Publications

oropharynx microbiota among alcoholics and non-alcoholics.	the oropharynx microbiota plays an important role in the origin of infections, especially among alcoholics whose airway defenses are impaired.	1998	9876451
trichomonas tenax empyema in an immunocompromised patient with advanced cancer.	a 53-year-old male acromegalic patient with advanced rectal adenocarcinoma developed pleuritis in the course of cobalt irradiation, steroid treatment and chemotherapy. examination of drained pleural fluid demonstrated numerous motile organisms, which were identified as trichomonas tenax by giemsa staining. peptostreptococcus micros was also detected in the cultures of pleural fluid and blood. treatment with metronidazole successfully eliminated the protozoa and cured the pyothorax.	1998	9879561
a commensal symbiosis between prevotella bivia and peptostreptococcus anaerobius involves amino acids: potential significance to the pathogenesis of bacterial vaginosis.	in both batch and continuous culture, peptostreptococcus anaerobius was able to grow in vaginal defined medium with prevotella bivia, but not in pure culture. growth of p. anaerobius was increased by 238% (p < 0.001) in peptone-supplemented vaginal defined medium conditioned by prior growth of p. bivia. analysis of p. bivia culture supernatants showed a net accumulation of amino acids and subsequent growth of p. anaerobius in the conditioned supernatants resulted generally in amino acid utilizat ...	1998	9879923
pathogenicity of peptostreptococcus micros morphotypes and prevotella species in pure and mixed culture.	recently, an atypical rough colony morphotype of peptostreptococcus micros, a species which is found in ulcerating infections, including periodontitis, was isolated. the virulence of morphotypes alone and in combination with prevotella intermedia and p. nigrescens was investigated both in vivo and in vitro. all strains tested induced abscesses containing fluid pus in a mouse skin model, and lesions caused by monocultures of the rough morphotype strains of p. micros were statistically significant ...	1998	9879956
[clinical evaluation of lung abscess diagnosed by transtracheal aspiration].	we have diagnosed lung abscess according to findings of infiltration with cavity formation on chest x-ray and/or ct-scan and pathogens isolated from transtracheal aspirates. we evaluated the clinical features of 20 patients with lung abscess (18 males and 2 females, mean age; 54.3 years). diabetus mellitus and periodontal diseases were prominent underlying diseases in patients with lung abscess. cough was complained in 13 patients, chest or back pain in 9, purulent sputum in 8 and hemosputum in ...	1998	9884505
microbiology of perianal cellulitis in children: comparison of skin swabs and needle aspiration.	to establish the aerobic and anaerobic microbiology of perianal cellulitis in children, comparing skin swab and needle aspirate methodology.	1998	9888333
spondylitis caused by peptostreptococcus.	peptostreptococcus is an anaerobic gram-positive coccus that is encountered in dental and sinus infections, pelvic infections in women, osteomyelitis, arthritis, skin and soft-tissues infections, conjunctivitis and bacteraemia [1,2]. we report here, what is to our knowledge, the first cases of spondylitis with discitis caused by peptostreptococcus.	1998	9890688
comparative anti-anaerobic activity of men 10700, a penem antibiotic.	the in vitro activity of men 10700, a new penem, has been compared with that of metronidazole, clindamycin, ciprofloxacin, co-amoxiclav, imipenem and three third generation cephalosporins against 120 strains of anaerobes. the organisms tested comprised clostridium perfringens, clostridium difficile, bacteroides fragilis and speciated members of the genera fusobacterium, veillonella and peptostreptococcus. men 10700 showed activity similar to that of imipenem, and was more potent than metronidazo ...	1998	9916909
aerobic and anaerobic microbiology of dacryocystitis.	to investigate the aerobic and anaerobic microbiology of dacryocystitis.	1998	9559743
the sequences of small proteins are not extensively optimized for rapid folding by natural selection.	the thermodynamic stabilities of small protein domains are clearly subject to natural selection, but it is less clear whether the rapid folding rates typically observed for such proteins are consequences of direct evolutionary optimization or reflect intrinsic physical properties of the polypeptide chain. this issue can be investigated by comparing the folding rates of laboratory-generated protein sequences to those of naturally occurring sequences provided that the method by which the sequences ...	1998	9560214
aerobic and anaerobic microbiology of retroperitoneal abscesses.	the aerobic and anaerobic microbiology of various types of retroperitoneal abscesses was studied by review of the clinical and laboratory data for 161 patients treated between 1974 and 1990 for such abscesses. these included 109 anterior, 8 posterior, 21 retrofascial, and 23 pelvic retroperitoneal abscesses. a total of 472 organisms (2.9 isolates/specimen)--204 aerobic and facultative (1.3/specimen), and 268 anaerobic (1.7/specimen)--were recovered. aerobes only were recovered from 34 abscesses ...	1998	9564479
supernatant cytotoxicity and proteolytic activity of selected oral bacteria against human gingival fibroblasts in vitro.	the purpose of this study was to determine if endodontic bacterial act in vitro on human gingival fibroblast functions via extracellular products. the bacteria used were prevotella nigrescens, capnocytophaga ochracea, peptostreptoccocus micros and actinobacillus actinomycetemcomitans. supernatants were collected from bacterial cultures at the beginning of the stationary phase when their density was similar. toxins that inhibited fibroblast proliferation were found in all culture supernatants of ...	1998	9569986
