Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| phosphatidylcholine coatings deliver local antimicrobials and reduce infection in a murine model: a preliminary study. | phosphatidylcholine coatings have been shown to elute antibiotics for several days. a recently developed biofilm inhibitor, cis-2-decenoic acid (c2da), has been shown to exhibit synergistic activity with several common antibiotics. this study aims to evaluate the effectiveness of c2da and amikacin dual drug delivery from a phosphatidylcholine coating. | 2017 | 28050817 |
| pseudomonas aeruginosa groel stimulates production of ptx3 by activating the nf-κb pathway and simultaneously downregulating microrna-9. | as one of the first lines of host defense, monocytes play important roles in clearing infected microbes. the defensive response is triggered by recognition of diverse microbial moieties, including released factors, which modulate host immune responses to establish a harsh environment for clinically important bacterial pathogens. in this study, we found that the expression of ptx3, a soluble form of pattern recognition receptor, was induced by infection with live pseudomonas aeruginosa or treatme ... | 2017 | 28031262 |
| staphylococcus aureus survives in cystic fibrosis macrophages forming a reservoir for chronic pneumonia. | staphylococcus aureus (s. aureus) plays an important role in sepsis, pneumonia, wound infections and cystic fibrosis (cf), which is caused by mutations of the cystic fibrosis transmembrane conductance regulator (cftr). pulmonary s. aureus infections in cf often occur very early and prior to colonization with other pathogens, in particular pseudomonas aeruginosa here, we demonstrate that cf mice are highly susceptible to pulmonary infections with s. aureus and fail to clear the pathogen during in ... | 2017 | 28289144 |
| effects of silver nanoparticles on multiple drug-resistant strains of staphylococcus aureus and pseudomonas aeruginosa from mastitis-infected goats: an alternative approach for antimicrobial therapy. | recently, silver nanoparticles (agnps) have been widely used in various applications as antimicrobial agents, anticancer, diagnostics, biomarkers, cell labels, and drug delivery systems for the treatment of various diseases. microorganisms generally acquire resistance to antibiotics through the course of antibacterial therapy. multi-drug resistance (mdr) has become a growing problem in the treatment of infectious diseases, and the widespread use of broad-spectrum antibiotics has resulted in the ... | 2017 | 28272303 |
| 5-hydroxyethyl-3-tetradecanoyltetramic acid represents a novel treatment for intravascular catheter infections due to staphylococcus aureus. | biofilm infections of intravascular catheters caused by staphylococcus aureus may be treated with catheter lock solutions (clss). here we investigated the antibacterial activity, cytotoxicity and cls potential of 5-hydroxyethyl-3-tetradecanoyltetramic acid (5he-c14-tma) compared with the related compounds 3-tetradecanoyltetronic (c14-toa) and 3-tetradecanoylthiotetronic (c14-tta), which are variants of quorum sensing signalling molecules produced by pseudomonas aeruginosa . | 2017 | 27999062 |
| pathogenesis of microbial keratitis. | microbial keratitis is a sight-threatening ocular infection caused by bacteria, fungi, and protist pathogens. epithelial defects and injuries are key predisposing factors making the eye susceptible to corneal pathogens. among bacterial pathogens, the most common agents responsible for keratitis include staphylococcus aureus, pseudomonas aeruginosa, streptococcus pneumonia and serratia species. fungal agents of corneal infections include both filamentous as well as yeast, including fusarium, aspe ... | 2017 | 27998732 |
| thiol-ol chemistry for grafting of natural polymers to form highly stable and efficacious antibacterial coatings. | bacterial contamination of surfaces and the associated infection risk is a significant threat to human health. some natural antibacterial polymers with low toxicity are promising coating materials for alleviating pathogenic colonization on surfaces. however, widespread application of these polymers as antibacterial coatings is constrained by coating techniques which are not easily scalable due to stringent reaction conditions. herein, thiol-ol reaction involving oxidative conjugation between thi ... | 2017 | 27991755 |
| antibacterial and non-cytotoxic ultra-thin polyethylenimine film. | in recent years, a common strategy, to obtain more uniform and controlled synthesis of polyelectrolytes multilayers (pems), relies on a previous polyethylenimine (pei) coating of the substrate surface. pei is a synthetic cationic polymer which provides a positive charge distribution on the materials to be covered with pems. despite being an important step, this pre-layer deposition is frequently overlooked and no comprehensive characterizations or deep discussions are reported in literature. in ... | 2017 | 27987765 |
| a comparative study of different strategies for removal of endotoxins from bacteriophage preparations. | bacterial endotoxins have high immunogenicity. phage biology studies as well as therapeutic phage applications necessitate highly purified phage particles. in this study, we compared combinations of seven different endotoxin removal strategies and validated their endotoxin removal efficacy for five different phages (i.e. four pseudomonas aeruginosa phages and one staphylococcus aureus phage). these purification strategies included endotrap hd column purification and/or cscl density centrifugatio ... | 2017 | 27913133 |
| sonodynamic inactivation of gram-positive and gram-negative bacteria using a rose bengal-antimicrobial peptide conjugate. | combating antimicrobial resistance is one of the most serious public health challenges facing society today. the development of new antibiotics or alternative techniques that can help combat antimicrobial resistance is being prioritised by many governments and stakeholders across the globe. antimicrobial photodynamic therapy is one such technique that has received considerable attention but is limited by the inability of light to penetrate through human tissue, reducing its effectiveness when us ... | 2017 | 27908581 |
| controlled extended octenidine release from a bacterial nanocellulose/poloxamer hybrid system. | although bacterial nanocellulose (bnc) has been widely investigated in the last 10years as drug delivery system, up to now no long-term controlled release of drugs could be realized. therefore, the aim of the present work was the development of a bnc-based drug delivery system that provides prolonged retention time for the antiseptic octenidine up to one week with improved mechanical and antimicrobial properties as well as a high biocompatibility. bnc was modified by incorporation of differently ... | 2017 | 27889415 |
