Publications

draft genome sequences of four strains of vibrio parahaemolyticus, three of which cause early mortality syndrome/acute hepatopancreatic necrosis disease in shrimp in china and thailand.	we sequenced four vibrio parahaemolyticus strains, three of which caused serious acute hepatopancreatic necrosis disease. sequence analysis of the virulent strains revealed not only genes related to cholera toxin and the type iv pilus/type iv secretion system but also a unique, previously unreported, large extrachromosomal plasmid that encodes a homolog to the insecticidal photorhabdus insect-related binary toxin pirab.	2014	25189578
identification and expression analysis of immune-related genes linked to rel/nf-κb signaling pathway under stresses and bacterial challenge from the small abalone haliotis diversicolor.	inhibitor of nf-κb (iκb), nuclear factor-κb (nf-κb), and akirin2 are all important members of rel/nf-κb signaling pathway, which plays a pivotal role in regulating the innate immune response of vertebrates and invertebrates. in this study, the iκb (saiκb) and akirin2 (saakirin2) cdnas of small abalone haliotis diversicolor were cloned and characterized. the full length cdna of saiκb and saakirin2 were 1748 bp and 1452 bp respectively, encoding a protein of 401 aa and 187 aa respectively. a conse ...	2014	25158315
regulation of the expression of the vibrio parahaemolyticus peua gene encoding an alternative ferric enterobactin receptor.	a pvsb-vcta-irga triple deletion mutant of vibrio parahaemolyticus can utilize enterobactin under iron-limiting conditions by inducing a previously undescribed receptor, peua (vpa0150), in response to extracellular alkaline ph and enterobactin. in silico analyses revealed the existence of a two-component regulatory system operon, peurs, immediately upstream of peua, which constitutes an operon with the tonb2 system genes. both the peurs and peua-tonb2 operons were found to be upregulated under i ...	2014	25148374
survival of normal and chlorine-stressed pathogenic and non-pathogenic vibrio parahaemolyticus under adverse conditions.	vibrio parahaemolyticus is an important human pathogen which can cause gastroenteritis when consumed in raw or partially-cooked seafood. the pathogenesis of v. parahaemolyticus is based on the presence of virulence factors: the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh), encoded by the tdh and trh genes, respectively.	2014	25147689
detection of acute hepatopancreatic necrosis disease (ahpnd) in mexico.	acute hepatopancreatic necrosis disease (ahpnd), which has also been referred to as early mortality syndrome (ems), initially emerged as a destructive disease of cultured shrimp species in asia in 2009. the pathogen associated with the disease, vibrio parahaemolyticus, subsequently spread to the western hemisphere and emerged in mexico in early 2013. the spread to the western hemisphere is a major concern to shrimp producers in the region. to date, the only peer-reviewed published method for det ...	2014	25144120
performance comparison of second- and third-generation sequencers using a bacterial genome with two chromosomes.	the availability of diverse second- and third-generation sequencing technologies enables the rapid determination of the sequences of bacterial genomes. however, identifying the sequencing technology most suitable for producing a finished genome with multiple chromosomes remains a challenge. we evaluated the abilities of the following three second-generation sequencers: roche 454 gs junior (gs jr), life technologies ion pgm (ion pgm), and illumina miseq (miseq) and a third-generation sequencer, t ...	2014	25142801
remodeling of the intestinal brush border underlies adhesion and virulence of an enteric pathogen.	intestinal colonization by vibrio parahaemolyticus-the most common cause of seafood-borne bacterial enteritis worldwide-induces extensive disruption of intestinal microvilli. in orogastrically infected infant rabbits, reorganization of the apical brush border membrane includes effacement of some microvilli and marked elongation of others. all diarrhea, inflammation, and intestinal pathology associated with v. parahaemolyticus infection are dependent upon one of its type 3 secretion systems (t3ss ...	2014	25139905
high-quality draft genomes of two vibrio parahaemolyticus strains aid in understanding acute hepatopancreatic necrosis disease of cultured shrimps in mexico.	the high-quality draft genomes of two vibrio parahaemolyticus strains, one that causes the acute hepatopancreatic necrosis disease (ahpnd) in cultured shrimps (fim-s1708(+)), and another that does not (fim-s1392(-)) are reported. a chromosome-scale assembly for the fim-s1392(-) genome is reported here. the analysis of the two genomes gives some clues regarding the genomic differences between the strains.	2014	25125645
complete genome sequence of virulent bacteriophage shou24, which infects foodborne pathogenic vibrio parahaemolyticus.	a novel lytic vibrio parahaemolyticus phage (shou24) belonging to the family siphoviridae was isolated from aquatic market sewage. the phage is only able to infect v. parahaemolyticus containing a tdh gene. shou24 has a linear genome of 77,837 bp with a g+c content of 46.0 %. in total, 88 predicted proteins have homologues in databases, and the majority of the core genes share high sequence similarity with genes from unrelated viruses and bacteria. genes related to lysogeny and host lysis were n ...	2014	25115946
phylogenetic and in silico functional analyses of thermostable-direct hemolysin and tdh-related encoding genes in vibrio parahaemolyticus and other gram-negative bacteria.	emergence and spread of pandemic strains of vibrio parahaemolyticus have drawn attention to make detailed study on their genomes. the pathogenicity of v. parahaemolyticus has been associated with thermostable-direct hemolysin (tdh) and/or tdh-related hemolysin (trh). the present study evaluated characteristics of tdh and trh genes, considering the phylogenetic and in silico functional features of v. parahaemolyticus and other bacteria. fifty-two tdh and trh genes submitted to the genbank were an ...	2014	25114910
