Publications

dissecting the genome of the polyploid crop oilseed rape by transcriptome sequencing.	polyploidy complicates genomics-based breeding of many crops, including wheat, potato, cotton, oat and sugarcane. to address this challenge, we sequenced leaf transcriptomes across a mapping population of the polyploid crop oilseed rape (brassica napus) and representative ancestors of the parents of the population. analysis of sequence variation and transcript abundance enabled us to construct twin single nucleotide polymorphism linkage maps of b. napus, comprising 23,037 markers. we used these ...	2011	21804563
expression analysis of sugarcane shaggy-like kinase (susk) gene identified through cdna subtractive hybridization in sugarcane (saccharum officinarum l.).	identification of genes whose expression enables plants to adapt to any kind of stresses is integral to developing stress tolerance in crop plants. in this study, pcr-based cdna suppression subtractive hybridization technique was used to construct sugarcane salt (nacl) stress specific forward and reverse subtracted cdna library. for this, mrnas were pooled from the shoot and root tissues stressed with nacl (200 mm) for various time intervals (0.5 to 18 h). sequencing the clones from the forward ...	2011	20853012
use of sugarcane molasses "b" as an alternative for ethanol production with wild-type yeast saccharomyces cerevisiae itv-01 at high sugar concentrations.	molasses "b" is a rich co-product of the sugarcane process. it is obtained from the second step of crystallization and is richer in fermentable sugars (50-65%) than the final molasses, with a lower non-sugar solid content (18-33%); this co-product also contains good vitamin and mineral levels. the use of molasses "b" for ethanol production could be a good option for the sugarcane industry when cane sugar prices diminish in the market. in a complex medium like molasses, osmotolerance is a desirab ...	2011	21971607
Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil.	Sugarcane is an important agricultural product of Brazil, with a total production of more than 500 million tons. Knowledge of the bacterial community associated with agricultural crops and the soil status is a decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria associated with the plant by culture-dependent approaches. Culture-independent approaches allow a more comprehensive view of entire ba ...	2011	22042267
Developing a sustainable phytomanagement strategy for excessive selenium in western United States and India.	Phytomanagement technology is recognized as an inexpensive and environmental friendly strategy for managing natural-occurring selenium (Se) in soils and in poor quality waters. Multi-year field and greenhouse studies were conducted with different plant species in California, USA and Punjab, India under high Se growing conditions. Some of the plant species included; canola (Brassica napus), mustard (B. juncea), broccoli (B. oleracea), spearmint (Mentha viridis), sugarcane (Saccharum officcinarum) ...	2011	22046761
Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene codes for 130-kDa toxin protein.	Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene from Bacillus thuringiensis var. kurstaki borne on pKT230, shuttle vector, was generated. PCR amplification of Cry1Ac gene present in recombinant G. diazotrophicus yielded a 278-bp DNA product. The nitrogenase assay has revealed that the recombinant G. diazotrophicus in sugarcane stem produced similar levels of nitrogenase compared to wild-type G. diazotrophicus. The presence of 130-kDa protein in apoplastic fluid from sugarcane ...	2011	21934327
down regulation of a gene for cadherin, but not alkaline phosphatase, associated with cry1ab resistance in the sugarcane borer diatraea saccharalis.	the sugarcane borer, diatraea saccharalis, is a major target pest of transgenic corn expressing bacillus thuringiensis (bt) proteins (i.e., cry1ab) in south america and the mid-southern region of the united states. evolution of insecticide resistance in such target pests is a major threat to the durability of transgenic bt crops. understanding the pests' resistance mechanisms will facilitate development of effective strategies for delaying or countering resistance. alterations in expression of c ...	2011	21991350
Enzymatic hydrolysis and simultaneous saccharification and fermentation of alkali/peracetic acid-pretreated sugarcane bagasse for ethanol and 2,3-butanediol production.	The enzymatic digestibility of alkali/peracetic acid (PAA)-pretreated bagasse was systematically investigated. The effects of initial solid consistency, cellulase loading and addition of supplemental ß-glucosidase on the enzymatic conversion of glycan were studied. It was found the alkali-PAA pulp showed excellent enzymatic digestibility. The enzymatic glycan conversion could reach about 80% after 24 h incubation when enzyme loading was 10 FPU/g solid. Simultaneous saccharification and fermentat ...	2011	22112569
molecular characterization of a new strain of sugarcane streak mosaic virus (scsmv).	the complete nucleotide sequences of three sugarcane streak mosaic virus (scsmv) isolates found in sugarcane plants imported from japan (jp1, jp2) and indonesia (id) into china were determined. sequence analysis showed that, based on the whole genome sequence and the nucleotide sequences encoding the ten mature proteins, the three scsmv isolates shared identities of 81.62%-81.67% and 76.39%-85.93%, respectively, with a pakistan (pak) isolate of scsmv. phylogenetic analysis indicated that scsmv-j ...	2011	21927898
functional characterization of sugarcane myb transcription factor gene promoter (pscmybas1) in response to abiotic stresses and hormones.	the sugarcane (saccharum officinarum) stress-related myb transcription factor gene, scmybas1, demonstrated induced response to water deficit and salt stress in our previous study. to elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (pscmybas1, 1,033 bp) flanking the 5' scmybas1 coding region from the sugarcane genome. a series of pscmybas1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -8 ...	2011	22083650
sugarcane hsp101 is a hexameric chaperone that binds nucleotides.	the clp/hsp100 aaa+ chaperone family is involved in recovering aggregated proteins and little is known about other orthologs of the well studied clpb from escherichia coli and hsp104 from saccharomyces cerevisiae. plant hsp101 is a good model for understanding the relationship between the structure and function of hsp100 proteins and to investigate the role of these chaperones in disaggregation processes. here, we present the cloning and purification of a sugarcane ortholog, shsp101, which is ex ...	2011	21903129
acquisition and transmission of peanut clump virus by polymyxa graminis on cereal species.	the objective of this study was to investigate the specificity of the interactions between polymyxa graminis, peanut clump virus (pcv), and cereals, particularly the acquisition and the transmission of the virus by three p. graminis formae speciales. a new strategy has been developed: it involves using sugarcane as the common host for both the virus and its vector in order to produce the viruliferous zoospores of p. graminis f. sp. subtropicalis, temperata, and tropicalis that were then inoculat ...	2011	21916623
