Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [diphtheria--isolation of an endemic corynebacterium diphtheriae strain in southern lower saxony]. | after decades of only limited epidemiological and clinical importance diphtheria (especially of the upper respiratory tract) is regaining increasing interest of public health officials, as recent case reports in national and international publications clearly demonstrate. in march 1993 we observed a case of tracheobronchial corynebacterium diphtheriae mitis infection in a 66-year old man from the south of lower saxony with clinical presentation of mucopurolent exacerbation of chronic bronchitis. ... | 1993 | 8111165 |
| [the vaccinal prevention of diphtheria and tetanus in children suffering from bronchial asthma and asthmatic bronchitis]. | the course of the vaccinal process was studied in 69 children with bronchial asthma and asthmatic bronchitis after the injection of adsorbed diphtheria-tetanus (dt) toxoid with reduced antigen content. after immunization all these children were found to form protective levels of anti-diphtheritic and antitetanus antibodies irrespective of the severity and duration of the remission of the main disease prior to vaccination; at the same time no obstructive changes in bronchial ventilation were obse ... | 1993 | 8067097 |
| non-toxigenic corynebacterium diphtheriae in military personnel. | 1993 | 8096897 | |
| non-toxigenic corynebacterium diphtheriae var gravis in england. | 1993 | 8099660 | |
| diphtheria and travel. | 1993 | 8101955 | |
| prevalence of diphtheria and tetanus antibodies in young adults in delhi. | a total of 574 blood samples collected mainly from adult males, on a random basis, were tested for diphtheria and tetanus antibodies by indirect haemagglutination (iha) test to find out the percentage of individuals with protective titres (> or = 0.015 iu/ml). a total of 502 (87.5 per cent) and 437 (76.2 per cent) of these had protective titres against diphtheria and tetanus respectively. the vaccination status of these subjects against diphtheria and tetanus was not ascertainable. the relevance ... | 1993 | 8014436 |
| a diphtheria epidemic in lesotho, 1989. did vaccination increase the population's susceptibility? | we report a diphtheria outbreak which occurred in the 1st 6 months of 1989 in the quthing district of lesotho in southern africa. sixty-eight clinical cases were identified in patients with ages ranging from 14 months to 51 years. the 10-15-year age group represented 38% of the cases and the 15-20-year age group another 26%. age-adjusted incidence rates were higher in patients aged between 15 and 35 than in those aged 0-15 years (p < 0.001). the overall case fatality rate was 23%. most deaths oc ... | 1993 | 7681640 |
| diphtheria in the united kingdom--two recent incidents. | 1993 | 7691357 | |
| [the circulation of corynebacterium diphtheriae within the current epidemiological context]. | 1993 | 9132114 | |
| corynebacterium diphtheriae, skin ulcers and travel abroad. | 1992 | 1285147 | |
| non-toxigenic corynebacterium diphtheriae var gravis. | 1992 | 1285166 | |
| a case of cutaneous diphtheria. | 1992 | 1285171 | |
| [the efficacy of the adtp-vaccine immunization of children with different courses of the vaccinal period]. | 1992 | 1291954 | |
| [cutaneous diphtheria in a patient with hiv infection]. | 1992 | 1301708 | |
| [surveillance of adverse effects following immunization (aefi)]. | the meeting of working group on adverse events following immunization (aefi) was organized in paris on february 5-6-7 1992. reporting and surveillance of aefi in the european countries was advised to be introduced in each country as the part of monitoring system in this matter. channels for reporting system of aefi should be similar to channels for reporting system of communicable diseases or to system of events following drugs. reporting and surveillance system of aefi in poland as well as prop ... | 1992 | 1335588 |
| immunogenicity of combined diphtheria, tetanus, and pertussis vaccine given at 2, 3, and 4 months versus 3, 5, and 9 months of age. | in the uk an accelerated schedule for immunisation against diphtheria, tetanus, and pertussis (injections at 2, 3, and 4 months of age) was introduced in 1990 to replace the more widely spaced schedule of 3, 5, and 9 months. there is concern, however, that the new schedule may be less immunogenic and therefore less protective than the old schedule. we have measured serum concentrations of antibodies against diphtheria, pertussis, and tetanus in infants immunised according to the two regimens. bo ... | 1992 | 1346876 |
| unusual non-toxigenic corynebacterium diphtheriae in homosexual men. | 1992 | 1348837 | |
| invasive infection with non-toxigenic corynebacterium diphtheriae among drug users. | 1992 | 1350021 | |
| [cytopathogenic effect of diphtheria exotoxin in cultured chick embryo fibroblasts]. | one hundred fifty strains of corynebacterium diphtheriae were studied from patients with symptoms of diphtheria and from healthy persons. 136 strains (90.67%) belonged to the mitis biotype, 12 (8.0%) and 2 (1.33%) to gravis and intermedius biotypes respectively. toxigenicity was determined by traditional in vivo methods with rabbits and plaque immunodiffusion in kl-virulence medium. from the 136 mitis biotype strains studied, 130 (95.58%) showed positive toxigenicity by both methods and 11 strai ... | 1992 | 1391077 |
