzi isolated from the hindgut of infected insect vectors (rhodnius prolixus) were found to be immunologically cross-reactive with cultured epimastigote, amastigote, and metacyclic stages of the parasite as well as with bloodstream trypomastigote forms by direct agglutination and indirect immunofluorescence techniques. sera specific for each of these forms of the parasite systematically yielded maximal antibody titers when measured against the homologous antigen, ...19836195335
functional design of microvilli in the malpighian tubules of the insect rhodnius prolixus.the malpighian tubules of rhodnius prolixus are divided into two regions; the upper tubule, which is the site of isosmotic secretion and haemolymph filtration, and the lower tubule where water and kcl are resorbed. in the upper tubule the microvilli are arranged in clumps consisting of several hundred microvilli lying closely parallel. the microvillar plasma membranes do not touch but are held approximately equal to 16 nm apart along the full length of the microvilli. as a consequence, the extra ...19836348047
crystallized hemoglobin in rhodnius prolixus after a blood meal on guinea-pig.several blood-sucking arthropods, after a blood meal, are able to store the hemoglobin from their hosts in a crystalline state in their digestive system. guinea-pig hemoglobin crystallizes in the stomach of the reduviid bug rhodnius prolixus in two different crystal types. we show them to be crystallographically identical and to contain the same liganded state of hemoglobin, i.e. they represent different habits of the same crystal modification. the hemoglobin crystallizes in oxy-form and ages in ...19836360705
morphology of the dorsal vessel in the abdomen of the blood-feeding insect rhodnius prolixus.the dorsal vessel (dv) in the abdomen of the blood-feeding insect rhodnius prolixus was divided functionally into two regions, the heart, into which haemolymph entered the dv through four pairs of ostia located in abdominal segment vii, and the aorta, along which the haemolymph was propelled from abdominal segment vi to the thorax. osmium-fixed whole mounts revealed the dv to consist of spirally arranged striated muscle fibers and to possess two rows of ventrally attached longitudinal fibers ext ...19832187100
description of new fibrinolytic enzyme, isolated from the haemolymph of rhodnius prolixus stal (heteroptera reduviidae).a new fibrinolytic enzyme, isolated from the haemolymph of rhodnius prolixus stal (heteroptera, reduviidae) has been discovered. this enzyme does not act by activating plasminogen to plasmin. however, the split products obtained with this new enzyme inhibit fibrin formation in normal plasma. this enzyme seems to act specifically on the proteins involved in blood coagulation. its origin has induced us to name it prolixase.19826762857
[feeding preferences in 3 species of triatominae (hemiptera: reduviidae) in experimental conditions].feeding preferences of three species of triatomines (triatoma infestans, t. dimidiata, and rhodnius prolixus) on four vertebrate hosts (opposum, dog, chicken and toad) were studied in a specially designed cage during daylight (2 hours) and at night (14 hours). t. infestans and r. prolixus remained close to the host after feeding, while t. dimidiata wandered a short distance away, apparently for protection against preying by the host. r. prolixus showed the lightest values of ingestion in relatio ...19826763728
[rhodnius prolixus stal, 1859, in goiás, brazil. preliminary report]. 19826764950
the effect of lampit on trypanosoma rangeli in experimentally infected mice.mice experimentally inoculated with metacyclic forms of trypanosoma rangeli were given orally 10, 25, 50 or 100 mg/kg of body weight/day of lampit on 20 consecutive days. the results of combined examinations of fresh blood, giemsa stained blood preparations, blood cultures, blood concentration, repeated xenodiagnosis with rhodnius prolixus and blind passages via triatomine bites to other mice demonstrated that a dose of 50 to 100 mg/kg/day of the drug eliminated t. rangeli from the blood of all ...19826814024
the activity of nifluridide on reduviids and rodent and human trypanosomes.the ability of nifluridide to kill reduviids was assayed in mice fed 7 ppm in diet and on cattle injected subcutaneously at 5 mg/kg body weight. nifluridide was systemically active against triatoma infestans on mice and rhodnius prolixus on cattle. no effects on trypanosoma (schizotrypanum) cruzi could be detected in the intestinal contents of triatoma infestans killed by the compound. in vitro and in vivo studies were conducted to determine the effects of nifluridide on trypanosomes growing in ...19826815915
developmental stages of trypanosoma cruzi-like flagellates in cavernicola pilosa.the developmental stages of trypanosoma cruzi ssp., found in the intestinal tract of cavernicola pilosa, are described and measurements given for nine life stages. the frequencies of the various stages in foregut, midgut and hindgut of the triatomines are provided; parasites were rare in the foregut and metatrypomastigotes were seen only in the mid- and hindguts. all adult bugs examined harboured intestinal infections of t. cruzi-like flagellates, large clumps of amastigotes were frequently obse ...19826820697
