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[adhesive properties of microorganisms colonizing rheumatoid arthritis and systemic lupus erythematosus patients].evaluation of adhesive properties of microorganisms colonizing rheumatoid arthritis and systemic lupus erythematosus patients.201121598617
[beta-lactamases of clinical isolates of enterobacteriaceae family].among 62 clinical strains of enterobacteria, isolated from intestinal tract, representatives of genus escherichia made--67.4%, klebsiella--4.84%, citrobacter--4.84%, enterobacter--8.06%, proteus--6.45%, morganella of 8.06%. preparations of fluorquinolone group--gatifloxacin and moxifloxacin, the number of steady strains being from 0.0% to 33.3%, were the most effective in respect of enterobacteria the investigated strains of enterobacteria showed considerable resistance to beta-lactam antibiotic ...201121598655
postfragmentation density function for bacterial aggregates in laminar flow.the postfragmentation probability density of daughter flocs is one of the least well-understood aspects of modeling flocculation. we use three-dimensional positional data of klebsiella pneumoniae bacterial flocs in suspension and the knowledge of hydrodynamic properties of a laminar flow field to construct a probability density function of floc volumes after a fragmentation event. we provide computational results which predict that the primary fragmentation mechanism for large flocs is erosion. ...201121599205
cross-linking of dimeric cits and glts transport proteins.abstract cits of klebsiella pneumoniae and glts of escherichia coli are na(+)-dependent secondary transporters from different families that are believed to share the same fold and quaternary structure. a 10 kda protein tag (biotin acceptor domain [bad]) was fused to the n-terminus of both proteins (cits-bad1 and glts-bad1, respectively) and inserted in the central cytoplasmic loop that connects the two halves of the proteins (cits-bad260 and glts-bad206). both cits constructs and glts-bad206 wer ...201121599460
in vitro antimicrobial activity of ten medicinal plants against clinical isolates of oral cancer cases.abstract: background: suppression of immune system in treated cancer patients may lead to secondary infections that obviate the need of antibiotics. in the present study, an attempt was made to understand the occurrence of secondary infections in immuno-suppressed patients along with herbal control of these infections with the following objectives to: (a) isolate the microbial species from the treated oral cancer patients along with the estimation of absolute neutrophile counts of patients. (b) ...201121599889
comparisons of resistance of cf and non-cf pathogens to hydrogen peroxide and hypochlorous acid oxidants in vitro.abstract: background: cystic fibrosis (cf) lung disease has a unique profile of pathogens predominated by pseudomonas aeruginosa (psa) and staphylococcus aureus (sa). these microorganisms must overcome host immune defense to colonize the cf lungs. polymorphonuclear neutrophils are a major component of the host defense against bacterial infection. a crucial microbicidal mechanism is the production of oxidants including hydrogen peroxide (h2o2) and hypochlorous acid (hocl) by neutrophils to achiev ...201121599970
[the clinical efficacy and safety of intravenous cefmetazole in treatment of 1522 patients with aspiration pneumonia.]objective: to evaluate the efficacy and safety of intravenous cefmetazole in the treatment of patients with aspiratory pneumonia. methods: a multicenter, prospective and open-labeled trial was conducted. a total of 1522 patients were enrolled at the beginning, and only 1324 were evaluabled at the endpoint. the duration of treatment was 7 - 14 days. during the treatment and follow-up periods, we recorded any unexpected symptoms and abnormal laboratory tests. at last, we evaluated its efficacy and ...201121600147
effect of culture conditions on producing and uptake hydrogen flux of biohydrogen fermentation by metabolic flux analysis method.in this work, metabolic flux analysis (mfa) method was used to estimate the effects of the culture conditions on both the producing and uptake hydrogen flux inside the cell of klebsiella pneumoniae ecu-15. the results indicated that higher temperature could reduce the amount of the uptake hydrogen and enhance the hydrogen production from the nadh pathway. moreover, both the producing hydrogen flux from formate and the uptake hydrogen flux were attained to the maximum at ph 7.0-7.5. the producing ...201121602042
broad spectrum biofilm inhibition by kingella kingae exopolysaccharide.cell-free extracts prepared from kingella kingae colony biofilms were found to inhibit biofilm formation by aggregatibacter actinomycetemcomitans, klebsiella pneumoniae, staphylococcus aureus, s. epidermidis, candida albicans and k. kingae. the extracts evidently inhibited biofilm formation by modifying the physicochemical properties of the cell surface, the biofilm matrix, and the substrate. chemical and biochemical analyses indicated that the biofilm inhibition activity in the k. kingae extrac ...201121602333
simultaneous detection of methicillin-resistant staphylococcus aureus, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa by multiplex pcr.a pcr-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. six pairs of selected primers targeting fema (132 bp) and meca (310 bp) of methicillin-resistant staphylococcus aureus, glta (722 bp) of acinetobacter baumannii, phoa (903 bp) of escherichia coli, mdh (364 bp) of klebsiella pneumoniae and oprl (504 bp) of pseudomonas aeruginosa were used in this study. the conditions were optimized for the multiplex pcr to ensu ...201121602765
surgical approach to extensive hidradenitis suppurativa.background hidradenitis suppurativa (hs) is a chronic suppurative disease of skin with high recurrence. objective to determine factors affecting complications and recurrence of hs in patients who underwent surgery. materials and methods we operated on 15 patients with hs at 36 sites between 1999 and 2009. the affected areas were classified as groin, axilla, buttocks, nuchae, perianal, and perineal. all patients were treated using wide surgical excision under general anesthesia. the methods of re ...201121605246