the actinobacterium microbacterium sp. 16sh accepts pbbr1-based pprobe vectors, forms biofilms, invades roots, and fixes n(2) associated with micropropagated sugarcane plants.	members of the genus microbacterium lineage of gram-positive actinobacteria are increasingly being reported to display significant traits associated with environmental biotechnology and bioengineering. 16sh is a nitrogen-fixing bacterial strain isolated from a surface-sterilized stem of sugarcane grown in guangxi, china. analysis of 16s rrna gene sequences revealed that 16sh belonged to the genus microbacterium. pprobe-ptet(r) plasmids were constructed by cloning the promoter region of the tet ...	2011	22002067
influence of nutritional and environmental factors on ethanol and endopolygalacturonase co-production by kluyveromyces marxianus ccebi 2011.	ethanol and endopolygalacturonase (endopg) are simultaneously produced by the yeast kluyveromyces marxianus ccebi 2011. the aim of this study was to determine the optimal combination of seven environmental and nutritional variables, as well as the influence of each one, with respect to the fermentation process in yeast cultures in which sugarcane juice was the substrate. simplex sequential optimization showed that after 15 runs the optimal conditions were: ph, 4.6; temperature, 31 ºc; total redu ...	2011	22015701
sugarcane biopolymer patch in femoral vein angioplasty on dogs.	to evaluate the use of the sugarcane biopolymer membrane in femoral vein patch angioplasty on dogs.	2011	21890306
Cellulase production from agricultural residues by recombinant fusant strain of a fungal endophyte of the marine sponge Latrunculia corticata for production of ethanol.	Several fungal endophytes of the Egyptian marine sponge Latrunculia corticata were isolated, including strains Trichoderma sp. Merv6, Penicillium sp. Merv2 and Aspergillus sp. Merv70. These fungi exhibited high cellulase activity using different lignocellulosic substrates in solid state fermentations (SSF). By applying mutagenesis and intergeneric protoplast fusion, we have obtained a recombinant strain (Tahrir-25) that overproduced cellulases (exo-ß-1,4-glucanase, endo-ß-1,4-glucanase and ß-1,4 ...	2011	21898149
hydrothermal pretreatment of sugarcane bagasse using response surface methodology improves digestibility and ethanol production by ssf.	sugarcane bagasse was characterized as a feedstock for the production of ethanol using hydrothermal pretreatment. reaction temperature and time were varied between 160 and 200°c and 5-20 min, respectively, using a response surface experimental design. the liquid fraction was analyzed for soluble carbohydrates and furan aldehydes. the solid fraction was analyzed for structural carbohydrates and klason lignin. pretreatment conditions were evaluated based on enzymatic extraction of glucose and xylo ...	2011	22080307
Establishment of regeneration and transformation system of sugarcane cultivar GT54-9 (C9).	Plant regeneration protocols for sugarcane GT54-9(C9) cultivar were developed for direct organogenesis and indirect somatic embryogenesis, using young leaf segments as explants by studying the influence of different concentrations and types of cytokinin and auxin hormones. For the callus formation from young leaves, a medium containing 4mg/l 2,4-D was found very effective. For embryo formation, MS medium supplemented with 1mg/l Kin and 0.5 mg/l 2,4-D was used. While in the case of direct organog ...	2011	21912218
Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control.	Yeasts associate with numerous insects, and they can assist the metabolic processes within their hosts. Two distinct yeasts were identified by PCR within the planthopper Perkinsiella saccharicida, the vector of Fiji disease virus to sugarcane. The utility of both microbes for potential paratransgenic approaches to control Fiji leaf gall (FLG) was assessed. Phylogenetic analysis showed one of the microbes is related to yeast-like symbionts from the planthoppers: Laodelphax striatellus, Nilaparvat ...	2011	21850475
Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis.	Effects of pretreatments with a white rot fungus, Ceriporiopsis subvermispora, and microwave hydrothermolysis of bagasse on enzymatic saccharification and fermentation were evaluated. The best sugar yield, 44.9 g per 100g of bagasse was obtained by fungal treatments followed by microwave hydrothermolysis at 180°C for 20 min. Fluorescent-labeled carbohydrate-binding modules which recognize crystalline cellulose (CjCBM3-GFP), non-crystalline cellulose (CjCBM28-GFP) and xylan (CtCBM22-GFP) were app ...	2011	21903385
isolation of a gastroprotective arabinoxylan from sugarcane bagasse.	after industrial processing, one-third of sugarcane culms is converted into residual bagasse. the xylan-rich hemicellulose components of the bagasse were extracted with hot aqueous alkali (ax-crude). approximately 82% of the extracted hemicelluloses was precipitated with ethanol (ax-pet). both ax-crude and ax-pet contained an arabinoxylan as confirmed by 13c nmr and methylation analysis. fraction ax-pet was fed to female wistar rats with ethanol-induced gastric lesions. oral administrations of 3 ...	2011	21945160
characterization of chloroplast dna microsatellites from saccharum spp and related species.	microsatellites, or simple sequence repeats (ssrs), and their flanking regions in chloroplast genomes (plastomes) of some species of the family poaceae were analyzed in silico to look for dna sequence variations. comparison of the complete chloroplast dna sequences (cpdnas) of sugarcane (saccharum hybrid cv. sp-80-3280 and s. officinarum cv. nco310) and related species, agrostis stolonifera, brachypodium distachyon, hordeum vulgare subsp vulgare, lolium perenne, oryza nivara, o. sativa sub ...	2011	21948764
microbial diversity in the larval gut of field and laboratory populations of the sugarcane weevil sphenophorus levis (coleoptera, curculionidae).	the sugarcane weevil, sphenophorus levis, is a wide-spread sugarcane pest in brazil. sphenophorus levis may depend on microorganisms that inhabit its intestinal tract. we examined the diversity of the gut microbiota of s. levis, which was characterized using culture-dependent and culture-independent methods. analysis of 16s rrna amplified directly from the gut community revealed the presence of 14 genera, one group from the candidatus category, one uncultured group assigned to the family f ...	2011	22057964
a comparative study of ph modulation and trace elements of various fruit juices on enamel erosion: an in vitro study.	fruit juices are popular worldwide with children of all ages as they are sweet and perceived to be healthful. this in vitro study was sought to measure ph of 10 different fruit juices, to find out possible erosive effects on human dental enamel of 40 extracted sound premolars and also to measure fluoride and trace elements of these juices. the estimation of ph of fruit juices was done by using systronic uph 362 ph meter. the erosive effects of fruit juices were tested by using polarized light mi ...	2011	21985876
isomaltulose is actively metabolized in plant cells.	isomaltulose is a structural isomer of sucrose (suc). it has been widely used as a nonmetabolized sugar in physiological studies aimed at better understanding the regulatory roles and transport of sugars in plants. it is increasingly used as a nutritional human food, with some beneficial properties including low glycemic index and acariogenicity. cloning of genes for suc isomerases opened the way for direct commercial production in plants. the understanding that plants lack catabolic capabilitie ...	2011	22010106
