Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| electrochemical dna sensor for neisseria meningitidis detection. | meningitis sensor based on nucleic acid probe of neisseria meningitidis has been fabricated by immobilization of 5'-thiol end labeled single stranded deoxyribonucleic acid probe (ssdna-sh) onto gold (au) coated glass electrode. this ssdna-sh/au electrode hybridized with the genomic dna (g-dsdna/au) and amplified dna (pcr-dsdna/au) has been characterized using atomic force microscopy (afm), fourier transforms infrared spectroscopy (ft-ir) and electrochemical techniques. the ssdna-sh/au electrode ... | 2010 | 20472423 |
| frequency and rate of pilin antigenic variation of neisseria meningitidis. | the rates of pilin antigenic variation (av) of two strains of neisseria meningitidis were determined using an unbiased dna sequencing assay. strain mc58 underwent pilin av at a rate similar to that of n. gonorrhoeae strain ms11 but lower than that of n. gonorrhoeae strain fa1090. pilin av was undetectable in strain fam18. | 2010 | 20472803 |
| the elephant never forgets; piloting a chlamydia and gonorrhea retesting reminder postcard in an std clinic setting. | we examined the number and proportion of persons retesting and reinfected with chlamydia and/or gonorrhea 3 to 4 months after initial infection in one new york city sexually transmitted disease clinic using a reminder postcard compared to clinics not using this reminder. retesting increased; however, the proportion reinfected was lower during the intervention. | 2010 | 20473247 |
| the structure of a reduced form of oxyr from neisseria meningitidis. | survival of the human pathogen, neisseria meningitidis, requires an effective response to oxidative stress resulting from the release of hydrogen peroxide by cells of the human immune system. in n. meningitidis, expression of catalase, which is responsible for detoxifying hydrogen peroxide, is controlled by oxyr, a redox responsive lysr-type regulator. oxyr responds directly to intracellular hydrogen peroxide through the reversible formation of a disulphide bond between c199 and c208 in the regu ... | 2010 | 20478059 |
| thermostable formulations of a hepatitis b vaccine and a meningitis a polysaccharide conjugate vaccine produced by a spray drying method. | thermostable vaccines promise to simplify the logistics of vaccine distribution and expand the immunization coverage. in this study, a pilot-scale spray drying process was developed and used to produce glassy state formulations of a recombinant hepatitis b (hepb) vaccine containing aluminum adjuvant and neisseria meningitidis a (mena) protein-polysaccharide conjugate vaccine, representing two common types of subunit vaccines in use today: the spray-dried hepb vaccine formulations were stable for ... | 2010 | 20478345 |
| 2010 fifa world cup south africa: travel health issues and new options for protection against meningococcal disease. | the public health implications of large crowds gathering at a range of key global events should never be underestimated. this is especially the case with the upcoming 2010 fifa world cup south africa programme where thousands of local and travelling spectators, players and officials from all over the world will be present. although meningococcal disease contracted whilst actually travelling is relatively rare, any travel health risk assessment should involve consideration of potential exposure t ... | 2010 | 20478511 |
| the oligosaccharide of gonococcal lipooligosaccharide contains several epitopes that are recognized by human antibodies. | recently, we isolated human igg from normal human sera (nhs) using lipooligosaccharide (los) from gonococcal strain jw31r as an affinity ligand. we provided evidence that the oligosaccharide (os) moiety of los was immunogenic in humans and that nhs contains functional antibodies that bind to the branched os. the present study aimed to identify bactericidal antibodies that bind to partial core os structures or their adjacent sites expressed in the 3,4-branched and 2,3:3,4-dibranched neisserial lo ... | 2010 | 20479085 |
| multiple pilus motors cooperate for persistent bacterial movement in two dimensions. | in various bacterial species surface motility is mediated by cycles of type iv pilus motor elongation, adhesion, and retraction, but it is unclear whether bacterial movement follows a random walk. here we show that the correlation time of persistent movement in neisseria gonorrhoeae increases with the number of pili. the unbinding force of individual pili from the surface f=10 pn was considerably lower than the stalling force f>100 pn, suggesting that density, force, and adhesive properties of t ... | 2010 | 20482147 |
| cervicovaginal fluid and semen block the microbicidal activity of hydrogen peroxide produced by vaginal lactobacilli. | h2o2 produced by vaginal lactobacilli is believed to protect against infection, and h2o2-producing lactobacilli inactivate pathogens in vitro in protein-free salt solution. however, cervicovaginal fluid (cvf) and semen have significant h2o2-blocking activity. | 2010 | 20482854 |
| spectinomycin in the treatment of gonorrhoea. | 2010 | 20483105 | |
| improved omv vaccine against neisseria meningitidis using genetically engineered strains and a detergent-free purification process. | the use of detergent-extracted outer membrane vesicles (omvs) is an established approach for development of a multivalent pora vaccine against n. meningitidis serogroup b. selective removal of lipopolysaccharide (lps) decreases toxicity, but promotes aggregation and narrows the immune response. detergent-free omv vaccines retain all lps, which preserves the native vesicle structure, but result in high toxicity and lower yield. the present study assessed the effects of gene mutations that attenua ... | 2010 | 20483197 |
| structural and biochemical characterization of free methionine-r-sulfoxide reductase from neisseria meningitidis. | a new family of methionine-sulfoxide reductase (msr) was recently described. the enzyme, named frmsr, selectively reduces the r isomer at the sulfoxide function of free methionine sulfoxide (met-r-o). the frmsrs belong to the gaf fold family. they represent the first gaf domain to show enzymatic activity. two other msr families, msra and msrb, were already known. msra and msrb reduce free met-s-o and met-r-o, respectively, but exhibit higher catalytic efficiency toward met-o within a peptide or ... | 2010 | 20489204 |
