Publications

exopolysaccharides produced by intestinal bifidobacterium strains act as fermentable substrates for human intestinal bacteria.	eleven exopolysaccharides (eps) isolated from different human intestinal bifidobacterium strains were tested in fecal slurry batch cultures and compared with glucose and the prebiotic inulin for their abilities to act as fermentable substrates for intestinal bacteria. during incubation, the increases in levels of short-chain fatty acids (scfa) were considerably more pronounced in cultures with eps, glucose, and inulin than in controls without carbohydrates added, indicating that the substrates a ...	2008	18539803
biostructure of fecal microbiota in healthy subjects and patients with chronic idiopathic diarrhea.	dysbiosis is a key component of intestinal disorders. our aim was to quantitatively access the biostructure of fecal microbiota in healthy subjects and patients with chronic idiopathic diarrhea and evaluate the responses to saccharomyces boulardii treatment.	2008	18570896
cardiac hypersensitivity to 5-aminosalicylic acid.		2008	2566070
glutamate-immunoreactivity in the retina and optic tectum of goldfish.	glutamate was immunohistochemically localized in the goldfish retina and tectum at the light and electron microscopic (e.m.) levels using double affinity purified antisera against glutaraldehyde conjugated l-glutamate. in retina, glutamate-immunoreactivity (glu+) was observed in cone inner segments, cone pedicles, bipolar cells, a small number of amacrine cells and the majority of cells in the ganglion cell layer. the latter were shown to be ganglion cells by simultaneous retrograde labeling. ce ...	2008	2575431
[tissue-type plasminogen activator and pro-urokinase--new fibrinolytic agents in the treatment of myocardial infarction].	in the light of a survey of the pertinent literature the results of tissue plasminogen activator and pro-urokinase++ use in the thrombolytic treatment of acute myocardial infarction are discussed.	2008	2516932
aerosol application of interferon-alpha in the treatment of bronchioloalveolar carcinoma.	10 patients with locally advanced bronchioloalveolar carcinoma were treated with interferon-alpha as an inhaled aerosol. initial doses ranged between 1 and 10 mu daily or thrice weekly and were then increased to 20 mu daily. treatment was continued until disease progression or excessive toxicity occurred, 9 patients were evaluable for toxicity. in 1 case treatment had to be stopped after 2 weeks due to fever, fatigue and progressive dyspnoea. 2 patients developed fever, 1 had malaise, fatigue an ...	2007	2168195
time to adoption of an innovation by dentists in private practice: sealant utilization.	dentists can be divided into five adoption categories based upon their time of adoption of pit and fissure sealants: innovators, early adopters, early majority, late majority, and laggards. the differences among dentists in the five adoption categories were examined for four classes of variables: practice characteristics, dentist characteristics, communication of information, and practice environment. questionnaires were mailed in september 1984 to a random sample (n = 521) of washington state d ...	2007	1920266
experimental arterial thrombosis in nonhuman primates.	the baboon offers many advantages as an experimental animal to study vascular disease, thrombus formation and dissolution, effects of mediating variables, and the relative efficacy of therapeutic interventions. each specific application for testing therapeutic agents may require testing in somewhat different model systems. for example, although the arteriovenous vascular graft model is efficient, cost effective, and well adapted to study of interventions for acute arterial thrombosis, surgical e ...	2007	2040071
application of 16s rrna gene-targetted fluorescence in situ hybridization and restriction fragment length polymorphism to study porcine microbiota along the gastrointestinal tract in response to different sources of dietary fibre.	a total of 32 pigs of 15+/-0.38 kg body weight were fed for 6 weeks one of four diets differing in their source of dietary fibre. fish was used to quantify the main bacterial groups in the pig gut using the following probes: eub338, bac303, rfla729, rbro730, erec482, fprau645, prop853, str493 and lab158. fish counts revealed important differences at four sites along the pig gastrointestinal tract, but we were unable to show differences related to diets. stomach and jejunal samples gave total bac ...	2007	17004993
16s rrna gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca.	mucosa-associated microbiota from different regions of the gastrointestinal (gi) tract of adult broilers was studied by analysis of 16s rrna gene sequences. the microbiota mainly comprised gram-positive bacteria along the gi tract. fifty-one operational taxonomic units (otus) (from 98 clones) were detected in the ceca, as compared with 13 otus (from 49 clones) in the crops, 11 otus (from 51 clones) in the gizzard, 14 otus (from 52 clones) in the duodenum, 12 otus (from 50 clones) in the jejunum ...	2007	17233749
high proportions of proinflammatory bacteria on the colonic mucosa in a young patient with ulcerative colitis as revealed by cloning and sequencing of 16s rrna genes.	the pathogenesis of ulcerative colitis (uc) remains unknown. it is thought to be due to an abnormal and uncontrolled immune response to normally occurring constituents of the intestine. microbial agents appear to be involved in the pathogenesis and intestinal bacteria seem to be an important factor in the development and chronicity. the aim of this study was to investigate the colonic microbiota of a patient with uc. the colonic tissues were taken during surgery from a 12-year-old girl suffering ...	2007	17265126
quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets.	to explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (agps) on a vegetable-based diet.	2007	17381758
mucosa-associated bacterial diversity in relation to human terminal ileum and colonic biopsy samples.	little is known about bacterial communities that colonize mucosal surfaces in the human gastrointestinal tract, but they are believed to play an important role in host physiology. the objectives of this study were to investigate the compositions of these populations in the distal small bowel and colon. healthy mucosal tissue from either the terminal ileum (n = 6) or ascending (n = 8), transverse (n = 8), or descending colon (n = 4) of 26 patients (age, 68.5 +/- 1.2 years [mean +/- standard devia ...	2007	17890331
influence of bifidobacterium longum bb536 intake on faecal microbiota in individuals with japanese cedar pollinosis during the pollen season.	it has been reported that intake of yogurt or powder supplemented with the bifidobacterium longum bb536 probiotic strain alleviated subjective symptoms and affected blood markers of allergy in individuals with japanese cedar pollinosis (jcpsis) during the pollen seasons of 2004 and 2005, based on randomized, double-blind, placebo-controlled trials. furthermore, the 2004 study found that intestinal bacteria such as the bacteroides fragilis group significantly fluctuated during the pollen season i ...	2007	17893165
imbalance in the composition of the duodenal microbiota of children with coeliac disease.	coeliac disease (cd) is the most common immune-mediated enteropathy characterized by chronic inflammation of the small intestinal mucosa. the ingestion of gluten is responsible for the symptoms of cd, but other environmental factors are also thought to play a role in this disorder. in this study, the composition of the duodenal microbiota of coeliac children with active disease, symptom-free cd patients on a gluten-free diet and control children was determined. bacteriological analyses of duoden ...	2007	18033837
[prenatal ultrasonic diagnosis of developmental abnormalities of the organs of the digestive system].	analysis is made of the results of ultrasound diagnosis of developmental abnormalities of the alimentary tract. it has been established that the information content of echography is dependent on the anatomic intensity of an abnormality and the period of pregnancy at which the examination was done. it has been shown that the method is not of high diagnostic significance in revealing developmental abnormalities of the upper lip and hard palate, atresia of the esophagus, large intestine and rectum. ...	2007	2075063
diffuse neonatal hemangiomatosis: a new constellation of findings.	diffuse neonatal hemangiomatosis is a rare, frequently fatal disease characterized by multiple cutaneous and visceral hemangiomas. the skin, liver, lungs, intestines, and central nervous system are the most commonly affected organs. death can result from high-output cardiac failure as a result of arteriovenous shunting. in this report we present a new constellation of findings in a patient with diffuse neonatal hemangiomatosis, namely, an absent corpus callosum, ectopia cordis (sternal agenesis) ...	2007	1828812
home course in electrocardiography. lesson 3. axis and axis deviation.		2007	1828662
abpi for vein graft surveillance.		2006	1489446
outcome in the chronic fatigue syndrome.		2006	1392899
studies on the effect of system retention time on bacterial populations colonizing a three-stage continuous culture model of the human large gut using fish techniques.	fluorescence in situ hybridization was used to quantitate bacteria growing in a three-stage continuous culture system inoculated with human faeces, operated at two system retention times (60 and 20 h). twenty-three different 16s rrna gene oligonucleotide probes of varying specificities were used to detect bacteria. organisms belonging to genera bacteroides and bifidobacterium, together with the eubacterium rectale/clostridium coccoides group, the atopobium, faecalibacterium prausnitzii and eubac ...	2006	16420637
microbial community composition of the ileum and cecum of broiler chickens as revealed by molecular and culture-based techniques.	the microbial communities of the ileum and cecum of broiler chickens from a conventional and an organic farm were investigated using conventional culture techniques as well as cloning and sequencing of 16s rrna genes. eighty-five percent of the 557 cloned sequences were <97% related to known cultured species. the chicken ileum was dominated by lactobacilli, whereas the cecum harbored a more diverse microbial community. the cecum was dominated by a large group of bacteria with hitherto no close c ...	2006	16830854
molecular profiling of the clostridium leptum subgroup in human fecal microflora by pcr-denaturing gradient gel electrophoresis and clone library analysis.	a group-specific pcr-based denaturing gradient gel electrophoresis (dgge) method was developed and combined with group-specific clone library analysis to investigate the diversity of the clostridium leptum subgroup in human feces. pcr products (length, 239 bp) were amplified using c. leptum cluster-specific primers and were well separated by dgge. the dgge patterns of fecal amplicons from 11 human individuals revealed host-specific profiles; the patterns for fecal samples collected from a child ...	2006	16885270
