Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cheese microbial risk assessments - a review. | cheese is generally considered a safe and nutritious food, but foodborne illnesses linked to cheese consumption have occurred in many countries. several microbial risk assessments related to listeria monocytogenes, staphylococcus aureus, and escherichia coli infections, causing cheese-related foodborne illnesses, have been conducted. although the assessments of microbial risk in soft and low moisture cheeses such as semi-hard and hard cheeses have been accomplished, it has been more focused on t ... | 2016 | 26950859 |
| program and abstracts from the canadian digestive diseases week™ 2016. | 2016 | 27563641 | |
| maternal weaning modulates emotional behavior and regulates the gut-brain axis. | evidence shows that nutritional and environmental stress stimuli during postnatal period influence brain development and interactions between gut and brain. in this study we show that in rats, prevention of weaning from maternal milk results in depressive-like behavior, which is accompanied by changes in the gut bacteria and host metabolism. depressive-like behavior was studied using the forced-swim test on postnatal day (pnd) 25 in rats either weaned on pnd 21, or left with their mother until p ... | 2016 | 26903212 |
| reduced soluble rage is associated with disease severity of axonal guillain-barré syndrome. | soluble receptor for advanced glycation end products (srage) is an anti-inflammatory factor that mitigates the proinflammatory effects of high mobility group box 1 (hmgb1). the aim of this study was to investigate whether guillain-barré syndrome (gbs)-related inflammation are mediated by srage and hmgb1. we measured serum srage, hmgb1, il-6, and tnf-α levels in 86 patients with gbs and analysed associations between srage or hmgb1 and clinical variables in these subjects. in addition, we determin ... | 2016 | 26902096 |
| structural modeling of the flagellum ms ring protein flif reveals similarities to the type iii secretion system and sporulation complex. | the flagellum is a large proteinaceous organelle found at the surface of many bacteria, whose primary role is to allow motility through the rotation of a long extracellular filament. it is an essential virulence factor in many pathogenic species, and is also a priming component in the formation of antibiotic-resistant biofilms. the flagellum consists of the export apparatus on the cytosolic side; the basal body and rotor, spanning the bacterial membrane(s) and periplasm; and the hook-filament, t ... | 2016 | 26925337 |
| inflammation in irritable bowel syndrome: myth or new treatment target? | low-grade intestinal inflammation plays a key role in the pathophysiology of irritable bowel syndrome (ibs), and this role is likely to be multifactorial. the aim of this review was to summarize the evidence on the spectrum of mucosal inflammation in ibs, highlighting the relationship of this inflammation to the pathophysiology of ibs and its connection to clinical practice. we carried out a bibliographic search in medline and the cochrane library for the period of january 1966 to december 2014, ... | 2016 | 26900287 |
| cell wall anchoring of the campylobacter antigens to lactococcus lactis. | campylobacter jejuni is the most frequent cause of human food-borne gastroenteritis and chicken meat is the main source of infection. recent studies showed that broiler chicken immunization against campylobacter should be the most efficient way to lower the number of human infections by this pathogen. induction of the mucosal immune system after oral antigen administration should provide protective immunity to chickens. in this work we tested the usefulness of lactococcus lactis, the most extens ... | 2016 | 26925040 |
| distribution and genetic profiles of campylobacter in commercial broiler production from breeder to slaughter in thailand. | poultry and poultry products are commonly considered as the major vehicle of campylobacter infection in humans worldwide. to reduce the number of human cases, the epidemiology of campylobacter in poultry must be better understood. therefore, the objective of the present study was to determine the distribution and genetic relatedness of campylobacter in the thai chicken production industry. during june to october 2012, entire broiler production processes (i.e., breeder flock, hatchery, broiler fa ... | 2016 | 26886590 |
| spotyping: fast and accurate in silico mycobacterium spoligotyping from sequence reads. | spotyping is a fast and accurate program for in silico spoligotyping of mycobacterium tuberculosis isolates from next-generation sequencing reads. this novel method achieves high accuracy for reads of both uniform and varying lengths, and is about 20 to 40 times faster than spolpred. spotyping also integrates the function of producing a report summarizing associated epidemiological data from a global database of all isolates having the same spoligotype. spotyping is freely available at: https:// ... | 2016 | 26883915 |
