Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| comparison of bioassay responses to the potential fungal biopesticide metarhizium anisopliae in rhipicephalus(boophilus) microplus and lucilia cuprina. | quantal response bioassays were conducted with cattle ticks and sheep blowflies with three different isolates of metarhizium anisopliae and different methods of inoculation. ticks were either topically dosed with 2μl or immersed in the conidial preparations. blowflies were either topically dosed with 2μl of the conidial preparation or fed on conidia mixed with sugar. probit analyses were carried out on the mortality data to compare the virulence of these isolates to ticks and blowflies and look ... | 2011 | 22127104 |
| natural occurrence of lethal aspergillosis in the cattle tick rhipicephalus (boophilus) microplus (acari:ixodidae). | summarythe purpose of this study was to describe an unreported entomopathogenic fungus that naturally infects the cattle tick rhipicephalus (boophilus) microplus (acari: ixodidae). engorged female ticks, showed symptoms of fungal infection after controlled tick infestation of cattle. infected ticks developed a distinctive dark colour, a pale mould grew over the cuticle and the ticks eventually died covered with fungal conidiophores. the responsible fungus was isolated and cultured on mycological ... | 2011 | 22018391 |
| tick cell lines for study of crimean-congo hemorrhagic fever virus and other arboviruses. | abstract continuous cell lines derived from many of the vectors of tick-borne arboviruses of medical and veterinary importance are now available. their role as tools in arbovirus research to date is reviewed and their potential application in studies of tick cell responses to virus infection is explored, by comparison with recent progress in understanding mosquito immunity to arbovirus infection. a preliminary study of propagation of the human pathogen crimean-congo hemorrhagic fever virus (cc ... | 2011 | 21955214 |
| partial characterization of an atypical family i inorganic pyrophosphatase from cattle tick rhipicephalus (boophilus) microplus. | the present paper presents the partial characterization of a family i inorganic pyrophosphatase from the hard tick rhipicephalus (boophilus) microplus (bmppase). the bmppase gene was cloned from the tick embryo and sequenced. the deduced amino acid sequence shared high similarity with other eukaryotic ppases, on the other hand, bmppase presented some cysteine residues non-conserved in other groups. this pyrophosphatase is inhibited by ca(2+), and the inhibition is antagonized by mg(2+), suggesti ... | 2011 | 22001703 |
| abc transporter efflux pumps: a defense mechanism against ivermectin in rhipicephalus (boophilus) microplus. | atp-binding cassette (abc) transporters are efflux transporters found in all organisms. these proteins are responsible for pumping xenobiotic and endogenous metabolites through extra- and intracellular membranes, thereby reducing cellular concentrations of toxic compounds. abc transporters have been associated with drug resistance in several nematodes and parasitic arthropods. here, the ability of abc transporter inhibitors to enhance ivermectin (ivm) sensitivity was tested in larvae and adult f ... | 2011 | 22036897 |
| bartonellae in animals and vectors in new caledonia. | bartonellae are gram-negative facultative intracellular alpha-proteobacteria from the family bartonellaceae. the natural history of bartonellae consists of a reservoir/host, which is a vertebrate with chronic intravascular infection with sustained bacteremia, and a vector (usually an arthropod) that transfers the bacteria from the reservoir to a susceptible yet uninfected host. in order to reveal the sources and reservoirs of bartonella infection in animals and vectors in new caledonia, we colle ... | 2011 | 22018646 |
| acaricidal effect of an isolate from hoslundia opposita vahl against amblyomma variegatum (acari: ixodidae). | hoslundia opposita vahl. (lamiaceae), a common local shrub in ghana, is traditionally known not only for its pharmacological benefits but also for its insecticidal properties. its acaricidal property, however, has not been investigated. | 2011 | 22022167 |
| Perceptions of milk producers from Divinópolis, Minas Gerais, regarding Rhipicephalus (Boophilus) microplus control. | Semi-systematized interviews were conducted with 100 dairy cattle producers in the municipality of Divinópolis, Minas Gerais, with the aim of ascertaining their perceptions regarding the importance of Rhipicephalus (Boophilus) microplus and of combating it. Content analysis was performed and the frequency distribution of each of the variables was used to construct profiles of the producers interviewed. The production losses caused by ticks were perceived incompletely by the producers, who were u ... | 2011 | 22166383 |
| evolution of the max and mlx networks in animals. | transcription factors (tfs) are essential for the regulation of gene expression and often form emergent complexes to perform vital roles in cellular processes. in this paper, we focus on the parallel max and mlx networks of tfs because of their critical involvement in cell cycle regulation, proliferation, growth, metabolism, and apoptosis. a basic-helix-loop-helix-zipper (bhlhz) domain mediates the competitive protein dimerization and dna binding among max and mlx network members to form a compl ... | 2011 | 21859806 |
| characterizing the host and symbiont proteomes in the association between the bobtail squid, euprymna scolopes, and the bacterium, vibrio fischeri. | the beneficial symbiosis between the hawaiian bobtail squid, euprymna scolopes, and the bioluminescent bacterium, vibrio fischeri, provides a unique opportunity to study host/microbe interactions within a natural microenvironment. colonization of the squid light organ by v. fischeri begins a lifelong association with a regulated daily rhythm. each morning the host expels an exudate from the light organ consisting of 95% of the symbiont population in addition to host hemocytes and shed epithelial ... | 2011 | 21998678 |
| Acaricidal activity of aqueous extract and synthesized silver nanoparticles from Manilkara zapota against Rhipicephalus (Boophilus) microplus. | Traditional parasite control is primarily based on the use of chemical acaricides, which unfortunately have many negative side effects. The aim of the present study was to evaluate the effect of plant synthesized silver nanoparticles (AgNPs) using aqueous leaf extract of Manilkara zapota to control Rhipicephalus (Boophilus) microplus. The synthesized AgNPs were characterized by UV-vis spectrum, scanning electron microscopy (SEM), Fourier transform infrared and X-ray diffraction. The UV-vis spect ... | 2011 | 21906765 |
| Distribution of Rhipicephalus (Boophilus) microplus and Rhipicephalus (Boophilus) annulatus (Acari: Ixodidae) infestations detected in the United States along the Texas/Mexico border. | Species identification and global positioning system coordinates of infestations of cattle fever ticks, Rhipicephalus (Boophilus) annulatus (Say) and Rhipicephalus (Boophilus) microplus (Canestrini), were determined for 790 specimens submitted to the National Veterinary Services Laboratory between 1 October 1999 and 30 September 2010. Cattle fever tick specimens obtained by personnel of the United States Department of Agriculture-Animal and Plant Health Inspection Service-Cattle Fever Tick Eradi ... | 2011 | 21845935 |