snapshot of vibrio parahaemolyticus densities in open and closed shellfish beds in coastal south carolina and mississippi.	vibrio parahaemolyticus is a gram negative, halophilic bacterium that is ubiquitous in warm, tropical waters throughout the world. it is a major cause of seafood-associated gastroenteritis and is generally associated with consumption of raw or undercooked seafood, especially oysters. this study presents a snapshot of total v. parahaemolyticus densities in surface waters and shellstock american oysters (crassostrea virginica) from open and closed shellfish harvesting areas, as well as "more rural ...	2014	25106119
draft genome sequences of clinical vibrio parahaemolyticus strains isolated in maryland (2010 to 2013).	vibrio parahaemolyticus is the leading cause of food-borne illnesses associated with the consumption of raw shellfish worldwide. here, we report 45 draft genomes of v. parahaemolyticus. thirty-five of them are strains that were isolated from clinical cases in the state of maryland from 2010 to 2013. the remaining 10 strains were historical isolates, isolated mostly from the west coast of the united states during the period of 1988 to 2004. the availability of these genomes will allow for future ...	2014	25103764
eating oysters without risk of vibriosis: application of a bacteriophage against vibrio parahaemolyticus in oysters.	vibrio parahaemolyticus is a major cause of foodborne illness and related with the consumption of raw contaminated seafood, especially oysters. to evaluate the effectiveness of various applications of a bacteriophage (phage), pvp-1, against a multiple-antibiotic-resistant v. parahaemolyticus pandemic strain (crs 09-17), we designed artificial contamination models that are most likely to be encountered during oyster processing. when live oysters were treated with bath immersion with pvp-1 after c ...	2014	25086350
impact of seafood regulations for vibrio parahaemolyticus infection and verification by analyses of seafood contamination and infection.	consumption of seafood contaminated with vibrio parahaemolyticus causes foodborne infections, which are on the rise owing to increased consumption of raw seafood in asia, europe, north america, and other regions. v. parahaemolyticus infections have been common in japan since the 1960s. following an epidemic in 1997, the japanese ministry of health, labour, and welfare instituted regulations for seafood in 1999, which appear to be reducing v. parahaemolyticus infections. in this review, we descri ...	2014	25078437
occurrence of potentially pathogenic vibrio in oysters (crassostrea gigas) and waters from bivalve mollusk cultivations in the south bay of santa catarina.	this research aimed to identify and quantify potentially pathogenic vibrio from different cultivations of bivalve shellfish in the state of santa catarina, brazil, and water regions in the south bay, as well as correlate the incidence of these microorganisms with the physicochemical parameters of marine waters.	2014	25075484
prevalence and quantification of pathogenic vibrio parahaemolyticus during shrimp culture in thailand.	vibrio parahaemolyticus is a major cause of seafood-borne gastroenteritis. the human pathogenic strains possess tdh or trh or both genes. in thai shrimp farming, the level of pathogenic v. parahaemolyticus contamination has not been completely characterized, although it has been identified as a risk for people who consume undercooked shrimp. in this study, the prevalence and concentration of v. parahaemolyticus (total vp) and pathogenic v. parahaemolyticus (tdh+ vp and trh+ vp) were investigated ...	2014	25449321
insights into vibrio parahaemolyticus chn25 response to artificial gastric fluid stress by transcriptomic analysis.	vibrio parahaemolyticus is the causative agent of food-borne gastroenteritis disease. once consumed, human acid gastric fluid is perhaps one of the most important environmental stresses imposed on the bacterium. herein, for the first time, we investigated vibrio parahaemolyticus chn25 response to artificial gastric fluid (agf) stress by transcriptomic analysis. the bacterium at logarithmic growth phase (lgp) displayed lower survival rates than that at stationary growth phase (sgp) under a sub-le ...	2014	25490137
transcriptome and expression profiling analysis of the hemocytes reveals a large number of immune-related genes in mud crab scylla paramamosain during vibrio parahaemolyticus infection.	mud crab scylla paramamosain is an economically important marine species in china. however, frequent outbreaks of infectious diseases caused by marine bacteria, such as vibrio parahaemolyticus, result in great economic losses.	2014	25486443
metadynamics simulations reveal a na+ independent exiting path of galactose for the inward-facing conformation of vsglt.	sodium-galactose transporter (sglt) is a secondary active symporter which accumulates sugars into cells by using the electrochemical gradient of na+ across the membrane. previous computational studies provided insights into the release process of the two ligands (galactose and sodium ion) into the cytoplasm from the inward-facing conformation of vibrio parahaemolyticus sodium/galactose transporter (vsglt). several aspects of the transport mechanism of this symporter remain to be clarified: (i) a ...	2014	25522004
comparative genomic analysis of clinical and environmental strains provides insight into the pathogenicity and evolution of vibrio parahaemolyticus.	vibrio parahaemolyticus is a gram-negative halophilic bacterium. infections with the bacterium could become systemic and can be life-threatening to immunocompromised individuals. genome sequences of a few clinical isolates of v. parahaemolyticus are currently available, but the genome dynamics across the species and virulence potential of environmental strains on a genome-scale have not been described before.	2014	25518728
vibrio parahaemolyticus strengthens their virulence through modulation of cellular reactive oxygen species in vitro.	vibrio parahaemolyticus (vp) is one of the emergent food-borne pathogens that are commensally associated with various shellfish species throughout the world. it is strictly environmental and many strains are pathogenic to humans. the virulent strains cause distinct diseases, including wound infections, septicemia, and most commonly, acute gastroenteritis, which is acquired through the consumption of raw or undercooked seafood, especially shellfish. vp has two type three secretion systems (t3sss) ...	2014	25566508
