Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse. | antisera prepared against balb/c meth a sarcoma in syngeneic or compatible f1 mice recognize a protein with an apparent molecular weight of 53,000 in extracts of [35s]methionine-labeled transformed balb/c cells. this component, designated p53, was not detected in normal adult mouse fibroblasts, lymphoid cells, or hematopoietic cells or in mouse embryo cells or 3t3 cells. an extensive variety of antisera, including alloantisera and heterologous antisera directed against structural antigens of mur ... | 1979 | 221923 |
| a murine sarcoma virus-associated protein kinase: interaction with actin and microtubular protein. | a low molecular weight (lmw) protein phosphokinase enzyme that binds to actin has been isolated from murine sarcoma virions; this kinase activity is not present in nontransforming murine leukemia viruses. sephadex g-75 gel filtration and affinity chromatography on actin-sepharose conjugates allow a significant level of purification of this enzyme. the enzyme associates with microtubular proteins and inhibits the in vitro polymerization of microtubules. this study represents the first isolation o ... | 1979 | 222479 |
| cycloheximide-dependent reversion of human cells transformed by msv and chemical carcinogen. | the protein synthesis inhibitor cycloheximide, at a concentration of 0.08 microgram per milliliter, induced flat morphology within 24 to 48 hours and low saturation density in human osteosarcoma cells transformed by kirsten murine sarcoma virus (ki-msv) or n-methyl-n' nitro-n-nitrosoguanidine. removal of the protein synthesis inhibitor caused both transformed cells to revert to the transformed phenotype. the demonstration of cell-surface antigens, cross-reacted with antiserums induced by extract ... | 1979 | 223242 |
| murine sarcoma viruses block corticosteroid-induced differentiation of bone marrow preadipocytes associated with long-term in vitro hemopoiesis. | 1979 | 223284 | |
| identification of a sarcoma virus-coded phosphoprotein in nonproducer cells transformed by kirsten or harvey murine sarcoma virus. | a similar protein of 21,000 mw (p21) coded for by harvey or kirsten murine sarcoma virus has been identified in nonproducer cells transformed by these two viruses. antisera prepared from rats bearing tumors induced by syngeneic transplantation of nrk cells transformed by harvey murine sarcoma virus (ha-musv) specifically precipitated the ha-musv p21 from a nonproducer balb/c mouse cell and a nonproducer dog cell transformed by ha-musv. the same antisera also precipitated a similar protein, ki-mu ... | 1979 | 223311 |
| establishment of cell lines from the wild rodent millardia meltada and tests for endogenous virus. | four cell lines were established from the wild rodent, millardia meltada; an untransformed cell line (mm-d), a non-producer (np) cell line transformed with murine sarcoma virus (mm-cl4), and sv40-transformed cell lines (mm-8 mm-663). mm-d is epithelioid and contact-inhibited, whereas mm-cl4, mm-8, and mm-663 are fibroblastic and grow piling up. no endogenous virus was induced in millardia cell lines by a long-term culture with various inducers. millardia cell dna has no nucleotide sequence homol ... | 1979 | 223935 |
| enhancement by asbestos of oncogenesis by moloney murine sarcoma virus in cba mice. | five micrograms of finely ground crocidolite asbestos (uicc standard sample) were injected intraperitoneally into 3-week-old cba mice, together with 10(5) ffu of moloney murine sarcoma virus. altogether 44 out of 61 mice (72.1%) so treated developed palpable intraperitoneal tumours, and half of these died of such tumours within 100 days. the same amount of quartz and carbon similarly administered gave lower tumour incidences, namely, 19.4% (3.2% fatal) and 11.9% (1.5% fatal) respectively. only 1 ... | 1979 | 223995 |
| p21 of kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation. | we have recently described an intracellular protein, p21, in nonproducer cells transformed by either the kirsten (ki-msv) or harvey (ha-msv) strain of murine sarcoma virus (shih et al., virology, in press). the p21 is phosphorylated and has been shown to be coded for by either ki-msv or ha-msv. in this report, we compare the thermal stability of the newly synthesized [35s]methionine-labeled p21 in cells transformed by the wild-type ki-msv or by a mutant of ki-msv (ts 371) which is temperature se ... | 1979 | 225570 |
| orthotopic bone induction at sites of moloney murine sarcoma virus inoculation in mice. | moloney murine sarcoma virus (m-msv) induces at the site of inoculation in newborn and adult mice and rats various types of sarcoma, including osteosarcoma. the induced tumours are fatal in newborn, whereas sarcomas developed in adult animals regress spontaneously. the regression is mediated mainly by a cellular response. we have now demonstrated that the presence of sarcomas induced by m-msv is a powerful stimulus for periosteal osteogenesis around tumour masses. orthotopically induced osteogen ... | 1979 | 225678 |
| cytolytic activity of macrophages isolated from primary murine sarcoma virus (msv)-induced tumors. | macrophages isolated from regressing or progressing tumors induced by murine sarcoma virus (msv) were tested for cytolytic activity in a 18-h 51cr release assay. macrophages from the tumors of mice injected 14 days earlier with stocks of msv-producing regressor or progressor tumors had comparable levels of cytotoxicity. however, macrophages from the progressively growing tumors, 50--65 days after the inoculation with the progressor virus, had lower levels of cytotoxicity than those from the regr ... | 1979 | 226482 |
| thermolabile protein kinase molecules in a temperature-sensitive murine sarcoma virus pseudotype. | murine sarcoma virus-associated protein kinases that bind to actin have been purified by affinity chromatography on actin coupled to sepharose. heat inactivation studies showed the presence of thermolabile enzyme activity in pseudotypes containing a temperature-sensitivity mutant of murine sarcoma virus (msv) but not in two independent wild-type msv pseudotypes. studies with sephadex g-75 column fractions showed that a low molecular weight form, approximately 15,000, is the major thermolabile ki ... | 1979 | 226971 |
| a candidate rat-specific gene product of the kirsten murine sarcoma virus. | 1979 | 227164 | |
| loss of proviral dna sequences in a revertant of kirsten sarcoma virus-transformed murine fibroblasts. | a previously described revertant cell line (k-balb sr1212), derived as a single cell clone from a clonal line of murine fibroblasts (k-balb) transformed by a nonproductive infection with the kirsten strain of murine sarcoma virus, has normal morphology and growth kinetics and, unlike the transformed parent cell line, lacks a sarcoma virus that can be rescued. we report here that this reversion correlates with low to undetectable levels of expression of cellular ki-msv-specific rna and a reductio ... | 1979 | 227995 |
