Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| effect of tyb-2285 on passive cutaneous anaphylaxis in rats. | 1. tyb-2285 (1-30 mg/kg p.o.) inhibited ovalbumin (oa)- and dinitrophenyl-ascaris (dnpas)-induced passive cutaneous anaphylaxis (pca) in a dose-dependent manner. 2. the ed50 of tyb-2285 and ketotifen fumarate on oa-induced pca were 0.5 and 3.9 mg/kg, respectively. the ed50 of typ-2285 and amlexanox on dnp-as-induced pca were 3.5 and 0.9 mg/ kg, respectively. 3. tyb-2285 (3-30 mg/kg p.o.) inhibited histamine consumption at the pca site. 4. unlike cyproheptadine or amlexanox, tyb-2285 (30 mg/kg p. ... | 1997 | 9013210 |
| role of circulating blood components and thromboxane in anaphylactic vasoconstriction in isolated canine lungs. | we determined the roles of circulating blood components and chemical mediators in anaphylactic vasoconstriction and microvascular permeability during ascaris suum antigen-induced anaphylaxis in isolated canine lungs. either the right or left lower lobe served as the control lung, which was perfused with autologous blood, and the contralateral lobe from the same dog was examined for the effect of albumin krebs-henseleit solution (krebs) or of blockers of various vasoconstrictors with blood perfus ... | 1997 | 9375313 |
| determination of airway and tissue resistances after antigen and methacholine in nonhuman primates. | antigen challenge of ascaris suum-sensitive animals has been used as a model of asthma in humans. however, no reports have separated total respiratory resistance into airway (raw) and tissue (rti) components. we compared input impedance (zin) and transfer impedance (ztr) to determine raw and rti in anesthetized cynomolgus monkeys under control and bronchoconstricted conditions. zin data between 1 and 64 hz are frequency dependent during baseline conditions, and this frequency dependence shifts i ... | 1997 | 9375340 |
| secretion of an aminopeptidase during transition of third- to fourth-stage larvae of ascaris suum. | protease activity was identified in culture fluids collected during in vitro development of l3 to l4 larval stages of ascaris suum. fluorogenic peptide substrates with unblocked n-termini were specifically hydrolyzed indicating aminopeptidase activity; a terminal arginyl residue was preferred. culture fluids did not hydrolyze fluorogenic peptide substrates with blocked n-termini (endopeptidase substrates). the aminopeptidase activity was inhibited by 1,10-phenanthroline (metalloprotease inhibito ... | 1997 | 9379278 |
| tachykinins contribute to the acute airways response to allergen in sheep actively sensitized to ascaris suum. | tachykinins, found in sensory nerves, have effects in the airways which suggest that they may contribute to the pathogenesis of asthma. we aimed to find evidence for tachykinin involvement in the immediate airway response to allergen in a sheep model of experimental asthma. twenty-four sheep were actively sensitized to ascaris suum, then challenged with nebulized ascaris extract in a dose-response fashion. change in lung resistance (rl) in response to challenge was measured. responder sheep (tho ... | 1997 | 9400681 |
| a trichuris specific diagnostic antigen from culture fluids of trichuris suis adult worms. | trichuris suis is a nematode parasite of swine which can cause serious pathology in infected pigs, such as anorexia, diarrhea, anemia, and death in heavy infections, especially in young animals. infection with trichuris suis is difficult to diagnose because much of the pathology occurs when only larval stages are present, and because of sporadic egg production by adult female worms. we have isolated a 20 kda excretory/secretory (e/s) glycoprotein from culture fluids of adult worms by column chro ... | 1997 | 9066055 |
| effect of tyb-2285 on antigen-induced accumulation of eosinophils into the peritoneal cavity of rats sensitized with ascaris suum extract. | 1. the present study was carried out to investigate the effect of 3,5-bis-(acetoxyacetylamino)-4-chloro-benzonitrile (tyb-2285) on the accumulation of eosinophils in the peritoneal cavity of wistar rats sensitized with ascaris suum extract (asc). 2. rats were sensitized by ip injection of asc on day 0 and were challenged by ip injection of asc on day 7. antigen challenge caused a specific, delayed infiltration of eosinophils into the peritoneal cavity that was inhibited by po administration of t ... | 1997 | 9068982 |
| apeaspfirfamide, a novel fmrfamide-related decapeptide from caenorhabditis elegans: structure and myoactivity. | to date, 9 fmrfamide-related peptides (farps) have been identified in caenorhabditis elegans. eight of these peptides are encoded on the flp-1 gene. however, af2 (kheylrfamide) which was not co-encoded was the most abundant farp identified in ethanolic extracts. further radioimmunometrical screening of acidified ethanol extracts of c. elegans has revealed the presence of other novel farps, which are not encoded on the flp-1 gene. one of these peptides has been isolated by sequential rphplc and s ... | 1997 | 9070852 |
| efficacy of doramectin against intestinal nematodes and sarcoptic manage mites in naturally infected swine. | endectocidal efficacy of doramectin administered intramuscularly at a dosage of 300 micrograms/kg was evaluated in 464 pigs naturally infected with intestinal nematodes or mange mites on 14 commercial farms in japan. by doramectin treatment, fecal egg counts were reduced > 99% for ascaris suum, strongyloides ransomi, oesophagostomum dentatum, and trichuris suis; worm counts of t. suis and mite counts of sarcoptes scabiei reduced 90.1% on day 21 and 99.5% on day 28 following treatment, respective ... | 1997 | 9070986 |
| the role of trehalose in the physiology of nematodes. | the sugar trehalose, an alpha-1-linked non-reducing disaccharide of glucose, is important in the physiology of many micro-organisms as well as in some groups of metazoan organisms, including insects and nematodes. trehalose is a stress protectant in biological systems as it interacts with and directly protects lipid membranes and proteins from the damage caused by environmental stresses such as desiccation and freezing. trehalose is present in many nematode species where its concentration often ... | 1997 | 9088992 |
| secretion of a novel, developmentally regulated fatty acid-binding protein into the perivitelline fluid of the parasitic nematode, ascaris suum. | early development of the parasitic nematode, ascaris suum, occurs inside a highly resistant eggshell, and the developing larva is bathed in perivitelline fluid. two-dimensional gel analysis of perivitelline fluid from infective larvae reveals seven major proteins; a cdna encoding one of these, as-p18, has been cloned, sequenced, and protein expressed in escherichia coli. the predicted amino acid sequence of as-p18 exhibits similarities to the intracellular lipid-binding protein (ilbp) family inc ... | 1997 | 9092532 |
| the basis of ige responses to specific antigenic determinants in helminthiasis. | 1997 | 9103663 | |
| ascaris suum: a revision of its early migratory path and implications for human ascariasis. | during the course of carrying out studies on the role of intestinal immunity in blocking the migration of larval ascaris suum in the pig, it was discovered that the prevailing understanding of larval penetration sites was at variance with our observations. therefore, a detailed investigation of the migration of a. suum 1.2 larvae through the intestine was initiated. the results demonstrate that the 1.2 larvae invade almost exclusively the wall of the pig cecum and colon and not the small intesti ... | 1997 | 9105307 |