| molecular epidemiology of nasopharyngeal corynebacteria in healthy adults from an area where diphtheria vaccination has been extensively practiced. | in addition to conventional biochemical tests, a dna probe specific for corynebacterium diphtheriae was used to characterize 53 cystinase-positive and urease-negative corynebacteria strains isolated from pharyngeal and nasal swabs obtained from 515 healthy adults living in an urban area of central italy. no corynebacterium diphtheriae strain was found. six "atypical" strains were isolated, which could not be classified in any of the species so far defined in the corynebacterium genus. these stra ... | 1992 | 1397226 |
| binding of the metalloregulatory protein dtxr to the diphtheria tox operator requires a divalent heavy metal ion and protects the palindromic sequence from dnase i digestion. | transcription of the corynebacteriophage diphtheria tox operon has been shown to be regulated through a corynebacterial determined factor dtxr. the dtxr gene has been recently cloned and expressed in escherichia coli, and shown to regulate the expression of beta-galactosidase expression from a diphtheria tox promoter/operator-lacz transcriptional fusion. tao et al. (tao, x., boyd, j., and murphy, j. r. (1992) proc. natl. acad. sci. u.s.a. 89, 5897-5901) have recently shown by gel mobility shift ... | 1992 | 1400485 |
| fatal septicemia due to a toxigenic strain of corynebacterium diphtheriae subspecies mitis. | 1992 | 1425741 | |
| controlled study of a new five-component acellular pertussis vaccine in adults and young children. | a new five-component acellular pertussis (ap) vaccine containing 10 micrograms of pertussis toxoid, 5 micrograms of filamentous hemagglutinin, 5 micrograms of combined agglutinogens 2 and 3, and 3 micrograms of pertactin was evaluated in adults and young children. ap vaccine was compared with saline placebo in 31 adults, and ap vaccine combined with diphtheria and tetanus toxoids (adtp) was compared with whole cell dtp in 41 children, ages 16-20 months, who had received whole cell dtp during inf ... | 1992 | 1431261 |
| immunological response to diphtheria/tetanus vaccine in schistosomiasis mansoni patients. | the cellular and humoral immune responses of patients with s. mansoni infection were evaluated before and one month after each of two intramuscular doses of diphtheria/tetanus toxoid vaccine. patients were divided into "responder" and "non-responder" groups based on anti-tetanus toxoid (anti-tt) igg levels after vaccination. the specific anti-tt igg1 response of the responder group was predominantly in the igg, subclass. the lymphoproliferative response to pha was also significantly higher in th ... | 1992 | 1431294 |
| screening for corynebacterium diphtheriae. | a throat swab from a 9 year old girl with pharyngitis yielded a non-toxigenic strain of corynebacterium diphtheriae var mitis and streptococcus group g. c pseudodiphtheriticum was isolated from the throats of two of her four brothers. in each case the isolate was sent to the reference laboratory before full identification. the growth was found to be mixed for one brother; the other isolate being a toxin producing c diphtheriae var gravis. the child was asymptomatic and the case proves that all c ... | 1992 | 1452782 |
| transcription analysis and nucleotide sequence of tox promoter/operator mutants of corynebacteriophage beta. | the production of diphtheria toxin (dt) by corynebacterium diphtheriae c7 (beta) is transcriptionally regulated by the iron-dependent diphtheria toxin repressor, dtxr. transcription of the tox gene was studied in wild-type c. diphtheriae c7 (beta) and in lysogens carrying mutants of beta that determine insensitivity to inhibition of dt production by iron. under low iron conditions in all strains, tox-specific mrna appeared and dt production began during late-log phase, and they increased to maxi ... | 1992 | 1453929 |
| [the use of samples of capillary blood absorbed on paper filters for evaluating the levels of antidiphtheria and antitetanus immunity in seroepidemiological surveillance]. | 1992 | 1472782 | |
| diphtheria: another risk of travel. | a 19-year-old woman presented with cellulitis of her foot 10 days after returning from bali. swabs of a central necrotic area grew toxigenic corynebacterium diphtheriae biotype gravis. the patient was treated with parenteral penicillin and made a complete recovery. diphtheria immunisation should be regularly updated for travellers to the tropics. clinical and laboratory recognition of this infection is essential for appropriate public health measures to be undertaken. | 1992 | 1474268 |
| [the characteristics of population immunity to measles and diphtheria in adolescent collectives]. | population immunity to measles and diphtheria was evaluated among adolescents of a professional-technical school. low indices of immunity were found. there was a clear difference in the number of girls and boys with antibody titers to measles virus and diphtheria anatoxin (dilution 1:40) as well as in the number of non-immune to diphtheria. a clear increase of the number of students with protective titers by the end of the school year evidences presence of latent epidemic process of measles and ... | 1992 | 1475928 |
| molecular weight determination of methyl esters of mycolic acids using thermospray mass spectrometry. | methyl esters of normal fatty acids, corynomycolate and corynomycolenate were used as model compounds for thermospray mass spectrometric procedures for molecular weight determination of the related nocardial mycolic acids. by using ammonium acetate at the positive ion generator, in both cases, a family of ions was produced. the following members were found and corresponded to the adducts: (1) m + h; m + nh4 and m + h + nh4 for methyl esters of normal fatty acids, whereas m + h, m + 2h and m + h ... | 1992 | 1486659 |