comparative study of the areas of osteochondral defects produced in the femoral condyles of rabbits treated with gel of sugarcane biopolymer.	to measure the healed areas of osteochondral defects produced in femoral condyles of rabbits filled with biopolymer sugar cane gel and to compare these with those of the control group at 90, 120 and 180 days.	2011	21952662
Molecular Identification of Fusarium Species in Gibberella fujikuroi Species Complex from Rice, Sugarcane and Maize from Peninsular Malaysia.	The objective of this study was to identify Fusarium species in the Gibberella fujikuroi species complex from rice, sugarcane and maize as most of the Fusarium species in the species complex are found on the three crops. Isolates used were collected from the field and obtained from culture collection. The Fusarium isolates were initially sorted based on morphology and identifications confirmed based on the DNA sequence of the translation elongation factor 1-a (TEF-1a) gene. Based on the closest ...	2011	22072914
enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of pichia kudriavzevii.	the thermotolerant yeast strain isolated from sugarcane juice through enrichment technique was identified as a strain of pichiakudriavzevii (issatchenkiaorientalis) through molecular characterization. the p. kudriavzevii cells adapted to galactose medium produced about 30% more ethanol from sugarcane juice than the non-adapted cells. the recycled cells could be used for four successive cycles without a significant drop in ethanol production. fermentation in a laboratory fermenter with galactose ...	2011	21398115
peroxisomal polyhydroxyalkanoate biosynthesis is a promising strategy for bioplastic production in high biomass crops.	polyhydroxyalkanoates (phas) are bacterial carbon storage polymers with diverse plastic-like properties. pha biosynthesis in transgenic plants is being developed as a way to reduce the cost and increase the sustainability of industrial pha production. the homopolymer polyhydroxybutyrate (phb) is the simplest form of these biodegradable polyesters. plant peroxisomes contain the substrate molecules and necessary reducing power for phb biosynthesis, but peroxisomal phb production has not been explo ...	2011	21447054
biodegradation of basic violet 3 by candida krusei isolated from textile wastewater.	basic violet 3 (bv) belongs to the most important group of synthetic colorants and is used extensively in textile industries. it is considered as xenobiotic compound which is recalcitrant to biodegradation. as candida krusei could not use bv as sole carbon source, experiments were conducted to study the effect of cosubstrates on decolorization of bv in semi synthetic medium using glucose, sucrose, lactose, maltose, yeast extract, peptone, urea and ammonium sulphate. maximum decolorization (74%) ...	2011	21479732
mutagenic activity of airborne particulate matter (pm(10)) in a sugarcane farming area (araraquara city, southeast brazil).	brazil contains 25% of the total land planted with sugarcane in the world and is thus one of the major producers. the annual burning of sugarcane fields prior to harvesting emits huge amounts of pyrogenic particles. biomass burning is an important primary and secondary source of aerosol particles. the presence of carbonaceous particles in the inhalable size range makes it important to study this fraction in view of the possible effects on human health and the climate. in this study, the mutageni ...	2011	21481367
biological control of red rot in sugarcane by native pyoluteorin-producing pseudomonas putida strain nh-50 under field conditions and its potential modes of action.	background: rhizobacteria have a good potential to suppress soilborne diseases, but their efficacy against sugarcane pests is rarely reported. bacterial strains isolated from the rhizosphere of sugarcane were evaluated for their potential to suppress red rot disease on two susceptible varieties, co-1148 and spf-234, under field conditions. the strains were also characterised for the production of secondary metabolites associated with their antagonistic activity. results: one out of four strains, ...	2011	21495154
production of sophorolipid glycolipid biosurfactants from sugarcane molasses using starmerella bombicola nbrc 10243.	biosurfactants (bs) are produced by a variety of microorganisms from renewable resources, and have unique properties compared to chemical surfactants. in order to attain efficient production of bs from low-cost materials, we focused our attention on the use of sugarcane molasses. fifteen yeast strains that are known as bs producers were examined for bs productivity from a culture medium consisting of only molasses and water. among the strains tested, only starmerella bombicola nbrc 10243 produce ...	2011	21502725
injection of air into the headspace improves fermentation of phosphoric acid pretreated sugarcane bagasse by escherichia coli mm170.	microaeration (injecting air into the headspace) improved the fermentation of hemicellulose hydrolysates obtained from the phosphoric acid pretreatment of sugarcane bagasse at 170°c for 10min. in addition, with 10% slurries of phosphoric acid pretreated bagasse (180°c, 10min), air injection into the headspace promoted xylose utilization and increased ethanol yields from 0.16 to 0.20g ethanol/g bagasse dry weight using a liquefaction plus simultaneous saccharification and co-fermentation process ...	2011	21531547
molecular analysis of the complete genomic sequences of four isolates of gooseberry vein banding associated virus.	the presence of gooseberry vein banding associated virus (gvbav), a badnavirus in the family caulimoviridae, is strongly correlated with gooseberry vein banding disease in ribes spp. in this study, full-length genomic sequences of four gvbav isolates from different hosts and geographic regions were determined to be 7649-7663 nucleotides. these isolates share identities of 96.4-97.3% for the complete genomic sequence, indicating low genetic diversity among them. the gvbav genome contains three op ...	2011	21533750
f(2) screening for resistance to pyramided bacillus thuringiensis maize in louisiana and mississippi populations of diatraea saccharalis (lepidoptera: crambidae).	background: in the mid-southern region of the united states, sugarcane borer, diatraea saccharalis (f.), is a major target pest of transgenic maize expressing bacillus thuringiensis (bt) proteins. novel transgenic maize technologies containing two or more pyramided bt genes for controlling lepidopteran pests have recently become commercially available. insect resistance management (irm) is an important issue in the sustainable use of bt crop technologies. the objective of this study was to deter ...	2011	21538799
quantitative proteomic analysis of the interaction between the endophytic plant-growth-promoting bacterium gluconacetobacter diazotrophicus and sugarcane.	gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium that colonizes sugarcane. in order to investigate molecular aspects of the g. diazotrophicus-sugarcane interaction, we performed a quantitative mass spectrometry-based proteomic analysis by (15)n metabolic labeling of bacteria, root samples, and co-cultures. overall, more than 400 proteins were analyzed and 78 were differentially expressed between the plant-bacterium interaction model and control cultures. a comparative analy ...	2011	21190439