h-ns is a repressor of major virulence gene loci in vibrio parahaemolyticus.	vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci t3ss1, vp-pai (t3ss1+tdh2) and t6ss2. as showing in this study, the nucleoid-associated dna-binding regulator h-ns binds to multiple promoter-proximal regions in each of the above three loci to repress their transcription, and moreover h-ns inhibits the cytotoxicitiy, enterotoxicity, hemolytic activity, and mouse lethality of v. parahaemolyticus. h-ns appears to ac ...	2014	25566201
vibrio parahaemolyticus: a rare cause of chronic diarrhea in a heart transplant patient.	vibrio parahaemolyticus usually causes a self-limiting acute diarrheal illness, and is rarely tested for in cases of chronic diarrhea. we present a rare case of chronic diarrhea caused by v. parahaemolyticus in a heart transplant patient requiring antibiotic treatment.	2014	26157876
incidence of multidrug resistant vibrio parahaemolyticus isolated from ponnani, south india.	the prevalence of vibrio parahaemolyticus has been reported from ponnani earlier, however incidence of multidrug resistant strains have been encountered recently in clinical laboratories. the source for such strains and their presence in this major fish landing centre has been investigated.	2014	25705353
roles of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) in vibrio parahaemolyticus.	vibrio parahaemolyticus is the leading cause of seafood borne bacterial gastroenteritis in the world, often associated with the consumption of raw or undercooked seafood. however, not all strains of v. parahaemolyticus are pathogenic. the thermostable direct hemolysin (tdh) or tdh-related hemolysin (trh) encoded by tdh and trh genes, respectively, are considered major virulence factors in v. parahaemolyticus. however, about 10% of clinical strains do not contain tdh and/or trh. environmental iso ...	2014	25657643
[etiologic characteristics of vibrio parahaemolyticus strains causing outbreaks and sporadic cases in guangdong, 2013].	to study the serotypes, antimicrobial resistance, virulence genes and molecular characteristics of vibrio parahaemolyticus isolated from outbreaks and sporadic cases in guangdong, 2013.	2014	25623459
[vibrio spp. infections of clinical significance and implication for public health].	vibrio spp. infections still are a public health concern. vibrio spp. can be found in marine, estuarine, and freshwater environments, and can be able to cause diseases in fish, shellfish, mammals, as well as in humans. since '80 to date, the number of species within the genus increased from 21 to more than 100. the most important is vibrio cholerae, the etiological agent of the cholera, responsible of seven pandemics; serotypes o1 and o139 can produce cholera toxin, while serotypes non-o1/non-o1 ...	2014	22670341
purification, characterization and production optimization of a vibriocin produced by mangrove associated vibrio parahaemolyticus.	to identify a potential bacterium which produces antimicrobial peptide (vibriocin), and its purification, characterization and production optimization. the bacteria subjected in the study were isolated from a highly competitive ecological niche of mangrove ecosystem.	2014	25182547
probiotic supplementation influences the diversity of the intestinal microbiota during early stages of farmed senegalese sole (solea senegalensis, kaup 1858).	ingestion of bacteria at early stages results in establishment of a primary intestinal microbiota which likely undergoes several stages along fish life. the role of this intestinal microbiota regulating body functions is crucial for larval development. probiotics have been proved to modulate this microbiota and exert antagonistic effects against fish pathogens. in the present study, we aimed to determine bacterial diversity along different developmental stages of farmed senegalese sole (solea se ...	2014	25103323
iron shortage and bile salts play a major role in the expression of ompk gene in vibrio anguillarum.	the outer membrane protein k, ompk first identified in vibrio parahaemolyticus has been shown to be a receptor for a broad host range vibriophage kvp40 infecting members of the vibrionaceae. in the study, the effect of culture conditions on the expression of ompk in v. anguillarum was studied using real-time pcr. the expression increased significantly in the presence of bile salts and iron chelating agent 2, 2' bipyridine, suggesting a role for this protein in bile resistance and also in iron ac ...	2014	25033659
abundance of vibrio cholerae, v. vulnificus, and v. parahaemolyticus in oysters (crassostrea virginica) and clams (mercenaria mercenaria) from long island sound.	vibriosis is a leading cause of seafood-associated morbidity and mortality in the united states. typically associated with consumption of raw or undercooked oysters, vibriosis associated with clam consumption is increasingly being reported. however, little is known about the prevalence of vibrio spp. in clams. the objective of this study was to compare the levels of vibrio cholerae, vibrio vulnificus, and vibrio parahaemolyticus in oysters and clams harvested concurrently from long island sound ...	2014	25281373
identification of capsule, biofilm, lateral flagellum, and type iv pili in vibrio mimicus strains.	vibrio mimicus is a bacterium that causes gastroenteritis; it is closely related to vibrio cholerae, and can cause acute diarrhea like cholera- or dysentery-type diarrhea. it is distributed worldwide. factors associated with virulence (such as hemolysins, enterotoxins, proteases, phospholipases, aerobactin, and hemagglutinin) have been identified; however, its pathogenicity mechanism is still unknown. in pathogenic vibrio species such as v. cholerae, vibrio. parahaemolyticus and vibrio vulnificu ...	2014	25246027
pathogenic vibrios in environmental, seafood and clinical sources in germany.	bacteria of the family vibrionaceae naturally occur in marine and estuarine environments. only few species of vibrionaceae are associated with human cases of gastroenteritis, ear and wound infections, caused by ingestion of seafood or contact with vibrio containing water. increasing consumption of seafood (fish, fishery products and shellfish) poses a possible source of vibrio infections in germany. additionally, there is a growing concern that abundances of pathogenic vibrios may increase in ge ...	2014	25129553
occurrence of the three major vibrio species pathogenic for human in seafood products consumed in france using real-time pcr.	vibrio spp. have emerged as a serious threat to human health worldwide. vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus are of particular concern as they have been linked to gastrointestinal infections and septicemia associated with the consumption of raw or undercooked seafood. we developed hydrolysis probe-based real-time pcr systems with an internal amplification control for the detection of these species. we applied these systems to a total of 167 fresh or frozen crustacean, f ...	2014	25128747