| the ecology, genetic diversity and phylogeny of huaiyangshan virus in china. | surveys were carried out to better understand the tick vector ecology and genetic diversity of huaiyangshan virus (hysv) in both endemic and non-endemic regions. haemaphysalis longicornis ticks were dominant in endemic regions, while rhipicephalus microplus is more abundant in non-endemic regions. hysv rna was found in human and both tick species with more prevalence in h. longicornis and lesser in r. microplus. phylogenetic analyses indicate that hysv is a novel species of the genus phlebovirus ... | 2011 | 22190717 |
| recombinant mid gut antigen (bm95) as a vaccine against indian rhiphicephalus haemaphysaloides in bos indicus cattle. | in the present study, we report for the first time the efficacy of recombinant bm95 mid gut antigen isolated from an argentinean strain of rhipicephalus microplus strain a in controlling the tick infestations in india. the synthetic gene for bm95 optimized for expression in yeast was obtained and used to generate yeast transformants expressing bm95 which was purified to apparent homogeneity. liquid chromatography-mass spectrometry analysis of the purified protein confirmed its identity as bm95. ... | 2011 | 20580387 |
| heterozygote deficiencies in parasite populations: an evaluation of interrelated hypotheses in the raccoon tick, ixodes texanus. | population genetics is increasingly being used to study the biology of parasites at the scales of both the host (infrapopulation, ip) and host population (component population, cp). in this study we tested three mechanistic hypotheses that could explain deviations from hardy-weinberg equilibrium (hwe) expectations due to heterozygote deficits (hds) at the cp scale in raccoon ticks (ixodes texanus; n=718) collected from raccoons (procyon lotor; n=91) and genotyped at 11 microsatellite loci. these ... | 2011 | 20606689 |
| differential transcription of two highly divergent gut-expressed bm86 antigen gene homologues in the tick rhipicephalus appendiculatus (acari: ixodida). | the transcriptional control of gene expression is not well documented in the arthropoda. we describe transcriptional analysis of two exceptionally divergent homologues (ra86) of the bm86 gut antigen from rhipicephalus appendiculatus. bm86 forms the basis of a commercial vaccine for the control of rhipicephalus (boophilus) microplus. the r. appendiculatus ra86 proteins contain 654 and 693 amino acids, with only 80% amino acid sequence identity. reverse-transcription pcr of gut cdna showed transcr ... | 2011 | 20854482 |
| female ixodid ticks grow endocuticle during the rapid phase of engorgement. | lees (proc zool soc lond 121:759-772, 1952) concluded that the ixodid tick ixodes ricinus grows endocuticle during the slow but not during the rapid, phase of engorgement, a conclusion supported by andersen and roepstorff (insect biochem mol biol 35:1181-1188, 2005) for the same species. in this study analysis of dimensional data and cuticle weight measurements from female ixodid ticks (amblyomma hebraeum) were used to test this hypothesis. both approaches showed that endocuticle growth continue ... | 2011 | 20711799 |
| multiple transcripts encode glucose 6-phosphate dehydrogenase in the southern cattle tick, rhipicephalus (boophilus) microplus. | glucose 6-phosphate dehydrogenase (g6pdh) is an enzyme that plays a critical role in the production of nadph. here we describe the identification of four transcripts (g6pdh-a, -b, -c, and -d) that putatively encode the enzyme in the southern cattle tick, rhipicephalus (boophilus) microplus. the genomic dna that is spliced to produce g6pdh-a and -b is 8,600-9,000 bases in length and comprises 12 exons. comparison of the r. microplus g6pdh gene structure with those available from insects and mamma ... | 2011 | 20711800 |
| rhipicephalus (boophilus) microplus: a most successful invasive tick species in west-africa. | the cattle tick rhipicephalus (boophilus) microplus is known to be a highly reproductive and efficient vector of babesia bovis, two characters which make this tick a threat to livestock keeping in many continents. the authors identified this tick in ivory coast, west africa, in 2007, and hypothesized the spread to be minimal, as this tick was not observed in previous years. to determine the extent of its distribution and to a lesser extent the possible impact of the tick on the livelihoods of iv ... | 2011 | 20711801 |
| efficiency of sulphur in garlic extract and non-sulphur homeopathy in the control of the cattle tick rhipicephalus (boophilus) microplus. | the objective of the present work was to evaluate the efficacy of a non-sulphur-based homeopathic preparation and a sulphur-containing natural product derived from allium sativum (linnaeus) against infestation by the cattle tick rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae). a total of 24 crossbred calves (7 : 8 holstein : zebu), aged 6-8 months and maintained in individual stables under tick-free conditions, were divided into three groups. group 1 (control group) received n ... | 2011 | 20819153 |
| cysteine proteases from bloodfeeding arthropod ectoparasites. | cysteine proteases have been discovered in various bloodfeeding ectoparasites. here, we assemble the available information about the function of these peptidases and reveal their role in hematophagy and parasite development. while most of the data shed light on key proteolytic events that play a role in arthropod physiology, we also report on the association of cysteine proteases with arthropod vectorial capacity. with emphasis on ticks, specifically ixodes ricinus, we finally propose a model ab ... | 2011 | 21660665 |
| [identification of haemaphysalis longicornis and rhipicephalus microplus]. | to establish a method combined morphology and molecular marker for identifying haemaphysalis longicornis and rhipicephalus microplus. | 2011 | 21781482 |
| generation, analysis and functional annotation of expressed sequence tags from the ectoparasitic mite psoroptes ovis. | abstract: background: sheep scab is caused by psoroptes ovis and is arguably the most important ectoparasitic disease affecting sheep in the uk. the disease is highly contagious and causes and considerable pruritis and irritation and is therefore a major welfare concern. current methods of treatment are unsustainable and in order to elucidate novel methods of disease control a more comprehensive understanding of the parasite is required. to date, no full genomic dna sequence or large scale trans ... | 2011 | 21781297 |
| enhancing the acaricide effect of thymol on larvae of the cattle tick rhipicephalus microplus (acari: ixodidae) by solubilization in ethanol. | the aim of the present work was to propose a new method of solubilizing thymol for use as an acaricide and to evaluate the resulting product's efficacy on rhipicephalus microplus larvae. two experiments were performed, both employing the larval packet test to evaluate the potential of the solutions formulated. in the first experiment, r. microplus larvae were exposed to three new formulations: formulation 1-distilled water (70%) + ethanol (30%) + thymol (10.0 mg/ml); formulation 2-distilled wate ... | 2011 | 21779863 |
| the complexity of rhipicephalus (boophilus) microplus genome characterised through detailed analysis of two bac clones. | abstract: background: rhipicephalus (boophilus) microplus (rmi) a major cattle ectoparasite and tick borne disease vector, impacts on animal welfare and industry productivity. in arthropod research there is an absence of a complete chelicerate genome, which includes ticks, mites, spiders, scorpions and crustaceans. model arthropod genomes such as drosophila and anopheles are too taxonomically distant for a reference in tick genomic sequence analysis. this study focuses on the de-novo assembly of ... | 2011 | 21777481 |