| antimicrobial metabolites from the plant endophytic fungus penicillium sp. | five rare dichloro aromatic polyketides (1-5) were obtained from an endophytic fungus penicillium sp., along with five known metabolites (6-10). their structures were elucidated by extensive spectroscopic analysis, mosher methods, as well as [rh2(ococf3)4]-induced electronic circular dichroism (ecd) experiments. compounds 2-4 and 6 structurally involved acyclic 1.3-diols, the uneasy configuration determinations of which were well carried out by double-derivation nmr methods. compounds 1-10 were ... | 2017 | 27876600 |
| is there still a role for irrigation and debridement with liner exchange in acute periprosthetic total knee infection? | periprosthetic joint infection (pji) is an important cause of failure in total knee arthroplasty. irrigation and debridement including liner exchange (i&d/l) success rates have varied for acute pji. the purpose of this study is to present results of a specific protocol for i&d/l with retention of total knee arthroplasty components. | 2017 | 27876465 |
| glycoside hydrolases degrade polymicrobial bacterial biofilms in wounds. | the persistent nature of chronic wounds leaves them highly susceptible to invasion by a variety of pathogens that have the ability to construct an extracellular polymeric substance (eps). this eps makes the bacterial population, or biofilm, up to 1,000-fold more antibiotic tolerant than planktonic cells and makes wound healing extremely difficult. thus, compounds which have the ability to degrade biofilms, but not host tissue components, are highly sought after for clinical applications. in this ... | 2017 | 27872074 |
| cadexomer iodine provides superior efficacy against bacterial wound biofilms in vitro and in vivo. | examination of clinical samples indicates bacterial biofilms are present in the majority of chronic wounds, and substantial evidence suggests biofilms contribute significantly to delayed healing. bacteria in biofilms are highly tolerant of antimicrobials, and little data exist to guide the choice of anti-biofilm wound therapy. cadexomer iodine (ci) was recently reported to have superior efficacy compared to diverse wound dressings against pseudomonas aeruginosa biofilms in an ex vivo model. in t ... | 2017 | 27859922 |
| combination of grape extract-silver nanoparticles and liposomes: a totally green approach. | in the present work, silver nanoparticles were prepared using a totally green procedure combining silver nitrate and an extract of grape pomace as a green source. additionally, nanoparticles were stabilized using phospholipid and water and/or a mixture of water and propylene glycol (pg). to the best of our knowledge, grape-silver nanoparticle stabilized liposomes or pg-liposomes were formulated, for the first time, combining the residual products of wine-made industry, silver nitrate and phospho ... | 2017 | 27825918 |
| development and evaluation of silver sulfadiazine loaded microsponge based gel for partial thickness (second degree) burn wounds. | silver sulfadiazine has been frequently used as an antibacterial agent for topical treatment of partial thickness burn wounds. in this study, we present the preparation of silver sulfadiazine microsponges by w/o/w emulsion solvent evaporation method. formulation variables were optimized by using 3(2) factorial design. the optimized microsponges were characterized by ftir, dsc, pxrd, particle size analysis, sem analysis and mercury intrusion porosimetry studies. viscosity, texture analysis and ex ... | 2017 | 27697504 |
| h2o2 and/or tio2 photocatalysis under uv irradiation for the removal of antibiotic resistant bacteria and their antibiotic resistance genes. | inactivating antibiotic resistant bacteria (arb) and removing antibiotic resistance genes (args) are very important to prevent their spread into the environment. previous efforts have been taken to eliminate arb and args from aqueous solution and sludges, however, few satisfying results have been obtained. this study investigated whether photocatalysis by tio2 was able to reduce the two args, meca and ampc, within the host arb, methicillin-resistant staphylococcus aureus (mrsa) and pseudomonas a ... | 2017 | 27776873 |
| simple and versatile method for creation of non-leaching antimicrobial surfaces based on cross-linked alkylated polyethyleneimine derivatives. | novel quaternized polyethyleneimine and cross-linked polyethyleneimine derivatives have been synthesized using both traditional and microwave-assisted techniques to create antimicrobial coatings, with octyl, dodecyl, or hexadecyl bromides as alkylating agent and various bifunctional electrophiles as cross-linkers. quaternization has been performed using methyl iodide or dimethyl sulfate; it has been shown that methyl iodide has no advantages over dimethyl sulfate. antimicrobial activity of the p ... | 2017 | 27770956 |
| cystic fibrosis - comparison between patients in paediatric and adult age. | cystic fibrosis (cf) is the most common autosomal recessive disease in caucasians. although most cases are diagnosed in childhood, diagnosis in adults is apparently increasing. | 2017 | 27743767 |
| risk factors for drug-resistant pathogens in immunocompetent patients with pneumonia: evaluation of pes pathogens. | the new acronym, pes pathogens (pseudomonas aeruginosa, enterobacteriaceae extended-spectrum beta-lactamase-positive, and methicillin-resistant staphylococcus aureus), was recently proposed to identify drug-resistant pathogens associated with community-acquired pneumonia. | 2017 | 27729192 |
| first record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa. | maggot debridement therapy (mdt) consists on the intentional and controlled application of sterilized larvae of the order diptera on necrotic skin lesions with the purpose of cleaning necrotic tissue and removing pathogenic bacteria. during mdt, a marked antimicrobial activity has been reported in literature specially associated with antibacterial substances from lucilia sericata (meigen); however, regarding cochliomyia macellaria (fabricius), little is known. this study aimed to evaluate in vit ... | 2017 | 27696133 |
| respiratory pathogen colonization of dental plaque, the lower airways, and endotracheal tube biofilms during mechanical ventilation. | in mechanically ventilated patients, the endotracheal tube is an essential interface between the patient and ventilator, but inadvertently, it also facilitates the development of ventilator-associated pneumonia (vap) by subverting pulmonary host defenses. a number of investigations suggest that bacteria colonizing the oral cavity may be important in the etiology of vap. the present study evaluated microbial changes that occurred in dental plaque and lower airways of 107 critically ill mechanical ... | 2017 | 27621110 |
| essential oil components of orange peels and antimicrobial activity. | in this study, the orange peel of 12 cultivars of citrus sinensis from central-eastern sicily was employed to obtain essential oils and extracts. the ones were extracted through steam distillation, the others through extraction in hexane. chemical constituents were evaluated in terms of qualitative and quantitative analyses by gas chromatography/mass spectrometry. fifty-four components were identified in the steam essential oils and 44 in the extracts. in all the cultivars, the main component is ... | 2017 | 27535797 |
