Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| antimicrobial and antioxidative enrichment of oak (quercus robur) bark by rotation planar extraction using extrachrom. | the multifunctional extrachrom instrument was used in the extraction of antimicrobial and radical scavenging components from oak (quercus robur l.) bark. milled and sieved oak bark was extracted with 80% (v/v) methanol solution in water on the extrachrom instrument using step-gradient in the preparative separation. extracts were tested using agar diffusion method on staphylococcus aureus, enterobacter aerogenes and candida albicans. some extracts showed moderate bactericidal, fungicidal, bacteri ... | 2004 | 15109795 |
| nickel trafficking: insights into the fold and function of uree, a urease metallochaperone. | uree is a metallo-chaperone assisting the incorporation of two adjacent ni(2+) ions in the active site of urease. this study describes an attempt to distill general information on this protein using a computational post-genomic approach for the understanding of the structural details of the molecular function of uree in nickel trafficking. the two crystal structures recently determined for uree from bacillus pasteurii (bpuree) and klebsiella aerogenes (kauree) were comparatively analyzed. this a ... | 2004 | 15134926 |
| evaluation of a new cefepime-clavulanate esbl etest to detect extended-spectrum beta-lactamases in an enterobacteriaceae strain collection. | in this study, we evaluated the performance of a new esbl etest configuration based on clavulanate synergy with cefepime compared with cefotaxime-clavulanate and ceftazidime-clavulanate esbl etest strips for the detection of extended-spectrum beta-lactamases (esbl) in an enterobacteriaceae strain collection, with special focus on enterobacter spp. | 2004 | 15150168 |
| omp35, a new enterobacter aerogenes porin involved in selective susceptibility to cephalosporins. | in enterobacter aerogenes, beta-lactam resistance often involves a decrease in outer membrane permeability induced by modifications of porin synthesis. in atcc 15038 strain, we observed a different pattern of porin production associated with a variable antibiotic susceptibility. we purified omp35, which is expressed under conditions of low osmolality and analyzed its pore-forming properties in artificial membranes. this porin was found to be an ompf-like protein with high conductance values. it ... | 2004 | 15155215 |
| use of pharmacodynamic end points in the evaluation of gatifloxacin for the treatment of acute maxillary sinusitis. | the relationship between drug exposure and the time course of antimicrobial effect at the primary infection site for acute maxillary sinusitis has not previously been explored. this single-center, open-label study quantified the time course of sinus sterilization, described gatifloxacin exposure at the infection site, and posed the hypothesis that the use of continuous and quantitative time-related end points may allow for better characterization of drug effect with fewer patients than tradition ... | 2004 | 15156435 |
| comparative in vitro and in vivo study of nine alcohol-based handrubs. | hygienic hand disinfection using alcohol-based handrubs (ahrs) is an alternative method to conventional handwashing in hospital wards. because a documented choice of such products would consider data from in-care evaluation, we designed a comparative study of 9 ahrs both in vitro and in vivo in actual care conditions. | 2004 | 15175613 |
| antiseptic impregnated endotracheal tubes for the prevention of bacterial colonization. | the effect of endotracheal tubes (etts) impregnated with chlorhexidine (chx) and silver carbonate (antiseptic etts) against staphylococcus aureus, methicillin-resistant s. aureus (mrsa), pseudomonas aeruginosa, acinetobacter baumannii, and enterobacter aerogenes [organisms associated with ventilator-associated pneumonia (vap)], was evaluated in a laboratory airway model. antiseptic etts and control etts (unimpregnated) were inserted in culture tubes half-filled with agar media (airway model) pre ... | 2004 | 15183249 |
| the outer membrane protein omp35 affects the reduction of fe(iii), nitrate, and fumarate by shewanella oneidensis mr-1. | shewanella oneidensis mr-1 uses several electron acceptors to support anaerobic respiration including insoluble species such as iron(iii) and manganese(iv) oxides, and soluble species such as nitrate, fumarate, dimethylsulfoxide and many others. mr-1 has complex branched electron transport chains that include components in the cytoplasmic membrane, periplasm, and outer membrane (om). previous studies have implicated a role for anaerobically upregulated om electron transport components in the use ... | 2004 | 15212692 |
| rama is an alternate activator of the multidrug resistance cascade in enterobacter aerogenes. | multidrug resistance (mdr) in enterobacter aerogenes can be mediated by induction of mara, which is triggered by certain antibiotics and phenolic compounds. in this study, we identified the gene encoding rama, a 113-amino-acid regulatory protein belonging to the arac-xyls transcriptional activator family, in the enterobacter aerogenes atcc 13048 type strain and in a clinical multiresistant isolate. overexpression of rama induced an mdr phenotype in drug-susceptible escherichia coli jm109 and e. ... | 2004 | 15215103 |
| evaluation of the uro-quick, a new rapid automated system, for the detection of well-characterized antibiotic-resistant bacteria. | the uro-quick system has been employed to detect antibiotic resistance in genotypically and/or phenotypically well-characterized bacterial species including those that might not be easily identified by routine procedure. in order to achieve full agreement between the antibiotic susceptibility results obtained by the reference method (nccls) and the uro-quick system, the optimal experimental conditions (inoculum size, time of incubation and antibiotic concentration) for each strain to be used by ... | 2004 | 15216942 |
| enterobacter aerogenes ompx, a cation-selective channel mar- and osmo-regulated. | the ompx gene of enterobacter aerogenes was cloned. its overexpression induced a decrease in the major porin omp36 production and consequently a beta-lactam resistance was noted. purified outer membrane protein x (ompx) was reconstituted into artificial membranes and formed ion channels with a conductance of 20 ps in 1 m nacl and a cationic selectivity. both mara expression and high osmolarity induced a noticeable increase of the ompx synthesis in the e. aerogenes atcc 13048 strain. in addition, ... | 2004 | 15225603 |
| evaluation of the hyplex bloodscreen multiplex pcr-enzyme-linked immunosorbent assay system for direct identification of gram-positive cocci and gram-negative bacilli from positive blood cultures. | we evaluated the hyplex bloodscreen pcr-enzyme-linked immunosorbent assay (elisa) system (bag, lich, germany), a new diagnostic test for the direct identification of gram-negative bacilli and gram-positive cocci from positive blood cultures, with 482 positive bactec 9240 blood culture bottles. the test involves amplification of the bacterial dna by multiplex pcr and subsequent hybridization of the pcr product to specific oligonucleotide probes in an elisa-based format. the available probes allow ... | 2004 | 15243074 |