ammonia-hyperproducing bacteria from new zealand ruminants.	pasture-grazed dairy cows, deer, and sheep were tested for the presence of ammonia-hyperproducing (hap) bacteria in roll tubes containing a medium in which tryptone and casamino acids were the sole nitrogen and energy sources. colonies able to grow on this medium represented 5.2, 1.3, and 11.6% of the total bacterial counts of dairy cows, deer, and sheep, respectively. a total of 14 morphologically distinct colonies were purified and studied further. restriction fragment length polymorphisms of ...	1998	9572953
effect of red blood cells on the growth of porphyromonas endodontalis and microbial community development.	establishment of a microbial community in the root canal system depends on numerous factors, of which nutrient availability may be one of the most important. we hypothesized that the presence of red blood cells or hemoglobin in this environment could cause shifts in microbial composition of communities, resulting in organisms such as porphyromonas endodontalis becoming more dominant. an in vitro model system using mixed, batch cultures was performed with the bacteria p. endodontalis, fusobacteri ...	1998	9573801
subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation.	this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ...	1998	9573815
prevalence of peptostreptococcus micros morphotypes in patients with adult periodontitis.	the prevalence of the smooth and rough colonial morphotypes of peptostreptococcus micros was examined with culture technique in 123 patients with adult periodontitis (age 24-68 years). of all subgingival samples, 91% contained the smooth morphotype of p. micros. the smooth morphotype constituted a mean percentage of the total anaerobic viable biota of 6.0%, with a range of 0.02-35.7%. of these samples, 49% contained colonies of the rough morphotype as well, with a mean percentage of the total an ...	1998	9573826
infected stingray injury.		1998	9576395
tracheal botryomycosis in a patient with tracheopathia osteochondroplastica.	tracheal botryomycosis has never been reported in the literature. a 68 year old man presented with progressive dyspnoea and an exophytic mass below the vocal cords was found by bronchoscopy. pathological study of the mass showed tracheal botryomycosis probably associated with peptostreptococcus spp infection. the patient was given intravenous penicillin for six weeks. tracheal obstruction necessitated tracheal resection and the surgical specimen revealed typical findings of tracheopathia osteoch ...	1998	9577526
aerobic and anaerobic bacteriology of otorrhea associated with tympanostomy tubes in children.	the microbiology of in 55 ear aspirates obtained from 34 children with chronic otorrhea was studied. aspiration of the middle ear exudate was done immediately following removal of tympanostomy tube (tt). the middle ear aspirates and swab specimens of the external auditory canals were cultured for aerobic and anaerobic bacteria. sixty-five isolates were recovered only from the middle ears, 73 only from the external ear canals, and 73 were present at both sites. analysis of the 138 middle ear isol ...	1998	9583788
[subacute infectious endocarditis in prosthetic valve by peptostreptococcus micros].		1998	9586375
bacterial interference in the nasopharynx following antimicrobial therapy of acute otitis media.	the effect on the nasopharyngeal bacterial flora of therapy for 10 days with co-amoxiclav or cefprozil was studied in 50 children with acute otitis media. before therapy, potential pathogens (streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis) were isolated in 14 (56%) of those treated with co-amoxiclav and 15 (60%) of those treated with cefprozil. following therapy, the reduction in the number of these pathogens was the same in the two groups. however, differences betwee ...	1998	9598781
aminopeptidase activities in peptostreptococcus spp. are statistically correlated to gelatin hydrolysis.	one hundred peptostreptococcus isolates from five species were assessed for their ability to hydrolyze gelatin. most peptostreptococcus magnus (95.8%) and peptostreptococcus micros isolates (79.0%) hydrolyzed gelatin in contrast to peptostreptococcus asaccharolyticus (8.0%), peptostreptococcus anaerobius (10.0%), and peptostreptococcus prevotii isolates (16.7%). gelatin hydrolysis in peptostreptococcus magnus and peptostreptococcus micros isolates correlated (r = 0.80; p = 0.0019) with more amin ...	1998	9606913
partial purification of an iron-dependent l-serine dehydratase from clostridium sticklandii.	an oxygen-sensitive and highly unstable l-serine dehydratase was partially purified from the gram-positive anaerobe clostridium sticklandii. the final active preparation contained five proteins of 27, 30, 44.5, 46, and 58 kda as judged by sds-page. the n-terminal sequence of the 30 kda subunit showed some similarity to the alpha-subunits of the iron-containing l-serine dehydratases from clostridium propionicum and peptostreptococcus asaccharolyticus. oxygen-inactivated l-serine dehydratase from ...	1998	9637015
osteomyelitis of the fibula due to septic embolism: a rare complication of a graft enteric erosion. case report.	we report the case of a 70-year-old female who experienced a graft enteric erosion (gee) three years after a right aorto-femoral bypass. she was treated by right axillo-femoral bypass, removal of the aortic prosthesis and direct aortic suture. the duodenum was stitched up and separated from the aorta by omentoplasty. bacteriological culture of the removed graft identified escherichia coli and peptostreptococcus. antibiotic therapy consisting of amoxicillin and gentamycin was given for seven days ...	1998	9639000
aerobic and anaerobic microbiology of infections after trauma in children.	to review the recovery of aerobic and anaerobic bacteria from infections after trauma in children over a 20 year period.	1998	9639177
development of an anaerobic bacterial leakage model.	the majority of bacteria associated with infections of endodontic origin are strict anaerobes. the purpose of this study was to develop an endodontic microleakage model using strict anaerobic bacteria in a two-chamber system. nine species of anaerobic bacteria were tested for viability and detection by either turbidity or color change of the broth. a survey of ph chromogenic substrates revealed that bromcresol purple (ph 5.2 = yellow, ph 6.8 = purple) could be used as a chromogenic indicator to ...	1998	9641124