[the modelling of the development of respiratory muscle force during forced expiration].		2006	1860565
[the modelling of the development of respiratory muscle force during forced expiration].		2006	1860565
uterus didelphys and pregnancy: an unusual case in an 18-year-old patient.	an unusual case of a full-term pregnancy in a patient with a uterus didelphys is presented. the main complication was the formation of pyocolpos in a blind hypoplastic hemivagina observed early in gestation. the therapeutic approach and the related problems are discussed.	2006	1563657
external cephalic version and daily post-versional maternal self-assessment of fetal presentation. a prospective study.	external cephalic version (ecv) using tocolysis is a widely recognized low-risk intervention in cases of breech presentation, particularly in areas where resources are scarce and the perinatal mortality is high in vaginal breech deliveries. in such a setting, pregnant women were trained to make regular self-assessments of the presenting fetal part after ecv in order to detect spontaneous reversions to breech presentation. it was found that such maternal involvement could significantly improve th ...	2006	1563651
structural basis of the subtype selectivity of muscarinic antagonists: a study with chimeric m2/m5 muscarinic receptors.	the five muscarinic receptors (m1-m5), although structurally closely related, can be distinguished pharmacologically by the use of subtype-selective ligands. various tricyclic muscarinic antagonists, including the af-dx derivative aq-ra 741 and the alkaloid himbacine, for example, have been shown to display up to 200-fold higher affinities for m2 and m4 than for m5 receptors. on the other hand, antagonists such as sila-hexocyclium and the pirenzepine derivative uh-ah 37 exhibit lower affinities ...	2006	1538713
sensationalism or sensitivity: use of words in stories on acquired immune deficiency syndrome (aids) by associated press videotext.	although it appeared initially reluctant to cover the story at all, the mainstream press in the united states has supplied almost daily reports on aids since the mid-1980s. the author examined four weeks of stories on the associated press videotext service in early 1986 in an effort to evaluate the validity of critics' charges that journalists were over-emphasizing the role of homosexuals in the progress of the disease, and that their stories were laden with negative or sensationalistic terms. t ...	2006	1856471
[letter: exertion asthma].		2005	1183343
intrathoracic pressure as a tourniquet technic to tamponade bleeding esophageal varices.	the authors' report observations on 79 cases of esophagovariceal bleeding in which ten cases were subjected to an intrathoracic tourniquet maneuver of increasing intrathoracic pressure. all esophagovariceal bleeding ceased following the maneuver. an established maneuver safety limit (holding mean transmural cvp to no higher than 4.0 cm h2o) insured cardiac output maintenance and guaranteed against maneuver-generated morbidity. the authors conclude the cessation of the bleeding from esophageal va ...	2005	1087531
[letter: striae following corticoid therapy].		2005	1169142
seasonal variation of peripheral thyroid hormone levels in major depression.	the seasonal variation in thyroid function tests was examined in 138 patients with major depression. no alterations in thyroxine, free thyroxine index, triiodothyronine, t3 resin uptake and thyrotropin were observed across the four seasons. this applied to both male and female subgroups. these data suggest that seasonality does not account for the wide variability in abnormalities of thyroid function reported in depression.	2005	1774423
treatment of hypertension.		2005	4250702
design and validation of 16s rrna probes to enumerate members of the clostridium leptum subgroup in human faecal microbiota.	among human faecal bacteria, many members of the clostridium leptum subgroup are fibrolytic and butyrate producing microorganisms thereby contributing to processes important to colonic health. yet this phylogenetic subgroup remains poorly described to date. to improve detection and description of members of the c. leptum subgroup, the clep 866 group probe was developed. its association with probes targeting the clostridium viride cluster (cvir 1414) and eubacterium desmolans species (edes 635) a ...	2005	15946290
modulation of the fecal microbiota by the intake of a lactobacillus johnsonii la1-containing product in human volunteers.	lactobacillus johnsonii la1 (la1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. this study evaluates how the ingestion of different amounts of la1 influences the main bacterial populations of the fecal microbiota. eight asymptomatic volunteers participated in the study. after a basal period, they ingested daily 100 ml of a product containing 10(8) cfu ml(-1) of la1 during the first week, 200 ml during the second week a ...	2005	15970400
fecal microbiota composition and frailty.	the relationship between fecal microbiota composition and frailty in the elderly was studied. fecal samples from volunteers with high frailty scores showed a significant reduction in the number of lactobacilli (26-fold). at much higher population levels, both the bacteroides/prevotella (threefold) and the faecalibacterium prausnitzii (fourfold) groups showed a significant reduction in percentage of total number of hybridizable bacteria in the elderly with high frailty scores. in contrast to this ...	2005	16204576