| emerging from obscurity: biological, clinical, and diagnostic aspects of dientamoeba fragilis. | ever since its first description in 1918, dientamoeba fragilis has struggled to gain recognition as a significant pathogen. there is little justification for this neglect, however, since there exists a growing body of case reports from numerous countries around the world that have linked this protozoal parasite to clinical manifestations such as diarrhea, abdominal pain, flatulence, and anorexia. a number of studies have even incriminated d. fragilis as a cause of irritable bowel syndrome, aller ... | 2004 | 15258093 |
| frequency and diversity of class a extended-spectrum beta-lactamases in hospitals of the auvergne, france: a 2 year prospective study. | to evaluate the frequency and diversity of extended-spectrum beta-lactamases (esbls) produced by enterobacteriaceae and pseudomonas aeruginosa in one french region. | 2004 | 15282240 |
| cloning and expression of the vitreoscilla hemoglobin gene in enterobacter aerogenes: effect on cell growth and oxygen uptake. | the hemoglobins found in unicellular organisms show a greater chemical reactivity, protect cells against oxidative stress and hence have been implicated in a wider variety of potential functions than those traditionally associated with animal and plant hemoglobins. there are well-documented studies showing that bacteria expressing vitreoscilla hemoglobin (vhb), the first prokaryotic hemoglobin characterized, have better growth and oxygen uptake rates than vhb counterparts. | 2004 | 15283330 |
| spermidine/spermine-n1-acetyltransferase-2 (ssat2) acetylates thialysine and is not involved in polyamine metabolism. | spermidine/spermine-n1-acetyltransferase (ssat1) is a short-lived polyamine catabolic enzyme inducible by polyamines and polyamine analogues. induction of ssat1 plays an important role in polyamine homoeostasis, since the n1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase. we have purified a recombinant human acetyltransferase (ssat2) that shares 45% identity and 61% homology with human ssat1, but is only distally related to other known members of the gnat (gcn5-rela ... | 2004 | 15283699 |
| recommended test panel for differentiation of klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical tests. | klebsiella pneumoniae and klebsiella oxytoca are the two most frequently encountered klebsiella species giving rise to infections in humans, but other klebsiella species can also be found in clinical specimens: klebsiella ozaenae, klebsiella rhinoscleromatis, klebsiella terrigena, klebsiella planticola, klebsiella ornithinolytica, and enterobacter aerogenes (klebsiella mobilis). however, many of these species are indistinguishable by the conventional methods employed routinely in the clinical mi ... | 2004 | 15297514 |
| molecular epidemiology of enterobacteriaceae isolates producing extended-spectrum beta-lactamases in a french hospital. | in 2002, 80 isolates of enterobacteriaceae producing extended-spectrum beta-lactamases (esbls) were collected from infected patients in our hospital. enterobacter aerogenes was the most common bacterium isolated from all specimens (36.5%). the esbls were predominantly (90%) tem derivatives (tem-24, tem-3). pulsed-field gel electrophoresis highlighted that e. aerogenes, klebsiella pneumoniae, and citrobacter koseri had a clonal propagation. | 2004 | 15297534 |
| enhanced production of acetoin and butanediol in recombinant enterobacter aerogenes carrying vitreoscilla hemoglobin gene. | microbial production of butanediol and acetoin has received increasing interest because of their diverse potential practical uses. although both products are fermentative in nature, their optimal production requires a low level of oxygen. in this study, the use of a recombinant oxygen uptake system on production of these metabolites was investigated. enterobacter aerogenes was transformed with a puc8-based plasmid carrying the gene (vgb) encoding vitreoscilla (bacterial)hemoglobin (vhb). the pre ... | 2004 | 15309606 |
| the acrab-tolc pump is involved in macrolide resistance but not in telithromycin efflux in enterobacter aerogenes and escherichia coli. | the role of the acrab-tolc pump in macrolide and ketolide susceptibility in escherichia coli and enterobacter aerogenes was studied. efflux pump inhibitor restored erythromycin, clarithromycin, and telithromycin susceptibilities to multidrug-resistant isolates. no modification of telithromycin accumulation was detected in e. aerogenes acrab or tolc derivatives compared to that in the parental strain. two independent efflux pumps, inhibited by phenylalanine arginine beta-naphthylamide, expel macr ... | 2004 | 15328143 |
| co-infection of the macronucleus of paramecium caudatum by free-living bacteria together with the infectious holospora obtusa. | infection experiments were performed incubating paramecium caudatum with non-infectious free-living bacteria or weakly infectious intracellular bacteria together with the infectious holospora obtusa. two of four non-infectious free-living bacteria (enterobacter aerogenes and klebsiella pneumoniae) were found to get into the nuclei when added to paramecium together with h. obtusa. the endonuclear bacterium nonospora macronucleata that is weakly infectious by itself increases its infectivity when ... | 2004 | 15352323 |
| dissemination of transferable ampc-type beta-lactamase (cmy-10) in a korean hospital. | to determine dissemination and genotype of ampc beta-lactamases and an extended-spectrum beta-lactamase among clinical isolates of enterobacteriaceae, we performed antibiotic susceptibility testing, pi determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (eric)-pcr, and dna sequencing. among the 51 clinical isolates collected from a university hospital in korea, six isolates were resistant to cephamycins. all six isolates produced a plasmid ... | 2004 | 15383166 |
| production of l-asparaginase in enterobacter aerogenes expressing vitreoscilla hemoglobin for efficient oxygen uptake. | this study is the first utilizing vitreoscilla hemoglobin in a heterologous bacterium, enterobacter aerogenes, to determine the effect of such a highly efficient oxygen-uptake system on the production of l-asparaginase, an enzyme that has attracted considerable attention due to its anti-tumor activity. here, we show that the vitreoscilla hemoglobin expressing strain has from 10-fold to more than two orders of magnitude lower l-asparaginase activity than the wild type or the control without the v ... | 2004 | 14593509 |
| structure and characterization of flavolipids, a novel class of biosurfactants produced by flavobacterium sp. strain mtn11. | herein we report the structure and selected properties of a new class of biosurfactants that we have named the flavolipids. the flavolipids exhibit a unique polar moiety that features citric acid and two cadaverine molecules. flavolipids were produced by a soil isolate, flavobacterium sp. strain mtn11 (accession number ay162137), during growth in mineral salts medium, with 2% glucose as the sole carbon and energy source. mtn11 produced a mixture of at least 37 flavolipids ranging from 584 to 686 ... | 2004 | 14711632 |