| a rapid, simple method for detecting pcr failure. | 1992 | 1490181 | |
| antibacterial spectrum of lactoferricin b, a potent bactericidal peptide derived from the n-terminal region of bovine lactoferrin. | a physiologically diverse range of gram-positive and gram-negative bacteria was found to be susceptible to inhibition and inactivation by lactoferricin b, a peptide produced by gastric pepsin digestion of bovine lactoferrin. the list of susceptible organisms includes escherichia coli, salmonella enteritidis, klebsiella pneumoniae, proteus vulgaris, yersinia enterocolitica, pseudomonas aeruginosa, campylobacter jejuni, staphylococcus aureus, streptococcus mutans, corynebacterium diphtheriae, list ... | 1992 | 1490908 |
| characterization of antibiotic-resistant corynebacterium striatum strains. | antibiotic-resistant corynebacterium strains were isolated from 14 harborview and one veterans administration hospital patients in seattle during the period 1987-90. these clindamycin-erythromycin resistant strains were shown to hybridize with the ermcd gene, which was cloned from a corynebacterium diphtheriae plasmid and encodes for a rrna methylase. thirteen of these strains also hybridized with the tetm gene probes, and were tetracycline resistant. the ermcd gene could be transferred, by conj ... | 1992 | 1490919 |
| [corynebacterium diphtheriae septicemia in a child with congenital cardiopathy]. | 1992 | 1492087 | |
| purification and characterization of the diphtheria toxin repressor. | the diphtheria toxin repressor gene (dtxr) encodes a protein (dtxr) that regulates transcription of the diphtheria toxin gene (tox) by an iron-dependent mechanism. cloned dtxr was expressed in escherichia coli from the phage t7 gene 10 promoter, and dtxr was purified. specific binding of dtxr to the tox+ operator was dependent on reduction of dtxr and the presence of ferrous ions. dtxr protected a sequence of approximately 30 nucleotide pairs, partially overlapping the tox promoter and containin ... | 1992 | 1502169 |
| [diphtheria immunity in school beginners and after reimmunization with d-vaccine]. | the antitoxin immune status against diphtheria was determined in 814 children, aged 6-7 years, 5-6 years after first immunization. all children were then re-immunized with d-vaccine. 4-8 weeks later antitoxin was again measured to assess the immunogenic effect of the booster injection (reduced toxoid dosage of 5 iu/0.5 ml). although all children had been completely immunized as infants, 149 of them (18.3%) presented inadequate or no immunity to diphtheria. the booster injection resulted in a rel ... | 1992 | 1505351 |
| selection for immune response in goats: the effect of immunization procedure on antibody response to diphtheria toxoid and human serum albumin. | the serum antibody titers to diphtheria toxoid and human serum albumin were determined in 103 goat kids from lines selected for 12 yr for high or low antibody response to diphtheria toxoid. in the 12th yr, six groups of kids were immunized with different preparations of the antigens. in all groups but one, the antigens were emulsified in freund's incomplete adjuvant with added sonicated mycobacterium paratuberculosis. the groups received the following treatments: group 1 was immunized with both ... | 1992 | 1526911 |
| [study on immune persistency of combined diphtheria-pertussis-tetanus (dpt) triple vaccine]. | immune efficacy and persistency were studied by comparison of two-dose immunization of adsorbed dpt at one and two month interval and two & three dose immunization of unadsorbed dpt during 1970s and 1980s. serological investigation showed that antibody response to diphtheria, tetanus were elicited in four groups of children after primary immunization, but, the antibody response to pertussis was poor in children immunized with unadsorbed dpt. three kinds of antibody titres were increased signific ... | 1992 | 1581951 |
| the study of agglutination of trypsin-treated sheep red cells by corynebacterium diphtheriae strains. | 620 corynebacterium diphtheriae strains from 472 sick and healthy persons were studied for their adhesive activity (aa) in direct agglutination of trypsin-treated sheep erythrocytes. toxigenic strains had more active aa than non-toxigenic ones which was not dependent on the presence of toxin in the culture. neither biotype nor serotype of the strains correlated with their aa. several lysotypes among toxigenic and non-toxigenic strains were more active than others. toxigenic strains from patients ... | 1992 | 1599709 |
| clinical reactions and immunogenicity of the biken acellular diphtheria and tetanus toxoids and pertussis vaccine in 4- through 6-year-old us children. | objective--to compare the immunogenicity and reactogenicity of a two-component acellular pertussis vaccine with a whole-cell diphtheria and tetanus toxoids and pertussis vaccine (w-dtp) when administered as a booster to children 4 through 6 years of age. design--this was a randomized, double-blind study. setting--children in this study were from three general pediatric practices (two were private, one was university-affiliated). participants--three hundred and sixteen 4- through 6-year-old child ... | 1992 | 1621656 |
| diphtheria toxin and pseudomonas aeruginosa exotoxin a: active-site structure and enzymic mechanism. | 1992 | 1628498 | |
