Publications

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[phage resistance of corynebacterium crenatum conferred by the restriction and modification system cgli].in order to prevent phage contamination in amino acid fermentation, we introduced the restriction and modification system cgli gene complex into corynebacterium crenatum and studied their phage-resistance. the cgli gene complex was amplified from corynebacterium glutamicum by pcr and constructed into pjl23 vector. the recombinant strains were obtained by transformation of the recombinant plasmid pjl23-cgli into c. crenatum. results showed that the recombinant strains possessed strong phage-resis ...200818724694
cloning and functional analysis of novel short-chain cis-prenyltransferases.cis-prenyltransferase catalyzes the synthesis of z,e-mixed prenyl diphosphates by sequential condensation of isopentenyl diphosphate with allylic diphosphate. cis-prenyltransferases can be classified into three subgroups: short-, medium-, and long-chain cis-prenyltransferase, according to their product chain lengths. cis-farnesyl diphosphate synthase from mycobacterium tuberculosis has been the only example as short-chain cis-prenyltransferase so far characterized. in this study, we cloned the n ...200818725204
evaluation of the humoral and cellular immune response to different antigens of corynebacterium pseudotuberculosis in canindé goats and their potential protection against caseous lymphadenitis.corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis, a disease that affects goats and sheep, and can cause severe economic losses. in this study, four different antigenic extracts were obtained from the attenuated strain t1, which was isolated in the state of bahia (brazil). forty-four canindé breed goats were divided in five groups, each receiving a different antigen solution and saline buffer as a control. the humoral response was monitored through the identifica ...200818752855
engineering of a glycerol utilization pathway for amino acid production by corynebacterium glutamicum.the amino acid-producing organism corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. by heterologous expression of escherichia coli glycerol utilization genes, c. glutamicum was engineered to grow on glycerol. while expression of the e. coli genes for glycerol kinase (glpk) and glycerol 3-phosphate dehydrogenase (glpd) was sufficient for growth on glycerol as the sole carbon and energy source, additional expression of the aquaglyceroporin gen ...200818757581
production of 2-methyl-1-butanol in engineered escherichia coli.recent progress has been made in the production of higher alcohols by harnessing the power of natural amino acid biosynthetic pathways. here, we describe the first strain of escherichia coli developed to produce the higher alcohol and potential new biofuel 2-methyl-1-butanol (2mb). to accomplish this, we explored the biodiversity of enzymes catalyzing key parts of the isoleucine biosynthetic pathway, finding that ahas ii (ilvgm) from salmonella typhimurium and threonine deaminase (ilva) from cor ...200818758769
heterologous ectoine production in escherichia coli: by-passing the metabolic bottle-neck.transcription of the ectoine biosynthesis genes ecta, ectb and ectc from marinococcus halophilus in recombinant escherichia coli dh5alpha is probably initiated from three individual sigma70/sigmaa-dependent promoter sequences, upstream of each gene. consequently, mrna-fragments containing the single genes and combinations of the genes ecta and ectb or ectb and ectc, respectively, could be detected by northern blot analysis. under the control of its own regulatory promoter region (ectup) a seemin ...200818759971
metabolic engineering for bioproduction of sugar alcohols.sugar alcohols find applications in pharmaceuticals, oral and personal care products, and as intermediates in chemical synthesis. while industrial-scale production of these compounds has generally involved catalytic hydrogenation of sugars, microbial-based processes receive increasing attention. the past few years have seen a variety of interesting metabolic engineering efforts to improve the capabilities of bacteria and yeasts to overproduce xylitol, mannitol, and sorbitol. examples include het ...200818760354
development of an online database for diphtheria molecular epidemiology under the remit of the dipnet project. 200818761980
an efficient succinic acid production process in a metabolically engineered corynebacterium glutamicum strain.a corynebacterium glutamicum strain (deltaldha-pcra717) that overexpresses the pyc gene encoding pyruvate carboxylase while simultaneously exhibiting a disrupted ldha gene encoding l-lactate dehydrogenase was investigated in detail for succinic acid production. succinic acid was shown to be efficiently produced at high-cell density under oxygen deprivation with intermittent addition of sodium bicarbonate and glucose. succinic acid concentration reached 1.24 m (146 g l(-1)) within 46 h. the yield ...200818777022
the molecular switch that activates the cell wall anchoring step of pilus assembly in gram-positive bacteria.cell surface pili in gram-positive bacteria orchestrate the colonization of host tissues, evasion of immunity, and the development of biofilms. recent work revealed that pilus assembly is a biphasic process wherein pilus polymerization is catalyzed by a pilus-specific sortase followed by cell wall anchoring of the pilus that is promoted by the housekeeping sortase. here, we present molecular genetic and biochemical studies of a heterotrimeric pilus in corynebacterium diphtheriae, uncovering the ...200818779588
enzyme-triggered and self-cleaving fragrant alcohol precursors.the high volatility and water solubility of many natural perfumery alcohols leads to their rapid loss in fabric-care and personal-care applications. a dramatically enhanced substantivity is achieved by the use of fragrance precursors as controlled-release systems. in the first part of this article, we present multi-odorant precursors, in which the enzymatic cleavage of esters or carbonates of fragrant alcohols triggers subsequent steps leading to the release of fragrant ketones, lactones, and ad ...200818618399
assessment of the use of anterior segment optical coherence tomography in microbial keratitis.to investigate the imaging capabilities of anterior segment optical coherence tomography (as oct) in microbial keratitis and to assess whether measurements of the quantitative parameters, infiltrate thickness and corneal thickness, were possible.200818602080
detection of toxigenic corynebacterium diphtheriae and corynebacterium ulcerans strains by a novel real-time pcr. 200818550743