| growth of escherichia coli coexpressing phosphotriesterase and glycerophosphodiester phosphodiesterase, using paraoxon as the sole phosphorus source. | phosphotriesterases catalyze the hydrolytic detoxification of phosphotriester pesticides and chemical warfare nerve agents with various efficiencies. the directed evolution of phosphotriesterases to enhance the breakdown of poor substrates is desirable for the purposes of bioremediation. a limiting factor in the identification of phosphotriesterase mutants with increased activity is the ability to effectively screen large mutant libraries. to this end, we have investigated the possibility of cou ... | 2004 | 14711669 |
| selection during cefepime treatment of a new cephalosporinase variant with extended-spectrum resistance to cefepime in an enterobacter aerogenes clinical isolate. | enterobacter aerogenes resistant to cefepime (mic, 32 microg/ml) was isolated from a patient treated with cefepime for an infection caused by a strain of e. aerogenes overproducing its ampc beta-lactamase (mic of cefepime, 0.5 microg/ml). the ampc beta-lactamase of the resistant strain had an l-293-p amino acid substitution and a high k(cat)/k(m) ratio for cefepime. both of these modifications were necessary for resistance to cefepime. | 2004 | 14982805 |
| inhibitors of antibiotic efflux in resistant enterobacter aerogenes and klebsiella pneumoniae strains. | in enterobacter aerogenes and klebsiella pneumoniae, efflux provides efficient extrusion of antibiotics and contributes to the multidrug resistance phenotype. one of the alkoxyquinoline derivatives studied here, 2,8-dimethyl-4-(2'-pyrrolidinoethyl)-oxyquinoline, restores noticeable drug susceptibility to resistant clinical strains. analyses of energy-dependent chloramphenicol efflux indicate that this compound inhibits the efflux pump mechanism and improves the activity of structurally unrelated ... | 2004 | 14982806 |
| neonatal sepsis and meningitis in haiti. | the aim of the study was to determine the etiology of meningitis and sepsis in the newborn at the state university hospital of haiti and evaluate the susceptibility 'in vitro' of the pathogens to the antibiotics commonly used. this was a prospective case series study over a 10-month period (may 1997-february 1998) of 42 newborns with sepsis and/or meningitis. besides the clinical signs, a positive blood culture and/or a positive culture of cerebrospinal fluid was present in each case. gram-negat ... | 2004 | 14984170 |
| stability and activity of an enterobacter aerogenes-specific bacteriophage under simulated gastro-intestinal conditions. | a bacteriophage, designated uz1 and showing lytic activity against a clinically important strain (be1) of enterobacter aerogenes was isolated from hospital sewage. the stability and lytic activity against this strain under simulated gastro-intestinal conditions was evaluated. after addition of bacteriophage uz1 to a liquid feed at gastric ph 2, the phage was immediately inactivated and could not be recovered. however, by use of an antacid to neutralize stomach acidity, no significant changes in ... | 2004 | 14991251 |
| chemical cross-linking and mass spectrometric identification of sites of interaction for ured, uref, and urease. | synthesis of active klebsiella aerogenes urease requires four accessory proteins to generate, in a gtp-dependent process, a dinuclear nickel active site with the metal ions bridged by a carbamylated lysine residue. the ured and uref accessory proteins form stable complexes with urease apoprotein, comprised of urea, ureb, and urec. the sites of protein-protein interactions were explored by using homobifunctional amino group-specific chemical cross-linkers with reactive residues being identified b ... | 2004 | 14749331 |
| thiamine biosynthesis in escherichia coli: in vitro reconstitution of the thiazole synthase activity. | the biosynthesis of thiamine in escherichia coli requires the formation of an intermediate thiazole from tyrosine, 1-deoxy-d-xylulose-5-phosphate (dxp), and cysteine using at least six structural proteins, thifsgh, iscs, and thii. we describe for the first time the reconstitution of thiazole synthase activity using cell-free extracts and proteins derived from adenosine-treated e. coli 83-1 cells. the addition of adenosine or adenine to growing cultures of aerobacter aerogenes, salmonella typhimu ... | 2004 | 14757766 |
| phenazines and other redox-active antibiotics promote microbial mineral reduction. | natural products with important therapeutic properties are known to be produced by a variety of soil bacteria, yet the ecological function of these compounds is not well understood. here we show that phenazines and other redox-active antibiotics can promote microbial mineral reduction. pseudomonas chlororaphis pcl1391, a root isolate that produces phenazine-1-carboxamide (pcn), is able to reductively dissolve poorly crystalline iron and manganese oxides, whereas a strain carrying a mutation in o ... | 2004 | 14766572 |
| bacteriological quality of some pharmaceutical products marketed by drug vendors in uyo, nigeria. | bacteriological quality of some pharmaceutical products purchased from open markets, buses and drug stores in uyo metropolis was studied in order to determine the level of contamination of the drugs. the drug samples examined were tetracycline capsules, paracetamol tablets, ampicillin capsules, chloroquine tablets, chloroquine syrup, chloroquine injections, flagyl tablets and chloramphenicol tablets. the bacterial count ranged from 2.3x10(4) cfu/g to 4.4x10(4) cfu/g for the 16 samples of ampicil ... | 2004 | 17298130 |
| transmembrane transcriptional control (surface signalling) of the escherichia coli fec type. | the ferric citrate transport system of escherichia coli is the first example of a transcription initiation mechanism that starts at the cell surface. the inducer, ferric citrate, binds to an outer membrane transport protein, and without further transport elicits a signal that is transmitted across the outer membrane, the periplasm, and the cytoplasmic membrane into the cytoplasm. signal transfer across the three subcellular compartments is mediated by the outer membrane transport protein that in ... | 2004 | 16102597 |
| microorganisms and parasites in chronic infective diarrhea. | to reveal the pattern of microorganisms in chronic infective diarrhea cases. | 2004 | 15931720 |
| meningitis due to enterobacter aerogenes subsequent to resection of an acoustic neuroma and abdominal fat graft to the mastoid. | meningitis is an uncommon complication of neurosurgical procedures, with an incidence of 1.1% to 2.5%. although unusual, the frequency of nosocomial gram-negative meningitis appears to be increasing. gram-negative meningitis has been documented following disruption of the dura-arachnoid barrier secondary to trauma or surgery. the association of gram-negative bacillary meningitis with neurosurgical procedures was first reported in the 1940's. wolff et al. described the association between enterob ... | 2004 | 15798816 |
