Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| purification and characterization of a dehydrogenase catalyzing conversion of n alpha-benzyloxycarbonyl-l-aminoadipic-delta-semialdehyde to n alpha-benzyloxycarbonyl-l-aminoadipic acid from rhodococcus sp. aiu z-35-1. | the enzyme catalyzing conversion of n alpha-benzyloxycarbonyl-l-aminoadipic-delta-semialdehyde (n alpha-z-l-aasa) to n alpha-benzyloxycarbonyl-l-aminoadipic acid (n alpha-z-l-aaa) in rhodococcus sp. aiu z-35-1 was identified, and its characteristics were revealed. this reaction was catalyzed by a dehydrogenase with a molecular mass of 59 kda. the dehydrogenase exhibited enzyme activity on not only n alpha-z-l-aasa but also n alpha-z-d-aasa and short chain aliphatic aldehydes, but not on aromatic ... | 2007 | 18086440 |
| ocular pathogen or commensal: a pcr-based study of surface bacterial flora in normal and dry eyes. | to compare the bacterial population of the ocular surface of normal and dry eye subjects using conventional culture and 16s rdna pcr. | 2007 | 18055811 |
| construction of a recombinant plasmid as reaction control in routine pcr for detection of contagious equine metritis (cem-pcr). | contagious equine metritis (cem) is a highly contagious bacterial venereal disease of horses caused by taylorella equigenitalis. cem-pcr is a semi-nested pcr method for detecting this bacterium. although this technique is regarded as a sensitive diagnostic method for cem, there are risks of it generating false positive and false negative results. in this study, we constructed a recombinant plasmid (cem-pos) as reaction control to assure adequate pcr reaction and prevent false positive results ca ... | 2007 | 18057840 |
| [study on cooperating degradation of cypermethrin and 3-phenoxybenzoic acid by two bacteria strains]. | the microbial cooperated reaction is one of the most important forms of microbial degradation of organic pollutants. although there were many research reports of cooperating degradation, less report on the microbial cooperated of pyrethroid degradation to be found. we have isolated one degrading-bacteria strain named cdt3 for degradation of cypermethrin, which can degraded the cypermethrin into 3-pba and dcva. at the same time, we also isolated another degrading-bacteria strain named as pbm11, w ... | 2007 | 18062258 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| characterization and functional analysis of a novel gene cluster involved in biphenyl degradation in rhodococcus sp. strain r04. | isolation of the genes relative to pcb biodegradation and identification of the bph gene function in rhodococcus sp. r04. | 2007 | 18045404 |
| rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium. | a gram-positive, aerobic, non-motile, mesophilic strain, djl-6(t), able to degrade carbendazim, was isolated from a carbendazim-contaminated soil sample from jiangsu province, china. the taxonomic position of this isolate was analysed by using a polyphasic approach. chemotaxonomic analysis including peptidoglycan type, diagnostic sugar composition, fatty acid profile, menaquinones, polar lipids and mycolic acids showed that the characteristics of strain djl-6(t) were in good agreement with those ... | 2007 | 18048720 |
| biosynthesis of bile acids in a variety of marine bacterial taxa. | several marine bacterial strains, which were isolated from seawater off the island dokdo, korea, were screened to find new bioactive compounds such as antibiotics. among them, donghaeana dokdonensis strain dsw-6 was found to produce antibacterial agents, and the agents were then purified and analyzed by lc-ms/ms and 1d- and 2d-nmr spectrometries. the bioactive compounds were successfully identified as cholic acid and glycine-conjugated glycocholic acid, the 7alpha-dehydroxylated derivatives (deo ... | 2007 | 18050942 |
| rhodococcus equi bacteremia with lung abscess misdiagnosed as corynebacterium: a report of 2 cases. | 2007 | 18209924 | |
| rhodococcus equi infection after alemtuzumab therapy for t-cell prolymphocytic leukemia. | rhodococcus equi, mainly known from veterinary medicine as a pathogen in domestic animals, can also cause infections in immunocompromised humans, especially in those with defects in cellular immunity. alemtuzumab, an anti-cd52 monoclonal antibody, causes lymphocytopenia by eliminating cd52-positive cells. we report a patient in whom rhodococcus equi infection developed after alemtuzumab therapy. | 2007 | 18258054 |
| in vitro immunomodulating activity of biosurfactant glycolipid complex from rhodococcus ruber. | the biosurfactant glycolipid complex synthesized by rhodococcus ruber actinobacteria is not toxic and exhibits no appreciable effect on proliferative activity of peripheral blood leukocytes. in the monocyte fraction, the biosurfactant activates the production of il-1beta and tnf-alpha cytokines without modifying the production of il-6. in the mononuclear fraction, the glycolipid biosurfactant exhibited no effects on the production of il-1beta, tnf-alpha, and il-6. these results indicate good pro ... | 2007 | 18457028 |
| resistance studies of erythromycin and rifampin for rhodococcus equi over a 10-year period. | this study sought to determine whether an increase in resistance of rhodococcus equi to the antibiotics rifampin and erythromycin occurred over a 10-year period. this was carried out by the use of e test strips for rifampin and erythromycin to determine the mic (minimum inhibitory concentration) values of rhodococcus equi to this combination of antibiotics.the findings of this study indicated that there was an increase in resistance of rhodococcus equi to rifampin and erythromycin over the 10-ye ... | 2007 | 21851690 |
| the tobacco ntann12 gene, encoding an annexin, is induced upon rhodoccocus fascians infection and during leafy gall development. | summary annexins are calcium-binding proteins that have been associated in plants with different biological processes such as responses to abiotic stress and early nodulation stages. until now, the implication of annexins during plant-pathogen interactions has not been reported. here, a novel plant annexin gene induced in tobacco by-2 cell suspension cultures infected with the phytopathogenic bacterium rhodococcus fascians (strain d188) has been identified. expression of this gene, called ntann1 ... | 2007 | 20507490 |
| the phytopathogen rhodococcus fascians breaks apical dominance and activates axillary meristems by inducing plant genes involved in hormone metabolism. | summary rhodococcus fascians is a gram-positive bacterium that interacts with many plant species and induces multiple shoots through a combination of activation of dormant axillary meristems and de novo meristem formation. although phenotypic analysis of the symptoms of infected plants clearly demonstrates a disturbance of the phytohormonal balance and an activation of the cell cycle, the actual mechanism of symptom development and the targets of the bacterial signals are unknown. to elucidate t ... | 2006 | 20507431 |