identification of new candidate pathogenicity factors in the xylem-invading pathogen xanthomonas albilineans by transposon mutagenesis.	xanthomonas albilineans is a xylem-invading pathogen that produces the toxin albicidin that blocks chloroplast differentiation, resulting in disease symptoms of sugarcane leaf scald. in contrast to other xanthomonads, x. albilineans does not possess a hypersensitive response and pathogenicity type iii secretion system and does not produce xanthan gum. albicidin is the only previously known pathogenicity factor in x. albilineans, yet albicidin-deficient mutant strains are still able to efficientl ...	2011	21190440
the genome sequence of e. coli w (atcc 9637): comparative genome analysis and an improved genome-scale reconstruction of e. coli.	escherichia coli is a model prokaryote, an important pathogen, and a key organism for industrial biotechnology. e. coli w (atcc 9637), one of four strains designated as safe for laboratory purposes, has not been sequenced. e. coli w is a fast-growing strain and is the only safe strain that can utilize sucrose as a carbon source. lifecycle analysis has demonstrated that sucrose from sugarcane is a preferred carbon source for industrial bioprocesses.	2011	21208457
biotechnology tools in agriculture: recent patents involving soybean, corn and sugarcane.	the technological opportunities opened up by biotechnology in agriculture are diverse, including plant breeding, the partial or total relief of pesticides chemicals usage, the improvement of soil fertility, the improvement of the quality attributes of various foods. specifically, various tricks of biotechnology can be used for higher seed yield, resistance to diseases and insects, better stems and roots, tolerance to drought and heat, and better agronomic quality. a number of recent works consid ...	2011	21226663
short distance movement of genomic negative strands in a host and nonhost for sugarcane mosaic virus (scmv).	in order to obtain an initial and preliminary understanding of host and nonhost resistance in the initial step of potyvirus replication, both positive and negative sugarcane mosaic virus (scmv) strands where traced in inoculated and systemic leaves in host and nonhost resistant maize and sugarcane for one mexican potyviral isolate (scmv-ver1). intermediary replication forms, such as the negative viral strand, seem to only move a short distance as surveyed by rt-pcr analysis and elisa in differen ...	2011	21232133
digestive physiology and characterization of digestive cathepsin l-like proteinase from the sugarcane weevil sphenophorus levis.	sugarcane is an important crop that has recently become subject to attacks from the weevil sphenophorus levis, which is not efficiently controlled with chemical insecticides. this demands the development of new control devices for which digestive physiology data are needed. in the present study, ion-exchange chromatography of s. levis whole midgut homogenates, together with enzyme assays with natural and synthetic substrates and specific inhibitors, demonstrated that a cysteine proteinase is a m ...	2011	21256130
production of astaxanthin from cellulosic biomass sugars by mutants of the yeast phaffia rhodozyma.	astaxanthin is a potential high-value coproduct in an ethanol biorefinery. three mutant strains of the astaxanthin-producing yeast phaffia rhodozyma, which were derived from the parent strain atcc 24202 (ucd 67-210) and designated jtm166, jtm185, and ssm19, were tested for their capability of utilizing the major sugars that can be generated from cellulosic biomass, including glucose, xylose, and arabinose, for astaxanthin production. while all three strains were capable of metabolizing these sug ...	2011	21274657
use of soybean vinasses as a germinant medium for a geobacillus stearothermophilus atcc 7953 sterilization biological indicator.	a novel low-cost medium was developed from by-products and wastes from the ethanol agro-industry to replace commercial media in the production of a steam sterilization biological indicator (bi). various recovery media were developed using soybean or sugarcane molasses and vinasse to prepare a self-contained bi. media performance was evaluated by viability and heat resistance (d(121 °c) value) according to regulatory standards. a medium produced with a soybean vinasse ratio of 1:70 (1.4%) (w/v) p ...	2011	21336685
[sugarcane biopolymer membrane: experimental evaluation in the middle ear].	new developments on biomaterials are important in surgery. the behavior of a new membrane produced from sugarcane will be evaluated in the middle ear of rats.	2011	21340188
effect of reduced sulfur compounds on the fermentation of phosphoric acid pretreated sugarcane bagasse by ethanologenic escherichia coli.	the addition of reduced sulfur compounds (thiosulfate, cysteine, sodium hydrosulfite, and sodium metabisulfite) increased growth and fermentation of dilute acid hydrolysate of sugarcane bagasse by ethanologenic escherichia coli (strains ly180, emfr9, and mm160). with sodium metabisulfite (0.5mm), toxicity was sufficiently reduced that slurries of pretreated biomass (10% dry weight including fiber and solubles) could be fermented by e. coli strain mm160 without solid-liquid separation or cleanup ...	2011	21353535
immunotherapeutic effects of some sugar cane (saccharum officinarum l.) extracts against coccidiosis in industrial broiler chickens.	present paper reports the effects of aqueous and ethanolic extracts of sugar cane (saccharum officinarum l.) juice and bagasse, respectively on protective immune responses in industrial broiler chickens against coccidiosis. immunotherapeutic efficacies of the extracts were measured by evaluating their effect on body weight gain, oocyst shedding, lesion score, anti-coccidial indices, per cent protection and elicited serum antibody responses against coccidiosis. results revealed a significantly lo ...	2011	21354144
d(-)-lactic acid production by leuconostoc mesenteroides b512 using different carbon and nitrogen sources.	sugar concentration from sugarcane juice and yeast autolysate increased lactic acid production more than the other agro-industrial substrates tested. the concentrations of these two components were further optimized using the plackett-burman design and response surface method. a second-order polynomial regression model estimated that a maximal lactic acid production of 66.11 g/l would be obtained when the optimal values of sugar and yeast autolysate were 116.9 and 44.25 g/l, respectively. to val ...	2011	21360091
anaerobic thermophilic fermentation for carboxylic acid production from in-storage air-lime-treated sugarcane bagasse.	wet storage and in situ lime pretreatment (50 °c, 1-atm air, 56 days, excess lime loading of 0.3 g ca(oh)(2)/g dry biomass) of sugarcane bagasse (4,000 g dry weight) was performed in a bench-scale pile pretreatment system. under thermophilic conditions (55 °c, nh(4)hco(3) buffer, methane inhibitors), air-lime-treated bagasse (80 wt.%) and chicken manure (20 wt.%) were anaerobically co-digested in 1-l rotary fermentors by a mixed culture of marine microorganisms (galveston, tx). during four-stage ...	2011	21365471