bacteriological differences between patients with acute exacerbation of copd and community-acquired pneumonia.objective: to study the differences in pathogen distribution and antimicrobial drug susceptibility between patients with acute exacerbation chronic obstructive pulmonary disease (copd exacerbation) and community-acquired pneumonia (cap), thereby providing guidance for the antibiotic treatment of such diseases. methods: a retrospective analysis of the medical records from our patients with copd exacerbation and cap from january 2007 to december 2008 was conducted, where 586 copd exacerbation pati ...201121605476
sources other than unused sawdust can introduce klebsiella pneumoniae into dairy herds.a longitudinal study was carried out to detect intramammary infections caused by klebsiella pneumoniae and to identify potential sources of this bacterial species in the environment of the cows. the study was performed in 6 well-managed belgian dairy herds from may 2008 to may 2009. monthly (n=13), unused and used sawdust bedding samples as well as individual quarter milk and feces samples were collected from 10 randomly selected cohort cows in each herd. cases of clinical mastitis of all lactat ...201121605753
high prevalence of ctx-m-15-producing klebsiella pneumoniae isolates in asian countries: diverse clones and clonal dissemination.the characteristics of 218 klebsiella pneumoniae isolates from patients with hospital-acquired pneumonia in nine asian countries were investigated. in total, 92 isolates (42.2%) produced extended-spectrum β-lactamases (esbls), amongst which 67 (72.8%) possessed ctx-m esbl genes; ctx-m-15 was the major esbl (55 isolates; 59.8%). multilocus sequence typing (mlst) and plasmid replicon typing were performed to investigate the genetic backgrounds of the 55 ctx-m-15-producing k. pneumoniae isolates. t ...201121605960
[validity of using imipenem as a representative carbapenems and levofloxacin as a representative fluoroquinolones in antibiotics susceptibility test for enterobacteriaceae].in antimicrobial susceptibility test for enterobacteriaceae, the efficacies of carpapenems are predicted by the minimum inhibitory concentration (mic) of imipenem, and that of fluoroquinolones are predicted by the mic of levofloxacin. to assess its judgement, we compared the mics of imipenem, meropenem, panipenem, and doripenem for carbapenems, and ciprofloxacin, levofloxacin, tosufloxacin and pazufloxacin for fluoroquinolones of clinically isolated enterobacteria, escherichia coli, klebsiella p ...201121612040
synergistic effects and physiological responses of selected bacterial isolates from animal feed to four natural antimicrobials and two antibiotics.abstract in this study, 20 samples from three different sources of animal feed were investigated and six bacterial isolates were identified. the susceptibility of four natural antimicrobials, namely, eugenol, cinnamaldehyde, thymol, and carvacrol, against six of these isolates was determined. carvacrol and eugenol showed better inhibitory effects with larger zones of inhibition. the minimal inhibitory concentration for a range of antibiotics on the susceptibility of two isolates (namely, sphingo ...201121612425
prevalence and genetic characteristics of tem, shv, and ctx-m in clinical klebsiella pneumoniae isolates from saudi arabia.the prevalence and genetic basis of extended-spectrum beta-lactamases (esbls) in klebsiella pneumoniae remains unclear in saudi arabia. therefore, this study was devoted to determine the prevalence and characterize esbl-producing k. pneumoniae in al-qassim area, saudi arabia. a total of 430 isolates of k. pneumoniae isolated from clinical samples were collected over 6 months from january to june 2008. these isolates were screened for the presence of esbls by double-disk synergy test and re-evalu ...201121612509
emergence of extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae in danish hospitals; this is in part explained by spread of two ctx-m-15 clones with multilocus sequence types 15 and 16 in zealand. 201121612893
natural resistance of rose petals to microbial attack.petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ...201121614712
essential oil composition and antibacteral activity of vismia baccifera fruits collected from mérida, venezuela.the essential oil from the fruits of vismia baccifera triana & planch. (gutttiferae), collected in june 2009, was analyzed by gc/ms. a yield of 0.6% oil was obtained by hydrodistillation. twenty-seven components were identified by comparison of their mass spectra with those in the wiley gc-ms library data base. the major components were trans-cadin-l1,4-diene (36.6%), cis-cadin-1,4-diene (18.8%) and beta-caryophyllene (11.9%). the essential oil showed a broad spectrum of antibacterial activity a ...201121615035
antimicrobial activity of protease inhibitor from leaves of coccinia grandis (l.) voigt.antimicrobial activity of protease inhibitor isolated from coccinia grandis (l.) voigt. has been reported. a 14.3 kda protease inhibitor (pi) was isolated and purified to homogeneity by ammonium sulfate precipitation (20-85% saturation), sephadex g-75, deae sepharose column and trypsin-sepharose affinity chromatography from the leaves of c. grandis. the purity was checked by reverse phase high performance liquid chromatography. pi exhibited marked growth inhibitory effects on colon cell lines in ...201121615062
antiphospholipid syndrome in a neonate.antiphospholipid syndrome presenting in the neonatal period is very rare. although antiphospholipid antibodies from mothers with antiphospholipid syndrome can cross the placenta and put their neonates at risk, the occurrence of thrombotic complications in these neonates is uncommon. we present a 10-day-old neonate who developed klebsiella sepsis with arterial gangrene of the left lower limb. investigations revealed thrombosis of the left femoral artery with both the mother and the neonate positi ...201121615477