| improved method for the recovery of ascarus suum larvae from pig intestinal mucosa. | in the course of a comprehensive study on the population biology of ascaris suum, it became necessary to determine quantitatively the migration of the larvae (l2) through the pig intestine. because no satisfactory methods for recovering these larvae from the intestinal mucosa were apparently available in the literature, we undertook the development of such a procedure. direct baermannization of the intestine proved inadequate, so a series of studies was undertaken to find a method to solubilize ... | 1997 | 9105321 |
| acetyl-coa hydrolase activity and function in ascaris suum muscle mitochondria. | acyl-coa compounds are stable in adult ascaris suum mitochondrial preparations. however, when incubated in the presence of 5,5'-dithio-bis(2-nitrobenzoic acid) (dtnb), acetyl-coa or propionyl-coa are hydrolyzed to form free coenzyme a. this acetyl-coa hydrolase activity has been partially purified and found to be specific for the above coa derivatives. gel filtration indicates an apparent molecular weight of 232,000. the hydrolase activity has been purified free from acyl-coa transferase activit ... | 1997 | 9114498 |
| bone marrow progenitors in allergic airways diseases: studies in canine and human models. | in a canine model of ascaris suum-inducible bronchial hyperresponsiveness, we previously demonstrated that bone marrow-derived myeloid progenitors rise within 24 h of allergen inhalation; this effect is abolished by pretreatment with inhaled budesonide. we now report that this allergen-induced bone marrow response is observable in human asthmatics, and involves increases in both neutrophil-macrophage and eosinophil-basophil progenitors, within 6 h of allergen inhalation, as measured either by he ... | 1997 | 9130516 |
| isolation and structural analysis of three neutral glycosphingolipids from a mixed population of caenorhabditis elegans (nematoda:rhabditida). | the free-living nematode, caenorhabditis elegans, has been proposed and analyzed as a prototypic model for parasitic nematodes. in order to study whether there is a structural basis for the proposed analogy with respect to nematode glycoconjugates, we have analyzed caenorhabditis elegans glycosphingolipids. three, simple neutral glycosphingolipid components of the neutral glycolipid fraction were isolated by high-performance liquid chromatography. structural analysis was performed by methylation ... | 1997 | 9134433 |
| evolution of the at-rich mitochondrial dna of the root knot nematode, meloidogyne hapla. | mitochondrial dna of the root knot nematode meloidogyne hapla was investigated for intraspecific diversity and divergence from other parthenogenetic root knot nematodes. a 1,900-bp fragment containing coii, trnahis, 16s rrna, nd3 and cyt b genes has been cloned and sequenced from one individual and an 1,188-bp region within this region was sequenced from four other australian isolates. m. hapla mtdna is more than 80% at-rich, like other meloidogyne spp. nucleotide diversity within m. hapla is so ... | 1997 | 9000752 |
| the effects of the peptide kpnfirfamide (pf4) on the somatic muscle cells of the parasitic nematode ascaris suum. | 1. commonly used anthelmintic agents act on the muscle cells of parasitic nematodes to cause paralysis of the parasite and its expulsion from the host. 2. the motonervous system of nematodes contains neuropeptides, many of which are myoactive and elicit prolonged worm paralysis. here we describe the actions of a novel peptide, kpnfirfamide (lys-pro-asn-phe-ileu-arg-phe-amide; pf4), which mediates relaxation of the somatic muscle of the parasitic nematode ascaris suum. its mechanism of action is ... | 1997 | 9031739 |
| primary sequence of mitochondrial trna(arg) of a nematode ascaris suum: occurrence of unmodified adenosine at the first position of the anticodon. | mitochondrial trna(arg) from a nematode, ascaris suum, was purified and sequenced at the rna level. an unmodified adenosine was found to exist at the anticodon first position, suggesting that, contrary to the conventional wobble rule, the anticodon acg of the trna can translate all the cgn codons. | 1997 | 9048878 |
| case report: eosinophilic colitis with high antibody titre against ascaris suum. | eosinophilic gastroenteritis (ege) is an inflammatory disease characterized by eosinophilic infiltration of the gastrointestinal tract accompanied by varying abdominal symptoms and usually by peripheral blood eosinophilia. although the precise aetiology of ege remains to be determined, contribution of allergic process to certain allergens, such as foods, drugs and parasites, has been repeatedly proposed as the pathogenesis of the disease. here we report on a rare case of a woman who had extensiv ... | 1997 | 9142635 |
| structural analysis of neutral glycosphingolipids from ascaris suum adults (nematoda:ascaridida). | the neutral glycosphingolipid fraction from adults of the pig parasitic nematode, ascaris suum, was resolved into four components on thin-layer chromatography. the high-performance liquid chromatography-isolated components were structurally analysed by: methylation analysis; exoglycosidase cleavage; gas-liquid chromatography/mass spectrometry; liquid secondary-ion mass spectrometry; and, in particular, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. their chemical s ... | 1997 | 9147062 |
| a bradykinin antagonist inhibits both bradykinin- and the allergen-induced airway response in primates. | bradykinin is a mediator of bronchoconstriction and may play a role in the development of the asthmatic response after antigen challenge. our objective was to study the effectiveness of npc 17731 as a specific bradykinin beta 2-receptor antagonist and as an antagonist of the allergen-induced early phase of asthma. a primate model was used for all studies. intracutaneous end-point titrations were performed with bradykinin. a shift of the bradykinin end-point titer was seen when npc 17731 was inje ... | 1997 | 9154643 |
| elastase contributes to antigen-induced mucociliary dysfunction in ovine airways. | antigen-induced bronchoconstriction is associated with impairment of mucociliary clearance with a time course that is consistent with the initial influx of neutrophils into the airway. in this study we tested the hypothesis that elastase released from activated neutrophils contributes to the acute (0 to 6-hr) antigen-induced mucociliary dysfunction. tracheal mucous velocity ctmv), an index of mucociliary function, was measured with a roentgenographic technique before and serially after airway ch ... | 1997 | 9154852 |
| expression of ascaris suum malic enzyme in a mutant escherichia coli allows production of succinic acid from glucose. | the malic enzyme gene of ascaris suum, was cloned into the vector ptrc99a in two forms encoding alternative amino-termini. the resulting plasmids, pmea1 and pmea2, were introduced into escherichia coli nzn111, a strain that is unable to grow fermentatively because of inactivation of the genes encoding pyruvate dissimilation. induction of pmea1, which encodes the native animoterminus, gave better overexpression of malic enzyme, approx 12-fold compared to uninduced cells. under the appropriate cul ... | 1997 | 9170244 |