| guanine nucleotide-binding activity as an assay for src protein of rat-derived murine sarcoma viruses. | we have recently identified a 21,000-dalton protein, p21, coded for by kirsten or harvey murine sarcoma virus. on the basis of the results obtained with the p21 of a mutant of kirsten sarcoma virus, temperature sensitive for the maintenance of transformation, we concluded that the p21 was required for the maintenance of transformation induced by either virus. we report herein that when extracts from cells transformed by kirsten or harvey sarcoma virus are incubated with [(3)h]gdp or [alpha-(32)p ... | 1979 | 228288 |
| localization of the asv src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry. | the cellular location of the src gene product (p60src) of the schmidt-ruppin strain of avian sarcoma virus has been determined by electron microscopic immunocytochemistry in schmidt-ruppin asv-transformed nrk cells, and the amount of the protein in different regions of the cell has been quantified. the protein is concentrated on the inner surface of the plasma membrane, particularly under ruffles, and it is highly concentrated on the inner surface of the membrane near junctions connecting adjace ... | 1979 | 228858 |
| in vitro induction of t-lymphocyte-mediated cytotoxicity by infectious murine type c oncornaviruses. | we have developed a system to induce oncornavirus-specific secondary cytotoxic response in vitro. when moloney strain of murine sarcoma virus-immune spleen cells were cultivated with purified infectious moloney murine leukemia virus (m-mulv) or with supernates of tissue culture cells containing infectious virus, a virus-specific secondary cytotoxic response directed against type-specific determinant(s) of m-mulv was generated in vitro, as determined by a 4-h 51cr-release assay. the effector cell ... | 1979 | 229187 |
| heteroduplex analysis of the sequence relationships between the genomes of kirsten and harvey sarcoma viruses, their respective parental murine leukemia viruses, and the rat endogenous 30s rna. | the sequence relations between kirsten murine sarcoma virus (ki-sv), harvey murine sarcoma virus (ha-sv), and a rat endogenous 30s rna were studied by electron microscope heteroduplex analysis. the sequence relationships between the sarcoma viruses and their respective parental murine leukemia viruses (kirsten and moloney murine leukemia viruses), as well as between the two murine leukemia viruses, were also studied. the only observed nonhomology feature of the kirsten murine leukemia virus/molo ... | 1979 | 229250 |
| comparative study of different isolates of murine sarcoma virus. | the rna genomes of a variety of murine sarcoma viruses (msv) were compared by heteroduplex analysis. these viruses included the moloney-derived isolates 124-msv, m1-msv, m3-msv, ht1-msv, and np-msv and also two independent isolates, gazdar msv and 1712-msv. all of these viral genomes exhibited the acquired cellular sequences previously identified in 3124-msv and thought to be responsible for transformation and sarcomagenesis. the location of the acquired cellular sequences within the envelope ge ... | 1979 | 229256 |
| heteroduplex analysis of the rna of clone 3 moloney murine sarcoma virus. | heteroduplex analysis of the rna isolated from purified virions of clone 3 moloney murine sarcoma virus (m-msv) hybridized to cdna's from moloney murine leukemia virus (m-mlv) and clone 124 m-msv shows that the main physical component of clone 3 rna is missing all or most of the 1.5-kilobase (kb) clone 124 m-msv specific sequence denoted beta s (s. hu et al. cell 10:469--477, 1977). this sequence is either deleted in clone 3 rna or substituted by a very short (0.3-kilobase) sequence. in other re ... | 1979 | 229257 |
| cell cycle-dependent chronic infection of human cytomegalovirus in human osteogenic sarcoma cells. | human osteogenic sarcoma cells transformed by murine sarcoma virus (r970) showed restricted growth of human cytomegalovirus (hcmv). virus was attached to the same extent as in human fibroblasts. hcmv growth was blocked at early stages after virus penetration. splitting of infected r970 cultures resulted in infection of all cells. in experiments using synchronized r970 cells it was found that factor(s) associated with the s-phase of the cell cycle might be necessary for establishment of the infec ... | 1979 | 230311 |
| supplemental arginine increases thymic cellularity in normal and murine sarcoma virus-inoculated mice and increases the resistance to murine sarcoma virus tumor. | arginine supplements were given to 6 week old cba mice beginning 3 days prior to inoculation with a murine sarcoma virus, the moloney sarcoma virus (msv). although the basal diet contained 1.8% arginine and was therefore not arginine-deficient, supplementation of the diet and the drinking water with 0.5% arginine hcl reduced tumor incidence, lengthened the latency period, decreased tumor size, and hastened tumor regression. arginine also increased thymic weight and cellularity in normal and in m ... | 1979 | 231121 |
| inhibition of murine sarcoma virus induced transformation in an adenovirus -- nrk system. | cell transformation induced by murine sarcoma virus (msv-m) is significantly inhibited (80--90%) in a clonal line of normal rat kidney (nrk) cells when they are infected with rat cell passaged adenovirus 12 (r-ad12). no inhibition is seen when r-ad12 is added simultaneously with, or 1 1/2 or 24 hr after, msv-m infection, suggesting that inhibition occurs most probably intracellularly. there is also a direct correlation between the extent of focus formation and the concentration of r-ad12 used. c ... | 1979 | 231569 |
| decrese of collagen biosynthesis ability of swiss 3t3 cells transformed with the murine sarcoma virus (msv). | 1979 | 232424 | |
| functional heterogeneity and t cell-dependent activation of macrophages from murine sarcoma virus (msv)-induced tumors. | in studies on the functional activity of macrophages isolated from murine sarcoma virus (msv)-induced tumors, we have found that these cells may suppress immune responses as well as act as effector cells against the tumor. previously, we reported that macrophages from the tumor could inhibit the antitumor response by suppressing proliferation-dependent immune functions. here, we demonstrate that macrophages can also suppress the production of migration inhibition factor (mif) and macrophage acti ... | 1979 | 232622 |
| "sarc" sequence transcription in moloney sarcoma virus-transformed nonproducer cell lines. | (3)h-labeled complementary dna(sarc), complementary to the murine sarcoma virus (msv)-specific portion of the moloney msv (m-msv) genome, was prepared. m-msv-specific rna was then quantitated in the cytoplasm of several m-msv-transformed, non-virus-producing, clonal nih 3t3 cell lines. these lines, designated 71 n clones 5, 6, and 3, have been characterized previously by the degree to which they exhibit transformation properties and transcribe moloney murine leukemia virus-related rna (s. salzbe ... | 1979 | 430602 |