| a campaign to eradicate bovine babesiosis from new caledonia. | in december 2007, babesia bovis was introduced to new caledonia through the importation of cattle that had been vaccinated with a live tick fever (babesiosis and anaplasmosis) vaccine. although the tick rhipicephalus (boophilus) microplus is common in new caledonia, the territory had previously been free of tick-borne diseases of cattle. this paper describes the initial extent of the outbreak, the measures and rationale for disease control, and the progress to date of the eradication campaign. i ... | 2011 | 21771538 |
| acaricidal activity of the essential oil from tetradenia riparia (lamiaceae) on the cattle tick rhipicephalus (boophilus) microplus (acari; ixodidae). | tetradenia riparia (lamiaceae) is a well-known herbal medicine with a variety of useful properties, including its acaricidal effect. this experiment was carried out to study the bioacaricidal activity of t. riparia essential oil (eo) against engorged females of rhipicephalus (boophilus) microplus (acari; ixodidae). for this purpose, nine serial concentrations (12.50%, 6.25%, 3.75%, 1.80%, 0.90%, 0.45%, 0.22%, 0.11%, and 0.056% w/v) of t. riparia were used for the adult immersion test (ait). for ... | 2011 | 21762693 |
| detection of bacteria related to candidatus midichloria mitochondrii in tick cell lines. | many ticks have been shown to be infected with intracellular bacteria. one of these bacteria is candidatus midichloria mitochondrii which is the only characterized bacterium that has the ability to invade the mitochondria within ovarian cells and consume them without any effect on the female tick's reproduction. in the present study, eight cell lines derived from the ticks ixodes ricinus, ixodes scapularis, rhipicephalus (boophilus) microplus, and rhipicephalus (boophilus) decoloratus were exami ... | 2011 | 21748354 |
| a mitochondrial membrane exopolyphosphatase is modulated by, and plays a role in, the energy metabolism of hard tick rhipicephalus (boophilus) microplus embryos. | the physiological roles of polyphosphates (polyp) recently found in arthropod mitochondria remain obscure. here, the relationship between the mitochondrial membrane exopolyphosphatase (ppx) and the energy metabolism of hard tick rhipicephalus microplus embryos are investigated. mitochondrial respiration was activated by adenosine diphosphate using polyp as the only source of inorganic phosphate (p(i)) and this activation was much greater using polyp(3) than polyp(15). after mitochondrial subfrac ... | 2011 | 21747692 |
| in vitro efficacy of plant extracts and synthesized substances on rhipicephalus (boophilus) microplus (acari: ixodidae). | herbal drugs have been widely evaluated as an alternative method of parasite control, aiming to slow development of resistance and obtain low-cost biodegradable parasiticides. this study evaluated the in vitro efficacy on rhipicephalus (boophilus) microplus of extracts from carapa guianensis seed oil, cymbopogon martinii and cymbopogon schoenanthus leaf essential oil, and piper tuberculatum leaf crude extract and similar synthesized substances. in the immersion test, engorged females were evalua ... | 2011 | 21695568 |
| first report of a rhipicephalus microplus tick population multi-resistant to acaricides and ivermectin in the mexican tropics. | we document the presence of a rhipicephalus microplus tick population resistant to acaricides (organophosphates (op), synthetic pyrethroids (sp), amitraz) and macrocyclic lactones (ml) (ivermectin). engorged females of r. microplus were collected from a cattle farm in veracruz, mexico, to evaluate acaricide and ivermectin resistance. the modified larval packet test (lpt) was used to detect op (chlorpiriphos and diazinon) and sp (flumethrin, deltamethrin and cypermethrin) resistance and the larva ... | 2011 | 21824728 |
| perspectives for the use of plant extracts to control the cattle tick rhipicephalus (boophilus) microplus. | the evolution of resistance of rhipicephalus (boophilus) microplus to synthetic acaricides has given rise to the need for new scientific investigations on alternative ways to control this tick. in this regard, various studies on plants have been developed in an attempt to find extracts with acaricidal properties. evaluations on plant extracts for controlling r. (b.) microplus have grown intensely over the last decade. there are many advantages from using plant extracts: for example, they can be ... | 2011 | 21722481 |
| acaricide resistance of rhipicephalus (boophilus) microplus in state of mato grosso do sul, brazil. | this study was conducted to obtain an epidemiological view of acaricide resistance in populations of rhipicephalus (boophilus) microplus in the state of mato grosso do sul. twenty-four tick samples were collected from municipalities in the state where farmers had reported concerns about resistance to or failure of tick control. these ticks were subjected to in vitro resistance detection assays using the adult immersion test (ait). the efficacy of alpha-cypermethrin, cypermethrin and amitraz trea ... | 2011 | 21722487 |
| molecular characterization of novel sulfotransferases from the tick, ixodes scapularis. | ixodes scapularis, commonly known as the blacklegged or deer tick, is the main vector of lyme disease in the united states. recent progress in transcriptome research has uncovered hundreds of different proteins expressed in the salivary glands of hard ticks, the majority of which have no known function, and include many novel protein families. we recently identified transcripts coding for two putative cytosolic sulfotransferases in these ticks which recognized phenolic monoamines as their substr ... | 2011 | 21708020 |
| strategic control of cattle ticks: milk producers' perceptions. | in order to evaluate milk producers' knowledge about tests on tick sensitivity to acaricides, efficiency tests and strategic control, questionnaires were delivered to 670 producers. from these, 163 responses were received. these producers had sent ingurgitated female ticks to be subjected to tests on tick sensitivity to acaricides at embrapa between 2001 and 2005. most of the completed questionnaires came from the state of minas gerais, brazil. after descriptive analysis and correlation of varia ... | 2011 | 21722490 |
| isolation and characterisation of acaricidal and larvicidal novel compound (2s,5r,6r)-2-hydroxy-3,5,6-trimethyloctan-4-one from streptomyces sp. against blood-sucking parasites. | the aim of the present study was to assess the acaricidal and larvicidal property of marine actinobacterial compound (2s,5r,6r)-2-hydroxy-3,5,6-trimethyloctan-4-one extracted and isolated from streptomyces sp. vitddk3 tested against the larvae of rhipicephalus (boophilus) microplus canestrini (acari: ixodidae), anopheles subpictus grassi and culex quinquefasciatus say (diptera: culicidae). the isolate was taxonomically characterised, identified and designated as streptomyces sp. vitddk3. the cru ... | 2011 | 21698506 |