characterization of the structure and function of klebsiella pneumoniae allantoin racemase.the oxidative catabolism of uric acid produces 5-hydroxyisourate (hiu), which is further degraded to (s)-allantoin by two enzymes, hiu hydrolase and 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase. the intermediates of the latter two reactions, hiu and 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline, are unstable in solution and decay nonstereospecifically to allantoin. in addition, nonenzymatic racemization of allantoin has been shown to occur at physiological ph. since the further br ...201121616082
bio-generated metal binding polysaccharides as catalysts for synthetic applications and organic pollutant transformations.iron and palladium binding an exopolysaccharide (eps) were obtained and purified from cultures of bacterial cells of klebsiella oxytoca bas-10. the strain bas-10 was able to grow under anaerobic conditions with fe(iii)-citrate as energy and carbon source, producing fe(iii)-eps that was extracted and used as catalyst in the oxidation reaction of phenol with h(2)o(2). the same bacterial strain was cultivated anaerobically with na-citrate and pd(2)(no)(3) was added during the exponential growth to ...201121616180
prognostic factors and outcomes in endogenous klebsiella endophthalmitis. 201121616217
the newly discovered clpk protein strongly promotes survival of k. pneumoniae biofilm subjected to heat shock.not applicable.201121617023
etiology of septicemia in children with acute leukemia: 9-year experience from a children's hospital in china.bloodstream infection is an important cause of death among leukemia patients, and the etiologic agent surveillance is important for the prophylaxis and treatment. this study aims to identify the common bloodstream isolates in hospitalized leukemia patients with septicemia in our hospital, to choose the ideal combination of antimicrobial agents for infection prophylaxis and to clarify the appropriate time for antibiotic prophylaxis. to know this information, a retrospective analysis was conducted ...201121617561
potentiation of antimicrobial activity of ciprofloxacin by pelargonium graveolens essential oil against selected uropathogens.the recent approach of using herbs and antibiotics in combination constitutes a strategy to overcome the problems of resistance and side effects associated with conventional antibiotics. in the present study, the antimicrobial effect of pelargonium graveolens l' hér essential oil in combination with ciprofloxacin was evaluated on uropathogens, namely, klebsiella pneumoniae kt2, proteus mirabilis prt3 and staphylococcus aureus st2. minimum inhibitory concentrations of p. graveolens essential oil ...201121618302
comparative activity of tigecycline and tetracycline on gram-negative and gram-positive bacteria revealed by a multicentre study in four north european countries.abstract background: this study involves a multicentre surveillance of tigecycline and tetracycline activity against gram-negative and gram-positive bacteria from primary care centres (pccs), general hospital wards (ghws) and intensive care units (icus) in denmark (n = 9), finland (n = 10), norway (n = 7) and sweden (n = 19). methods: the hospitals were each asked to test 30 consecutive gram-positive and 30 gram-negative clinical isolates. supportive information accompanying each isolate include ...201121619494
antimicrobial resistance status and prevalence rates of extended spectrum beta-lactamase (esbl) producers isolated from a mixed human population.owing to the increasing epidemiological and therapeutic challenges associated with infections due to esbl producers, esbl prevalence rate among some bacteria isolates from healthy and non-healthy human population in a metropolitan nigerian setting was evaluated. a total of one hundred and forty-five (145) bacteria strains were isolated from a total of four hundred and sixty (460) samples collected from urine, wound, throat and anal swabs of 220 healthy volunteers in the community and from 240 pa ...201121619555
airway pathogens during the first year after lung transplantation: a single-center experience.after lung transplantation, a high level of immunosuppression is needed to prevent rejection. this demand renders recipients more sensitive to infections. as pulmonary infections are a major clinical problem during the first postoperative year after lung transplantation, preventive treatment and regular surveillance examinations are needed for immediate, adequate therapy. we describe the airway pathogens registered during the first posttransplantation year among our 12 lung transplant recipients ...201121620113
perfusion fluid contamination in relation to recipient survival and acute cellular rejection in orthotopic liver transplantation: retrospective analysis.a perfusion fluid used in the preservation of a grafted liver represents a medium suitable for microorganism growth. this study investigated the prevalence of perfusion fluid contamination, acute cellular rejection (acr) episodes, and patient survival rate.201121620118
complete (1)h and (13)c nmr chemical shift assignments of mono-, di-, and trisaccharides as basis for nmr chemical shift predictions of polysaccharides using the computer program casper.the computer program casper uses (1)h and (13)c nmr chemical shift data of mono- to trisaccharides for the prediction of chemical shifts of oligo- and polysaccharides. in order to improve the quality of these predictions the (1)h and (13)c, as well as (31)p when applicable, nmr chemical shifts of 30 mono-, di-, and trisaccharides were assigned. the reducing sugars gave two distinct sets of nmr resonances due to the α- and β-anomeric forms. in total 35 (1)h and (13)c nmr chemical shift data sets ...201121621752
profile of bacterial pneumonia during hajj.background & objectives : the congregation of a large number of people during hajj seasons from different parts of the world in overcrowded conditions within a confined area for a long period of time presents many public health challenges and health risks. one of the main health problems of the crowding is ease transmission of pneumonia by air droplets. this study was aimed to determine the most common causes of bacterial pneumonia during the 2005 hajj season and to relate the findings with clin ...201121623036
infection control practices need ingenuous processes beyond prescribed guidelines. 201121623118