| enhancement of experimental tumors in mice by treatment with concanavalin a. | weanling balb/c mice given injections of 300 micrograms concanavalin a (con a) prior to and at frequent intervals after challenge with moloney murine sarcoma virus (m-musv), 3-methylcholanthrene (mca), or tepc-15 plasmacytoma cells showed an enhancement of tumor induction or development. with the m-musv and mca systems, this enhancement was evidenced by larger tumors and, in the mca system, by more devastating tumors. regression of the m-musv-induced tumors was more prolonged in con a-treated mi ... | 1979 | 480379 |
| prevention of transplantable tumors by adoptive transfer of spleen cells from immunized rats. | fischer 344 rats were specifically hyperimmunized with allogeneic, nonvirus-producing [kirsten murine sarcoma virus (kimsv)] or syngeneic, virus-producing [kimsv (rasheed)] rat tumors. spleen cells taken from these rats adoptively transferred protection against a 100 to 1,000 x rat tumor dose50 cell challenge with several different transplantable rat tumors. protection was obtained with spleen cells after removal of adherent cells and macrophages but not peritoneal cells. the spleen cells were n ... | 1979 | 501086 |
| the transforming gene of moloney murine sarcoma virus. | a cleavage map of the moloney murine sarcoma viral dna was constructed and compared with that of a spontaneously occurring deletion mutant. by restriction enzyme analysis, it was shown that a region encompassing over 40% of the viral information was not essential for transformation or rescue of the deletion mutant. the transforming region was further localised by analysis of the transforming activity in tissue culture of isolated restriction fragments of linear duoble-stranded sarcoma viral dna. ... | 1979 | 503218 |
| immunochemical characterization of tumor-associated surface antigens on a moloney leukemia virus-lymphoma, mbl-2. | tumor-associated surface antigens (tasa) on a moloney leukemia virus (m-mulv)-induced lymphoma, mbl-2, in c57bl/6 mice (b6) were characterized. the surface proteins of mbl-2 cells were selectively radioiodinated and then extracted by nonidet p40. the solubilized materials were then reacted with a variety of antisera: monospecific antisera to murine leukemia viral proteins (anti-gp69/71, anti-p30, anti-p15, anti-p12 and anti-p10), sera from b6 which regressed murine sarcoma tumors induced by muri ... | 1979 | 528073 |
| prevention of spontaneous leukemia in mice by oncornavirus immunization. | c57l, nih, and swr mice were immunized with inactivated gross leukemia virus (glv) and then mated with akr males. their f1 offspring were then immunized with the murine sarcoma virus pseudotype of glv, msv(glv). the concentrations of infectious ecotropic akr virus in tail extracts of immunized mice were 100- to 1,000-fold lower than in non-immunized controls when tested at 30--40 days of age. although viral titers increased slightly with time, the titers remained at least one log10 lower in the ... | 1979 | 216644 |
| radiation induction of endogenous type c virus from mouse cells transformed in vitro by murine sarcoma virus. | cell lines from akr and balb/c mouse embryos were compared for their sensitivity to x-ray induction of endogenous type c virus. k-balb cells, a balb/3t3 cell line nonproductively transformed by kirsten murine sarcoma virus, were found to be sensitive to x-irradiation. at a dose as low as 50 r, x-rays induced virus expression in k-balb cells, and the induction frequency increased with increasing dose of x-rays up to 400 r. among two classes of inducible endogenous viruses carried by k-balb cells, ... | 1979 | 216834 |
| phenotypically distinct target cells for murine sarcoma virus and murine leukemia virus marrow transformation in vitro. | an in vitro hematopoietic microenvironment was established from explained fragments of bone marrow from adult noninbred nih swiss mice with the use of corticosteroid-reconstituted horse serum. infection with kirsten murine sarcoma virus (ki-musv) with either a rauscher murine leukemia virus (r-mulv) or balb:virus-1 helper virus coat reduced proliferation of granulocytic and pluripotent hematopoietic stem cells and produced neoplastic transformation of both macrophages and preadipocytes in the ad ... | 1979 | 216835 |
| suppression of lymphokine production by macrophages infiltrating murine virus-induced tumors. | immune spleen cells from mice injected with murine sarcoma virus (msv) can produce migration inhibition factor (mif) in vitro after stimulation with intact tumor cells. we have now found that this effector function can be regulated by suppressor macrophages present in the tumor. the cells from the tumor (cft) exert a strong suppressive effect even after treatment with anti-thy 1.2 plus complement but lose this capacity if adherent or phagocytic cells are removed, suggesting that the macrophage i ... | 1979 | 314424 |
| in vitro studies of cell-mediated immunity to moloney murine leukemia virus and moloney leukemia-associated surface antigens. | cell-mediated immunity to moloney murine leukemia virus (m-mulv) and to tumor-associated surface antigens of leukemia cells induced by the virus was studied with an in vitro migration inhibition factor assay. spleen cells of c57bl/6n mice at day 14 following inoculation with moloney murine sarcoma virus, produced migration inhibition factor in response to m-mulv. the moloney murine sarcoma virus-immune spleen cells, however, did not respond to other murine type c viruses, to akr and rauscher vir ... | 1979 | 387218 |
| molecular cloning of moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome. | the unintegrated circular dna form of moloney murine sarcoma virus (msv) has been cloned in bacteriophage lambda. discrete deletions in the viral genome were shown to occur during propagation of recombinant phage in escherichia coli. heteroduplex and restriction enzyme analyses indicated the deletion of tandemly repeated sequences within certain of the cloned msv dna inserts. cloned msv dna was used to prepare a probe composed of its acquired cellular (src) sequences, shown previously to be nece ... | 1979 | 392518 |
| suppressor t cells regulate the cytolytic t lymphocyte response to syngeneic tumors induced by murine sarcoma virus (msv) in the mouse. | studies were designed to analyze the immune activities of spleen cells from mice previously injected with murine sarcoma virus (msv) and undergoing the processes of msv tumor growth and rejection. fractionation of msv-primed spleen cells according to cell size by velocity sedimentation at unit gravity showed that msv-specific cytolytic t lymphocytes (ctl) generated in vivo underwent an apparent transition in size from large to small cells as the tumor regressed. the majority of ctl precursors, h ... | 1979 | 156765 |
| a defined subgenomic fragment of in vitro synthesized moloney sarcoma virus dna can induce cell transformation upon transfection. | the longest dna molecules synthesized by endogenous reverse transcription in detergent-permeabilized moloney murine sarcoma virus (mo-msv) virions (clone g8-124) are double-stranded dna molecules of 5,8 kilobase pairs (kbp). this dna species has been purified by sedimentation of total in vitro synthesized mo-msv dna through neutral sucrose gradients. a physical map of the positions of the cleavage sites for a series of restriction endonucleases has been derived for this 5.8 kbp dna. mo-msv dna s ... | 1979 | 84715 |