| identification and characterization of important residues in the catalytic mechanism of cmp-neu5ac synthetase from neisseria meningitidis. | sialylated oligosaccharides, present on mammalian outer-cell surfaces, play vital roles in cellular interactions and some bacteria are able to mimic these structures to evade their host's immune system. it would be of great benefit to the study of infectious and autoimmune diseases and cancers, to understand the pathway of sialylation in detail to enable the design and production of inhibitors and mimetics. sialylation occurs in two stages, the first to activate sialic acid and the second to tra ... | 2010 | 20491913 |
| measuring antigen-specific bactericidal responses to a multicomponent vaccine against serogroup b meningococcus. | serum bactericidal activity using human complement is the basis for established correlates of protection against invasive meningococcal disease. during the development of multicomponent protein-based vaccines against meningococcus b, it is necessary to measure antigen-specific bactericidal responses. this is not straightforward because each strain may be killed by antibodies to multiple antigens. we characterized a large panel of strains and, using a competitive inhibition sba, we identified fou ... | 2010 | 20493284 |
| genetic structure of neisseria meningitidis populations. | 2010 | 20493968 | |
| role of citrate and phosphate anions in the mechanism of iron(iii) sequestration by ferric binding protein: kinetic studies of the formation of the holoprotein of wild-type fbpa and its engineered mutants. | ferric binding protein a (fbpa) plays a central role in the iron acquisition processes of pathogenic neisseria gonorrheae, neisseria meningitidis, and haemophilus influenzae. fbpa functions as an iron shuttle within the periplasmic space of these gram-negative human pathogens. iron is picked up by fbpa at the periplasmic aspect of the outer membrane with concomitant acquisition of a synergistic anion. here we report the kinetics and mechanisms involved with loading of iron(iii) into iron-free fb ... | 2010 | 20496864 |
| multiplex polymerase chain reaction-based reverse line blot hybridization assay to detect common genital pathogens. | the objective of the present paper is to develop and apply a multiplex polymerase chain reaction (mpcr) based reverse line blot (rlb) hybridization assay to facilitate the diagnosis of genital infections by detection of seven recognized or putative genital pathogens (neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, ureaplasma parvum, mycoplasma genitalium, mycoplasma hominis and trichomonas vaginalis). species-specific biotin-labelled primer pairs were used in a single mpcr ... | 2010 | 20498099 |
| risk of chlamydia trachomatis infection during pregnancy: effectiveness of guidelines-based screening in identifying cases. | our goal was to define the risk factors for chlamydia trachomatis (ct) infection among pregnant women at a large urban medical centre. in a retrospective study, clinical records at a us maternity unit from july 2005 through february 2008 were reviewed. the study population included all pregnant women with a singleton newborn of at least 20 weeks gestation and antenatal care information. logistic regression was used to analyse the association between a positive ct test and demographic, behavioura ... | 2010 | 20498110 |
| [the effectiveness and safety of vaccine against meningococci group c conjugated with tetanus toxoid co-administrated with different vaccines--literature review]. | review of articles on efficacy and safety of co-administration of neisseria meningitidis serogroup c -tetanus toxoid conjugate vaccine (neisvac-c) with other vaccines. | 2010 | 20499668 |
| the effectiveness of patient-delivered partner therapy and chlamydial and gonococcal reinfection in san francisco. | patient-delivered partner therapy (pdpt) has been evaluated in randomized trials. no analysis has examined the impact of pdpt once implemented programmatically. | 2010 | 20502392 |
| etiology and sti/hiv coinfections among patients with urethral and vaginal discharge syndromes in south africa. | this study was undertaken to establish the etiology of the male urethral discharge (muds) and vaginal discharge (vds) syndromes, to determine the prevalence of other sexually transmitted infections (sti) and human immunodeficiency virus (hiv) coinfections, and to examine associations between stis and hiv serostatus among sti patients in south africa. | 2010 | 20502394 |
| neisseria meningitidis opc invasin binds to the sulphated tyrosines of activated vitronectin to attach to and invade human brain endothelial cells. | the host vasculature is believed to constitute the principal route of dissemination of neisseria meningitidis (nm) throughout the body, resulting in septicaemia and meningitis in susceptible humans. in vitro, the nm outer membrane protein opc can enhance cellular entry and exit, utilising serum factors to anchor to endothelial integrins; but the mechanisms of binding to serum factors are poorly characterised. this study demonstrates that nm opc expressed in acapsulate as well as capsulate bacter ... | 2010 | 20502634 |
| [epidemiological, clinical and laboratory characteristics of bacterial meningitis in adult patients]. | to determine the epidemiological, clinical and laboratory characteristics of adult bacterial meningitis in the czech republic, including complications and causes of death. | 2010 | 20503157 |
| epidemiology and meningococcal serogroup distribution in the united states. | 2010 | 20507868 | |
| oip5 is a highly expressed potential therapeutic target for colorectal and gastric cancers. | previously, we reported that overexpression of opa (neisseria gonorrhoeae opacity-associated)-interacting protein 5 (oip5) caused multi-septa formation and growth defects, both of which are considered cancer-related phenotypes. to evaluate oip5 as a possible cancer therapeutic target, we examined its expression level in 66 colorectal cancer patients. oip5 was upregulated about 3.7-fold in tumors and over 2-fold in 58 out of 66 colorectal cancer patients. knockdown of oip5 expression by small int ... | 2010 | 20510019 |