the effects of precocene ii on reproduction and development of triatomine bugs (reduviidae: triatominae).precocene ii is a botanically derived chemical that inhibits the production of juvenile hormone (jh) in insects. the effects of this anti-jh compound on molting and growth by rhodnius prolixus and triatoma dimidiata and reproduction in r. prolixus were tested and the efficacy of precocene ii as a fumigant was assessed. precocene ii induced precocious metamorphosis in both species when applied by either contact exposure or fumigation, and this effect could be prevented by juvenile hormone replace ...19827041667
ovarian motility in mated rhodnius prolixus requires an intact cerebral neurosecretory system. 19827129081
diphosphoglycerate and phytic acid as feeding stimulants for the blood-feeding bug rhodnius prolixus.1. the compounds 2,3-diphosphoglycerate (dpg), phytic acid (pa) and adenosine triphosphate (atp) were tested as gorging stimulants for rhodnius prolixus. all three are potent phagostimulants, with ed50 values less than 10 microm; in contrast, only atp stimulates the black fly simulium venustum, with an ed50 of 13 microm. 2. equimolar mixtures of dpg and atp showed pronounced synergism, causing 75% of test r. prolixus to gorge at a dose of 0.3 microm. 3. the response to dpg and pa supports the hy ...19826124351
effects of ecdysone on the metamorphosis and ecdysis prevention induced by precocene ii in rhodnius prolixus. 19817026357
platelet antiaggregating activity in the salivary secretion of the blood sucking bug rhodnius prolixus. 19817016577
development of nurse cell-oocyte interactions in the insect telotrophic ovary (rhodnius prolixus).the establishment of reorganization of intercellular bridges during larval-adult ovarian differentiation is the basis of the syncytial nature of the adult hemipteran telotrophic ovary. the formation, in the late differentiation phase, of groups of closely arranged nurse cell nuclei occupying a common cytoplasm results from membrane fusions. oocyte-oocyte intercellular bridge systems later are modified to form the trophic cords. the trophic core, which undergoes a restructuring during the late di ...19817031984
diuresis from stimulation of left atrial receptors: adh and the malpighian tubules of rhodnius prolixus.stimulation of left atrial receptors causes a diuresis partly through a blood borne agent. malpighian tubules of rhodnius prolixus can detect a blood borne agent during the diuresis. it has been suggested that the agent is adh but this is in dispute. in the present investigation, vasopressin (1-300 mu i.u. cm-3) was found to have no effect on malpighian tubule secretion when added to plasma, rhodnius ringer and rhodnius ringer + 5-ht. in addition, it was shown that incubation with sodium thiogly ...19816910734
an energy budget for rhodnius prolixus (hemiptera: reduviidae) under laboratory conditions. 19817021833
5-hydroxytryptamine-stimulated mitochondrial movement and microvillar growth in the lower malpighian tubule of the insect, rhodnius prolixus.rapid initiation of ion transport occurs in the lower malpighian tubule of the insect rhodnius prolixus following feeding in vivo or stimulation with 5-hydroxytryptamine (5-ht) in vitro. using the electron microscope, we have conducted a morphometric analysis of cells in the lowest one-third of the lower tubule, demonstrating that 5-ht also induces mitochondrial movement and microvillar growth simultaneously with, but independent of, the onset of ion transport. mitochondria move from a position ...19817031069
lectins of distinct specificity in rhodnius prolixus interact selectively with trypanosoma cruzi.lectins of different activities were found in the crop, midgut, and hemolymph of the insect rhodnius prolixus. these were not specific for n-acetyl-d-mannosamine, alpha-n-acetyl-d-galactosamine, and alpha- and beta-galactose, respectively. lectin receptors were detectable in epimastigote but not in trypomastigote forms of trypanosoma cruzi, a protozoan parasite of the insect and of humans.19817006082
experimental infections in venezuelan lizards by trypanosoma cruzi.virulent trypomastigotes of the y strain of trypanosoma cruzi were administered to tropidurus hispidus, ameiva ameiva, cnemidophorus lemniscatus, polychrus marmoratus, and phyllodactylus ventralis (sauria). intraperitoneal and subcutaneous inoculations of lizards with mouse blood or with feces of infected rhodnius prolixus (reduviidae, triatominae), as well as forced ingestion of triturated rhodnius, produced no parasitaemias detectable either directly or by xenodiagnosis, while control mice bec ...19816115559