γ-hydroxynitrile glucosides from the seeds of prinsepia utilis.	γ-hydroxynitrile glucosides (prinsepicyanosides a-e) were isolated alongside 11 known compounds from seeds of prinsepia utilis royle. their structures were determined by detailed analysis of nmr and ms spectroscopic data. the relative configuration of prinsepicyanoside c was established by cu-kα x-ray crystallography. prinsepicyanoside a, osmaronin, and 4-(hydroxylmethyl)-5h-furan-2-one exhibited borderline antibacterial activity against salmonella gallinarum, vibrio parahaemolyticus, and vibrio ...	2014	24952469
high prevalence of toxin producing enteropathogenic vibrios among estuarine crab in ganges delta of west bengal, india.		2014	24927645
sequence and expression divergence of an ancient duplication of the chaperonin groesel operon in vibrio species.	heat-shock proteins are molecular chaperones essential for protein folding, degradation and trafficking. the human pathogen vibrio vulnificus encodes a copy of the groesel operon in both chromosomes and these genes share <80 % similarity with each other. comparative genomic analysis was used to determine whether this duplication is prevalent among vibrionaceae specifically or gammaproteobacteria in general. among the vibrionaceae complete genome sequences in the database (31 species), seven vibr ...	2014	24913685
high-salt preadaptation of vibrio parahaemolyticus enhances survival in response to lethal environmental stresses.	adaptation to changing environmental conditions is an important strategy for survival of foodborne bacterial pathogens. vibrio parahaemolyticus is a gram-negative seafoodborne enteric pathogen found in the marine environment both free living and associated with oysters. this pathogen is a moderate halophile, with optimal growth at 3% nacl. among the several stresses imposed upon enteric bacteria, acid stress is perhaps one of the most important. v. parahaemolyticus has a lysine decarboxylase sys ...	2014	24490918
occurrence of virulence genes among vibrio cholerae and vibrio parahaemolyticus strains from treated wastewaters.	pathogenic vibrio species are an important cause of foodborne illnesses. the aim of this study was to describe the occurrence of potentially pathogenic vibrio species in the final effluents of a wastewater treatment plant and the risk that they may pose to public health. during the 1-year monitoring, a total of 43 vibrio strains were isolated: 23 vibrio alginolyticus, 1 vibrio cholerae, 4 vibrio vulnificus, and 15 vibrio parahaemolyticus. the pcr investigation of v. parahaemolyticus and v. chole ...	2014	25023745
multiplex pcr assays for the detection of vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae with an internal amplification control.	a multiplex polymerase chain reaction (pcr) assay that can simultaneously detect 4 major vibrio spp., vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae, in the presence of an internal amplification control (iac) was developed. species-specific pcr primers were designed based on the gyrb gene for v. alginolyticus, the collagenase gene for v. parahaemolyticus, the vvha gene for v. vulnificus, and the ompw gene for v. cholerae. additionally, an iac primer pair wa ...	2014	24731836
vibrio parahaemolyticus and its specific bacteriophages as an indicator in cockles (anadara granosa) for the risk of v. parahaemolyticus infection in southern thailand.	correlation between the numbers of vibrio parahaemolyticus and its specific bacteriophages in cockles was investigated from june 2009 to may 2010 in hat yai, songkhla, thailand. cockles obtained monthly from a local market were sampled to determine the numbers of v. parahaemolyticus and bacteriophages that could form plaques on ten strains of pandemic and nonpandemic v. parahaemolyticus. in addition, v. parahaemolyticus isolates from clinical samples from hat yai hospital over the same period we ...	2014	24682339
detection of vibrio parahaemolyticus, vibrio vulnificus and vibrio cholerae with respect to seasonal fluctuations in temperature and plankton abundance.	over a 1-year period, bi-monthly estuarine surface water and plankton samples (63-200 and > 200 μm fractions) were assayed by polymerase chain reaction for the prevalence of total vibrio parahaemolyticus, v. vulnificus and v. cholerae and select genes associated with clinical strains found in each species. neither temperature nor plankton abundance was a significant correlate of total v. parahaemolyticus; however, the prevalence of genes commonly associated with clinical strains (trh, tdh, orf8) ...	2014	24024909
surface expression of protein a on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes.	magnetosomes are membrane-enclosed magnetite nanocrystals synthesized by magnetotactic bacteria (mtb). they display chemical purity, narrow size ranges, and species-specific crystal morphologies. specific transmembrane proteins are sorted to the magnetosome membrane (mm). mamc is the most abundant mm protein of magnetospirillum gryphiswaldense strain msr-1. mamf is the second most abundant mm protein of msr-1 and forms stable oligomers. we expressed staphylococcal protein a (spa), an immunoglobu ...	2014	24765089
pellino protein from pacific white shrimp litopenaeus vannamei positively regulates nf-κb activation.	pellino, named after its property that binds pelle (the drosophila melanogaster homolog of irak1), is a highly conserved e3 class ubiquitin ligase in both vertebrates and invertebrates. pellino interacts with phosphorylated irak1, causing polyubiquitination of irak1, and plays a critical upstream role in the toll-like receptor (tlr) pathway. in this study, we firstly cloned and identified a crustacean pellino from pacific white shrimp litopenaeus vannamei (lvpellino). lvpellino contains a putati ...	2014	24463313
molecular characterization and functional analysis of cactin gene from litopenaeus vannamei.	cactin (cactus interactor) is a conserved protein which was initially discovered as a novel interactor of drosophila ikb protein cactus. cactin was always characterized as a negative regulator of many different developmental processes, but only found to play an immune role in humans. to better know the immune function of cactin gene, litopenaeus vannamei cactin (lvcactin) was identified and characterized in this study. the full-length cdna of lvcactin is 3, 150 bp long, with an open reading fram ...	2014	25462455
