Publications

deinococcus radiodurans dna increases the radiation resistance of escherichia coli.	a genomic dna library of deinococcus radiodurans dna has been prepared using the plasmid vector pbr322. the recombinant plasmid was used to transform a more radiation-sensitive organism, escherichia coli rr1. following selection of transformed organisms by their ability to grow on ampicillin, radiation-resistant organisms were selected by irradiation with 137cs gamma radiation. increased radiation resistance correlates with the presence of a 3-kb fragment of dna in these cells which is derived f ...	1989	2687934
drdi, a unique restriction endonuclease from deinococcus radiodurans which recognizes 5'gacn6gtc3'.		1989	2542897
the deinococcus-thermus phylum and the effect of rrna composition on phylogenetic tree construction.	through comparative analysis of 16s ribosomal rna sequences, it can be shown that two seemingly dissimilar types of eubacteria deinococcus and the ubiquitous hot spring organism thermus are distantly but specifically related to one another. this confirms an earlier report based upon 16s rrna oligonucleotide cataloging studies (hensel et al., 1986). their two lineages form a distinctive grouping within the eubacteria that deserved the taxonomic status of a phylum. the (partial) sequence of t. ...	1989	11542160
characterization of 15 selected coccal bacteria isolated from antarctic rock and soil samples from the mcmurdo-dry valleys (south-victoria land).	approximately 1500 cultures of microorganisms were isolated from rocks and soils of the ross desert (mcmurdo-dry valleys). from these, 15 coccoid strains were chosen for more detailed investigation. they were characterized by morphological, physiological and chemotaxonomical properties. all isolates were gram-positive, catalase-positive and nonmotile. six strains showed red pigmentation and could be identified as members of the genera micrococcus (m. roseus, m. agilis) or deinococcus. in sp ...	1988	11538335
duplication insertion of drug resistance determinants in the radioresistant bacterium deinococcus radiodurans.	escherichia coli drug resistance plasmids were introduced into deinococcus radiodurans by cloning d. radiodurans dna into the plasmids prior to transformation. the plasmids were integrated into the chromosome of the transformants and flanked by a direct repeat of the cloned d. radiodurans segment. the plasmid and one copy of the flanking chromosomal segment constituted a unit ("amplification unit") which was found repeated in tandem at the site of chromosomal integration. up to 50 copies of the ...	1988	2834326
isolation of a dna-binding protein from deinococcus radiodurans having an affinity for a z-form polynucleotide.	a protein which preferentially binds z-form duplex dna has been purified from the cells of deinococcus radiodurans. the molecular weight of the protein was estimated to be approximately 68,000 by gel filtration and sds-polyacrylamide gel electrophoresis. amino acid analysis of the protein indicates that it is not so basic since it contains a lower mole percent of lysine and higher mole percent of aspartic acid than those in histone-like dna binding protein ii (hu) of escherichia coli. the first ...	1988	3065334
scanning tunnelling microscopy of biomacromolecules.	when imaging biomacromolecules with a stm, coating of specimens with a conductive layer is a convenient preparation method which provides a good rate of success. utilizing evaporated platinum/carbon as a coating film we have investigated two biomacromolecules of very different appearance. the first of these is the hpi-layer, a natural two-dimensional protein crystal with a period of 18 nm, which is found on the surface of the bacterium deinococcus radiodurans. the second specimen is type iv coll ...	1988	3254999
deinococcus radiodurans uv endonuclease beta dna incisions do not generate photoreversible thymine residues.	the ability of uv endonuclease beta of deinococcus radiodurans to act as a pyrimidine dimer dna glycosylase was investigated. cell-free extracts of d. radiodurans exhibiting uv endonuclease beta activity failed to generate incisions in irradiated dna that liberated free-thymine residues upon photoreversal with 254-nm light. this is in marked contrast to the pyrimidine dimer uv glycosylase of micrococcus luteus that does liberate such residues. the result suggests that uv endonuclease beta incise ...	1988	3357488