| increasing incidence of multidrug resistance among cystic fibrosis respiratory bacterial isolates. | pseudomonas aeruginosa and staphylococcus aureus are common pathogens in cystic fibrosis (cf) patients with increasing multidrug resistance (mdr). this study characterized antimicrobial susceptibility trends among organisms isolated from the respiratory tract of cf patients. microbiological culture and sensitivity results for all cf patients were collected from january 2010 through december 2014. minimum inhibitory concentrations were obtained using phoenix(®) and etest(®) methods. clinical and ... | 2017 | 27326758 |
| bacterial chondritis complications following ear piercing. | body piercing has become widespread and is associated with increased complications. post-piercing chondritis may lead to severe residual deformity. we aimed to report case patients presenting with post-piercing chondritis in our department and to describe clinical features and treatment. | 2017 | 27596713 |
| ciprofloxacin-imprinted hydrogels for drug sustained release in aqueous media. | in this study several ciprofloxacin (cfx) imprinted and non-imprinted hydrogels were prepared and evaluated as ocular drug delivery systems in aqueous media. 2-hydroxyethyl methacrylate (hema) was used as a solvent and backbone monomer, ethylene glycol dimethacrylate (egdma) as a cross-linker, methacrylic acid (maa) as a functional monomer and cfx as the template molecule. cfx-imprinted hydrogels (mips) were prepared applying different cfx:maa molar ratios (1:16, 1:20 and 1:32) in feed compositi ... | 2017 | 27585481 |
| trends in antibiotic resistance in bacterial keratitis isolates from south india. | to report trends in antibiotic resistance in cases of bacterial keratitis from a large eye hospital in south india. | 2017 | 27130916 |
| green synthesis of silver nanoparticles using rheum palmatum root extract and their antibacterial activity against staphylococcus aureus and pseudomonas aeruginosa. | in the present study, silver nanoparticles (agnps) were synthesized by using aqueous root extracts of rheum palmatum and characterized by various spectroscopic methods. the nanoparticles were found to be in hexagonal and spherical shapes. the average particle size was found to be 121 ± 2 nm with zeta potential values of -21.6 mv by dynamic light scattering (dls) method. gas chromatography-mass spectrometry (gc-ms) analysis of r. palmatum revealed 35 compounds. the synthesized agnps showed signif ... | 2017 | 27023851 |
| surveillance of bacteria pseudomonas aeruginosa and mrsa associated with chronic suppurative otitis media. | suppurative otitis media is a critical disease causing perforation of the tympanic membrane associated with changes of the mucoperiosteum of the middle ear cleft. | 2017 | 27177975 |
| organoselenium polymer inhibits biofilm formation in polypropylene contact lens case material. | contact lens-acquired bacterial infections are a serious problem. of the reported cases, inadequate cleaning of the lens case was the most common cause of lens contamination. organoselenium has been shown to inhibit bacterial attachment to different polymer materials. this study evaluates the ability of an organoselenium monomer, incorporated into the polymer of a polypropylene contact lens case coupon, to block the formation of biofilms in a lens case. | 2017 | 26974534 |
| [incidence and risk factors associated with nosocomial infection in pediatric heart surgery]. | nosocomial infections are responsible for a high rate of morbidity and mortality in pediatric patients undergoing heart surgery. our objective was to determine the incidence and associated risk factors to nosocomial infections in this group of patients. | 2017 | 26960046 |
| targeting bacterial biofilms via surface engineering of gold nanoparticles. | bacterial biofilms are associated with persistent infections that are resistant to conventional antibiotics and substantially complicate patient care. surface engineered nanoparticles represent a novel, unconventional approach for disruption of biofilms and targeting of bacterial pathogens. herein, we describe the role of surface charge of gold nanoparticles (aunps) on biofilm disruption and bactericidal activity towards staphylococcus aureus and pseudomonas aeruginosa which are important ventil ... | 2017 | 26877871 |
| how antibiotics should be prescribed to hospitalized elderly patients with community-acquired pneumonia. | elderly patients hospitalized with community-acquired pneumonia (cap) should be administered antimicrobials in the emergency department prior to transfer to the ward or intensive care unit (icu). for ward patients, a β-lactam with a macrolide or a respiratory fluoroquinolone alone should be given to cover typical and atypical pathogens. for icu patients, a β-lactam with either a macrolide or a fluoroquinolone should be given. other regimens are indicated if methicillin-resistant staphylococcus a ... | 2017 | 27928779 |
| in vitro comparison of epidural bacteria filters permeability and screening scanning electron microscopy. | epidural catheter bacteria filters are barriers in the patient-controlled analgesia/anaesthesia for preventing contamination at the epidural insertion site. the efficiency of these filters varies according to pore sizes and materials. | 2017 | 26614147 |
| in vitro parallel evaluation of antibacterial activity and cytotoxicity of commercially available silver-containing wound dressings. | this study evaluated the in vitro antibacterial activity and cytotoxicity of various commercially available silver-containing dressings (ag dressing). | 2017 | 26605826 |
| oxidation resistance 1 (oxr1) participates in silkworm defense against bacterial infection through the jnk pathway. | bacterial infection causes enhanced reactive oxygen species (ros) levels in insects. oxidation resistance 1 (oxr1) plays an antioxidant role in eukaryotic organisms, including insects. in this report, we demonstrated that pseudomonas aeruginosa and staphylococcus aureus infection and hydrogen peroxide (h2 o2 ) injection induced the expression of specific transcriptional isoforms of oxr1 in larval silkworms. we further showed that a jun kinase (jnk) pathway inhibitor, sp600125, down-regulated exp ... | 2017 | 26507465 |
| nanoemulsion therapy for burn wounds is effective as a topical antimicrobial against gram-negative and gram-positive bacteria. | the aim of this study is to investigate the antimicrobial efficacy of two different nanoemulsion (ne) formulations against gram-positive and gram-negative bacteria in an in vivo rodent scald burn model. male sprague-dawley rats were anesthetized and received a partial-thickness scald burn. eight hours after burn injury, the wound was inoculated with 1 × 10(8) colony-forming units of pseudomonas aeruginosa or staphylococcus aureus. treatment groups consisted of two different ne formulations (nb-2 ... | 2017 | 26182074 |