duplex-immunocapture-rt-pcr for detection and discrimination of two distinct potyviruses naturally infecting sugarcane (saccharum spp. hybrid).	a sensitive duplex-immunocapture-rt-pcr (d-ic-rt-pcr) technique was developed for detection and discrimination of taxonomically distinct sugarcane streak mosaic virus (scsmv) and sugarcane mosaic virus (scmv) that naturally infect sugarcane. d-ic-rt-pcr was performed using polyclonal antisera for capture of virions. oligo 5'-d(t)18(agc)-3' as a common reverse primer for both viruses and virus specific forward primers, 5'-aagtggttaaacgcctgtgg-3' and 5'-atgtc(ga)aagaa(ga)atgcgcttgc-3' were used fo ...	2011	21365999
improvement of ethanol production using saccharomyces cerevisiae by enhancement of biomass and nutrient supplementation.	optimization of ethanol production through addition of substratum and protein-lipid additives was studied. oilseed meal extract was used as protein lipid supplement, while rice husk was used as substratum. the effect of oil seed meal extract and rice husk was observed at varying concentration of medium sugar from 8% to 20%. of the three oil seed meal extracts used, viz. groundnut, safflower, and sunflower, safflower was found to be most efficient. the use of oilseed meal extract at 4% was found ...	2011	21373793
laboratory and field evaluation of metarhizium anisopliae var. anisopliae for controlling subterranean termites.	the efficacy of the metarhizium anisopliae strain arsef 6911 was determined in the laboratory and field against two sugarcane pests, microtermes obesi holmgren and odontotermes obesus rambur (termitidae: isoptera). the susceptibility of both termite species to different conidial suspensions (1 × 10(10), 1 × 10(8), 1 × 10(6) and 1 × 10(4) conidia/ml) was determined in laboratory. all conidial suspensions were able to induce mortality. termite mortality caused by the fungal suspensions was dose de ...	2011	21584407
global warming, plant paraquat resistance, and light signal transduction through nucleoside diphosphate kinase as a paradigm for increasing food supply.	light signal transduction was studied in extracts of mycelia of the fungus neurospora crassa, and the third internodes of dark-grown pisum sativum cv alaska. both processes increased the phosphorylation of nucleoside diphosphate kinase (ndpk). ndpk may function as a carrier of reduction equivalents, as it binds nadh, thereby providing electrons to transform singlet oxygen to superoxide by catalases (cat). as the c-termini of ndpk interact with cat which receive singlet oxygen, emitted from photo ...	2011	21603975
isolation of pseudozyma churashimaensis sp. nov., a novel ustilaginomycetous yeast species as a producer of glycolipid biosurfactants, mannosylerythritol lipids.	an ustilaginomycetous anamorphic yeast species isolated from the leaves of saccharum officinarum (sugarcane) in okinawa, japan, was identified as a novel pseudozyma species based on morphological and physiological aspects and molecular taxonomic analysis using the d1/d2 domains of the large subunit (26s) rrna gene and the internal transcribed spacer 1 (its1)-5.8s-its2 regions. the name pseudozyma churashimaensis sp. nov. was proposed for the novel species, with jcm 16988(t) as the type strain. i ...	2011	21606002
candida middelhoveniana sp. nov., a new yeast species found on the rhizoplane of organically cultivated sugarcane.	a novel yeast species within the metschnikowiaceae is described based on a strain from the sugarcane (saccharum sp.) rhizoplane of an organically managed farm in rio de janeiro, brazil. the d1/d2 domain of the large subunit ribosomal rna gene sequence analysis showed that the closest related species were candida tsuchiyae with 86.2% and candida thailandica with 86.7% of sequence identity. all three are anamorphs in the clavispora opuntiae clade. the name candida middelhoveniana sp. nov. is propo ...	2011	21614495
ethanol production by a new pentose-fermenting yeast strain, scheffersomyces stipitis ufmg-imh 43.2, isolated from the brazilian forest.	the ability of a recently isolated scheffersomyces stipitis strain (ufmg-imh 43.2) to produce ethanol from xylose was evaluated. for the assays, a hemicellulosic hydrolysate produced by dilute acid hydrolysis of sugarcane bagasse was used as the fermentation medium. initially, the necessity of adding nutrients (mgso(4) ·7h(2) o, yeast extract and/or urea) to this medium was verified, and the yeast extract supplementation favoured ethanol production by the yeast. then, in a second stage, assays u ...	2011	21626536
co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus for simultaneous biosynthesis of xylanase and laccase under solid-state fermentation.	co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus mtcc 1804 was evaluated for the production of xylanase-laccase mixture under solid-state fermentation (ssf) condition. growth compatibility between mutant p. oxalicum sau(e)-3.510 and white rot fungi (p. ostreatus mtcc 1804, trametes hirsuta mtcc 136 and pycnoporus sp. mtcc 137) was analyzed by growing them on potato dextrose agar plate. extracellular enzyme activities were determined spectrophotometrically. under ...	2011	21642024
optimization of fibrolytic enzyme production by aspergillus japonicus c03 with potential application in ruminant feed and their effects on tropical forages hydrolysis.	fibrolytic enzyme production by aspergillus japonicus c03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. a 2(3) full factorial composite and response surface methodology were used to design the experiments and analysis of results. tropical forages were hydrolyzed by a. japonicus c03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. optimal production to xylanase was obtained with soybean bran added ...	2011	21647681
responses of maize (zea mays l.) near isogenic lines carrying wsm1, wsm2, and wsm3 to three viruses in the potyviridae.	genes on chromosomes six (wsm1), three (wsm2) and ten (wsm3) in the maize (zea mays l.) inbred line pa405 control resistance to wheat streak mosaic virus (wsmv), and the same or closely linked genes control resistance to maize dwarf mosaic virus (mdmv) and sugarcane mosaic virus (scmv). near isogenic lines (nil) carrying one or two of the genes were developed by introgressing regions of the respective chromosomes into the susceptible line oh28 and tested for their responses to wsmv, mdmv, and sc ...	2011	21667271
nutrient budgets (c, n and p) and trophic dynamics of a brazilian tropical estuary: barra das jangadas.	this paper focuses on the nutrient dynamics of a tropical estuary on the northeastern brazilian coast, studied using the loicz biogeochemical budgeting protocol. we describe the methodology and assumptions underlying this model. input data (monthly for rainfall, evaporation, river discharge, and concentrations of salt, phosphorus and nitrogen) were obtained during field campaigns in the barra das jangadas estuary (bje) over a 5 years period (1999 to 2003). mass balance results indicate large inp ...	2011	21670871
bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil.	a gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated sc-n012(t), was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. the strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus bacillus. sequence analysis of the 16s rrna gene of sc-n012(t) revealed the closest match (98.9% pair wise similarity) with bacillus clausii dsm 8716(t). however, dna- ...	2011	21671194