| the impact of diets varying in carbohydrates resistant to endogenous enzymes and lignin on populations of ascaris suum and oesophagostomum dentatum in pigs. | the impact of diets varying in type and level of carbohydrates resistant to endogenous enzymes and lignin on the establishment and location of ascaris suum and oesophagostomum dentatum was investigated experimentally. fifty worm-free pigs, from a specific pathogen-free farm were used. the animals were assigned randomly to 5 diets and infected with 600 infective a. suum eggs and 6000 infective larvae of o. dentatum per pig. the diets consisted of a traditional ground barley plus protein feed (die ... | 1997 | 9172427 |
| expression, purification, and characterization of the recombinant nad-malic enzyme from ascaris suum. | the cdna encoding the 65-kda subunit of malic enzyme from ascaris suum was cloned into the bacterial expression vector pkk223-3 and overproduced in escherichia coli. a protein with a subunit molecular mass of 65,000 was expressed at a level of up to 3% of the total soluble protein in jm109, as judged by sds-page. the enzyme was purified using column chromatography on phenyl-sepharose followed by orange-a agarose. the purification procedure resulted in a 32-fold purification with an overall yield ... | 1997 | 9179290 |
| structural and functional analysis of a glutathione s-transferase from ascaris suum. | a recombinant glutathione s-transferase (gst) (ec 2.5.1.18) from the parasitic nematode ascaris suum (asgst1) displays specific activity with a variety of model substrates and secondary products of lipid peroxidation. the asgst1 interacts with a range of model inhibitors, haematin-related compounds, bile acids and anthelminthics. the reported variations in biochemical activity correlate with structural differences observed by homology modelling. here, differences in the topography of the propose ... | 1997 | 9182731 |
| the effect of fasting on ascaris suum and oesophagostomum spp. in growing pigs. | experiments were conducted to study the possible influence of fasting on ascaris suum and oesophagostomum spp. in growing pigs. forty young crossbred pigs naturally infected with a. suum and oesophagostomum spp. were used. in one experiment 10 pigs were fasted and offered water ad libitum for 6 days, in another experiment for 10 days. subsequently, these pigs, together with 10 non-fasted control pigs per experiment were slaughtered, and worm numbers, worm location, sex, developmental stage and f ... | 1997 | 9184936 |
| muscarinic hyperresponsiveness of antigen-sensitized feline airway smooth muscle in vitro. | to determine the effect of in vivo antigen sensitization (ascaris suum) of cats on tracheal smooth muscle (tsm) and bronchial smooth muscle (bsm) muscarinic reactivity in vitro. | 1997 | 9185978 |
| quantitative analysis of caenorhabditis elegans sperm motility and how it is affected by mutants spe11 and unc54. | the sperm of caenorhabditis elegans translocate in a fashion similar to sperm of ascaris suum even though their pseudopods are longer, more plastic in shape, and form multiple expansions zones around their perimeter. mutants in spe-11 form primary spermatocytes with a defective perinuclear region, but the resulting spermatozoa can still crawl and fertilize eggs. however, the resultant zygotes die due to the absence of sperm-supplied spe-11. computer-assisted analysis of translocating spe-11 sper ... | 1997 | 9186007 |
| transmission dynamics of helminth parasites of pigs on continuous pasture: ascaris suum and trichuris suis. | in denmark, alternative outdoor production systems for pigs are becoming more frequent, and information on the transmission of ascaris suum and trichuris suis under continuously grazed pasture conditions is needed. a group of pigs was turned out on a pasture in may 1993 (year 1 of the study), inoculated with 200 eggs of a. suum and 1000 eggs of t. suis, and followed parasitologically. a non-experimentally infected group of pigs was similarly turned out on the same pasture the following year (yea ... | 1997 | 9193950 |
| endectocidal efficacies of doramectin in naturally parasitized pigs. | the studies reported here were conducted to investigate the effectiveness of doramectin, given intramuscularly at the rate of 300 micrograms kg-1 of bodyweight, in the treatment of naturally acquired porcine nematodosis and acariasis. twenty pigs demonstrated to be naturally infected with pulmonary and gastrointestinal nematodes were used in one control study, and 22 pigs demonstrated to be naturally parasitized with sarcoptes scabiei var. suis were used in a second study. in both studies, anima ... | 1997 | 9195716 |
| evaluation of an elisa for the routine diagnosis of dictyocaulus viviparus infections in cattle. | an enzyme-linked immunosorbent assay (elisa) that detects antibodies against dictyocaulus viviparus in experimentally and naturally infected cattle was evaluated for its sensitivity, specificity, the moment of seroconversion and persistence of the anti-d. viviparus response and precision. the first three parameters were compared with those of an indirect haemagglutination assay (iha). specificity and sensitivity of both assays were assessed in sera collected from calves experimentally infected w ... | 1997 | 9195719 |
| inhibition of antigen-induced acute bronchoconstriction, airway hyperresponsiveness, and mast cell degranulation by a nonanticoagulant heparin: comparison with a low molecular weight heparin. | inhaled heparin prevents antigen-induced bronchoconstriction and inhibits anti-ige-mediated mast-cell degranulation. we hypothesized that the antiallergic action of heparin may be dependent on molecular weight and related to its nonanticoagulant properties. therefore, in the present investigation we studied the effects of a nonanticoagulant fraction of heparin (la-heparin) on antigen-induced bronchoconstriction, airway hyperresponsiveness (ahr), and mast-cell degranulation, and compared its anti ... | 1997 | 9196085 |
| in vitro anthelmintic activity of root-tuber extract of flemingia vestita, an indigenous plant in shillong, india. | the in vitro activity of root-tuber-peel extract of flemingia vestita, an indigenous plant consumed by the natives in northeast india, was tested against helminth parasites. live parasites (nematode: ascaris suum from pigs, a. lumbricoides from humans, ascaridia galli and heterakis gallinarum from domestic fowl; cestode: raillietina echinobothrida from domestic fowl; trematode: paramphistomum sp. from cattle) were collected in 0.9 % physiological buffered saline (pbs) and maintained at 37 +/- 1 ... | 1997 | 9197399 |
| isolation and partial characterization of a fatty-acid-binding protein from ascaris suum reproductive tissue. | a 12-kda fatty-acid-binding protein was purified to homogeneity from ascaris suum reproductive tissue as confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. n-terminal amino-acid-sequence analysis of the protein revealed its identity with the aba-1 allergen protein isolated from a. suum pseudocoelomic fluid. fatty-acid binding by the protein from a. suum reproductive tissue was investigated using the lipidex 1000 assay, which revealed the presence of a single class of fatty-a ... | 1997 | 9197404 |
| evidence for increased hydroxylation of pyrrolidone amino acid residues in the cuticle of mature onchocerca volvulus. | to determine whether morphologic changes are accompanied by variations in the biochemical and antigenic properties of the cuticle of onchocerca volvulus during development, we isolated and compared the 2-mercaptoethanol soluble cuticular proteins and the insoluble cuticlin from the predominant life-cycle stages occurring in man. sds-page analysis, before and after digestion with collagenase from achromobacter iophagus, revealed that the polypeptide composition of the 2-mercaptoethanol-solubilise ... | 1997 | 9197461 |