| vitreoscilla hemoglobin renders enterobacter aerogenes highly susceptible to heavy metals. | when expressed in heterologous microorganisms vitreoscilla hemoglobin (vhb) acts as oxygen storage and causes a higher oxygen uptake. in this study, the effect of this protein on growth, sensitivity and antioxidant properties of enterobacter aerogenes exposed to metal stress was investigated. the strain expressing vhb was more sensitive to mercury and cadmium as the minimal inhibitory concentration (mic) for these metals was up to 2-fold lower in this strain than the host and the recombinant str ... | 2004 | 15689114 |
| mutational analysis of beta-glucoside utilization in klebsiella aerogenes: evidence for the presence of multiple genetic systems. | 2004 | 15689631 | |
| the susceptibility of certain bacterial strains to imidazotriazine derivatives. | the synthesized imidazo[2,1-c]triazines were tested for their antimicrobial activity. microbiological screening tests conducted in relation to 54 strains of gram-positive and 52 strains of gram-negative bacteria, 6 strains of yeast-like fungi and 3 strains of moulds showed significant activity of compound 19 against 52 gram-negative bacterial strains. this compound was active against 16 strains of escherichia coli, 12 strains of pseudomonas, 10 strains of proteus spp., 8 strains of klebsiella pn ... | 2004 | 16146057 |
| bacterial species specificity in prou osmoinducibility and nptii and lacz expression. | reporter gene-based transcriptional fusions are increasingly being used to address questions in microbial ecology, with constitutively expressed fusions enabling microbe tracking and inducible fusions reporting the presence of environmental signals. to more readily apply this technology to a variety of bacterial species, we examined species specificity in the expression of three promoters of interest. a comparison of two potentially constitutive promoters, each fused to the reporter gene gfp, sh ... | 2004 | 16179797 |
| high-yield production of hydrogen by enterobacter aerogenes mutants with decreased alpha-acetolactate synthase activity. | to enhance hydrogen (h2) production from glucose by enterobacter aerogenes hu-101, two mutants, strains vp-1 and vp-2, with decreased alpha-acetolactate synthase activity, were isolated using the voges-proskauer (vp) test. in ph-uncontrolled batch culture, both mutants showed a lower 2,3-butanediol yield for the glucose consumed than that shown by the wild-type strain, although glucose remained in the medium after 12 h of culture. in the same cultures, compared to the h2 yield of 0.80 mol/mol-gl ... | 2004 | 16233620 |
| antibacterial activity of the fourth-generation fluoroquinolones gatifloxacin and moxifloxacin against ocular pathogens. | the ideal ophthalmic anti-infective exhibits broad-spectrum activity against gram-positive, gram-negative, and atypical bacterial species. these pathogens can cause potentially blinding infections such as keratitis and endophthalmitis, both of which are associated with ophthalmic surgery or traumatic injury. these infections often require aggressive antibacterial therapy, preferably with newer generations of antibiotics. in this study, minimal inhibitory concentration (mic) values for gatifloxac ... | 2003 | 14964344 |
| [purification and characterization of a uridine phosphorylase from enterobacter aerogenes eam-z1]. | a uridine phosphorylase(upase) was isolation from enterobacter aerogenes eam-z1 and purified by means of ammonium sulfate precipitation, deae-cellulose, phenyl-sepharose, deae-sepharose, fplc ion exchange, and sephacryl s-200 column chromatography. the purified upase showed homogeneity on the native polacrylamide gel electrophoresis. the upase is a trimer of 43 kd subunits. fifteen residues from the amino terminal end of upase were identified as mrmvdliatkrdgge. the isoelectric point was ph 4.46 ... | 2003 | 16279202 |
| effect of microorganism characteristics on leak size critical to predicting package sterility. | the effects of microorganism size and motility on the leak size critical to the sterility of a package, along with the imposed pressure required to initiate liquid flow for the critical leak size, were measured. pseudomonas fragi lacy-1052, bacillus atrophaeus atcc 49337, and enterobacter aerogenes atcc 29007 were employed to assess package sterility. one hundred twenty-six 7-mm-long microtubes with interior diameters of 5, 10, and 20 microm were used to simulate package defects. forty-two solid ... | 2003 | 14503733 |
| in vitro susceptibilities of gram-negative bacteria isolated from hospitalized patients in four european countries, canada, and the united states in 2000-2001 to expanded-spectrum cephalosporins and comparator antimicrobials: implications for therapy. | access to current antimicrobial agent surveillance data is an important prerequisite for the optimal management of patients with hospital-acquired infections. the present study used data collected in 2000 to 2001 from 670 laboratories in europe (france, germany, italy, and spain), canada, and the united states to report on the in vitro activities of ceftriaxone, cefotaxime, and comparative agents against >125,000 isolates of gram-negative bacteria from hospitalized patients. all but two isolates ... | 2003 | 14506014 |
| leitbakteria of microbial biofilm communities causing occlusion of biliary stents. | biliary stents inserted to relieve obstructive jaundice caused by biliary or pancreatic malignancies inevitably become occluded by microbial growth in the form of diverse microbial community biofilms. the scarce information available on these communities is based on cultivation methods, but such methods usually provide distorted overviews of community composition, so commonalities and differences in biliary stent communities are uncertain. we extracted dna and rna from the microbial communities ... | 2003 | 14510839 |
| effect of thorium on the growth and capsule morphology of bradyrhizobium. | the thorium effect on bradyrhizobium growth was assayed in liquid media. th4+ inhibited the growth of bradyrhizobium (chamaecytisus) bga-1, but this effect decreased in the presence of suspensions of live or dead bacterial cells. th4+ induced the formation of a gel-like precipitate when added to a dense suspension of b. (chamaecytisus) bga-1 cells. viable bradyrhizobium cells remained in suspension after precipitate formation. thorium was recovered in the precipitate, in which polysaccharide, li ... | 2003 | 14510845 |
| purification of an enterobacter aerogenes plasmid dna using mncl2 as compaction agent. | 2003 | 14511692 | |
| [prostate histopathology of nih category iv prostatitis detected by sextant prostate needle biopsy from the patients with high prostatic specific antigen]. | asymptomatic prostatitis is classified as category iv in nih classification of prostatitis syndrome (1999). no report concerning this category has been present. we investigated this category histopathologically and clinically, in order to clarify the histopathological distribution and its correlation to the clinical features, in this study. among 785 patients who were suspected prostate cancer because of their high prostatic specific antigen (psa) values and to have a sextant prostate needle bio ... | 2003 | 14515754 |