| biosurfactant production by rhodococcus erythropolis grown on glycerol as sole carbon source. | the production of biosurfactant by rhodococcus erythropolis during the growth on glycerol was investigated. the process was carried out at 28 degrees c in a 1.5-l bioreactor using glycerol as carbon source. the bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. after 51 h of cultivation, 1.7 g/l of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mn/m, respectively, 67% of emulsifying index (e (24)), and ... | 2006 | 18563662 |
| fermentation reactor coupled with capillary electrophoresis for on-line bioprocess monitoring. | in this paper, a filter probe integrated into a computerized pneumatic sampler for capillary electroseparations was developed for an on-line monitoring of bioprocesses. the optimization of the performance of the coupled system was done by using a response surface modeling and three-level two-factor design. the resolution was found to be the most important parameter influencing the performance of an on-line analysis of the microbial metabolism. for the on-line analysis the migration time and dete ... | 2006 | 16495032 |
| transformation of the herbicide 2,6-dichlorobenzonitrile to the persistent metabolite 2,6-dichlorobenzamide (bam) by soil bacteria known to harbour nitrile hydratase or nitrilase. | in soil the herbicide 2,6-dichlorobenzonitrile (dichlobenil) is degraded to the persistent metabolite 2,6-dichlorobenzamide (bam) which has been detected in 19% of samples taken from danish groundwater. we tested if common soil bacteria harbouring nitrile-degrading enzymes, nitrile hydratases or nitrilases, were able to degrade dichlobenil in vitro. we showed that several strains degraded dichlobenil stoichiometrically to bam in 1.5-6.0 days; formation of the amide intermediate thus showed nitri ... | 2006 | 16496093 |
| microbial succession in a compost-packed biofilter treating benzene-contaminated air. | air artificially contaminated with increasing concentrations of benzene was treated in a laboratory scale compost-packed biofilter for 240 days with a removal efficiency of 81-100%. the bacterial community in the packing material (pm) at different heights of the biofilter was analysed every 60 days. bacterial plate counts and ribosomal intergenic spacer analysis (risa) of the isolated strains showed that the number of cultivable aerobic heterotrophic bacteria and the species diversity increased ... | 2006 | 16502043 |
| biological degradation of aflatoxin b1 by rhodococcus erythropolis cultures. | aflatoxin contamination of food and grain poses a serious economic and health problem worldwide, but particularly in africa. aflatoxin b(1) (afb(1)) is extremely mutagenic, toxic and a potent carcinogen to both humans and livestock and chronic exposure to low levels of afb(1) is a concern. in this study, the biodegradation of aflatoxin b(1) (afb(1)) by rhodococcus erythropolis was examined in liquid cultures using thin layer chromatography (tlc), high performance liquid chromatography (hplc), el ... | 2006 | 16504326 |
| crystallization and preliminary x-ray analysis of a bacterial l-amino-acid oxidase from rhodococcus opacus. | l-amino-acid oxidases (ec 1.4.3.2) catalyse the stereospecific oxidative deamination of an l-amino-acid substrate to an alpha-keto acid with the production of ammonia and hydrogen peroxide. in this study, the crystallization and preliminary x-ray analysis of a bacterial l-amino-acid oxidase from rhodococcus opacus (rolaao) is described. rolaao is a dimeric protein consisting of two identical subunits of 489 amino acids with a calculated molecular weight of 54.2 kda and a non-covalently bound fad ... | 2006 | 16511322 |
| bioremediation of hydrocarbon-contaminated polar soils. | bioremediation is increasingly viewed as an appropriate remediation technology for hydrocarbon-contaminated polar soils. as for all soils, the successful application of bioremediation depends on appropriate biodegradative microbes and environmental conditions in situ. laboratory studies have confirmed that hydrocarbon-degrading bacteria typically assigned to the genera rhodococcus, sphingomonas or pseudomonas are present in contaminated polar soils. however, as indicated by the persistence of sp ... | 2006 | 16514512 |
| antimicrobial activity of gallium against virulent rhodococcus equiin vitro and in vivo. | rhodococcus equi, a facultative intracellular bacterium, causes severe pneumonia in foals. evidence suggests that most foals become infected very early in life, when they have immature or ineffective innate immune responses. this study evaluated the antimicrobial activity of gallium against r. equi, as a potential chemoprophylactic and therapeutic agent. rhodococcus equi was grown in media with various concentrations of gallium nitrate (gn), with and without excess iron. gn significantly inhibit ... | 2006 | 16515666 |
| selective biodegradation of s and n heterocycles by a recombinant rhodococcus erythropolis strain containing carbazole dioxygenase. | the carbazole dioxygenase genes were introduced into a dibenzothiophene degrader. the recombinant rhodococcus erythropolis sn8 was capable of efficiently degrading dibenzothiophene and carbazole simultaneously. sn8 could also degrade various alkylated derivatives of carbazole and dibenzothiophene in fs4800 crude oil by just a one-step bioprocess. | 2006 | 16517679 |
| biofilm development of the polyethylene-degrading bacterium rhodococcus ruber. | we have recently isolated a biofilm-producing strain (c208) of rhodococcus ruber that degraded polyethylene at a rate of 0.86% per week (r2=0.98). strain c208 adheres to polyethylene immediately upon exposure to the polyolefin. this initial biofilm differentiates (in a stepwise process that lasts about 20 h) into cell-aggregation-forming microcolonies. further organization yields "mushroom-like" three-dimensional structures on the mature biofilm. the ratio between the population densities of the ... | 2006 | 16534612 |
| convenient treatment of acetonitrile-containing wastes using the tandem combination of nitrile hydratase and amidase-producing microorganisms. | this study aimed to construct an acetonitrile-containing waste treatment process by using nitrile-degrading microorganisms. to degrade high concentrations of acetonitrile, the microorganisms were newly acquired from soil and water samples. although no nitrilase-producing microorganisms were found to be capable of degrading high concentrations of acetonitrile, the resting cells of rhodococcus pyridinivorans s85-2 containing nitrile hydratase could degrade acetonitrile at concentrations as high as ... | 2006 | 16402166 |
| spectroscopic and substrate binding properties of heme-containing aldoxime dehydratases, oxdb and oxdre. | aldoxime dehydratase (oxd) is a novel hemeprotein that catalyzes the dehydration reaction of aldoxime to produce nitrile. in this study, we studied the spectroscopic and substrate binding properties of two oxds, oxdb from bacillus sp. strain oxb-1 and oxdre from rhodococcus sp. n-771, that show different quaternary structures and relatively low amino acid sequence identity. electronic absorption and resonance raman spectroscopy revealed that ferric oxdre contained a six-coordinate low-spin heme, ... | 2006 | 16414119 |