| time course study of delayed wound healing in a biofilm-challenged diabetic mouse model. | bacterial biofilm has been shown to play a role in delaying wound healing of chronic wounds, a major medical problem that results in significant health care burden. a reproducible animal model could be very valuable for studying the mechanism and management of chronic wounds. our previous work showed that pseudomonas aeruginosa (pao1) biofilm challenge on wounds in diabetic (db/db) mice significantly delayed wound healing. in this wound time course study, we further characterize the bacterial bu ... | 2017 | 22564229 |
| infectious keratitis in south australia: emerging resistance to cephazolinq. | to analyze the microbiologic spectrum and patterns of resistance of infectious keratitis in patients treated at a tertiary hospital in south australia. | 2017 | 28221427 |
| treatment of acute bacterial conjunctivitis with topical lomefloxacin 0.3% compared to topical ofloxacin 0.3. | the main purpose of this prospective study was to compare the efficacy, local tolerance, and safety of topical lomefloxacin 0.3% and topical ofloxacin 0.3% in the treatment of acute bacterial conjunctivitis. patiens and methods. forty patients with acute bacterial conjunctivitis were included in a randomized, prospective, parallel-group study. twenty patients were assigned to the lomefloxacin group (okacin, ciba vision ophthalmics) and 20 patients to ofloxacin (oflox, allergan). lomefloxacin 0.3 ... | 2017 | 28252678 |
| active antibacterial and antifouling surface coating via a facile one-step enzymatic cross-linking. | prevention of microbial contamination of surfaces is one of the biggest challenges for biomedical applications. establishing a stable, easily produced, highly antibacterial surface coating offers an efficient solution but remains a technical difficulty. here, we report on a new approach to create an in situ hydrogel film-coating on glass surfaces made by enzymatic cross-linking under physiological conditions. the cross-linking is catalyzed by horseradish peroxidase (hrp)/glucose oxidase (god)-co ... | 2017 | 28005352 |
| long-chain alkyl cyanides: unprecedented volatile compounds released by pseudomonas and micromonospora bacteria. | the analysis of volatiles from bacterial cultures revealed long-chain aliphatic nitriles, a new class of natural products. such nitriles are produced by both gram-positive micromonospora echinospora and gram-negative pseudomonas veronii bacteria, although the structures differ. a variable sequence of chain elongation and dehydration in the fatty acid biosynthesis leads to either unbranched saturated or unsaturated nitriles with an ω-7 double bond, such as (z)-11-octadecenenitrile, or methyl-bran ... | 2017 | 28276609 |
| evaluation of lactic acid as an antibacterial agent. | fifty strains each of staphylococcus aureus, beta haemolytic streptococci, proteus species, esch coli and pseudomonas aeruginosa were subjected to 2%, 1 % and 0. 1 % lactic acid in peptorie water. minimum inhibitory concentration of lactic acid for all the strains of each of these organisms was 0.1% or 1%. depending upon its concentration, lactic acid added to peptone water brings down the ph to 2.5-4 which by itself has some inhibitory effect on the microorganisms. lactic acid however, retains ... | 2017 | 28223586 |
| production of valuable compounds by molds and yeasts. | we are pleased to dedicate this paper to dr julian e davies. julian is a giant among microbial biochemists. he began his professional career as an organic chemistry phd student at nottingham university, moved on to a postdoctoral fellowship at columbia university, then became a lecturer at the university of manchester, followed by a fellowship in microbial biochemistry at harvard medical school. in 1965, he studied genetics at the pasteur institute, and 2 years later joined the university of wis ... | 2017 | 27731337 |
| butyrate influences intracellular levels of adenine and adenine derivatives in the fungus penicillium restrictum. | butyrate, a small fatty acid, has an important role in the colon of ruminants and mammalians including the inhibition of inflammation and the regulation of cell proliferation. there is also growing evidence that butyrate is influencing the histone structure in mammalian cells by inhibition of histone deacetylation. butyrate shows furthermore an antimicrobial activity against fungi, yeast and bacteria, which is linked to its toxicity at a high concentration. in fungi there are indications that bu ... | 2017 | 28219521 |
| microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity. | ultrahigh-throughput screening (uhts) techniques can identify unique functionality from millions of variants. to mimic the natural selection mechanisms that occur by compartmentalization in vivo, we developed a technique based on single-cell encapsulation in droplets of a monodisperse microfluidic double water-in-oil-in-water emulsion (mde). biocompatible mde enables in-droplet cultivation of different living species. the combination of droplet-generating machinery with facs followed by next-gen ... | 2017 | 28202731 |
| bacterial reduction and shift with npwt after surgical debridements: a retrospective cohort study. | surgical debridement, negative-pressure wound therapy (npwt) and antibiotics are used for the treatment of open wounds. however, it remains unclear whether this treatment regimen is successful in the reduction and shift of the bacterial load. | 2017 | 27988849 |
| chi3l1 regulation of inflammation and the effects on osteogenesis in a staphylococcus aureus-induced murine model of osteomyelitis. | osteomyelitis is an inflammation of the bone and bone marrow that occurs as a consequence of infections mainly attributed to staphylococcus aureus. in a previous study, we found that expression of the chitinase 3-like 1 (chi3l1) gene affected mineralization of mc3t3-e1 cells infected with s. aureus and there was increased expression of chi3l1 in the blood of osteomyelitis patients. in the present study, to further investigate the role of chi3l1 in osteomyelitis, we developed an s. aureus-induced ... | 2017 | 28391634 |
| study on the utility of a statewide counselling programme for improving mortality outcomes of patients with staphylococcus aureus bacteraemia in thuringia (support): a study protocol of a cluster-randomised crossover trial. | staphylococcus aureus bacteraemia (sab) is a frequent infection with high mortality rates. it requires specific diagnostic and therapeutic management such as prolonged intravenous administration of antibiotics and aggressive search for and control of infectious sources. underestimation of disease severity frequently results in delayed or inappropriate management of patients with sab leading to increased mortality rates. according to observational studies, patient counselling by infectious diseas ... | 2017 | 28391236 |