| inhibition of murine sarcoma virus-induced foci formation by cytidine analogues and other drugs chemotherapeutically effective in human malignancies. | the present report describes an in vitro culture system using the kirsten murine sarcoma virus transformation focus assay to evaluate the antiviral activities of 12 commonly used chemotherapeutically effective drugs. since these drugs are cytotoxic, the plating efficiencies of the cells treated were monitored simultaneously. using this procedure, adriamycin, daunorubicin, bleomycin camptothecin, mithramycin, hydroxyurea, 5-fluorouracil, thioguanine, 9-beta-d-arabinofuranosyladenine, 1-beta-d-ara ... | 1979 | 88425 |
| inhibition of spontaneous transformation of rat embryo cells releasing endogenous type c virus by virus-specific antiserum. | noninbred sprague-dawley rat embryo cell clones predictably undergo transformation after 20-30 in vitro passages following spontaneous release of endogenous rat leukemia virus (ralv). in the presence of ralv-specific antiserum, virus production and infectivity were reduced and transformation was delayed from 6 to 25 weeks. transformation was not associated with an increased expression of kirsten murine sarcoma virus-related src gene rna. | 1979 | 89211 |
| analysis of proteins of mouse sarcoma pseudotype viruses: type-specific radioimmunoassay for ecotropic virus p30's. | murine sarcoma virus pseudotypes were prepared by infection of nonproducer cells (a1-2), which were transformed by the gazdar strain of mouse sarcoma virus, with gross (n-tropic), wn1802b (b-tropic), or moloney (nb-tropic) viruses. the respective host range pseudotype sarcoma viruses were defined by the titration characteristics on cells with the appropriate fv-1 genotype. proteins from virus progeny were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. bands present in bot ... | 1979 | 90164 |
| [biochemical characteristics of a calf leukemia virus in chronically infected cells]. | studied were the conditions of cultivation of flk cells chronically infected with a calf leucosis virus. the gradient values of density were compared to those of the murine sarcoma virus--1.14--1.15 vs, 1.17--1.18/cm3. established were the parameters of the reverse transcriptase reaction for the calf leukosis virus (magnesium-dependent reverse transcriptase). data showed that the calf leucosis virus may not resolutely be referred either to the b- or the the c-type of retroviruses. | 1979 | 92095 |
| suramin: a potent inhibitor of the reverse transcriptase of rna tumor viruses. | suramin--a well-known antitrypanosomal agent--was found to exert a strong inhibitory effect on the rna-directed dna polymerase (reverse transcriptase) activity of several oncornaviruses such as moloney murine leukemia virus, murine rauscher leukemia viruses, moloney murine sarcoma virus and avian myeloblastosis virus. inhibition of enzyme activity was obtained with both endogenous viral rna and (a)n . oligo(dt) as the template-primer. suramin effected a 50% inhibition of the reverse transcriptas ... | 1979 | 92362 |
| detection of cellular immunity to murine sarcoma virus-induced tumors by leukocyte adherence inhibition. | 1979 | 214608 | |
| significance of the proline assay in the study of anti-msv cell-mediated immune reactions. | the cytolysis of 3h-proline-labelled tumour cells growing in monolayer by syngeneic immune lymphocytes has been studied in the murine sarcoma virus (msv) system. results show that the proline assay (pa) is a convenient way to reveal the activity of cytolytic t lymphocytes against fmr-like antigens. using the same effector and target cells, the classical chromium-release test (crt) fails to reveal any cytolytic activity, and the visual microcytotoxicity assay as well as several derived isotopic m ... | 1979 | 215185 |
| protein composition of a defective murine sarcoma virus particle possessing the enveloped type-a morphology. | 1979 | 227155 | |
| pleiotropic phenotype of cultured murine cells resistant to maytansine, vincristine, colchicine, and adriamycin. | a noncloned subline of 3t3fl cells was developed that was resistant to the toxicity of the ansamycin alkaloid maytansine. culture of 3t3fl cells with serially increasing concentrations of maytansine resulted in a cell line resistant to maytansine at concentrations 118-fold higher than concentrations cytotoxic for parental cells. resistant cells (3t3r) exhibited cross-resistance to colchicine, vincristine, adriamycin, and, to a lesser extent, cytochalasin b. studies of binding and uptake of triti ... | 1979 | 224236 |
| tumors induced by murine sarcoma virus contain precursor cells capable of generating tumor-specific cytolytic t lymphocytes. | leukocyte fractions extracted from the tumor mass and the lymphoid organs of c57bl/6 (b6) mice carrying murine sarcoma virus-induced tumors contained primed cytolytic t-lymphocyte (ctl) precursor cells, in addition to active cytotoxic t cells. these leukocyte fractions gave a secondary response when stimulated in vitro with syngeneic tumor cells, generating large numbers of specific ctl. the activity of these ctl (h-2b) was apparently h-2-restricted, because it was ineffective on tumor targets b ... | 1979 | 221614 |
| cytolytic and cytostatic anti-tumor activities of macrophages from mice injected with murine sarcoma virus. | 1979 | 759378 | |
| cytochalasin b-induced multinucleation of murine sarcoma virus-transformed cells. inhibition by sodium butyrate. | 1979 | 215423 | |
| monovalent antibodies directed to transformation-sensitive membrane components inhibit the process of viral transformation. | monovalent antibodies (fab) directed to two classes of transformation-sensitive cell surface components, ganglioside and galactoprotein a (gap a), inhibit the process of oncogenic viral transformation of cells. mouse 3t3 cells infected with murine sarcoma virus were not transformed in terms of morphology change and enhancement of sugar uptake when the infected cells were cultured in the presence of monovalent antibodies directed to gm(1) ganglioside or to gap a. transformation inhibitory activit ... | 1978 | 216010 |
| decreased alkaline phosphatase in cells transformed by rous sarcoma virus. | chick embryo cells transformed by either of two strains of rous sarcoma virus (bryan high titer or schmidt-ruppin) have low levels of alkaline phosphatase activity compared with nontransformed chick embryo cells. essentially no differences in acid phosphatase activity were observed between these transformed and nontransformed cells. a virus mutant, rsv-bh-ta, induces temperature-dependent transformation in infected cells. at 41 degrees, the transformation-nonpermissive temperature, alkaline phos ... | 1978 | 202384 |