a description of genes of corynebacterium pseudotuberculosis useful in diagnostics and vaccine applications.corynebacterium pseudotuberculosis, a gram-positive intracellular pathogen, is the etiological agent of caseous lymphadenitis or cla. this bacterium infects goats and sheep and causes great economic losses worldwide annually, mainly for goat producers. despite its importance, cla is still poorly characterized. however, with advances in the genomic field, many c. pseudotuberculosis genes have already been characterized, mainly those related to virulence such as phospholipase d. here, we examined ...200818551390
[development of growth medium from meat processing waste products and assessment of its properties].new growth medium based on inedible substrate - meat processing waste products of slaughterhouses - was developed. new medium was characterized by physical, chemical, and biological properties using exacting and non-exacting microorganisms, as well as by periodical cultivation of corynebacterium diphtheriae strain and obtaining the preparation of its antigens. the experimental medium provided the growth of chosen test-strains with typical properties. from biomass obtained during the periodic cul ...200818595471
characterization of cg10062 from corynebacterium glutamicum: implications for the evolution of cis-3-chloroacrylic acid dehalogenase activity in the tautomerase superfamily.a 149-amino acid protein designated cg10062 is encoded by a gene from corynebacterium glutamicum. the physiological function of cg10062 is unknown, and the gene encoding this protein has no obvious genomic context. sequence analysis links cg10062 to the cis-3-chloroacrylic acid dehalogenase ( cis-caad) family, one of the five known families of the tautomerase superfamily. the characterized tautomerase superfamily members have two distinctive characteristics: a beta-alpha-beta structure motif and ...200818598055
results of diagnostic ophthalmic testing in healthy guinea pigs.objective-to report values for tear production, central corneal touch threshold (ctt), and intraocular pressure (iop) in healthy guinea pigs and determine results of aerobic bacterial culture and cytologic examination of conjunctival swab specimens. design-cross-sectional study. animals-31 healthy guinea pigs (62 eyes) of various ages and breeds. procedures-tear production was measured by the phenol red thread tear test (prt) and schirmer tear test (stt) before and after topical anesthetic appli ...200818598151
genome sequence of the lytic bacteriophage p1201 from corynebacterium glutamicum nchu 87078: evolutionary relationships to phages from corynebacterineae.p1201 is a lytic corynephage of corynebacterium glutamicum nchu 87078. its genome consists of a linear double-stranded dna molecule of 70,579 base pairs, with 3'-protruding cohesive ends of ten nucleotides. we have identified 69 putative open reading frames, including three apparent genes (thymidylate synthase, terminase, and rnr alpha subunit genes) that are interrupted by an intein. protein-splicing activities of these inteins were demonstrated in escherichia coli. three structural proteins in ...200818599103
development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples.to efficiently prevent and treat bovine mastitis and minimize its effect on the dairy industry, a sensitive, rapid, and specific test is required for identifying the mastitis-causing pathogens. in this study, a biochip capable of detecting 7 common species of mastitis-causing pathogens, including corynebacterium bovis, mycoplasma bovis, staphylococcus aureus, and the streptococcus spp. s. agalactiae, s. bovis, s. dysgalactiae, and s. uberis, within 6 hr was developed. the technique is based on d ...200818599851
microbial contamination of the tzu-chi cord blood bank from 2005 to 2006.in total, 4502 units of cord blood (cb) were collected during a 2-year period from 2005 to 2006 by the buddhist tzu-chi stem cells center. the aim of this study was to analyze the incidence of microbial contamination and type of organism present in the cord blood. the clinical impact of microbial contamination on hematopoietic progenitor cell (hpc) grafts used for hpc transplantation is also discussed.200818581722
corynebacterium bovis line related septicemia: a case report and review of the literature.corynebacterium bovis, the causative agent of bovine mastitis, has rarely been described as a human pathogen since its description. we report a rare case of line related septicemia due to corynebacterium bovis.200818584551
identification of a gene encoding a transporter essential for utilization of c4 dicarboxylates in corynebacterium glutamicum.the corynebacterium glutamicum r genome contains a total of eight genes encoding proteins with sequence similarity to c4-dicarboxylate transporters identified from other bacteria. three of the genes encode proteins within the dicarboxylate/amino acid:cation symporter (daacs) family, another three encode proteins within the tripartite atp-independent periplasmic transporter family, and two encode proteins within the divalent anion:na+ symporter (dass) family. we observed that a mutant strain defi ...200818586971
corynebacterium pseudotuberculosis and corynebacterium renale isolated from two arabian oryx (oryx leucoryx). 200818587065
evolution of metal(loid) binding sites in transcriptional regulators.expression of the genes for resistance to heavy metals and metalloids is transcriptionally regulated by the toxic ions themselves. members of the arsr/smtb family of small metalloregulatory proteins respond to transition metals, heavy metals, and metalloids, including as(iii), sb(iii), cd(ii), pb(ii), zn(ii), co(ii), and ni(ii). these homodimeric repressors bind to dna in the absence of inducing metal(loid) ion and dissociate from the dna when inducer is bound. the regulatory sites are often thr ...200818591244
the cgl1281-encoding putative transporter of the cation diffusion facilitator family is responsible for alkali-tolerance in corynebacterium glutamicum.mutants of corynebacterium glutamicum that were unable to grow under mild alkaline ph conditions were isolated by mutagenesis. strain al-43 exhibiting the highest sensitivity to alkaline ph among the mutants was selected and used to clone a dna fragment that could complement the phenotype. sequencing and subcloning of the cloned 4.0-kb ecori dna fragment showed that the cgl1281 gene was responsible for the complementation. the deduced amino acid sequence of cgl1281 was found to show significant ...200818592219
[the corynebacterium urealyticum genome has been completely sequenced: a key advance to know the uropathogenicity and "life style" of this microorganism]. 200818592763
corynebacterium glutamicum contains 3-deoxy-d-arabino-heptulosonate 7-phosphate synthases that display novel biochemical features.3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthase (ec 2.5.1.54) catalyzes the first step of the shikimate pathway that finally leads to the biosynthesis of aromatic amino acids phenylalanine (phe), tryptophan (trp), and tyrosine (tyr). in corynebacterium glutamicum atcc 13032, two chromosomal genes, ncgl0950 (arof) and ncgl2098 (arog), were located that encode two putative dahp synthases. the deletion of ncgl2098 resulted in the loss of the ability of c. glutamicum res167 (a restrictio ...200818621870