| bacterial multidrug efflux pumps: much more than antibiotic resistance determinants. | bacterial multidrug efflux pumps are antibiotic resistance determinants present in all microorganisms. with few exceptions, they are chromosomally encoded and present a conserved organization both at the genetic and at the protein levels. in addition, most, if not all, strains of a given bacterial species present the same chromosomally-encoded efflux pumps. altogether this indicates that multidrug efflux pumps are ancient elements encoded in bacterial genomes long before the recent use of antibi ... | 2016 | 27681908 |
| utilization of carrageenan, citric acid and cinnamon oil as an edible coating of chicken fillets to prolong its shelf life under refrigeration conditions. | the present study was conducted to determine efficacy of edible coating of carrageenan and cinnamon oil to enhance the shelf life of chicken meat stored under refrigeration conditions. | 2016 | 27051203 |
| occurrence of campylobacter spp. in poultry meat at retail and processing plants' levels in central italy. | human campylobacteriosis remains the most commonly reported gastrointestinal disease in europe and campylobacter (c.) jejuni and c. coli are the two species most frequently involved in such foodborne disease. based on the sampling plan established in the region of lazio (central italy) the aim of our work was to investigate the occurrence of campylobacter spp. in poultry meat preparations collected by the local veterinary authority at retail shops and processing plants. we also observed whether ... | 2016 | 27800429 |
| development of a specific monoclonal antibody for the quantification of artemisinin in artemisia annua and rat serum. | artemisinin, extracted from artemisia annua, and its derivatives are important frontline antimalarials. to produce specific antibodies for the detection and quantification of artemisinin, artemisinin was transformed to 9-hydroxyartemisinin by microbial fermentation, which was used to prepare a 9-succinate artemisinin hapten for conjugation with ovalbumin. a monoclonal antibody (mab), designated as 3h7a10, was selected from hybridoma cell lines which showed high specificity to artemisinin. no com ... | 2016 | 26822789 |
| the epigenomic landscape of prokaryotes. | dna methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes. studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation, but the prevalence and properties of such non-restriction-associated methylation systems remain poorly understood. here, we used single molecule, real-time sequencing to map dna modifications including m6a, m4c, and m5c across the genomes of 230 diverse bacterial and archaeal ... | 2016 | 26870957 |
| human campylobacteriosis in luxembourg, 2010-2013: a case-control study combined with multilocus sequence typing for source attribution and risk factor analysis. | campylobacteriosis has increased markedly in luxembourg during recent years. we sought to determine which campylobacter genotypes infect humans, where they may originate from, and how they may infect humans. multilocus sequence typing was performed on 1153 campylobacter jejuni and 136 c. coli human strains to be attributed to three putative animal reservoirs (poultry, ruminants, pigs) and to environmental water using the asymmetric island model. a nationwide case-control study (2010-2013) for do ... | 2016 | 26860258 |
| zika: the origin and spread of a mosquito-borne virus. | to describe the temporal and geographical distribution of zika virus infection and associated neurological disorders, from 1947 to 1 february 2016, when zika became a public health emergency of international concern (pheic). | 2016 | 27708473 |
| molecular mechanisms and potential clinical applications of campylobacter jejuni cytolethal distending toxin. | cytolethal distending toxin (cdt), a genotoxin produced by campylobacter jejuni, is composed of three subunits: cdta, cdtb, and cdtc. cdtb is a dnase that causes dna double-strand breaks (dsb) in the nucleus resulting in cell cycle arrest at the g2/m stage and apoptosis. cdta and cdtc bind to cholesterol-rich microdomains on the cytoplasmic membrane, a process required for the delivery of cdtb to cells. although a unique motif associated with cholesterol-binding activity has been identified in o ... | 2016 | 26904508 |
| tuning of hemes b equilibrium redox potential is not required for cross-membrane electron transfer. | in biological energy conversion, cross-membrane electron transfer often involves an assembly of two hemesb the hemes display a large difference in redox midpoint potentials (δem_b), which in several proteins is assumed to facilitate cross-membrane electron transfer and overcome a barrier of membrane potential. here we challenge this assumption reporting on hemebligand mutants of cytochromebc1in which, for the first time in transmembrane cytochrome, one natural histidine has been replaced by lysi ... | 2016 | 26858251 |