| preferential attack of the (s)-configured ether-linked carbons in bis-(1-chloro-2-propyl) ether by rhodococcus sp. strain dtb. | rhodococcus sp. strain dtb (dsm 44534) was grown on a mixture of (r,r)-, (s,s)- and meso-bis-(1-chloro-2-propyl) ether (bcpe) as the sole source of carbon and energy. during bcpe degradation 1'-chloro-2'-propyl-3-chloro-2-prop-1-enyl-ether (dve), 1-chloro-2-propanol and chloroacetone intermediates were formed. the bcpe or dve stereoisomers were metabolized in consecutive order via scission of the ether bond, with discrimination against the (r) configuration. resting cell suspensions of rhodococc ... | 2006 | 16420620 |
| study of a hexane-degrading consortium in a biofilter and in liquid culture: biodiversity, kinetics and characterization of degrading strains. | a gasoline-degrading consortium, originating from a mexican soil, was used to study its hexane-degradation kinetics in liquid culture and in a biofilter with mineral support. the biodiversity of the consortium depending on the culture conditions and electron and energy source (gasoline, hexane in liquid or hexane in the biofilter) was analyzed using a 16s rrna-based approach. significant differences between the populations were observed, indicating a probable adaptation to the substrate. two str ... | 2006 | 16420632 |
| potential of rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol. | the potential of two rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol was investigated. genome sequence data of rhodococcus sp. i24 suggested a coenzyme a-dependent, non-beta-oxidative pathway for ferulic acid bioconversion, which involves feruloyl-coa synthetase (fcs), enoyl-coa hydratase/aldolase (ech), and vanillin dehydrogenase (vdh). this pathway was proven for rhodococcus opacus pd630 by physiological characterization of knockout mutants. however, ... | 2006 | 16421716 |
| sequence analysis of three plasmids harboured in rhodococcus erythropolis strain pr4. | rhodococcus erythropolis strain pr4 has been isolated as an alkane-degrading bacterium. the strain harbours one linear plasmid, prel1 (271 577 bp) and two circular plasmids, prec1 (104 014 bp) and prec2 (3637 bp), all with some sequence similarities to other rhodococcus plasmids. for prel1, prec1 and prec2, 298, 102 and 3 open reading frames, respectively, were predicted. linear plasmid prel1 has several regions homologous to plasmid pbd2 found in r. erythropolis bd2. sequence analysis of prel1 ... | 2006 | 16423019 |
| recurrent rhodococcus equi infection with fatal outcome in an immunocompetent patient. | the majority of human rhodococcus equi infections occur in immunocompromised hosts, especially those with aids, and infection in immunocompetent patients is rare. reported here is a case of r. equi infection in a seemingly healthy patient with a very complicated course. despite neurosurgery and prolonged antibiotic therapy the patient deceased. | 2006 | 16424975 |
| intracranial abscess caused by rhodococcus equi infection in a foal. | a 4-month-old missouri fox trotter colt was examined for a 5-week history of head tilt after treatment for suspected pulmonary rhodococcus equi infection. | 2006 | 16426201 |
| enhanced biodegradation of diesel oil by a newly identified rhodococcus baikonurensis en3 in the presence of mycolic acid. | the aim of the present study was to isolate and characterize a bacterium, strain en3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic mycolic acid (2-hexyl-3-hydroxyldecanoic acid). | 2006 | 16430509 |
| resolution of methyl nonactate by rhodococcus erythropolis under aerobic and anaerobic conditions. | [reaction: see text]. an efficient resolution of methyl nonactate is reported by biotransformation in shake flask cultures of rhodococcus erythropolis. the equilibrium of the reaction redox system can be manipulated by switching from aerobic to anaerobic growth, thereby generating both enantiomers of the target in excellent yield and enantiomeric purity. | 2006 | 16435855 |
| structural analysis of an extracellular polysaccharide produced by a benzene tolerant bacterium, rhodococcus sp. 33. | rhodococcus sp. 33 can tolerate and efficiently degrade various concentrations of benzene, one of the most toxic and prevailing environmental pollutants. this strain produces a large quantity of extracellular polysaccharide (33 eps), which plays an important role in the benzene tolerance in rhodococcus sp. 33, especially by helping the cells to survive an initial challenge with benzene. this eps has been reported to be composed of d-galactose, d-glucose, d-mannose, d-glucuronic acid, and pyruvic ... | 2006 | 16442512 |
| antibacterial activity of cefquinome against equine bacterial pathogens. | cefquinome is known for its use as an antibacterial drug in cattle and pigs. the objective of this study was to evaluate the antibacterial activity of cefquinome against equine pathogenic bacteria. the minimum inhibitory concentration (mic) of cefquinome was determined for a total of 205 strains, which had recently been isolated in europe from diseased horses (respiratory infection, foal septicaemia). the bactericidal activity was tested against 19 strains using the time killing method. the post ... | 2006 | 16455213 |
| molecular cloning and expression of genes encoding a novel dioxygenase involved in low- and high-molecular-weight polycyclic aromatic hydrocarbon degradation in mycobacterium vanbaalenii pyr-1. | mycobacterium vanbaalenii pyr-1 is able to metabolize a wide range of low- and high-molecular-weight (hmw) polycyclic aromatic hydrocarbons (pahs). a 20-kda protein was upregulated in pah-metabolizing m. vanbaalenii pyr-1 cells compared to control cultures. the differentially expressed protein was identified as a beta subunit of the terminal dioxygenase using mass spectrometry. pcr with degenerate primers designed based on de novo sequenced peptides and a series of plaque hybridizations were don ... | 2006 | 16461648 |
| mycobacterium tuberculosis prcba genes encode a gated proteasome with broad oligopeptide specificity. | genes predicted to be associated with the putative proteasome of mycobacterium tuberculosis (mtb) play a critical role in defence of the bacillus against nitrosative stress. however, proteasomes are uncommon in eubacteria and it remains to be established whether mtb's prcba genes in fact encode a proteasome. we found that coexpression of recombinant prcb and prca in escherichia coli over a prolonged period at 37 degrees c allowed formation of an alpha(7)beta(7)beta(7)alpha(7), 750 kda cylindrica ... | 2006 | 16468985 |
| j1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from bacillus laterosporus j1, is a member of the alpha/beta-hydrolase fold superfamily. | j1 acylase, a glutaryl-7-aminocephalosporanic acid acylase (gca) isolated from bacillus laterosporus j1, has been conventionally grouped as the only member of class v gca, although its amino acid sequence shares less than 10% identity with members of other classes of gca. instead, it shows higher sequence similarities with rhodococcus sp. strain mb1 cocaine esterase (rhcoce) and acetobacter turbidans alpha-amino acid ester hydrolase (ataeh), members of the alpha/beta-hydrolase fold superfamily. ... | 2006 | 16469317 |