detection of corynebacterium kutscheri in the faeces of subclinically infected mice.mice were infected experimentally and subclinically with corynebacterium kutscheri to recover the organism from mice faeces. the faeces were then cultured using selective furazolidone-nalidixic acid-colimycin agar. the number of c. kutscheri per gram of fresh faeces varied from mouse to mouse, but once established in the intestine, the organism was excreted in the faeces for at least five months. viable bacteria were detected in most of the faecal samples, including those stored in the animal ro ...200818625593
biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase.the cell wall mycolyl-arabinogalactan-peptidoglycan complex is essential in mycobacterial species, such as mycobacterium tuberculosis and is the target of several antitubercular drugs. for instance, ethambutol targets arabinogalactan biosynthesis through inhibition of the arabinofuranosyltransferases mt-emba and mt-embb. a bioinformatics approach identified putative integral membrane proteins, msmeg2785 in mycobacterium smegmatis, rv2673 in mycobacterium tuberculosis and ncgl1822 in corynebacter ...200818627460
evaluation of three methods for dna fingerprinting of corynebacterium pseudotuberculosis strains isolated from goats in poland.phenotypic approaches based on metabolic and biological characteristics of corynebacterium pseudotuberculosis have been limited due to insufficient discrimination between closely related isolates. in this paper we present performance and convenience of three molecular typing methods: box-pcr, random amplification of polymorphic dna (rapd) and amplification of dna fragments surrounding rare restriction site (adsrrs-fingerprinting) in genome analysis of these bacteria. among examined 61 strains th ...200818646397
inactivation of cg10062, a cis-3-chloroacrylic acid dehalogenase homologue in corynebacterium glutamicum, by (r)- and (s)-oxirane-2-carboxylate: analysis and implications.( r)- and ( s)-oxirane-2-carboxylate were determined to be active site-directed irreversible inhibitors of the cis-3-chloroacrylic acid dehalogenase ( cis-caad) homologue cg10062 found in corynebacterium glutamicum. kinetic analysis indicates that the ( r) enantiomer binds more tightly and is the more potent inhibitor, likely reflecting more favorable interactions with active site residues. pro-1 is the sole site of covalent modification by the ( r) and ( s) enantiomers. pro-1, arg-70, arg-73, a ...200818646866
characterization of the gut bacterial community in manduca sexta and effect of antibiotics on bacterial diversity and nematode reproduction.the tobacco hornworm, manduca sexta, is a model lepidopteran insect used to study the pathogenic and mutualistic phases of entomopathogenic nematodes (epns) and their bacterial symbionts. while intestinal microbial communities could potentially compete with the epn and its bacterial partner for nutrient resources of the insect, the microbial gut community had not been characterized previously. here, we show that the midgut of m. sexta raised on an artificial diet contained mostly gram-positive c ...200818647359
mycothiol biosynthesis is essential for ethionamide susceptibility in mycobacterium tuberculosis.spontaneous mutants of mycobacterium tuberculosis that were resistant to the anti-tuberculosis drugs ethionamide and isoniazid were isolated and found to map to msha, a gene encoding the first enzyme involved in the biosynthesis of mycothiol, a major low-molecular-weight thiol in m. tuberculosis. seven independent missense or frameshift mutations within msha were identified and characterized. precise null deletion mutations of the msha gene were generated by specialized transduction in three dif ...200818651841
identification and characterization of the dicarboxylate uptake system dcct in corynebacterium glutamicum.many bacteria can utilize c(4)-carboxylates as carbon and energy sources. however, corynebacterium glutamicum atcc 13032 is not able to use tricarboxylic acid cycle intermediates such as succinate, fumarate, and l-malate as sole carbon sources. upon prolonged incubation, spontaneous mutants which had gained the ability to grow on succinate, fumarate, and l-malate could be isolated. dna microarray analysis showed higher mrna levels of cg0277, which subsequently was named dcct, in the mutants than ...200818658264
first detection of corynebacterium ulcerans producing a diphtheria-like toxin in a case of human with pulmonary infection in the rio de janeiro metropolitan area, brazil.the frequency and severity of human infections associated with corynebacterium ulcerans appear to be increasing in different countries. here, we describe the first c. ulcerans strain producing a diphtheria-like toxin isolated from an elderly woman with a fatal pulmonary infection and a history of leg skin ulcers in the rio de janeiro metropolitan area.200818660996
partial redundancy in the synthesis of the d-arabinose incorporated in the cell wall arabinan of corynebacterineae.the major cell wall carbohydrate of corynebacterineae is arabinogalactan (ag), a branched polysaccharide that is essential for the physiology of these bacteria. decaprenylphosphoryl-d-arabinose (dpa), the lipid donor of d-arabinofuranosyl residues of ag, is synthesized through a series of unique biosynthetic steps, the last one being the epimerization of decaprenylphosphoryl-beta-d-ribose (dpr) into dpa, which is believed to proceed via a sequential oxidation-reduction mechanism. two proteins fr ...200818667564
culture-independent nested pcr method reveals high diversity of actinobacteria associated with the marine sponges hymeniacidon perleve and sponge sp.a culture-independent nested polymerase chain reaction (pcr) technique was used to investigate the diversity of actinobacteria communities associated with the sponges hymeniacidon perleve and sponge sp. the phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16s rrna sequencing of cloned dna fragments. a total of 196 positive clones were screened by restriction fragment length polymorphism (rflp) analysis; 48 unique operational taxonomic units (otus) were selected for ...200818670903
corynebacterium ilicis is typified by icmp 2608 =icpb ci144, arthrobacter ilicis is typified by dsm 20138 =atcc 14264 =ncppb 1228 and the two are not homotypic synonyms, and clarification of the authorship of these two species. opinion 87.the judicial commission rules that the name corynebacterium ilicis mandel et al. 1961 is represented by the type strain icmp 2608 =icpb ci144 and is reported to be a plant-pathogenic species. arthrobacter ilicis is represented by the type strain dsm 20138 =atcc 14264 =ncppb 1228 and is not a homotypic synonym of corynebacterium ilicis mandel et al. 1961, and is reported not to be a plant pathogen. the authorship is to be cited as arthrobacter ilicis collins et al. 1982 and typification and the d ...200818676493