| are infections due to resistant pathogens associated with a worse outcome in critically ill patients? | to evaluate the outcome of critically ill patients infected with antimicrobial resistant microorganisms, and to analyse the factors involved in the development of antimicrobial resistance. | 2003 | 14556755 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2001--ii. gram-negative bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2001 were yearly evaluated and compared with those of other cephems, oxacephems and carbapenems. a total of 3,245 strains in 32 species of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis, escherichia coli, citrobacter freundii, citrobact ... | 2003 | 14567255 |
| extended-spectrum beta-lactamase-producing enterobacteriaceae in community and private health care centers. | in 1999, 39 of 2,599 isolates of the family enterobacteriaceae (1.5%) collected by eight private laboratories in the aquitaine region in france produced an extended-spectrum beta-lactamase (esbl). among these were 19 enterobacter aerogenes isolates; 8 klebsiella pneumoniae isolates; 6 escherichia coli isolates; 3 proteus mirabilis isolates; and 1 isolate each of serratia marcescens, morganella morganii, and providencia stuartii. esbl producers were isolated from 38 patients, including 33 residen ... | 2003 | 14576109 |
| meningitis due to enterobacter aerogenes subsequent to resection of acoustic neuroma and percutaneous endoscopic gastrostomy tube placement: a rare nosocomial event. | we present a case of meningitis after percutaneous endoscopic gastrostomy (peg) tube placement subsequent to acoustic neuroma resection and cranioplasty. four days following peg tube placement, the patient developed enterobacter aerogenes meningitis, requiring explantation of infected cranioplasty material. his condition subsequently improved. etiology and future intervention strategies are discussed. | 2003 | 14587946 |
| the importance of bi-digital o-ring test in the treatment of multiple hepatic abscesses: a case history. | the bi-digital o-ring test has been very useful in the identification of bacterial and viral infections, as well as other etiological agents, in difficult clinical cases. case report of a patient with multiple hepatic abscesses (pylephlebitis induced hepatic abscess is the most difficult abscess to treat), in which the etiological agent was suggested through bi-digital o-ring test with excellent clinical evolution after modification of previously ineffective multi anti-microbial treatment is pre ... | 2003 | 14998058 |
| occurrence & detection of ampc beta-lactamases at a referral hospital in karnataka. | ampc beta-lactamases confer resistance to a wide variety of beta-lactam drugs except for cefepime, cefpirome and carbapenems. they are known to be responsible for nosocomial outbreaks, therapeutic failures and multidrug resistance. although reported with increasing frequency the true rate of occurrence of these beta-lactamases in enterobacteriaceae is not known. hence the present study was undertaken to determine the occurrence of ampc enzymes among clinical isolates. | 2003 | 14748463 |
| protistan grazing analysis by flow cytometry using prey labeled by in vivo expression of fluorescent proteins. | selective grazing by protists can profoundly influence bacterial community structure, and yet direct, quantitative observation of grazing selectivity has been difficult to achieve. in this investigation, flow cytometry was used to study grazing by the marine heterotrophic flagellate paraphysomonas imperforata on live bacterial cells genetically modified to express the fluorescent protein markers green fluorescent protein (gfp) and red fluorescent protein (rfp). broad-host-range plasmids were con ... | 2003 | 14602649 |
| extended-spectrum beta-lactamase tem-24 in an aeromonas clinical strain: acquisition from the prevalent enterobacter aerogenes clone in france. | 2003 | 14638519 | |
| inoculum size influences bacterial cross contamination between surfaces. | many factors have been shown to influence bacterial transfer between surfaces, including surface type, bacterial species, moisture level, pressure, and friction, but the effect of inoculum size on bacterial transfer has not yet been established. bacterial cross contamination rates during performance of common food service tasks were previously determined in our laboratory using nalidixic acid-resistant enterobacter aerogenes. eight different transfer rates were determined, each involving a minim ... | 2003 | 14660365 |
| cysteine proteases and cell differentiation: excystment of the ciliated protist sterkiella histriomuscorum. | the process of excystment of sterkiella histriomuscorum (ciliophora, oxytrichidae) leads in a few hours, through a massive influx of water and the resorption of the cyst wall, from an undifferentiated resting cyst to a highly differentiated and dividing vegetative cell. while studying the nature of the genes involved in this process, we isolated three different cysteine proteases genes, namely, a cathepsin b gene, a cathepsin l-like gene, and a calpain-like gene. excystation was selectively inhi ... | 2003 | 14665458 |
| [post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria]. | as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, monobactams, and penicillins. changes in czop susceptibility among bacteria were also evaluated with the bacterial resistance ratio calculated from the breakpoint mic. twenty-five species (4,154 strains) of gram-negative bacteria were isolated from the clinical ... | 2003 | 14692381 |
| sanitary and bacteriological aspects of sewage treatment. | a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ... | 2003 | 15058814 |
| physicochemical characterization and antimicrobial properties of rhamnolipids produced by pseudomonas aeruginosa 47t2 ncbim 40044. | pseudomonas aeruginosa 47t2, grown in submerged culture with waste frying oil as a carbon source, produced a mixture of rhamnolipids with surface activity. up to 11 rhamnolipid homologs (rha-rha-c(8)-c(10); rha-c(10)-c(8)/rha-c(8)-c(10);rha-rha-c(8)-c(12:1); rha-rha-c(10)-c(10); rha-rha-c(10)-c(12:1); rha-c(10)-c(10); rha-rha-c(10)-c(12)/rha-rha-c(12)-c(10); rha-c(10)-c(12:1)/rha-c(12:1)-c(10); rha-rha-c(12:1)-c(12); rha-rha-c(10)-c(14:1); rha-c(10)-c(12)/rha-c(12)-c(10)) were isolated from cult ... | 2003 | 12474254 |
| the comparative effect of novel pelargonium essential oils and their corresponding hydrosols as antimicrobial agents in a model food system. | essential oils and their corresponding hydrosols, obtained after distillation of various scented pelargonium (geraniaceae) leaves were assessed for their antimicrobial activity in a model food system. both the essential oils and hydrosols were used at 1000 ppm in broccoli soup, previously inoculated with enterobacter aerogenes (at 10(5) cfu g(-1)) and staphylococcus aureus (at 10(4) cfu g(-1)). the results showed a complete inhibition of s. aureus in the broccoli soup by the essential oils of 's ... | 2003 | 12557249 |