endodontic pathogens stimulate monocyte chemoattractant protein-1 and interleukin-8 in mononuclear cells.	microbial infection of the dental pulp leads to the recruitment of leukocytes and the formation of lesions of endodontic origin. the chemokines interleukin-8 (il-8) and monocyte chemoattractant protein-1 (mcp-1) are relatively specific chemoattractants for neutrophils and monocytes, respectively. in the present studies, peripheral blood mononuclear cells were stimulated by streptococcus mutants, porphyromonas endodontalis, and peptostreptococcus anaerobius, which are associated with lesions of e ...	1998	9641137
[antimicrobial activity of macrolides against clinical isolates].	antimicrobial activity of 6 macrolides was determined using a micro-broth dilution method, against 535 clinical isolates of 22 species, which were isolated in 1996 from 325 facilities in japan. results were as follows. 1. in general, antimicrobial activities of 14-membered macrolides were higher than those of 16-membered macrolides. the antimicrobial activities of 14-membered macrolides were in the order of clarithromycin (cam), erythromycin (em), roxithromycin (rxm). among 16-membered macrolide ...	1998	9644599
aerobic and anaerobic microbiology of chronic venous ulcers.	the role of bacteria in the pathogenesis of chronic venous leg ulcers (cvlu) is unclear. the objective of the study was to establish the aerobic and anaerobic bacteriology of cvlu.	1998	9646126
microbiology of cervical lymphadenitis in adults.	the microbiology of needle aspirates from 40 inflamed cervical lymph glands was studied for aerobic and anaerobic bacteria, fungi and mycobacteria. forty-two bacterial, 11 mycobacterial and six fungal isolates were isolated. aerobic bacteria only were recovered in 11 (27.5%), anaerobes alone in five (12.5%) and mixed aerobic and anaerobic bacteria in seven (17.5%). mycobacterium sp. were recovered in 11 (27.5%) and fungi in six (15%). the recovery of anaerobes was associated with dental infectio ...	1998	9655225
comparative in vitro activity of bay 12-8039 and five other antimicrobial agents against anaerobic bacteria.	the in vitro activity of bay 12-8039 against 360 anaerobic clinical isolates was determined by the agar dilution method and compared to that of five other antimicrobial agents. bay 12-8039 and imipenem were the most active agents tested. the following mic90 values were determined for bay 12-8039: peptostreptococcus spp. (50 isolates), 1 mg/l; propionibacterium acnes (30 isolates). 0.25 mg/l; clostridium perfringens (30 isolates), 0.5 mg/l; clostridium difficile (50 isolates), 2 mg/l; bacteroides ...	1998	9665302
semiquantitative bacteriology of closed odontogenic abscesses.	pus samples from twenty-three dentoalveolar abscesses were collected by needle aspiration and examined by direct inoculation technique using 6 different aerobic and anaerobic agar plates. for aerobic culture, sheep blood agar and chocolate agar (kyokuto pharmaceutical co., tokyo, japan) were used. for anaerobic culture, four different media, 1) brucella hk agar with hemolyzed rabbit blood and defibrillated sheep blood, 2) paramomycin-vancomycin brucella hk agar with hemolyzed rabbit blood, 3) ph ...	1998	9667143
management of an infected hematometrocolpos in a patient with congenital adrenal hyperplasia and vaginal stenosis.		1998	9679917
in vitro and in vivo antibacterial activities of am-1155 in the fields of obstetrics and gynecology.	am-1155 is a new 8-methoxyquinolonecarboxylic acid with a broad spectrum of antibacterial activity. it inhibited more than 90% of clinical isolates of streptococcus agalactiae, escherichia coli, peptostreptococcus magnus, bacteroides fragilis and prevotella bivia at the concentration of 3.13 mg/l. the antibacterial activity of am-1155 was almost equal to that of sparfloxacin. the in vivo efficacy of am-1155 was evaluated using a polymicrobial pyometra (e. coli and b. fragilis) model of rats. the ...	1998	9681200
experimental root canal infections in conventional and germ-free mice.	a small animal model was evaluated to study the interrelationships between microorganisms after their implantation in root canals (inferior central incisors) using germ-free (gf) and conventional (cv) mice. the selected microorganisms were: porphyromonas endodontalis (atcc 35406), eubacterium lentum (atcc 25559), peptostreptococcus anaerobius (atcc 27337), fusobacterium nucleatum (atcc 10953), escherichia coli (atcc 25922), and enterococcus faecalis (atcc 4083). only p. anaerobius, e. coli, and ...	1998	9693583
female genital tract bacterial coisolates with candida albicans in patients without clinical vaginitis.	in vitro, candida albicans has demonstrated the ability to inhibit replication of selected bacteria. little information exists on the impact of c. albicans on the vaginal bacterial flora in vivo. the purpose of this study is to identify the coexisting bacterial flora when c. albicans is isolated from vaginal cultures submitted to a hospital-based testing facility for reasons other than vulvovaginitis.	1998	9702585
ovarian abscess caused by peptostreptococcus magnus following transvaginal ultrasound-guided aspiration of ovarian endometrioma and fixation with pure ethanol.	transvaginal ultrasound-guided aspiration of ovarian endometrioma has been applied and emphasized as a safe and simple procedure.	1998	9702588
[a case report of epidural abscess due to anaerobic bacteria, producing a mass of gas].	a 54-year-old female was admitted to our hospital on may 8, 1996, for evaluation of fever and headache. her cerebrospinal fluid showed elevation of the cell count and total protein. the thoracic and lumbar mris revealed abnormal gas occupying the epidural space at the sacral level, and extended as high as 9th thoracic level. the gas spread inside the iliopsoas and paraspinal muscles. anaerobic bacteria such as bacteroides fragilis and peptostreptococcus productus were cultured from the spinal fl ...	1998	9711118