formation and enhanced biocidal activity of water-dispersable organic nanoparticles.water-insoluble organic compounds are often used in aqueous environments in various pharmaceutical and consumer products. to overcome insolubility, the particles are dispersed in a medium during product formation, but large particles that are formed may affect product performance and safety. many techniques have been used to produce nanodispersions-dispersions with nanometre-scale dimensions-that have properties similar to solutions. however, making nanodispersions requires complex processing, a ...200818685640
nitrogen control in mycobacterium smegmatis: nitrogen-dependent expression of ammonium transport and assimilation proteins depends on the ompr-type regulator glnr.the effect of nitrogen regulation on the level of transcriptional control has been investigated in a variety of bacteria, such as bacillus subtilis, corynebacterium glutamicum, escherichia coli, and streptomyces coelicolor; however, until now there have been no data for mycobacteria. in this study, we found that the ompr-type regulator protein glnr controls nitrogen-dependent transcription regulation in mycobacterium smegmatis. based on rna hybridization experiments with a wild-type strain and a ...200818689485
distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in corynebacterium glutamicum.corynebacterium glutamicum is a biotin auxotrophic bacterium in which glutamate production is induced under biotin-limited conditions. during glutamate production, anaplerotic reactions catalyzed by phosphoenolpyruvate carboxylase (pepc) and a biotin-containing enzyme pyruvate carboxylase (pc) are believed to play an important role in supplying oxaloacetate in the tricarboxylic acid cycle. to understand the distinct roles of pepc and pc on glutamate production by c. glutamicum, we observed gluta ...200818691531
characterization of developmental colony formation in corynebacterium glutamicum.we report that corynebacterium glutamicum colonies exhibit a developmental transition in culture. when cultured on a routinely used complete medium (cm2b), this bacterium first formed a flat translucent colony. subsequently, some parts of this colony expanded to form small spherical yellow colonies that finally developed into a single large yellow colony. the small flat colony consisted of long thick cells, which were occasionally v or y shaped, while the large yellow colony consisted of short s ...200818696061
[case of the month; purple urine bag syndrome (pubs)]. 200818460735
disruption of metf increased l-lysine production by methylophilus methylotrophus from methanol.methionine auxotrophic mutants of methylophilus methylotrophus as1 expressing a mutant form of dapa (dapa24) encoding a dihydrodipicolinate synthase desensitized from feedback inhibition by l-lysine, and mutated lyse (lyse24) encoding the l-lysine exporter from corynebacterium glutamicum 2256, produced higher amounts of l-lysine from methanol as sole carbon source than did other amino acid auxotrophic mutants. especially, the m. methylotrophus 102 strain, carrying both dapa24 and lyse24, produce ...200818460806
a putative alpha-helical porin from corynebacterium glutamicum.the cell wall of corynebacterium glutamicum contains a mycolic acid layer, which is a protective nonpolar barrier similar to the outer membrane of gram-negative bacteria. the exchange of material across this barrier requires porins. porin b (porb) is one of them. recombinant porb has been produced in escherichia coli, purified, crystallized and analyzed by x-ray diffraction, yielding 16 independent molecular structures in four different crystal forms at resolutions up to 1.8 a. all 16 molecules ...200818462756
antimicrobial and antioxidative activities of bioactive constituents from hydnophytum formicarum jack.hydnophytum formicarum jack. (rubiaceae) is a medicinal plant whose tubers possesses cardiovascular, anti-inflammatory and antiparasitic effects and have been used for the treatment of hepatitis, rheumatism and diarrhea. herein we report the isolation of its active constituents and the testing of their antimicrobial activity against 27 strains of microorganisms using an agar dilution method and of their antioxidative activity using the dpph and sod assays. the results show that the crude hexane, ...200818463592
encrusted cystitis by corynebacterium urealyticum: a case report with novel insights into bladder lesions. 200818467375
construction of heat-inducible expression vector of corynebacterium glutamicum and c. ammoniagenes: fusion of lambda operator with promoters isolated from c. ammoniagenes.the heat-inducible expression vectors for corynebacterium glutamicum and c. ammoniagenes were constructed by using the lambdaol1 and the cryptic promoters, cj1 and cj4 that express genes constitutively in c. ammoniagenes.. although the promoters were isolated from c. ammoniagenes, cj1 and cj4 were also active in c. glutamicum. to construct vectors, the ol1 from the lambdapl promoter was isolated and fused to the cj1 and cj4 promoters by recombinant pcr. the resulting artificial promoters, cj1o a ...200818467855
effect of increased glutamate availability on l-ornithine production in corynebacterium glutamicum.glutamate availability in the argf-argr-probdelta strain of corynebacterium glutamicum was increased by addition of glutamate to the cell or inactivation of the phosphoenolpyruvate carboxykinase activity and simultaneous overexpression of the pyruvate carboxylase activity to assess its effect on lornithine production. when glutamate was increased in an lornithine- producing strain, the production of l-ornithine was not changed. this unexpected result indicated that the intracellular concentratio ...200818467864
a randomized trial of 2% chlorhexidine tincture compared with 10% aqueous povidone-iodine for venipuncture site disinfection: effects on blood culture contamination rates.contaminated blood cultures have been recognized as a bothersome issue, and continue to cause frustration for clinicians. skin antiseptics can prevent blood culture contamination. to our knowledge, there have been no randomized studies to compare 2% alcoholic chlorhexidine and 10% aqueous povidone-iodine for venipuncture site disinfection.200818407355
the in-vitro antimicrobial activity of abrus precatorius (l) fabaceae extract on some clinical pathogens.to investigate the in-vitro antimicrobial activities of the extracts of abrus precatorius on some clinical isolates as resistance to available and affordable antibiotics by these pathogens is on the increase.200818408781