differential gene expression in sugarcane in response to challenge by fungal pathogen ustilago scitaminea revealed by cdna-aflp.	differential gene expression in sugarcane during sugarcane-ustilago scitaminea interaction was conducted in a smut-resistant genotype. using cdna-aflp along with silver staining, a total of 136 transcript-derived fragments (tdfs) were found to be differentially expressed in response to challenge by u. scitaminea. forty tdfs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by rt-pcr analysis. these results demonstrated that the expressio ...	2011	21792273
sugarcane glycoproteins may act as signals for the production of xanthan in the plant-associated bacterium xanthomonas albilineans.	visual symptoms of leaf scald necrosis in sugarcane (saccharum officinarum) leaves develop in parallel to the accumulation of a fibrous material invading exocellular spaces and both xylem and phloem. these fibers are produced and secreted by the plant-associated bacterium xanthomonas albilineans. electron microscopy and specific staining methods for polysaccharides reveal the polysaccharidic nature of this material. these polysaccharides are not present in healthy leaves or in those from disease ...	2011	21791980
chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice.	chronic ethanol consumption is a major public health problem throughout the world. we investigated the anxiolytic-like effects and the possible ever injury induced by the chronic consumption of ethanol or sugarcane spirit in mice.	2011	21789394
development of a low-cost sterilization biological indicator using bacillus atrophaeus by solid-state fermentation.	the production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. the goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. the performance of three different inexpensive supports (vermiculite, sand, and sugarcane bagasse) was assessed by determining bacillus atrophaeus sporulation during solid-state fermentation and by assessing the direct u ...	2011	21785930
lipid production from yarrowia lipolytica po1g grown in sugarcane bagasse hydrolysate.	this study investigated the possibility of utilizing detoxified sugarcane bagasse hydrolysate (dscbh) as an alternative carbon source to culture yarrowia lipolytica po1g for microbial oil and biodiesel production. sugarcane bagasse hydrolysis with 2.5% hcl resulted in maximum total sugar concentration (21.38g/l) in which 13.59g/l is xylose, 3.98g/l is glucose, and 2.78g/l is arabinose. detoxification of scbh by ca(oh)(2) neutralization reduced the concentration of 5-hydroxymethylfurfural and fur ...	2011	21757339
poly-lactic acid synthesis for application in biomedical devices - a review.	bioabsorbable polymers are considered a suitable alternative to the improvement and development of numerous applications in medicine. poly-lactic acid (pla,) is one of the most promising biopolymers due to the fact that the monomers may produced from non toxic renewable feedstock as well as is naturally occurring organic acid. lactic acid can be made by fermentation of sugars obtained from renewable resources as such sugarcane. therefore, pla is an eco-friendly product with better features for u ...	2011	21756992
leviserpin: a serine peptidase inhibitor (serpin) from the sugarcane weevil sphenophorus levis.	serine peptidase inhibitors (serpins) form a superfamily of proteins covering abroad spectrum of different biological functions. here we describe the inhibitory characterization of leviserpin, the first serpin from the sugar cane weevil sphenophorus levis. leviserpin was able to inhibit bovine trypsin by the formation of the covalent complex serpin-peptidase, demonstrated by sds-page and mass spectroscopy analysis. we also have determined the cleavage site at the reactive center loop, by the ana ...	2011	21748377
production of bioethanol, methane and heat from sugarcane bagasse in a biorefinery concept.	the potential of biogas production from the residues of second generation bioethanol production was investigated taking into consideration two types of pretreatment: lime or alkaline hydrogen peroxide. bagasse was pretreated, enzymatically hydrolyzed and the wastes from pretreatment and hydrolysis were used to produce biogas. results have shown that if pretreatment is carried out at a bagasse concentration of 4% dm, the highest global methane production is obtained with the peroxide pretreatment ...	2011	21689929
high molecular variability of sugarcane bacilliform viruses in guadeloupe implying the existence of at least three new species.	thirty-five unique partial sugarcane bacilliform virus (scbv) sequences extending over 529bp were identified in sugarcane samples from guadeloupe diagnosed by immunocapture-pcr (ic-pcr) using specific badnavirus primers. phylogenetic analysis of these sequences along with the two known genome sequences of sugarcane bacilliform mor virus (scbmv) and sugarcane bacilliform im virus (scbimv) revealed high molecular variability in the scbv genome. seven phylogenetic groups, named a to g, were charact ...	2011	21741419
effects of d-003 (10 mg/day) on bone mineral density of the lumbar spine and femoral neck in postmenopausal women: a randomized, double-blinded study.	increased osteoclast activity is a pivotal finding in osteoporosis. this increase is mediated via the mevalonate-to-cholesterol pathway, which is involved in producing the intermediates required for osteoclast activity. d-003, a mixture of high molecular weight sugarcane wax acids, has been shown to inhibit cholesterol synthesis prior to mevalonate production, resulting in a reduction of bone loss and resorption in ovariectomized rats. moreover, previous studies have demonstrated that short-term ...	2011	21716593
plasmodium falciparum and helminth coinfections among schoolchildren in relation to agro-ecosystems in mvomero district, tanzania.	in sub-saharan africa, some individuals infected with malaria are also infected with helminths. however, the magnitude and distribution of such coinfections in relation to eco-systems remains poorly defined. this study was undertaken to determine the prevalence of plasmodium falciparum and helminth coinfections among schoolchildren in relation to agro-ecosystems in mvomero district, tanzania.	2011	21741929
production and characterization of a glycolipid biosurfactant, mannosylerythritol lipid b, from sugarcane juice by ustilago scitaminea nbrc 32730.	mannosylerythritol lipids (mels) are glycolipid biosurfactants excreted by fungal strains. they show not only excellent surface-active properties but also versatile biochemical actions. ustilago scitaminea nbrc 32730 has been reported mainly to produce a mono-acetylated and di-acylated mel, mel-b, from sucrose as sole carbon source. in order to make biosurfactant production more efficient, we focused our attention on the use of sugarcane juice, one of the most economical resources. the fungal st ...	2011	21737925
complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of sugarcane mosaic virus.	sugarcane mosaic virus (scmv) is the most prevalent virus causing maize dwarf mosaic disease in northern china. a scmv isolate, bd8, was obtained from the maize showing dwarf and mosaic symptoms in baoding, china. the complete genomic sequence of bd8 is 9,576 nucleotides (nt) excluding the poly(a) tail. it contains one single open reading frame of 9,192-ánt and encodes a large polyprotein of 3,063-áamino acids (aa), flanked by a 5'-untranslated region (utr) of 148-ánt and a 3'-utr of 236-ánt. th ...	2011	21833715