| evolutionary conserved micrornas are ubiquitously expressed compared to tick-specific mirnas in the cattle tick rhipicephalus (boophilus) microplus. | micrornas (mirnas) are small non-coding rnas that act as regulators of gene expression in eukaryotes modulating a large diversity of biological processes. the discovery of mirnas has provided new opportunities to understand the biology of a number of species. the cattle tick, rhipicephalus (boophilus) microplus, causes significant economic losses in cattle production worldwide and this drives us to further understand their biology so that effective control measures can be developed. to be able t ... | 2011 | 21699734 |
| attenuation of virulence in an apicomplexan hemoparasite results in reduced genome diversity at the population level. | abstract: | 2011 | 21838895 |
| gene expression evidence for off-target effects caused by rna interference-mediated gene silencing of ubiquitin-63e in the cattle tick rhipicephalus microplus. | knowledge of cattle tick (rhipicephalus (boophilus) microplus; acari: ixodidae) molecular and cellular pathways has been hampered by the lack of an annotated genome. in addition, most of the tick expressed sequence tags (ests) available to date consist of ôê+50% unassigned sequences without predicted functions. the most common approach to address this has been the application of rna interference (rnai) methods to investigate genes and their pathways. this approach has been widely adopted in tick ... | 2011 | 21712043 |
| an elisa to detect serum antibodies to the salivary gland toxin of ixodes holocyclus neumann in dogs and rodents. | the ixodes holocyclus tick causes paralysis in up to 10,000 companion and domestic animals each year in australia. treatment requires the removal of the parasite and the administration of a commercial tick antiserum that is prepared from hyperimmune dogs. each batch of this serum is initially tested for toxin-neutralising potency in a mouse bioassay that is expensive, time consuming, and subjective. with the aim of developing a rapid in vitro assay to replace the bioassay, we used a partially pu ... | 2011 | 21687655 |
| antigen fingerprinting of polyclonal antibodies raised in immunized chickens with tick total proteins: a reservoir for the discovery of novel antigens. | identification of tick-protective antigens remains the limiting step in vaccine development. the authors have generated several b cell epitope candidates by fingerprinting rhipicephalus (boophilus) microplus proteins that were characterized through bioselection of random peptide phage display libraries against polyclonal antibodies antitick proteins. from 280 clones selected and sequenced, 107 distinct reactive clones were validated by dot-blot assays. eight consensus motifs were generated, and ... | 2011 | 21844329 |
| rhipicephalus (boophilus) microplus: expression and characterization of bm86-cg in pichia pastoris. | the cattle tick rhipicephalus (boophilus) microplus is responsible for great economic losses. it is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. vaccines may help control this parasite, thereby reducing tick pesticide use. in this light, we performed subcloning of the gene of the protein bm86-gc, the homologue protein that currently forms the basis of vaccines (gavac(tm) and tickgard(plus)) that have been developed against cattle ticks. the ... | 2011 | 21722483 |
| a new in vitro test to evaluate the resistance level against acaricides of the cattle tick, rhipicephalus (boophilus) microplus. | in this article we present a new bioassay to assess the resistance status of ticks to acaricides. the larval tarsal test (ltt) is a sensitive, highly time-effective in vitro test. it allows the investigation of a large number of compounds and doses on the cattle tick rhipicephalus (boophilus) microplus in a short period of time. the ability of the ltt to assess the lethal concentration at 50% mortality (lc(50)) and resistance ratios (rrs) of a susceptible and a resistant r. microplus strain was ... | 2011 | 21741175 |
| An intensive search for promising fungal biological control agents of ticks, particularly Rhipicephalus microplus. | Entomopathogenic fungi have been investigated worldwide as promising biological control agents of the cattle tick Rhipicephalus microplus. The current study evaluates the virulence of several fungal isolates to R. microplus larva in the laboratory as part of an effort to identify isolates with promise for effective biocontrol of R. microplus in the field. Sixty fungal isolates, encompassing 5 Beauveria spp. and 1 Engyodontium albus (=Beauveria alba), were included in this study. In addition to b ... | 2011 | 21705145 |
| Scabies mite peritrophins are potential targets of human host innate immunity. | Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families located in the mite gut as crucial factors for host-parasite interactions. Among these multiple proteins inhibit human complement, presumably to avoid complement-mediated damage of gut ep ... | 2011 | 21980545 |
| Acaricidal effect of essential oils from Lippia graveolens (Lamiales: Verbenaceae), Rosmarinus officinalis (Lamiales: Lamiaceae), and Allium sativum (Liliales: Liliaceae) against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). | Acaricidal effects of three essential oils extracted from Mexican oregano leaves (Lippia graveolens Kunth), rosemary leaves (Rosmarinus officinalis L.), and garlic bulbs (Allium sativum L.) on 10-d-old Rhipicephalus (Boophilus) microplus (Canestrini) tick larvae were evaluated by using the larval packet test bioassay. Serial dilutions of the three essential oils were tested from a starting concentration of 20 to 1.25%. Results showed that both Mexican oregano and garlic essential oils had very s ... | 2011 | 21845941 |
| Acaricidal, insecticidal, and larvicidal efficacy of fruit peel aqueous extract of Annona squamosa and its compounds against blood-feeding parasites. | Plant products may be alternative sources of parasitic control agents, since they constitute a rich source of bioactive compounds that are eco-friendly and nontoxic products. The plant extracts are good and safe alternatives due to their low toxicity to mammals and easy biodegradability. In the present study, fruit peel aqueous extract of Annona squamosa (Annonaceae) extracted by immersion method exhibited adulticidal activity against Haemaphysalis bispinosa (Acarina: Ixodidae) and the hematopha ... | 2011 | 22006187 |
| Effects of microplusin, a copper-chelating antimicrobial peptide, against Cryptococcus neoformans. | Microplusin is an antimicrobial peptide isolated from the cattle tick Rhipicephalus (Boophilus) microplus. Its copper-chelating ability is putatively responsible for its bacteriostatic activity against Micrococcus luteus as microplusin inhibits respiration in this species, which is a copper-dependent process. Microplusin is also active against Cryptococcus neoformans (MIC(50) = 0.09 µM), the etiologic agent of cryptococcosis. Here, we show that microplusin is fungistatic to C. neoformans and thi ... | 2011 | 22092765 |
| The effects of flumethrin (Bayticol® pour-on) on European ticks exposed to treated hairs of cattle and sheep. | Tick infestations in cattle and sheep pose serious health problems when agents of diseases are transmitted. In addition, blood feeding of ticks induces enormous economic losses due to reduced weight gain of infested animals. The present study was designed to investigate the effects of exposure to hairs clipped from cattle and sheep treated with flumethrin (Bayticol®) on European ticks. The dose used was 10 ml/100 kg body weight for both animal species. At intervals of 7 days (days 7, 14, 21, 28 ... | 2011 | 22205348 |