| imipenem and expression of multidrug efflux pump in enterobacter aerogenes. | imipenem is often used to treat intensive care unit patients infected by enterobacter aerogenes, but it is leading to an increasing number of antibiotic resistant strains. clinical isolates and imipenem resistant variants presented a high level of resistance to beta-lactam antibiotic group and to chemically unrelated drugs. we report here that imipenem selects strains which contain active efflux pumps ejecting various unrelated antibiotics including quinolones, tetracycline, and chloramphenicol. ... | 2003 | 12589810 |
| effect of specific oxygen uptake rate on enterobacter aerogenes energetics: carbon and reduction degree balances in batch cultivations. | the effect of oxygen availability on the metabolism of enterobacter aerogenes ncimb 10102 was studied through batch fermentations of glucose performed increasing the specific oxygen uptake rate up to 72.7 mmol(o2) c-mol(dw) (-1) x h(-1). the final concentrations of fermentation products of this biosystem (2,3-butanediol, hydrogen, acetoin, formate, acetate, carbon dioxide, ethanol, lactate, succinate, and biomass) were utilized to check the use of simple carbon mass and reduction degree balances ... | 2003 | 12599264 |
| sequencing of 16s rdna of klebsiella: taxonomic relations within the genus and to other enterobacteriaceae. | the 16s rdnas of 20 strains of klebsiella were sequenced and used for construction of a phylogenetic tree together with already published enterobacteriaceae 16s rdna sequences. the taxonomy within the klebsiella genus, as reflected by the 16s rdna tree, was in agreement with existing dna-dna hybridisation and numerical taxonomy data, indicating that for klebsiella, 16s rdna sequencing is a valid method for identification and taxonomical purposes. five closely related clusters were found in the k ... | 2003 | 12635932 |
| overexpression and purification of the three components of the enterobacter aerogenes acra-acrb-tolc multidrug efflux pump. | the tripartite acra-acrb-tolc system is the major efflux pump of the nosocomial pathogen enterobacter aerogenes. acra is a trimeric periplasmic lipoprotein anchored in the inner membrane, acrb is an inner membrane transporter and tolc is a trimeric outer membrane channel. in order to reconstitute the acra-acrb-tolc system of e. aerogenes in artificial membranes, we overexpressed and purified the three proteins. the e. aerogenes acra, acrb and tolc open reading frames were individually inserted i ... | 2003 | 12651015 |
| characterization of clinical isolates of enterobacteriaceae from italy by the bd phoenix extended-spectrum beta-lactamase detection method. | production of extended-spectrum beta-lactamases (esbls) is an important mechanism of beta-lactam resistance in enterobacteriaceae: identification of esbls based on phenotypic tests is the strategy most commonly used in clinical microbiology laboratories. the phoenix esbl test (bd diagnostic systems, sparks, md.) is a recently developed automated system for detection of esbl-producing gram-negative bacteria. an algorithm based on phenotypic responses to a panel of cephalosporins (ceftazidime plus ... | 2003 | 12682131 |
| potential for detection of microorganisms and heavy metals in potable water using electronic nose technology. | studies have been carried out to determine the potential for the detection of different microbial species (enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa), alone and in the presence of low concentrations of different heavy metals (as, cd, pb and zn) in bottled, reverse osmosis (ro) and tap water, using an electronic nose. studies show that it is possible to discriminate control water samples from water contaminated with 0.5 ppm of a mixture of metals. the presence of heavy meta ... | 2003 | 12706588 |
| modification of outer membrane protein profile and evidence suggesting an active drug pump in enterobacter aerogenes clinical strains. | two clinical strains of enterobacter aerogenes that exhibited phenotypes of multiresistance to beta-lactam antibiotics, fluoroquinolones, chloramphenicol, tetracycline, and kanamycin were investigated. both strains showed a porin pattern different from that of a susceptible strain, with a drastic reduction in the amount of the major porin but with an apparently conserved normal structure (size and immunogenicity), together with overproduction of two known outer membrane proteins, ompx and lamb. ... | 2003 | 12709321 |
| surfactant proteins a and d inhibit the growth of gram-negative bacteria by increasing membrane permeability. | the pulmonary collectins, surfactant proteins a (sp-a) and d (sp-d), have been reported to bind lipopolysaccharide (lps), opsonize microorganisms, and enhance the clearance of lung pathogens. in this study, we examined the effect of sp-a and sp-d on the growth and viability of gram-negative bacteria. the pulmonary clearance of escherichia coli k12 was reduced in sp-a-null mice and was increased in sp-d-overexpressing mice, compared with strain-matched wild-type controls. purified sp-a and sp-d i ... | 2003 | 12750409 |
| correlation between bacterial haemoglobin gene (vgb) and aeration: their effect on the growth and alpha-amylase activity in transformed enterobacter aerogenes. | to evaluate the effects of bacterial haemoglobin on bacterial growth and alpha-amylase formation under different aeration conditions. | 2003 | 12752814 |
| the escherichia fergusonii iucabcd iuta genes are located within a larger chromosomal region similar to pathogenicity islands. | three strains of escherichia fergusonii (ef873, ef1496, ef939) of 50 strains tested produced the hydroxamate siderophore aerobactin. screening of a cosmid library of the strain ef873 chromosomal dna (in aerobactin nonproducing escherichia coli vcs257) for aerobactin production identified iucabcd and iuta gene orthologues. the predicted iucabcd and iuta proteins showed 59-65% identity to the corresponding proteins of shigella flexneri and e. coli. aerobactin molecules synthesized by e. fergusonii ... | 2003 | 12800494 |
| persistent silver disinfectant for the environmental control of pathogenic bacteria. | contaminated surfaces can act as a reservoir for pathogenic microorganisms and potentially exacerbate the risk of infection. surface disinfection and decontamination provide temporary amelioration against bacterial colonization. disinfected surfaces eventually become contaminated, thus, mitigating the benefit of the initial disinfection. it is hypothesized that to improve on the current state of the art, a disinfectant should not only immediately disinfect a surface but also provide persistent a ... | 2003 | 12806357 |
| the beta-glucoside genes of klebsiella aerogenes: conservation and divergence in relation to the cryptic bgl genes of escherichia coli. | the ability to metabolize aromatic beta-glucosides such as salicin and arbutin varies among members of the enterobacteriaceae. the ability of escherichia coli to degrade salicin and arbutin appears to be cryptic, subject to activation of the bgl genes, whereas many members of the klebsiella genus can metabolize these sugars. we have examined the genetic basis for beta-glucoside utilization in klebsiella aerogenes. the klebsiella equivalents of bglg, bglb and bglr have been cloned using the genom ... | 2003 | 12829297 |