| growth rate and nutrient limitation affect the transport of rhodococcus sp. strain dn22 through sand. | rhodococcus strain dn22 grows on the nitramine explosive rdx as a sole nitrogen source, and is potentially useful for bioremediation of explosives-contaminated soil. in order for strain dn22 to be effectively applied in situ, inoculum cells must reach zones of rdx contamination via passive transport, a process that is difficult to predict at field-scale. we examined the effect of growth conditions on the transport of dn22 cells through sand columns, using chemostat-grown cultures. strain dn22 fo ... | 2006 | 16477351 |
| two cases of equine abortion caused by rhodococcus equi. | rhodococcus equi was isolated from lung, liver, spleen, and stomach content of two aborted equine fetuses of 7 and 8 months gestation from two different farms. lesions included diffuse pyogranulomatous pneumonia with numerous gram-positive coccobacilli within the cytoplasm of macrophages, multinucleated langhans giant cells and neutrophils, and enhanced extramedullary hematopoiesis with megakaryocytosis within the liver and spleen. detection of r. equi was made by bacteriology and immunohistoche ... | 2006 | 16537942 |
| endobacteria in some ectomycorrhiza of scots pine (pinus sylvestris). | the diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes (suillus flavidus, suillus variegatus, russula paludosa and russula sp.) of scots pine (pinus sylvestris) was analysed by restriction fragment length polymorphism profiling of pcr-amplified rdna intergenic spacer regions and by sequence analyses of 16s rrna genes. ectomycorrhizal root tip surface-sterilization methods were developed and assessed for their efficiencies. bacterial communities from s ... | 2006 | 16542403 |
| the degradation of n-hexadecane in soil by thermophilic geobacilli. | soil microcosms have been used to demonstrate the ability of indigenous soil thermophiles to degrade effectively a representative alkane (n-hexadecane). a fragment of the alkane mono-oxygenase gene (alkb) was amplified from thermophilic geobacillus thermoleovorans strain t70 by pcr using degenerate primers. the amplicon demonstrated 96% sequence similarity with the alkb gene from rhodococcus erythropolis. critical controls ensured that the positive pcr signal detected was not a result of mesophi ... | 2006 | 16542404 |
| proteomic and transcriptional characterization of aromatic degradation pathways in rhodoccocus sp. strain tfb. | rhodococcus sp. strain tfb is a versatile gram-positive bacterium able to grow on a wide variety of aromatic compounds as carbon and energy sources. since the strain is refractory to genetic analysis, a proteomic approach was used to study the metabolic pathways involved in the catabolism of such compounds by analyzing differentially induced proteins. the most marked difference was observed when the proteome profiles of phthalate-grown cells were compared with those cultured in the presence of t ... | 2006 | 16544280 |
| biocatalytic approaches for the quantitative production of single stereoisomers from racemates. | strategies for the chemoenzymatic transformation of a racemate into a single stereoisomeric product in quantitative yield have been developed. a range of industrially relevant alpha-hydroxycarboxylic acids was deracemized in a stepwise fashion via lipase-catalysed enantioselective o-acylation, followed by mandelate racemase-catalysed racemization of the remaining non-reacted substrate enantiomer. alternatively, aliphatic alpha-hydroxycarboxylic acids were enzymatically isomerized using whole res ... | 2006 | 16545098 |
| rhodococcus and mycobacterium tuberculosis: masquerade or mixed infection. | rhodocci have a morphology similar to that of mycobacterium tuberculosis (tb), and are indistinguishable from normal diphtheroid flora. symptoms include fever, productive/non-productive cough and pleuritic chest pain. rhodococcal infections, being resistant to routine anti-tuberculosis medications, may be misdiagnosed as drug-resistant tb, thus prompting treatment for tb with rifampicin-containing regimens that promote the emergence of resistance. we present here a sputum smear afb-positive case ... | 2006 | 16562721 |
| a nucleotide mutation associated with fluoroquinolone resistance observed in gyra of in vitro obtained rhodococcus equi mutants. | in this study, the quinolone resistance-determining region (qrdr) in gyra and gyrb of in vitro fluoroquinolone-resistant rhodococcus equi mutants was sequenced. these mutants were selected from four r. equi strains on blood agar plates containing ciprofloxacin or enrofloxacin. each mutant became 8- to 64 or greater-fold resistant to fluoroquinolones compared with their parent strains. from the results of sequence analysis of qrdr in gyra and gyrb, a nucleotide mutation of codon gac for ggc in gy ... | 2006 | 16563665 |
| [the properties of hydrocarbon-oxidizing bacteria isolated from the oilfields of tatarstan, western siberia, and vietnam]. | eleven strains of hydrocarbon-oxidizing bacteria, isolated from oilfields, representing the genera rhodococcus, gordonia, dietzia, and pseudomonas, were characterized as mesophiles and neutrophiles. rhodococci were halotolerant microorganisms growing in a media containing up to 15% nacl. all the strains oxidized n-alkanes of crude oil. an influence of the cultivation temperatures (28 or 45 degrees c) and organic supplements on the degradation of c12-c30 n-alkanes in oxidized oil by two bacterial ... | 2006 | 16579448 |
| pet ownership in immunocompromised children--a review of the literature and survey of existing guidelines. | pet ownership has been associated with both emotional and physical health benefits. however, owning pets may also pose health risks to immunocompromised patients through zoonotic transmission of disease. our initial impression was that there is a lack of any evidence base in information given by health care professionals regarding these risks. we therefore aimed to produce evidence-based guidelines addressing this issue. a pubmed search was undertaken and a variety of literature on zoonoses revi ... | 2006 | 16581294 |
| polychlorinated biphenyl (pcb)-degrading bacteria associated with trees in a pcb-contaminated site. | the abundance, identities, and degradation abilities of indigenous polychlorinated biphenyl (pcb)-degrading bacteria associated with five species of mature trees growing naturally in a contaminated site were investigated to identify plants that enhance the microbial pcb degradation potential in soil. culturable pcb degraders were associated with every plant species examined in both the rhizosphere and root zone, which was defined as the bulk soil in which the plant was rooted. significantly high ... | 2006 | 16597927 |