differential protein expression in sugarcane during sugarcane-sporisorium scitamineum interaction revealed by 2-de and maldi-tof-tof/ms.	to understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant proteins that respond to the pathogen attack. two sugarcane varieties, nco376 and ya71-374, were used in this study. by applying 2-dimensional electrophoresis (2-de), the protein expression profile of sugarcane after inoculating with sporisorium scitamineum was analyzed. in total, 23 differentially expressed proteins were identified by maldi-tof-tof/ms. bioinformatics analysis revealed t ...	2011	21822403
scientific challenges of bioethanol production in brazil.	bioethanol (fuel alcohol) has been produced by industrial alcoholic fermentation processes in brazil since the beginning of the twentieth century. currently, 432 mills and distilleries crush about 625 million tons of sugarcane per crop, producing about 27 billion liters of ethanol and 38.7 million tons of sugar. the production of bioethanol from sugarcane represents a major large-scale technology capable of producing biofuel efficiently and economically, providing viable substitutes to gasoline. ...	2011	21735264
choice of macroinvertebrate metrics to evaluate stream conditions in atlantic forest, brazil.	the development of biomonitoring programs based on the macroinvertebrate community requires the understanding of species distribution patterns, as well as of the responses of the community to anthropogenic stressors. in this study, 49 metrics were tested as potential means of assessing the condition of 29 first- and second-order streams located in areas of differing types of land use in são paulo state, brazil. of the sampled streams, 15 were in well-preserved regions in the atlantic forest, 5 w ...	2011	20461547
transgenic expression of trehalulose synthase results in high concentrations of the sucrose isomer trehalulose in mature stems of field-grown sugarcane.	sugarcane plants were developed that produce the sucrose isomers trehalulose and isomaltulose through expression of a vacuole-targeted trehalulose synthase modified from the gene in 'pseudomonas mesoacidophila mx-45' and controlled by the maize ubiquitin (ubi-1) promoter. trehalulose concentration in juice increased with internode maturity, reaching about 600 mm, with near-complete conversion of sucrose in the most mature internodes. plants remained vigorous, and trehalulose production in select ...	2011	20492546
genetic transformation with untranslatable coat protein gene of sugarcane yellow leaf virus reduces virus titers in sugarcane.	sugarcane yellow leaf syndrome, characterized by a yellowing of the leaf midrib followed by leaf necrosis and growth suppression, is caused by sugarcane yellow leaf virus (scylv). we produced scylv-resistant transgenic sugarcane from a susceptible cultivar (h62-4671) and determined the amount of virus present following inoculation. the transgenic plants were produced through biolistic bombardment of cell cultures with an untranslatable coat protein gene. presence of the transgene in regenerated ...	2011	20661641
nutritional value of sugarcane silage enriched with corn grain, urea, and minerals as feed supplement on growth performance of beef steers grazing stargrass.	the objectives were to evaluate the silage quality of sugarcane silage enriched (as fed) with corn grain ground (10%), urea (1.5%), and mineral premix (0.5%) and its effects as a feed supplement on growth performance of beef steers grazing stargrass. firstly, in micro-silages, whole sugarcane enriched with corn grain, urea, and minerals (wsce) were ensilaged by 0, 20, 40, and 60 days. crude protein (cp) and lactic acid (la) increased linearly (p < 0.05) and true protein decreased linearly (p < 0 ...	2011	20711753
production of cellulases and xylanases under catabolic repression conditions from mutant pr-22 of cellulomonas flavigena.	derepressed mutant pr-22 was obtained by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) mutagenic treatment of cellulomonas flavigena pn-120. this mutant improved its xylanolytic activity from 26.9 to 40 u mg(-1) and cellulolytic activity from 1.9 to 4 u mg(-1); this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. either glucose or cellobiose was added to cultures of c. flavigena pn-120 and mutant pr-22 induced with sugarcan ...	2011	20803244
kinetics of lime pretreatment of sugarcane bagasse to enhance enzymatic hydrolysis.	the objective of this work was to determine the optimum conditions of sugarcane bagasse pretreatment with lime to increase the enzymatic hydrolysis of the polysaccharide component and to study the delignification kinetics. the first stage was an evaluation of the influence of temperature, reaction time, and lime concentration in the pretreatment performance measured as glucose release after hydrolysis using a 2(3) central composite design and response surface methodology. the maximum glucose yie ...	2011	20803263
pretreatment of synthetic dairy wastewater using the sophorolipid-producing yeast candida bombicola.	the presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. in the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-producing yeast candida bombicola in a laboratory-scale bioreactor under batch, fed-batch, and continuous modes of operation. to support the yeast growth, the wastewater was suppleme ...	2011	20821070
bioconversion of novel substrate saccharum spontaneum, a weedy material, into ethanol by pichia stipitis ncim3498.	rising fuel prices and environmental issues have paved the way for the exploration of cellulosic ethanol. however, challenges involving substrate hydrolysis and cost-effectiveness still limit the efficient bioconversion and utilization of cellulosic ethanol. we aimed to evaluate a cheaper and abundantly available wild sugarcane variety, saccharum spontaneum, as the raw substrate for bioconversion of ethanol by pichia stipitis ncim3498. three different strategies for substrate hydrolysis using ac ...	2011	20822899
improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents.	inhibitory lignocellulose hydrolysates were treated with the reducing agents dithionite and sulfite to achieve improved fermentability. addition of these reducing agents (in the concentration range 5.0-17.5 mm) to enzymatic hydrolysates of spruce wood or sugarcane bagasse improved processes based on both shf (simultaneous hydrolysis and fermentation) and ssf (simultaneous saccharification and fermentation). the approach was exemplified in ethanolic fermentations with saccharomyces cerevisiae and ...	2011	20822900
biological detoxification of different hemicellulosic hydrolysates using issatchenkia occidentalis cctcc m 206097 yeast.	this work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using issatchenkia occidentalis cctcc m 206097 yeast. tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentrati ...	2011	20844925
structural characteristics and phylogenetic analysis of the mitochondrial genome of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae).	the sugarcane borer, diatraea saccharalis (f.), is an economically important pest of several major crops in north and south america. there is great concern over potential resistance development to the valuable transgenic bacillus thuringiensis corn. cost-effective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field populations of d. saccharalis, are needed. this article reports the complete mitochondrial genome sequen ...	2011	20849253