nurse cell-oocyte interaction in the telotrophic ovarioles of an insect, rhodnius prolixus.microinjection of intracellular tracers fluorescein, procion yellow, lucifer yellow and horseradish peroxidase unequivocally showed the syncytial structure of the tropharium and its interaction with the oocytes. the tropharium tip is a separate isolated compartment. finger-like nurse cell projections comprising the syncytial tropharium interact via gap junctions along their abutting membranes and also via large cytoplasmic continuities at the central trophic core. the trophic cords connecting th ...19817013152
effects of sublethal dose exposure to dieldrin on rhodnius prolixus (hemiptera: reduviidae) under laboratory conditions.the effects of a sublethal dose of dieldrin (7.2 x 10(-5) g/day) were evaluated on oxygen consumption, survivorship, moulting and reproduction of rhodnius prolixus. no difference was found in oxygen consumption between treated and control male insect. survivorship proved to be very much affected by the sublethal exposure, but this was strongly dependent on the feeding stage of the individuals. moulting times were prolonged by 2 to 3 days depending on the instar, but the number of individuals tha ...19806104425
[ultrastructure of the antennal sensillae of the bug, rhodnius prolixus (hemiptera, reduviidae)].on the antenna of the bug r. prolixus we found numerous receptors which can be classified into three categories: mechanoreceptors (sensilla chaetica), olfactory (sensillae trichodea, basiconica and caeloconica) and gustatory (sensilla trichodea) receptors. the number, size and distribution on the antenna of each type have been recorded. a description of each of these sense organs is given. tactile hairs are present on all of the antennal segments while thin-walled chemoreceptors are limited to s ...19807007992
detection of trypanosoma rangeli by salivation of infected rhodnius prolixus on glass slides. 19807008724
trypanosoma cruzi development is independent of protein digestion in the gut of rhodnius prolixus. 19807012289
the penetration of the salivary glands of rhodnius prolixus by trypanosoma rangeli.ultrastructural studies of the mechanism of penetration of the salivary gland of the reduviid bug rhodnius prolixus by trypanosoma rangeli showed that trypanosomes from the haemocoele penetrate the outer "membranes" of the gland flagellum foremost, disrupting the inner layers, to pass between the muscle cells to reach the gland cell basement membrane. this latter is also penetrated flagellum foremost, the parasite invaginating the gland cell plasmalemma beneath, to create a vacuole in which the ...19806994382
[resistance to fast in venezuelan triatomidae (hemiptera, reduviidae). i- rhodnius prolixus stal]. 19807001606
early invasion of trypanosoma rangeli into the haemolymph of rhodnius prolixus. 19807001694
role of angiotensin ii and bradykinin in the diuresis caused by stimulation of atrial receptors.the diuretic response to stimulation of left atrial receptors, by distending balloons located either at the pulmonary vein-left atrial junctions or in the body of the left atrium, was studied during blockade of the generation of angiotensin ii using a continuous infusion of angiotensin converting enzyme inhibitor, sq 14,225, in anaesthetized dogs. despite blockade of angiotensin conversion, known also to potentiate the activity of bradykinin, a diuretic response was obtained in each dog; the res ...19807005924
effects of a mammalian cholesterol biosynthesis inhibitor on adipocyte ultrastructure and metamorphosis in rhodnius prolixus (hemiptera).a potent inhibitor of cholesterol biosynthesis in rodents [bmva; 5-(4-biphenylyl)-3-methylvaleric acid] inhibits metamorphosis of 4th stage larvae of the blood-sucking bug rhodnius prolixus.19806998718
electrical activity of neurosecretory axons from the brain of rhodnius prolixus: relation of changes in the pattern of activity to endocrine events during the moulting cycle.ongoing electrical activity was recorded from the axons of neurosecretory cells from the brain of 5th instar rhodnius prolixus throughout the moulting cycle. dramatic changes in both the frequency and pattern of electrical activity occur at specific times during the cycle, enabling the timing of release of neurohormones from the brain to be inferred. the level of transport of stainable secretion appears to be closely coupled to the electrical activity at all times. activity is low in unfed rhodn ...19807378760
the development of the female accessory gland in the insect rhodnius prolixus.the specialized cell types and two distinct regions of the adult rhodnius prolixus cement gland develop from a simple pseudostratified epithelial tube during the 20-22 days of the fifth stadium. feeding initiates the first phase, proliferation. cells round up and divide tangentially to the lumen. following the proliferation phase, differentiative mitoses occur and differentiation, resulting in secretory units (consisting of a ductule, gland cell and cuticular lining), ensues in the distal region ...19807010677