| specific binding of the diphtheria tox regulatory element dtxr to the tox operator requires divalent heavy metal ions and a 9-base-pair interrupted palindromic sequence. | the structural gene for diphtheria toxin, tox, is carried by a family of closely related corynebacteriophages; however, the regulation of tox expression is controlled by a corynebacterium diphtheriae-encoded regulatory element, dtxr. the molecular cloning and sequence analysis of dtxr was recently described. previous studies have suggested that dtxr-mediated regulation of the diphtheria tox operator involves the formation of an iron-repressor complex, which specifically binds to the tox operator ... | 1992 | 1631071 |
| diphtheria toxin cloning and expression in corynebacterium diphtheriae. | 1992 | 1633295 | |
| the structure of diphtheria toxin as a guide to rational design. | 1992 | 1633298 | |
| cytotoxic activity of a recombinant chimaeric protein between pseudomonas aeruginosa exotoxin a and corynebacterium diphtheriae diphtheria toxin. | a segment of the exotoxin a gene of pseudomonas aeruginosa, coding for the n-terminal end of domain i and domain ii of the toxin (eta), was genetically fused to the diphtheria toxin gene of corynebacterium diphtheriae, coding for the n-terminal end of a fragment of diphtheria toxin (dt). the resulting hybrid protein (termed ced1) was produced in large amounts and exported to the periplasm in escherichia coli. this chimaeric protein reacted with both anti-eta and anti-dt antisera. furthermore, th ... | 1992 | 1640830 |
| impact of haemophilus influenzae type b polysaccharide-tetanus protein conjugate vaccine on responses to concurrently administered diphtheria-tetanus-pertussis vaccine. | to assess whether serum antibody responses to diphtheria-tetanus-pertussis (dtp) vaccine were affected by coadministration of haemophilus influenzae type b capsular polyribosylribitol phosphate polysaccharide-tetanus protein (prp-t) conjugate vaccine when given to patients at 2, 4, and 6 months of age. | 1992 | 1731134 |
| dna sequences and characterization of dtxr alleles from corynebacterium diphtheriae pw8(-), 1030(-), and c7hm723(-). | the structural gene encoding dtxr, an iron-dependent diphtheria tox regulatory element, has recently been cloned and sequenced from the c7(-) strain of corynebacterium diphtheriae (j. m. boyd, m. oza, and j. r. murphy, proc. natl. acad. sci. usa 87:5972, 1990). we report here the molecular cloning, dna sequence analysis, and characterization of dtxr from the pw8(-), 1030(-), and c7hm723 strains of c. diphtheriae. while the sequence of dtxr from pw8(-) is identical to that of the c7(-) allele, th ... | 1992 | 1735717 |
| screening tests for pathogenic corynebacteria. | to provide simple tests that would help in the identification of corynebacteria that produce diphtheria toxin. | 1992 | 1740514 |
| immunity to diphtheria in the 3-19 year age group in italy. | in italy, immunization with diphtheria toxoid has been compulsory for all newborns since 1939. the last two clinical cases of diphtheria were reported in 1987. during the period 1987-1989, immunity against diphtheria was assessed by neutralization test in a random sample of 1740 healthy subjects 3-19 years old, from five geographical areas of italy. of the total population, 76.5% showed antibody levels considered to be protective (greater than or equal to 0.1 iu ml-1), 17.2% had a relative degre ... | 1991 | 1759506 |
| [the use of the cluster selection method for assessing the status of immunity to controlled infections (measles, diphtheria and tetanus)]. | the use of the method of cluster selection in mass serological examination makes it possible to obtain statistically significant results with very low expenditures. the coincidence of the results on the number of seronegative children, obtained by the method of cluster selection, with the results of mass serological examination of all children aged 6-7 years in the district (children found to be seronegative to measles constituted 5.5% and 5.0% respectively) is indicative of the statistical sign ... | 1991 | 1759518 |
| [the vaccination of children with allergic diseases with reduced doses of adsorbed dt-m anatoxin]. | during a remission and drug therapy, 71 children suffering from allergic diseases were vaccinated with dt-m toxoid containing different dose of diphtheria (1, 2.5, 5 lf) and tetanus (1, 2.5, 5 ec) antigens. it has been shown that all the children responded to tetanus antigen by adequate serological tests, whereas as regards diphtheria toxoid, the patients immunized with the least dose of dt-m preparation (1 lf + + 1 ec antigens) produced specific antibodies later and at a lower level. during the ... | 1991 | 1762853 |
| rapid screening for toxigenic corynebacterium diphtheriae by the polymerase chain reaction. | the polymerase chain reaction (pcr) was used to discriminate between toxigenic and non-toxigenic isolates of corynebacterium diphtheriae. primers specific to the diphtheria toxin gene were used to amplify a toxin gene fragment from simple boiled-cell preparations. eight recent clinical isolates and four reference strains were tested. the result of the pcr agreed with the traditional toxigenicity assays (the elek test and guinea pig inoculation) in all cases. pcr has several advantages over the e ... | 1991 | 1791203 |
