Publications

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dark and light repair in ultraviolet-irradiated acholeplasma laidlawii. 19725060072
some studies on the fatty acid composition of total lipids and phosphatidylglycerol from acholeplasma laidlawii b and their relation to the premeability of intact cells of this organism. 19725070656
selective solubilization with tween 20 of membrane proteins from acholeplasma laidlawaii. 19725082994
phase transitions of phospholipid bilayers and membranes of acholeplasma laidlawii b visualized by freeze fracturing electron microscopy. 19725082995
the effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact acholeplasma laidlawii cell membranes and derived liposomes. 19725011001
studies on the biological properties of polyene antibiotics. evidence for the direct interaction of filipin with cholesterol. 19725012767
[stimulating effect of langat virus on reproduction of mycoplasma laidlawii in combined infection of a primary culture of chick embryo cells]. 19724672317
improved activity sedimentation method. 19725062881
mycoplasma isolations from clinically normal cats. 19725063126
thermal-turbidimetric studies of membranes from acholeplasma laidlawii. 19725070088
relations between lipid composition and permeability of membranes. 19724680245
[study of mycoplasma isolated from cases of swine pneumonia]. 19725081406
polyacrylamide-gel electrophoresis of cell proteins of mycoplasma isolated from cattle and horses. 19725017869
distribution of mycoplasmas in the non-pneumonic bovine respiratory tract. 19725018036
lipid mobility in acholeplasma membranes using deuteron magnetic resonance. 19725041152
osmolar concentration and fixation of mycoplasmas.broth cultures of acholeplasma laidlawii were fixed with various concentrations of cacodylate-buffered glutaraldehyde. the shape and ultrastructure of the organisms varied with the osmolar concentration of the fixative. when the fixation mixture was hypertonic to the culture medium, ultrathin sections suggested that the cells had shrunk. phosphate buffer, sodium chloride, or sucrose at comparable osmolaities had the same effect as sodium cacodylate. glutaraldehyde itself also contributed to the ...19724555408
13 c nmr studies of lipids in bilayers and membranes. 19725041946
[morphological properties of mycoplasma laidlawii]. 19725042100
plasticity of biological membranes. 19724346307
protein-lipid interactions. 19724592575
recent studies using nuclear magnetic resonance spectroscopy of lipids and biological membranes. 19724580642
eagle's basal medium as a defined medium for mycoplasma studies. 19724571002
thiopeptin, a new feed-additive antibiotic: biological studies and field trials.thiopeptin is a new antibiotic, produced by streptomyces tateyamensis and developed solely for animal use as a feed additive. the antibiotic content in animal tissue and feed was assayed in terms of the antimicrobial activity against mycoplasma laidlawii a. this antibiotic was found to be relatively nontoxic in rats and mice. in chickens, this antibiotic is excreted into feces within 48 hr of administration and is not absorbed in tissue. it is well tolerated in both broilers and swine and is hig ...19724680812
studies on the biological properties of polyene antibiotics: comparison of other polyenes with filipin in their ability to interact specifically with sterol. 19724565643
permeability and topography of membranes. 19724557530
[studies of the biosynthesis of transfer ribonucleic acids]. 19724560651
[mycoplasma infection of cattle]. 19724562052
the effect of aeration on the growth of acholeplasma (mycoplasma) laidlawii a. 19724553303
[comparative characteristics of methods of culturing mycoplasma and l-forms of bacteria and serological reactions used for their identification]. 19724555023
the growth and morphology of acholeplasma (mycoplasma) laidlawii a in different media. 19724505769
proton magnetic resonance studies of whole human erythrocyte membranes. 19724622201
mycoplasma: growth precipitation as a serodiagnostic method.a serological test is described which is designated the growth precipitation test and is based on the release of diffusible compounds from mycoplasma strains. during growth of mycoplasmas on solid substrates, precipitation lines are formed with antibodies from homologous antisera independent of heat-labile serum factors. the application of the test as a serodiagnostic tool was investigated by performing a complete serological cross-test with 10 mycoplasma strains. the test was compared with the ...19724623280