| onchocerca volvulus: immunohistochemical and immunoelectron microscopical distribution of a polyamine oxidizing enzyme. | we studied the distribution of a polyamine oxidizing enzyme (pao) in onchocerca volvulus and other nematode parasites by immunohistochemistry and electron microscopy with immunogold technique using a polyclonal antiserum raised against purified pao from ascaris suum. in adult o. volvulus the protein was localized in the outer zone and the area of the basal labyrinth of the hypodermis and occasionally in the outer zone of the uterine epithelium. further, the fluid in the body cavity was strongly ... | 1997 | 9217703 |
| cytochromes in the respiratory chain of helminth mitochondria. | parasitic helminths exhibit greater diversity in energy metabolism than do the host animals and many have exploited unique respiratory chains as adaptations to their natural habitats. cytochromes are involved, not only in intracellular aerobic respiration found in free-living stages, but also in the reduction of relatively oxidized compounds such as fumarate during the adult stages of parasitic helminths. in addition, most helminths retain a significant capacity to produce energy via aerobic pat ... | 1997 | 9229245 |
| the time course of the specific antibody response by various elisas in pigs experimentally infected with toxoplasma gondii. | with the aim of developing routine serological tests for monitoring the toxoplasma infection status of danish swine herds, four elisas based on tachyzoite antigen were set up: (1) an indirect elisa for igg-antibody; (2) a blocking elisa for antibody to the membrane antigen, p30; (3) an indirect elisa for igm; (4) a reverse, antibody-catching igm-elisa. groups of pigs (number between 6 and 10) were inoculated with tachyzoites of the rh-strain, tissue cysts of two complete strains, or oocysts in t ... | 1997 | 9231984 |
| increase of eosinophilic cell population in bone marrow of rats by immunization with ascaris suum antigen. | immunization of rats with the antigen, ascaris suum extract, increased the number of peripheral eosinophils. analysis by western blot and reverse transcription-polymerase chain reaction revealed that the levels of major basic protein and its mrna in the bone marrow were also increased, suggesting that eosinophilic cell population in the bone marrow is increased by the immunization. these findings indicate that immunization with this antigen stimulates differentiation of progenitor cells to eosin ... | 1997 | 9246564 |
| a myoglobin mutant designed to mimic the oxygen-avid ascaris suum hemoglobin: elucidation of the distal hydrogen bonding network by solution nmr. | the solution 1h nmr structure of the active site and ligand dissociation rate for the cyanomet complex have been determined for a sperm whale myoglobin triple mutant leu29(b10)-->tyr, his64(e7)-->gln, thr67(e10)-->arg that mimics the distal residue configuration of the oxygen-avid hemoglobin from ascaris suum. a double mutant that retains leu29(b10) was similarly investigated. two-dimensional nmr analysis of the iron-induced dipolar shifts, together with the conserved proximal side structure for ... | 1997 | 9251819 |
| localization of cytochrome oxidase and the 2-methyl branched-chain enoyl coa reductase in muscle and hypodermis of ascaris suum larvae and adults. | rabbit lung-derived third-stage larvae (l3) of ascaris suum are aerobic and cyanide sensitive but also contain many enzymes specific to anaerobic pathways. to localize these enzymes, diaminobenzidine (dab) staining for cytochrome oxidase (cox) and immunogold labeling for 2-methylbutyryl enoyl coa reductase (ecr) were performed on sections of hypodermis and muscle of adults and larvae of a. suum and visualized by transmission electron microscopy. as predicted, adult hypodermal and muscle mitochon ... | 1997 | 9267425 |
| survival of model helminth eggs and larvae (ascaris suum, oesophagostomum sp.) in the ensilaging process. | ascaris suum nonembryonated eggs remained viable for the most part even after 42 days of ensilaging. at the end of the anaerobic fermentation, mean of damaged eggs was 15.2 +/- 4.02 (min. 11, max. 21), 32.9%. conversely, the viability of oesophagostomum sp. nonembryonated eggs and infective l3 larvae was reduced-eggs: mean number 23.6 +/- 3.64 (min. 20. max. 28) specimens (93.3%), l3 larvae: mean number 24.2 +/- 4.38 (min. 19, max. 28) specimens (96.7%), during the period of study (42 days). con ... | 1997 | 9269126 |
| a patch clamp study of a glutamatergic chloride channel on pharyngeal muscle of the nematode ascaris suum. | glutamatergic chloride channels on the pharyngeal muscle of ascaris suum could be activated with glutamate and ivermectin and reversibly blocked with picrotoxin using the patch clamp technique. no activation was observed with gaba, glycine and acetylcholine. most of the current was carried by the main subconductance state of 21 ps. two smaller subconductance states occurred rarely. open time histograms could be best fitted by two time constants of tau(o1) = 0.33 ms and tau(o2) = 9.8 ms present a ... | 1997 | 9272691 |
| lactoferrin, a potent tryptase inhibitor, abolishes late-phase airway responses in allergic sheep. | tryptase, a serine protease released exclusively from activated mast cells, has been implicated as a potential causative agent in asthma. enzymatically active tryptase is comprised of four subunits, and heparin stabilizes the associated tetramer. lactoferrin, a cationic protein released from activated neutrophils, binds tightly to heparin, therefore we investigated lactoferrin as an inhibitor of tryptase and found that it is both a potent (ki' is 24 nm) and selective inhibitor. size exclusion ch ... | 1997 | 9279212 |
| false-positive ascaris suum egg counts in pigs. | false-positive ascaris suum egg counts in pig faeces are frequently observed under both experimental and natural conditions. data from 12 experiments with a. suum infections in pigs were summarized and showed that the percentage of false-positive faecal samples ranged from 4 to 36%. false-positive egg count values varied greatly between pigs and experiments (range 20-1060 eggs per gram faeces). indoor experiments with pigs housed groupwise in pens generally produced more and higher false-positiv ... | 1997 | 9279587 |
| validation of the specificity of the modified agglutination test for toxoplasmosis in pigs. | serum samples from toxoplasma gondii-negative pigs infected with protozoa, helminths and viruses, and from fetal pigs were examined for cross reacting antibodies to t. gondii by the use of the modified agglutination test (mat). nonspecific reactions with t. gondii antigen were not found in 1:10 dilutions of sera from eight pigs experimentally infected with sarcocystis miescheriana, 11 pigs infected with ascaris suum, three pigs infected with trichuris suis, ten pigs infected with trichinella spi ... | 1997 | 9299699 |
| blockade of late-phase airway responses and airway hyperresponsiveness in allergic sheep with a small-molecule peptide inhibitor of vla-4. | the leukocyte integrin very late antigen-4 (vla-4) (alpha 4 beta 1, cd49d/cd29) is an adhesion receptor predominantly expressed on lymphocytes, monocytes, and eosinophils, but not on neutrophils. recent studies with monoclonal antibodies against vla-4 suggest that antigen-induced late responses and airway hyperresponsiveness (ahr) may depend on the recruitment and/or activation of vla-4-expressing leukocytes. to further test this hypothesis, we administered by aerosol either a potent small-molec ... | 1997 | 9309981 |