genomic and evolutionary features of the spi-1 type iii secretion system that is present in xanthomonas albilineans but is not essential for xylem colonization and symptom development of sugarcane leaf scald.	xanthomonas albilineans is the causal agent of sugarcane leaf scald. interestingly, this bacterium, which is not known to be insect or animal associated, possesses a type iii secretion system (t3ss) belonging to the injectisome family salmonella pathogenicity island 1 (spi-1). the t3ss spi-1 of x. albilineans shares only low similarity with other available t3ss spi-1 sequences. screening of a collection of 128 plant-pathogenic bacteria revealed that this t3ss spi-1 is present in only two species ...	2011	20955079
bioethanol production from dedicated energy crops and residues in arkansas, usa.	globally, one of the major technologic goals is to achieve cost-effective lignocellulosic ethanol production from biomass feedstocks. lignocellulosic biomass of four dedicated energy crops [giant reed (arundo donax l.), elephantgrass (pennisetum purpureum (schumach), miscanthus × giganteus (illinois clone), and (clone q42641) {hybrid of miscanthus sinensis anderss. and miscanthus sacchariflorus (maxim)}, hack. called giant miscanthus, and sugarcane clone us 84-1028 (saccharum l. spp. hybrid)] an ...	2011	21086455
specific plant induced biofilm formation in methylobacterium species.	two endophytic strains of methylobacterium spp. were used to evaluate biofilm formation on sugarcane roots and on inert wooden sticks. results show that biofilm formation is variable and that plant surface and possibly root exudates have a role in methylobacterium spp. host recognition, biofilm formation and successful colonization as endophytes.	2011	24031703
draft genome sequences of xanthomonas sacchari and two banana-associated xanthomonads reveal insights into the xanthomonas group 1 clade.	we present draft genome sequences for three strains of xanthomonas species, each of which was associated with banana plants (musa species) but is not closely related to the previously sequenced banana-pathogen xanthomonas campestris pathovar musacearum. strain ncppb4393 had been deposited as xanthomonas campestris pathovar musacearum but in fact falls within the species xanthomonas sacchari. strain ncppb1132 is more distantly related to xanthomonas sacchari whilst strain ncppb 1131 grouped in a ...	2011	24710305
predominant nifh transcript phylotypes related to rhizobium rosettiformans in field-grown sugarcane plants and in norway spruce.	although some sugarcane cultivars may benefit substantially from biological nitrogen fixation (bnf), the responsible bacteria have been not identified yet. here, we examined the active diazotrophic bacterial community in sugarcane roots from africa and america by reverse transcription (rt)-pcr using broad-range nifh-specific primers. denaturing gradient gel electrophoresis (dgge) profiles obtained from sugarcane showed a low diversity at all sample locations with one phylotype amounting up to 10 ...	2011	23761284
holocellulase activity from schizophyllum commune grown on bamboo: a comparison with different substrates.	the natural biodiversity that is found in tropical areas offers countless biotechnological opportunities; especially if we take in account that many biomolecules from several microorganisms have supported for many years, different industrial applications in areas such as pharmacology, agro-industry, bioprocess, environmental technology, and bioconversion. in order to find new lignocellulolytic enzymes and evaluate bamboo fibers as substrate, schizophyllum commune a fungus with broad distribution ...	2011	21964940
quantitative proteomic analysis of lignocellulolytic enzymes by phanerochaete chrysosporium on different lignocellulosic biomass.	lignocellulosic biomass from agricultural crop residues and forest waste represents an abundant renewable resource for bioenergy and future biofuel. the current bottleneck of lignocellulosic biofuel production is the hydrolysis of biomass to sugar. to understand the enzymatic hydrolysis of complex biomasses, in this report, lignocellulolytic enzymes secretion by phanerochaete chrysosporium cultivated in different natural lignocellulosic biomass such as corn stover, hay, sawdust, sugarcane baggas ...	2011	22146477
efficient conversion of sugarcane stalks into ethanol employing low temperature alkali pretreatment method.	an alternative route for bio-ethanol production from sugarcane stalks (juice and bagasse) featuring a previously reported low temperature alkali pretreatment method was evaluated. test-tube scale pretreatment-saccharification experiments were carried out to determine optimal lta pretreatment conditions for sugarcane bagasse with regard to the efficiency of enzymatic hydrolysis of the cellulose. free fermentable sugars and bagasse recovered from 2 kg of sugarcane stalks were jointly converted int ...	2011	22000967
transcriptome analysis of aspergillus niger grown on sugarcane bagasse.	abstract:	2011	22008461
poly(3-hydroxybutyrate) production by bacillus cereus spv using sugarcane molasses as the main carbon source.	the main hindrance in the use of polyhydroxyalkanoates (phas) as a replacement for existing petroleum-based plastics is their high production cost. the carbon source accounts for 50% of the cost for pha production. thus, increasing the yield and productivity of phas on cheap substrates is an important challenge for biotechnologists to support the commercialization and further applications of these polymers. in this study, we have investigated the use of an agricultural raw material, sugarcane mo ...	2011	22147642
Epiphytic pink-pigmented methylotrophic bacteria enhance germination and seedling growth of wheat (Triticum aestivum) by producing phytohormone.	Methylotrophic bacteria were isolated from the phyllosphere of different crop plants such as sugarcane, pigeonpea, mustard, potato and radish. The methylotrophic isolates were differentiated based on growth characteristics and colony morphology on methanol supplemented ammonium mineral salts medium. Amplification of the mxaF gene helped in the identification of the methylotrophic isolates as belonging to the genus Methylobacterium. Cell-free culture filtrates of these strains enhanced seed germi ...	2011	22200783
Continuous two stage acetone-butanol-ethanol fermentation with integrated solvent removal using Clostridium acetobutylicum B 5313.	The objective of this study was to optimize continuous acetone-butanol-ethanol (ABE) fermentation using a two stage chemostat system integrated with liquid-liquid extraction of solvents produced in the first stage. This minimized end product inhibition by butanol and subsequently enhanced glucose utilization and solvent production in continuous cultures of Clostridium acetobutylicum B 5313. During continuous two-stage ABE fermentation, sugarcane bagasse was used as the cell holding material for ...	2011	22197332
heterologous expression of manganese peroxidase in aspergillus niger and its effect on phenanthrene removal from soil.	a strain of aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. transformants were selected based on their resistance to hygromycin b and the discoloration induced on poly r-478 dye by the peroxidase activity. the recombinant a. niger sbc2-t3 stra ...	2011	22286039