| endotracheal tube biofilm inoculation of oral flora and subsequent colonization of opportunistic pathogens. | endotracheal (et) tubes accumulate a biofilm during use, which can harbor potentially pathogenic microorganisms. the enrichment of pathogenic strains in the biofilm may lead to ventilator-associated pneumonia (vap) with an increased morbidity rate in intensive care units. we used quantitative pcr (qpcr) and gene surveys targeting 16s rrna genes to quantify and identify the bacterial community to detect fastidious/nonculturable organisms present among extubated et tubes. we collected eight et tub ... | 2010 | 20510651 |
| reduced susceptibility to ceftriaxone in neisseria gonorrhoeae is associated with mutations g542s, p551s and p551l in the gonococcal penicillin-binding protein 2. | reduced susceptibility to extended-spectrum cephalosporins in neisseria gonorrhoeae has, to date, been associated with three alterations: a mosaic pena allele encoding the penicillin-binding protein 2 (pbp2); a-del-mtrr, an adenine deletion in the mtrr promoter; and penb, comprising mutated alleles of porbib. in this study, we examined an association between reduced susceptibility to ceftriaxone and additional mutations in gonococcal pbp2. | 2010 | 20511367 |
| neisseria gonorrhoeae fa1090 carries genes encoding two classes of vsr endonucleases. | a very short patch repair system prevents mutations resulting from deamination of 5-methylcytosine to thymine. the vsr endonuclease is the key enzyme of this system, providing sequence specificity. we identified two genes encoding vsr endonucleases v.ngoaxiii and v.ngoaxiv from neisseria gonorrhoeae fa1090 based on dna sequence similarity to genes encoding vsr endonucleases from other bacteria. after expression of the gonococcal genes in escherichia coli, the proteins were biochemically characte ... | 2010 | 20511499 |
| [correlation between predominant pi genotypes, g120/a121 mutations and drug resistance.]. | to analyze the predominant genotypes of outer membrane porin i (pi) and to determine the correlation between g120 as well as a121 mutations in pi proteins and drug resistance in neisseria gonorrhoeae isolates in the local area. | 2010 | 20513293 |
| meningococcal group a, c, y and w-135 conjugate vaccine. | in february 2010, a quadrivalent conjugate vaccine (menveo; novartis vaccines and diagnostics) was approved by the us fda to prevent invasive meningococcal disease caused by neisseria meningitidis serogroups a, c, y and w-135 in people aged 11-55 years. | 2010 | 20514064 |
| emergence of new virulent neisseria meningitidis serogroup c sequence type 11 isolates in france. | in france, there have been variations in the incidence of invasive meningococcal infection due to serogroup c isolates. infection peaks were observed in 1992 and 2003 that involved isolates of phenotypes c:2a:p1.5,2 and/or c:2a:p1.5, which belong to the sequence type 11 (st-11) clonal complex. we report an emergence of isolates belonging to the st-11 clonal complex since 2003. these isolates displayed a new phenotype, c:2a:p1.7,1, caused infections that occurred as clusters, and were associated ... | 2010 | 20515410 |
| absence of neisseria meningitidis from throat swabs of kuwaiti pilgrims after returning from the hajj. | to investigate whether or not neisseria meningitidis was present in the throat of kuwaiti pilgrims after returning from the hajj. | 2010 | 20516711 |
| [characteristics of microflora of laryngeal mucosa in healthy subjects and patients with cicatrical stenosis of the larynx]. | the authors present data on the occurrence, density, and species composition of microorganisms isolated from pharyngeal mucosa of 40 healthy subjects and 55 patients with cicatrical stenosis of the larynx. the total density of microbial populations in control subjects was estimated at 2,672+/-614 cfu/ml compared with 17,510+/-1,983 cfu/ml in patients with cicatrical stenosis of the larynx unrelated to malignant neoplastic growth and 19,375+/-2,103 cfu/ml in patients who had experienced surgical ... | 2010 | 20517273 |
| molecular analysis of two novel neisseria gonorrhoeae virulent components: the macrophage infectivity potentiator and the outer membrane protein a. | molecular analyses of mip and ompa genes were performed on 20 neisseria gonorrhoeae isolates. the genes were present with a high degree of conservation in all strains. sera from patients with urethritis or disseminated gonococcal infections were able to recognize the purified neisseria gonorrhoeae macrophage infectivity potentiator (ng-mip) and neisseria gonorrhoeae outer membrane protein a (ng-ompa). | 2010 | 20518279 |
| the implications of vaccines for prevention of bacterial meningitis. | conjugate vaccines now exist that can protect against some types of bacterial meningitis (haemophilus influenzae type b, neisseria meningitidis group c and seven serotypes of streptococcus pneumoniae). to broaden the protection against meningitis, new vaccines are needed. this article reviews new uses of the established vaccines and the new meningitis vaccines in development. | 2010 | 20173637 |
| experimental studies of pneumococcal meningitis. | this thesis summarizes experimental meningitis research conducted at statens serum institut in collaboration with the copenhagen hiv programme and the danish research centre for magnetic resonance between 2001 and 2007. previous experimental studies had shown that the host inflammatory response in invasive infections contributed significantly to an extremely poor outcome despite initiation of efficient antimicrobial chemotherapy. consequently, we aimed to investigate and clarify how the course o ... | 2010 | 20175949 |
| entry of neisseria meningitidis into mammalian cells requires the src family protein tyrosine kinases. | neisseria meningitidis, the causative agent of meningitis and septicemia, is able to attach to and invade a variety of cell types. in a previous study we showed that entry of n. meningitidis into human brain microvascular endothelial cells (hbmec) is mediated by fibronectin bound to the outer membrane protein opc, which forms a molecular bridge to alpha 5 beta 1-integrins. this interaction results in cytoskeletal remodeling and uptake of the bacteria. in this study we identified and characterize ... | 2010 | 20176789 |