| distinctive amino acid composition profiles in salivary proteins of the tick ixodes scapularis. | bioinformatic analysis of the amino acid composition of proteins of the tick ixodes scapularis showed that, in comparison to other secreted proteins, salivary proteins in general have higher frequencies of polar residues and lower frequencies of the non-polar residues leucine and valine. computer prediction of linear b-cell epitopes showed that polar residues were associated with the presence of high-quality epitopes and that tick salivary proteins included significantly more proteins with predi ... | 2011 | 22108016 |
| An insight into the sialotranscriptome and proteome of the coarse bontlegged tick, Hyalomma marginatum rufipes. | Ticks are mites specialized in acquiring blood from vertebrates as their sole source of food and are important disease vectors to humans and animals. Among the specializations required for this peculiar diet, ticks evolved a sophisticated salivary potion that can disarm their host's hemostasis, inflammation, and immune reactions. Previous transcriptome analysis of tick salivary proteins has revealed many new protein families indicative of fast evolution, possibly due to host immune pressure. The ... | 2011 | 21851864 |
| Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) HP88 for biological control of Rhipicephalus microplus (Acari: Ixodidae): The effect of different exposure times of engorged females to the nematodes. | The aim of this study was to assess the influence of different exposure times of engorged female the Rhipicephalus microplus to infective juveniles of Heterorhabditis bacteriophora isolate HP88. The engorged females were divided into seven groups (six treatments and one control) of 20 ticks each with statistically similar average weights (p>0.05) and exposed to concentrations of 300 nematodes/tick for periods of 3, 6, 12, 24, 48 and 72h. The following biological parameters were monitored: pre-ov ... | 2011 | 22093907 |
| applicability of in vitro bioassays for the diagnosis of ivermectin resistance in rhipicephalus microplus (acari: ixodidae). | the applicability of laboratory bioassays to diagnose ivermectin (ivm) resistance in rhipicephalus microplus was evaluated. adult immersion tests (aits), larval immersion tests (lits) and larval packet tests (lpts) were performed to characterise the effects of ivermectin toxicity on adults and larvae of a susceptible reference strain. the ait was determined to be a reasonable assay but requires a large number of individuals to attain interpretable results. the lit and lpt were validated with an ... | 2011 | 21978742 |
| CattleTickBase: An integrated Internet-based bioinformatics resource for Rhipicephalus (Boophilus) microplus. | The Rhipicephalus microplus genome is large and complex in structure, making it difficult to assemble a genome sequence and costly to resource the required bioinformatics. In light of this, a consortium of international collaborators was formed to pool resources to begin sequencing this genome. We have acquired and assembled genomic DNA into contigs that represent over 1.8Gigabase pairs of DNA from gene-enriched regions of the R. microplus genome. We also have several datasets containing transcr ... | 2011 | 22178513 |
| Cutaneous hypersensitivity test to evaluate phage display anti-tick borne vaccine antigen candidates. | Early experiments performed by our group with the phage display technique revealed the potential for using epitope-displaying phages (mimotopes) as a tool for tick antigen discovery. Thus, as a preliminary study, inflammatory reactions induced by phage display tick-borne candidates were investigated by using the cutaneous hypersensitivity test. The profile of selected Rhipicephalus microplus mimotopes was assessed on tick field-exposed cattle and our data indicated a pattern similar to immediate ... | 2011 | 21945847 |
| detection and identification of ehrlichia species in rhipicephalus (boophilus) microplus ticks in cattle from xiamen, china. | abstract a total of 289 rhipicephalus (boophilus) microplus canestrini (acari: ixodidae) collected from cattle in xiamen city of fujian province, southeastern china, were tested for ehrlichial agents by nested polymerase chain reaction assay. twenty-seven (9.3%) ticks from seven cattle were found positive for ehrlichia. the polymerase chain reaction products from positive samples were sequenced and found identical with each other. the 1458-bp nucleotide sequence of ehrlichial 16s rrna gene was o ... | 2011 | 20420536 |
| cross immunity with haemaphysalis longicornis glutathione s-transferase reduces an experimental rhipicephalus (boophilus) microplus infestation. | recombinant glutathione s-transferase of haemaphysalis longicornis (rgst-hl) was expressed in escherichia coli, purified by affinity chromatography and used in the immunization of cattle. western blot analysis showed positive antibody response in cattle immunized with rgst-hl. the tests also showed that immunized bovine sera recognize native rhipicephalus microplus proteins in different tissue extracts. furthermore, the vaccine potential of rgst-hl was investigated against infestation of herefor ... | 2011 | 20619263 |
| ticks on pastures and on two breeds of cattle in the eastern cape province, south africa. | many studies on the population dynamics of questing ticks on pastures and of parasitic ticks on cattle have been conducted. few, however, have attempted to link the two in a single study. this study aimed to assess the population dynamics of questing ixodid ticks on pastures and of adult ticks on two breeds of cattle with different levels of susceptibility to tick infestation on the same pastures. between january 2005 and december 2009 questing ixodid ticks were collected monthly from natural pa ... | 2011 | 23327215 |
| coendangered hard-ticks: threatened or threatening? | the overwhelming majority of animal conservation projects are focused on vertebrates, despite most of the species on earth being invertebrates. estimates state that about half of all named species of invertebrates are parasitic in at least one stage of their development. the dilemma of viewing parasites as biodiversity or pest has been discussed by several authors. however, ticks were omitted. the latest taxonomic synopses of non-fossil ixodidae consider valid 700 species. though, how many of th ... | 2011 | 21554736 |
| first report of ctenocephalides felis felis infestation of buffalo calves in punjab, india. | two female buffalo calves of approximately 3 months of age from hoshiarpur and gurdaspur districts of punjab state, india were found to be moderately infested with the cat flea ctenocephalides felis felis. the calves were apparently healthy with no clinical signs and skin lesions. the calves were also found to be simultaneously infested with ixodid tick (rhipicephalus (boophilus) microplus). infestations of buffalo calves by c. felis felis is reported for the first time. | 2011 | 23024513 |
| biocontrol of pigeon tick argas reflexus (acari: argasidae) by entomopathogenic fungus metarhizium anisopliae (ascomycota: hypocreales). | the pigeon tick argas reflexus is a pathogen-transmitting soft tick that typically feeds on pigeons, but can also attack humans causing local and systemic reactions. chemical control is made difficult due to environmental contamination and resistance development. as a result, there is much interest in increasing the role of other strategies like biological control. in this study, the efficacy of three strains (v245, 685 and 715c) of entomopathogenic fungus metarhizium anisopliae for biological c ... | 2011 | 24031777 |