| cloning, structural analysis, and expression of the pig ige epsilon chain. | as a step in the evolutionary studies of immunoglobulin e (ige) and for the purpose of developing new reagents that will facilitate a more detailed analysis of ige-mediated inflammatory reactions in a large animal model, we here present the cloning of the epsilon chain of ige in the domestic pig (sus scrufa). a partial cdna clone for the epsilon chain of pig ige was isolated by polymerase chain reaction (pcr) amplification using degenerate primers directed against conserved regions in the second ... | 1997 | 9321425 |
| a comparison of functional and structural consequences of the tyrosine b10 and glutamine e7 motifs in two invertebrate hemoglobins (ascaris suum and lucina pectinata). | the architecture of the distal heme pocket in hemoglobins and myoglobins can play an important role in controlling ligand binding dynamics. the size and polarity of the residues occupying the distal pocket may contribute steric and dielectric effects. in vertebrate systems, the distal pocket typically contains a "distal" histidine at position e7 and a leucine at position b10. there are several invertebrate organisms that have hemoglobins or myoglobins that display a pattern in which residues e7 ... | 1997 | 9335574 |
| afp-1: a gene encoding multiple transcripts of a new class of fmrfamide-like neuropeptides in the nematode ascaris suum. | we have identified a gene, afp-1, that encodes a new subfamily of six fmrfamide-like neuropeptides in the nematode ascaris suum. the predicted peptides share the c-terminal sequence pgvlrf-nh2 but have different n-terminal extensions. we discuss possible functional roles of these different peptides based upon experiments with ascaris as well as results from other organisms. three of the peptides were previously isolated from extracts of a. suum (4) and three other are novel sequences. the transl ... | 1997 | 9357048 |
| helminthosis in local and cross-bred pigs in the morogoro region of tanzania. | we investigated the prevalence, burden and types of gastro-intestinal helminths in 424 local and cross-bred pigs kept under different management systems in two climatic zones in the morogoro region of tanzania. coprological examination revealed that 53% of the pigs excreted helminth eggs in their faeces. the median eggs per gram of faeces (epg) was 500 (range 100-22,000). local breeds in the mgeta location with tropical highland climate showed significantly higher prevalence (p < 0.001) and medi ... | 1997 | 9361319 |
| sex-manipulated ascaris suum infections in pigs: implications for reproduction. | in spite of the vast distribution and considerable impact on human and animal health of ascaris suum and a. lumbricoides, little is known of the sexual biology and reproductive capacity of these intestinal nematodes. by oral transfer of adult female worms to previously parasite-naive pigs we show that in the absence of males the egg production ceases after 2-3 weeks. such females readily resume egg production a few days after oral transfer of male worms. these observations throw light on an impo ... | 1997 | 9364571 |
| experimental ascaris suum infection in the pig: worm population kinetics following single inoculations with three doses of infective eggs. | to study population kinetics during primary ascaris suum infections, 3 groups of 52 pigs each were inoculated with 100, 1000, or 10,000 infective eggs. in all groups, the majority of larvae was found in the liver on day 3 post inoculation (p.i.) and in the lungs on day 7 p.i. liver white spots, caused by migrating larvae, were most numerous at day 7 p.i., whereafter they gradually healed, and only low numbers of granulation-tissue type white spots and lymphonodular white spots persisted at days ... | 1997 | 9364572 |
| purification and properties of a membrane aminopeptidase from ascaris suum muscle that degrades neuropeptides af1 and af2. | we have identified on the membranes of the locomotory muscle of ascaris suum an amastatin-sensitive aminopeptidase that hydrolyses the bioactive neuropeptides af1 (knefirf-nh2) and af2 (kheylrf-nh2), by cleavage of the lys1-asn2 and lys1-his2 peptide bonds, respectively. af2 (1.2 nmol of heylrf-nh2 formed min[-1] (mg protein[-1])) was hydrolysed at a faster rate compared to af1 (0.2 nmol of nefirf-nh2 formed min[-1] (mg protein[-1])). af1 hydrolysis by the aminopeptidase was inhibited by the ama ... | 1997 | 9364967 |
| actions of the anthelmintic ivermectin on the pharyngeal muscle of the parasitic nematode, ascaris suum. | the anthelmintic invermectin has a number of effects on nematodes which result in changes in behaviour, particularly locomotion, including paralysis and an inhibition of feeding. this paper describes the application of an in vitro pharmacological approach to further delineate the action of ivermectin on feeding behaviour. contraction of ascaris suum pharyngeal muscle was monitored using a modified pressure transducer system which detects changes in intrapharyngeal pressure and therefore contract ... | 1997 | 9368907 |
| serological assays for the identification of oesophagostomum dentatum infections in pigs. | oesophagostomum dentatum antigen preparations of third (l3) or fourth (l4) stage larvae were characterised by western blotting. panels of sera obtained from pigs infected with o dentatum and ascaris suum, respectively, reacted with the same bands of l3 antigen. in contrast high specificity against a characteristic band, was observed when l4 extract was employed as antigen. to establish an enzyme-linked immunosorbent assay (elisa), a panel of homologous and heterologous sera was tested against o ... | 1997 | 9368949 |
| [development of an igy-based assay for the detection of ascaris-suum-antigens] | the presented paper describes the development of a coproantigen-test for the detection of ascariosis in pigs using vitelline chicken antibodies. the method used for these investigations was an indirect two-sided-enzyme-immuno-assay (eia), which was set up as follows: affinity-purified rabbit antibody was bound to the microtiter-plate as the catching antibody. after the faeces extract to be tested followed the primary antibody, which was a roughly purified avian antibody (igy). this again could b ... | 1996 | 11178475 |
| microevolutionary patterns and molecular markers: the genetics of geographic variation in ascaris suum. | molecular markers have been used only rarely to characterize the population genetic structure of nematodes. published studies have suggested that different taxa may show distinct genetic architectures. isoenzyme and rapd markers have been used to investigate geographic variation of ascaris suum at the level of infrapopulations (nematodes within individual hosts), within localities, and among geographic regions. independent estimates of genetic differentiation among population samples based on is ... | 1996 | 19277145 |
| the motornervous system of ascaris: electrophysiology and anatomy of the neurons and their control by neuromodulators. | analysis of the electrical properties of neurons in the motornervous system of ascaris sutom suggests that it is largely an analogue system. the motorneurons do not conduct action potentials and they release transmitter tonically at their normal resting potential; transmitter release is increased or decreased as a continuous function of membrane potential. despite extensive physiological descriptions of the electrical properties of the neurons and their synapses, as well as morphological descrip ... | 1996 | 9051930 |