detection of cholesterol in cell membranes by use of bacterial toxins.a method is described for the detection of cholesterol in membranes from erythrocytes, mycoplasmas, and bacterial cells by a ferritin-labeling technique. membranes treated with cereolysin, a bacterial hemolysin which specifically binds to cholesterol, and then treated with ferritin-antitetanolysin, were specifically ferritin-labeled for cholesterol. a similar antigen-antibody system, streptolysin o-ferritin-antistreptolysin, was also used successfully with erythrocyte membranes. there was an une ...19724623312
ultrastructure of mycoplasmatales virus laidlawii i. 19724116131
[active glucose transport in mycoplasma laidlawii]. 19724636164
hydrolysis of alanine oligopeptides by an enzyme located in the membrane of mycoplasma laidlawii. 19724640765
photodynamic killing of acholeplasma laidlawii. 19724643094
a phosphatidyl diglucosyl diglyceride from acholeplasma laidlawii b. 19724643340
[iron oxidation by mycoplasma laidlawii]. 19724643941
survival of acholeplasma laidlawii a and changes in its dna after irradiation with x- and gamma rays. 19724644259
antibiotics from mycoplasma. i. acholeplasma laidlawii b. 19724645949
[size distribution of colony-forming units in a population of mycoplasma laidlawii]. 19724657956
[sensitivity of mycoplasmas to spiramycin]. 19724669219
the structure of a glycerylphosphoryldiglucosyl diglyceride from the lipids of acholeplasma laidlawii strain b.1. the phosphatidylglucose structure proposed previously (smith & henrikson, 1965) for the glucose-containing phospholipid from acholeplasma laidlawii is incorrect. 2. the structure now proposed is 3-(sn-glycerol-3-phosphoryl-6'-[o-alpha-d-glucopyranosyl-(1-->2)-o-alpha-d-glucopyranosyl])- sn-1,2-diglyceride, a new type of bacterial lipid. 3. deacylation of the lipid gave a single water-soluble phosphate ester which could be distinguished on chromatography from synthetic samples of glucosylphosp ...19724345854
fungistatic activity of lucerne saponins and digitonin as related to sterols. 19724676387
lack of interaction between sendai virus and bacterial cells. 19724338794
characterization of the myoplasma membrane proteins. 3. gel filtration and immunological characterization of acholeplasma laidlawii membrane proteins. 19724339322
elimination of mycoplasma contaminants from virus stocks by treatment with nonionic detergents.five nonionic detergents (tweens 20, 40, 60, and 80, and triton wr-1339) were tested for their ability to inactivate four mycoplasma species which are common contaminants of animal cell cultures. tween 20 was found to be the most effective, in that a concentration of 2.5 mg/ml completely inactivated cultures of m. hominis, m. hyorhinis, and acholeplasma laidlawii within 1 hr and a culture of m. orale within 3 hr. the other detergents exhibited various degree of activity against the different myc ...19724341521
[immunodepressive effect of mycoplasma laidlawii on the formation of humoral antibodies in mice]. 19724344075
silicotungstate negative staining of acholeplasma laidlawii membranes and lipids. 19724118305
glutamate dehydrogenase from mycoplasma laidlawii.mycoplasma laidlawii possesses a single glutamate dehydrogenase (gdh) with dual coenzyme specificity [specificity for nicotinamide adenine dinucleotide (h) and nicotinamide adenine dinucleotide phosphate (h)]. a purification procedure is reported which results in an enzyme preparation with a specific activity of 79.5 units/mg and which displays only one significant protein band after gel electrophoresis. this one band was determined, by activity staining, to have all of the gdh nucleotide specif ...19724112954
electron microscope observations on acholeplasma laidlawii viruses. 19724114278
[mycoplasma (acholeplasma laidlawii) from cold blood cell cultures]. 19724145171
[influence of thallium acetate on the growth of acholeplasmataceae, mycoplasmataceae, and some bacteria species]. 19724145172
[morphological differentiation of some strains of mycoplasma gallisepticum against different mycoplasma species on solid medium]. 19724145213
the temperature requirements of growth of some mycoplasmas. 19724146501
mycoplasmatales virus-laidlawii 2, a new virus isolated from acholeplasma laidlawii. 19714106810
phosphatase activity of some mycoplasma species. 19714361836