| neural control of salivary glands in ixodid ticks. | studies of tick salivary glands (sgs) and their components have produced a number of interesting discoveries over the last four decades. however, the precise neural and physiological mechanisms controlling sg secretion remain enigmatic. major studies of sg control have identified and characterized many pharmacological and biological compounds that activate salivary secretion, including dopamine (da), octopamine, γ-aminobutyric acid (gaba), ergot alkaloids, pilocarpine (pc), and their pharmacolog ... | 2011 | 22119563 |
| genomic identification of a putative circadian system in the cladoceran crustacean daphnia pulex. | essentially nothing is known about the molecular underpinnings of crustacean circadian clocks. the genome of daphnia pulex, the only crustacean genome available for public use, provides a unique resource for identifying putative circadian proteins in this species. here, the daphnia genome was mined for putative circadian protein genes using drosophila melanogaster queries. the sequences of core clock (e.g. clock, cycle, period, timeless and cryptochrome 2), clock input (cryptochrome 1) and clock ... | 2011 | 21798832 |
| brown spider (loxosceles genus) venom toxins: tools for biological purposes. | venomous animals use their venoms as tools for defense or predation. these venoms are complex mixtures, mainly enriched of proteic toxins or peptides with several, and different, biological activities. in general, spider venom is rich in biologically active molecules that are useful in experimental protocols for pharmacology, biochemistry, cell biology and immunology, as well as putative tools for biotechnology and industries. spider venoms have recently garnered much attention from several rese ... | 2011 | 22069711 |
| acidocalcisomes as calcium- and polyphosphate-storage compartments during embryogenesis of the insect rhodnius prolixus stahl. | the yolk of insect eggs is a cellular domain specialized in the storage of reserve components for embryo development. the reserve macromolecules are stored in different organelles and their interactions with the embryo cells are mostly unknown. acidocalcisomes are lysosome-related organelles characterized by their acidic nature, high electron density and large content of polyphosphate bound to several cations. in this work, we report the presence of acidocalcisome-like organelles in eggs of the ... | 2011 | 22096545 |
| p2x receptor homologs in basal fungi. | 2011 | 21887491 | |
| effects of passages through a suitable host of the fungus, metarhizium anisopliae, on the virulence of acaricide-susceptible and resistant strains of the tick, rhipicephalus microplus. | the aim of this work was to assess the virulence of strain m379 of the fungus, metarhizium anisopliae (metchnikoff) sorokin (hypocreales: clavicipitaceae) after different passages through a suitable host and at different concentrations for the control of both acaricide-susceptible and resistant strains of the tick, rhipicephalus (formerly boophilus) microplus canestrini (ixodida:ixodidae) in vitro. the highest value of lc50 for the susceptible strain corresponded to zero passage with 7.68 × 107 ... | 2011 | 26983168 |
| expression patterns of neural genes in euperipatoides kanangrensis suggest divergent evolution of onychophoran and euarthropod neurogenesis. | one of the controversial debates on euarthropod relationships centers on the question as to whether insects, crustaceans, and myriapods (mandibulata) share a common ancestor or whether myriapods group with the chelicerates (myriochelata). the debate was stimulated recently by studies in chelicerates and myriapods that show that neural precursor groups (npgs) segregate from the neuroectoderm generating the nervous system, whereas in insects and crustaceans the nervous tissue is produced by stem c ... | 2010 | 21149708 |
| heme and blood-feeding parasites: friends or foes? | hemoparasites, like malaria and schistosomes, are constantly faced with the challenges of storing and detoxifying large quantities of heme, released from their catabolism of host erythrocytes. heme is an essential prosthetic group that forms the reactive core of numerous hemoproteins with diverse biological functions. however, due to its reactive nature, it is also a potentially toxic molecule. thus, the acquisition and detoxification of heme is likely to be paramount for the survival and establ ... | 2010 | 21087517 |
| diversity of phylogenetic information according to the locus and the taxonomic level: an example from a parasitic mesostigmatid mite genus. | molecular markers for cladistic analyses may perform differently according to the taxonomic group considered and the historical level under investigation. here we evaluate the phylogenetic potential of five different markers for resolving evolutionary relationships within the ectoparasitic genus dermanyssus at the species level, and their ability to address questions about the evolution of specialization. coi provided 9-18% divergence between species (up to 9% within species), 16s rrna 10-16% (u ... | 2010 | 20480038 |
| inverse agonist and neutral antagonist actions of synthetic compounds at an insect 5-ht1 receptor. | 5-hydroxytryptamine (5-ht) has been shown to control and modulate many physiological and behavioural functions in insects. in this study, we report the cloning and pharmacological properties of a 5-ht(1) receptor of an insect model for neurobiology, physiology and pharmacology. | 2010 | 20233210 |
| gene-knockdown in the honey bee mite varroa destructor by a non-invasive approach: studies on a glutathione s-transferase. | the parasitic mite varroa destructor is considered the major pest of the european honey bee (apis mellifera) and responsible for declines in honey bee populations worldwide. exploiting the full potential of gene sequences becoming available for v. destructor requires adaptation of modern molecular biology approaches to this non-model organism. using a mu-class glutathione s-transferase (vdgst-mu1) as a candidate gene we investigated the feasibility of gene knockdown in v. destructor by double-st ... | 2010 | 20712880 |
| germ band retraction as a landmark in glucose metabolism during aedes aegypti embryogenesis. | the mosquito a. aegypti is vector of dengue and other viruses. new methods of vector control are needed and can be achieved by a better understanding of the life cycle of this insect. embryogenesis is a part of a. aegypty life cycle that is poorly understood. in insects in general and in mosquitoes in particular energetic metabolism is well studied during oogenesis, when the oocyte exhibits fast growth, accumulating carbohydrates, lipids and proteins that will meet the regulatory and metabolic n ... | 2010 | 20184739 |
| vaccine efficacy of bm86 ortholog of h. a. anatolicum, rhaa86 expressed in prokaryotic expression system. | the use of tick vaccine in controlling ticks and tick borne diseases has been proved effective in integrated tick management format. for the control of h. a. anatolicum, bm86 ortholog of h. a. anatolicum was cloned and expressed as fusion protein in e. coli as e. coli-pethaa86. the molecular weight of the rhaa86 was 97 kda with a 19 kda fusion tag of thioredoxin protein. the expressed protein was characterized immunologically and vaccine efficacy was evaluated. after 120 hours of challenge, only ... | 2010 | 20721331 |