| fmrfamide-related peptides (farps) in nematodes: occurrence and neuromuscular physiology. | the occurrence of classical neurotransmitter molecules and numerous peptidic messenger molecules in nematode nervous systems indicate that although structurally simple, nematode nervous systems are chemically complex. thus far, studies on one nematode neuropeptide family, namely the fmrfamide-related peptides (farps), have revealed an unexpected variety of neuropeptide structures in both free-living and parasitic species. to date 23 nematode farps have been structurally characterized including 1 ... | 1996 | 9051931 |
| electrophysiology of ascaris muscle and anti-nematodal drug action. | three groups of anthelmintic drugs act directly and selectively on muscle membrane receptors of parasitic nematodes. these groups of anthelmintics are: (1) the nicotinic agonists (levamisole, pyrantel, morantel and oxantel) that act on acetylcholine receptors of nematode somatic muscle; (2) the gaba agonist, piperazine, that acts on nematode muscle gaba receptors; and (3) the avermectins that open glutamate gated cl- channels on nematode pharyngeal muscle. the electrophysiology and pharmacology ... | 1996 | 9051932 |
| automated chemical synthesis of biologically active trna having a sequence corresponding to ascaris suum mitochondrial trna(met) toward nmr measurements. | rna samples corresponding to ascaris suum mitochondrial trna(met) were chemically and automatically synthesized in amounts sufficient for nmr measurement. conventional and rapid deprotection methods gave trna samples with the same amino acid-accepting activity as those prepared by other method; enzymatic synthesis, and enzymatic ligation of chemically synthesized fragments. the synthetic trna showed the same 1h-nmr spectrum in the iminoproton region as the ligated trna. this rapid and reliable p ... | 1996 | 9010751 |
| [the effect of cydectin on the survival of second stage larvae and mature ascaris suum cultured in vitro]. | larvae of ascaris suum (l2) newly hatched from eggs with sodium hypochlorite, were placed (2000 larvae per 2 ml) of culture in eagle's medium, containing 10% calve serum or without it. moxidectin (cydectin) was introduced into medium in concentrations: 5, 10, 25 and 50 micrograms/ml. the cultures were incubated for 3 days, and there were controlled every 12 hours. the effect of drug on survival of larvae was slightly expressed. after 3 days survival rates were 87, 81, 80 and 78% in medium with s ... | 1996 | 9012134 |
| an electrophysiological preparation of ascaris suum pharyngeal muscle reveals a glutamate-gated chloride channel sensitive to the avermectin analogue, milbemycin d. | an electrophysiological preparation of ascaris suum pharyngeal muscle suitable for recording changes of input conductance using a 2-microelectrode current clamp and pharmacological study is described. the preparation is shown to contain a glutamate-gated cl (ion sensitive) channel sensitive to the avermectin analogue, milbemycin d. the application of glutamate produces a dose-dependent increase in cl conductance and the effect of glutamate is potentiated by milbemycin d. milbemycin d also produc ... | 1996 | 8851865 |
| novel azole derivatives are antagonists at the inhibitory gaba receptor on the somatic muscle cells of the parasitic nematode ascaris suum. | the somatic muscle cells of the parasitic nematode ascaris suum possess gaba receptors that gate chloride conductances in a similar fashion to the mammalian gabaa receptor subtype. these receptors mediate muscle relaxation and are the site of action of the anthelmintic piperazine. the properties of this receptor differ from the properties of the gaba-gated chloride receptors in the mammalian host, in particular they are not as sensitive to mammalian gaba receptor antagonists such as bicuculline ... | 1996 | 8851866 |
| can ascaris suum cause visceral larva migrans? | 1996 | 8782781 | |
| purification and characterization of gamma-glutamyl transpeptidase from ascaris suum. | gamma-glutamyl transpeptidase, which is of central importance in the degradation of glutathione, was purified from ascaris suum to apparent homogeneity. the enzyme was found to be a predominantly membrane-bound protein and was solubilized by triton x-100. the purified enzyme, which exhibits a specific activity of 1009 u (mg protein)-1, showed a molecular mass of 70 kda and was found to be composed of two non-identical subunits of molecular mass 43 and 30 kda. concerning the kinetic properties of ... | 1996 | 8784770 |
| enhanced immunity to hyalomma anatolicum anatolicum ticks in cross-bred (bos indicus x bos taurus) calves using ascaris extract immunomodulator with the tick salivary gland extract antigens. | development of acquired immunity in cross-bred (bos indicus x bos taurus) calves against hyalomma anatolicum anatolicum, a tick vector of bovine tropical theileriosis was studied using ascaris extract (ae), an immunomodulator of ige responses, along with the tick salivary gland extract (sge) antigens in freund's incomplete adjuvant (fia) emulsion. calves immunised with sge + fia showed significant rejection (47.4 +/- 2.8%) of larvae, whereas only marginal rejection (12.47 +/- 1.5%) of nymphs was ... | 1996 | 8792570 |
| a possible influence of diet composition on the establishment of nematodes in the pig. | two groups of 20 pigs weighing approx. 26 kg were offered either ground barley plus protein supplement (gbp) or commercial pelleted feed (cpf), two diets consisting of nearly the same amounts of carbohydrates, proteins and micronutrients. all pigs were experimentally infected with 600 infective ascaris suum eggs and 6000 infective oesophagostomum spp. larvae and followed coprologically for approx. 12 weeks post infection, whereafter they were slaughtered. the number of oesophagostomum spp. and a ... | 1996 | 8792589 |
| effects of local and systemic budesonide on allergen-induced airway reactions in the pig. | 1. in this study, an attempt was made to distinguish between local and systemic effects of low doses of the topical glucocorticoid, budesonide. the effect of aerosolized budesonide administered to the lower airways versus intravenously administered budesonide on the acute and late response to nebulized ascaris suum extract in the lung, was evaluated in the minipig after active sensitization with purified a. suum antigen. budesonide was administered once, 1 h prior to a. suum challenge and airway ... | 1996 | 8799573 |
| heterogeneity of cholecystokinin/gastrin-like immunoreactivity in the nervous system of the nematode ascaris suum. | a wholemount immunocytochemical method was used for the localization of cholecystokinin (cck8)-like and gastrin-like immunoreactivity in ascaris. the patterns of specific neuronal staining given by two antisera and four monoclonal antibodies made against cck8, and one antiserum made against gastrin were investigated. preabsorption of these antibodies with cck8 or gastrin 17 resulted in complete loss of immunoreactivity in almost all of the neurons (two antisera also contained nonspecific antibod ... | 1996 | 8807446 |
| effect of tyb-2285 on antigen-induced airway responses in sheep. | we studied the effect of a new antiallergic compound, tyb-2285 (3,5-bis(acetoxyacetylamino)-4-chlorobenzonitrile), on antigen-induced early and late bronchoconstriction and airway responsiveness in conscious allergic sheep. the same general protocol was used for all studies, except that the dosage and time of tyb-2285 treatment was varied. the basic protocol consisted of determining airway responsiveness to inhaled carbachol, then measuring the airway responses to ascaris suum antigen challenge ... | 1996 | 8843510 |