superficial suppurative thrombophlebitis in children, caused by anaerobic bacteria.	the purpose of this study was to present the microbiology and clinical features of six children with superficial suppurative thrombophlebitis (sst) caused by anaerobic bacteria.	1998	9722004
subgingival microbial profile of papillon-lefèvre patients assessed by dna-probes.	the prevalence of 18 selected bacterial species was assessed by means of "checkerboard" dna-dna hybridisation in a group of 12 saudi-arabian adolescents with papillon-lefèvre syndrome. a total of 36 tooth sites were investigated. the patients exhibited severe periodontal disease with deep pockets. all 12 patients harboured the putative bacterial pathogens p. intermedia, f. nucleatum, p. micros and s. intermedius while t. denticola, b. forsythus, p. nigrescens, e. corrodens, s. noxia and c. rectu ...	1998	9722266
oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis.	the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ...	1998	9722273
preterm birth: associations with genital and possibly oral microflora.	opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ...	1998	9722706
in vitro activities of e1101, a novel oral cephalosporin, against bacteria causing infections in obstetric and gynecological patients.	e1101 is a new oral cephalosporin with a broad spectrum of antibacterial activity. it inhibited more than 90% of clinical isolates of streptococcus agalactiae, escherichia coli and peptostreptococcus magnus at the concentration of 3.13 mg/l. e1101 was the most active agent against s. agalactiae and e. coli. since none of the compounds was sufficiently active against the bacteroides fragilis and prevotella bivia isolates, they are not appropriate in the treatment of patients with infections cause ...	1998	9732148
in-vitro antibiotic susceptibility and molecular analysis of anaerobic bacteria isolated in cape town, south africa.	one hundred and twenty-three anaerobic isolates from cape town, south africa, were tested in vitro against cefoxitin, chloramphenicol, metronidazole, co-amoxiclav, benzylpenicillin and clindamycin. forty-five gram-positive organisms were tested against rp59500 (a streptogramin). resistance in the gram-positive isolates was as follows: of the clostridium perfringens isolates, 4% were resistant to benzylpenicillin and 4% to clindamycin; of the peptostreptococcus anaerobius isolates, 10% were resis ...	1998	9738844
increased antibody production against gut-colonizing escherichia coli in the presence of the anaerobic bacterium peptostreptococcus.	germ-free rats were colonized with e. coli alone, or with e. coli plus lactobacillus acidophilus and a strain of the obligate anaerobic gram-positive species, peptostreptococcus. the presence of peptostreptococcus reduced translocation of e. coli, but increased the serum antibody response to e. coli antigen. whereas the immunoglobulin g (igg) anti-e. coli antibodies largely represented cross-reactive antibodies, those of the immunoglobulin m (igm) isotype represented true anti-e. coli antibodies ...	1998	9743213
[a bacteriology analysis from infected root canals of human deciduous teeth]	objective: to provide a basis for therapy and prevention, the flora of infected root canals of human deciduous teeth was analyzed.methods: by immediate-enzyme method, the bacteria compositions of 22 cases with infected root canals of deciduous teeth, including 8 cases with acute periapical inflammation and 14 with chronic ones, were examined. results: among 240 strains bactera, 200 strains were obligate anaerobes, belonging to the genera peptostreptococcus, bacteriodes, veillonella, eubacterrum, ...	1998	15071650
the microbial spectrum of brain abscess with special reference to anaerobic bacteria.	the bacteriological and clinical findings of 18 cases of brain abscess are presented. identification and antimicrobial susceptibility of anaerobic strains was performed both by conventional methods and the newer rapid ana ii panel and e-test methods, respectively. characterization of the anaerobic isolates was done by restriction fragment length polymorphism (rflp). anaerobic or aerobic organisms alone were recovered in three (16.6%), and mixed aerobic and anaerobic in another three (16.6%) pati ...	1998	11013664
a prospective, randomized, clinical study to compare the clinical safety, effectiveness, and cost of oral ofloxacin/clindamycin vs intravenous clindamycin/gentamicin for the treatment of postpartum endomyometritis.	objective: the primary objective of this prospective, randomized, clinical study was to compare the safety, clinical and microbiologic efficacy, and cost of oral ofloxacin in combination with clindamycin vs intravenous (iv) clindamycin/gentamicin in the early empiric treatment for hospitalized patients with mild to moderate postpartum endomyometritis. the secondary objective is to reduce total hospital and patient treatment cost. postpartum endomyometritis is a major cause of infectious morbidit ...	1998	10838268
[endogenous intragastrical microflora in gastric cancer]	the aim of this prospective study was to identify the type of microbial flora and additionally to correlate the total number of microorganisms with factors such as age, tumoral area, and the ph gastric in unaffected gastric mucosal biopsies obtained during gastrojejunostomy (n=5) or gastrectomy (n=1) in patients with gastric adenocarcinoma (n=15) or lymphoma (n=1) at the belen hospital, trujillo, peru, between january 1, 1996 and april 30,1998. the mean age of the patients was 54.4 + 18.3 years ...	1998	12209218
[bacteriological study of chronic maxillary sinusitis in adults and observation of susceptibility to antibiotics].	the unilateral mucosal samples were taken from 42 patients and were cultured for bacteria. meanwhile, the antimicrobial susceptibility was determined for 11 antibiotics and the beta-lactamase-producing bacteria(beta lpb) were examined. in 85.71% of all samples, bacterial growth was present. anaerobic bacteria were present in 21.43% of cases. predominant aerobic bacteria were alpha-hemolytic streptococci and coagulase-negative staphylococci. predominant anaerobic bacteria were peptostreptococcus ...	1998	11263213