genes similar to the vibrio parahaemolyticus virulence-related genes tdh, tlh, and vscc2 occur in other vibrionaceae species isolated from a pristine estuary.	detection of the human pathogen vibrio parahaemolyticus often relies on molecular biological analysis of species-specific virulence factor genes. these genes have been employed in determinations of v. parahaemolyticus population numbers and the prevalence of pathogenic v. parahaemolyticus strains. strains of the vibrionaceae species photobacterium damselae, vibrio diabolicus, vibrio harveyi, and vibrio natriegens, as well as strains similar to vibrio tubiashii, were isolated from a pristine salt ...	2014	24212573
development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals.	rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ...	2014	24954661
antibacterial effect of silver nanoparticles against four foodborne pathogens.	there is increased demand for improved disinfection methods due to microorganisms resistant to multiple antimicrobial agents. numerous types of disinfectants are available with different properties; but the proper disinfectant must be carefully selected for any specific application to obtain the desired antimicrobial effect.	2014	25147658
detection and identification of six foodborne bacteria by two-tube multiplex real time pcr and melting curve analysis.	this study is aimed to develop a two-tube melting curve-based multiplex real time pcr assay (mcmrt-pcr) for the simultaneous detection of six common foodborne pathogenic bacteria (diarrhoeagenic escherichia coli, salmonella, and shigella in tube 1, staphylococcus aureus, vibrio parahaemolyticus, and listeria monocytogenes in tube 2).	2014	25341812
a novel, broad-range, ctxφ-derived stable integrative expression vector for functional studies.	ctxφ, a filamentous vibriophage encoding cholera toxin, uses a unique strategy for its lysogeny. the single-stranded phage genome forms intramolecular base-pairing interactions between two inversely oriented xerc and xerd binding sites (xbs) and generates a functional phage attachment site, attp(+), for integration. the attp(+) structure is recognized by the host-encoded tyrosine recombinases xerc and xerd (xercd), which enables irreversible integration of ctxφ into the chromosome dimer resoluti ...	2014	25225263
occurrence of vibrio and salmonella species in mussels (mytilus galloprovincialis) collected along the moroccan atlantic coast.	this study reports the occurrence of different vibrio and salmonella species in 52 samples of mytilus galloprovincialis collected from four sites along the atlantic coast between agadir and essaouira (anza, cap ghir, imssouane and essaouira). the level of escherichia coli (e. coli) was also determined to evaluate the degree of microbial pollution in the investigated areas. in this study three methods were used : afnor nf en iso 6579 v08-013 for salmonella spp., the provisional method routinely u ...	2014	24936389
alternative sigma factor rpoe is important for vibrio parahaemolyticus cell envelope stress response and intestinal colonization.	vibrio parahaemolyticus is a halophile that inhabits brackish waters and a wide range of hosts, including crustaceans, fish, mollusks, and humans. in humans, it is the leading cause of bacterial seafood-borne gastroenteritis. the focus of this work was to determine the role of alternative sigma factors in the stress response of v. parahaemolyticus rimd2210633, an o3:k6 pandemic isolate. bioinformatics identified five putative extracytoplasmic function (ecf) family of alternative sigma factors: v ...	2014	24935982
detection and quantification of vibrio parahaemolyticus in shellfish from italian production areas.	vibrio parahaemolyticus is a marine microorganism, recognized as an important cause of foodborne illness particularly in asia, south america and united states. outbreaks are rarely reported in europe, but they can occur unexpectedly in relation, among other reasons, to the spread of highly virulent strains. it is known that the risk is proportional to exposure levels to pathogenic v. parahaemolyticus (i.e. carrying the tdh and/or the trh genes) but currently there is a lack of occurrence data fo ...	2014	24810197
plasmid-related quinolone resistance determinants in epidemic vibrio parahaemolyticus, uropathogenic escherichia coli, and marine bacteria from an aquaculture area in chile.	marine bacteria from aquaculture areas with industrial use of quinolones have the potential to pass quinolone resistance genes to animal and human pathogens. the vpa0095 gene, related to the quinolone resistance determinant qnra, from clinical isolates of epidemic vibrio parahaemolyticus conferred reduced susceptibility to quinolone after cloning into escherichia coli k-12 either when acting alone or synergistically with dna gyrase mutations. in addition, a plasmid-mediated quinolone resistance ...	2014	24760167
characterization of putative toxin/antitoxin systems in vibrio parahaemolyticus.	to obtain more information about the toxin/antitoxin (ta) systems in the vibrio genus and also to examine their involvement in the induction of a viable but nonculturable (vbnc) state, we searched homologues of the escherichia coli ta systems in the vibrio parahaemolyticus genome.	2014	24698443
reassessing provider reporting in the age of electronic surveillance.	in 2000, the centers for disease control and prevention began funding health departments to implement integrated electronic systems for disease surveillance.	2014	24458313
vibrio parahaemolyticus enolase is an adhesion-related factor that binds plasminogen and functions as a protective antigen.	vibrio parahaemolyticus, an emerging food and waterborne pathogen, is a leading cause of seafood poisoning worldwide. surface proteins can directly participate in microbial virulence by facilitating pathogen dissemination via interactions with host factors. screening and identification of protective antigens is important for developing therapies against v. parahaemolyticus infections. here, we systematically characterized a novel immunogenic enolase of v. parahaemolyticus. the enolase gene of v. ...	2014	24430205