| vaccine-preventable respiratory infections in childhood. | in the past decade, immunization rates among preschool-age children in the united states have decreased to levels lower than those in many developing countries. as a result, epidemics of vaccine-preventable diseases have occurred, especially in urban areas. six of the infections prevented by immunization--those caused by bordetella pertussis, streptococcus pneumoniae, haemophilus influenzae type b, corynebacterium diphtheriae, measles virus, and influenza virus--frequently cause respiratory trac ... | 1991 | 1809999 |
| [the adhesive activity of diphtheritic strains in relation to the characteristics of the infectious process they induce]. | the degree of adhesiveness of 602 c. diphtheriae strains isolated from patients with different forms of diphtheria was studied on trypsinized sheep red blood cells (srbc) used as an experimental model. the titer of bacterial suspension, i.e. its highest dilution ensuring the agglutination of 50% of srbc, was assumed to be the index of adhesive activity. the toxigenic strains were more homogeneous with respect to the degree of their activity and proved to be moderately and highly adhesive, while ... | 1991 | 1811384 |
| cutaneous diphtheria. | 1991 | 1816125 | |
| relationship of biotype and source to the hemagglutination and adhesive properties of c. diphtheriae. | 1. a study was conducted on the hemagglutinating and adhesive properties of sucrose-fermenting and non-sucrose-fermenting corynebacterium diphtheriae strains. sheep erythrocytes were agglutinated by 72% of toxigen c. diphtheriae strains and by 87% of nontoxigenic strains, by 67% of the strains isolated from throats and 94% of skin isolates, and by 65% of sucrose-fermenting organisms and 93% of non-sucrose-fermenting strains. 2. neither presence nor absence of toxin production was correlated with ... | 1991 | 1823253 |
| iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned corynebacterium diphtheriae repressor gene dtxr in c. diphtheriae c7 strains. | a regulatory gene (dtxr) responsible for iron-dependent repression of the toxin (tox) and siderophore genes in corynebacterium diphtheriae was cloned and characterized. a dna fragment carrying dtxr repressed expression of a tox-lacz gene fusion in escherichia coli dh5 alpha in a high-iron environment but not under low-iron conditions. a protein with mobility corresponding to approximately 28 to 29 kda was identified as the product of the dtxr gene by sodium dodecyl sulfate-polyacrylamide gel ele ... | 1991 | 1828057 |
| [the effect of nematode invasiveness in children on postvaccinal immunity]. | 1991 | 1836830 | |
| periodic acid-schiff-positive organisms in primary cutaneous bacillus cereus infection. case report and an investigation of the periodic acid-schiff staining properties of bacteria. | primary cutaneous bacillus cereus infection frequently presents as a single necrotic bulla on the extremity of an immunocompromised patient. in lesional biopsy specimens and smears, the large gram-positive rods of b cereus may be mistaken for clostridium species. this is a potentially serious error, as bacillus species are resistant to penicillin and other beta-lactam antibiotics. we studied a case in which large periodic acid-schiff-staining organisms were seen in the biopsy specimen from a nec ... | 1991 | 1900984 |
| [diphtheria in denmark 1956-1989. occurrence of corynebacterium diphtheriae and other diphtheria toxigenic bacteria]. | the public immunization program against diphtheria, established in 1941, has almost eradicated the disease in denmark, and 1956 became the first year without any notified cases. since then, toxigenic strains have only been isolated five times--three cases of clinical diphtheria due to corynebacterium diphtheriae biovar. mitis and two cases of tonsillitis/pharyngitis due to corynebacterium ulcerans. the source of the infection was not identified in any of the cases. the first case of diphtheria i ... | 1991 | 1901182 |
| comparison of acellular and whole-cell pertussis-component diphtheria-tetanus-pertussis vaccines in infants. the apdt vaccine study group. | in a multicenter, double-blind, randomized, longitudinal study, 252 children received licensed lederle diphtheria-tetanus toxoids and pertussis vaccine adsorbed (dtp) at 2, 4, and 6 months of age, and 245 children received a dtp vaccine with the lederle/takeda acellular pertussis component (apdt) at the same ages. both groups of children received apdt vaccine at 18 months of age. after each of the first three immunizations, apdt vaccine recipients had fewer local and systemic reactions than did ... | 1991 | 1907317 |
| microbial lipids and stable foam formation in the activated sludge process. | the presence of fats and oils in sewage has been related to the formation of stable foams in activated sludge treatment systems. foam forming microbes can utilise and, in some cases, store lipid substrates. since surface lipids would confer the hydrophobicity necessary for flotation on the sludge biomass, the extractable lipids in foaming and non-foaming biomass samples were examined. both pure mono-cultures and sludge samples were used. the results showed that, whilst there were some difference ... | 1991 | 1907713 |
| [the characteristics of corynebacterium diphtheriae adhesins]. | the study of the properties of c. diphtheriae adhesins revealed the absence of their thermostability, which suggested the protein nature of these adhesins. c. diphtheriae also showed pronounced mannose-resistant hemagglutinating activity, as this activity remained unchanged even in the presence of d-mannose. the comparison of the adhesive activity of c. diphtheriae with their phage and corycine sensitivity revealed essential differences between these macroorganisms in the degree of their adhesiv ... | 1991 | 1909075 |