anaerobic meningitis due to peptostreptococcus species: case report and review.	we describe a patient with postsurgical anaerobic meningitis due to peptostreptococcus magnus. in cases of meningitis associated with peptostreptococcus species reported in the literature, the most common predisposing factors are meningorectal fistulae and head-and-neck surgery. most patients respond well to appropriate antimicrobial therapy. surgical intervention may be required in some instances.	1997	9431395
gram-positive resistance: challenge for the development of new antibiotics.	the incidence of infections caused by multidrug-resistant gram-positive organisms is increasing despite advances in antibacterial therapy over the last 20 years. as the pathogens causing these infections are frequently resistant to most currently available antibacterials, they are extremely difficult to treat. problematic pathogens include strains of streptococcus pneumoniae resistant to beta-lactams and macrolides, viridans group streptococci resistant to beta-lactams and aminoglycosides, enter ...	1997	9511055
[actinobacteriosis of paranasal sinuses].	on the base the review of literature and own case the authors presents diagnostic difficulties and therapy of paranasal sinuses actinobacteriosis. actinobacteriosis of sinuses is very rare case and diagnostic analysis based on mycologic and histopathologic investigations. in our case sinuses actinobacteriosis orbital abscess complication has been noted. antibiotic therapy and surgical treatment have been used in this case.	1997	9518322
fungi in therapy-resistant apical periodontitis.	the occurrence of yeasts in 967 microbiological endodontic samples taken from root canals in persistent endodontic infections was studied. the sampling was done by general practitioners in various parts of finland from root canal infections which did not respond favourably to standard conservative therapy. the samples were cultivated aerobically on a non-selective enriched horse blood agar medium, on tsbv agar medium in 5% co2 and anaerobically on horse blood agar medium. micro-organisms were fo ...	1997	10332243
post-caesarean septicaemia in kandang kerbau hospital, singapore, 1993-1995.	we reviewed all documented cases of septicaemia following caesarean deliveries in kandang kerbau hospital between 1st january 1993 to 31st december 1995. there were 22 cases of septicaemia among 8201 caesarean births, and hence the incidence is 2.7:1000. there were 45,412 deliveries, and the overall caesarean section rate was 18.1%. among the 22 documented cases of septicaemia which came under this study, the most common clinical conditions found were endomyometritis (7 cases), urinary tract inf ...	1997	10968108
peptostreptococcus vaginalis or peptostreptococcus prevotii? identification and clinical importance of a new species of peptostreptococcus.	peptostreptococcus vaginalis is a recently described species of gram-positive anaerobic coccus. we report one case in which p. vaginalis was isolated in pure culture from an abscess on the upper arm, and summarise nine further cases where it was isolated in mixed culture from other superficial sites, particularly infected leg ulcers. we suggest that clinical strains of p. vaginalis have probably been described in the past as peptostreptococcus prevotii, a species which has frequently been report ...	1997	16887558
anaerobes isolated from clinical material over three years.	data on anaerobic bacteria isolated from clinical specimens at the bacteriology department within the 3-year period (1992-1994) were analysed. anaerobic cultivation was carried out in all aspirates and swabs were transferred in transport media in syringes or blood cultures. established growth occurred in all samples cultivated in thioglycollate broth after 4 days of incubation. cultivation methods included enrichment media, gaspak jar, and api (biomerieux) for final identification. a sulfite-red ...	1997	16887566
periodontal microflora of hiv infected patients with periodontitis.	the aim of this study was to determine the microbial profile of periodontal lesions in hiv seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. the subgingival microflora of 20 cdc ii, 20 cdc iii, 20 cdc iv/v and 20 systemically healthy patients with rapidly progressing periodontitis was examined. four sites with greatest probing depth in each patient were selected for microbiological sampling. the samples were cultured aerobicall ...	1997	16887570
bilophila wadsworthiain brain abscess: case report.	a case of a patient with a 20-year history of chronic otitis media complicated by cholesteatoma and brain abscess is described. a ct scan with contrast material showed three abscess cavities in the right cerebellar hemisphere. a culture from a specimen of the cholesteatoma yielded a significant amount of growth of bilophila wadsworthia, bacteroides fragilis and prevotella oris and a moderate growth of alpha-streptococci and staphylococcus simulans. from the pus of the brain abscess we also isola ...	1997	16887572
subgingival microflora in smokers with early onset periodontitis.	cigarette smoking is a potent risk factor which has recently been associated with periodontal disease progression. the objective of this study was to detect the microbial profile of early onset periodontitis in smokers and compare it to that of non-smokers. the study population consisted of 50 systemically healthy individuals aged 25 to 38 years, exhibiting early onset periodontitis. 25 patients were smokers (> 20 cigarettes/day) and 25 non-smokers. two pooled bacterial samples comprised of four ...	1997	16887581