| genomic features of environmental and clinical vibrio parahaemolyticus isolates lacking recognized virulence factors are dissimilar. | vibrio parahaemolyticus is a bacterial pathogen that can cause illness after the consumption or handling of contaminated seafood. the primary virulence factors associated with v. parahaemolyticus illness are thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh). however, clinical strains lacking tdh and trh have recently been isolated, and these clinical isolates are poorly understood. to help understand the emergence of clinical tdh- and trh-negative isolates, a genomic approach w ... | 2016 | 26637607 |
| immunomodulatory and anti-inflammatory activities of chicken cathelicidin-2 derived peptides. | host defence peptides and derived peptides are promising classes of antimicrobial and immunomodulatory lead compounds. for this purpose we examined whether chicken cathelicidin-2 (cath-2)-derived peptides modulate the function and inflammatory response of avian immune cells. using a chicken macrophage cell line (hd11) we found that full-length cath-2 dose-dependently induced transcription of chemokines cxcli2/il-8, mcp-3 and ccli4/rantes, but not of pro-inflammatory cytokine il-1β. in addition, ... | 2016 | 26848845 |
| the two-component system cpxra negatively regulates the locus of enterocyte effacement of enterohemorrhagic escherichia coli involving σ(32) and lon protease. | enterohemorrhagic escherichia coli (ehec) is a significant cause of serious human gastrointestinal disease worldwide. ehec strains contain a pathogenicity island called the locus of enterocyte effacement (lee), which encodes virulence factors responsible for damaging the gut mucosa. the cpx envelope stress response of e. coli is controlled by a two-component system (tcs) consisting of a sensor histidine kinase (cpxa) and a cytoplasmic response regulator (cpxr). in this study, we investigated the ... | 2016 | 26904510 |
| evolutionary genomics suggests that chev is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex. | escherichia coli and salmonella enterica are models for many experiments in molecular biology including chemotaxis, and most of the results obtained with one organism have been generalized to another. while most components of the chemotaxis pathway are strongly conserved between the two species, salmonella genomes contain some chemoreceptors and an additional protein, chev, that are not found in e. coli. the role of chev was examined in distantly related species bacillus subtilis and helicobacte ... | 2016 | 26844549 |
| multilocus sequence typing and antimicrobial resistance of campylobacter jejuni isolated from dairy calves in austria. | human campylobacteriosis is primarily associated with poultry but also cattle. in this study, 55 campylobacter jejuni strains isolated from 382 dairy calves' feces were differentiated by multilocus sequence typing and tested for antimicrobial resistance. the most prevalent sequence type (st) was st883 (20.0%), followed by st48 (14.5%), and st50 (9.1%). in contrast to st48 and st50, st883 has rarely been described in cattle previously. furthermore, risk factor analysis was performed for the prese ... | 2016 | 26870027 |
| recent development of mass spectrometry and proteomics applications in identification and typing of bacteria. | identification and typing of bacteria occupy a large fraction of time and work in clinical microbiology laboratories. with the certification of some ms platforms in recent years, more applications and tests of ms-based diagnosis methods for bacteria identification and typing have been created, not only on well-accepted maldi-tof-ms-based fingerprint matches, but also on solving the insufficiencies of maldi-tof-ms-based platforms and advancing the technology to areas such as targeted ms identific ... | 2016 | 26751976 |
| to what extent is faims beneficial in the analysis of proteins? | high field asymmetric waveform ion mobility spectrometry (faims), also known as differential ion mobility spectrometry, is emerging as a tool for biomolecular analysis. in this article, the benefits and limitations of faims for protein analysis are discussed. the principles and mechanisms of faims separation of ions are described, and the differences between faims and conventional ion mobility spectrometry are detailed. protein analysis is considered from both the top-down (intact proteins) and ... | 2016 | 26843211 |
| helicobacter pylori colonization in nepal; assessment of prevalence and potential risk factors in a hospital-based patient cohort. | helicobacter pylori, a gram-negative bacterium, can cause gastritis, peptic and duodenal ulcers. it is considered an important public health problem for both developed and developing world. this bacterium is classified as the class 1 carcinogen because it can cause cancer. | 2016 | 26833400 |
| cysteine mutational studies provide insight into a thiol-based redox switch mechanism of metal and dna binding in fura from anabaena sp. pcc 7120. | the ferric uptake regulator (fur) is the main transcriptional regulator of genes involved in iron homeostasis in most prokaryotes. fura from anabaena sp. pcc 7120 contains five cysteine residues, four of them arranged in two redox-active cxxc motifs. the protein needs not only metal but also reducing conditions to remain fully active in vitro. through a mutational study of the cysteine residues present in fura, we have investigated their involvement in metal and dna binding. | 2016 | 26414804 |