| [the clinico-epidemiological characteristics of diphtheria in adults]. | of 262 patients referred with suspicion of diphtheria the diagnosis was confirmed in 145 (55.3%). clinico-epidemiological data indicate that the severity of diphtheria course, the tendency of increase of its incidence at the ukrssr territory and in the ussr emphasize that early diagnosis and adequate treatment are of prime importance. to achieve this not only epidemiologists and infectionists but also cardiologists, otolaryngologists and general practitioners should actively participate in manag ... | 1991 | 1949721 |
| [a comparative evaluation of the immunological efficacy of adpt vaccine and adt-m anatoxin in the immunization of children against diphtheria and tetanus]. | comparative study of the immunological effectiveness of adsorbed diphtheria-pertussis-tetanus (dpt) vaccine and adsorbed diphtheria-tetanus toxoid with reduced antigen content (adsorbed dt toxoid r) in the immunization of children, carried out in accordance with the vaccination schedule, was made. immune response to the injection of adsorbed dpt vaccine was higher than after immunization with adsorbed dt toxoid r, as evidenced by antibody titers. it was probably due to differences in the number ... | 1991 | 1950266 |
| immunogenicity of haemophilus influenzae type b conjugate vaccines in infant rhesus monkeys. | two haemophilus influenzae type b (hib) polysaccharide-protein conjugate vaccines were evaluated for immunogenicity in eliciting anti-polyribosyl ribitol phosphate (prp) antibodies in infant rhesus monkeys. animals received intramuscular injections of either hib polysaccharide (prp)-meningococcal outer membrane protein complex or hib oligosaccharide-crm197 (hboc) conjugate vaccines on d 0, 28, and 56. because hboc contains the crm197 mutant diphtheria toxin from corynebacterium diphtheriae as it ... | 1991 | 2000253 |
| elimination of the disulphide bridge in fragment b of diphtheria toxin: effect on membrane insertion, channel formation, and atp binding. | active diphtheria toxin consists of two disulphide-linked fragments, termed a and b. fragment b, which contains an internal disulphide bridge, facilitates translocation of the enzymatically active fragment a to the cytosol of eukaryotic cells. in this process cation-selective channels are formed. an in vitro translated full-length mutant lacking the internal disulphide bridge (a-58**) was functionally indistinguishable from its disulphide-containing counterpart (a-58) with respect to trypsin sen ... | 1991 | 1646374 |
| [the level of postvaccinal immunity to diphtheria, tetanus and poliomyelitis in relation to the number of inoculations]. | the work was aimed at the comparative study of the intensity of immunity to diphtheria, tetanus and poliomyelitis, depending on the number of injections of adsorbed diphtheria-pertussis-tetanus (dpt) vaccine and poliomyelitis vaccine, for the purpose of finding out the possibility of reducing the antigenic load given to children without diminishing the intensity of immunity. to determine the level of immunity to diphtheria and tetanus, 1900 children under school age were serologically studied by ... | 1991 | 1650072 |
| toxigenic corynebacterium diphtheriae associated with the haj. | 1991 | 1669870 | |
| routine screening for corynebacterium diphtheriae. | 1991 | 1678835 | |
| medical and microbiological problems arising from airborne infection in hospitals. | the practical importance and frequency of airborne nosocomial infections has been a matter of dispute for many years. this is because most of the pathogens acquired in hospitals are able to use various different routes of infecting the patient's body so that it may be difficult or even impossible to prove an individual infection to be airborne. only microbes such as streptococcus pyogenes, neisseria meningitidis, corynebacterium diphtheriae, mycobacterium tuberculosis, or certain respiratory vir ... | 1991 | 1679815 |
| characterization of a defective diphtheria toxin repressor (dtxr) allele and analysis of dtxr transcription in wild-type and mutant strains of corynebacterium diphtheriae. | the production of diphtheria toxin and siderophore by the corynebacterium diphtheriae regulatory mutant c7(beta)hm723 is resistant to the inhibitory effects of iron, and the mutant strain is defective for function of the regulatory gene dtxr. a 2.8-kb hindiii fragment carrying the c7(beta)hm723 dtxr allele was cloned and characterized in escherichia coli. the restriction endonuclease maps of the 2.8-kb hindiii fragment from c7(beta)hm723 and the corresponding fragment from wild-type c. diphtheri ... | 1991 | 1718867 |
| [a phase-i clinical trial of a new antitetanus/antidiphtheria vaccine for adults]. | 1991 | 1725595 | |
| toxigenic diphtheria in two isolated northern communities. | 1991 | 1365150 | |
| [the use of medium 199 in the bacteriological diagnosis of diphtheria]. | 1990 | 1691349 | |
| [a serum-free variant of a medium for determining the toxigenicity of corynebacterium diphtheriae]. | 1990 | 1699074 | |
| [a case of diphtheria in an adult]. | 1990 | 1704664 | |
| [the status of antidiphtheria immunity and of the toxigenic corynebacterium diphtheriae carrier state in organized collectives of adults]. | 1990 | 2077715 | |