[a case of acute phlegmonous gastritis causing gastroparesis and cured with medical treatment alone.]acute phlegmonous gastritis is an uncommon disease, often fatal condition characterized by suppurative bacterial infection of the gastric wall. it has a high mortality rate mainly because the diagnosis is usually made late. until recently, gastrectomy in combination with antibiotics was recommended. we had experienced a case of 66-year-old man presented with epigastric pain, nausea, vomiting, and hematemesis, followed by aspiration pneumonia. at upper gastrointestinal endoscopy, the gastric lume ...201121623140
[gastric wall abscess.]no abstract available.201121623144
phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth.a local herb, kacip fatimah, is famous amongst malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of labisia pumila benth. total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. leaves of var. pumi ...201121623314
clinical review of endogenous endophthalmitis in korea: a 14-year review of culture positive cases of two large hospitals.purpose: to identify the clinical features and outcomes of endogenous endophthalmitis in korea. materials and methods: we reviewed 18 patients with endogenous endophthalmitis at 2 korean hospitals, treated over a 14 year period between january 1993 and december 2006. results: the comorbidities observed in these cases were diabetes mellitus and liver cirrhosis. the most common pathogens, which were found in 7 patients each (38.9%), were klebsiella pneumonia and pseudomonas aeruginosa. all patient ...201121623606
air in the urinary system. 201120571353
elevated levels of anti-endotoxin antibodies in patients with bilateral idiopathic acute anterior uveitis.endotoxins have been proved to be responsible for acute anterior uveitis (aau) in animals in a well-established experimental model of endotoxin-induced uveitis (eiu). the purpose of our study was the detection of antibodies against endotoxins of selected enterobacteria in the serum of patients with idiopathic aau and searching for correlations between the levels of these antibodies and the presence of hla-b27 antigen as well as characteristic signs of eiu such as bilaterality and the absence of ...201120632997
1,3-propanediol production from crude glycerol from jatropha biodiesel process.the present report describes production of 1,3-propanediol by klebsiella pneumoniae atcc 15380 from crude glycerol from jatropha biodiesel process. optimization resulted in a yield of up to 56g/l of 1,3-propanediol. a conversion rate of 0.85mol 1,3-propanediol/mol of glycerol has been obtained. downstream processing to isolate 1,3-propanediol from the fermentation broth resulted in 99.7% pure product with a recovery of 34%. the pure 1,3-propanediol was polymerized with terephthalic acid successf ...201120601262
comparison of disk diffusion, etest and vitek2 for detection of carbapenemase-producing klebsiella pneumoniae with the eucast and clsi breakpoint systems.the aim of this study was to compare clsi and eucast mic and disk diffusion carbapenem breakpoints for the detection of carbapenemase-producing klebsiella pneumoniae. k. pneumoniae strains with known kpc (n = 31) or vim (n = 20) carbapenemases were characterized by disk diffusion (oxoid) and etest (biomérieux) vs. imipenem, meropenem and ertapenem, and with vitek2 (biomérieux, five different cards). extended-spectrum β-lactamase (esbl) testing was performed with vitek2 (biomérieux), esbl combina ...201120649801
host defence during klebsiella pneumonia relies on haematopoietic-expressed toll-like receptors 4 and 2.in this study, the relative roles of toll-like receptor (tlr)2 and tlr4 were investigated independently and together. moreover, we studied the role of haematopoietic compartment in anti-klebsiella host defence. we infected tlr2 and tlr4 single-, and tlr2×4 double knockout (ko) animals with different doses of klebsiella pneumoniae. in addition, bone marrow chimeric mice were created and infected. tlr4 played a more prominent role in antibacterial defence than tlr2, considering that only tlr4 ko m ...201120650991
the extent of amotosalen photodegradation during photochemical treatment of platelet components correlates with the level of pathogen inactivation.amotosalen plus ultraviolet a (uva) light inactivates a broad range of bacteria, viruses, protozoa, and leukocytes in platelet (plt) and plasma components. upon uva illumination a small fraction of amotosalen reacts with the nucleic acid of contaminating pathogens and residual white blood cells and the remaining fraction undergoes photodegradation into defined photoproducts. the levels of amotosalen and photoproducts can be accurately quantified.201120663111
a sensitive and specific phenotypic assay for detection of metallo-β-lactamases and kpc in klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin.enterobacteriaceae producing carbapenemases, such as kpc or metallo-β-lactamases (mbls), have emerged on several continents. phenotypic tests are urgently needed for their rapid and accurate detection. a novel carbapenemase detection test, comprising a meropenem disk, and meropenem disks supplemented with 730 μg of edta, 1000 μg of dipicolinic acid (dpa), 600 μg of aminophenylboronic acid (apba), or 750 μg of cloxacillin, was evaluated against klebsiella pneumoniae isolates with kpc (n = 34), vi ...201120597925
nosocomial infections and fever of unknown origin in pediatric hematology/oncology unit: a retrospective annual study.pediatric hematology/oncology patients are faced with an increased risk of nosocomial infections (nis) that vary in different populations and different institutions with considerable morbidity and mortality. this study was undertaken to assess the frequency and patterns of nis in 1564 pediatric patients and to determine the prevalence of causative organisms and their antimicrobial sensitivity.201120549409
successful treatment of endogenous klebsiella pneumoniae endophthalmitis: a case report.klebsiella pneumoniae endophthalmitis is rare and despite immediate management, visual outcome is generally poor. we report a case of endogenous k. pneumoniae endophthalmitis that was treated successfully.201120661624