| discovery of natural atypical nonhemolytic listeria seeligeri isolates. | we found seven listeria isolates, initially identified as isolates with the xyl(+) rha(-) biotype of listeria welshimeri by phenotypic tests, which exhibited discrepant genotypic properties in a well-validated listeria species identification oligonucleotide microarray. the microarray gives results of these seven isolates being atypical hly-negative l. seeligeri isolates, not l. welshimeri isolates. the aberrant l. seeligeri isolates were d-xylose fermentation positive, l-rhamnose fermentation ne ... | 2006 | 16597942 |
| degradation of aroclor 1242 dechlorination products in sediments by burkholderia xenovorans lb400(ohb) and rhodococcus sp. strain rha1(fcb). | burkholderia xenovorans strain lb400, which possesses the biphenyl pathway, was engineered to contain the oxygenolytic ortho dehalogenation (ohb) operon, allowing it to grow on 2-chlorobenzoate and to completely mineralize 2-chlorobiphenyl. a two-stage anaerobic/aerobic biotreatment process for aroclor 1242-contaminated sediment was simulated, and the degradation activities and genetic stabilities of lb400(ohb) and the previously constructed strain rha1(fcb), capable of growth on 4-chlorobenzoat ... | 2006 | 16597946 |
| molecular typing of vapa-positive rhodococcus equi isolates from jeju native horses, korea. | we recently demonstrated the presence of virulence-associated protein antigen (vapa)-positive rhodococcus equi in jeju island, korea. these bacteria contained one of two distinct plasmid types, a 90-kb type ii plasmid, which has been found in isolates from the native kiso horses of japan, and a new variant, a 90-kb type v plasmid. however, the genotypic characters of the vapa-positive r. equi from jeju native horses and their environments are poorly understood. ninety-eight isolates from soil sa ... | 2006 | 16598168 |
| cloning, expression, and characterization of a self-sufficient cytochrome p450 monooxygenase from rhodococcus ruber dsm 44319. | a new member of class iv of cytochrome p450 monooxygenases was identified in rhodococcus ruber strain dsm 44319. as the genome of r. ruber has not been sequenced, a p450-like gene fragment was amplified using degenerated primers. the flanking regions of the p450-like dna fragment were identified by directional genome walking using polymerase chain reaction. the primary protein structure suggests a natural self-sufficient fusion protein consisting of ferredoxin, flavin-containing reductase, and p ... | 2006 | 16607529 |
| plasmid profiles of virulent rhodococcus equi isolates from soil environment on horse-breeding farms in hungary. | the plasmid profiles of virulent rhodococcus equi strains isolated on three horse-breeding farms located in different parts of hungary were investigated. from 49 soil samples collected on the three farms, 490 r. equi isolates (10 from each sample) were obtained and tested for the presence of 15- to 17-kda antigens (vapa) by immunoblotting and pcr. ninety-eight vapa-positive isolates were detected from 30 of the 49 culture-positive samples with a prevalence ranging from 13.1% to 23.2%. of the 98 ... | 2006 | 16613022 |
| foals are interferon gamma-deficient at birth. | the increased vulnerability of foals to specific pathogens such as rhodococcus equi is believed to reflect an innate immunodeficiency, the nature of which remains poorly understood. previous studies have demonstrated that neonates of many species fail to mount potent th1 responses. the current research investigates the ability of circulating and pulmonary lymphocytes of developing foals to produce interferon gamma (ifngamma). peripheral blood mononuclear cells (pbmc) were prepared from up to 10 ... | 2006 | 16621024 |
| molecular analysis of microbial communities identified in different developmental stages of ixodes scapularis ticks from westchester and dutchess counties, new york. | ixodes scapularis ticks play an important role in the transmission of a wide variety of pathogens between various mammalian species, including humans. pathogens transmitted by ticks include borrelia, anaplasma and babesia. although ticks may harbour both pathogenic and non-pathogenic microflora, little is known about how the diversity of the microflora within ticks may influence the transmission of pathogens. to begin addressing this question, we examined the composition of bacterial communities ... | 2006 | 16623735 |
| methods for the preparation of a biodesulfurization biocatalyst using rhodococcus sp. | several methods to prepare a biodesulfurization (bds) biocatalyst were investigated in this study using a strain of rhodococcus sp. 1awq. this bacterium could selectively remove sulfur from dibenzothiophene (dbt) via the "4s" pathway. dbt, dimethylsulfoxide (dmso), sodium sulphate and mixed sulfur sources were used to study their influence on cell density, desulfurization activity, desulfurization ability, and the cost of biocatalyst production. in contrast to that observed from bacteria culture ... | 2006 | 16624377 |
| rhodococcus equi pneumonia in a foal. | 2006 | 16627120 | |
| rhodococcus kroppenstedtii sp. nov., a novel actinobacterium isolated from a cold desert of the himalayas, india. | the taxonomic position of an actinomycete, strain k07-23t, isolated from a cold desert of the himalayas, india, was established by a polyphasic approach. the strain exhibited phenotypic characters that were typical of the genus rhodococcus. 16s rrna gene sequence (1467 bases) comparisons confirmed that strain k07-23t belongs to the genus rhodococcus. 16s rrna sequence similarity studies showed that the isolate is very closely related to nocardia corynebacterioides dsm 20151t (98.6 %), which has ... | 2006 | 16627641 |
| clinical evaluation of a peptide-elisa based upon n-terminal b-cell epitope of the vapa protein for diagnosis of rhodococcus equi pneumonia in foals. | a total of 227 field samples from naturally exposed foals aged between 3 weeks and 6 months were used in an evaluation of a peptide-based enzyme-linked immunosorbent assay (elisa) for diagnosis of rhodococcus equi infection. a biotinylated peptide derived from the virulence-associated protein a (vapa) of r. equi, a horse pathogen, was synthesized and designated as pn11-14. the peptide corresponds to the n-terminal b-cell epitope tslnlqkdepngrasdtagq of the vapa protein. based upon a serum immuno ... | 2006 | 16629724 |
| evidence for the role of the monb genes in polyether ring formation during monensin biosynthesis. | ionophoric polyethers are produced by the exquisitely stereoselective oxidative cyclization of a linear polyketide, probably via a triepoxide intermediate. we report here that deletion of either or both of the monbi and monbii genes from the monensin biosynthetic gene cluster gave strains that produced, in place of monensins a and b, a mixture of c-3-demethylmonensins and a number of minor components, including c-9-epi-monensin a. all the minor components were efficiently converted into monensin ... | 2006 | 16632258 |