| [classical pseudomembranous diphtheria caused by corynebacterium ulcerans]. | in a 63-year-old patient presenting with signs of classical diphtheria including membranous pharyngotracheobronchitis and myocarditis, corynebacterium ulcerans was isolated in pure culture from the membranes. the strain produced a toxin specific for c. ulcerans but no diphtheria toxin. the patient was discharged after a complicated hospital course including respiratory and cardiac failure. besides corynebacterium diphtheriae, which has very rarely been isolated in switzerland in recent years, co ... | 1990 | 2084836 |
| localization of an origin of replication in corynebacterium diphtheriae broad host range plasmid png2 that also functions in escherichia coli. | subcloning and protoplast transformation studies identified a 2.6 kb fragment of corynebacterium diphtheriae plasmid png2 which contains an origin of replication (orir). molecular combination of the 2.6 kb orir cartridge with escherichia coli plasmid puc18cmr enabled the e. coli cloning vector to replicate within several species of corynebacterium host cells. a 2.6 kb plasmid formed from the orir cartridge alone is capable of replicating in e. coli. this suggests that a single origin could be us ... | 1990 | 2108894 |
| dna sequence homology between attb-related sites of corynebacterium diphtheriae, corynebacterium ulcerans, corynebacterium glutamicum, and the attp site of gamma-corynephage. | chromosomal restriction fragments of corynebacterium ulcerans and c. diphtheriae, containing an integration site for corynephages of the beta family, show homology on southern blots. homologous dna in also found in the soil isolate c. glutamicum, although this strain is not susceptible to beta-corynephages. three of these dna fragments, one for each bacterial strain, and a fragment of gamma-corynephage dna previously shown to contain the phage integration site, were cloned and sequenced. alignme ... | 1990 | 2108899 |
| nucleotide sequence of the erythromycin resistance gene from the corynebacterium plasmid png2. | 1990 | 2110657 | |
| effect of low mole fraction of trehalose dicorynomycolate from corynebacterium diphtheriae on water permeability and electrical capacitance of lipid bilayer membranes. | the effect of incorporation of different proportions of trehalose dicorynomycolates (tdc) into lecithin bilayer membranes was studied. it was found that tdc, induces a 14% decrease of water osmotic permeability (42.6 +/- 3.9 to 36.8 +/- 2.7 microns/s) at 1.6 mole%, suggesting that this substance leads to an increase of the degree of packing of the constituent lipid molecules. a condensing effect of tdc was also apparent from membrane electrical capacitance (cm) measurement. by incorporating tdc ... | 1990 | 2111231 |
| [deoxyribonuclease from corynebacterium diphtheriae: dynamics of synthesis and properties]. | diphtheritic bacteria of pw-8 massachusetts strain produced into cultural medium only one nucleotidase--endodnaase. the enzyme was synthesized by the cells during the exponential phase of growth. the dnaase was purified 500-fold and exhibited properties specific to neutral-alkaline dnaases (ph optimum about 7.5, absolute requirements for me2+, single-step mechanism of substrate hydrolysis). the following properties were typical for the enzyme: absence of distinct specificity to structure of base ... | 1990 | 2111600 |
| [diagnosis of diphtheria]. | 1990 | 2113126 | |
| [corynebacteria of the jk group and their sensitivity to antibacterial drugs]. | 1990 | 2113801 | |
| molecular cloning and dna sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxr) from corynebacterium diphtheriae. | although the structural gene for diphtheria toxin, tox, is carried by a family of closely related corynebacteriophages, the regulation of tox expression is controlled, to a large extent, by its bacterial host corynebacterium diphtheriae. optimal yields of tox gene products are obtained only when iron becomes the growth-rate-limiting substrate. previous studies suggest that regulation of tox expression is mediated through an iron-binding aporepressor. to facilitate molecular cloning of the tox re ... | 1990 | 2116013 |
| acp broadsheet no 125: july 1990. bacteriological diagnosis of diphtheria. | 1990 | 2116457 | |
| [the immunological effectiveness of small doses of diphtheria and tetanus anatoxins in the revaccination of children with allergy-altered reactivity and adults]. | the possibility of decreasing the content of antigens in adsorbed diphtheria-tetanus toxoid, used for the booster immunization of children with allergically altered responsiveness and adults, is shown. diphtheria toxoid in doses of 1-2.5 lf and tetanus toxoid in doses of 1-2.5 binding units possessed sufficiently high immunological effectiveness in combination with low reactogenicity and good tolerance. in 6-12 months after booster immunization with decreased doses of toxoids 100% of children wi ... | 1990 | 2117319 |
| effect of different concentrations of diphtheria toxoid & aluminum phosphate on potency of diphtheria component in dpt vaccines. | an effort was made to determine the optimum concentration of diphtheria toxoid in combination with aluminum phosphate gel in dpt vaccines which may give a safe, potent and economical preparation. the effect of four different concentrations of aluminum phosphate and three different antigenic concentrations of diphtheria toxoid on potency of diphtheria component in dpt vaccine was assessed. a gradual increase in potency was seen with increase in toxoid concentration and a gradual decrease in poten ... | 1990 | 2118880 |