| magnetic resonance imaging differential diagnosis of brainstem lesions in children. | differential diagnosis of brainstem lesions, either isolated or in association with cerebellar and supra-tentorial lesions, can be challenging. knowledge of the structural organization is crucial for the differential diagnosis and establishment of prognosis of pathologies with involvement of the brainstem. familiarity with the location of the lesions in the brainstem is essential, especially in the pediatric population. magnetic resonance imaging (mri) is the most sensitive and specific imaging ... | 2016 | 26834941 |
| individual restriction of fine specificity variability in anti-gm1 igg antibodies associated with guillain-barré syndrome. | elevated titers of serum antibodies against gm1 ganglioside are associated with a variety of autoimmune neuropathies. much evidence indicates these autoantibodies play a primary role in the disease processes, but the mechanism for their appearance is unclear. we studied the fine specificity of anti-gm1 antibodies of the igg isotype present in sera from patients with guillain-barré syndrome (gbs), using thin-layer chromatogram-immunostaining of gm1, asialo-gm1 (ga1), gd1b and gm1-derivatives with ... | 2016 | 26818965 |
| diagnosing gastrointestinal illnesses using fecal headspace volatile organic compounds. | volatile organic compounds (vocs) emitted from stool are the components of the smell of stool representing the end products of microbial activity and metabolism that can be used to diagnose disease. despite the abundance of hydrogen, carbon dioxide, and methane that have already been identified in human flatus, the small portion of trace gases making up the vocs emitted from stool include organic acids, alcohols, esters, heterocyclic compounds, aldehydes, ketones, and alkanes, among others. thes ... | 2016 | 26819529 |
| an examination of co-infection in acute gastroenteritis and histo-blood group antigens leading to viral infection susceptibility. | the aim of the present study was to evaluate co-infection in the gastrointestinal tract in terms of viruses, bacteria and the abo blood group. we hypothesized that a combination of norovirus (nv) and bacteria in the gastrointestinal tract could affect the likelihood of an individual to contracting nv. histo-blood group antigens (hbgas) are considered to act as receptors that can lead to nv susceptibility. in addition to genetics, co-infection in the gastrointestinal tract may be associated with ... | 2016 | 26998270 |
| differential gene expression patterns in chicken cardiomyocytes during hydrogen peroxide-induced apoptosis. | hydrogen peroxide (h2o2) is both an exogenous and endogenous cytotoxic agent that can reliably induce apoptosis in numerous cell types for studies on apoptosis signaling pathways. however, little is known of these apoptotic processes in myocardial cells of chicken, a species prone to progressive heart failure. sequencing of mrna transcripts (rna-seq) allows for the identification of differentially expressed genes under various physiological and pathological conditions to elucidate the molecular ... | 2016 | 26808997 |
| novel α-l-fucosidases from a soil metagenome for production of fucosylated human milk oligosaccharides. | this paper describes the discovery of novel α-l-fucosidases and evaluation of their potential to catalyse the transglycosylation reaction leading to production of fucosylated human milk oligosaccharides. seven novel α-l-fucosidase-encoding genes were identified by functional screening of a soil-derived metagenome library and expressed in e. coli as recombinant 6xhis-tagged proteins. all seven fucosidases belong to glycosyl hydrolase family 29 (gh 29). six of the seven α-l-fucosidases were substr ... | 2016 | 26800369 |
| hib vaccines: past, present, and future perspectives. | haemophilus influenzae type b (hib) causes many severe diseases, including epiglottitis, pneumonia, sepsis, and meningitis. in developed countries, the annual incidence of meningitis caused by bacteria is approximately 5-10 cases per population of 100,000. the hib conjugate vaccine is considered protective and safe. adjuvants, molecules that can enhance and/or regulate the fundamental immunogenicity of an antigen, comprise a wide range of diverse compounds. while earlier developments of adjuvant ... | 2016 | 26904695 |
| evolutionary and functional relationships in the truncated hemoglobin family. | predicting function from sequence is an important goal in current biological research, and although, broad functional assignment is possible when a protein is assigned to a family, predicting functional specificity with accuracy is not straightforward. if function is provided by key structural properties and the relevant properties can be computed using the sequence as the starting point, it should in principle be possible to predict function in detail. the truncated hemoglobin family presents a ... | 2016 | 26788940 |