| comparison of four antimicrobial susceptibility testing methods to determine the in vitro activities of piperacillin and piperacillin-tazobactam against clinical isolates of enterobacteriaceae and pseudomonas aeruginosa. | susceptibility to piperacillin was similar to that to piperacillin-tazobactam (<1% difference) for 6,938 isolates of enterobacter aerogenes and 13,954 isolates of enterobacter cloacae tested using a vitek system; for the same species, in contrast, susceptibility rates to piperacillin-tazobactam were 5.9 to 13.9% higher than to piperacillin using disk diffusion, microscan, and vitek 2 testing. unprecedented phenotypes (piperacillin susceptible and piperacillin-tazobactam intermediate; piperacilli ... | 2003 | 12843088 |
| modification of the double-disk test for detection of enterobacteriaceae producing extended-spectrum and ampc beta-lactamases. | detection of extended-spectrum beta-lactamases (esbls) in ampc-producing enterobacteriaceae is problematic. a modification of the double-disk test (mddt) has been developed for successful detection of esbls in gram-negative bacilli producing well-characterized beta-lactamases as well as 212 clinical isolates of enterobacter cloacae, enterobacter aerogenes, serratia marcescens, and citrobacter freundii. mddt accurately differentiated between esbl producers and derepressed chromosomal ampc mutants ... | 2003 | 12904422 |
| comparison of microbial adherence to antiseptic and antibiotic central venous catheters using a novel agar subcutaneous infection model. | an agar subcutaneous infection model (agar model), which simulates the rat subcutaneous infection model (rat model), was developed to assess the ability of antimicrobial catheters to resist microbial colonization. the catheters were implanted in the agar and rat models and the insertion sites were infected immediately or on day 7, 14 or 21 post-implantation. the catheters implanted in the agar model were transferred to fresh media one day before infection on day 7, 14 or 21. the efficacy of chlo ... | 2003 | 12917250 |
| molecular epidemiology of enterobacteriaceae producing extended-spectrum beta-lactamase in a french university-affiliated hospital. | we conducted a retrospective study to investigate the epidemiology of enterobacteriaceae producing extended-spectrum beta-lactamase (esble) in our hospital. we determined the occurrence of extended-spectrum beta-lactamase (esbl) in enterobacteriaceae over a 2-year period. we also characterised esbls by isoelectric focusing (ief) and investigated the epidemiological relatedness of eblse by pulsed field gel electrophoresis (pfge). during this period, 70 patients were colonised/infected with one or ... | 2003 | 12927952 |
| extended-spectrum beta-lactamases in enterobacteriaceae in buenos aires, argentina, public hospitals. | resistance to extended-spectrum cephalosporins is often associated with plasmid encoded extended spectrum beta-lactamases (esbl). in order to evaluate the prevalence and diversity of esbls in enterobacteria in our city, a 1-month-period survey was carried out from april to may 2000. extended-spectrum-cephalosporin-resistant strains, isolated from inpatient clinical specimens other than stools, were collected among 17 participating hospitals. from a total of 427 enterobacterial strains that were ... | 2003 | 12936986 |
| outbreak of tem-24-producing enterobacter aerogenes in a spanish hospital. | organisms encoding multidrug resistance genes are becoming increasingly prevalent. during a 2-month period (december, 2000, to january, 2001), 83 consecutive isolates of enterobacter spp. were collected in our microbiology department. antibiotic susceptibility was determined using the vitek ii automatic system. we selected strains with decreased susceptibility to extended-spectrum cephalosporins. the double-disk potentiation test was positive in 10 of these strains, indicating the presence of ex ... | 2003 | 12959409 |
| characterization of blacmy-10 a novel, plasmid-encoded ampc-type beta-lactamase gene in a clinical isolate of enterobacter aerogenes. | we report the description of a novel plasmid-encoded ampc beta-lactamase gene (blacmy-10) from enterobacter aerogenes k9911729 that was isolated from a patient suffering from pneumonia in south korea. | 2003 | 12969288 |
| evaluation of oxoid combination discs for detection of extended-spectrum beta-lactamases. | we evaluated the reliability of cefpirome/clavulanate (cd04) compared with ceftazidime/clavulanate (cd02) and cefotaxime/clavulanate (cd03) oxoid combination discs for the detection of extended-spectrum beta-lactamases (esbl) in several enterobacteriaceae isolates, including enterobacter spp. | 2003 | 12972449 |
| isolation of a negative control mutant of the nitrogen assimilation control protein, nac, in klebsiella aerogenes. | a negative control mutant of the nitrogen assimilation control protein, nac, has been isolated. mutants with the leucine at position 111 changed to a nonhydrophobic residue activate transcription from hut and ure promoters, but fail to repress gdha expression. this failure does not result from failure to bind to either of the two sites required for gdha repression, but the binding at those sites is altered in the mutant. it appears that the nac negative control mutants fail to form the complex s ... | 2003 | 12511519 |
| comparative in vitro activity of three fluoroquinolones against clinical isolates by e test. | the in vitro susceptibility of levofloxacin, ciprofloxacin and moxifloxacin against several gram-positive and gram-negative clinical isolates was tested by e test. we found that the mic(50) and mic(90) values against all members of the enterobacteriaceae family except serratia were <0.5 mg/l for ciprofloxacin and levofloxacin (mic range 0.006-32 mg/l) based on the in vitro susceptibility data. the susceptibility rates for ciprofloxacin and levofloxacin were more than 85% for escherichia coli, ci ... | 2003 | 12886053 |
| genetic engineering of enterobacter aerogenes with the vitreoscilla hemoglobin gene: cell growth, survival, and antioxidant enzyme status under oxidative stress. | hemoglobins in unicellular organisms, like the one here in the bacterium vitreoscilla, have greater chemical reactivity than their homologues in multicellular organisms. they can catalyze redox reactions and may protect cells against oxidative stress. the ability of vitreoscilla hemoglobin to complement deficiencies of terminal cytochrome oxidases in escherichia coli also suggests that this hemoglobin can receive electrons during respiration. in this study, a recombinant strain of enterobacter a ... | 2003 | 12892849 |
| thanatin activity on multidrug resistant clinical isolates of enterobacter aerogenes and klebsiella pneumoniae. | efflux pumps protect bacterial cells by ejecting intracellular toxic molecules such as antibiotics, detergents and defensins that have penetrated the cell envelope. some of these efflux pumps recognise structurally unrelated compounds (mdr pump) and account for the resistance of some organisms to two or more agents. it would be of interest to identify molecules that are able to circumvent the problems created by multidrug resistance phenotypes during antibiotic therapy. we have studied the activ ... | 2003 | 13678832 |