isoflavones and functional foods alter the dominant intestinal microbiota in postmenopausal women.	dietary phytoestrogens, such as isoflavones, are used as food additives to prevent menopause-related disorders. in addition to other factors, their bioavailability strongly depends on the activity of intestinal bacteria but the underlying interactions remain poorly understood. a randomized, double-blind, placebo-controlled study was undertaken with 39 postmenopausal women to characterize changes in the dominant microbial communities of the intestinal tract after 2 mo of isoflavone supplementatio ...	2005	16317121
characterisation of intestinal bacteria in infant stools using real-time pcr and northern hybridisation analyses.	real-time pcr and northern hybridisations were used to quantify bacterial populations in the large gut of infants. pcr primers for rapid, sensitive, high throughput detection of bifidobacteria, bacteroides, sulphate-reducing bacteria and enterococcus faecalis, based on analysis of 16s rrna genes were used. bacterial populations were analysed in faeces from 40 infants aged 0-6, 7-12 and 13-24 months. the effects of breast versus bottle feeding was also investigated. real-time pcr indicated that b ...	2005	16329974
erythema gyratum atrophicans transiens neonatale.	an unknown dermatosis was observed in an infant girl. during the newborn period, she had a generalized, patchy, erythematous eruption of about 60 round erythematous patches on the trunk, thighs, head, labial mucosa, and palate. after a few weeks, the lesions became whitish, atrophic, and depressed, and they were surrounded by an erythematous and slightly infiltrated border, which was occasionally lobulated. histologically, the lesions demonstrated a thin epidermis overlying an edematous dermis. ...	2005	1169042
subdoligranulum variabile gen. nov., sp. nov. from human feces.	during studies on the microflora of human feces we have isolated a strictly anaerobic, non-spore-forming, gram-negative staining organism which exhibits a somewhat variable coccus-shaped morphology. comparative 16s ribosomal rna gene sequencing studies show the unidentified organism is phylogenetically a member of the clostridium leptum supra-generic rrna cluster and displays a close affinity to some rdna clones derived from human and pig feces. the nearest named relatives of the unidentified is ...	2004	16701519
contribution of acetate to butyrate formation by human faecal bacteria.	acetate is normally regarded as an endproduct of anaerobic fermentation, but butyrate-producing bacteria found in the human colon can be net utilisers of acetate. the butyrate formed provides a fuel for epithelial cells of the large intestine and influences colonic health. [1-(13)c]acetate was used to investigate the contribution of exogenous acetate to butyrate formation. faecalibacterium prausnitzii and roseburia spp. grown in the presence of 60 mm-acetate and 10 mm-glucose derived 85-90 % but ...	2004	15182395
characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time pcr and effects of antibiotic treatment on the fecal microbiota.	fecal bacteria were studied in healthy elderly volunteers (age, 63 to 90 years; n = 35) living in the local community, elderly hospitalized patients (age, 66 to 103; n = 38), and elderly hospitalized patients receiving antibiotic treatment (age, 65 to 100; n = 21). group- and species-specific primer sets targeting 16s rrna genes were used to quantitate intestinal bacteria by using dna extracted from feces and real-time pcr. the principal difference between healthy elderly volunteers and both pat ...	2004	15184159
validation of fluorescent in situ hybridization combined with flow cytometry for assessing interindividual variation in the composition of human fecal microflora during long-term storage of samples.	this work was conducted to assess the accuracy of in situ hybridization to show differences in human microflora composition between volunteers and to optimize the storage of fecal samples to allow delayed analysis of gut microflora composition in humans. fecal samples from 25 healthy subjects (14 women, 11 men aged 24-51) were collected. the samples were fixed in 4% paraformaldehyde (pfa) solution at 4 degrees c overnight and stored at -70 degrees c. twenty samples were analysed to quantify the ...	2004	15369862
lactate-utilizing bacteria, isolated from human feces, that produce butyrate as a major fermentation product.	the microbial community of the human colon contains many bacteria that produce lactic acid, but lactate is normally detected only at low concentrations (<5 mm) in feces from healthy individuals. it is not clear, however, which bacteria are mainly responsible for lactate utilization in the human colon. here, bacteria able to utilize lactate and produce butyrate were identified among isolates obtained from 10(-8) dilutions of fecal samples from five different subjects. out of nine such strains ide ...	2004	15466518
rehabilitation counselor and vocational evaluator competencies.		2004	522113
soft tissue damage in war injuries of the upper extremity with special reference to tendons and nerves.		2004	375311
[pressure sensors give a receit for balloon dilatation. do new sensors improve the pacemaker?].		2003	1774977
[combined treatment of rheumatological patients at a health resort].		2003	154775