effect of sypq gene on poly-n-acetylglucosamine biosynthesis in vibrio parahaemolyticus and its role in infection process.	the syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. however, the individual functions of the respective genes remain unclear. amino acid alignment indicates that sypq is presumably involved in biosynthesizing poly-n-acetylglucosamine (pnag), which is proposed to be a critical virulence factor ...	2014	24418930
pvv3, a new shuttle vector for gene expression in vibrio vulnificus.	an efficient electroporation procedure for vibrio vulnificus was designed using the new cloning vector pvv3 (3,107 bp). transformation efficiencies up to 2 × 10(6) transformants per μg dna were achieved. the vector stably replicated in both v. vulnificus and escherichia coli and was also successfully introduced into vibrio parahaemolyticus and vibrio cholerae. to demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (gfp) gene and the vvhba hemolysin oper ...	2014	24362421
cloning and identification of a novel nhad-type na+/h+ antiporter from metagenomic dna of the halophilic bacteria in soil samples around daban salt lake.	in this study, metagenomic dna was screened for the na(+)/h(+) antiporter gene from the halophilic bacteria in daban salt lake by selection in escherichia coli knabc lacking three major na(+)/h(+) antiporters. one gene designated as hb_nhad encoding a novel nhad-type na(+)/h(+) antiporter was finally cloned. the presence of hb_nhad conferred tolerance of e. coli knabc to up to 0.5 m nacl and 0.2 m licl, and an alkaline ph. hb_nhad has the highest identity (70.6%) with a putative nhad-type na(+)/ ...	2014	24297704
antimicrobial effect of bayberry leaf extract for the preservation of large yellow croaker (pseudosciaena crocea).	chemical preservatives have been widely used to keep large yellow croaker fresh. however, the potential harm to human health cannot be ignored. this study was undertaken to investigate the antimicrobial effect of bayberry leaf extract and to evaluate the efficacy of this natural product on the preservation of large yellow croaker.	2014	23929386
depuration times of donax trunculus and tapes decussatus.	the present study was performed to determine the depuration time and ability of donax trunculus (wedge clam) and tapes decussatus (carpet shell) contaminated with escherichia coli, salmonella enterica subsp. enterica serovar typhimurium and vibrio parahaemolyticus. clams were contaminated with each bacterium at the level of 7.0 - 8.0 log10 cfu/g. after contamination, clams were analyzed every 3 h in the first 24 h time period and every 6 h until the 72nd hour. during the depuration process of bo ...	2014	25477938
efficacy of neutral electrolyzed water for reducing pathogenic bacteria contaminating shrimp.	pathogenic contamination is a food safety concern. this study was conducted to investigate the efficacy of neutral electrolyzed water (new) in killing pathogens, namely, vibrio parahaemolyticus, vibrio vulnificus, salmonella enteritidis, and escherichia coli in shrimp. pure cultures of each pathogen were submerged separately in new containing five different chlorine concentrations: 10, 30, 50, 70, and 100 ppm. for each concentration, three submersion times were tested: 1, 3, and 5 min. the popul ...	2014	25474069
[purification and dna-binding of toxr truncated protein of vibrio parahaemolyticus].	the dna-binding domain of toxr protein of vibrio parahaemolyticus was expressed using the escherichia coli bl21lambdade3 protein expression system, and its dna-binding activity was characterized.	2014	25345029
immunological function of familial mediterranean fever disease protein pyrin.	pyrin, encoded by mefv gene, is conserved in humans and mice. mutations in the mefv gene are associated with the human autoinflammatory disease familial mediterranean fever (fmf). pyrin can interact with the inflammasome adaptor asc and induce inflammatory caspase-1 activation in monocytic cells, but the physiological function of pyrin has been unknown for many years. here we summarize previous studies of pyrin function under the context of fmf and immunity, and discuss a recent study demonstrat ...	2014	25307949
innate immune sensing of bacterial modifications of rho gtpases by the pyrin inflammasome.	cytosolic inflammasome complexes mediated by a pattern recognition receptor (prr) defend against pathogen infection by activating caspase 1. pyrin, a candidate prr, can bind to the inflammasome adaptor asc to form a caspase 1-activating complex. mutations in the pyrin-encoding gene, mefv, cause a human autoinflammatory disease known as familial mediterranean fever. despite important roles in immunity and disease, the physiological function of pyrin remains unknown. here we show that pyrin mediat ...	2014	24919149
disease-outcome trees, eq-5d scores, and estimated annual losses of quality-adjusted life years (qalys) for 14 foodborne pathogens in the united states.	measures of disease burden such as quality-adjusted life years (qalys) are increasingly important to risk-based food safety policy. they provide a means of comparing relative risk from diverse health outcomes. we present detailed disease-outcome trees and eq-5d scoring for 14 major foodborne pathogens representing over 95% of foodborne illnesses, hospitalizations, and deaths due to specified agents in the united states (campylobacter spp., clostridium perfringens, cryptosporidium parvum, cyclosp ...	2014	24588853
a modified molecular beacons-based multiplex real-time pcr assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application.	foodborne disease outbreaks are often caused by one of the major pathogens. early identification of the causal pathogen is crucial for disease control and prevention. we describe a real-time polymerase chain reaction (rtpcr) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including salmonella enterica subsp. enterica, listeria monocytogenes, escherichia coli o157, vibrio parahaemolyticus, v. vulnificus, campylobacter jejuni, enterobacter sakazakii ...	2014	24328501
antimicrobial function of shβap, a novel hemoglobin β chain-related antimicrobial peptide, isolated from the liver of skipjack tuna, katsuwonus pelamis.	a 2.3 kda of antimicrobial peptide was purified from an acidified liver extract of skipjack tuna, katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and c18 reversed-phase hplc. a comparison of the amino acid sequence of the purified peptide with those of other known polypeptides revealed high homology with the c-terminus of hemoglobin β-chain; thus, this peptide was designated as the skipjack hemoglobin β chain-related antimicrobial peptide (shβap). shβap showed pot ...	2014	24495783