development and cellular differentiation of an insect telotrophic ovary (rhodnius prolixus).differentiation events accompanying the larval-adult ovarian transformation in rhodnius prolixus can be divided into three phases: proliferative phase (unfed to 3 days post-feed or dpf), early differentiation phase (9-15 dpf) and late differentiation phase (16 dpf to moult at 21 dpf). ovarioles remain morphologically larval until feeding initiates development. the unfed ovariole contains germ cells surrounding a central trophic core region with the 'germarial lumen' occupying the basal region of ...19807010676
the ultrastructure of the female accessory gland, the cement gland, in the insect rhodnius prolixus.the cement gland of rhodnius prolixus is an epidermally derived tubular gland consisting of a distal synthetic region and a proximal muscular duct region. the synthetic region consists of numerous secretory units joined to a central chitinous duct via cuticular ductules. proteinaceous secretion, synthesized by the goblet-shaped secretory cell, passess through the delicate cuticular lattice of a ductule-end apparatus and out through fine ductules to the central duct. secretory cells are rich in r ...19807001676
effect of diet viscosity on the operation of the pharyngeal pump in the blood-feeding bug rhodnius prolixus.1. the rate of pumping and total number of pump strokes was recorded whilst rhodnius prolixus fed on measured amounts of artificial diets. 2. increasing the viscosity of the diet caused a decline in both the frequency with which the pharyngeal pump operates and the average stroke volume of the pump. 3. in 5th-instar rhodnius, the stroke volume tends toward a maximum value of about 60 nl at viscosities lower than about 3 cp. 4. feeding rates (volume per unit time) agree with poiseuille's law at h ...197911799696
an unusual cell surface modification: a double plasma membrane.the occurrence of an unusual double plasma membrane structure is reported; it has been studied in conventional thin sections, after lanthanum-impregnation and with freeze-fracturing. this modification of the plasmalemma is found where the luminal cell membrane (i membrane) of gut microvilli in the haematophagous insect, rhodnius prolixus, is surrounded by a second, outer membrane (o membrane), the 2 separated from one another by a highly regular i-o space of about 10 nm. lanthanum impregnation r ...1979528589
changes in hormone activity of single neurosecretory cell bodies during a physiological secretion cycle.single neurosecretory cell bodies were dissected from the ventral ganglionic mass of rhodnius prolixus, lysed in distilled water, and bioassayed for diuretic hormone (dh) activity on isolated malpighian tubules. dh was found in a least 10 somata within the ganglion; electron micrographs of isolated cells show a large population of elementary neurosecretory granules. quantitative measures of hormone activity were made by bioassaying somata from unfed 5th instar larvae, and at the following times ...1979421131
ecdysone, juvenile hormone and oögenesis in rhodnius prolixus. 1979387882
domiciliary biting frequency and blood ingestion of the chagas's disease vector rhodnius prolixus ståhl (hemiptera: reduviidae), in venezuela.demolition of a rural house in the state of cojedes, venezuela, provided a collection of 7.934 rhodnius prolixus of which a random sample of 1,415 was weighed within 48 hours. the field weights, coupled with laboratory information of weight loss (in %) with time, average blood ingestion and meal size sufficient to promote moulting, were used to estimate biting rate under domiciliary conditions. the results show that in this particularly highly infested house, the r. prolixus population bites, on ...1979112731
a comparison of challenge with trypanosoma cruzi blood-stream trypomastigotes and metacyclic trypomastigotes from rhodnius prolixus in mice immunized with killed antigens.groups of cd-1 mice were immunized with vaccines prepared from freeze-thawed or ultra-sonicated epimastigotes, blood trypomastigotes, or "plasma antigen", of trypanosoma cruzi strains y, m1 and tulahuen. the mice were challenged by the injection of blood stream trypomastigotes obtained from mice, or of metacyclic trypomastigotes harvested from the rectum of rhodnius prolixus. both challenges induced virulent infections in control mice. blood-stream trypomastigotes killed mice more rapidly than t ...1979122124
insect axopods.using the elctron microscope we have found axopods, a cell organelle previously undescribed in multicellular animals, in the lower malpighian tubule of the insect. rhodnius prolixus. the axopods, which are 0.2 to 0.8 micrometer in diameter and 10 or more micrometer in length, derive from the luminal surface of the tubule and contain an array of 1 to about 46 microtubules each. these microtubules arise within the cell near the cell junctions or near clumps of mitochondria. uric acid crystals whic ...1979370127
diuresis from left atrial receptors: effect of plasma on the secretion of the malpighian tubules of rhodnius prolixus.1. stimulation of left atrial receptors by distension of a balloon in the lumen of the left atrium of anaesthetized dogs was shown to result in an increase in urine flow. plasma samples obtained from these dogs during control periods and during periods of stimulation were applied to the malpighian tubules of rhodnius prolixus. 2. it was found that the tubules suspended in test plasma secreted at a significantly lower rate than those suspended in control plasma. 3. these differences were also evi ...1979383951