| identification of a 30s rna with properties of a defective type c virus in murine cells. | a novel species of 30s rna has been detected in a variety of mouse cell lines. the 30s rna is specifically packaged by helper-independent type c viruses propagated in such cells. nucleic acid hybridization detects no homology between the 30srna and the genomic rna of helper-independent mouse type c viruses. the properties of the 30s rna suggest that it is a defective endogenous mouse type c virus and that it is analogous to a previously described class of defective endogenous rat type c virus, w ... | 1978 | 202730 |
| glucocorticoids induce focus formation and increase sarcoma viral expression in a mink cell line that contains a murine sarcoma viral genome. | dexamethasone (3 x 10(-10) to 3 x 10(-6) m) induced foci of morphologically transformed cells in a small proportion of a mink cell line that contains the moloney murine sarcoma viral genome (s+l-). the induction was glucocorticoid specific, since other steroids with glucocorticoid activity (prednisolone, cortisol, and aldosterone) induced foci with an efficiency that paralleled their glucocorticoid activity, and steroids lacking glucocorticoid activity (17b-estradiol, testosterone, and progester ... | 1978 | 202733 |
| immunological study of two stocks of moloney sarcoma virus producing regressor and progressor tumors in c57bl/6 mice. | the immune response to progressor murine sarcoma virus (p-musv)2 and regressor murine sarcoma virus (r-musv) was analyzed by the radioisotopic footpad assay (fpa) for delayed hypersensitivity (dh) and by the membrane immunofluorescence assay for anti-tumor antibodies. in mice injected with p-musv, the footpad response remained at a low level for 7 weeks, while membrane immunofluorescence antibody titers reached their maximum levels after 5 weeks. by contrast, in mice injected with r-musv, the fo ... | 1978 | 203543 |
| in situ embedding method of cells grown in beem capsules for immunoelectron microscopic studies of oncornaviruses. | a technique of in situ embedding of cells grown in beem capsules has been devised for immunoelectron microscopic studies of oncornaviruses. as compared to other immunoelectron microscopic procedures, this technique is less time and reagent-consuming. the quality and specificity of this method were tested on well-characterized mouse mammary tumor virus (type b virus) and murine sarcoma virus (type c virus particles). this method gave good results in labeling of the virus particles with ferritin o ... | 1978 | 203626 |
| effect of the fv-1 locus in vivo: host range pseudotypes of murine sarcoma virus. | fv-1-specific host-range pseudotypes of murine sarcoma virus (musv) were developed by rescue from nonproducer cells with n- or b-tropic leukemia viruses. the musv(b) and musv(n) pseudotype viruses were tested in vitro and were restricted specifically by the fv-1 gene locus. when the pseudo-type viruses were tested in vivo in mice of specific fv-1 geno-types, tumor induction was completely restricted in nonpermissive animals, including athymic nude mice, whereas tumors grew progressively in permi ... | 1978 | 204797 |
| electron microscopic studies of intracisternal virus particles in soehner-dmochowski murine sarcoma virus-induced bone tumors of new zealand black rats. | soehner-dmochowski murine sarcoma virus (moloney)-induced bone tumors of new zealand black rats carry two morphologically different types of virus particles, namely, extracellular type c and intracisternal virus particles, which have thus far not been reported. these two types of virus particles have also been observed in the tissue culture cells derived from normal prostate tissues of a/dm and balb/c/dm mice after inoculation of cell-free extracts of these bone tumors. the intracisternal virus ... | 1978 | 205348 |
| studies with the baboon endogenous virus and its pseudotype of murine sarcoma virus in marmosets. | three marmoset species (saguinus oedipus oedipus, s. fuscicollis, callithrix jacchus) failed to show evidence of infection or disease following inoculation of baboon endogenous type-c virus (baev). cells infected with a kirsten murine sarcoma-baev pseudotype (msv[baev]) produced a progressive fibrosarcoma in s. o. oedipus and tumors in s. fuscicollis and c. jacchus which regressed. tumor formation is believed to be due to growth of inoculated, virus-infected cells and not transformation of marmo ... | 1978 | 205851 |
| fbj osteosarcoma virus in tissue culture. iii. isolation and characterization of non-virus-producing fbj-transformed cells. | hamster and rat cell lines have been established that have been transformed by fbj murine sarcoma virus (fbj-musv) but that do not produce virus. the hamster cell line originated from an osteosarcoma that appeared in a hamster inoculated at birth with an extract of a cfno1 mouse fbj-osteosarcoma. the rat cell line was obtained by transferring the fbj-musv genome to normal rat kidney cells in the absence of the fbj type c virus (fbj-mulv), which, usually in high concentration, accompanies the fbj ... | 1978 | 206718 |
| effects of culture conditions on the growth and differentiation of transformed rat adrenocortical cells. | normal rat adrenocortical cells grow in vitro in two states of differentiation, depending on culture conditions. under one set of conditions, they proliferate and are fibroblast shaped, with some myoid cell characteristics and with limited steroidogenic activity. under alternate conditions they do not replicate and are epithelial-like and similar to adrenocortical secretory cells structurally and steroidogenically. the effects of oncogenic transformation on these cellular responses to culture va ... | 1978 | 207413 |
| the "sarcoma-specific" region of moloney murine sarcoma virus 124. | labeled, purified 30s rna from moloney murine sarcoma virus was annealed to an excess of moloney murine leukemia virus complementary dna. upon treatment of the resulting dna.rna hybrids with rnase h followed by sucrose gradient sedimentation, and undigested 18s rna molecule was recovered. this rna molecule was shown to represent the "sarcoma-specific" region of the virus. the unintegrated linear dna provirus of murine sarcoma virus 124 was isolated from newly infected cells and a physical map of ... | 1978 | 208071 |
| in vitro isolation of stable rat sarcoma viruses. | a sprague-dawley (sd-1) rat embryo culture, at low passage level, released an endogenous ecotropic type c virus (sd-ralv) and after about 20 further passages it underwent spontaneous transformation. the sd-ralv, released from the transformed cells, did not cause rapid transformation of other rat embryo cells. however, when the transformed cells were repeatedly cocultivated with three different chemically transformed and serially transplanted rat tumor cell lines (sarcoma, carcinoma, and hepatoma ... | 1978 | 208081 |
| transformation of indian muntjac cells by murine and avian sarcoma viruses. | indian muntjac cells were efficiently transformed by murine sarcoma virus (msv) and avian sarcoma viruses (asv). when colony formation of the infected cells was examined in soft agar, many colonies were formed by the asv-injected cells but no colony was seen in the msv-infected cells. the asv-transformed cell clones differed among the clones in morphology, presence of inducible asv genome, and karyotypes. | 1978 | 208907 |