| risk factors and algorithms for chlamydial and gonococcal cervical infections in women attending family planning clinics in thailand. | to identify risk factors associated with and evaluate algorithms for predicting chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) cervical infections in women attending family planning clinics in thailand. | 2010 | 20178541 |
| o-acetylation of peptidoglycan in gram-negative bacteria: identification and characterization of peptidoglycan o-acetyltransferase in neisseria gonorrhoeae. | the ape2 gene encoding a hypothetical o-acetylpeptidoglycan esterase was amplified from genomic dna of neisseria gonorrhoeae fa1090 and cloned to encode either the full-length protein or a truncated version lacking its hypothetical signal sequence. expression trials revealed that production of the full-length version possessing either an n-terminal or c-terminal his(6) tag was toxic to escherichia coli transformants and that the host rapidly degraded the small amount of protein that was produced ... | 2010 | 20178982 |
| a dual role of extracellular dna during biofilm formation of neisseria meningitidis. | major pathogenic clonal complexes (cc) of neisseria meningitidis differ substantially in their point prevalence among healthy carriers. we show that frequently carried pathogenic cc (e.g. sequence type st-41/44 cc and st-32 cc) depend on extracellular dna (edna) to initiate in vitro biofilm formation, whereas biofilm formation of cc with low point prevalence (st-8 cc and st-11 cc) was edna-independent. for initial biofilm formation, a st-32 cc type strain, but not a st-11 type strain, utilized e ... | 2010 | 20180907 |
| a neonate in septic shock. | 2010 | 20164076 | |
| association of meningococcal phenotypes and genotypes with clinical characteristics and mortality of meningitis in children. | neisseria meningitidis meningitis represents approximately one-half of the meningococcal cases in french children. to explore the contribution of bacterial typing in improving the management of cases, we aimed to describe clinical characteristics and mortality of meningococcal meningitis in children reported to the multicenter survey system, gpip/activ, in association with phenotypes/genotypes of bacterial isolates. | 2010 | 20168263 |
| detoxified endotoxin vaccine (j5dlps/omp) protects mice against lethal respiratory challenge with francisella tularensis schus4. | francisella tularensis is a category a select agent. j5dlps/omp is a novel vaccine construct consisting of detoxified, o-polysaccharide side chain-deficient, lipopolysaccharide non-covalently complexed with the outer membrane protein of n. meningitidis group b. immunization elicits high-titer polyclonal antibodies specific for the highly-conserved epitopes expressed within the glycolipid core that constitutes gram-negative bacteria (e.g., f. tularensis). mice immunized intranasally with j5dlps/o ... | 2010 | 20170768 |
| recovery in parents of children and adolescents who survived septic shock caused by neisseria meningitidis: a cross-sectional study. | to assess psychological distress, styles of coping and disease-related psychosocial limitations in parents of children and adolescents who survived meningococcal septic shock (mss) 4-16 years ago. research methodology: an exploratory design using standardised questionnaires and interviews. | 2010 | 20171889 |
| development of a novel pcr method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections. | the objective of this study was to develop a novel polymerase chain reaction (pcr) method to comprehensively analyze salivary bacterial flora. | 2010 | 20171911 |
| genomic content of neisseria species. | the physical properties of most bacterial genomes are largely unexplored. we have previously demonstrated that the strict human pathogen neisseria gonorrhoeae is polyploid, carrying an average of three chromosome copies per cell and only maintaining one pair of replication forks per chromosome (d. m. tobiason and h. s. seifert, plos biol. 4:1069-1078, 2006). we are following up this initial report to test several predictions of the polyploidy model of gonococcal chromosome organization. we demon ... | 2010 | 20172999 |
| [neisseria meningitidis arthritis in 4-year-old child]. | a case presentation of primary meningococcal arthritis. the patient, a four-year and four-month old boy was admitted with fever, pain in the left shoulder and adenitis. the patient received relevant treatment with penicillin and arthroscopic irrigation. the patient recovered completely. neisseria meningitidis was isolated from the shoulder's synovial fluid. | 2010 | 20184826 |
| antimicrobial susceptibility of neisseria gonorrhoeae isolates determined by the agar dilution, disk diffusion and etest methods: comparison of results using gc agar and chocolate agar. | although the use of gc agar for determining neisseria gonorrhoeae antimicrobial susceptibilities is suggested by clinical and laboratory standard institute (clsi) guidelines, chocolate agar is still used in some regions owing to its low cost and availability. to determine the differences in susceptibilities determined using gc and chocolate agars, 163 non-duplicate n. gonorrhoeae isolates were tested. minimum inhibitory concentrations (mics) and percent susceptibilities determined using the gc a ... | 2010 | 20188518 |
| characterization of meningococcal serogroup b outer membrane vesicle vaccines from strain 44/76 after growth in different media. | in this study, we evaluated the effect of the growth medium on the composition and immunogenicity of meningococcal outer membrane vesicle (omv) vaccines after cultivation of the norwegian serogroup b 44/76 vaccine strain in either frantz' or modified catlin-6 media (mc.6m). differential proteomic analysis revealed that 97% of the omv proteins maintained the same levels in the two preparations. however, a number of differentially expressed proteins, including tdfh, opca, omp nmb0088, hypothetical ... | 2010 | 20188677 |
| the influence of is1301 in the capsule biosynthesis locus on meningococcal carriage and disease. | previously we have shown that insertion of is1301 in the sia/ctr intergenic region (igr) of serogroup c neisseria meningitidis (menc) isolates from spain confers increased resistance against complement-mediated killing. here we investigate the significance of is1301 in the same location in n. meningitidis isolates from the uk. pcr and sequencing was used to screen a collection of more than 1500 meningococcal carriage and disease isolates from the uk for the presence of is1301 in the igr. is1301 ... | 2010 | 20195528 |