ribotyping of bacteria from root canal and blood of patients receiving endodontic therapy.	dna restriction patterns and corresponding ribotypes of 26 bacterial isolates (propionibacterium acnes, peptostreptococcus prevotii, fusobacterium nucleatum, prevotella intermedia, prevotella nigrescens, actinomyces israelii, streptococcus intermedius, and streptococcus sanguis) recovered from patients with infected root canals and their peripheral blood, collected during and after endodontic therapy, were examined. eleven additional reference strains including type strains were also examined. p ...	1997	16887597
in vitro activity of linezolid and eperezolid, two novel oxazolidinone antimicrobial agents, against anaerobic bacteria.	linezolid (formerly u-100766) and eperezolid (formerly u-100592) are novel oxazolidinone antimicrobial agents that are active against multi-drug-resistant staphylococci, streptococci, enterococci, corynebacteria, and mycobacteria. preliminary studies also demonstrated that the compounds inhibited some test strains of anaerobic bacteria. therefore, we extended the in vitro evaluation of these agents to include a total of 54 different anaerobic species. minimal inhibitory concentration (mic) value ...	1997	16887605
[bacterial flora in chronic purulent maxillary sinusitis].	in the years from 1993 till 1995 there were 132 tests done on occurrence aerobes (oxygen bacteria) and 56 tests on occurrence of anaerobes and fungus in patients with chronic sinusitis. the most common microorganisms among the aerobes was haemophilus influenzae (23.1%) and staphylococcus aureus (20.9%). the most common microorganisms among the anaerobes was peptococcus and peptostreptococcus (together 57.1%) and from the strains bacteroides (36.8). the breeded oxygen microorganisms gr(+) were mo ...	1997	9757687
immune response to fusobacterium nucleatum, prevotella intermedia and other anaerobes in children with acute tonsillitis.	the number of aerobic and anaerobic bacteria was determined in the saliva of 20 children with acute group a beta-haemolytic streptococcal (gabhs) pharyngo-tonsillitis, and 20 with acute non-gabhs tonsillitis. antibody titres to four gram-negative anaerobic bacilli that reside in the oropharynx (fusobacterium nucleatum, prevotella intermedia, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans) were determined in these and 20 control patients. an average of 8.8 aerobic and anaerobi ...	1997	9222046
time-kill study of the activity of trovafloxacin compared with ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against six anaerobes.	a time-kill method was developed to examine the killing kinetics of trovafloxacin, ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against one strain each of bacteroides fragilis, bacteroides thetaiotaomicron, prevotella melaninogenica, fusobacterium mortiferum, peptostreptococcus magnus and clostridium perfringens. solutions and suspensions were prepared inside an anaerobic glove box, using syringes and prereduced broth. bottles were then incubate ...	1997	9222066
occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings.	bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. the purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. dentin ...	1997	9226388
nmr analysis of the interaction between protein l and ig light chains.	protein l is a cell wall protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. it binds to immunoglobulin (ig) light chains predominantly of the kappa subtype from a wide range of animal species. this binding is mediated by five highly homologous repeats designated as b1-b5, each of which comprises 72 to 76 amino acid residues. the fold of the ig light chain-binding b1 domain of protein l has previously been shown to comprise an alpha-helix packed agains ...	1997	9231896
ovarian vein thrombosis with symptoms prior to term--a case report.	a case with ovarian vein thrombosis before delivery is presented. c-reactive protein exceeded 200 mg/l, and the patient was treated for a suspected infection before the true diagnosis was revealed by ct. after aggressive anti-coagulant therapy, a follow-up ct showed complete remission.	1997	9243212
cloning and expression of the two genes coding for l-serine dehydratase from peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both l-serine dehydratases from escherichia coli.	the structural genes sdha and sdhb, coding for the alpha- and beta-subunits of the [4fe-4s] cluster containing l-serine dehydratase from peptostreptococcus asaccharolyticus, have been cloned and sequenced. expression of modified sdhb together with sdha in escherichia coli led to overproduction of active his6-tagged l-serine dehydratase. e. coli mew22, deficient in the l-serine dehydratase l-sd1, was complemented by this sdhba construct. the derived amino acid sequence of sdhba shares similaritie ...	1997	9244285
separation method of igg fragments using protein l.	protein l (igg kappa-chain-binding bacterial protein) showed a precipitate line(pseudo-immuno-reaction) with igg and f(ab')2 fragment, but did not show any line with the fab fragment, the fc fragment and free kappa-chains in the micro-ouchterlony method. the igg and fab fraction obtained from pa-pain-digested igg (from the sera of patients with chronic thyroiditis), followed by protein a-sepharose, were separated by protein l-sepharose affinity chromatography. the unbound fraction (uf) consisted ...	1997	9246561
microorganisms in vaginal fluid from women in prolonged pregnancy.	in order to compare the vaginal microflora of women in prolonged pregnancy with that of women who delivered at term, samples for quantitative aerobic and anaerobic microbiological culture were collected from 100 women at 42 weeks of gestation and from 60 women at term. the occurrence of lactobacilli-dominated flora was similar in women at term and women with prolonged pregnancy. however, non-hydrogen-peroxide-producing lactobacilli (p < 0.01) were significantly more common and peptostreptococci ...	1997	9251948
beta-lactamase producing bacteria in adult periodontitis.	in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ...	1997	9266340