ptlgbp, a pattern recognition receptor in portunus trituberculatus involved in the immune response against different challenges.	lipopolysaccharide and b-1,3-glucan binding protein (lgbp) is a pattern recognition receptor that can recognize and bind lps and b-1,3-glucan. lgbp has crucial roles in innate immune defense against gram-negative bacteria and fungi. in this study, lgbp functions in portunus trituberculatus innate immunity were analyzed. first, the mrna expression of ptlgbp in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (pamps) stimulations were examined using ...	2014	25194733
identification of a c-type lectin with antiviral and antibacterial activity from pacific white shrimp litopenaeus vannamei.	c-type lectins (ctls) play crucial roles in innate immune responses in invertebrates by recognizing and eliminating microinvaders. in this study, a ctl from pacific white shrimp litopenaeus vannamei (lvctl3) was identified. lvctl3 contains a single c-type lectin-like domain (ctld), which shows similarities to those of other shrimp ctls and has a mutated 'epd' motif in ca(2+)-binding site 2. lvctl3 mrna can be detected in all tested tissues and expression of lvctl3 in gills was up-regulated after ...	2014	24792214
quorum-quenching activity of the ahl-lactonase from bacillus licheniformis dahb1 inhibits vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality.	vibrio parahaemolyticus is a significant cause of gastroenteritis resulting from the consumption of undercooked sea foods and often cause significant infections in shrimp aquaculture. vibrio virulence is associated with biofilm formation and is regulated by n-acylated homoserine lactone (ahl)-mediated quorum sensing. in an attempt to reduce vibrio colonisation of shrimps and mortality, we screened native intestinal bacilli from indian white shrimps (fenneropenaeus indicus) for an isolate which s ...	2014	25060960
modelling and mutational analysis of aspergillus nidulans urea, a member of the subfamily of urea/h⁺ transporters in fungi and plants.	we present the first account of the structure-function relationships of a protein of the subfamily of urea/h(+) membrane transporters of fungi and plants, using aspergillus nidulans urea as a study model. based on the crystal structures of the vibrio parahaemolyticus sodium/galactose symporter (vsglt) and of the nucleobase-cation-symport-1 benzylhydantoin transporter from microbacterium liquefaciens (mhp1), we constructed a three-dimensional model of urea which, combined with site-directed and c ...	2014	24966243
microbiological quality of seasoned roasted laver and potential hazard control in a real processing line.	microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. laver or supplements were collected from six manufacturers (a to f) to assess potential microbiological hazards and the critical control points in commercial processing lines. aerobic plate counts (apc), coliform counts, bacillus cereus, staphylococcus aureus, and vibrio parahaemolyticus were enumerated, and the presence of b. cereus, listeria monocytogenes, salmonella, s. aureus, and v ...	2014	25474052
enhancement of the immune response and protection against vibrio parahaemolyticus by indigenous probiotic bacillus strains in mud crab (scylla paramamosain).	in a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. in this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. a total of 135 bacterial strains were isolated from three populations of mud crabs. the strains were screened for antagonistic activity against vibrio parahaemolyticus using an agar spot ass ...	2014	25193866
a multiplex pcr assay for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in korean ready-to-eat food.	a multiplex polymerase chain reaction (pcr) assay was developed for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in various korean ready-to-eat foods. the six specific primer pairs for multiplex pcr were selected based on the o157 antigen (rfbe) gene of e. coli o157:h7, the dna gyrase subunit b (gyrb) gene of b. cereus, the toxin regulatory protein (toxr) gene of v. parahaemolyticu ...	2014	24796416
novel myeloid differentiation factor 88, esmyd88, exhibits estube-binding activity in chinese mitten crab eriocheir sinensis.	myeloid differentiation factor 88 (myd88) is a universal and essential adapter protein that participates in the activation of the toll-like receptor/interleukin-1 receptor-mediated signaling pathway. in the present study, a new myd88 gene (named esmyd88) was identified in the chinese mitten crab eriocheir sinensis. the cdna of esmyd88 was 2210 bp long with a 1416 bp open reading frame that encoded a protein with 472 amino acids. predicted esmyd88 protein had a death domain at the n-terminal and ...	2014	25150191
cloning and characterization of two different l-type lectin genes from the chinese mitten crab eriocheir sinensis.	l-type lectins contain a leguminous lectin domain and bind to high-mannose type oligosaccharides. in the secretory pathway, l-type lectins play crucial functions in the trafficking, sorting, and targeting of maturing glycoproteins. this study identified two novel l-type lectins, designated as esergic-53 and esvip36, from the chinese mitten crab eriocheir sinensis. the complete nucleotide sequence of ergic-53 cdna was 1955 bp, containing a 1506 bp open reading frame (orf) encoding a putative prot ...	2014	24796868
liposome-encapsulated cinnamaldehyde enhances zebrafish (danio rerio) immunity and survival when challenged with vibrio vulnificus and streptococcus agalactiae.	cinnamaldehyde, which is extracted from cinnamon, is a natural compound with activity against bacteria and a modulatory immune function. however, the antibacterial activity and immunostimulation of cinnamaldehyde in fish has not been well investigated due to the compound's poor water solubility. thus, liposome-encapsulated cinnamaldehyde (lec) was used to evaluate the effects of cinnamaldehyde on in vitro antibacterial activity against aquatic pathogens and in vivo immunity and protection parame ...	2014	24632045