effects of alternative dietary substrates on competition between human colonic bacteria in an anaerobic fermentor system.	duplicate anaerobic fermentor systems were used to examine changes in a community of human fecal bacteria supplied with different carbohydrate energy sources. a panel of group-specific fluorescent in situ hybridization probes targeting 16s rrna sequences revealed that the fermentors supported growth of a greater proportion of bacteroides and a lower proportion of gram-positive anaerobes related to faecalibacterium prausnitzii, ruminococcus flavefaciens-ruminococcus bromii, eubacterium rectale-cl ...	2003	12571040
oligonucleotide probes that detect quantitatively significant groups of butyrate-producing bacteria in human feces.	16s rrna-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. three new cluster-specific probes detected bacteria related to roseburia intestinalis, faecalibacterium prausnitzii, and eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study ...	2003	12839823
fluorescent hybridisation combined with flow cytometry and hybridisation of total rna to analyse the composition of microbial communities in human faeces using 16s rrna probes.	to determine the structure of human faecal microbiota, faecal samples from 23 healthy individuals were analysed with a similar set of probes targeting six phylogenetic groups using rrna dot-blot hybridisation and whole cell fluorescent in situ hybridisation (fish) combined with flow cytometry. when microbiota compositions derived by each method were compared, the results were not statistically different for clostridium coccoides, fusobacterium prausnitzii, bifidobacterium spp. and enterobacteria ...	2003	19719684
characterization of the glucagon receptor in a pheochromocytoma.	glucagon activated adenylate cyclase in a homogenate of a pheochromocytoma over the concentration range 1 times 10 minus 8m to 1 times 10 minus 6m. several other hormones including adrenocorticotropin, thyrotropin, parathyroid hormone and histamine were without effect. the tumor glucagon receptor was characterized and found to be similar in several ways to the glucagon receptor previously reported in normal tissue such as liver and heart. one, the receptor specifically bound 125-i-glucagon. two, ...	2003	165216
the applications of steroid hormone radioimmunoassays to clinical obstetrics.	this is a selective survey of recent publications dealing with theoretical and established applications of steroid hormone radioimmunoassay procedures applied to clinical obstetric investigation. this subject is reviewed in three sections: first, basic principles of steroid hormone radioimmunoassay methodology; second, steroidogenesis in normal pregnancy and its relation to patterns of steroid hormones measured in maternal and fetal circulations; third, applied steroid radioimmunoassay technolog ...	2002	126392
senator bennett: where are you? any success of p.s.r.o. will be credited to him, any failure to organized medicine.		2002	124080
poncimarin, a new coumarin from poncirus trifoliata l.	a new coumarinic derivative (poncimarin; c19h22o5; m.p. 140 c degrees) was isolated from unripe fruits of poncircus trifoliata l. which from spectroscopic evidences was formulated as 7-(2,3-epoxy-3-methyl butyloxy)-8-(2,3-epoxy-3-methyl butyl) coumarin.	2002	126585
letter: bromide intoxication.		2002	126712
16s-23s rdna internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus fusobacterium.	the 16s-23s rdna internal transcribed spacer (its) regions of all currently defined fusobacterium species and related taxa such as leptotrichia buccalis, sebaldella termitidis and streptobacillus moniliformans, were analysed to examine inter- and intraspecies as well as subspecies relationships. for the its-amplification, a new eubacterial universal primer pair was designed and used. the majority of the fusobacterium strains, along with l. buccalis showed one major, and two to three weaker, dist ...	2002	11931161
diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen.	we reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16s rrna genes. bacteria in the mucosa were highly diverse but mainly gram-positive with low g+c. fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. twenty five percent of the clones had less than 95% homology to database sequences. many sequences were related to those of uncultured bacteria identified in hu ...	2002	11934485
design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples.	an oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ...	2002	12167534
update on the taxonomy and clinical aspects of the genus fusobacterium.	the genus fusobacterium currently includes 13 species. fusobacterium nucleatum, the most frequently encountered species in humans, is heterogeneous and currently includes 5 subspecies. a potentially new subspecies of f. nucleatum that is intrinsically quinolone-resistant and phylogenetically separate from the other 5 subspecies has been identified from dog and cat oral flora. two subspecies have been described for fusobacterium necrophorum, and a new species, fusobacterium equinum, which is rela ...	2002	12173104
acetate utilization and butyryl coenzyme a (coa):acetate-coa transferase in butyrate-producing bacteria from the human large intestine.	seven strains of roseburia sp., faecalibacterium prausnitzii, and coprococcus sp. from the human gut that produce high levels of butyric acid in vitro were studied with respect to key butyrate pathway enzymes and fermentation patterns. strains of roseburia sp. and f. prausnitzii possessed butyryl coenzyme a (coa):acetate-coa transferase and acetate kinase activities, but butyrate kinase activity was not detectable either in growing or in stationary-phase cultures. although unable to use acetate ...	2002	12324374