additions of killed whole cell bacteria preparations to freund complete adjuvant alter laying hen antibody response to soluble protein antigen.passive transfer of antibodies from hen to egg has value to both the producer of commercial polyclonal egg antibody and the producer of hatching eggs. water-in-oil emulsions are commonly amended with immune stimulants such as mycobacteria (e.g., freund complete adjuvant; fca) to increase antibody production to soluble protein antigens (spa). recent discoveries of the mechanisms by which microbial products act as adjuvants led us to hypothesize that additions of killed whole cell bacteria (bacter ...200818420981
structural characterization and functional properties of a novel lipomannan variant isolated from a corynebacterium glutamicum pimb' mutant.the genus corynebacterium is part of the phylogenetic group nocardioform actinomycetes, which also includes the genus mycobacterium. members of this phylogenetic group have a characteristic cell envelope structure, which is dominated by complex lipids and amongst these, lipoglycans are of particular interest. the disruption of ncgl2106 in c. glutamicum resulted in a mutant devoid of monoacylated phosphatidyl-myo-inositol dimannoside (ac(1)pim(2)) resulting in the accumulation of ac(1)pim(1) and ...200818421567
oxidative stress in leucocytospermic prostatitis patients: preliminary results.the aim of this study was to contribute to the knowledge concerning pathogenesis of inflammatory chronic prostatitis by revealing possible shifts in the balance of markers of oxidative stress and anti-oxidative activity in case of leucocytospermic prostatitis. we also attempted to identify possible relations between seminal micro-organisms and oxidative stress parameters. a many-sided complex of local (spermatozoa, seminal plasma) and general (blood, urine) markers in 21 prostatitis patients and ...200818477203
isolation of nitrate-reductase positive corynebacterium pseudotuberculosis from dromedary camels. 200818484117
a universal threshold concept for hydrophobic mycolata in activated sludge foaming.recent studies using quantitative fluorescence in situ hybridization (fish) have supported the principle that there are mycolata concentration thresholds, above which foaming is likely to occur. in this study, we surveyed 14 wastewater treatment plants (wwtps) in the uk, using quantitative fish, to establish that the principle of a mycolata threshold (2 x 10(6) mycolata cells ml(-1) mixed liquor suspended solids) is an empirical though widely held value. in addition, we designed, optimized and a ...200818486181
population heterogeneity in corynebacterium glutamicum atcc 13032 caused by prophage cgp3.the genome of corynebacterium glutamicum type strain atcc 13032 (accession number bx927147) contains three prophages, cgp1, cgp2, and cgp3. we recently observed that many genes within the cgp3 prophage region have increased mrna levels in a dtxr deletion mutant that lacks the master regulator of iron homeostasis (j. wennerhold and m. bott, j. bacteriol. 188:2907-2918, 2006). here, we provide evidence that this effect is due to the increased induction of the prophage cgp3 in the dtxr mutant, poss ...200818487330
comprehensive study of corynebacterium freneyi strains and extended and emended description of corynebacterium freneyi renaud, aubel, riegel, meugnier, and bollet 2001.in 2001, corynebacterium freneyi was described as a new fermentative, alpha-glucosidase-positive corynebacterium species related to c. xerosis based on data from three strains. during a review of our extensive culture collection we encountered 18 additional c. freneyi strains and further characterized them in detail. thirteen of the 18 strains were isolated from female genital tract specimens without any obvious disease association. phenotypically, c. freneyi can be easily differentiated from c. ...200818077628
high-level fluoroquinolone resistance in ophthalmic clinical isolates belonging to the species corynebacterium macginleyi.the clinical importance of nondiphtherial corynebacterium, a ubiquitous member of the normal human microflora of the skin and mucous membrane, for ocular surface infections has been recognized recently. we performed an antimicrobial susceptibility test with etest strips for three fluoroquinolones (ciprofloxacin, norfloxacin, and levofloxacin) and a taxonomic analysis on 21 isolates of corynebacterium from ophthalmic samples. of these, 16 isolates were identified as c. macginleyi at the species l ...200818077650
regulation of nitrogen metabolism in mycobacterium tuberculosis: a comparison with mechanisms in corynebacterium glutamicum and streptomyces coelicolor.the mechanisms governing the regulation of nitrogen metabolism in corynebacterium glutamicum and streptomyces coelicolor have been extensively studied. these actinomycetales are closely related to the mycobacterium genus and may therefore serve as a models to elucidate the cascade of nitrogen signalling in other mycobacteria. some factors involved in nitrogen metabolism in mycobacterium tuberculosis have been described, including glutamine synthetase and its adenylyltransferase, but not much dat ...200818493948
impact of pulsed electric fields on corynebacterium glutamicum cell membrane permeabilization.the permeability barrier of the microbial cell envelope for substrates and products often causes very low reaction rates of whole cells. therefore, it is of interest to develop an effective method to reduce this permeability barrier in order to increase product yields. utilisation of pulse electric fields may improve amino acid release from corynebacterium glutamicum by up to several orders of magnitude. in particular pulsed electric fields may change the cell/membrane's dielectric properties an ...200818499054
the significance of t cells, b cells, antibodies and macrophages against encephalomyocarditis (emc)-d virus-induced diabetes in mice.in order to clarify the significance of protective mechanisms against encephalomyocarditis (emc) virus-induced diabetes in mice, we studied the relative importance of t cells, b cells, antibodies and macrophages in the prevention of virus-induced diabetes. neither t cell-deficient athymic nude mice nor b cell-deficient micromt/micromt mice showed an enhanced clinical course of emc-d virus-induced diabetes, indicating that neither t cells nor b cells played a major role in the protection against ...200818500429
bacterial detection in apheresis platelets: blood systems experience with a two-bottle and one-bottle culture system.united blood services (ubs) began bacterial testing of platelets (plts) using one-bottle cultures in september 2003. collection of 7-day plts using two-bottle cultures began in april 2006. this study compares our experience using both systems.200818503612