molecular cloning and characterization of the lipopolysaccharide and β-1,3-glucan binding protein from oriental river prawn, macrobrachium nipponense.	the lipopolysaccharide and β-1,3-glucan binding protein (lgbp), one of the pattern recognition proteins, plays an important role in the innate immune response of invertebrates. a 1,506 bp full-length cdna of a lgbp gene was cloned and characterized from the oriental river prawn macrobrachium nipponense (named as mnlgbp). analysis of the nucleotide sequence revealed that the cdna clone has an open reading frame of 1,119 bp, encoding a protein of 372 amino acids including a 21-aa signal peptide. t ...	2014	24584659
function of two novel single-crd containing c-type lectins in innate immunity from eriocheir sinensis.	c-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. in this study, two novel c-type lectin cdnas (esctl1 and esctl2) of eriocheir sinensis were cloned and characterized. esctl1 has 169 amino acids, whereas esctl2 has 164 amino acids. these two lectins contain one carbohydrate-recognition domain. phylogenetic analysis showed that esctl1 and esctl2 were not clustered with other reported lectins from crabs. esctl1 and esctl2 were expressed ...	2014	24561128
antimicrobial function of the gapdh-related antimicrobial peptide in the skin of skipjack tuna, katsuwonus pelamis.	a 3.4 kda of antimicrobial peptide was purified from an acidified skin extract of skipjack tuna, katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and c18 reversed-phase hplc. a comparison of the n-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high sequence homology with the yfgap (yellowfin tuna glyceraldehyde-3-phosphate dehydrogenase-related antimicrobial peptide); thus, this peptide was identified as the skip ...	2014	24412436
an ldla domain-containing c-type lectin is involved in the innate immunity of eriocheir sinensis.	c-type lectins (ctls) have crucial functions in recognizing and eliminating pathogens in innate immunity. this study identified a novel low-density lipoprotein receptor class a (ldla) domain-containing ctl, designated as esctldcp, from the chinese mitten crab eriocheir sinensis. the esctldcp cdna is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. esctldcp contains a signal peptide, an ldla, and a single c-type lectin-like domain. esctldcp was only expressed ...	2014	24140432
activities of alkyl hydroperoxide reductase subunits c1 and c2 of vibrio parahaemolyticus against different peroxides.	alkyl hydroperoxide reductase subunit c gene (ahpc) functions were characterized in vibrio parahaemolyticus, a commonly occurring marine food-borne enteropathogenic bacterium. two ahpc genes, ahpc1 (vpa1683) and ahpc2 (vp0580), encoded putative two-cysteine peroxiredoxins, which are highly similar to the homologous proteins of vibrio vulnificus. the responses of deletion mutants of ahpc genes to various peroxides were compared with and without gene complementation and at different incubation tem ...	2014	25239899
intracellular vibrio parahaemolyticus escapes the vacuole and establishes a replicative niche in the cytosol of epithelial cells.	vibrio parahaemolyticus is a globally disseminated gram-negative marine bacterium and the leading cause of seafood-borne acute gastroenteritis. pathogenic bacterial isolates encode two type iii secretion systems (t3ss), with the second system (t3ss2) considered the main virulence factor in mammalian hosts. for many decades, v. parahaemolyticus has been studied as an exclusively extracellular bacterium. however, the recent characterization of the t3ss2 effector protein vopc has suggested that thi ...	2014	25205094
transcriptome analysis of vibrio parahaemolyticus in type iii secretion system 1 inducing conditions.	vibrio parahaemolyticus is an emerging bacterial pathogen capable of causing inflammatory gastroenteritis, wound infections, and septicemia. as a food-borne illness, infection is most frequently associated with the consumption of raw or undercooked seafood, particularly shellfish. it is the primary cause of vibrio-associated food-borne illness in the united states and the leading cause of food-borne illness in japan. the larger of its two chromosomes harbors a set of genes encoding type iii sect ...	2014	24478989
copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host ampylation on self-assembled human protein microarrays.	ampylation (adenylylation) is a recently discovered mechanism employed by infectious bacteria to regulate host cell signaling. however, despite significant effort, only a few host targets have been identified, limiting our understanding of how these pathogens exploit this mechanism to control host cells. accordingly, we developed a novel nonradioactive ampylation screening platform using high-density cell-free protein microarrays displaying human proteins produced by human translational machiner ...	2014	25073739
vibrio type iii effector vpa1380 is related to the cysteine protease domain of large bacterial toxins.	vibrio parahaemolyticus is a gram-negative halophilic bacterium and one of the leading causes of food-borne gastroenteritis. its genome harbors two type iii secretion systems (t3ss1 and t3ss2), but only t3ss2 is required for enterotoxicity seen in animal models. effector proteins secreted from t3ss2 have been previously shown to promote colonization of the intestinal epithelium, invasion of host cells, and destruction of the epithelial monolayer. in this study, we identify vpa1380, a t3ss2 effec ...	2014	25099122
[polyneuropathy as an early symptom of neoplastic disease].		2014	4325143
[polyneuropathy as an early symptom of neoplastic disease].		2014	4325143
[experimental premisses to the introduction of i-131 labeled lipiodol "uf", in endosplenic administration, in the diagnosis and therapy of hepatic neoplasms].		2014	4291610
[experimental premisses to the introduction of i-131 labeled lipiodol "uf", in endosplenic administration, in the diagnosis and therapy of hepatic neoplasms].		2014	4291610
[serum phosphatases in gynecologic surgical cases].		2014	4320434
ankylosing spondylitis and primary hyperparathyroidism.		2014	4269196
prolonged extradural anaesthesia with bupivacaine at lumbago and sciatica.		2014	4259333
relative frequency of ovarian neoplasms in children and adolescents.		2014	4342408
a method for enhancing visualization of precipitin lines in agar analysis.		2014	4168942
the role of radiotherapy in the palliation of advanced bronchogenic carcinoma.		2014	4165897
[the kirghizia health service and prospects of its development].		2014	4155583
[ultrastructural changes of myocardial capillaries during extracorporeal circulation with anoxic arrest or with intermittent coronary perfusion (author's transl)].		2014	4435323