development of a membrane-array method for the detection of human intestinal bacteria in fecal samples.	a membrane-array method was developed for the detection of human intestinal bacteria in fecal samples without using the expensive microarray-arrayer and laser-scanner. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to nitrocellulose membranes. digoxigenin (dig)-labeled 16s rdnas were amplified by polymerase chain ...	2002	12477438
growth requirements and fermentation products of fusobacterium prausnitzii, and a proposal to reclassify it as faecalibacterium prausnitzii gen. nov., comb. nov.	two newly isolated strains of obligately anaerobic bacteria from human faeces are shown here to be related to fusobacterium prausnitzii, which is regarded as one of the most abundant colonizers of the human colon. these strains, along with fusobacterium prausnitzii atcc 27768(t) and 27766, are non-motile and produce butyrate, formate and lactate, but not hydrogen as fermentation products. a new finding is that all four strains produce d-lactate, but not l-lactate. the strains have a requirement ...	2002	12508881
[studies on the specificity of the antistreptolysin-reaction (author's transl)].		2002	126540
investigation on huntington's disease in the canadian prairies. ii. fecundity and fitness.	the reproductive performance of 157 patients with huntington's disease, their 170 normal siblings and 150 matched unrelated controls is presented. comparison with the general canadian prairie population in the appropriate age group is undertaken. patients with huntington's disease are not only more fecund than their unaffected sibs but also out-reproduce unrelated normal controls and members of the general population of corresponding age. their fitness is thus estimated to be 1.14. should the pr ...	2002	123838
fusobacterium prausnitzii and related species represent a dominant group within the human fecal flora.	the human gut microflora plays a key role in nutrition and health. it has been extensively studied by conventional culture techniques. however these methods are difficult, time consuming and their results not always consistent. furthermore microscopic counts indicate that only 20 to 40% of the total flora can be cultivated. among the predominant species of the human gut, fusobacterium prausnitzii was reported either as one of the most frequent and numerous species or was seldom retrieved. we des ...	2001	11403393
dna isolation protocols affect the detection limit of pcr approaches of bacteria in samples from the human gastrointestinal tract.	a major concern in molecular ecological studies is the lysis efficiency of different bacteria in a complex ecosystem. we used a pcr-based 16s rdna approach to determine the effect of two dna isolation protocols (i.e. the bead beating and triton-x100 method) on the detection limit of seven feces-associated bacterial species of different genera. glycogen was used in these protocols to improve the precipitation of small concentrations of dna in ethanol without affecting the sequential procedures. t ...	2001	11822677
occurrence of the new tetracycline resistance gene tet(w) in bacteria from the human gut.	members of our group recently identified a new tetracycline resistance gene, tet(w), in three genera of rumen obligate anaerobes. here, we show that tet(w) is also present in bacteria isolated from human feces. the tet(w) genes found in human fusobacterium prausnitzii and bifidobacterium longum isolates were more than 99.9% identical to those from a rumen isolate of butyrivibrio fibrisolvens.	2000	10681357
immediate results of weekly fractionation in external radiotherapy.		2000	101487
["information field dental health", an integral part of continuing dental education].		2000	89765
direct analysis of genes encoding 16s rrna from complex communities reveals many novel molecular species within the human gut.	the human intestinal tract harbors a complex microbial ecosystem which plays a key role in nutrition and health. although this microbiota has been studied in great detail by culture techniques, microscopic counts on human feces suggest that 60 to 80% of the observable bacteria cannot be cultivated. using comparative analysis of cloned 16s rrna gene (rdna) sequences, we have investigated the bacterial diversity (both cultivated and noncultivated bacteria) within an adult-male fecal sample. the 28 ...	1999	10543789
phylogenetic analysis of fusobacterium prausnitzii based upon the 16s rrna gene sequence and pcr confirmation.	in order to develop a pcr method to detect fusobacterium prausnitzii in human feces and to clarify the phylogenetic position of this species, its 16s rrna gene sequence was determined. the sequence described in this paper is different from the 16s rrna gene sequence is specific for f. prausnitzii, and the results of this assay confirmed that f. prausnitzii is the most common species in human feces. however, a pcr assay based on the original genbank sequence was negative when it was performed wit ...	1996	8573517
anaerofilum pentosovorans gen. nov., sp. nov., and anaerofilum agile sp. nov., two new, strictly anaerobic, mesophilic, acidogenic bacteria from anaerobic bioreactors.	strictly anaerobic, gram-positive, nonsporing, thin rod-shaped organisms whose cells were 0.2 to 0.6 by 3 to 6 microns were isolated from a hoechst biohochreaktor (strain faet [t = type strain]) and from the biofilm population of a fixed-film reactor treating sour whey (strain ft). strain ft was vigorously motile during early logarithmic growth by means of peritrichously inserted flagella, while strain faet was seldom motile and usually possessed no flagella. during the stationary growth phase b ...	1996	8863411
pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples.	pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ...	1996	8919784
2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay.	xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ...	1992	1563644
thermogenic and hormonal responses to palatable protein and carbohydrate rich food.	simultaneous variations of oxygen consumption, and plasma insulin and norepinephrine were measured during the postprandial cephalic and gastrointestinal phases of feeding in six human subjects following the ingestion of various nutrients. on alternative days the subjects were given foods (1280 kjoules) either rich in carbohydrates (sugar pie) or in proteins (fish). both nutrients produced an initial (0-40 min) enhanced thermogenesis and an early (2 min) cephalic insulin release. during that peri ...	1991	1774019
inactivation of human immunodeficiency virus (hiv) by low ph and pepsin.		1991	1856798
inactivation of human immunodeficiency virus (hiv) by low ph and pepsin.		1991	1856798
differentiation of growth signal requirement of b lymphocyte precursor is directed by expression of immunoglobulin.	during b cell differentiation, at least three stages can be defined in terms of their growth signal requirement by using two different growth signals, which are recombinant interleukin 7 (il-7) and a stromal cell clone pa6 which does not produce il-7; first a pa6 dependent stage, second a pa6 + il-7 dependent stage and third an il-7 dependent stage. in order to test the possibility that this differentiation of growth signal requirement is controlled by the expression of functional immunoglobulin ...	1991	1899373
[new antitubercular drugs].		1990	2168041
protective role of serum antibody in immunity to chlamydial genital infection.	female guinea pigs were injected intraperitoneally with pooled immunoglobulin derived from animals immunized to the chlamydial agent of guinea pig inclusion conjunctivitis. genital infections in animals receiving pooled immunoglobulin from immune animals were markedly decreased with regard to the number of inclusions detected compared with control animals. these data indicated that serum-derived antibody was able to provide a degree of protection against a chlamydial genital tract infection.	1989	2909492
ischaemic mouse thigh model for evaluation of pathogenicity of non-clostridial anaerobes.	a mouse thigh model has been devised in which the growing culture of non-clostridial anaerobe in the ischaemic tissue produces inflammatory swelling and death. the swelling of the right thigh served as an index of pathogenicity of the test strain in comparison to the negatively reacting left thigh which received injection of the control strain of bifidobacterium infantis. actinomyces naeslundii exceptionally caused death in all animals within 24 h. mortality and thigh swelling were pronounced (g ...	1989	2914729
acetylcholine causes nicotinic depolarization in rat dorsal motor nucleus of the vagus, in vitro.	action of acetylcholine (ach) on the electrophysiologically identified preganglionic neurons in the dorsal motor nucleus of the vagus (dmv) were investigated in slice preparations of rat medulla oblongata by using conventional intracellular recording technique. applications of ach to the dmv neurons resulted in a marked depolarization, which was not abolished in ca-free superfusing solution, indicating the direct action of ach on the cells. this depolarization was associated with an increase in ...	1989	2575433
assessing clamp-related vascular injuries by measurement of associated vascular dysfunction.	the development of vascular clamps requires a reliable method to quantitate clamp-related vascular injuries. the degree of vessel damage usually is estimated subjectively from photomicrographs made with scanning electron microscopy. in order to test whether the use of vascular rings to assess residual vascular function may be a better method, rabbit thoracic aortas were occluded by five types of clamps: a fogarty softjaw bulldog, a fogarty hydragrip, a 6-inch satinsky clamp, an edslab bulldog, a ...	1989	2648627
dna alkylation and formation of dna interstrand cross-links by potential antitumour 2,5-bis(1-aziridinyl)-1,4-benzoquinones.	a series of 3,6-substituted 2,5-bis(1-aziridinyl)-1,4-benzoquinone derivatives was shown to alkylate calf thymus dna and to form dna interstrand cross-links. alkylation and cross-link formation were enhanced after electrochemical reduction of the compounds and increased with lower ph in the ph range from 4.5 to 8.0. reduction especially shifts the ph at which cross-linking and alkylation occurs to higher values, which are more physiologically relevant. this shift is probably caused by the increa ...	1989	2663197
[the hormonal response in bulls to the administration of zeranol and rumensin].	the objective of the study was to investigate the influence of the growth stimulators ralgro zeranol and rumensin on changes in concentrations of certain hormones in the blood of beef bulls. in the first experiment 21 bulls were investigated which were divided into three groups. the first group was control, the second was administered the ralgro stimulator (3x) and the third the ralgro and rumensin stimulators. in the second experiment there were 12 bulls. the animals in the test group were impl ...	1989	2667252