the sequential action of a dipeptidase and a beta-lyase is required for the release of the human body odorant 3-methyl-3-sulfanylhexan-1-ol from a secreted cys-gly-(s) conjugate by corynebacteria.human axillary odor is formed by the action of corynebacteria on odorless axilla secretions. sulfanylalkanols, 3-methyl-3-sulfanylhexan-1-ol in particular, form one key class of the odoriferous compounds. a conjugate with the dipeptide cys-gly has been reported as the secreted precursor for 3-methyl-3-sulfanylhexan-1-ol. here, we confirm the cys-gly-(s) conjugate as the major precursor of this odorant, with lower levels of the cys-(s) conjugate being present in axilla secretions. the enzymatic r ...200818515361
sequencing and assembly of highly heterozygous genome of vitis vinifera l. cv pinot noir: problems and solutions.a new approach to sequencing and assembling a highly heterozygous genome, that of grape, species vitis vinifera cv pinot noir, is described. the combining of genome shotgun of paired reads produced by sanger sequencing and sequencing by synthesis of unpaired reads was shown to be an efficient procedure for decoding a complex genome. about 2 million snps and more than a million heterozygous gaps have been identified in the 500 mb genome of grape. more than 91% of the sequence assembled into 58,61 ...200818538432
molecular mass sorting of proteome using hollow fiber flow field-flow fractionation for proteomics.hollow fiber flow field-flow fractionation (hf flfff) has been demonstrated as a tool for pre-fractionating proteomes by differences in molecular mass (mr), where the resulting protein fractions are subsequently digested and analyzed by shotgun proteomics using two-dimensional liquid chromatography-electrospray ionization-tandem mass spectrometry (2d-lc-esi-ms/ms). hf flfff is a separation device capable of fractionating proteins or cells by hydrodynamic radius, and protein fraction can be readi ...200818541480
[interdigital erythrasma: clinical, epidemiologic, and microbiologic findings].erythrasma is a superficial infection caused by corynebacterium minutissimum and affects the major skin folds and the interdigital regions of the feet. it is characterized by erythematous, brown, scaly patches and maceration, and exhibits coral-red fluorescence under wood light.200818558055
microbiological and clinical features of corynebacterium urealyticum: urinary tract stones and genomics as the rosetta stone.corynebacterium urealyticum, formerly known as coryneform cdc group d2, was first recognized to be involved in human infections 30 years ago. it is a slow-growing, lipophilic, asaccharolytic and usually multidrug-resistant organism with potent urease activity. its cell wall peptidoglycan, menaquinone, mycolic and cellular fatty acid composition is consistent with that of the genus corynebacterium. dna-dna hybridization studies and 16s rdna sequencing analysis have been used to determine the degr ...200818558935
insights into the structural basis of substrate recognition by histidinol-phosphate aminotransferase from corynebacterium glutamicum.histidinol-phosphate aminotransferase (hisc) is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the reversible transamination reaction between histidinol phosphate (his-p) and 2-oxoglutarate (o-glu). the crystal structures of apo histidinol-phosphate aminotransferase from corynebacterium glutamicum, of the internal plp aldimine adduct and of a pyridoxamine 5-phosphate-enzyme complex were determined at resolutions of 2.2, 2.1 and 1.8 a, respectively. residues important for substrate spec ...200818560156
multicenter evaluation of the vitek 2 anaerobe and corynebacterium identification card.the new anaerobe and corynebacterium (anc) identification card for vitek 2 was compared with a 16s rrna gene sequencing (16s) reference method for accuracy in the identification of corynebacteria and anaerobic species. testing was performed on a vitek 2 xl system with modified software at three clinical trial laboratories. reproducibility was determined with nine atcc quality control strains that were tested 20 times over a minimum of 10 days at all three sites. a challenge set of 50 well-charac ...200818562580
pitfalls with diphtheria-like illness due to toxigenic corynebacterium ulcerans. 200818564932
analysis of 13c labeling enrichment in microbial culture applying metabolic tracer experiments using gas chromatography-combustion-isotope ratio mass spectrometry.the applicability of gas chromatography-combustion-isotope ratio mass spectrometry (gc-c-irms) for the quantification of 13c enrichment of proteinogenic amino acids in metabolic tracer experiments was evaluated. measurement of the 13c enrichment of proteinogenic amino acids from cell hydrolyzates of corynebacterium glutamicum growing on different mixtures containing between 0.5 and 10% [1-13c]glucose shows the significance of kinetic isotope effects in metabolic flux studies at low degree of lab ...200818565321
first clinical case of corynebacterium auriscanis isolated from localized dog bite infection.corynebacterium auriscanis has only previously been isolated from dogs and to our knowledge no cases of zoonotic transmission to humans have been reported. a case of a leg wound infection following a dog bite in a previously healthy human patient is described and confirms this organism to be a potential human pathogen.200818566151
1.6 angstroms structure of an nad+-dependent quinate dehydrogenase from corynebacterium glutamicum.to date, three different functional classes of bacterial shikimate/quinate dehydrogenases have been identified and are referred to as aroe, sdh-l and ydib. the enzyme aroe and the catalytically much slower sdh-l clearly prefer nadp+/nadph as the cosubstrate and are specific for (dehydro-)shikimate, whereas in ydib the differences in affinity for nadp+/nadph versus nad+/nadh as well as for (dehydro-)shikimate versus (dehydro-)quinate are marginal. these three subclasses have a similar three-dimen ...200818566515
direct visualization of the outer membrane of mycobacteria and corynebacteria in their native state.the cell envelope of mycobacteria, which include the causative agents of tuberculosis and leprosy, is crucial for their success as pathogens. despite a continued strong emphasis on identifying the multiple chemical components of this envelope, it has proven difficult to combine its components into a comprehensive structural model, primarily because the available ultrastructural data rely on conventional electron microscopy embedding and sectioning, which are known to induce artifacts. the existe ...200818567661
group 2 sigma factor sigb of corynebacterium glutamicum positively regulates glucose metabolism under conditions of oxygen deprivation.the sigb gene of corynebacterium glutamicum encodes a group 2 sigma factor of rna polymerase. under conditions of oxygen deprivation, the sigb gene is upregulated and cells exhibit high productivity of organic acids as a result of an elevated glucose consumption rate. using dna microarray and quantitative reverse transcription-pcr (rt-pcr) analyses, we found that sigb disruption led to reduced transcript levels of genes involved in the metabolism of glucose into organic acids. this in turn resul ...200818567683