genetic and functional diversity among the antagonistic potential fluorescent pseudomonads isolated from tea rhizosphere.twenty-five fluorescent pseudomonads from rhizospheric soil of six tea gardens in four district of upper assam, india were isolated and screened for antagonistic activity against fungal pathogens such as fusarium oxysporum f. sp. raphani (for), fusarium oxysporum f. sp. ciceri (foc), fusarium semitectum (fs), and rhizoctonia solani (rs); and bacterial pathogens-staphylococcus aureus (sa), escherichia coli (ec), and klebsiella pneumoniae (kp). most of the isolates exhibited strong antagonistic ac ...201120689953
toxicological effects of selective herbicides on plant growth promoting activities of phosphate solubilizing klebsiella sp. strain ps19.this study examines the effect of four herbicides, quizalafop-p-ethyl, clodinafop, metribuzin and glyphosate, on plant growth promoting activities like phosphate solubilization, siderophores, indole acetic acid, exo-polysaccharides, hydrogen cyanide and ammonia production by herbicide tolerant klebsiella sp. strain ps19. the strain was isolated from mustard rhizosphere. the selected herbicides were applied two to three times at the recommended rates. klebsiella sp. strain ps19 tolerated a concen ...201120721665
high prevalence of bla(ctx-m) extended spectrum beta-lactamase genes in klebsiella pneumoniae isolates from a tertiary care hospital: first report of bla(shv-12), bla(shv-31), bla(shv-38), and bla(ctx-m-15) in brazil.the aim of this study was to investigate the presence and prevalence of bla(tem), bla(shv), and bla(ctx-m) and bla(ges)-like genes, responsible for extended spectrum beta-lactamases (esbls) production in clinical isolates of klebsiella pneumoniae collected from a brazilian tertiary care hospital. sixty-five esbl producing k. pneumoniae isolates, collected between 2005 and 2007, were screened by polymerase chain reaction (pcr). identification of bla genes was achieved by sequencing. genotyping of ...201120795871
successful pelvic abscess drainage by cecal biopsies in crohn's disease. 201120719310
construction of stress-induced metabolic pathway from glucose to 1,3-propanediol in escherichia coli.1,3-propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive synthesis. the aim of this study was to engineer an escherichia coli strain that can produce 1,3-propanediol directly from glucose. we successfully constructed a stress-induced metabolic pathway from glucose to 1,3-propanediol in recombinant e. coli by the expression of gpd1 and gpp2 genes from saccharomyces cerevisiae and dha operon from klebsiella pneumoni ...201120803136
opportunistic bacterial infections in breeding colonies of the nsg mouse strain.spontaneous morbidity primarily affecting female breeders in 3 independent breeding colonies of nsg (nod.cg-prkdc(scid) i12rg(tm1wjl) /szj) mice prompted an investigation to uncover the cause of disease. necropsies were performed on 264 (157 female and 107 male) spontaneously sick, experimentally unmanipulated nsg mice. in sum, 42 mice (15.9%) had acute or chronic renal inflammatory lesions, of which 12 had concurrent histologic evidence of an ascending urinary tract infection. from 94 kidneys c ...201120817888
1,3-propandiol production by engineered hansenula polymorpha expressing dha genes from klebsiella pneumoniae.currently, 1,3-propanediol (1,3-pd) is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive chemical synthesis. a methylotrophic yeast hansenula polymorpha was engineered by expression of dhab1, dhab2, dhab3, dhab(ra1) and dhab(ra2) encoding glycerol dehydratase complex and dhat encoding 1,3-pd oxidoreductase from klebsiella pneumoniae under direction of promoter of glyceraldehyde-3 phosphate dehydrogenase (gapdh). the engineere ...201120820806
[fecal carriage of third generation cephalosporins resistant enterobacteteriaceae in asymptomatic young adults: evolution between 1999 and 2009].the aim of this work was to evaluate the fecal carriage of third generation cephalosporins resistant enterobacteriaceae in nonhospitalized asymptomatic young adults.201120828938
endotoxin does not alter the pharmacokinetics of micafungin, but it impairs biliary excretion of micafungin via multidrug resistance-associated protein 2 (abcc2/mrp2) in rats.micafungin, a newly developed echinocandin-type antifungal agent, is widely used for the treatment of deep-seated fungal infections including those of candida species and aspergillus species. in the present study, the possible alterations in the pharmacokinetics and biliary excretion of micafungin were investigated in endotoxemic rats induced by klebsiella pneumoniae endotoxin. endotoxin (2 mg/kg) was injected intraperitoneally 24 h before an intravenous injection of micafungin (1 mg/kg). no sig ...201120835743
[acquired bacterial meningitis in monastir region, tunisia (1999-2006): bacteriological aspects and susceptibility patterns].the aim of this study was to analyze the distribution of bacteria responsible for purulent meningitis and the pattern of resistance of common species in the university hospital of monastir (tunisia). all bacteriologically confirmed cases of bacterial meningitis were recorded between 1999 and 2006, and have been analyzed by classic bacterial methods advocate for meningitis. two hundred fifty three strains have been isolated. the most frequent species were streptococcus pneumoniae, haemophilus inf ...201120835902
synthesis and in vitro antimicrobial activity of novel n-(6-chlorobenzo[d]thiazol-2-yl) hydrazine carboxamide derivatives of benzothiazole class.in this study, a series of novel 1,2,4-triazolo-[3,4-b]-1,3,4-thiadiazole (6a-g) and 1,3,4-oxadiazole (7a-g, 8) were synthesized from n-(6-chlorobenzo[d]thiazol-2-yl) hydrazine carboxamide derivatives of benzothiazole class. antimicrobial properties of the title compound derivatives were investigated against one gram (+) bacteria (staphylococcus aureus), three gram (-) bacteria (escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae) and five fungi (candida albicans, aspergillus niger, ...201120807086