| 4-alkoxy and 4-thioalkoxyquinoline derivatives as chemosensitizers for the chloramphenicol-resistant clinical enterobacter aerogenes 27 strain. | enterobacter aerogenes is a gram-negative bacteria frequently responsible for nosocomial respiratory tract infections. strains resistant to chloramphenicol are frequently isolated. alkoxy and thio-alkoxyquinolines have a potential to act as chemosensitizers that would render multi-drug-resistant (mdr) bacterial infections susceptible to antibiotics to which they were originally resistant. several new quinoline derivatives have been prepared, characterized and studied for their ability to increas ... | 2003 | 13678833 |
| evaluation of antibacterial efficacy of drugs for urinary tract infections by genotyping based on pulsed-field gel electrophoresis (pfge). | we attempted to determine whether the same bacterium isolated from patients with urinary tract infections (uti) before and after treatment with antimicrobial agents was of the same strain ("persisted") or of a different strain ("changed"). furthermore, to verify the effectiveness of antimicrobial agents for uti, we investigated whether bacterial strains could be classified as persisted or changed based on their electrophoretic patterns in pulsed-field gel electrophoresis (pfge). we examined eigh ... | 2002 | 12525898 |
| biosynthesis of pyridoxine in saccharomyces cerevisiae--origin of the pyridoxine nitrogen atom differs under anaerobic and aerobic conditions. | the amide nitrogen atom of glutamine is incorporated into pyridoxine in four eukaryotes (i.e., emericella nidulans, mucor racemosus, neurospora crassa and saccharomyces cerevisiae) and two prokaryotes (i.e., staphylococcus aureus and bacillus subtilis). however, in the prokaryotes pseudomonas putida, enterobacter aerogenes and escherichia coli, it is the nitrogen atom of glutamate that is incorporated into pyridoxine (j nutr sci vitaminol (2000) 46, 55-57). as these results were from experiments ... | 2002 | 12775110 |
| extended spectrum beta-lactamase mediated resistance in urinary tract isolates of family enterobacteriaceae. | extended spectrum beta-lactamases (esbl) hydrolyse expanded spectrum cephalosporins like ceftazidime, cefotaxime and monobactam aztreonam. esbl producing bacteria may not be detectable in the routine disk diffusion susceptibility tests leading to inappropriate use of antibiotics and treatment failures. as information on esbl producers causing urinary tract infection (uti) is not available from our country, the occurrence of esbl producing strains causing uti was studied as also the differences b ... | 2002 | 12674828 |
| mar operon involved in multidrug resistance of enterobacter aerogenes. | we determined the sequence of the entire marrab operon in enterobacter aerogenes. it is functionally and structurally analogous to the escherichia coli operon. the overexpression of e. aerogenes mara induces a multidrug resistance phenotype in a susceptible strain, demonstrated by a noticeable resistance to various antibiotics, a decrease in immunodetected porins, and active efflux of norfloxacin. | 2002 | 11897595 |
| tem derivative-producing enterobacter aerogenes strains: dissemination of a prevalent clone. | tem-24 (caz-6) extended-spectrum beta-lactamase (esbl) was detected in 1988 in clermont-ferrand, france, in klebsiella pneumoniae (bla(tem-24)) and enterobacter aerogenes (bla(tem-24b)), and since 1994, a tem-24-producing e. aerogenes clonal strain has been observed elsewhere in the country. to determine if the spread of this clonal strain was restricted to tem-24-producing e. aerogenes strains, 84 e. aerogenes strains (non-tem/shv-producing strains, tem-1- or -2-producing strains, and different ... | 2002 | 11897606 |
| epidemiology of extended-spectrum beta-lactamase-producing enterobacter isolates in a spanish hospital during a 12-year period. | fifteen enterobacter clinical isolates (11 enterobacter cloacae isolates, 3 enterobacter aerogenes isolates, and 1 enterobacter gergoviae isolate), representing 0.4% of all enterobacter isolates recovered in our hospital from 1989 to 2000, were suspected of harboring an extended-spectrum beta-lactamase (esbl). these isolates were recovered from 14 different patients. esbls were transferred by conjugation into an escherichia coli recipient strain. pulsed-field gel electrophoresis (pfge) revealed ... | 2002 | 11923338 |
| evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies. | phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ... | 2002 | 11931166 |
| prevalence of extended spectrum beta-lactamases among enterobacteriaceae: an italian survey. | the prevalence of extended-spectrum beta-lactamase (esbl) production by consecutive non-repeated isolates of enterobacteriaceae was determined over a 6-month period. a total of 8015 strains were isolated from ten italian laboratories and 509 (6.3%) of these were designated esbl producers from the results of a double-disk synergy test. escherichia coli was the most isolated microrganism, followed by klebsiella pneumoniae and proteus mirabilis. providencia stuartii (28.1%) was the most frequently ... | 2002 | 11932144 |
| risk assessment of hand washing efficacy using literature and experimental data. | this study simulated factors that influence the levels of bacteria on foodservice workers' hands. relevant data were collected from the scientific literature and from laboratory experiments. literature information collected included: initial bacterial counts on hands and water faucet spigots, bacterial population changes during hand washing as effected by soap type, sanitizing agent, drying method, and the presence of rings. experimental data were also collected using enterobacter aerogenes as a ... | 2002 | 11934038 |
| [enterobacteria isolated from cockroaches (periplaneta americana) captured in a brazilian hospital]. | to isolate and identify microorganisms from cockroaches that were captured in a public hospital and to test the antimicrobial susceptibility of these microorganisms. | 2002 | 11939120 |
| novel alkylsulfatases required for biodegradation of the branched primary alkyl sulfate surfactant 2-butyloctyl sulfate. | recent reports show that contrary to common perception, branched alkyl sulfate surfactants are readily biodegradable in standard biodegradability tests. we report here the isolation of bacteria capable of biodegrading 2-butyloctyl sulfate and the identification of novel enzymes that initiate the process. enrichment culturing from activated sewage sludge yielded several strains capable of growth on 2-butyloctyl sulfate. of these, two were selected for further study and identified as members of th ... | 2002 | 11772605 |
| polyphosphate produced in recombinant escherichia coli confers mercury resistance. | an escherichia coli strain was generated by fusion of a mera-deleted broad-spectrum mer operon from pseudomonas k-62 with a bacterial polyphosphate kinase gene (ppk) from klebsiella aerogenes in vector puc119. a large amount of the ppk-specified polyphosphate was identified in the mercury-induced bacterium with the fusion plasmid designated pmkb18 but not in the cells without mercury induction. these results suggest that the synthesis of polyphosphate as well as the expression of the mer genes i ... | 2002 | 11958934 |