isolation and identification of porcine mycoplasma in switzerland. 19714946169
[the effects of glucose on mycoplasma]. 19714947989
mycoplasmas in cell cultures from rheumatoid synovial membranes.ten strains of myocoplasmas were recovered from cultures of synovium or cultures inoculated with synovial fragments from rheumatoid arthritis and one from osteo-arthritis. the source of the organisms is not known. patients with rheumatoid arthritis had no complement-fixing antibody and no fluorescent staining antibody against the mycoplasmas isolated and no mycoplasma antigen was detected by immunofluorescence in sections of synovia and in synovial fluids.the strains isolated were of two main se ...19714948122
a membrane-bound lysophospholipase from mycoplasma laidlawii strain b. 19714993729
[ultrastructure and development of colonies of a strain of mycoplasma laidlawii in solid media]. 19715002964
incorporation of penicillinase into reaggregated mycoplasma membranes. 19715003616
characterization of the mycoplasma membrane proteins. ii. solubilization and enzymic activities of acholeplasma laidlawii membrane proteins. 19714334752
[biochemical investigations on 10 strains of mycoplasma laidlawii]. 19715105631
lipid bilayer structure in the membrane of mycoplasma laidlawii. 19715088924
transfer ribonucleic acid from mycoplasma laidlawii a. 19715545013
presence of two dna populations in mycoplasma laidlawii. 19715551322
[fractionation of the population of mycoplasma laidlawii in urografin density gradient]. 19715567637
[effect of mycoplasma-viral infection of a primary culture of chick embryo cells on interferon production induced by langat virus]. 19715167284
peptidase activity in the membranes of mycoplasma laidlawii. 19715117556
structural comparisons of native and reaggregated membranes from mycoplasma laidlawii and erythrocytes by x-ray diffraction and nuclear magnetic resonance techniques. 19715159791
structural comparisons of native and reaggregated membranes from mycoplasma laidawii and erythrocytes using a fluorescence probe. 19715159792
immunogenicity of cytolipin h aggregated with acholeplasma laidlawii membrane proteins. 19715165763
induction of alpha-glucosidase in mycoplasma laidlawii a. 19715283618
kinetics of sodium dodecyl sulfate solubilization of mycoplasma laidlawii plasma membranes.the kinetics of sodium dodecyl sulfate solubilization of aqueous suspensions of mycoplasma laidlawii membranes have been investigated by light scattering in a stopped-flow apparatus. there was evidence of direct interaction between the membranes and sodium dodecyl sulfate micelles above the critical micelle concentration, although of lower order kinetically than with monomeric dodecyl sulfate anions below the critical micelle concentration. the activation energy remained the same in either case, ...19715289357
[etiology and diagnosis of porcine mycoplasmosis. 3. pathological, anatomical and histological studies of experimentally infected suckling pigs]. 19715131929
characterization of the mycoplasma membrane proteins. i. reaggregation of solubilized membrane proteins of acholeplasma laidlawii. 19715141123
the microscopic examination of the apollo-11 and -12 sample and the quest for life forms. 19715142391
[serologic differentiation between acholeplasma laidlawii (mycoplasma laidlawii) and acholeplasma granularum (mycoplasma granularum) strains using agglutination and precipitation]. 19715169114
production and properties of antisera to membrane glycolipids of mycoplasma pneumoniae.the glycolipid haptens of mycoplasma pneumoniae became immunogenic when bound to membrane proteins of acholeplasma laidlawii by reaggregation. this process consisted of the solubilization of lipid-depleted a. laidlawii membranes and m. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate and dialysis of the mixed solutions against 20 mm mg(2+). the antibodies produced in rabbits to the reaggregated glycolipids inhibited the metabolism of m. pneumoniae, fixed complement with m. pneumoniae glyco ...197116557990
phase transitions and bilayer structure of mycoplasma laidlawii b. 197111945568
electron microscopy of solubilized acholeplasma laidlawii membrane proteins reaggregated with mycoplasma pneumoniae glycolipids. 19715579139
the relationship between fatty acid structure and the positional distribution of esterified fatty acids in phosphatidyl glycerol from mycoplasma laidlawii b. 19705417177