klebsiella pneumoniae subverts the activation of inflammatory responses in a nod1-dependent manner.klebsiella pneumoniae is an important cause of community-acquired and nosocomial pneumonia. subversion of inflammation is essential for pathogen survival during infection. evidence indicates that k. pneumoniae infections are characterized by lacking an early inflammatory response although the molecular bases are currently unknown. here we unveil a novel strategy employed by a pathogen to counteract the activation of inflammatory responses. k. pneumoniae attenuates pro-inflammatory mediators-indu ...201120846183
a novel mutation in the connexin 26 gene (gjb2) in a child with clinical and histological features of keratitis-ichthyosis-deafness (kid) syndrome.keratitis-ichthyosis-deafness (kid) syndrome is a rare congenital ectodermal disorder, caused by heterozygous missense mutation in gjb2, encoding the gap junction protein connexin 26. the commonest mutation is the p.asp50asn mutation, and only a few other mutations have been described to date.201120846357
[etiology and sensitivity of uropathogens identified in uncomplicated lower urinary tract infections in women (aresc study): implications on empiric therapy].to determine the etiology and susceptibility of uropathogens identified in women with uncomplicated lower urinary tract infections (uti).201120889171
risk factors for hospital-acquired urinary tract infection: a case-control study.the objective is to assess risk factors and microbiological aspects of hospital-acquired urinary tract infection (hauti) on six wards of a general regional hospital in serbia. a case-control study was nested within prospective cohort hautis study conducted from january 1 to december 31, 2007. three controls were identified for each patient with hauti, being chronologically the next three patients surveyed who did not develop hauti. the patients and controls were matched by sex and age (±5 years) ...201120848199
characterization of a defined cellulolytic and xylanolytic bacterial consortium for bioprocessing of cellulose and hemicelluloses.diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. in an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, p ...201120859703
clinical and molecular epidemiology of extended-spectrum β-lactamase-producing escherichia coli and klebsiella pneumoniae in a long-term study from japan.the detection rates of extended-spectrum β-lactamase (esbl)-producing bacteria in japan are very low (∼5%) compared with those obtained worldwide. further, the current trend of these bacteria in japan is not known, and few studies with longitudinal observations have been reported. to obtain epidemiologic data on esbl-producing bacteria, their genotypic features, and their antibiotic resistance patterns in japan, we analyzed bacterial isolates from hospitalized patients at our institution over th ...201120859753
impact of microbial tolerance in persistent secondary klebsiella pneumoniae peritonitis.microbial tolerance represents a diminished pro-inflammatory response following repeated stimulation by a host of pathogen-associated molecular patterns (pamps) of varying origins. toll-like receptors (tlrs) have been centrally implicated in the development of tolerance. the purpose of this study was to investigate the impact of tolerance in a previously described murine model. c57bl/6 mice were pretreated intraperitoneally with phosphate buffered saline (pbs), heat-killed klebsiella 2×10(8) cfu ...201120869262
triggering receptor (trem-1) expressed on myeloid cells predicts bacteremia better than clinical variables in community-acquired pneumonia.some clinical variables are associated with bacteremia in patients with community-acquired pneumonia (cap). the aim of this study was to analyse the accuracy of the soluble form of triggering receptor expressed on myeloid cells-1 (strem-1) to predict positive blood cultures in comparison with established clinical prognostic variables.201121114709
pharmacokinetics of polymyxin b in an infant with multidrug-resistant klebsiella pneumoniae bacteremia.we report our experience with a 9-month-old infant treated with intravenous polymyxin b for multidrug-resistant klebsiella pneumoniae bacteremia. serial blood samples were obtained at steady state and serum drug concentrations were determined using a validated liquid chromatography-mass spectrometry method. the elimination half-lives of polymyxin b1 and isoleucine-polymyxin b1 were found to be 3.1 and 4.7 hours, respectively.201121164382
deep neck abscesses: the singapore experience.this study aims to review our experience with deep neck abscesses, identify key trends, and improve the management of this condition. this is a retrospective chart review of patients diagnosed with deep neck abscesses in the department of ent (otorhinolaryngology) at tan tock seng hospital, singapore between 2004 and 2009. patient demographics, etiology, bacteriology, systemic disease, radiology, treatment, complications, duration of hospitalization, and outcomes were reviewed. 131 patients were ...201120857130
gas in the renal shadow of a plain abdominal x-ray: emphysematous pyelonephritis. 201121175990
new delhi metallo-β-lactamase in klebsiella pneumoniae and escherichia coli, canada.multidrug-resistant klebsiella pneumoniae and escherichia coli isolates harboring new delhi metallo-β-lactamase (ndm-1) were isolated from a patient who had returned to canada from india. the ndm-1 gene was found on closely related incompatibility group a/c type plasmids. the occurrence of ndm-1 in north america is a major public health concern.201121192866
emergence of new delhi metallo-β-lactamase, austria. 201121192874
antimicrobial activity of erythromycin and clarithromycin against clinical isolates of escherichia coli, staphylococcus aureus, klebsiella and proteus by disc diffusion method.fifty clinical isolates comprising of escherichia coli, staphylococcus aureus, klebsiella and proteus were collected from different local pathological laboratories and their resistant pattern against two well known macrolides; erythromycin and clarithromycin were studied using disc diffusion method. klebsiella (41.67% against erythromycin and 58.34% against clarithromycin) and proteus (66.67% against erythromycin and clarithromycin) species were found to be more resistant against the studied mac ...201121190914