| diverse mobilization strategies facilitate transfer of non-conjugative mobile genetic elements. | conjugation is a dominant mechanism of horizontal gene transfer and substantially contributes to the plasticity and evolvability of prokaryotic genomes. the impact of conjugation on genetic flux extends well beyond self-transmissible conjugative elements, because non-conjugative 'mobilizable elements' utilize other elements' conjugative apparatus for transfer. bacterial genome comparisons highlight plasmids as vehicles for dissemination of pathogenesis and antimicrobial-resistance determinants, ... | 2017 | 28391142 |
| screening for nasal carriage of staphylococcus aureus among patients scheduled to undergo orthopedic surgery: incidence of surgical site infection by nasal carriage. | staphylococcus aureus (s. aureus), including mrsa, is considered to be the leading cause of surgical site infection (ssi) after orthopedic surgery. we screened for nasal carriers of s. aureus among patients who were scheduled to undergo orthopedic surgery at our hospital to reveal the effect of nasal s. aureus carriage on ssi. our study design clearly has the intent of finding s. aureus nasal carriage and eradicating mrsa when found, and this strategy is to verify whether it's effective for prev ... | 2017 | 28390756 |
| early local delivery of vancomycin suppresses ectopic bone formation in a rat model of trauma-induced heterotopic ossification. | heterotopic ossification (ho) is a debilitating sequela of high-energy injuries. it frequently requires surgical excision once symptomatic and there is no practical prophylaxis for combat-injured patients. in this study, we examined the effect of local vancomycin powder on ho formation in a small animal model of blast-related, post-traumatic ho. male sprague-dawley rats were subjected to a polytraumatic extremity injury and amputation with or without methicillin-resistant staphylococcus aureus i ... | 2017 | 28390182 |
| antimicrobial agents for preventing peritonitis in peritoneal dialysis patients. | peritoneal dialysis (pd) is an important therapy for patients with end-stage kidney disease and is used in more than 200,000 such patients globally. however, its value is often limited by the development of infections such as peritonitis and exit-site and tunnel infections. multiple strategies have been developed to reduce the risk of peritonitis including antibiotics, topical disinfectants to the exit site and antifungal agents. however, the effectiveness of these strategies has been variable a ... | 2017 | 28390069 |
| influence of molecular characteristics in the prognosis of methicillin-resistant staphylococcus aureus prosthetic joint infections: beyond the species and the antibiogram. | we have explored the relationship of phenotypic (antibiogram, β-haemolysis, agr functionality, biofilm formation) and genotypic characteristics on the prognosis of 18 cases of methicillin-resistant s. aureus prosthetic joint infection (2005-2015). all isolates belonged to cc5, and had agr type ii. this pilot study suggests that phage-borne genes belonging to the immune evasion cluster (chp, sak and scn) were more frequent among episodes with treatment failure (80.0 vs. 37.5%). | 2017 | 28389926 |
| carcinogenic activities and sperm abnormalities of methicillin resistance staphylococcus aureus and inhibition of their virulence potentials by ayamycin. | this investigation aimed to study the in vivo harmful effects of the subcutaneous injection of different methicillin resistance staphylococcus aureus extracts (mrsa2, mrsa4, mrsa10, mrsa69, mrsa70, mrsa76, and mrsa78). such strains represented the highest minimum inhibition concentration toward methicillin with various multidrug-resistant patterns. the obtained results revealed that rats injected with the mrsa4 extract died immediately after the last dose indicating the high cytotoxicity of mrsa ... | 2017 | 28389766 |
| airborne pathogens inside automobiles for domestic use: assessing in-car air decontamination devices using staphylococcus aureus as the challenge. | family cars represent ∼74% of the yearly global output of motorized vehicles. with the life-expectancy of ∼8 decades in many countries, one spends >100 minutes daily inside the confined and often shared space of the car with exposure to a mix of potentially harmful microbes. can commercial in-car air microbial decontamination devices mitigate the risk? three such devices (designated as device #s1-3) with hepa filters were tested in the modified passenger cabin (3.25 m3) of a four-door sedan hous ... | 2017 | 28389537 |
| canine scent detection as a tool to distinguish meticillin-resistant staphylococcus aureus. | 2017 | 28389090 | |
| biomimetic bacterial identification platform based on thermal wave transport analysis (twta) through surface imprinted polymers. | this article introduces a novel bacterial identification assay based on thermal wave analysis through surface-imprinted polymers (sips). aluminum chips are coated with sips, serving as synthetic cell receptors that have been combined previously with the heat-transfer method (htm) for the selective detection of cancer cells, macrophages and, more recently, bacteria. in this work, the concept of bacterial identification is extended towards the detection of nine different bacterial species. in addi ... | 2017 | 28388095 |
| inhibition of fracture healing in the presence of contamination by staphylococcus aureus: effects of growth state and immune response. | extremity injuries comprise a significant portion of trauma, affecting quality of life, financial burden, and return to duty. bacterial contamination is commonly associated with failure to heal, despite antibiotic treatment, suggesting that additional therapies must be developed to combat these complications. treatment failure is likely due to the presence of resistant microbial communities known as biofilms. biofilm bacteria are able to elicit a direct inhibition of healing through a multitude ... | 2017 | 28387956 |
| does intrawound vancomycin application during spine surgery create vancomycin-resistant organism? | surgical site infection (ssi) following spine surgery is a morbid and expensive complication. the use of intrawound vancomycin is emerging as a solution to reduce ssi. the development of vancomycin-resistant pathogens is an understandable concern. | 2017 | 28387851 |
| hydrogels with modulated ionic load for mammalian cell harvesting with reduced bacterial adhesion. | in this manuscript, we describe the fabrication of hydrogel supports for mammalian cell handling that can simultaneously prevent materials from microbial contamination and therefore allow storage in aqueous media. for that purpose, hydrogels based on the antifouling polymer polyvinylpyrrolidone (pvp) were functionalized with different ionic groups (anionic, cationic or two types of zwitterions). in order to prevent bacterial adhesion in a long term, we took advantage of the synergistic effect of ... | 2017 | 28387521 |
| targeting microbial biofilms using ficin, a nonspecific plant protease. | biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. we demonstrate here the destruction of staphylococcus aureus and staphylococcus epidermidis bio ... | 2017 | 28387349 |