the glxr regulon of the amino acid producer corynebacterium glutamicum: in silico and in vitro detection of dna binding sites of a global transcription regulator.the glxr (cg0350) gene of corynebacterium glutamicum atcc 13032 encodes a dna-binding transcription regulator of the crp/fnr protein family. five genomic dna regions known to be bound by glxr provided the seed information for dna binding site discovery by expectation maximization and gibbs sampling approaches. the detection of additional motifs in the genome sequence of c. glutamicum was performed with a position weight matrix and a profile hidden markov model, both deduced from the initial moti ...200818573287
novel lipoarabinomannan-like lipoglycan (cdilam) contributes to the adherence of corynebacterium diphtheriae to epithelial cells.the genus corynebacterium is part of the phylogenetic group nocardioform actinomycetes. members of this group have a characteristic cell envelope structure composed primarily of branched long-chain lipids, termed mycolic acids, and a rich number of lipoglycans such as lipoarabinomanans (lam) and lipomannans. in this study, we identified a novel lam variant isolated from corynebacterium diphtheriae named cdilam. the key structural features of cdilam are a linear alpha-1-->6-mannan with side chain ...200818575847
competition of reactive red 4, reactive orange 16 and basic blue 3 during biosorption of reactive blue 4 by polysulfone-immobilized corynebacterium glutamicum.competition of reactive red 4 (rr4), reactive orange 16 (ro16) and basic blue 3 (bb3) during biosorption of reactive blue 4 (rb4) by polysulfone-immobilized protonated corynebacterium glutamicum (pipc) was investigated in batch and column mode of operations. through potentiometric titrations, and with the aid of proton-binding model, carboxyl, phosphonate and amine were identified as functional groups of pipc, with apparent pk(a) values of 3.47+/-0.05, 7.08+/-0.07 and 9.90+/-0.05 mmol/g, respect ...200817913354
safety assessment of dairy microorganisms: aerobic coryneform bacteria isolated from the surface of smear-ripened cheeses.the group of "coryneform bacteria" belongs to the class of actinobacteria including a diverse and heterogeneous collection of bacteria of various genera. most of them are known as environmental residents and/or commensal flora of humans and they are isolated frequently in clinical studies. actinobacteria include also several aerobic species, present at the surface of smear-ripened cheeses for decades and used as ripening culture in the dairy industry. their clinical significance is controversial ...200817884218
engineering of an l-arabinose metabolic pathway in corynebacterium glutamicum.corynebacterium glutamicum was metabolically engineered to broaden its substrate utilization range to include the pentose sugar l-arabinose, a product of the degradation of lignocellulosic biomass. the resultant cra1 recombinant strain expressed the escherichia coli genes araa, arab, and arad encoding l-arabinose isomerase, l-ribulokinase, and l-ribulose-5-phosphate 4-epimerase, respectively, under the control of a constitutive promoter. unlike the wild-type strain, cra1 was able to grow on mine ...200817965859
characterization of mucosal biofilms on human adenoid tissues.to demonstrate the presence of mucosal biofilm in adenoid tissue using double staining for visualization of both the bacterial matrix and the bacterial cells. to identify bacterial species present on the surface of the studied adenoids.200817975509
analysis of toxigenic corynebacterium ulcerans strains revealing potential for false-negative real-time pcr results.diphtheria surveillance depends on the rapid and reliable recognition of the toxin gene in corynebacterium diphtheriae. real-time pcr is a rapid tool to confirm the presence of the diphtheria toxin gene (tox) in an isolate or specimen. we report that some toxigenic corynebacterium ulcerans strains show atypical results in a real-time pcr for tox.200817989189
the isolation of corynebacterium coyleae from clinical samples: clinical and microbiological data.fourteen corynebacterium coyleae isolates were recovered from 12 in-patients during a 5-years period. in six patients, the isolates were considered as clinically significant, three definite (sepsis), two probable (sepsis and soft tissue infection), and one possible (post-transfusional bacteremia). in the remaining 6 patients (all neonatal bacteremias), there was not enough data for considering the isolates as clinical significant. api coryne identified all isolates as c. jeikeium, while biolog g ...200817992547
septicemia caused by corynebacterium macginleyi: a rare form of extraocular infection. 200817981483
microbial interactions within a cheese microbial community.the interactions that occur during the ripening of smear cheeses are not well understood. yeast-yeast interactions and yeast-bacterium interactions were investigated within a microbial community composed of three yeasts and six bacteria found in cheese. the growth dynamics of this community was precisely described during the ripening of a model cheese, and the lotka-volterra model was used to evaluate species interactions. subsequently, the effects on ecosystem functioning of yeast omissions in ...200817981942
cloning, overexpression, purification, and characterization of s-adenosylhomocysteine hydrolase from corynebacterium efficiens ys-314.the gene encoding s-adenosylhomocysteine hydrolase activity (sahase: ec 3.3.1.1) from corynebacterium efficiens (ys-314) was cloned and expressed as a fusion protein in escherichia coli rosetta (de3). the analyzed nucleotide sequence of the cloned gene proved to be identical to those reported on the ncbi database. the recombinant enzyme is a tetramer, showing a molecular weight of approximately 210 kda, as estimated by gel filtration. the k(m) values of the enzyme for s-adenosylhomocysteine (sah ...200818034499
corynebacterium minutissimum bacteremia and meningitis: a case report and review of literature.corynebacterium minutissimum, the causative agent of erythrasma, is a gram-positive, non-spore forming, aerobic or facultative anaerobic bacillus. it has rarely been associated with extracutaneous disease, since its description in 1961. a computerized medline search for review of literature was performed. to our knowledge, there have been 18 cases of c. minutissimum infections that caused conditions other than erythrasma. these include reports of cases of abscess formation, intravascular cathete ...200818036665