rhinoscleroma: an updated experience through the last 10 years.rhinoscleroma is a chronic, specific, granuloma of the nose and other parts of the respiratory system. the disease is endemic in egypt and many other countries. the causative organism is klebsiella rhinoscleromatis bacillus, proved by fulfilling koch's postulates. the mode of infection is not known and its worldwide irregular geographical distribution is not understood. lines of treatment are unsatisfactory and a tendency for recurrence is the rule.201121198342
purification and properties of antimicrobial peptides from skin secretions of the eritrea clawed frog xenopus clivii (pipidae).five peptides with antimicrobial activity were isolated from norepinephrine-stimulated skin secretions of the tetraploid frog xenopus clivii peracca, 1898 (pipidae). characterization of the peptides demonstrated that they are structurally similar to magainins (2 peptides), caerulein-precursor fragments, cpf (2 peptides), and xenopsin-precursor fragments, xpf (1 peptide) that have been previously isolated from other species of the genus xenopus. the magainins and the xpf peptide were active only ...201121199684
detection of klebsiella pneumoniae carbapenemase (kpc) production in non-klebsiella pneumoniae enterobacteriaceae isolates by use of the phoenix, vitek 2, and disk diffusion methods.in this study, we tested the abilities of the vitek 2, bd phoenix, and kirby bauer disk diffusion tests to detect carbapenemase production in a collection of 14 klebsiella pneumoniae carbapenemase (kpc)-producing non-klebsiella pneumoniae isolates. in addition, we evaluated 13 kpc-positive k. pneumoniae isolates by using each of these methods and applied both 2009 and 2010 clsi carbapenem interpretive guidelines.201121209164
inhibition of klebsiella pneumoniae dnab helicase by the flavonol galangin.klebsiella pneumoniae is a ubiquitous opportunistic pathogen that colonizes at the mucosal surfaces in humans and causes severe diseases. many clinical strains of k. pneumoniae are highly resistant to antibiotics. here, we used fluorescence quenching to show that the flavonols galangin, myricetin, quercetin, and kaempferol, bearing different numbers of hydroxyl substituent on the aromatic rings, may inhibit dntp binding of the primary replicative dnab helicase of k. pneumoniae (kpdnab), an essen ...201121210194
evaluation of three phenotypic identification systems for clinical isolates of raoultella ornithinolytica.raoultella spp. have recently been separated from the genus klebsiella based on their molecular characteristics. it was discovered that raoultella ornithinolytica can be misidentified as klebsiella oxytoca by commonly used phenotypic identification systems. therefore, this study evaluated the ability of three phenotypic systems to identify r. ornithinolytica compared with the genotypic methods sequence-specific primer pcr (ssp-pcr), 16s rrna gene sequence analysis using the microseq 500 system16 ...201121212145
volatile compounds and antimicrobial and antioxidant activities of the essential oils of the needles of pinus densiflora and pinus thunbergii.to investigate the volatile compounds and the antibacterial and antioxidant effects of the essential oils of pinus densiflora needles (epdn) and pinus thunbergii needles (eptn), the volatile compounds of steam-distilled essential oils were analysed by gas chromatography-mass spectrometry. antibacterial activities were analysed by performing disc-agar diffusion assay and determining the minimum inhibitory concentrations (mics) of the essential oils. antioxidant activities were analysed via radica ...201121213230
kpc-2 carbapenemase-producing klebsiella pneumoniae isolates from patients with cystic fibrosis.bacteria producing klebsiella pneumoniae carbapenemases (kpcs) are rapidly emerging as a cause of multidrug-resistant infections worldwide. kpcs enzyme are plasmid-borne and can accumulate and transfer resistance determinants to other classes of antibiotics.we report two cases of kpc-2 carbapenemase-producing klebsiella pneumoniae isolates from cystic fibrosis patients.201121220216
new delhi metallo-β-lactamase-1 in enterobacteriaceae: emerging resistance. 201121220461
occurrence of kpc-2-producing klebsiella pneumoniae strains in hospital wastewater. 201121216031
[effects of chinese herbal medicine dusuqing granule on toll-like receptor 4 signaling in multiple organ injury induced by bacterial pneumonia in aged rats].to study the protective mechanism of dusuqing granule, a compound chinese herbal medicine, on the senile multiple organ injury caused by bacterial pneumonia by observing the expression changes of molecules related to toll-like receptor 4 (tlr4) signaling.201121227038
the iκb family member bcl-3 coordinates the pulmonary defense against klebsiella pneumoniae infection.bcl-3 is an atypical member of the iκb family that has the potential to positively or negatively modulate nuclear nf-κb activity in a context-dependent manner. bcl-3's biologic impact is complex and includes roles in tumorigenesis and diverse immune responses, including innate immunity. bcl-3 may mediate lps tolerance, suppressing cytokine production, but it also seems to contribute to defense against select systemic bacterial challenges. however, the potential role of bcl-3 in organ-specific ho ...201121228348
improving turnaround time for identifying extended-spectrum β-lactamase-producing escherichia coli and klebsiella pneumoniae: experience with the bd phoenix automated system. 201121233294
combined bone and soft-tissue augmentation surgery in temporo-orbital contour reconstruction.temporal hollowing due to temporal muscle atrophy after standard skull base surgery is common. various techniques have been previously described to correct the disfiguring defect. most often reconstruction is performed using freehand molded polymethylmethacrylate cement. this method and material are insufficient in terms of aesthetic results and implant characteristics. we herein propose reconstruction of such defects with a polyetheretherketone (peek)-based patient-specific implant (psi) includ ...201121233740