| molecular and functional properties of cytochrome c from adult ascaris suum muscle. | mitochondrial cytochrome c was isolated at high purity from adult ascaris suum muscle and its molecular properties were investigated. the molecular weight of a. suum cytochrome c was determined to be 13,119 by electrospray ionization mass spectrometry. the oxidation-reduction potential of nematode cytochrome c was measured to be +248 mv; this value is comparable to those for cytochrome c from mammalian sources. the a. suum cytochrome c, like bovine heart cytochrome c, showed biphasic kinetics ag ... | 1996 | 8844672 |
| role of the divalent metal ion in the nad:malic enzyme reaction: an eseem determination of the ground state conformation of malate in the e:mn:malate complex. | the conformation of l-malate bound at the active site of ascaris suum malic enzyme has been investigated by electron spin echo envelope modulation spectroscopy. dipolar interactions between mn2+ bound to the enzyme active site and deuterium specifically placed at the 2-position, the 3r-position, and the 3s-position of l-malate were observed. the intensities of these interactions are related to the distance between each deuterium and mn2+. several models of possible mn-malate complexes were const ... | 1996 | 8844853 |
| immunisation regimes used to elevate serum ige in sheep. | six different immunisation regimes were used in an attempt to elevate the concentration of ovine ige. the response was measured by a passive cutaneous anaphylaxis (pca) test. these regimes included infection with ascaris suum and the combination of infection and parenteral administration of ascaris antigens and ovalbumin in adjuvants. the regime producing the highest titre of ige was the combination of ascaris infection with parenteral administration of ascaris extract in bordetella pertussis. t ... | 1996 | 9014313 |
| granulocyte function in the airways of allergen-challenged pigs: effects of inhaled and systemic budesonide. | late airways obstruction and eosinophil infiltration after allergen challenge are often seen in human asthma and animal models of allergy. this inflammatory reaction, which may be a link between acute and chronic asthma, is blocked by glucocorticoid pretreatment. however, the role of eosinophils in late airways obstruction and the primary site of action of glucocorticoids, i.e. locally or systemically, have not been fully determined. | 1996 | 9027445 |
| a newly formed telomere in ascaris suum does not exert a telomere position effect on a nearby gene. | during the process of chromatin diminution in ascaris suum (formerly named ascaris lumbricoides var. suum), developmentally regulated chromosomal fragmentation and new telomere addition occur within specific chromosomal breakage regions (cbrs). the dna sequences flanking one of these cbrs (cbr-1) were analyzed, and two protein-encoding genes were found on either side. the noneliminated gene, agp-1, whose aug start codon is located within approximately 2 kb of the boundary of cbr-1, encodes a put ... | 1996 | 8524289 |
| hydrogen bonding of tyrosine b10 to heme-bound oxygen in ascaris hemoglobin. direct evidence from uv resonance raman spectroscopy. | the hemoglobin from ascaris suum, a parasitic nematode, has a spontaneous dissociation rate for the dioxygen ligand that is 3 orders of magnitude less than for mammalian myoglobins or hemoglobins. in this hemoglobin, the distal histidine is replaced with a glutamine which is capable of forming a stabilizing hydrogen bond to the bound dioxygen. a single hydrogen bond from a glutamine is, under typical circumstances, not sufficient to account for the low off rate for oxygen. several studies point ... | 1996 | 8557711 |
| in vitro and in vivo effects of leukotriene b4 antagonism in a primate model of asthma. | to test the hypothesis that leukotriene (lt) b4 antagonists may be clinically useful in the treatment of asthma, cp-105,696 was evaluated in vitro, using chemotaxis and flow cytometry assays, and in vivo, using a primate asthma model. cp-105,696 inhibited ltb4-mediated monkey neutrophil chemotaxis (isolated cells, ltb4 = 5 nm) and cd11b upregulation (whole blood, ltb4 = 100 nm) with ic50 values of 20 nm and 16.5 microm, respectively. using a modification of a previously described in vivo protoco ... | 1996 | 8567958 |
| nadph diaphorase activity in peptidergic neurones of the parasitic nematode, ascaris suum. | the histochemical marker for nitric oxide synthase, nadph diaphorase, is known to co-localize in mammalian neurones with various classical neurotransmitters and neuropeptides. the nervous system of the parasitic nematode ascaris suum has previously been shown to contain both nadph diaphorase activity and neuropeptide immunoreactivity. this study examined the possibility that nadph diaphorase and neuropeptide immunoreactivity may co-exist in the same neurones. two antisera were used, one raised t ... | 1996 | 8587795 |
| preparation of biologically active ascaris suum mitochondrial trnamet with a tv-replacement loop by ligation of chemically synthesized rna fragments. | ascaris suum mitochondrial trna met lacking the entire t stem was prepared by enzymatic ligation of two chemically synthesized rna fragments. the synthetic trna could be charged with methionine by a.suum mitochondrial extract, although the charging activity was considerably low compared with that of the native trna, probably due to lack of modification. enzymatic probing of the synthetic trna showed a very similar digestion pattern to that of the native trna met, which has already been concluded ... | 1996 | 8604307 |
| isotope partitioning with ascaris suum phosphofructokinase is consistent with an ordered kinetic mechanism. | isotope partitioning and initial velocity studies have been used to study the kinetic mechanism of ascaris suum phosphofructokinase (pfk) at ph 8.0 for the native enzyme (npfk), and at ph 6.8 for a form of enzyme desensitized (dpfk) to hysteresis in the reaction time course, to atp allosteric inhibition, and to f6p homotropic cooperativity. complete trapping (p*max approximately equal to 100%) of the e:mgatp* complex as fructose (1-32p)-1, 6-bisophosphate for both enzyme forms is consistent with ... | 1996 | 8611535 |
| behavioral and cellular effects of serotonin on locomotion and male mating posture in ascaris suum (nematoda). | the site and mode of action of serotonin on locomotion were investigated in the parasitic nematode ascaris suum. injection of serotonin into ascaris immediately caused paralysis in animals that were generating locomotory waveforms. injected serotonin also increased body length and decreased the number of propagating body waves. similar injections into the male tail produced a ventral tail curl. injection of n-acetyl-serotonin had no effect on the generation of locomotory waveforms, but increased ... | 1996 | 8618217 |
| [the effect of copper on immune responses in guinea pigs with experimental ascariasis]. | a subchronic effect of copper on selected immunological parameters was studied in guinea pigs with experimental ascariosis (ascaris suum). cuso4 given orally for 28 days moderately increased t and b cells in the animal lymphoid organs. ch50 and ah50 complement levels remained unchanged during cuso4 administration. a subsequent infection of subchronically intoxicated guinea pigs caused a repeated insignificant stimulation of b cell values while the percentage of t cells was not increased. on the ... | 1996 | 8619277 |