ph-dependent changes in density of plasma membranes of growing mycoplasma laidlawii cells. 197011945407
incorporation and elongation of fatty acid isomers by mycoplasma laidlawii a. 19705412665
x-ray diffraction studies of phase transitions in the membrane of mycoplasma laidlawii. 19705413340
immunogenicity of mycoplasma pneumoniae glycolipids: a novel approach to the production of antisera to membrane lipids.the glycolipid haptens of mycoplasma pneumoniae were bound to membrane proteins of acholeplasma laidlawii (formerly mycoplasma laidlawii) by reaggregation. this process consisted of the solubilization of lipid-depleted a. laidlawii membranes and m. pneumoniae glycolipids in 20 mm sodium dodecyl sulfate, and dialysis of the mixed solutions against 20 mm mg(2+). the hybrid reaggregate, collected by centrifugation, was highly immunogenic in rabbits, eliciting the production of a high titer of antib ...19704943173
isolation of a virus infecting a strain of mycoplasma laidlawii. 19705418254
calorimetric detection of a membrane-lipid phase transition in living cells.the membrane lipids in living mycoplasma laidlawii exhibit a phase transition characteristic of that from crystal to liquid crystal within the bilayer conformation. the transition occurs at the same temperature in viable organisms, membranes isolated from the organisms, and isolated membrane lipids. the enthalpy of the transition in the membrane is compared with that of an aqueous suspension of isolated membrane lipids. the result is consistent with presence of an extended lipid bilayer in the n ...19705420540
characterization of the plasma membrane of mycoplasma laidlawii. vii. phase transitions of membrane lipids. 19705473498
native and reformed mycoplasma laidlawii membranes compared by freeze-etching. 19705473500
the effect of alterations in fatty acid composition and cholesterol content on the permeability of mycoplasma laidlawii b cells and derived liposomes. 19705473512
amended nomenclature for strains related to mycoplasma laidlawii. 19705487182
metabolic turnover of the polar lipids of mycoplasma laidlawii strain b.the fatty acyl groups of the membrane polar lipids of mycoplasma laidlawii b were radioactively labeled by growth in the presence of (14)c-palmitic, oleic, or linoleic acids. no loss of radioactivity from any of the polar lipids occurred during incubation of radiolabeled cells in growth medium containing various nonradioactive fatty acids, although growth and lipid biosynthesis continued throughout the incubation period. the metabolism of the glucose and phosphate moieties was also studied in a ...19705411758
cholesterol requirement of mycoplasmas.cholesterol requirement for growth of mycoplasmas was tested in a serum-free medium supplemented with albumin, l-arginine, palmitic acid, and various concentrations of cholesterol dissolved in tween 80. in cases in which tween 80 was shown to inhibit growth, the test medium was supplemented with cholesterol dissolved in ethanol. of the 31 species examined, all but mycoplasma laidlawii, m. granularum, and mycoplasma species strain s-743 exhibited a growth response to cholesterol. no requirement f ...19704911537
acholeplasma axanthum, sp. n.: a new sterol-nonrequiring member of the mycoplasmatales.mycoplasmas recovered from tissue cultures and previously shown to belong to the sterol-nonrequiring group of mycoplasmas have been further characterized. the biological and serological properties of these strains show them to be clearly distinct from acholeplasma laidlawii and a. granularum, two species of sterol-nonrequiring mycoplasmas recently reclassified. it is proposed that the newly described mycoplasmas be designated acholeplasma axanthum, sp. n.19704919991
ultrastructure of mycoplasma laidlawii during culture development.mycoplasma laidlawii b has been studied by phase-contrast and electron microscopy (thin-section and negative staining). exponential-phase cells are filaments with the following structures: surface unit membrane, nuclear material, ribosomes, and intracellular granular region. these cells appear to reproduce by binary fission. in stationary phase, the filamentous cells have numerous constrictions, giving them a beaded appearance. nonviable death-phase cultures contain cellular debris: swollen cell ...19704192565
the synthesis of long-chain fatty acids by a cell-free system from mycoplasma laidlawii a. 19704391501
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