| two different murine sarcoma virus isolates have homologous genome sequences: implication for their origin. | 1978 | 210574 | |
| comparison of the genomic organization of kirsten and harvey sarcoma viruses. | current studies were undertaken to compare the genomes of kirsten murine sarcoma virus (ki-musv), harvey murine sarcoma virus (ha-musv), and the replication-defective endogenous rat virus to understand the function of these viral rnas. genome organization and sequence homology were studied by fingerprinting large rnase t1-resistant oligonucleotides and by cross-protecting homologous oligonucleotides against rnase a and t1 digestion with complementary dna prepared from each of the other viral rna ... | 1978 | 211254 |
| growth factors from murine sarcoma virus-transformed cells. | murine sarcoma virus-transformed mouse fibroblasts produce polypeptide growth factors and release them into serum-free medium. these factors stimulate cells to divide in monolayer cultures and also to form colonies that grow progressively soft agar. three major peaks of activity are seen, with apparent molecular weights of 25,000, 12,000, and 7000. the sarcoma growth factors are heat-stable, trypsin-sensitive, and active in nanogram quantities when tested for growth stimulation of untransformed ... | 1978 | 211512 |
| the effect of mouse interferon on the transformation of nih/3t3 mouse cells by murine sarcoma virus. | 1978 | 211721 | |
| nucleases and adenosine 3',5'-cyclic monophosphate phosphodiesterase activities in murine sarcoma virus (moloney)-infected mice. | to provide information on the role of nucleases in oncogenic virus infection, the activities of 3'-nucleotide phosphodiesterase (3'-npdase), 5'-nucleotide phosphodiesterase (5'-npdase), acid deoxyribonuclease (dnase ii), and 3',5'-cyclic amp phosphodiesterase (campdase) in spleen extracts of murine sarcoma virus-infected c57bl/6 inbred mice were studied. at the peak of tumor growth and of the cell-mediated cytotoxic response (cmc) against tumor-associated antigens, 3'-npdase, 5'-npdase, and dnas ... | 1978 | 212566 |
| viral gene expression in murine sarcoma virus(murine leukemia virus)-infected cells. | nih 3t3 cells infected with moloney murine sarcoma virus (murine leukemia virus) produce virions which contain about 99% murine sarcoma virus rna and 1% murine leukemia virus rna. this report describes experiments which measured intracellular concentrations of proviral dna and rna transcripts for each of the viruses. we found that three to four copies of proviral dna from each virus were integrated into the cellular dna. measurements of rna specific for each of the genomes by hybridization to sp ... | 1978 | 212592 |
| phosphorylation of chromatin- and ribosome-associated proteins in cells transformed by adenovirus, murine sarcoma virus, and methylcholanthrene. | endogenous protein phosphorylation in chromatin and ribosomes of monkey, mouse, and rat cells transformed by dna, rna tumor viruses, and a chemical carcinogen revealed the association of a protein of approximately 90,000 daltons (90k), which is highly phosphorylated in vitro. peptide map analysis showed that the 90k proteins associated with these organelles of various transformed cells are similar irrespective of the species of cells and transforming agents. this species of protein could not be ... | 1978 | 212594 |
| structural markers on core protein p30 of murine leukemia virus: functional correlation with fv-1 tropism. | tryptic peptide maps from more than 50 isolates of murine leukemia virus (mulv) have shown that, in general, the structure of core protein p30 is highly conserved. however, a structurally variable region of p30 has been identified that is functionally associated with fv-1 tropism. on the basis of this structural variability, mulv strains can be classified as b-tropic, n-tropic, xenotropic, and/or as being derived from wild mice. certain xenotropic viruses have a p30 like that of b-tropic mulv an ... | 1978 | 212738 |
| stimulatory effect of immunization on tumor induction by moloney murine sarcoma virus. | adult mice were immunized with varying doses of inactivated moloney murine leukemia virus (m-mulv). eight weeks after immunization, mice were challenged with a dose of moloney murine sarcoma virus (m-musv) that could induce tumors in approximately 50% of normal animals. mice immunized with high doses of m-mulv (10(10) particles) had significantly decreased tumor incidences, whereas mice immunized with low doses of m-mulv (10(2) particles) had significnatly increased tumor incidences compared to ... | 1978 | 213613 |
| ornithine decarboxylase activity in cells acutely and chronically transformed by murine sarcoma virus. | 1978 | 213779 | |
| [effect of sodium butyrate on the interferon sensitivity of normal or cells transformed by murine sarcoma virus]. | sodium butyrate enhances the sensitivity of msv transformed murine cells to interferon, but does not influence the response of normal cells. sodium butyrate might act in transformed cells by restoring the different elements of the cytoskeletal system. | 1978 | 214252 |
| a replication-defective variant of moloney murine leukemia virus. i. biological characterization. | we have studied the virus produced by a clone, termed 8a, that was isolated from a culture of murine sarcoma virus-transformed mouse cells after superinfection with moloney murine leukemia virus (mulv-m). clone 8a produced high levels of type c virus particles, but only a low titer of infectious murine sarcoma virus and almost no infectious mulv. when fresh cultures of mouse cells were infected with undiluted clone 8a culture fluids, they released no detectable pogeny virus for several weeks aft ... | 1978 | 74421 |
| further evidence for deletion of envelope glycoprotein (gp69/71) sequences in formation of moloney-murine sarcoma virus. | moloney-murine sarcoma virus (s+l- strain of m-msv) has been nonproductively cloned in murine and non-murine host cells (s+l- cells) and the expression of moloney leukaemia virus (m-mulv) 30000 mol. wt. core protein (p30) and envelope glycoprotein (gp69/71) were studied by radioimmunoassay. antigenic determinants of the m-mulv p30 were associated with the sarcoma virus genome in these non-productively transformed cell clones studied, while the determinants of m-mulv gp69/71 were not. the absence ... | 1978 | 80447 |
| secondary in vitro generation of cytolytic t-lymphocytes (ctl's) in the murine sarcoma virus system. virus-specific ctl induction across the h-2 barrier. | following in vitro stimulation of murine sarcoma virus moloney isolate (m-musv)-immune spleen cells with syngeneic antigenically related moloney leukemia cells, highly efficient cytotoxic t-lymphocytes (ctl's) were generated. the cytotoxic effect was directed only against h-2-compatible target cells bearing m-musv tumor-associated antigens (taa). however, in a cold target competition assay a weak but detectable capacity to block ctl activity was also obtained when allogeneic moloney leukemia cel ... | 1978 | 80457 |