larval competition between telenomus costalimai (hymenoptera: scelionidae) and ooencyrtus trinidadensis venatorius (hymenoptera: encyrtidae) after simultaneous oviposition in rhodnius prolixus eggs (hemiptera: reduviidae). 1979396374
evidence of microfilament-associated mitochondrial movement.the mitochondria in the lower malpighian tubule of the insect rhodnius prolixus can be stimulated by feeding in vivo and by 5-hydroxytryptamine in vitro, to move from a position below the cell cortex to one inside the apical microvilli. during and following their movement into the microvilli, the mitochondria are intimately associated with the microfilaments of the cell cortex and microvillar core bundle. bridges approximately 14 nm in length and 4 nm in diameter are observed connecting the micr ...1979397369
[action of insect development inhibitors on aedes aegypti l. mosquitoes and on rhodnius prolixus stal. but. ii. the juvenile activity of metoprene and structurally close compounds]. 1979353473
comparative xenodiagnosis with three triatomine species of different hosts with natural and experimental chronic infections with trypanosoma (schizotrypanum) cruzi.simultaneous xenodiagnosis was made of 29 patients with chronic chagas's disease, six naturally infected opossums (didelphis azarae) and of a rhesus monkey with an experimental chronic infection with trypanosoma (schizotrypanum) cruzi (peru strain). patients and opossums were from an endemic area in the state of bahia, brazil, where panstrongylus megistus is the sole domiciliary vector of the disease to man. various instars of p. megistus triatoma infestans and rhodnius prolixus were used. the p ...1978416525
[demonstration of an enzyme inhibiting coagulation of blood ingested by the blood-sucking insect, rhodnius prolixus (heteroptera, reduviidae)].a new fibrinolytic enzyme, "the prolixase", was isolated and purified from the haemolymph of rhodnius prolixus. the mode of action of the enzyme was studied and compared to that of extracts from salivary glands and midgut of the insect. it appears that the prolixase is involved in inhibiting the coagulation of the blood ingested by rhodnius during its blood meal. the mode of action of the enzyme in this phenomenon is discussed.1978747454
a microhymenopteran parasite of eggs of psammolestes arthuri (hemiptera: reduviidae) and observations of experimental parasitism of eggs of rhodnius prolixus (hemiptera: reduviidae). 1978344883
triatomines as experimental vectors of trypanosomes of asian monkeys.systematic surveys of the wild macaques of south asia by blood culture resulted in the discovery that trypanosomiasis is enzootic in the simians of indonesia, malaysia, india, and thailand. the isolates obtained differ in morphology, metabolism, and ability to multiply in arthropods. following this discovery, interest focused on possible transmissions of these trypanosomiases. laboratory-reared and wild-caught insects were studied to determine which are satisfactory intermediate hosts and potent ...1978417639
purification and characterization of a sulfhydryl-dependent protease from rhodnius prolixus midgut. 197828087
the action of juvenile hormone on follicle cells of rhodnius prolixus in vitro: the effect of colchicine and cytochalasin b. 1977892420
high-molecular-weight forms of diuretic hormone from rhodnius prolixus [proceedings]. 1977913782
some lipid classes in the fat-body of rhodnius prolixus. 1977614757
sequence of ultrastructural changes induced by activation in the posterior neurosecretory cells in the brain of rhodnius prolixus with special reference to the role of lysosomes.the posterior neurosecretory cell (pnc) group in the brain of rhodnius prolixus is composed of five ultrastructurally identical cells. the pnc were examined in the unfed fifth instar and at seven stages (from 15 min to 14 days) after activation was initiated by feeding. each stage examined revealed successive changes in morphology which can be related to the synthesis, maturation, storage and transport of neurosecretory material. it is suggested, in particular, that the lysosomal system (dense b ...1977337573
control of protease secretion in the intestine of fifth instar larvae of rhodnius prolixus. 1977323372
hematophagous insects as vectors for frog trypanosomes.experimental infections of three hematophagous arthropods (rhodnius prolixus, aedes aegypti, and culex pipiens) with a trypanosome of the trypanosoma rotatorium complex found in the frogs hyla crepitans and leptodactylus insularum revealed that a. aegypti is a good host for the flagellate; the course of development in the intestinal tract of the mosquito is described from 15 minutes to 168 hours. c. pipiens showed only low intestinal infections and r. prolixus did not permit development of the p ...1977615322
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