| within-host evolution of bacterial pathogens. | whole-genome sequencing has opened the way for investigating the dynamics and genomic evolution of bacterial pathogens during the colonization and infection of humans. the application of this technology to the longitudinal study of adaptation in an infected host--in particular, the evolution of drug resistance and host adaptation in patients who are chronically infected with opportunistic pathogens--has revealed remarkable patterns of convergent evolution, suggestive of an inherent repeatability ... | 2016 | 26806595 |
| molecular characterization and subtyping of blastocystis species in irritable bowel syndrome patients from north india. | blastocystis species has been extensively studied in recent few years to establish its pathogenecity. present study was designed to identify and examine the association of blastocystis sp. and its subtypes with irritable bowel syndrome (ibs).blastocystis sp. detected using wet-mount microscopy, trichrome staining, in-vitro culture and polymerase chain reaction (pcr) assay in a cohort of ibs patients (n = 150) and healthy controls (n = 100). isolates of blastocystis sp.were subtyped using sequenc ... | 2016 | 26784888 |
| phase variation of a type iig restriction-modification enzyme alters site-specific methylation patterns and gene expression in campylobacter jejuni strain nctc11168. | phase-variable restriction-modification systems are a feature of a diverse range of bacterial species. stochastic, reversible switches in expression of the methyltransferase produces variation in methylation of specific sequences. phase-variable methylation by both type i and type iii methyltransferases is associated with altered gene expression and phenotypic variation. one phase-variable gene of campylobacter jejuni encodes a homologue of an unusual type iig restriction-modification system in ... | 2016 | 26786317 |
| the influence of efflux pump inhibitors on the activity of non-antibiotic nsaids against gram-negative rods. | most patients with bacterial infections suffer from fever and various pains that require complex treatments with antibiotics, antipyretics, and analgaesics. the most common drugs used to relieve these symptoms are non-steroidal anti-inflammatory drugs (nsaids), which are not typically considered antibiotics. here, we investigate the effects of nsaids on bacterial susceptibility to antibiotics and the modulation of bacterial efflux pumps. | 2016 | 26771525 |
| alterations of the volatile metabolome in mouse models of alzheimer's disease. | in the present study, we tested whether the volatile metabolome was altered by mutations of the alzheimer's disease (ad)-implicated amyloid precursor protein gene (app) and comprehensively examined urinary volatiles that may potentially serve as candidate biomarkers of ad. establishing additional biomarkers in screening populations for ad will provide enhanced diagnostic specificity and will be critical in evaluating disease-modifying therapies. having strong evidence of gross changes in the vol ... | 2016 | 26762470 |
| association of ulcerative colitis with fut2 and fut3 polymorphisms in patients from southeast china. | dysbiosis of intestinal microbiota has been implicated in ulcerative colitis (uc). fucosyltransferase (fut) 2 and fut3 determine expression of histo-blood group antigens in the gut and may affect the intestinal microbiota. we investigated the association between fut2 and fut3 polymorphisms and uc in chinese patients. | 2016 | 26766790 |
| resistant starch alters the microbiota-gut brain axis: implications for dietary modulation of behavior. | the increasing recognition that the gut microbiota plays a central role in behavior and cognition suggests that the manipulation of microbial taxa through diet may provide a means by which behavior may be altered in a reproducible and consistent manner in order to achieve a beneficial outcome for the host. resistant starch continues to receive attention as a dietary intervention that can benefit the host through mechanisms that include altering the intestinal microbiota. given the interest in di ... | 2016 | 26745269 |
| rehabilitation of a patient with overlap of acute transverse myelitis and bickerstaff's brainstem encephalitis: a case report. | we report on one patient with bickerstaff's brainstem encephalitis (bbe) and associated flaccid weakness. counter to previous studies with bbe which indicate weakness due to guillain-barre syndrome, our patient's presentation of paraplegia following bbe is consistent with concomitant acute transverse myelitis. her findings of bbe largely resolved, although she remained with t6 american spinal injury association (asia) a paraplegia. motor functional impairment measure scores improved from 20 at a ... | 2016 | 28053734 |