| chemical transformation of human revertant cells induced by murine sarcoma virus. | a human revertant cell line, derived from non-producer human osteosarcoma cells (np/khos) induced by kirsten murine sarcoma virus, was treated in vitro with various levels of polycyclic aromatic hydrocarbons (3-methylcholanthrene, 7,12-dimethylbenz(alpha)anthracene, and benzo(alpha)pyrene [bp] or dimethyl sulfoxide (control). cells treated only with 3-methylcholanthrene and 7,12-dimethylbenz(alpha)anthracene underwent morphological alteration in vitro, and produced tumors when injected into nih ... | 1978 | 415010 |
| revertants of mouse cells transformed by murine sarcoma virus. v. loss of msv-specific nucleotide sequences from cellular rna. | 1978 | 217157 | |
| activity of 10-chloro-5-(2-dimethylaminoethyl)-7h-indolo [2,3-c]-quinolin-6(5h)-one hydrochloride against experimental tumors in mice and rats. | 10-chloro-5-(2-dimethylaminoethyl)-7h-indolo [2,3-c]-quinolin-6(5h)-one hydrochloride (ciq) was shown to exert significant antitumor activity against the ehrlich carcinoma and sarcoma 180 transplantable tumors in mice by the intraperitoneal (ip) or oral (po) routes and when incorporated into diet. a solid tumor induced in balb/c mice by the subcutaneous (sc) implantation of nonproducer murine sarcoma virus-transformed balb/3t3 cells was also inhibited by ciq after ip or po treatment but there wa ... | 1978 | 579626 |
| anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. identification of the anti-viral activity as interferon and characterization of the human effector lymphocyte subpopulation. | a viral inhibitor(s) is released in the supernate of mixed cultures containing human or mouse lymphocytes and cells from certain lines. the inhibitor is active against a variety of unrelated viruses and is a protein that is not toxic for cells. it does not inactivate viruses directly, but inhibits viral replication through an intracellular mechanism that involves synthesis by the cells of both rna and protein. these characteristics identify the inhibitor as an interferon. the anti-viral activity ... | 1978 | 650155 |
| in situ fc receptor-bearing cells in two murine tumors. i. isolation and identification. | the in situ inflammatory response was studied in 2 different tumors, the t1699 mammary adenocarcinoma in inbred dba/2 mice and a sarcoma induced in inbred c57bl/6 mice by murine sarcoma virus. the presence of fc receptors (fcr's) on infiltrating host cells was determined by rosette formation with antibody-coated sheep red blood cells (ea). differences in fcr-binding activity among various cells were detected with the use of ea prepared with a range of antibody concentrations. cells bearing fcr r ... | 1978 | 650702 |
| effects of hydroxyurea on murine type c virus-specific dna synthesis in newly infected cells. | cell transformation and replication of the rauscher pseudotype of moloney murine sarcoma virus in mouse embryo fibroblasts were inhibited by hydroxyurea within a critical time period of 30 to 90 min postinfection. in cells infected by rauscher leukemia virus, treatment with 1mm hydroxyurea during the critical time period resulted in the accumulation of minus-strand dna (molecular weight, 3 x 10(6)) in association with the parental viral genoma rna. this 5 to 6 x 10(6) dalton rna:dna hybrid was f ... | 1978 | 657143 |
| protective effect of murine sarcoma virus-superinfected mouse tumor cells against outgrowth of corresponding noninfected tumor. | cultured lines of three newly established methylcholanthrene-induced tumors, mbk and mbl in cba and mc57m in c57bl mice, and two mammary tumors, sbfnhc in cba and s3w in asw mice, were superinfected in vitro with moloney sarcoma or leukemia virus (msv, mlv). after superinfection, they expressed the moloney virus-determined cell surface antigen (mcsa) and murine c-type viral p30 antigen, and produced nb-tropic c-type virus. the virus-infected tumors became more rejectable in normally susceptible ... | 1978 | 705875 |
| comparative immunological studies on cryosurgery and surgical operation using moloney murine sarcoma virus-induced primary tumors in balb/c mice. | the effect of cryosurgery and surgical operation on moloney murine sarcoma virus-induced primary tumors in the treated and untreated groups of balb/c mice was compared in terms of tumor growth, cumulative mortality, lymphocyte-mediated cytotoxicity, proliferative response of lymphocytes, and humoral antibody formation. the results indicate that cryosurgical treatment showed both in vivo and in vitro effects; (i) tumor growth and cumulative mortality in mice treated by cryosurgery were significan ... | 1978 | 710802 |
| epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (egf) receptors and the effect of mouse sarcoma virus transformation. | fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, nrk. these clones were studied for their ability to bind epidermal growth factor (egf), susceptibility to transformation by mouse sarcoma virus (msv), and alteration in egf binding upon sarcoma virus transformation. the epithelioid clones bound much more egf than the fibroblastic clones; scatchard plots on two of these clones, one epithelioid and one fibroblastic, showed that the higher egf binding (1.3 x 10( ... | 1978 | 304450 |
| murine sarcoma virus (msv)-induced tumors in mice. i. distribution of msv-immune cytolytic t lymphocytes in vivo. | quantitative studies using a 51cr release assay were performed to analyse the time-course of appearance and specificity of cytolytic cells in c57bl/6 mice inoculated with moloney murine sarcoma virus (msv). various lymphoid organs were studied including spleen, mesenteric lymph nodes (mln), lymph nodes regional to the msv-induced tumor (rln), and peripheral blood. furthermore, the kinetics of appearance of cytolytic cells within the msv-induced tumor was determined. in agreement with previous st ... | 1977 | 300075 |
| t and b lymphocyte migration into syngeneic tumors. | the migration of splenic t and b lymphocytes into syngeneic tumors undergoing immunologic rejection was investigates. spleen cells were obtained from normal balc/c mice or balb/c mice bearing tumors induced by murine sarcoma virus (msv). either whole spleen cells or immunoabsorbent purified t and b cells were radiolabeled with sodium chromate-51 and injected i.v. into normal or msv inducted-tumor bearing syngeneic recipients. twenty-four hours later the recipient mice were sacrificed and radioac ... | 1977 | 300384 |
| standardization of the chromium-51 release, cell-mediated cytotoxicity assay: cryopreservation of mouse effector and target cells. | utilizing controlled cryopreservation techniques, we were able to standardize the 51cr release cytotoxicity assay and thereby ensured reliable comparisons between results obtained on different days. optimal conditions for freezing of both effector and target cells were quite similar. dimethyl sulfoxide (dmso) at a concentration of 7.5-10.0% was employed as the cryoprotective agent and cells were frozen at the rate of -1 degrees c/minute. the handling procedures for the cells before and after fre ... | 1977 | 839557 |