| real-world experience with oritavancin therapy in invasive gram-positive infections. | oritavancin is a novel lipoglycopeptide approved for acute bacterial skin and skin structure infections. the pharmacokinetic profile and convenient one-time dosing make oritavancin an enticing option for other serious gram-positive infections requiring prolonged treatment courses. unfortunately, data for using oritavancin in these populations are limited. | 2017 | 28386776 |
| inhibition of biofilm formation on iodine-supported titanium implants. | we have developed iodine-supported titanium implants that suppress microbial activities and conducted in vivo and in vitro studies to determine their antimicrobial properties. | 2017 | 28386730 |
| study on synthesis and bioactivity of biotinylated emodin. | a novel compound biotinylated emodin was synthesized by a two-step acyl chloride method which connects the biotin to emodin with esterification reaction. the product was characterized with ultraviolet-visible spectrophotometry, fourier transform infrared and high-performance liquid chromatography tandem mass spectrometry techniques. its antibacterial activity against staphylococcus aureus cmcc 26003 was investigated, and the emodin- and biotinylated emodin-caused antibacterial mechanism was prop ... | 2017 | 28386632 |
| fabrication and characterization of electrospun poly(e-caprolactone) fibrous membrane with antibacterial functionality. | this research study is mainly targeted on fabrication and characterization of antibacterial poly(e-caprolactone) (pcl) based fibrous membrane containing silver chloride particles. micro/nano fibres were produced by electrospinning and characterized with tga, dsc, sem and mechanical analysis. it was found that addition of silver particles slightly reduced onset of thermal degradation and increased crystallization temperature of neat pcl. silver-loaded samples exhibited higher tensile stress and l ... | 2017 | 28386444 |
| identification of ellagic acid rhamnoside as a bioactive component of a complex botanical extract with anti-biofilm activity. | staphylococcus aureus is a leading cause of hospital-acquired infections. it is listed among the top "serious threats" to human health in the usa, due in large part to rising rates of resistance. many s. aureus infections are recalcitrant to antibiotic therapy due to their ability to form a biofilm, which acts not only as a physical barrier to antibiotics and the immune system, but results in differences in metabolism that further restricts antibiotic efficacy. development of a modular strategy ... | 2017 | 28386254 |
| evaluation of hiaureus(tm) coagulase confirmation kit in identification of staphylococcus aureus. | staphylococcus aureus is a facultative anaerobic gram positive coccal bacterium whose incidence ranges to different infections. it is a cause of various uncomplicated skin infections, abscesses, septicaemia/bacteraemia, gastroenteritis, endocarditis, toxic shock syndrome and food intoxications. various methods with varied time, sensitivities, specificities and costs are available, but may not be used as a reliable test for the identification and differentiation of s. aureus. therefore, there is ... | 2017 | 28384859 |
| methicillin-resistant staphylococcus aureus as a uropathogen in an irish setting. | urinary tract infections are one of the most common infectious diseases diagnosed in the community and in the hospital setting. their treatment is complicated by drug-resistant pathogens and the colonization by microbes of indwelling urinary catheters. this study assessed the occurrence and antimicrobial susceptibility of methicillin-resistant staphylococcus aureus (mrsa) uropathogens isolated for 5 consecutive years at university hospital waterford between 2010 and 2014. we created 4 clinically ... | 2017 | 28383394 |
| current clinical trials on the use of ceftaroline in the pediatric population. | the rate of antibiotic resistance in children continues to rise requiring the use of new antibiotics. ceftaroline fosamil, a newer-generation cephalosporin, was recently approved for the treatment of acute bacterial skin and skin structure infections and community-acquired bacterial pneumonia in children aged >2 months. ceftaroline provides coverage against staphylococcal and streptococcal infections, including methicillin-resistant staphylococcus aureus and penicillin-resistant streptococcus pn ... | 2017 | 28382572 |
| development of novel antibiotic lysocin e identified by silkworm infection model. | in this symposium, we reported the identification and mechanistic analysis of a novel antibiotic named lysocin e. lysocin e was identified by screening for therapeutic effectiveness in a silkworm staphylococcus aureus infection model. the advantages of the silkworm infection model for screening and purification of antibiotics from the culture supernatant of soil bacteria are: 1) low cost; 2) no ethical issues; 3) convenient for evaluation of the therapeutic effectiveness of antibiotics; and 4) p ... | 2017 | 28381711 |
| a novel multiplex pcr assay for the detection of chlorhexidine/quaternary ammonium, mupirocin and methicillin resistance genes with simultaneous discrimination of staphylococcus aureus from coagulase-negative staphylococci. | methicillin-resistant staphylococcus aureus (mrsa) is a clinically significant pathogen resistant to a wide variety of antibiotics and responsible for a large number of nosocomial infections worldwide. the agency for healthcare research and quality and the centers for disease control and prevention recently recommended to adopt universal mupirocin/chlorhexidine decolonization of all admitted intensive care unit patients, rather than mrsa screening with targeted treatments, which raises the serio ... | 2017 | 28381601 |
| localization of staphylococcus aureus in tissue from the nasal vestibule in healthy carriers. | colonization of the body is an important step in staphylococcus aureus infection. s. aureus colonizes skin and mucous membranes in humans and several animal species. one important ecological niche of s. aureus is the anterior nares. more than 60% of the s. aureus in the nose are found in vestibulum nasi. our aim was to describe the localization of s. aureus in nasal tissue from healthy carriers. | 2017 | 28381253 |
| high vancomycin mics predict the development of infective endocarditis in patients with catheter-related bacteraemia due to methicillin-resistant staphylococcus aureus. | it has been suggested that there is an increased risk of treatment failure in episodes of mrsa bloodstream infection (bsi) caused by strains with high vancomycin mics. however, it is unknown if this phenomenon may also act as a risk factor for the development of infective endocarditis (ie). | 2017 | 28379553 |