| mutational study on alphagln90 of fe-type nitrile hydratase from rhodococcus sp. n771. | nitrile hydratase (nhase) from rhodococcus sp. n771 is a non-heme iron enzyme having post-translationally modified cysteine ligands, alphacys112-so2h and alphacys114-soh. we replaced alphagln90, which is conserved in all known nhases and involved in the hydrogen-bond network around the catalytic center, with glutamic acid or asparagine. the kcat of alphaq90e and alphaq90n mutants decreased to 24% and 5% that of wild type respectively, but the effect of mutations on km was not very significant. i ... | 2006 | 16636455 |
| microbial desulfurization of gasoline by free whole-cells of rhodococcus erythropolis xp. | rhodococcus erythropolis xp could grow well with condensed thiophenes, mono-thiophenic compounds and mercaptans present in gasoline. rhodococcus erythropolis xp was also capable of efficiently degrading the condensed thiophenes in resting cell as well as biphasic reactions in which n-octane served as a model oil phase. free whole cells of r. erythropolis xp were adopted to desulfurize fluid catalytic cracking (fcc) and straight-run (sr) gasoline oils. about 30% of the sulfur content of fcc gasol ... | 2006 | 16640586 |
| structural analysis of an extracellular polysaccharide produced by rhodococcus rhodochrous strain s-2. | a possibility has been suggested of applying the eps produced by rhodococcus rhodochrous strain s-2 (s-2 eps) to the bioremediation of oil-contaminated environments, because its addition, together with minerals, to oil-contaminated seawater resulted in emulsification of the oil, increased the degradation of polyaromatic hydrocarbons (pah) of the oil, and led to the dominance of pah-degrading marine bacteria. to understand the underlying principles of these phenomena, we determined the chemical s ... | 2006 | 16676419 |
| comparison of concentrations of rhodococcus equi and virulent r. equi in air of stables and paddocks on horse breeding farms in a temperate climate. | rhodococcoccus equi is a significant cause of bronchopneumonia in foals worldwide. infection of the lungs is believed to result from inhalation of virulent r. equi in dust from contaminated environments. a measure of infectious risk in an environment is the level of airborne contamination. | 2006 | 16706283 |
| the impact of bioaugmentation on metal cyanide degradation and soil bacteria community structure. | metal cyanides are significant contaminants of many soils found at the site of former industrial activity. in this study we isolated bacteria capable of degrading ferric ferrocyanide and k2ni(cn)4. one of these bacteria a rhodococcus spp. was subsequently used to bioaugment a minimal medium broth, spiked with k2ni(cn)4, containing 1 g of either an uncontaminated topsoil or a former coke works site soil. degradation of the k2ni(cn)4 was observed in both soils, however, bioaugmentation did not sig ... | 2006 | 16715400 |
| [rhodococcus equi]. | 2006 | 16721450 | |
| enzymatic c-4 deacetylation of 10-deacetylbaccatin iii. | second-generation paclitaxel analogues that require replacement of the c-4 acetate by other substituents are in development. an enzyme able to specifically remove the c-4 acetate from paclitaxel could simplify preparation of the analogues. several strains were isolated from soil samples that contain enzyme activities able to 4-deacetylate 10-dab (10-deacetylbaccatin iii). selection was made using plates containing 10-dab as the sole carbon source and screening colonies for deacetylation of 10-da ... | 2006 | 16722823 |
| bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability. | five bacterial strains were compared for halogenated compounds conversion in aqueous media. depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees c, while optimal ph raised from 8.4 to 9.0. the most effective dehalogenase activity for 1-chlorobutane conversion was detected with rhodococcus erythropolis ncimb13064 and escherichia coli bl21 (de3) (dhaa). the presence of 2-chlorobutane or propanal in the aqueous media could inhibit ... | 2006 | 16723151 |
| characterization of rhodococcus-e. coli shuttle vector pnc9501 constructed from the cryptic plasmid of a propene-degrading bacterium. | rhodococcus-e. coli shuttle vector pnc9501 was constructed using circular cryptic plasmid pnc903 from propene-degrading rhodococcus ruber p-ii-123-1. sequence analysis of pnc903 revealed two open-reading frames encoding the replication proteins reps a and b. in the amino acid sequence of the putative rep b, a helix-turn-helix motif, which is responsible for the binding of dna, was found. sequencing of the upstream region of the putative rep a and incompatibility tests revealed that pnc903 is a m ... | 2006 | 16732453 |
| biocatalytic deuterium- and hydrogen-transfer using over-expressed adh-'a': enhanced stereoselectivity and 2h-labeled chiral alcohols. | employing the over-expressed highly organic solvent tolerant alcohol dehydrogenase adh-'a' from rhodococcus ruber dsm 44541, versatile building blocks, which were not accessible by the wild type catalyst, were obtained in > 99% e.e.; furthermore, employing d8-2-propanol as deuterium source, stereoselective biocatalytic deuterium transfer was made feasible to furnish enantiopure deuterium labeled sec-alcohols on a preparative scale employing a single enzyme. | 2006 | 16733594 |
| genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from rhodococcus opacus strain sao101. | rhodococcus opacus strain sao101 was shown to degrade on various polycyclic aromatic hydrocarbons such as naphthalene, dibenzofuran (df), and dibenzo-p-dioxin (dd). one of the unique traits of the strain sao101 is its ability to oxidize df compounds by lateral dioxygenation. to clone the lateral dioxygenase gene involved in compound degradation in strain sao101, we identified a cosmid clone that oxidizes aromatic compounds by using sao101 genomic dna. sequencing analysis revealed that isolated c ... | 2006 | 16736088 |
| [co-expression of rhodococcus sp. ds-3 dszabc and dszd gene with incompatible plasmids in escherichia coli]. | biological dusulfurizaion of petroleum feedstocks and products may offer an attractive alternative to reduce sulfur oxide emissions that cause serious environmental pollution. dibenzothiophene (dbt), a model of organic sulfur compound in petroleum, can be microbially desulfurized without degradation of the organic structure by 4s pathway. three desulfurization enzymes (dsza, dszb and dszc) and flavin reductase (dszd) are involved in sulfur-specific dbt desulfurization. dsza and dszc are fmnh2-de ... | 2006 | 16736591 |
| proteome changes after metabolic engineering to enhance aerobic mineralization of cis-1,2-dichloroethylene. | metabolically engineered escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-dce). the strains express the six genes of an evolved toluene ortho-monooxygenase from burkholderia cepacia g4 (tom-green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (gshi, which forms glutathione) and the glutathione s-transferase isoilr1 from rhodococcus ad45 (which adds glutathione to the reactive cis-dce epoxide) or (2) with an evolved epoxide hy ... | 2006 | 16739990 |