lipid composition, lipid fluidity and radioresistance of deinococcus radiodurans and two mutant strains.	the lipid composition of d. radiodurans strain r1 and of two mutant strains has been studied in relation to membrane fluidity and sensitivity to x-ray radiation. no significant difference in the unsaturation degree of fatty acids was found between parental and mutant strains. an important decrease of carbohydrate-containing lipids was observed in the radiosensitive mutant strain. we also observed a higher fluidity in both mutant strains than in the parental one. modification of membrane lipid fl ...	1987	3129029
nucleotide sequence analysis of the gene encoding the deinococcus radiodurans surface protein, derived amino acid sequence, and complementary protein chemical studies.	the complete nucleotide sequence of the gene encoding the surface (hexagonally packed intermediate [hpi])-layer polypeptide of deinococcus radiodurans sark was determined and found to encode a polypeptide of 1,036 amino acids. amino acid sequence analysis of about 30% of the residues revealed that the mature polypeptide consists of at least 978 amino acids. the n terminus was blocked to edman degradation. the results of proteolytic modification of the hpi layer in situ and mr estimations of the ...	1987	3667529
molecular cloning, expression, and characterization of the gene for the surface (hpi)-layer protein of deinococcus radiodurans in escherichia coli.	the hpi protein of deinococcus radiodurans belongs to the class of surface layer proteins which form crystalline two-dimensional arrays on bacterial cell envelopes. we have cloned and expressed the gene for this protein of mr about 100,000 by using plasmid puc8 in escherichia coli. as judged by immunoreaction with monospecific antibodies, apparent mr, and limited proteolysis, a single clone contained the gene encoding the complete polypeptide on an 8.9-kilobase (kb) insert. the insert was reduce ...	1986	3528123
sensitivity of deinococcus radiodurans to near-ultraviolet radiation.	although dienococcus radiodurans is notoriously resistant to far-ultraviolet radiation (fuv; 254 nm), it is highly sensitive to near-ultraviolet radiation (nuv; 300-400 nm), thus demonstrating that the mechanisms of damage (and/or recovery) by the two types of irradiation are different. this observed difference between fuv and nuv effects in d. radiodurans agrees with previous studies with escherichia coli. near-ultraviolet radiation produces dna damage which is presumed to be single-strand brea ...	1986	3748048
three-dimensional structure of the regular surface layer (hpi layer) of deinococcus radiodurans.	the low-resolution structure of the regular surface layer of deinococcus radiodurans has been determined from negatively stained specimens by three-dimensional electron microscopy. the layer has p6 symmetry, a lattice constant of 18 nm and a thickness of 6.5 nm. three-dimensional reconstruction was performed by a hybrid real space/fourier space approach that incorporates partial compensation of lattice distortions: the model obtained is discussed in the light of independent information about the ...	1986	3701865
identification and initial characterisation of a pyrimidine dimer uv endonuclease (uv endonuclease beta) from deinococcus radiodurans; a dna-repair enzyme that requires manganese ions.	an endonuclease that incises lightly ultraviolet-irradiated supercoiled plasmid dna was identified in cell-free extracts of deinococcus radiodurans r1 wild-type. the endonuclease was absent from strains mutant in the uvsc, uvsd or uvse genes identifying it as 'uv endonuclease beta' responsible for the initial incision step of one excision-repair pathway for the removal of pyrimidine dimers from d. radiodurans dna in vivo. the enzyme was purified free from contaminating nuclease activities and wa ...	1985	3838572