| identification of a novel dihydrolipoyl dehydrogenase-binding protein in the pyruvate dehydrogenase complex of the anaerobic parasitic nematode, ascaris suum. | a novel dihydrolipoyl dehydrogenase-binding protein (e3bp) which lacks an amino-terminal lipoyl domain, p45, has been identified in the pyruvate dehydrogenase complex (pdc) of the adult parasitic nematode, ascaris suum. sequence at the amino terminus of p45 exhibited significant similarity with internal e3-binding domains of dihydrolipoyl transacetylase (e2) and e3bp. dissociation and resolution of a pyruvate dehydrogenase-depleted adult a. suum pdc in guanidine hydrochloride resulted in two e3- ... | 1996 | 8621401 |
| purification and molecular characterization of a novel b5-type cytochrome of the parasitic nematode, ascaris suum. | a b5-type cytochrome was extracted from ascaris suum muscle at ph 4.5 with 0.3% aluminum sulfate and purified by ammonium sulfate fractionation, ion-exchange chromatography on cm-500 cellulofine, and gel filtration on sephadex g-75. the hemoprotein displayed a typical absorption spectrum of cytochrome b with a midpoint redox potential of 78 mv. the n-terminal amino acid sequence was determined and revealed the n-terminus to be highly homologous to the heme-binding domain of vertebrate cytochrome ... | 1996 | 8638926 |
| protective effect of heparin on immunologically induced tracheal smooth muscle contraction in vitro. | our previous studies have shown that heparin, a competitive inhibitor of inositol triphosphate receptors, inhibits airway anaphylaxis in vivo. in the present study, we tested the hypothesis that heparin blocks immunologically induced tracheal smooth muscle (tsm) contraction in vitro. tsm was obtained from sheep allergic to ascaris suum antigen, and was suspended in an organ bath containing oxygenated (95% o2, 5% co2) krebs-henseleit buffer at 39 degrees c. after an equilibration period, the tiss ... | 1996 | 8645983 |
| isolation and characterization of porcine cationic eosinophil granule proteins. | the allergic pig can be used as a large-animal model for studies of allergic reactions in the airways and the role of eosinophils in such reactions. to measure the activation of eosinophils, the release of eosinophil-derived cationic proteins can be used. the purpose of this study was to isolate and characterize cationic proteins derived from porcine eosinophils. pigs were infested with live ascaris suum eggs to induce eosinophilia (greater than or = 40% of leukocytes). blood was collected and l ... | 1996 | 8645990 |
| an outbreak of visceral larva migrans due to ascaris suum in kyushu, japan. | 1996 | 8656926 | |
| comparison of the substrate specificities of camp-dependent protein kinase from bovine heart and ascaris suum muscle. | the catalytic subunits of camp-dependent protein kinases (protein kinase a) from bovine heart and ascaris suum muscle exhibit only 48% sequence identity and show quantitative differences in substrate specificity. these differences were not obvious at the level of short synthetic substrate peptides but were distinct for some protein substrates. phosphofructokinase from ascaris, a physiological substrate, was a better substrate for the protein kinase from the nematode in comparison to the mammalia ... | 1996 | 8722322 |
| influence of endogenous cortisol on eosinophil function in sensitized pigs: direct measurements of eosinophil peroxidase. | eosinophils are associated with bronchial asthma, but the role of the eosinophil is not fully understood. this study was initiated in order to study the influence of endogenous cortisol on eosinophil recruitment and activation in allergic inflammation in the lower airways in the pig. | 1996 | 8732245 |
| structure-activity relationships of knefirfamide (af1), a nematode fmrfamide-related peptide, on ascaris suum muscle. | analogues of knefirfamide (lys-asn-glu-phe-ile-arg-phe-nh2; af1), an fmrfamide-related peptide (farp) originally isolated from ascaris suum, were characterized in an a. suum muscle tension assay. af1 had biphasic effects on this preparation, inducing a brief relaxation followed by excitation and spastic paralysis. activity of af1 in this assay was eliminated by n-terminal deletions and by deamidation of the carboxy-terminus. the potency of af1 was greatly reduced by alanine substitution for any ... | 1996 | 8735963 |
| inhibition of glutathione synthesis of ascaris suum by buthionine sulfoximine. | we investigated the effect of dl-buthionine-s,r-sulfoximine (bso), a selective glutathione (gsh)-depleting agent, on the gsh synthesis of ascaris suum. the gsh concentrations of the reproductive and muscle tissues of a. suum were determined to be 8.5 +/- 0.3 and 14.3 +/- 1.3 (n = 3) nmol/mg protein, respectively. after treatment of the parasites with 10 microm bso for 24 h, the gsh content of the reproductive tissue of a. suum was totally depleted as compared with that of untreated controls. how ... | 1996 | 8740556 |
| antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and other nematodes. | we used elisa and immunoblotting to investigate antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and five other nematodes: the ascaridoids ascaris suum, toxocara canis and hysterothylacium aduncum, and the nonascaridoids trichinella spiralis and trichuris muris. two sera were raised against each species (including a. simplex, but excluding a. suum), by infection or by immunization with somatic antigens. serum against a. suum was raised by immunization only. the r ... | 1996 | 8740558 |
| bsti, a trypsin inhibitor from skin secretions of bombina bombina related to protease inhibitors of nematodes. | from skin secretions of the european frog bombina bombina, a new peptide has been isolated that contains 60 amino acids, including 10 cysteine residues. its sequence was determined by automated edman degradation and confirmed by analysis of the cdna encoding the precursor. a search in the databanks demonstrated that the pattern of cysteine residues in this skin peptide is similar to the ones found in protease inhibitors from ascaris and in a segment of human von willebrand factor. the 3d structu ... | 1996 | 8745414 |
| study on a new egg count technique for macracanthorhynchus hirudinaceus and ascaris suum. | this paper deals with a new egg count technique for macracanthorhynchus hirudinaceus and ascaris suum. the making of the new counter and the operation are described. the eggs of m. hirudinaceus in the upper, the middle and the lower levels of the sediment from swine feces were counted by the new egg count technique and the numbers of the eggs in the sedimentary liquids diluted two, four, six, eight and ten times were nearly similar. this indicates that the eggs dispersed evenly in the sediment a ... | 1996 | 8750688 |
| non-t cell-derived il-4 plays an important role in ige production induced by antigen resensitization and is resistant to fk506. | the present study investigates the role of non-t cell-derived il-4 in ige production. it is well known that il-4 is generally produced by t cells and induces ige production. we have demonstrated that non-t cells also produce il-4 in vivo by injection with ag following passive sensitization of mice with ige. in contrast to its known potent immunosuppressive effects on t cells, fk506 only partially inhibited il-4 produced by non-t cells; therefore, fk506 was found to be a useful tool for identifyi ... | 1996 | 8752921 |