| the crystal structure of ngo0477 from neisseria gonorrhoeae reveals a novel protein fold incorporating a helix-turn-helix motif. | 2010 | 20196080 | |
| immunogenicity and some safety features of a vegf-based cancer therapeutic vaccine in rats, rabbits and non-human primates. | we have developed a cancer vaccine candidate (hereafter denominated cigb-247), based on recombinant modified human vascular endothelial growth factor (vegf) as antigen, and the adjuvant vssp (very small sized proteoliposomes of neisseria meningitidis outer membrane). in mice, previous work of our group had shown that vaccination with cigb-247 extended tumor-take time, slowed tumor growth, and increased animal survival. immunization elicited anti-human and murine vegf-neutralizing antibodies, and ... | 2010 | 20197134 |
| population structure and capsular switching of invasive neisseria meningitidis isolates in the pre-meningococcal conjugate vaccine era--united states, 2000-2005. | a quadrivalent meningococcal conjugate vaccine (mcv4) was licensed in the united states in 2005; no serogroup b vaccine is available. neisseria meningitidis changes its capsular phenotype through capsular switching, which has implications for vaccines that do not protect against all serogroups. | 2010 | 20199241 |
| the moonlighting protein fructose-1, 6-bisphosphate aldolase of neisseria meningitidis: surface localization and role in host cell adhesion. | fructose-1, 6-bisphosphate aldolases (fba) are cytoplasmic glycolytic enzymes, which despite lacking identifiable secretion signals, have also been found localized to the surface of several bacteria where they bind host molecules and exhibit non-glycolytic functions. neisseria meningitidis is an obligate human nasopharyngeal commensal, which has the capacity to cause life-threatening meningitis and septicemia. recombinant native n. meningitidis fba was purified and used in a coupled enzymic assa ... | 2010 | 20199602 |
| vaccine preventability of meningococcal clone, greater aachen region, germany. | emergence of serogroup b meningococci of clonal complex sequence type (st) 41/44 can cause high levels of disease, as exemplified by a recent epidemic in new zealand. multiplication of annual incidence rates (3.1 cases/100,000 population) of meningococcal disease in a defined german region, the city of aachen and 3 neighboring countries (greater aachen) prompted us to investigate and determine the source and nature of this outbreak. using molecular typing and geographic mapping, we analyzed 1,14 ... | 2010 | 20202422 |
| high prevalence of mutations in quinolone-resistance-determining regions and mtrr loci in polyclonal neisseria gonorrhoeae isolates at a tertiary hospital in southern taiwan. | the emergence of multidrug-resistant neisseria gonorrhoeae is a great challenge in controlling gonorrhea. this study was conducted to survey the prevalence of molecular mechanisms of antimicrobial resistance among 45 clinical isolates of n. gonorrhoeae collected at a university hospital in southern taiwan during 1999-2004. | 2010 | 20206836 |
| enzymatic synthesis of gb3 and igb3 ceramides. | gb3 and igb3 are physiologically important trihexosylceramides with a terminal alpha-d-galp-(1-->4)-beta-d-galp- and alpha-d-galp-(1-->3)-beta-d-galp sequence, respectively. in particular igb3 is attracting considerable attention as it is believed to serve as a ligand for natural killer t cells. whether or not igb3 is present in humans and which enzyme might be responsible for its synthesis is at present a matter of lively debate. in the current investigation we evaluated human blood group b gal ... | 2010 | 20206917 |
| meeting report: a celebration of the work of professor tony hart, liverpool, united kingdom, 7 march 2009. | over 300 delegates participated in this scientific meeting to celebrate the career of the late professor tony hart, who was head of department of medical microbiology, university of liverpool, from 1986 until his death in september 2007. the meeting, which was opened by professor james stewart (head, school of infection and host defence, university of liverpool) and closed by professor bernard brabin (head of the child health group, liverpool school of tropical medicine), captured some of the ma ... | 2010 | 20207719 |
| who should get the meningococcal vaccine? | 2010 | 20207987 | |
| meningitis in chad, niger and nigeria: 2009 epidemic season. | 2010 | 20210043 | |
| requirement of nmb0065 for connecting assembly and export of sialic acid capsular polysaccharides in neisseria meningitidis. | capsule expression in neisseria meningitidis is encoded by the cps locus comprised of genes required for biosynthesis and surface translocation. located adjacent to the gene encoding the polysialyltransferase in serogroups expressing sialic acid-containing capsule, nmb0065 is likely a member of the cps locus, but it is not found in serogroups a or x that express non-sialic acid capsules. to further understand its role in cps expression, nmb0065 mutants were created in the serogroups b, c and y s ... | 2010 | 20215001 |
| seminal plasma hiv levels in men with asymptomatic sexually transmitted infections. | the effect of asymptomatic sexually transmitted urethral infections on human immunodeficiency virus (hiv) rna viral load in semen is poorly defined. we studied five such patients. those on antiretrovirals (n = 2) had lower seminal plasma viral loads (spvl) (2.11 and 1.98 log(10) copies/ml) than those not on antiretrovirals (n = 3) (2.27-3.78 log(10) copies/ml). one patient who was not taking antiretrovirals had a 94% decline in spvl after treatment of asymptomatic chlamydia trachomatis urethriti ... | 2010 | 20215629 |
| broad spectrum respiratory pathogen analysis of throat swabs from military recruits reveals interference between rhinoviruses and adenoviruses. | military recruits experience a high incidence of febrile respiratory illness (fri), leading to significant morbidity and lost training time. adenoviruses, group a streptococcus pyogenes, and influenza virus are implicated in over half of the fri cases reported at recruit training center clinics, while the etiology of the remaining cases is unclear. in this study, we explore the carriage rates and disease associations of adenovirus, enterovirus, rhinovirus, streptococcus pneumoniae, haemophilus i ... | 2010 | 20217405 |