characterization of mutations induced by n-methyl-n'-nitro-n-nitrosoguanidine in an industrial corynebacterium glutamicum strain.mutations induced by classical whole-cell mutagenesis using n-methyl-n'-nitro-n-nitrosoguanidine (ntg) were determined for all genes of pathways from glucose to l-lysine in an industrial l-lysine producer of corynebacterium glutamicum. a total of 50 mutations with a genome-wide distribution were identified and characterized for mutational types and mutagenic specificities. those mutations were all point mutations with single-base substitutions and no deletions, frame shifts, and insertions were ...200818037338
regulation of l-lactate utilization by the fadr-type regulator lldr of corynebacterium glutamicum.corynebacterium glutamicum can grow on l-lactate as a sole carbon and energy source. the ncgl2816-lldd operon encoding a putative transporter (ncgl2816) and a quinone-dependent l-lactate dehydrogenase (lldd) is required for l-lactate utilization. dna affinity chromatography revealed that the fadr-type regulator lldr (encoded by ncgl2814) binds to the upstream region of ncgl2816-lldd. overexpression of lldr resulted in strongly reduced ncgl2816-lldd mrna levels and strongly reduced lldd activity, ...200818039772
microbial production of l -glutamate and l -glutamine by recombinant corynebacterium glutamicum harboring vitreoscilla hemoglobin gene vgb.vitreoscilla hemoglobin (vhb) gene vgb equipped with a native promoter pvgb or a tac promoter ptac was introduced into corynebacterium glutamicum atcc14067, respectively. ptac was proven to be more suitable for expressing vhb protein in higher concentration in both escherichia coli and c. glutamicum strains compared with the native vgb promoter pvgb. vhb-expressing c. glutamicum exhibited higher oxygen uptake rate and enhanced cell growth. recombinant c. glutamicum harboring vgb gene equipped wi ...200818040683
co-ordinated regulation of gluconate catabolism and glucose uptake in corynebacterium glutamicum by two functionally equivalent transcriptional regulators, gntr1 and gntr2.corynebacterium glutamicum is a gram-positive soil bacterium that prefers the simultaneous catabolism of different carbon sources rather than their sequential utilization. this type of metabolism requires an adaptation of the utilization rates to the overall metabolic capacity. here we show how two functionally redundant gntr-type transcriptional regulators, designated gntr1 and gntr2, co-ordinately regulate gluconate catabolism and glucose uptake. gntr1 and gntr2 strongly repress the genes enco ...200818047570
performance of a whole blood interferon-gamma assay for detection and eradication of caseous lymphadenitis in sheep.caseous lymphadenitis (cla), a chronic bacterial disease of sheep and goats caused by corynebacterium pseudotuberculosis, could be controlled by eradication of infected carriers. this study aimed at validation of a whole blood interferon-gamma (ifn-gamma) enzyme immunoassay (eia) (bovigam, pfizer) in naturally infected sheep for use in eradication of infection from a flock. this assay used formalin-inactivated whole bacterial cells as antigen. the sensitivity of the whole cell assay was improved ...200818054178
a case of septic shock secondary to corynebacterium aquaticum bacteremia occurring in an hiv-infected patient attending a promiscuous thermal spa.corynebacterium aquaticum, an environmental organism associated with fresh water, has very seldom proved to be a cause of infection, although it has increasingly been isolated from clinical specimens. this report describes an unusual case of bacteremia occurring in an hiv-infected patient, complicated by septic shock and secondary to an epididimo-orchitis. combination therapy of levofloxacin and metronidazole was used successfully.200818059254
a new approach to study the decolorization of complex reactive dye bath effluent by biosorption technique.this work focused on the development of a practical biosorbent for the decolorization of textile effluents. the fermentation waste, corynebacterium glutamicum biomass, when decarboxylated and immobilized in polysulfone matrix performed well in decolorization of simulated reactive dye bath effluent comprised of four different reactive dyes and other auxiliary chemicals. the regeneration of polysulfone-immobilized c. glutamicum was successful with the aid of 0.01 m naoh as the eluant, which enable ...200818060770
[encrusted cystitis due to corynebacterium urealyticum].we reported the case of a 61 year-old man who presented an encrusted cystitis by corynebacterium urealyticum associated with bilateral hydronephrosis four months after adenomectomy complicated by vesicocutaneous fistula. medical treatment by quinolones and oral acidification of the urine allowed regression of the encrusted calcifications and resolution of the hydronephrosis.200818063228
production of chryseobacterium proteolyticum protein-glutaminase using the twin-arginine translocation pathway in corynebacterium glutamicum.the protein glutaminase (pg) secreted by the gram-negative bacterium chryseobacterium proteolyticum can deamidate glutaminyl residues in several substrate proteins, including insoluble wheat glutens. this enzyme therefore has potential application in the food industry. we assessed the possibility to produce pg containing a pro-domain in corynebacterium glutamicum which we have successfully used for production of several kinds of proteins at industrial-scale. when it was targeted to the general p ...200818064454
genetic and biochemical characterization of a 4-hydroxybenzoate hydroxylase from corynebacterium glutamicum.corynebacterium glutamicum uses 4-hydroxybenzoic acid (4hba) as sole carbon source for growth. previous studies showed that 4hba was taken up into cells via pcak, and the aromatic ring was cleaved via protocatechuate 3,4-dioxygenase. in this study, the gene poba ( cg ) (ncgl1032) involved in the conversion of 4hba into 3,4-dihydroxybenzoate (protocatechuate) was identified, and the gene product poba (cg) was characterized as a 4hba 3-hydroxylase, which is a homodimer of poba(cg). the poba (cg) i ...200818071645
parameter estimation and simulations of a mathematical model of corynebacterium pseudotuberculosis transmission in sheep.caseous lymphadenitis (cla) is an infectious disease of sheep caused by corynebacterium pseudotuberculosis. it is prevalent in most sheep producing countries and was introduced into the uk sheep population in 1991. the pathogen invades the host through epithelium and forms an abscess in the local draining lymph node. typically, disease presents as clinical, with overt (externally visible) swollen lymph nodes (the parotid, submandibular, prefemoral, prescapular, popliteal or mammary) or sub-clini ...200818164500
detection of bacteria in placental tissues obtained from extremely low gestational age neonates.the objective of the study was to quantify and identify aerobic and anaerobic bacteria as well as mycoplasma and ureaplasma in the chorionic parenchyma.200818166321
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