effect of the addition of peptostreptococcus productus atcc35244 on the gastro-intestinal microbiota and its activity, as simulated in an in vitro simulator of the human gastro-intestinal tract.	peptostreptococcus productus atcc35244, a reductive acetogenic strain, was added daily over 9 successive days to the fourth vessel (ascending colon) of the shime, a six-stage reactor system simulating the in vivo continuous culture conditions of the human gastro-intestinal tract. final numbers of organisms (cfu)/ml reactor contents (c) were attained such that log10c = 6.9 +/- 0.1. the addition caused the ch4 production to decrease below the detection limit while total gas and co2 production in t ...	1997	9274052
use of the isolator 1.5 microbial tube for culture of synovial fluid from patients with septic arthritis.	synovial fluid specimens obtained from patients with arthritis were plated onto solid media (conventional cultures) or inoculated into an isolator 1.5 microbial tube (isolator cultures), and the yield and time to detection of organisms were compared. overall, 144 specimens obtained from 137 patients were processed, and 31 (21.5%) cultures obtained from 29 patients were positive by at least one method. staphylococcus aureus was isolated from 12 patients, streptococcus pneumoniae and kingella king ...	1997	9276427
peptostreptococcal myonecrosis of extraperitoneal origin--a life-threatening complication of pelvic ring disruption.	this report illustrates the clinical and pathological findings of anaerobic streptococcal myonecrosis following extraperitoneal rectal injury in a 28-year-old patient with traumatic pelvic ring disruption. four days following admission, the patient underwent laparotomy, debridement and drainage of the left-sided abdominal wall and presacral space for gross surgical emphysema and streptococcal anaerobic myonecrosis. the specificity of this infection and its impact on the diagnosis and treatment o ...	1997	9283248
increased susceptibility of rag-2 scid mice to dissemination of endodontic infections.	specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. to investigate this question we compared periapical-lesion pathogenesis in rag-2 severe combined immunodeficient (scid) mice with immunocompetent control mice following surgical pulp exposure. in order to equalize the bacterial challenge, an infection protocol ...	1997	9284152
recently described clinically important anaerobic bacteria: taxonomic aspects and update.	a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ...	1997	9310640
dental implant infections.	dental implants provide a restorative tool to support crowns, bridge abutments, and removable dentures. osseointegrated implants are titanium posts that are surgically implanted in alveolar bone. a tight immobile bond (osseointegration) forms between bone and titanium, and prosthetic and restorative fixtures are attached to the implants. titanium implants differ from natural teeth, which may make them more susceptible to mechanical stress. a small proportion of implants are not successful and ma ...	1997	9310682
bilateral anaerobic empyemas complicating infectious mononucleosis.		1997	9315821
in-vitro activity of spiramycin and metronidazole alone or in combination against clinical isolates from odontogenic abscesses.	one hundred and forty-eight isolates of bacteria from 20 intraoral odontogenic abscesses were tested for their susceptibility to spiramycin and metronidazole alone or in combination. all isolates, except rothia spp. (one), enterococcus avium (three), haemophilus parainfluenzae (one) and staphylococcus aureus (one) were sensitive to spiramycin and/or metronidazole. among the anaerobes, spiramycin as well as metronidazole showed good antimicrobial activity against species of prevotella, eubacteriu ...	1997	9338486
the aerobic and anaerobic bacteriology of perirectal abscesses.	the microbiology of perirectal abscesses in 144 patients was studied. aerobic or facultative bacteria only were isolated in 13 (9%) instances, anaerobic bacteria only were isolated in 27 (19%) instances, and mixed aerobic and anaerobic flora were isolated in 104 (72%) instances. a total of 325 anaerobic and 131 aerobic or facultative isolates were recovered (2.2 anaerobic isolates and 0.9 aerobic isolates per specimen). the predominant anaerobes were as follows: bacteroides fragilis group (85 is ...	1997	9350771
prospective study of bacteremia rate after elastic band ligation and sclerotherapy of esophageal varices in patients with hepatosplenic schistosomiasis.	esophageal band ligation is considered to be as efficient as endoscopic sclerotherapy, with a lower complication rate, including bacteremia. there are few studies comparing the two methods. the aim of this study was to compare the incidence of bacteremia after both treatments in patients with portal hypertension secondary to schistosomiasis.	1997	9351034
distribution and mobility of the tetracycline resistance determinant tetq.	we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ...	1997	9372425
metastatic bacterial endophthalmitis. a report of four cases all leading to blindness.	to draw attention to the rare but severe entity of endophthalmitis as encountered due to metastatic spread of bacteria.	1997	9374263
taxonomic update and clinical significance of species within the genus peptostreptococcus.		1997	9376474
coronal leakage of mixed anaerobic bacteria after obturation and post space preparation.	the purpose of this study was to assess bacterial leakage of a mixed anaerobic community of organisms in obturated canals after post space preparation.	1997	9377197
characterization of the free energy spectrum of peptostreptococcal protein l.	native state hydrogen/deuterium exchange studies on cytochrome c and rnase h revealed the presence of excited states with partially formed native structure. we set out to determine whether such excited states are populated for a very small and simple protein, the igg-binding domain of peptostreptococcal protein l.	1997	9377710
[site-specific microbiological tests and the diagnostic significance-- dental and oral infections].		1997	9379527