| the epidemiology of meningococcal disease and the impact of vaccines. | neisseria meningitidis causes endemic meningococcal disease worldwide. serogroups b and c are responsible for the majority of cases of meningococcal disease in europe, serogroups b, c and y cause most disease in the americas, and serogroups a, c and w135 predominate in asia and africa. polysaccharide vaccines against meningococcal serogroups a, c, y and w135 have been available for several decades, but have been little used due to poor immunogenicity in young children and minimal effects on naso ... | 2010 | 20218857 |
| licensure of a meningococcal conjugate vaccine (menveo) and guidance for use - advisory committee on immunization practices (acip), 2010. | on february 19, 2010, the food and drug administration (fda) licensed a quadrivalent meningococcal conjugate vaccine, menacwy-crm (menveo, novartis vaccines and diagnostics). menacwy-crm is licensed as a single dose for use among persons aged 11-55 years. the advisory committee on immunization practices (acip) reviewed data from prelicensure clinical trials on the safety and immunogenicity of menacwy-crm. this report summarizes the approved indications for menacwy-crm and provides guidance from ... | 2010 | 20224545 |
| using a point-of-dispensing clinic for prophylaxis of meningococcal disease. | a point-of-dispensing clinic was held to distribute ciprofloxacin prophylaxis when 2 high school students were reported to the health department with invasive meningococcal disease. of more than 3,100 school staff and students in attendance, 2,861 received prophylaxis. a survey was administered to students 2 weeks postclinic to better understand the motivations for clinic attendance and to quantify side effects of oral 500-mg ciprofloxacin prophylaxis. data collected included reasons for attenda ... | 2010 | 20230232 |
| [diagnosis and treatment of acute uncomplicated urethritis of men: guidelines from the french committee of infectious disease of the french association of urology (ciafu)]. | resistance progression of the neisseria gonorrhoeae to quinolones and the decreasing sensitivity to cephalosporin implicate to actualise the guidelines for managing urethritis. we present the guidelines from the committee of infectious diseases of the french association of urology to manage acute urethritis. | 2010 | 20230939 |
| efficient synthesis of 6'-sialyllactose, 6,6'-disialyllactose, and 6'-kdo-lactose by metabolically engineered e. coli expressing a multifunctional sialyltransferase from the photobacterium sp. jt-ish-224. | we have previously reported the efficient conversion of lactose into 3'-sialyllactose by high cell density cultures of a genetically engineered escherichia coli strain expressing the neisseria meningitidis gene for alpha-(2-->3)-sialyltransferase [fierfort, n.; samain, e. j. biotechnol. 2008, 134, 261-265.]. first attempts to use a similar strategy to produce 6'-sialyllactose with a strain expressing alpha-(2-->6)-sialyltransferase from the photobacterium sp. jt-ish-224 led to the production of ... | 2010 | 20231015 |
| anaerobic metabolism occurs in the substratum of gonococcal biofilms and may be sustained in part by nitric oxide. | neisseria gonorrhoeae is the etiologic agent of gonorrhea, which has been among the most frequently reported communicable diseases in the united states since 1960. women frequently do not exhibit symptoms, which can lead to chronic infection. n. gonorrhoeae readily forms biofilms over abiotic surfaces, over primary and transformed cervical epithelial cells, and over cervical tissues in vivo. biofilms are often associated with chronic infection, which suggests a link between biofilm formation and ... | 2010 | 20231417 |
| opacity proteins increase neisseria gonorrhoeae fitness in the female genital tract due to a factor under ovarian control. | the neisserial opacity (opa) proteins are a family of antigenically distinct outer membrane proteins that undergo phase-variable expression. opa(+) variants of neisseria gonorrhoeae strain fa1090 are selected in a cyclical pattern from the lower genital tract of estradiol-treated mice. here we show that cyclical recovery of opa(+) gonococci does not occur in ovariectomized mice; therefore, the reproductive cycle plays a role in the selection kinetics in vivo. as predicted by the selection patter ... | 2010 | 20100859 |
| polyaniline/carbon nanotubes platform for sexually transmitted disease detection. | polyaniline/carbon nanotubes composite (pani-cnt) electrochemically deposited onto indium-tin-oxide (ito) coated glass plate has been utilized for neisseria gonorrhoeae detection by immobilizing 5'-amino-labeled neisseria gonorrhoeae probe (adna) using glutaraldehyde as a cross-linker. pani-cnt/ito and adna-glu-pani-cnt/ito electrodes have been characterized using scanning electron microscopy (sem), fourier transform infrared (ft-ir) spectroscopy, cyclic voltammetry (cv), and differential pulse ... | 2010 | 20101643 |
| fever as nature's engine. part of beneficial host response? | 2010 | 20103514 | |
| hiv-1 and stis prevalence and risk factors of miners in mining districts of yunnan, china. | to assess hiv/sexually transmitted infection (sti) prevalence and associated risk factors among miners in yunnan, china. | 2010 | 20104111 |
| serum bactericidal antibody response 1 year after meningococcal polysaccharide vaccination of patients with common variable immunodeficiency. | some patients with common variable immunodeficiency (cvid) can generate an antibody response following vaccination with neisseria meningitidis polysaccharide, but the duration of this protection is unknown. in this study, serum bactericidal antibody (sba) responses to serogroup c n. meningitidis of 23 patients with cvid and 23 sex- and age-matched controls were measured 1 year after vaccination with the plain a/c meningococcal polysaccharide vaccine. the fold rise in serum bactericidal antibody ... | 2010 | 20106999 |