| r2 dynamics in triops cancriformis (bosc, 1801) (crustacea, branchiopoda, notostraca): turnover rate and 28s concerted evolution. | the r2 retrotransposon is here characterized in bisexual populations of the european crustacean triops cancriformis. the isolated element matches well with the general aspects of the r2 family and it is highly differentiated from that of the congeneric north american triops longicaudatus. the analysis of 5' truncations indicates that r2 dynamics in t. cancriformis populations show a high turnover rate as observed in drosophila simulans. for the first time in the literature, though, individuals h ... | 2010 | 20628416 |
| thrombin inhibitors from different animals. | venous and arterial thromboembolic diseases are still the most frequent causes of death and disability in high-income countries. clinical anticoagulants are inhibitors of enzymes involved in the coagulation pathway, such as thrombin and factor x(a). thrombin is a key enzyme of blood coagulation system, activating the platelets, converting the fibrinogen to the fibrin net, and amplifying its self-generation by the activation of factors v, viii, and xi. thrombin has long been a target for the deve ... | 2010 | 20976270 |
| molecular evolution and the role of oxidative stress in the expansion and functional diversification of cytosolic glutathione transferases. | cytosolic glutathione transferases (cgst) are a large group of ubiquitous enzymes involved in detoxification and are well known for their undesired side effects during chemotherapy. in this work we have performed thorough phylogenetic analyses to understand the various aspects of the evolution and functional diversification of cgsts. furthermore, we assessed plausible correlations between gene duplication and substrate specificity of gene paralogs in humans and selected species, notably in mamma ... | 2010 | 20843339 |
| invasion of mosquito salivary glands by malaria parasites: prerequisites and defense strategies. | the interplay between vector and pathogen is essential for vector-borne disease transmission. dissecting the molecular basis of refractoriness of some vectors may pave the way to novel disease control mechanisms. a pathogen often needs to overcome several physical barriers, such as the peritrophic matrix, midgut epithelium and salivary glands. additionally, the arthropod vector elicites immune responses that can severely limit transmission success. one important step in the transmission of most ... | 2010 | 20621627 |
| comparative microarray analysis of rhipicephalus (boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on bos indicus and bos taurus cattle. | rhipicephalus (boophilus) microplus is an obligate blood feeder which is host specific to cattle. existing knowledge pertaining to the host or host breed effects on tick transcript expression profiles during the tick - host interaction is poor. | 2010 | 20637126 |
| bm86 homologues and novel ataq proteins with multiple epidermal growth factor (egf)-like domains from hard and soft ticks. | tick control on livestock relies principally on the use of acaricides but the development of acaricide resistance and concerns for environmental pollution underscore the need for alternative control methods, for instance through the use of anti-tick vaccines. two commercial vaccines based on the recombinant bm86 protein from rhipicephalus (boophilus) microplus ticks were developed. partial protection of the bm86 vaccine against other rhipicephalus (boophilus) and hyalomma tick species suggests t ... | 2010 | 20647015 |
| an insight into the sialotranscriptome of the brown dog tick, rhipicephalus sanguineus. | rhipicephalus sanguineus, known as the brown dog tick, is a common ectoparasite of domestic dogs and can be found worldwide. r.sanguineus is recognized as the primary vector of the etiological agent of canine monocytic ehrlichiosis and canine babesiosis. here we present the first description of a r. sanguineus salivary gland transcriptome by the production and analysis of 2,034 expressed sequence tags (est) from two cdna libraries, one consctructed using mrna from dissected salivary glands from ... | 2010 | 20650005 |
| endophytic and entomopathogenic strains of beauveria sp to control the bovine tick rhipicephalus (boophilus) microplus. | pathogenicity of strains of the entomopathogenic fungus beauveria bassiana and endophytic strains of beauveria sp against the bovine tick rhipicephalus (boophilus) microplus was tested in laboratory bioassays and under field conditions. suspensions containing 10(5), 10(7) and 10(9) conidia/ml were prepared of each fungal strain for laboratory bioassays. the ticks were maintained at 28 degrees c, 90 +/- 5% relative humidity, and the following variables were evaluated: initial female weight, egg w ... | 2010 | 20662157 |
| characterization of proteinases from the midgut of rhipicephalus (boophilus) microplus involved in the generation of antimicrobial peptides. | abstract: | 2010 | 20663211 |
| amblyomma imitator ticks as vectors of rickettsia rickettsii, mexico. | real-time pcr of amblyomma imitator tick egg masses obtained in nuevo leon state, mexico, identified a rickettsia species. sequence analyses of 17-kd common antigen and outer membrane protein a and b gene fragments showed to it to be r. rickettsii, which suggested a potential new vector for this bacterium. | 2010 | 20678325 |
| pyrethroid resistance in aedes aegypti from grand cayman. | the grand cayman population of aedes aegypti is highly resistant to ddt and pyrethroid insecticides. glutathione transferase, cytochrome p450, and esterase levels were increased in the grand cayman population relative to a susceptible laboratory strain, but synergist studies did not implicate elevated insecticide detoxification as a major cause of resistance. the role of target site resistance was therefore investigated. two substitutions in the voltage-gated sodium channel were identified, v101 ... | 2010 | 20682868 |
| acaricide resistance mechanisms in the two-spotted spider mite tetranychus urticae and other important acari: a review. | the two-spotted spider mite tetranychus urticae koch is one of the economically most important pests in a wide range of outdoor and protected crops worldwide. its control has been and still is largely based on the use of insecticides and acaricides. however, due to its short life cycle, abundant progeny and arrhenotokous reproduction, it is able to develop resistance to these compounds very rapidly. as a consequence, it has the dubious reputation to be the"most resistant species" in terms of the ... | 2010 | 20685616 |
| diagnoses of fipronil resistance in brazilian cattle ticks (rhipicephalus (boophilus) microplus) using in vitro larval bioassays. | fipronil is a phenylpyrazolic insecticide that is widely used in agriculture and has been recently used to control the cattle tick, rhipicephalus (boophilus) microplus. because of the serious problems associated with resistance to the available acaricides, this product has been used as an important alternative to control acaricide-resistant ticks. the objective of this work was to analyse the fipronil sensitivity of ticks that were collected from farms with a history of fipronil use by larval bi ... | 2010 | 20688434 |