[antimicrobial activities of clarithromycin against recent obtained clinical isolates].	in order to evaluate antimicrobial activities of clarithromycin (cam), minimum inhibitory concentrations (mics) of cam and control drugs were determined against clinical isolates that were obtained from outpatients in 1994 and 1996. the results are summarized as follows; 1. it was not showed that cam-resistant strains were increasing among staphylococcus spp., beta-streptococci, moraxella subgenus branhamella catarrhalis, haemophilus influenzae, bordetella pertussis, campylobacter jejuni subsp. ...	1997	9394238
[trend of bacterial meningitis in children over a 14 year period (1981 through 1994) in japan--an analysis based on studies in 27 institutions].	we observed 266 children with purulent meningitis in 27 institutions in japan during the 14 years from 1981 on dividing these years into 3 periods, 1981-1985, 1986-1990 and 1991-1994, and studied the trend of causative organisms identified in 254 among the 266 patients. their ages were less than 3 months after birth in 50 children and 3 months or older in 216: there were 141 boys and 125 girls. the causative organisms were h. influenzae in 134 patients and s. pneumoniae in 50, most of them being ...	1997	9394553
evaluation of the bbl crystal anaerobe identification system.	the bbl crystal anaerobe (anr) identification system was evaluated, and the results were compared with those from conventional anaerobic methods. we tested 322 clinically significant anaerobic bacteria according to the manufacturer's instructions. the system identified correctly 286 of 322 (88.8%) of the anaerobic bacteria tested. of these, 263 of 322 (81.7%) were identified correctly on initial testing and 49 were identified correctly only to the genus level; on repeat testing, 23 of 49 (46.9%) ...	1997	9399517
characterization of smooth and rough morphotypes of peptostreptococcus micros.	isolation of the smooth (sm) morphotype of peptostreptococcus micros, a suspected oral pathogen, is sometimes accompanied by isolation of a rough (rg) morphotype of p. micros. the rg type readily changes to a sm-like variant (rgsm) in broth culture. sm and rg isolates and rgsm variants were compared to determine whether these three types are the result of phase variation. the rgsm variants resembled the sm morphotype in colony morphology; furthermore, the sm type and the rgsm type did not have t ...	1997	9103622
cervical necrotizing fasciitis as a complication of acute epiglottitis.		1997	9106089
polymicrobial brain abscess in a patient infected with human immunodeficiency virus.	although intracranial mass lesions that occur as a result of infection have commonly been reported in patients infected with human immunodeficiency virus, polymicrobial pyogenic brain abscess has not been described in this setting. we report the first case of a patient with a polymicrobial brain abscess involving streptococcus bovis, fusobacterium necrophorum, peptostreptococcus, and group g streptococcus, and we review the relevant world literature.	1997	9114198
kinetics of folding of the igg binding domain of peptostreptococcal protein l.	the kinetics of folding of a tryptophan containing mutant of the igg binding domain of protein l were characterized using stopped-flow circular dichroism, stopped-flow fluorescence, and hd exchange coupled with high-resolution mass spectrometry. both the thermodynamics and kinetics of folding fit well to a simple two-state model: (1) guanidine induced equilibrium denaturation transitions measured by fluorescence and circular dichroism were virtually superimposable. (2) the kinetics of folding/un ...	1997	9116017
tissue penetration of meropenem in patients undergoing gynecologic surgery.	the purpose of this study was to assess the tissue-penetrating ability of a new beta-lactam antibiotic, meropenem, in 64 patients undergoing elective gynecologic surgery. patients received a single 500-mg dose intravenously before surgery. plasma and tissue concentrations of meropenem were highest at approximately 1 hour, and good tissue penetration was seen in the variety of specimens evaluated. the median plasma concentration at approximately 1 hour was 13.3 micrograms/ml. the median fluid and ...	1997	9126690
a case of secondary sjögren's syndrome with a swollen thigh.		1997	9135217
antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects.	tosufloxacin, a quinolone, showed a broad antibacterial spectrum against gram-positive and gram-negative bacteria including anaerobic bacteria. tosufloxacin was 4- to 8-fold more active than levofloxacin and ciprofloxacin. the mic90 of tosufloxacin for clinical isolates of bacteroides fragilis, bacteroides vulgatus, bacteroides thetaiotaomicron and peptostreptococcus asaccharolyticus were 0.78, 0.39, 1.56 and 0.39 micrograms/ml, respectively. morphological observation with the scanning and trans ...	1997	9142454
synthesis by an alpha-glucosidase of glycosyl-trehaloses with an isomaltosyl residue.	glycosyl-trehaloses with an isomaltosyl residue were synthesized by alpha-glucosidase from aspergillus niger by using maltotetraose as a glucosyl donor and trehalose as the acceptor. the one trisaccharide and two tetrasaccharides formed were isolated by successive column chromatography. the results of an enzymatic digestion, methylation analysis, and 13c-nmr studies indicated that these oligosaccharides were alpha-isomaltosyl alpha-glucoside, alpha-isomaltotriosyl alpha-glucoside and alpha-isoma ...	1997	9145529
microbiology of chronic maxillary sinusitis: comparison between specimens obtained by sinus endoscopy and by surgical drainage.	the aerobic and anaerobic microbiology of sinus aspirates obtained during surgery was compared with culture of samples obtained by endoscopy. six patients with chronic maxillary sinusitis were evaluated. polymicrobial flora was found in all specimens (two-to-five isolates/sample). a total of 24 isolates (18 anaerobic, five aerobic and one micro-aerophilic) was obtained from sinus aspirates, and 25 isolates (16 anaerobic and nine aerobic) were found in endoscopic specimens. the predominant organi ...	1997	9152041