| a heteroduplex study of the sequence relationships between the rnas of m-msv and m-mlv. | the regions of sequence homology between the rna genomes of a murine sarcoma virus (clone 124 moloney-msv) and its parental helper virus (clone 3 moloney-murine leukemia virus (m-mlv)) have been mapped. long complementary dna transcripts of the m-mlv rna were hybridized to m-msv rna, and the structures of the hybrids were observed in the electron microscope. beginning at the 5' end, the two rnas are homologous for a region of length 2.25 kb (kilobases). in the next region, of length approximatel ... | 1977 | 844103 |
| augmentation of natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic target cells. | the levels of natural cell-mediated cytotoxicity against tumor cells in young balb/c and balb/c nude mice could be augmented by inoculation of a variety of mouse tumor cells or of mouse thymocytes. in older mice with low levels of spontaneous cytotoxic reactivity, inoculation of tumor cells led to rapid appearance of cytotoxicity. this augmented cytotoxicity reached a peak 3 days after inoculation and then declined rapidly. the specificity of the augmented cytotoxicity appeared to be the same as ... | 1977 | 844921 |
| defective friend spleen focus-forming virus: interfering properties and isolation free from standard leukemia-inducing helper virus. | defective friend spleen focus-forming virus (sffv) is able to interfere with the ability of its naturally occurring leukemia-inducing helper virus (llv-f) to induce xc plaque formation in several different strains of mouse embryo cells. this interference has been observed by using two different sffv preparations, one contained in an nb-tropic stock of friend virus (fv) complex, and the second present in a c57bl-adapted strain of fv complex containing an associated b-tropic llv-f helper. the llv- ... | 1977 | 904028 |
| phenotypic markers in human skin fibroblasts as possible diagnostic indices of hereditary adenomatosis of the colon and rectum. | hereditary adenomatosis of the colon and rectum (acr) and its gardner's syndrome variant, an autosomal dominant trait, indicate a propensity for neoplasia. the present study describes the growth abnormalities of cultured human skin fibroblasts derived from normal-appearing cutaneous biopsies of acr genotypes and a portion of the clinically asymptomatic acr progeny, first filial generation, and their differential susceptibility to transformation by kirsten murine sarcoma virus. these skin fibrobl ... | 1977 | 922693 |
| enhancement of tumor induction in rats with moloney murine sarcoma virus by a "new" method based on direct injection into fetuses. | undiluted, fivefold-diluted, and 25-fold-diluted doses of a stock of moloney murine sarcoma virus were injected directly, in a volume of 0.025 ml, into the backs of fetal sprague-dawley rats by laparotomy through the uterine wall at 18 days of gestation. during the first 8 weeks after birth the young responded to the virus with remarkably high but dose-dependent incidences of neoplasms. when a one-fifth dilution of the virus preparation was inoculated at fetal ages 16, 18, and 20 days, the incid ... | 1977 | 264591 |
| intratibial moloney sarcoma virus-induced osteosarcoma in the rat: tumor incidence and pathologic evaluation. | osteosarcomas were induced in approximately 80% of young new zealand black rats by the intratibial inoculation of moloney murine sarcoma virus from day 1 to day 5 after birth. the neoplasms were composed of a spectrum of well-differentiated to poorly differentiated osteoblasts, osteocytes, and osteoclasts. budding of c-type viral particles was associated with tumor induction. compared to rats inoculated on day 1 after birth, rats inoculated at 4 days of age developed consistently more osteoproli ... | 1977 | 264594 |
| cleavage map of linear mouse sarcoma virus dna. | proviral dna transcribed from the rna of moloney murine sarcoma virus was isolated from newly infected cells. three forms of viral dna were observed: (i) a linear double-stranded form of 3.4 x 10(6) daltons which constituted the major viral dna species in the cell, and is thought to be a complete transcript (monomer) of viral rna; (ii) a fast-sedimenting viral dna bigger than the monomeric unit which can be either integrated provirls or concatamers; and (iii) covalently closed circles of monomer ... | 1977 | 264682 |
| characterization of human cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine. | human osteosarcoma (hos) clonal cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) were characterized, and compared to non-producer hos cells transformed by kirsten murine sarcoma virus (ki-msv). the mnng- and virus-transformed cells grew in the aggregate form above an agar base, grew in soft agar, and had a high fibrinolytic activity. when inoculated into nude mice, all the chemically or virally altered cells produced tumors or tumor nodules. when transplanted into ats-tr ... | 1977 | 265298 |
| pathology of neoplasms and other lesions induced in rats with moloney murine sarcoma virus. | moloney murine sarcoma virus (m-msv) was injected directly into the fetuses of sprague-dawley rats during the late stage of gestation and into the neonates within 24 hours after birth. ninety rats developed 188 neoplastic lesions during the 8-week period of observation. nearly all of the neoplasms were of mesenchymal derivation. sixty percent of these neoplasms revealed more complex histologic features than those previously reported for neoplasms induced in rodents with m-msv and were designated ... | 1977 | 266356 |
| interruption of oncornavirus replication by modified rifamycin antibiotics. | thirteen rifamycin sv derivatives containing 3'-alkylaminomethyl substituents fail to inhibit the activities of the simian sarcoma virus type 1 dna polymerase, and of cellular dna, rna, and poly(a) polymerases prepared from nih swiss mouse embryos. these compounds show a range in their toxicities for nih swiss mouse 3t3 cells and in their capacities to inhibit production of foci of morphologically altered cells by murine sarcoma virus (msv). three compounds--the n-methyl-n-hydroxyethylaminomethy ... | 1977 | 81643 |
| genetics of murine sarcoma virus (msv)--induced tumors in akr mice: evidence that late progressing and early regressing tumors are controlled by different gents. | the genetics of late appearing msv tumors showing a progressive growth pattern in akr mice was investigated. the late msv tumor response in f1 hybrids depended on the genetic background of the non-akr parent. within the 4-month observation period following virus injection, (cba x akr) f1, (dba/2 x akr)f1, and (nih x akr)f1 developed progressing msv tumors, which exhibited latency and growth behavior comparable to that seen in akr mice, (balb x akr)f1, (b6 x akr)f1, and (b10br x akr)f1 mice did n ... | 1977 | 66212 |