| differential expression of genes in salivary glands of male rhipicephalus (boophilus)microplus in response to infection with anaplasma marginale. | bovine anaplasmosis, caused by the rickettsial tick-borne pathogen anaplasma marginale (rickettsiales: anaplasmataceae), is vectored by rhipicephalus (boophilus)microplus in many tropical and subtropical regions of the world. a. marginale undergoes a complex developmental cycle in ticks which results in infection of salivary glands from where the pathogen is transmitted to cattle. in previous studies, we reported modification of gene expression in dermacentor variabilis and cultured ixodes scapu ... | 2010 | 20298599 |
| laboratory and field evaluation of metarhizium anisopliae (deuteromycotina: hyphomycetes) for the control of rhipicephalus microplus (acari: ixodidae) in the mexican tropics. | the aim of the present study was to evaluate the effect of metarhizium anisopliae to control rhipicephalus microplus under laboratory and field conditions (larvae on vegetation) in the mexican tropics. in the laboratory study, ma34, ma14 and a mixture of ma34+ma14 strains of m. anisopliae were evaluated for their control of the adult and larval stages of r. microplus, using the adult and larval immersion test respectively. the reproductive efficiency index of engorged females was determined in t ... | 2010 | 20299149 |
| evaluation of medicinal plant extracts against blood-sucking parasites. | the present study was based on assessments of the antiparasitic activities to determine the efficacies of acetone, chloroform, ethyl acetate, hexane, and methanol dried leaf, flower, and seed extracts of cassia auriculata l., rhinacanthus nasutus kurz., solanum torvum swartz, terminalia chebula retz., and vitex negundo linn. were tested against larvae of cattle tick rhipicephalus (boophilus) microplus canestrini, 1887 (acari: ixodidae), adult of haemaphysalis bispinosa neumann, 1897 (acarina: ix ... | 2010 | 20306205 |
| identification of a tsetse fly salivary protein with dual inhibitory action on human platelet aggregation. | tsetse flies (glossina sp.), the african trypanosome vectors, rely on anti-hemostatic compounds for efficient blood feeding. despite their medical importance, very few salivary proteins have been characterized and functionally annotated. | 2010 | 20351782 |
| virulence of mexican isolates of entomopathogenic fungi (hypocreales: clavicipitaceae) upon rhipicephalus=boophilus microplus (acari: ixodidae) larvae and the efficacy of conidia formulations to reduce larval tick density under field conditions. | the first objective was laboratory evaluation of the virulence of 53 mexican isolates of fungi against larvae of rhipicephalus (boophilus) microplus. thirty-three isolates of metarhizium anisopliae var. anisopliae (metschnickoff) sorokin (hypocreales: clavicipitaceae) and 20 isolates of isaria (paecilomyces) fumosorosea (fumosoroseus) (wize) (eurotiales: trichomaceae) were tested on 7-day-old larvae under laboratory conditions. larvae were immersed in a suspension containing 10(8)conidia/ml and ... | 2010 | 20359827 |
| novel technique for quantifying adhesion of metarhizium anisopliae conidia to the tick cuticle. | the present study describes an accurate quantitative method for quantifying the adherence of conidia to the arthropod cuticle and the dynamics of conidial germination on the host. the method was developed using conidia of metarhizium anisopliae var. anisopliae (metschn.) sorokin (hypocreales: clavicipitaceae) and engorged rhipicephalus annulatus (say) (arachnida: ixodidae) females and was also verified for m. anisopliae var. acridum driver et milner (hypocreales: clavicipitaceae) and alphitobius ... | 2010 | 20363785 |
| [action of extract and oil neem in the control of rhipicephalus (boophilus) microplus (canestrini, 1887) (acari: ixodidae) in laboratory]. | organic plant extracts and emulsified oil of azadirachta indica a. juss (meliaceae) (neem) were studied to evaluate its effects in control of engorged females of rhipicephalus (boophilus) microplus (canestrini, 1887) in the laboratory. hexane and alcoholic organic extracts, 2% (weight/volume) were used in tests of immersion for 5 minutes, prepared with seeds, solubilized in dimethylsulfoxide (dmso) to 1%. the experiment was entirely randomized, consisting of 6 treatments and 5 replicates, each r ... | 2010 | 20385059 |
| cold storage and cryopreservation of tick cell lines. | abstract: | 2010 | 20388200 |
| acaricidal activity and chemical composition of the essential oil from three piper species. | the chemical composition of the essential oil obtained by hydrodistillation of the aerial parts of piper amalago, piper mikanianum, and piper xylosteoides was elucidated by gas chromatography (gc) and gc/mass spectrometry analyses. p. mikanianum and p. xylosteoides essential oils presented phenylpropanoids as their main compounds (67.89% and 48.53%, respectively) whereas p. amalago was rich in monoterpene and sesquiterpene hydrocarbons (84.95%). the essential oils obtained were investigated for ... | 2010 | 20428889 |
| evidence for role of white-tailed deer (artiodactyla: cervidae) in epizootiology of cattle ticks and southern cattle ticks (acari: ixodidae) in reinfestations along the texas/mexico border in south texas: a review and update. | from 1907 when the fever tick eradication campaign began until 1933, the tick eradication methods of dipping cattle in an acaricide or "pasture vacation" were enormously successful in eradicating southern cattle ticks [rhipicephalus (boophilus) microplus (canestrini)], until failures began to occur in some areas of florida. regarding the failures in florida, the consensus was that populations of white-tailed deer [odocoileus virginianus (zimmermann)] infested with southern cattle ticks were resp ... | 2010 | 20429430 |
| genome wide scan for quantitative trait loci affecting tick resistance in cattle (bos taurus x bos indicus). | in tropical countries, losses caused by bovine tick rhipicephalus (boophilus) microplus infestation have a tremendous economic impact on cattle production systems. genetic variation between bos taurus and bos indicus to tick resistance and molecular biology tools might allow for the identification of molecular markers linked to resistance traits that could be used as an auxiliary tool in selection programs. the objective of this work was to identify qtl associated with tick resistance/susceptibi ... | 2010 | 20433753 |
| baculovirus expression, biochemical characterization and organophosphate sensitivity of rbmache1, rbmache2, and rbmache3 of rhipicephalus (boophilus) microplus. | rhipicephalus (boophilus) microplus cdnas, bmache1, bmache2, and bmache3, were previously identified as presumptively encoding acetylcholinesterases (aches), but biochemical identity was confirmed only for recombinant bmache3. in the present study, four recombinant bmache1 constructs and single recombinant constructs of bmache2 and bmache3 were expressed in baculovirus. biochemical characterization of the recombinant proteins supports classification of rbmache1, rbmache2, and rbmache3 as aches ( ... | 2010 | 20451328 |
| recombinant peptides as new immunogens for the control of the bovine tick, rhipicephalus (boophilus) microplus. | a candidate vaccine that effectively controls rhipicephalus microplus ticks in livestock is an attractive strategy in bovine management because of the expensive and labor-intensive treatment of animals with acaricides. however, the complex nature of ectoparasites has imposed restrictions on the development of such a vaccine, and its future efficacy is still under debate. using phage display technology, we developed specific immunogens that mimic r. microplus tick antigens and were successfully v ... | 2010 | 20488622 |