| [the cellular immune response in different forms of diphtheria in adults]. | in 109 adult diphtheria patients with different forms of the disease (toxic, subtoxic, localized) and carriers, the latter including 6 persons having had the localized form of diphtheria, 12 different subpopulations of t and b lymphocytes and neutrophils were studied by the methods of rosette formation with sheep, mouse and bovine red blood cells. the study showed that, starting from week 2 of the disease, an increase in the content of the subpopulations under study was registered in all forms o ... | 1990 | 2122622 |
| sandwich-dot immunobinding assay (sandwich-dia), a new immunological method for the detection of diphtheria toxin. | in this paper we describe a new assay for diphtheria toxin in bacterial cultures, based on a sandwich-dot immunobinding method. this method uses horse polyclonal diphtheria antitoxin as the coating antibody and mouse monoclonal diphtheria antitoxin as the detecting antibody. the sensitivity of this new method is within a range of 5-10 ng toxin per ml in corynebacterium diphtheriae cultures. we did not observe any false-positive reactions. as a solid phase we used nitrocellulose disks which bind ... | 1990 | 2124491 |
| [the immunomodulating properties of a diphtheria bacterial vaccine in in vitro experiments]. | in this investigation lymphocytes sensitized with corynebacterium diphtheriae antigens obtained from carriers and convalescents were used. the new diphtheria bacterial vaccine codivac, in contrast to other comparable preparations (diphtheria toxoid, staphylococcal toxoid, staphylococcal vaccine, levamisole), was found to produce a more direct effect by modulating the levels of t-lymphocytes, depending on their initial levels in the patient. codivac, together with other preparations, can be used ... | 1990 | 2129148 |
| coordinate regulation of siderophore and diphtheria toxin production by iron in corynebacterium diphtheriae. | iron is an environmental signal which regulates the coordinate expression of genes associated with virulence in many pathogenic bacteria. in response to iron-deprivation, lysogenic corynebacterium diphtheriae c7 (beta) synthesizes and secretes diphtheria toxin and siderophore and induces a high-affinity iron uptake system. diphtheria toxin is encoded by beta phage, but genes for siderophore production are encoded on the bacterial chromosome. diphtheria toxin and siderophore production were shown ... | 1990 | 2151460 |
| diphtheritic polyneuritis in an elderly woman: clinical and neurophysiological follow-up. | a 71-year-old english lady initially presented with a bulbar paralysis and, six weeks later, developed a generalised sensori-motor neuropathy. corynebacterium diphtheriae mitis was cultured from her throat swab. despite a good clinical recovery at one month, nerve conduction velocity was at its lowest. as far as the authors are aware, this is one of the few cases of neurophysiological and clinical follow-up in a british subject with diphtheritic peripheral neuropathy. this case emphasises the im ... | 1990 | 2169838 |
| detection of diphtheria toxin in culture supernates of corynebacterium diphtheriae and c. ulcerans by immunoassay with monoclonal antibody. | monoclonal antibody (mab) to diphtheria toxin was produced in mouse hybridomas, and shown by elisa to be of sub-class igg1. hybridomas were inoculated into mice to produce ascitic fluid from which mab was purified by caprylic acid. the mab was shown by immunoblotting to be directed against the a fragment of the toxin and also against the intact toxin molecule. after conjugation with fluorescein isothiocyanate, it was used in an immunoassay to detect toxin in culture supernates of corynebacterium ... | 1990 | 2202836 |
| [cloning and expression of the gene for diphtheria toxin and its subunits in escherichia coli]. | the results of cloning corynebacterium diphtheriae phi 984 tox gene and its a and b subunits in escherichia coli are presented. regulatory sequences of tox gene are capable to promote effective expression in e. coli cells. a set of recombinant plasmids has been obtained which can determine the synthesis of a and b individual subunits and are suitable for constructing immunotoxins by gene engineering. the diphtheria toxin of 62 kda synthesized in e. coli has enzymatic activity and reacts with ant ... | 1990 | 2227396 |
| nucleotide sequence of the replication region from the mycobacterium-escherichia coli shuttle vector pep2. | the replication region derived from the corynebacterium diphtheriae-escherichia coli plasmid png2 was sequenced. this 1.85-kb segment contains a single origin of dna replication, one major open reading frame and shares no sequence homology with previously described plasmids. | 1990 | 2265753 |
| [immunotoxins]. | an immunologic approach besides surgical, radiotherapeutic, chemotherapeutic methods in the treatment of cancer was proposed for the first time by paul ehrlich in 1906. the structure, produced by conjugating the antibodies against the tumor cells with a toxin molecule by moolten and cooperband (1970) was named as immunotoxins. producing antibodies with the desired amount and purity was achieved by improving the hybridoma technology, immunotoxins were tried to be produced by conjugating the monoc ... | 1990 | 2283968 |
| diphtheria with a difference--a rare corynebacterium fatality with associated apoptotic cell death. | a case of infection with corynebacterium ulcerans (c. ulcerans), resulted in the sudden death of a previously healthy 73-year-old woman. death from corynebacterium diphtheriae (c. diphtheriae) infection is well-documented. fatalities following infection with c. ulcerans are unreported; this would appear to be the first documented death due to such infection. | 1990 | 2323738 |