| the rna targetome of staphylococcus aureus non-coding rna rsaa: impact on cell surface properties and defense mechanisms. | the virulon of staphyloccocus aureus is controlled by intricate connections between transcriptional and post-transcriptional regulators including proteins and small non-coding rnas (srnas). many of the srnas regulate gene expression through base-pairings with mrnas. however, characterization of the direct srna targets in gram-positive bacteria remained a difficult challenge. here, we have applied and adapted the ms2-affinity purification approach coupled to rna sequencing (maps) to determine the ... | 2017 | 28379505 |
| definitive treatment for methicillin-sensitive staphylococcus aureus bacteremia: data versus a definitive answer? | 2017 | 28379366 | |
| an automated, pharmacist-driven initiative improves quality of care for staphylococcus aureus bacteremia. | infectious diseases (id) consultation and antimicrobial stewardship intervention have been shown to improve the management of staphylococcus aureus bacteremia (sab). as the workload of antimicrobial stewardship programs (asps) continues to increase, asps must find a way to maximize the efficiency of the program while optimizing patient outcomes. the objective of this study was to evaluate the impact of incorporating health informatics into the management of sab via a pharmacist-driven initiative ... | 2017 | 28379326 |
| comparative effectiveness of cefazolin versus nafcillin or oxacillin for treatment of methicillin-susceptible staphylococcus aureus infections complicated by bacteremia: a nationwide cohort study. | to treat patients with methicillin-susceptible s. aureus (mssa) infections, beta-lactams are recommended for definitive therapy; however the comparative effectiveness of individual beta-lactams is unknown. this study compared definitive therapy with cefazolin versus nafcillin or oxacillin among patients with mssa infections complicated by bacteremia. | 2017 | 28379314 |
| characteristics and clinical assessment of antibiotic delivery by chitosan sponge in the high-risk diabetic foot: a case series. | the local delivery of antimicrobials is attractive for a number of reasons. chitosan, a biodegradable polysaccharide sponge material, has been proposed as medium to deliver antibiotics directly to wounds. in this report we evaluate the safety and practicality of antimicrobial delivery via chitosan sponge. | 2017 | 28379101 |
| albumin reduces the antibacterial efficacy of wound antiseptics against staphylococcus aureus. | the influence of proteins on the efficacy of antiseptic solutions has been rarely investigated even though exudate can contain high levels of protien. the aim of this study was to analyse the antibacterial efficacy of commonly used solutions in the presence of albumin protein. | 2017 | 28379100 |
| an insight into the antibiofilm properties of costa rican stingless bee honeys. | there is an increasing search for antibiofilm agents that either have specific activity against biofilms or may act in synergy with antimicrobials. our objective is to examine the the antibiofilm properties of stingless bee honeys. | 2017 | 28379095 |
| translational pharmacometric evaluation of typical antibiotic broad-spectrum combination therapies against staphylococcus aureus exploiting in vitro information. | broad-spectrum antibiotic combination therapy is frequently applied due to increasing resistance development of infective pathogens. the objective of the present study was to evaluate two common empiric broad-spectrum combination therapies consisting of either linezolid or vancomycin combined with meropenem against staphylococcus aureus as the most frequent causative pathogen of severe infections. a semi-mechanistic pk-pd model mimicking a simplified bacterial life-cycle of s. aureus was develop ... | 2017 | 28378945 |
| histones from avian erythrocytes exhibit antibiofilm activity against methicillin-sensitive and methicillin-resistant staphylococcus aureus. | staphylococcus aureus, a human pathogen associated with many illnesses and post-surgical infections, can resist treatment due to the emergence of antibiotic-resistant strains and through biofilm formation. the current treatments for chronic biofilm infections are antibiotics and/or surgical removal of the contaminated medical device. due to higher morbidity and mortality rates associated with overuse/misuse of antibiotics, alternate treatments are essential. this study reports the antibiofilm ac ... | 2017 | 28378802 |
| diagnosis and management of skin and soft-tissue infections (ssti). a literature review and consensus statement: an update. | skin and soft-tissue infections (sstis) are among the most common bacterial infections, posing considerable diagnostic and therapeutic challenges. fourteen members of the italian society of infectious diseases, after a careful review of the most recent literature using medline database and their own clinical experience, updated a previous paper published in 2011 by preparing a draught manuscript of the statements. the manuscript was successively reviewed by all members and ultimately re-formulat ... | 2017 | 28378613 |
| inhalable andrographolide-β-cyclodextrin inclusion complexes for treatment of staphylococcus aureus pneumonia by regulating immune responses. | bacterial pneumonia is a serious disease with high mortality if no appropriate and immediate therapy is available. andrographolide (ag) is an anti-inflammatory agent extracted from a traditional chinese herb andrographis paniculata. oral ag tablets and pills are clinically applied for treatment of upper respiratory tract infections. however, the low solubility and bioavailability of ag lead to high doses and long-term therapy. here we developed an andrographolide-β-cyclodextrin inclusion complex ... | 2017 | 28378587 |
| efficacy of vaccination and nisin z treatments to eliminate intramammary staphylococcus aureus infection in lactating cows. | this study evaluated the effect of a staphylococcus aureus bacterin and nisin on bovine subclinical mastitis. a total of 75 holstein subclinically mastitic cows were randomly allocated to three groups with 25 cows per group. in group i, an intramammary infusion of nisin z at a dose of 2.50×10(6) iu was carried out once daily for three days, and an autogenous s. aureus bacterin was inoculated into the supramammary lymph node one week before and one week after nisin treatment. in group ii, nisin w ... | 2017 | 28378574 |
| staphylococcus aureus enterotoxin-sensitization is associated with allergic poly-sensitization and allergic multimorbidity in adolescents. | staphylococcus aureus (s. aureus) carriage and sensitization to s. aureus enterotoxins (ses) has been associated with allergic diseases. from the tromsø study fit futures 2, we have previously shown an association between s. aureus carriage and severe allergic disease and allergic multimorbidity. however, the role of s. aureus carriage and se-sensitization on allergic multimorbidity and allergic sensitization is unclear. | 2017 | 28378344 |