minitek urea disk test, a sensitive and cost-effective method to screen for campylobacter pylori in gastric biopsies.	one hundred fifty-five biopsy specimens from the gastric mucosa of 81 patients undergoing routine endoscopy procedures were tested for the presence of campylobacter pylori by three methods: gram stain, culture, and modified minitek, a rapid urea disk test (bbl microbiology systems, cockeysville, md.). twenty-nine patients were infected with c. pylori. sensitivities and specificities of detection were 100 and 94% with the minitek test and 93 and 100% with gram stain, respectively. rapid testing b ...	1989	2687317
falciparum malaria--present day problems. an experience with 425 cases.	clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...	1989	2693436
[immunopathology of glomerulonephritis with mesangial iga deposits].	iga nephropathy is the most frequent type of glomerulonephritis in france. its definition is on renal pathology. mesangial iga deposits are dimeric or polymeric and probably from secretory origin. circulating iga molecules correspond to immune complexes with unknown antigens. the iga subclass remains still controversial: exclusive iga1 versus iga1 and iga2. the pathogenesis of the disease is based on an immune defect specific of the secretory iga system and eventually of the serum iga system. th ...	1989	2699007
use of the glucose oxidase/peroxidase method for glucose assay leads to overestimation of the inhibition of gluconeogenesis by aminopyrine.	4-aminoantipyrine strongly inhibits glucose determination by the glucose oxidase/peroxidase/dianisidine assay but does not interfere in the assay using glucose-6-phosphate dehydrogenase, hexokinase and atp. as a result, the inhibition of gluconeogenesis by aminopyrine reported to be 50-90% (bánhegyi, g., mandl, j., antoni, f. and garzó, t. (1987) biochim. biophys. acta 927, 406-416) is strongly overestimated and amounts to only 10-30%.	1989	2713417
baroreceptor-independent medullary mechanism for release of vasopressin during hypotension in rats.	plasma vasopressin (avp) levels were measured at rest (mean arterial pressure 80-85 mmhg) and during hypotension (mean arterial pressure 38-45 mmhg) induced by ganglionic blockade (trimethaphan) in halothane-anaesthetized respirated rats with end-tidal pco2 maintained at 34-40 mmhg. hypotension (15 min) produced a 310% increase in plasma avp (+/- 60% s.e.m.) which was not reduced significantly by prior baro- and chemoreceptor denervation. the hypotension-induced rise in avp was blocked by bilate ...	1988	2842427
a stable tracer technique for calcium metabolism studies.	a tracer technique using the stable isotope calcium-48 was developed to facilitate studies of human calcium metabolism. small samples of 1 ml serum were used. calcium levels were determined with good precision using neutron-activation analysis after pre-concentration of the serum calcium by oxalate precipitation. the method has sufficient sensitivity to detect induced dilutions of the tracer by administering other less costly isotopes of calcium. the ability to utilize small volumes of sample is ...	1988	2980818
roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo.	fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ...	1988	3044171
[trisomy 13 by translocation. possible prenatal ultrasonic diagnosis].		1988	3057974
the effects of triclabendazole on the lung fluke, paragonimus uterobilateralis in the experimental host sigmodon hispidus.	the new veterinary drug triclabendazole (fasinex) was tested for its anthelmintic activity against adult paragonimus uterobilateralis in experimentally infected cotton rats, sigmodon hispidus. after a single dose of 50, 100 or 150 mg/kg b.w. triclabendazole or after two doses of 5 or 10 mg/kg b.w. given at an interval of 24 hours, 56-61% of the flukes were dead at three to five weeks after treatment. two doses of 50 or 75 mg/kg b.w. triclabendazole killed all flukes. the flukes, which were still ...	1988	3067323
chemotherapy of pulmonary tuberculosis in britain.		1988	3123192
follicle-stimulating hormone induces deoxyribonucleic acid polymerase alpha before deoxyribonucleic acid synthesis in immature rat ovarian follicles perfused in vitro.	to examine the effects of follicle-stimulating hormone (fsh) on cell proliferation and cell division in ovaries, deoxyribonucleic acid (dna) polymerase activities and dna concentrations were determined in ovarian follicles of immature, hypophysectomized, estradiol-treated rats perfused in vitro. ovine fsh (2-20 micrograms/ml) significantly enhanced the dna polymerase alpha activity in the follicles perfused for 180-300 minutes, whereas the dna polymerase beta activity and dna concentration showe ...	1988	3126468
serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1.	aflatoxin-serum albumin adducts in the blood of 42 residents of guangxi province, people's republic of china, were determined and compared with intake of aflatoxin b1 (afb1) and excretion of aflatoxin m1 (afm1) in urine. blood specimens were obtained during the same period that urine was collected and that diet was sampled. serum albumin was isolated from blood by affinity chromatography on reactive blue 2-sepharose and subjected to enzymatic proteolysis using pronase. immunoreactive products we ...	1988	3133131
intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection.	seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ...	1988	3134244