structure of a novel phosphoglycolipid from deinococcus radiodurans.	the chemical structure of a major phosphoglycolipid from deinococcus radiodurans has been shown to be 2'-o-(1,2-diacyl-sn-glycero-3-phospho)-3'-o-(alpha-galactosyl)-n-d-gl yceroyl alkylamine. by infrared spectroscopy, the lipid was shown to contain both carbonyl ester and amide linkages. chemical analysis demonstrated a molar ratio of fatty acid, carbohydrate, and phosphorus of 2:1:1. the lipid was shown to contain an sn-3-phosphatidic acid backbone by digestion with phospholipase a2. phosphodie ...	1985	4044593
the cytotoxic and photodynamic inactivation of micro-organisms by rose bengal.	rose bengal was cytotoxic to the following bacteria at the concentrations given in parentheses (highest concentrations of dye in mol/l at which growth occurred on nutrient medium): brochothrix thermosphacta and deinococcus radiodurans (1 x 10(-6) or less); streptococcus, micrococcus, staphylococcus, bacillus, arthrobacter and kurthia spp. (1 x 10(-5)-1 x 10(-4], and pseudomonas spp. and enterobacteriaceae (5 x 10(-3)-1 x 10(-2) or greater). these organisms were killed rapidly when suspended in i ...	1985	3997691
cloning of the dna repair genes mtca, mtcb, uvsc, uvsd, uvse and the leub gene from deinococcus radiodurans.	a gene library from deinococcus radiodurans has been constructed in the cosmid pjbfh. a 51.5-kb hybrid cosmid, pue40, that transduced escherichia coli hb101 from leucine dependence to independence was selected, and a 6.9-kb fragment which carried the leub gene from d. radiodurans was subcloned into the ecori site of pat153. the dna repair genes mtca, mtcb, uvsc, uvsd and uvse, which code for two d. radiodurans uv endonucleases were identified by transforming appropriate repair-deficient mutants ...	1985	2989093
the plasmids of deinococcus spp. and the cloning and restriction mapping of the d. radiophilus plasmid pue1.	plasmids were found in strains representing all four species of the genus deinococcus viz. d. radiodurans, d. radiopugnans, d. radiophilus and d. proteolyticus but were not found in the most intensively-investigated strain of the genus, d. radiodurans r1. their sizes were calculated from electron micrographs. d. radiophilus yielded three size classes of plasmid while d. radiodurans sark, d. proteolyticus and d. radiopugnans each yielded two. attempts to cure d. radiophilus and d. radiodurans sar ...	1985	2986569
two new restriction endonucleases draii and draiii from deinococcus radiophilus.	in addition to recently characterized drai (1), two new type ii restriction endonucleases, draii and draiii, with novel site-specificities were isolated and purified from deinococcus radiophilus atcc 27603. draii and draiii recognize the hepta- and nonanucleotide sequences (sequence in text) the cleavage sites within both strands are indicated by arrows. the recognition sequences were established by mapping of the cleavage sites on pbr322 (draii) and fd109 rf dna (draiii). the sequence specifiti ...	1985	2987827
polar lipids from the radiation resistant bacterium deinococcus radiodurans: structural investigations on glucosaminyl and n-acetyl glucosaminyl lipids.	deinococcus radiodurans, although a gram-positive bacterium, has a complex cell wall with multiple layers and associates to this structural particularity, a quite unusual lipid composition for gram-positive bacteria. the conventional phospholipids (phosphatidyl ethanolamine, phosphatidyl choline, phosphatidyl glycerol...) are absent. among the nine polar lipids detected in the r1 anderson strain, three are glycolipids only one is a phospholipid, the other ones are glycophospholipids. one of the ...	1984	6331531
roles of the uvsc, uvsd, uvse, and mtca genes in the two pyrimidine dimer excision repair pathways of deinococcus radiodurans.	in deinococcus radiodurans, the genes uvsc, uvsd, uvse, and mtca are all involved in the single-strand incision of uv-irradiated dna, and mutations in at least two of them were required to produce an incisionless strain. one mutation must be in mtca and one in uvsc, uvsd, or uvse. strains carrying single mutations in any one of the genes can incise dna to the same extent as the wild-type strain. neither the presence of edta nor the absence of protein synthesis affected the incision step. strains ...	1983	6313608