| internally controlled real-time pcr method for quantitative species-specific detection and vapa genotyping of rhodococcus equi. | we developed a novel quantitative real-time pcr (q-pcr) method for the soil actinomycete rhodococcus equi, an important horse pathogen and emerging human pathogen. species-specific quantification was achieved by targeting the chromosomal monocopy gene choe, universally conserved in r. equi. the choe q-pcr included an internal amplification control (iac) for identification of false negatives. a second q-pcr targeted the virulence plasmid gene vapa, carried by most horse isolates but infrequently ... | 2006 | 16751540 |
| lariatins, antimycobacterial peptides produced by rhodococcus sp. k01-b0171, have a lasso structure. | two antimycobacterial agents, lariatins a and b, were isolated from the culture broth of rhodococcus sp. k01-b0171. their structures were elucidated by spectral analysis and advanced protein chemical methods to be unique cyclic peptides, which consist of 18 and 20 l-amino acid residues with an internal linkage between the gamma-carboxyl group of glu8 and the alpha-amino group of gly1. the three-dimensional structure of lariatin a deduced from nmr data by dynamical simulated annealing method indi ... | 2006 | 16756302 |
| [the prospects of using bacteria of the genus rhodococcus and microbial surfactants for the degradation of oil pollutants]. | the possibility of accelerating oil degradation by an enrichment culture of oil-oxidizing microorganisms in the presence of bacteria of the genus rhodococcus and microbial surfactants was studied. it was shown that the degree of consumption of crude oil (2% v/v) after 192 h of enrichment culture growth reached 84%. inoculation of the active hydrocarbon-oxidizing strain rhodococcus erythropolis ek-1 and exogenous surfactants produced by pseudomonas sp. ps-27 increased this degree to 90% and 93-94 ... | 2006 | 16761570 |
| bioremediation of environmental endocrine disruptor di-n-butyl phthalate ester by rhodococcus ruber. | in this study dbp-degradation strain cq0301 was isolated from rubbish landfill soil. according to the biophysical, biochemical characteristics and analysis of 16s rrna, the strain was identified as rhodococcus ruber. three new protein bands could be fractioned after dbp-inducing, which were suspected to participate the process of dbp-degrading. catechol was suspected to be an intermediate product of dbp and cleaving the benzene ring was catalyzed by catechol 1,2-dioxygenase, because a highly act ... | 2006 | 16762396 |
| rhodococcus equi--an emerging human pathogen in immunocompromized hosts: a report of four cases from malaysia. | rhodococcus equi, a recognized pathogen in horses, is emerging as a human opportunistic pathogen, especially in immunocompromized hosts. we describe four immunocompromized patients who had serious r. equi infections with an overall mortality of 75%. the natural habitat of r. equi is soil, particularly soil contaminated with animal manure. necrotizing pneumonia is the commonest form of infection but extrapulmonary infections, such as wound infections and subcutaneous abscess, have also been descr ... | 2006 | 16771229 |
| isolation and characterization of a carbendazim-degrading rhodococcus sp. djl-6. | bacterium djl-6, capable of degrading carbendazim, was isolated by continuous enrichment culture originating from carbendazim-treated soil. the isolate was identified as rhodococcus sp. according to its phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. the strain could use carbendazim as sole carbon or nitrogen source. it showed a high average degradation rate of 55.56 mg . l(-1) . d(-1) in m9 medium amended with carbendazim. high-pressure liquid chroma ... | 2006 | 16775791 |
| interaction of metabolites with r. rhodochrous during the biodegradation of di-ester plasticizers. | the commonly used plasticizers di-ethylhexyl phthalate (dehp) and di-ethylhexyl adipate (deha) are known to partially degrade in the presence of soil microorganisms, such as rhodococcus rhodochrous, releasing persistent and toxic metabolites. the metabolites adipic acid and 2-ethylhexanol were both shown to inhibit growth of the degrading microbe. 2-ethylhexanol enhanced the activity of ethanol dehydrogenase - an enzyme involved in its metabolism - but the activity of this enzyme was inhibited b ... | 2006 | 16777176 |
| resuscitation-promoting factors: distribution among actinobacteria, synthesis during life-cycle and biological activity. | we have identified new members of the resuscitation-promoting factor (rpf) protein family in numerous as yet unsequenced actinobacteria suggesting that rpfs are common in this bacterial group. formation and final delivery of the rpf proteins varied from strain to strain and were dependent on growth conditions. rpfs were found to act complementary to resuscitate other actinobacteria and might therefore be useful tools for the isolation of new actinobacterial species, especially from soil samples ... | 2006 | 16779632 |
| immunogenicity of synthetic rhodococcus equi virulence-associated protein peptides in neonate foals. | rhodococcus equi infection is considered the most common cause of pneumonia in foals less than 6 months of age. immunization of foals and/or mares may become a procedure of choice for prevention. the present work documents the antibody response of neonate foals to r. equi virulence-associated protein (vap) vaccine candidate peptides. a mixture of 4 r. equi (atcc 33701) vap peptides was selected based on their hydrophilicity and recognition by naturally acquired igg antibodies from 13 adult horse ... | 2006 | 16782401 |
| a regulatory effect of the balance between tnf-alpha and il-6 in the granulomatous and inflammatory response to rhodococcus aurantiacus infection in mice. | after i.v. inoculation with rhodococcus aurantiacus, wild-type (wt) mice develop nonnecrotic, epithelioid granulomas. because a high level of tnf-alpha is observed during the initial phase postinfection, we examined the extent to which tnf-alpha contributes to granulomatous inflammation using tnf-alpha gene-deficient (tnf-alpha(-/-)) mice. despite a lack of r. aurantiacus proliferation, tnf-alpha(-/-) mice displayed high mortality rates within 5 days postinfection, as well as a high level of il- ... | 2006 | 16785562 |
| caver: a new tool to explore routes from protein clefts, pockets and cavities. | the main aim of this study was to develop and implement an algorithm for the rapid, accurate and automated identification of paths leading from buried protein clefts, pockets and cavities in dynamic and static protein structures to the outside solvent. | 2006 | 16792811 |
| crystal structure of 3-chlorocatechol 1,2-dioxygenase key enzyme of a new modified ortho-pathway from the gram-positive rhodococcus opacus 1cp grown on 2-chlorophenol. | the crystal structure of the 3-chlorocatechol 1,2-dioxygenase from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, a fe(iii) ion-containing enzyme specialized in the aerobic biodegradation of 3-chloro- and methyl-substituted catechols, has been solved by molecular replacement techniques using the coordinates of 4-chlorocatechol 1,2-dioxygenase from the same organism (pdb code 1s9a) as a starting model and refined at 1.9 a resolution (r(free) 21.9%; r-factor 17.4%). the analysi ... | 2006 | 16793061 |