| coincorporation of lpxl1 and pagl mutant lipopolysaccharides into liposomes with neisseria meningitidis opacity protein: influence on endotoxic and adjuvant activity. | wild-type lipopolysaccharide (lps) of neisseria meningitidis normally contains six acyl chains. penta-acylated lps forms were generated through inactivation of the lpxl1 gene or through the expression of the bordetella bronchiseptica pagl gene in n. meningitidis. the resulting lps species, designated lpxl1 lps and pagl lps, respectively, display reduced endotoxic activity compared to wild-type lps. here, we determined the adjuvant potential of pagl lps by comparison with the broadly used lpxl1 l ... | 2010 | 20107001 |
| critical role of th17 responses in a murine model of neisseria gonorrhoeae genital infection. | host immune responses, including the characteristic influx of neutrophils, against neisseria gonorrhoeae are poorly understood; adaptive immunity is minimal and non-protective. we hypothesize that n. gonorrhoeae selectively elicits th17-dependent responses, which trigger innate defense mechanisms, including neutrophils and antimicrobial proteins, that it can resist. we found that n. gonorrhoeae induced the production of interleukin-17 (il-17) in mouse t-cells and th17-inducing cytokines in mouse ... | 2010 | 20107432 |
| domain iii of the envelope protein as a dengue vaccine target. | a dengue vaccine should induce long-lasting, simultaneous protection to the four dengue viruses while avoiding the immune enhancement of viral infection. domain iii of the dengue envelope protein has been implicated in receptor binding, and is also the target of specific neutralizing antibodies. domain iii has emerged as a promising region for a subunit vaccine candidate. here, we review the current state of knowledge on vaccine candidates based on domain iii. due to the results obtained concern ... | 2010 | 20109025 |
| neisseria meningitidis serogroup w135, china. | 2010 | 20113581 | |
| neisseria meningitidis serogroup x sequence type 2888, italy. | 2010 | 20113588 | |
| serogroup b meningococcal vaccines-an unfinished story. | most invasive meningococcal disease in developed countries is caused by neisseria meningitidis with a serogroup b capsule. however, despite availability of vaccines for other serogroups since the 1960s, no serogroup b vaccine exists. in this review we look at the development of serogroup b vaccines over the past 40 years. outer membrane vesicle vaccines have been successfully used to control geographically isolated epidemics, but most have not been highly immunogenic in young children or provide ... | 2010 | 20113980 |
| the c-terminal regulatory domain is required for catalysis by neisseria meningitidis alpha-isopropylmalate synthase. | alpha-isopropylmalate synthase (alpha-ipms) catalyses the first committed step in leucine biosynthesis in many pathogenic bacteria, including neisseria meningitidis. this enzyme (nmeipms) has been purified, characterised, and compared to alpha-ipms proteins from other bacteria. nmeipms is a homodimer which catalyses the condensation of alpha-ketoisovalerate (alpha-kiv) and acetyl coenzyme a (accoa), and is inhibited by leucine. nmeipms can use alternate alpha-ketoacids as substrates and, in cont ... | 2010 | 20117081 |
| neonatal intrauterine infection with neisseria meningitidis b. | 2010 | 20118079 | |
| new mutation in 23s rrna gene associated with high level of azithromycin resistance in neisseria gonorrhoeae. | 2010 | 20123998 | |
| acute bacterial meningitis in infants and children. | bacterial meningitis continues to be an important cause of mortality and morbidity in neonates and children throughout the world. the introduction of the protein conjugate vaccines against haemophilus influenzae type b, streptococcus pneumoniae, and neisseria meningitidis has changed the epidemiology of bacterial meningitis. suspected bacterial meningitis is a medical emergency and needs empirical antimicrobial treatment without delay, but recognition of pathogens with increasing resistance to a ... | 2010 | 20129147 |
| long-term evolution of antigen repertoires among carried meningococci. | most studies of bacterial pathogen populations have been based on isolates collected from individuals with disease, or their contacts, over short time periods. for commensal organisms that occasionally cause disease, such as neisseria meningitidis, however, the analysis of isolates from long-term asymptomatic carriage is necessary to elucidate their evolution and population structure. here, we use mathematical models to analyse the structuring and dynamics of three vaccine-candidate antigens amo ... | 2010 | 20129981 |
| cellular and molecular biology of neisseria meningitidis colonization and invasive disease. | the human species is the only natural host of neisseria meningitidis, an important cause of bacterial meningitis globally, and, despite its association with devastating diseases, n. meningitidis is a commensal organism found frequently in the respiratory tract of healthy individuals. to date, antibiotic resistance is relatively uncommon in n. meningitidis isolates but, due to the rapid onset of disease in susceptible hosts, the mortality rate remains approx. 10%. additionally, patients who survi ... | 2010 | 20132098 |
| [the trick of the meningococcus]. | 2010 | 20132764 | |
| neisseria meningitidis gna2132, a heparin-binding protein that induces protective immunity in humans. | gna2132 is a neisseria meningitidis antigen of unknown function, discovered by reverse vaccinology, which has been shown to induce bactericidal antibodies in animal models. here we show that this antigen induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease. the protein binds heparin in vitro through an arg-rich region and this property correlates with increased survival of the unencapsulated bacterium in human serum. furthermore, two protease ... | 2010 | 20133713 |
| evaluation of aptima combo 2 for cross-reactivity with oropharyngeal neisseria species and other microorganisms. | 2010 | 20138032 | |
| increased expression of the type iv secretion system in piliated neisseria gonorrhoeae variants. | neisseria gonorrhoeae produces a type iv secretion system that secretes chromosomal dna. the secreted dna is active in the transformation of other gonococci in the population and may act to transfer antibiotic resistance genes and variant alleles for surface antigens, as well as other genes. we observed that gonococcal variants that produced type iv pili secreted more dna than variants that were nonpiliated, suggesting that the process may be regulated. using microarray analysis, we found that a ... | 2010 | 20139191 |