mutation induction by cross-links in dna of deinococcus radiodurans.	reversion of adenine auxotrophic strains of d. radiodurans by cross-links in dna was studied. mutation was induced in a wild-type but not in a mitomycin c sensitive mutant after irradiation with near uv (approximately 365nm) in the presence of 4,5',8-trimethylpsoralen (tmp). to get information on implication of cross-links in the observed mutation, cells containing monoadducts in dna were exposed to near uv in the absence of tmp. the mutation frequency of the wild-type increased with increasing ...	1983	6847660
isolation and characterisation of drai, a type ii restriction endonuclease recognising a sequence containing only a:t basepairs, and inhibition of its activity by uv irradiation of substrate dna.	a type ii restriction endonuclease, drai, isolated from deinococcus radiophilus atcc 27603 recognises the palindromic hexanucleotide sequence (formula; see text) and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. the yield of enzyme is 100 to 1000 times that of the only other known type ii restriction endonuclease that recognises a sequence composed solely of a:t basepairs, the isoschizomer ahaiii (1). ultraviolet irradiation of the dna substrate at relatively low dose ...	1983	6310500
dna double-strand breakage and removal of cross-links in deinococcus radiodurans.	mitomycin c-sensitive mutants of deinococcus radiodurans were isolated which were either resistant to or only moderately sensitive to far uv (254 nm) or gamma rays. they were also sensitive to irradiation at 365 nm in the presence of 4,5',8-trimethylpsoralen. they were classified into seven complementary groups (mtca through mtcg) by transformation experiments. interstrand cross-links in the dna duplex induced by mitomycin c were removed in the cells of two mutants (mtcd and mtce) as in the wild ...	1983	6411683
isolation and properties of strains of micrococcus (deinococcus) radiodurans unable to excise ultraviolet light-induced pyrimidine dimers from dna: evidence for two excision pathways.	a mutant of deinococcus (formerly micrococcus) radiodurans (strain 302, mutant in mtca) sensitive to both the lethal effect of mitomycin c and the mutagenic effect of simple alkylating agents, but having wild-type resistance to uv light, was treated with the mutagen n-methyl-n'-nitro-n-nitrosoguanidine in an attempt to isolate strains deficient in the ability to excise uv-induced pyrimidine dimers. three strains were isolated that were uv-sensitive, but had wild-type resistance to the lethal eff ...	1983	6415229
cell division in deinococcus radiodurans and a method for displaying septa.	the study of sections, freeze-cleaved, and whole-cell preparations of deinococcus radiodurans supported the contention that septa close assymmetrically and originate from discrete opposing locations on the cell surface. tetrads and the larger associations (sheets) of cells in some strains were formed by alternate and synchronized divisions in two planes. the polarity initiating the second division in cells of the sark strain, in particular, was often expressed in slower growing cells before comp ...	1983	6661703
lack of ultraviolet mutagenesis in radiation-resistant bacteria.	ultraviolet (uv) radiation did not induce rifampicin-resistant mutants in populations of the taxonomically-related radiation-resistant bacteria deinococcus radiodurans, d. radiopugnans, d. radiophilus and d. proteolyticus, although such mutants arose spontaneously at a low frequency and at a high frequency after treatment of cultures with n-nitroso compounds. the radiation-resistant bacteria arthrobacter radiotolerans and p-30-a were also uv-immutable whereas the more radiation-sensitive pseudom ...	1982	7110166
isolation and characterization of the plasma membrane and the outer membrane of deinococcus radiodurans strain sark.	deinococcus radiodurans strain sark, although gram-positive, has a complex cell wall profile that includes an outer membrane-like structure. the outer cell envelope layers formed blebs throughout the growth cycle, which were shed as large vesicles (0.5-3.5 micron m in diameter) from approximately 5% of the cell population. instability was accentuated by treatment with 10% nacl, which released the outer membrane from all cells without disrupting the peptidoglycan layer, and provided an outer memb ...	1981	7296407