carijodienone from the octocoral carijoa multiflora. a spiropregnane-based steroid.two new steroids (1 and 2) and the known pregna-1,4,20-trien-3-one (3) have been isolated from the pacific octocoral carijoa multiflora. compound 1 possesses a novel spiropregnane-based steroidal skeleton. the photochemical transformation of 3 into 1 allowed the assignment of the absolute configuration at c-10 of 1. the antibacterial activities of compounds 1 and 3 were evaluated against a panel of bacterial strains.201121235217
first report of plasmid-mediated quinolone resistance qnra1 gene in klebsiella pneumoniae isolate of animal origin.one qnra1-producing klebsiella pneumoniae isolate gdka1 from chicken was detected. the qnra1 gene on plasmid pgdka1 was located in a genetic environment similar to that in in36 on plasmid phsh1 and could be cotransferred to escherichia coli j53 az(r) with other resistances by a conjugation experiment. upstream of the qnra1 gene, there was a class i integron with the dfra27 and aada2 cassettes. similar genetic environments of qnra1 in enterobacteriaceae isolates from both human and animal origin ...201121235404
activity of tigecycline alone and in combination with colistin and meropenem against klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae strains by time-kill assay.antibiotic combinations including tigecycline have not been studied against klebsiella pneumoniae carbapenemase (kpc)-producing pathogens. tigecycline alone and combined with colistin and meropenem was tested against eight genetically unrelated kpc-producing clinical strains of enterobacteriaceae (four k. pneumoniae, two escherichia coli, one enterobacter cloacae and one serratia marcescens) by time-kill assay. tigecycline displayed a concentration-independent bacteriostatic activity in seven st ...201121236643
on the detection of plasmidic ampc in enterobacteriaceae other than escherichia spp. and klebsiella spp. 201121237619
contribution of the klebsiella pneumoniae capsule to bacterial aggregate and biofilm microstructures.we studied the interaction between capsule production and hydrodynamic growth conditions on the internal and macroscopic structure of biofilms and spontaneously formed aggregates of klebsiella pneumoniae. wild-type and capsule-deficient strains were studied as biofilms and under strong and mild hydrodynamic conditions. internal organization of multicellular structures was determined with a novel image-processing algorithm for feature extraction from high-resolution confocal microscopy. measures ...201121239544
structural and mechanical properties of klebsiella pneumoniae type 3 fimbriae.this study investigated the structural and mechanical properties of klebsiella pneumoniae type 3 fimbriae, which constitute a known virulence factor for the bacterium. transmission electron microscopy and optical tweezers were used to understand the ability of the bacterium to survive flushes. an individual k. pneumoniae type 3 fimbria exhibited a helix-like structure with a pitch of 4.1 nm and a three-phase force-extension curve. the fimbria was first nonlinearly stretched with increasing force ...201121239584
nationwide survey of antibacterial activity against clinical isolates from urinary tract infections in japan (2008).in this study, the causative bacteria and their sensitivity to various antimicrobial agents as well as risk factors for quinolone-resistant escherichia coli were investigated in patients with acute uncomplicated cystitis or complicated cystitis by isolation and culture of bacteria from urine samples. in total, 1312 strains were isolated from 1009 patients with acute uncomplicated cystitis, including e. coli (63.3%), enterococcus faecalis (12.8%) and streptococcus agalactiae (4.6%). in addition, ...201121242062
overexpression of sicam-1 in the alveolar epithelial space results in an exaggerated inflammatory response and early death in gram negative pneumonia.a sizeable body of data demonstrates that membrane icam-1 (micam-1) plays a significant role in host defense in a site-specific fashion. on the pulmonary vascular endothelium, micam-1 is necessary for normal leukocyte recruitment during acute inflammation. on alveolar epithelial cells (aecs), we have shown previously that the presence of normal micam-1 is essential for optimal alveolar macrophage (am) function. we have also shown that icam-1 is present in the alveolar space as a soluble protein ...201121247482
pulmonary nocardiosis caused by nocardia exalbida complicating pneumocystis pneumonia in an hiv-infected patient.a 47-year-old man with optimally controlled type-2 diabetes mellitus and chronic hepatitis b was admitted to a local hospital because of a 1-week history of cough and high-grade fever. he was diagnosed with pneumocystis pneumonia (pcp) and klebsiella pneumonia from a chest radiograph and sputum. simultaneously, he was found to have hiv infection with a cd4 count of 76/μl. despite alteration of treatment secondary to the development of allergic reaction to trimethoprim-sulfamethoxazole (tmp-smx), ...201121249414
enterobacterial microflora in infancy - a case study with enhanced enrichment.to validate pmeu (portable microbe enrichment unit) method for monitoring the composition and development of infantile intestinal enterobacterial microflora.201121249478
[antibiotic resistance of gram-negative bacillis isolated from children with bronchopneumonia].to study the distribution and antibiotic resistance of gram-negative pathogens isolated from children with bronchopneumonia.201121251380
tigecycline activity tested against multidrug-resistant enterobacteriaceae and acinetobacter spp. isolated in us medical centers (2005-2009).we evaluated the activity of tigecycline against enterobacteriaceae (9563 isolates) and acinetobacter spp. (835) with various resistance phenotypes collected from 31 us medical centers in 2005-2009. the isolates were tested for susceptibility by the reference broth microdilution method against tigecycline and various comparators. among escherichia coli and klebsiella spp., 6.8% and 15.4% exhibited an extended-spectrum β-lactamase (esbl) phenotype, respectively; and 22.2% of enterobacter spp. str ...201121251571
emergence of kpc-producing klebsiella pneumoniae in texas. 201121251574
new genetic environments of aac(6')-ib-cr gene in a multiresistant klebsiella oxytoca strain causing an outbreak in a pediatric intensive care unit. 201121251575
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