| overcoming the thermodynamic limitation in asymmetric hydrogen transfer reactions catalyzed by whole cells. | whole lyophilized cells of an escherichia coli overexpressing the alcohol dehydrogenase (adh-'a') from rhodococcus ruber dsm 44541 were used for the asymmetric reduction of ketones to secondary alcohols. the recycling of the required nicotinamide cofactor (nadh) was achieved in a coupled-substrate process. in the course of the reaction the ketone is reduced to the alcohol and the hydrogen donor 2-propanol is oxidized to acetone by one enzyme. this leads to a thermodynamic equilibrium between all ... | 2006 | 16804944 |
| desulfurization of dibenzothiophene by bacillus subtilis recombinants carrying dszabc and dszd genes. | the desulfurization (dsz) genes from rhodococcus erythropolis ds-3 were successfully integrated into the chromosomes of bacillus subtilis atcc 21332 and uv1 using an integration vector pdgsdn, yielding two recombinant strains, b. subtilis m29 and m28 in which the integrated dsz genes were expressed efficiently under the promoter, pspac. the dibenzothiophene (dbt) desulfurization efficiency of m29 was 16.2 mg dbt l(-1) h(-1) at 36 h, significantly higher than that of r. erythropolis ds-3 and b. s ... | 2006 | 16810451 |
| biodesulfurization in biphasic systems containing organic solvents. | biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. a solvent-tolerant desulfurizing bacterium, pseudomonas putida a4, was constructed by introducing the biodesulfurizing gene cluster dszabcd, which was from rhodococcus erythropolis xp, into the solvent-tolerant strain p. putida idaho. biphasic reactions were performed t ... | 2006 | 16820450 |
| development of a microtiter plate-based method for determination of degradation profile of nitrophenolic compounds. | a microtiter plate-based assay was developed for the automatic monitoring of degradation profile of the yellow-coloured nitrophenolic compounds. the method enables to reduce the intervals between measurements of substrate concentration to minutes and to overcome the problem of discontinuity of sampling typical for conventional methods. the concentrations of nitrophenolic compounds were calculated from the absorbance values determined automatically by bioscreen c. verification of the method was b ... | 2006 | 16289394 |
| characterization of four rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols. | four genes were isolated and characterized for alcohol dehydrogenases (adhs) catalyzing the oxidation of aromatic alcohols such as benzyl alcohol to their corresponding aldehydes, one from o-xylene-degrading rhodococcus opacus tkn14 and the other three from n-alkane-degrading rhodococcus erythropolis pr4. various aromatic alcohols were bioconverted to their corresponding carboxylic acids using escherichia coli cells expressing each of the four adh genes together with an aromatic aldehyde dehydro ... | 2006 | 16292529 |
| metabolites from the biodegradation of di-ester plasticizers by rhodococcus rhodochrous. | the plasticizers di-2-ethylhexyl phthalate (dehp) and di-2-ethylhexyl adipate (deha) are ubiquitous in the environment and undergo partial biodegradation in the presence of soil micro-organisms. the validity of a proposed pathway for the degradation of these plasticizers by rhodococcus rhodochrous has been confirmed by the identification of 2-ethylhexanal in gas phase emissions. complete analyses of the aqueous and gas phases were able to account for more than 98% of the 2-ethylhexanol component ... | 2006 | 16316677 |
| immobilization of hydrocarbon-oxidizing bacteria in poly(vinyl alcohol) cryogels hydrophobized using a biosurfactant. | a simple biosurfactant-based hydrophobization procedure for poly(vinyl alcohol) (pva) cryogels was developed allowing effective immobilization of hydrocarbon-oxidizing bacteria. the resulting partially hydrophobized pva cryogel granules (granule volume 5 microl) contained sufficient number (6.5 x 10(3)) of viable bacterial cells per granule, possessed high mechanical strength and spontaneously located at the interface in water-hydrocarbon system. such interfacial location of pva granules allowed ... | 2006 | 16316701 |
| characterization of the putative operon containing arylamine n-acetyltransferase (nat) in mycobacterium bovis bcg. | mycobacterium bovis bcg and mycobacterium tuberculosis possess a single arylamine n-acetyltransferase whose gene is predicted to occur within a six-gene operon. deletion of the nat gene caused an extended lag phase in m. bovis bcg and a cell morphology associated with an altered pattern of cell wall mycolates. analysis of cdna from m. bovis bcg shows that during in vitro growth all the genes in the putative nat operon are expressed and the open reading frames are contiguous, supporting the exist ... | 2006 | 16359327 |
| gene expression analysis using a modified hicep method applicable to prokaryotes: a study of the response of rhodococcus to isoniazid and ethambutol. | we established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the hicep (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. following the removal of abundant rrna, the mrna of prokaryotic cells was enzymatically polyadenylated and subjected to hicep analysis. this method was highly reproducible due to selective pcr that was performed by using adaptor specific primers. we confirmed indu ... | 2006 | 16360900 |
| rhodococcus ruber as a cause of keratitis. | to report a case of suppurative keratitis caused by rhodococcus ruber in a healthy 31-year-old male patient. | 2006 | 16371792 |
| bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae). | wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ... | 2006 | 16382282 |
| deep desulfurization of diesel oil and crude oils by a newly isolated rhodococcus erythropolis strain. | the soil-isolated strain xp was identified as rhodococcus erythropolis. r. erythropolis xp could efficiently desulfurize benzonaphthothiophene, a complicated model sulfur compound that exists in crude oil. the desulfurization product of benzonaphthothiophene was identified as alpha-hydroxy-beta-phenyl-naphthalene. resting cells could desulfurize diesel oil (total organic sulfur, 259 ppm) after hydrodesulfurization. the sulfur content of diesel oil was reduced by 94.5% by using the resting cell b ... | 2006 | 16391024 |
| aerobic biodegradation of 2,2'-dithiodibenzoic acid produced from dibenzothiophene metabolites. | dibenzothiophene is a sulfur heterocycle found in crude oils and coal. the biodegradation of dibenzothiophene through the kodama pathway by pseudomonas sp. strain bt1d leads to the formation of three disulfides: 2-oxo-2-(2-thiophenyl)ethanoic acid disulfide, 2-oxo-2-(2-thiophenyl)ethanoic acid-2-benzoic acid disulfide, and 2,2'-dithiodibenzoic acid. when provided as the carbon and sulfur source in liquid medium, 2,2'-dithiodibenzoic acid was degraded by soil enrichment cultures. two bacterial is ... | 2006 | 16391083 |