| il-17a plays an important role in protection induced by vaccination with fibronectin-binding domain of fibronectin-binding protein a against staphylococcus aureus infection. | fibronectin-binding protein a (fnbpa) of staphylococcus aureus is a microbial surface component recognizing adhesive matrix molecules and has been known as one of the most important virulence factors involved in the initiation step of s. aureus infection. therefore, it has been considered as a potential vaccine candidate. previous studies have reported that vaccination with fnbpa protects animals against s. aureus infection. in this study, we demonstrated that vaccination with fibronectin-bindin ... | 2017 | 28378247 |
| ventilator-associated pneumonia by methicillin-susceptible staphylococcus aureus: do minimum inhibitory concentrations to vancomycin and daptomycin matter? | the use of vancomycin minimum inhibitory concentration (mic) as an outcome predictor in patients with methicillin-susceptible staphylococcus aureus (mssa) bacteremia has become an important topic for debate in the last few years. given these previous results, we decided to investigate whether mics to vancomycin or daptomycin had any effect on the evolution of patients with ventilator-associated pneumonia (vap) due to mssa. an observational, retrospective, multicenter study was conducted among pa ... | 2017 | 28378244 |
| prolonged suppressive antibiotic therapy for prosthetic joint infection in the elderly: a national multicentre cohort study. | during prosthetic joint infection (pji), optimal surgical management with exchange of the device is sometimes impossible, especially in the elderly population. thus, prolonged suppressive antibiotic therapy (psat) is the only option to prevent acute sepsis, but little is known about this strategy. we aimed to describe the characteristics, outcome and tolerance of psat in elderly patients with pji. we performed a national cross-sectional cohort study of patients >75 years old and treated with psa ... | 2017 | 28378243 |
| [antibiotic stewardship and staphylococcus aureus bacteremia]. | rates of antibiotic resistance are increasing worldwide and impact on the treatment of patients with bacterial infections. a broad and uncritical application in inpatient and outpatient settings as well as in agriculture has been recognized as the main driving force. antibiotic stewardship (abs) programs aim at countering this worrisome development using various direct interventions such as infectious disease counseling. blood stream infections caused by staphylococcus (s.) aureus are severe inf ... | 2017 | 28378151 |
| defining motility in the staphylococci. | the ability of bacteria to move is critical for their survival in diverse environments and multiple ways have evolved to achieve this. two forms of motility have recently been described for staphylococcus aureus, an organism previously considered to be non-motile. one form is called spreading, which is a type of sliding motility and the second form involves comet formation, which has many observable characteristics associated with gliding motility. darting motility has also been observed in stap ... | 2017 | 28378043 |
| host response to staphylococcus epidermidis colonization and infections. | the majority of research in the staphylococcus field has been dedicated to the understanding of staphylococcus aureus infections. in contrast, there is limited information on infections by coagulase-negative staphylococci (cons) and how the host responds to them. s. epidermidis, a member of the coagulase-negative staphylococci, is an important commensal organism of the human skin and mucous membranes; and there is emerging evidence of its benefit for human health in fighting off harmful microorg ... | 2017 | 28377905 |
| antimicrobial resistance and molecular characterization of staphylococcus aureus causing childhood pneumonia in shanghai. | staphylococcus aureus or methicillin-resistant staphylococcus aureus (mrsa) is a major pathogen causing pneumonia among children. to estimate the prevalence and molecular properties of s. aureus in children pneumonia in shanghai, china, 107 hospitalized children with s. aureus pneumonia from two children's hospitals from january 2014 through june 2015 were studied. s. aureus isolates from the respiratory specimens were characterized by antimicrobial susceptibility, agr typing, toxin genes, multi ... | 2017 | 28377752 |
| vlp-based vaccine induces immune control of staphylococcus aureus virulence regulation. | staphylococcus aureus is the leading cause of skin and soft tissue infections (sstis) and mounting antibiotic resistance requires innovative treatment strategies. s. aureus uses secreted cyclic autoinducing peptides (aips) and the accessory gene regulator (agr) operon to coordinate expression of virulence factors required for invasive infection. of the four agr alleles (agr types i-iv and corresponding aips1-4), agr type i isolates are most frequently associated with invasive infection. cyclizat ... | 2017 | 28377579 |
| novel boronic acid derivatives of bis(indolyl) methane as anti-mrsa agents. | towards the search for a new generation of antibiotics to control methicillin-resistant staphylococcus aureus (mrsa), the design and synthesis of various bis indolyl methane (bim) derivatives based on their different electron donor and acceptor properties of the substituents have been made, in which boronic acid derivatives of bim are found to be active against mrsa. the observed evidence with the lead compound reveals their strong anti-mrsa activity, which paves the way of design and further de ... | 2017 | 28377058 |
| in vitro transfer of methicillin resistance determinants meca from methicillin resistant staphylococcus aureus (mrsa) to methicillin susceptible staphylococcus aureus (mssa). | staphylococcus aureus more than any other human pathogen is a better model for the study of the adaptive evolution of bacterial resistance to antibiotics, as it has demonstrated a remarkable ability in its response to new antibiotics. this study was designed to investigate the in vitro transfer of meca gene from methicillin resistant s. aureus to methicillin susceptible s. aureus. | 2017 | 28376716 |
| impact of type i and iii interferons on respiratory superinfections due to multidrug-resistant pathogens. | the increased morbidity and mortality associated with bacterial pneumonias that are acquired following influenza infection are well appreciated by clinicians. one of the major components of the immune response to influenza is the induction of the types i and iii interferon cascades, which encompasses the activation of over 300 genes. the immunological consequences of ifn activation, while important for viral clearance, modify the host proinflammatory responses through effects on the inflammasome ... | 2017 | 28375519 |
| life after usa300: the rise and fall of a superbug. | the community-associated methicillin-resistant staphylococcus aureus (ca-mrsa) epidemic in the united states is largely attributable to the meteoric rise of a single clone, referred to as usa300. this strain not only spread across the united states in just a few years to become the predominant cause of staphylococcal disease, but it also appears to have increased the overall number of skin and soft-tissue infections (sstis), increasing the overall disease burden. while usa300 still constitutes a ... | 2017 | 28375517 |