| the phasevarion: phase variation of type iii dna methyltransferases controls coordinated switching in multiple genes. | in several host-adapted pathogens, phase variation has been found to occur in genes that encode methyltransferases associated with type iii restriction-modification systems. it was recently shown that in the human pathogens haemophilus influenzae, neisseria gonorrhoeae and neisseria meningitidis phase variation of a type iii dna methyltransferase, encoded by members of the mod gene family, regulates the expression of multiple genes. this novel genetic system has been termed the 'phasevarion' (ph ... | 2010 | 20140025 |
| epidemiological profile of meningococcal disease in the united states. | neisseria meningitidis is a leading cause of bacterial meningitis and other serious infections worldwide. the epidemiological profile of n. meningitidis is highly changeable, with great differences in disease incidence and serogroup distribution. six serogroups (namely serogroups a, b, c, w-135, x, and y) are responsible for most cases of meningococcal disease worldwide; the epidemiological profile of disease caused by each serogroup is unique. no vaccine is available for endemic disease caused ... | 2010 | 20144015 |
| prevention of meningococcal disease: current use of polysaccharide and conjugate vaccines. | invasive meningococcal disease (imd), although uncommon, is difficult to diagnose and can be rapidly fatal, even in healthy young persons. imd is cyclic, and serogroups responsible for disease vary by age group, although the prevalence of the serogroups changes over time and by geographical location. two quadrivalent vaccines are licensed in the united states to prevent imd caused by serogroups a, c, y, and w-135, and the us centers for disease control and prevention recommends routine vaccinati ... | 2010 | 20144016 |
| review of meningococcal group b vaccines. | no broadly effective vaccines are available for prevention of group b meningococcal disease, which accounts for >50% of all cases. the group b capsule is an autoantigen and is not a suitable vaccine target. outer-membrane vesicle vaccines appear to be safe and effective, but serum bactericidal responses in infants are specific for a porin protein, pora, which is antigenically variable. to broaden protection, outer-membrane vesicle vaccines have been prepared from >1 strain, from mutants with >1 ... | 2010 | 20144017 |
| african meningitis belt pneumococcal disease epidemiology indicates a need for an effective serotype 1 containing vaccine, including for older children and adults. | pneumococcal conjugate vaccine strategies in gavi-eligible countries are focusing on infant immunization but this strategy may not be optimal in all settings. we aimed to collect all available population based data on pneumococcal meningitis throughout life in the african meningitis belt and then to model overall meningitis risk to help inform vaccine policy. | 2010 | 20146815 |
| neisseria gonorrhoeae enhances hiv-1 infection of primary resting cd4+ t cells through tlr2 activation. | sexually transmitted infections increase the likelihood of hiv-1 transmission. we investigated the effect of neisseria gonorrheae (gonococcus [gc]) exposure on hiv replication in primary resting cd4(+) t cells, a major hiv target cell during the early stage of sexual transmission of hiv. gc and tlr2 agonists, such as peptidylglycan (pgn), pam(3)csk(4), and pam(3)c-lip, a gc-derived synthetic lipopeptide, but not tlr4 agonists including lps or gc lipooligosaccharide enhanced hiv-1 infection of pr ... | 2010 | 20147631 |
| the secretome of campylobacter concisus. | a higher prevalence of campylobacter concisus and higher levels of igg antibodies specific to c. concisus in crohn's disease patients than in controls were recently detected. in this study, 1d and 2d gel electrophoresis coupled with ltq ft-ms and qstar tandem ms, respectively, were performed to characterize the secretome of a c. concisus strain isolated from a crohn's disease patient. two hundred and one secreted proteins were identified, of which 86 were bioinformatically predicted to be secret ... | 2010 | 20148967 |
| variation and molecular evolution of hmbr, the neisseria meningitidis haemoglobin receptor. | meningococcal disease caused by serogroup b neisseria meningitidis remains an important health problem in many parts of the world, and there are currently no comprehensive vaccines. poor immunogenicity, combined with immunological identity to human sialic acids, have hindered the development of a serogroup b conjugate vaccine, resulting in the development of alternative vaccine candidates, including many outer-membrane protein (omp)-based formulations. however, the design of protein-based mening ... | 2010 | 20150237 |
| algorithmic assessment of vaccine-induced selective pressure and its implications on future vaccine candidates. | posttrial assessment of a vaccine's selective pressure on infecting strains may be realized through a bioinformatic tool such as parsimony phylogenetic analysis. following a failed gonococcal pilus vaccine trial of neisseria gonorrhoeae, we conducted a phylogenetic analysis of pilin dna and predicted peptide sequences from clinical isolates to assess the extent of the vaccine's effect on the type of field strains that the volunteers contracted. amplified pilin dna sequences from infected vaccine ... | 2010 | 20150957 |
| a cross-sectional study of reported symptoms for sexually transmissible infections among female sex workers in papua new guinea. | sexually transmissible infections (stis) are common in female sex workers (fsws), most of which are asymptomatic and therefore under-reported. our aim was to determine the sensitivity and specificity of reported symptoms obtained via questionnaire augmented with leukocyte esterase (le) urine dipstick test for the detection of chlamydia trachomatis (ct), neisseria gonorrhea (ng) and trichomonas vaginalis (tv) detected using polymerase chain reaction (pcr). | 2010 | 20152100 |