| human milk components modulate toll-like receptor-mediated inflammation. | toll-like receptor (tlr) signaling is central to innate immunity. aberrant expression of tlrs is found in neonatal inflammatory diseases. several bioactive components of human milk modulate tlr expression and signaling pathways, including soluble toll-like receptors (stlrs), soluble cluster of differentiation (scd) 14, glycoproteins, small peptides, and oligosaccharides. some milk components, such as sialyl (α2,3) lactose and lacto-n-fucopentaose iii, are reported to increase tlr signaling; unde ... | 2016 | 26773018 |
| whole-genome sequencing analysis accurately predicts antimicrobial resistance phenotypes in campylobacter spp. | the objectives of this study were to identify antimicrobial resistance genotypes for campylobacter and to evaluate the correlation between resistance phenotypes and genotypes using in vitro antimicrobial susceptibility testing and whole-genome sequencing (wgs). a total of 114 campylobacter species isolates (82 c. coli and 32 c. jejuni) obtained from 2000 to 2013 from humans, retail meats, and cecal samples from food production animals in the united states as part of the national antimicrobial re ... | 2016 | 26519386 |
| removal of fecal indicators, pathogenic bacteria, adenovirus, cryptosporidium and giardia (oo)cysts in waste stabilization ponds in northern and eastern australia. | maturation ponds are used in rural and regional areas in australia to remove the microbial loads of sewage wastewater, however, they have not been studied intensively until present. using a combination of culture-based methods and quantitative real-time pcr, we assessed microbial removal rates in maturation ponds at four waste stabilization ponds (wsp) with (n = 1) and without (n = 3) baffles in rural and remote communities in australia. concentrations of total coliforms, e. coli, enterococci, c ... | 2016 | 26729150 |
| acute motor axonal neuropathy (aman) with motor conduction blocks in childhood; case report. | objective acute motor axonal neuropathy (aman), characterized with decreased compound muscle action potentials (cmap) and absence of demyelinating findings in electrophysiological studies, is a subtype of guillain-barre syndrome (gbs). a 4 yr-old male patient presented with ascending weakness, dysarthria and dysphagia to istanbul dr. lütfi kırdar kartal training and research hospital neurology outpatient for three days to in 2012. dysphonia, restricted eye movements, flaccid tetraplegia and aref ... | 2016 | 27057191 |
| multidrug resistant shigella flexneri infection simulating intestinal intussusception. | shigella enteritis remains an important cause of mortality and morbidity in all age groups, in developing as well as developed countries. owing to the emerging resistance to multiple antibiotics among shigella spp., it has been recognized as a major global public health concern and warrants constant monitoring of its resistance pattern. we report a case of segmental ileitis caused by non.-esbl producing multidrug resistant shigella flexneri in an infant clinically mimicking intussusception, whic ... | 2016 | 27013815 |
| sialic acids and autoimmune disease. | an important underlying mechanism that contributes to autoimmunity is the loss of inhibitory signaling in the immune system. sialic acid-recognizing ig superfamily lectins or siglecs are a family of cell surface proteins largely expressed in hematopoietic cells. the majority of siglecs are inhibitory receptors expressed in immune cells that bind to sialic acid-containing ligands and recruit sh2-domain-containing tyrosine phosphatases to their cytoplasmic tails. they deliver inhibitory signals th ... | 2016 | 26683151 |
| onset of ulcerative colitis during a low-carbohydrate weight-loss diet and treatment with a plant-based diet: a case report. | overweight and obesity are global health concerns. various effective weight-loss diets have been developed, including the atkins diet. the atkins diet is known as an extreme low-carbohydrate diet. this diet reduces body weight and has gained widespread popularity. however, the metabolite profiles of such a diet have been shown to be detrimental to colonic health. therefore, a concern for the long-term health effects of this diet exists. we encountered a case in which ulcerative colitis developed ... | 2016 | 26824967 |
| a comparison between plasmapheresis and intravenous immunoglobulin in children with guillain-barré syndrome in upper egypt. | the aim of our study is to assess the clinico-electrophysiological profile of children with guillain-barré syndrome (gbs) in upper egypt and to compare the efficacy of plasmapheresis versus other treatment modalities. | 2016 | 26788127 |
| a gastrointestinal anti-infectious biotherapeutic agent: the heat-treated lactobacillus lb. | experimental in vitro and in vivo studies support the hypothesis that heat-treated, lyophilized lactobacillus acidophilus lb cells and concentrated, neutralized spent culture medium conserve the variety of pharmacological, antimicrobial activities of the live probiotic strain against several infectious agents involved in well-established acute and persistent watery diarrhoea and gastritis. heat-treated cells and heat-stable secreted molecules trigger multiple strain-specific activities explainin ... | 2016 | 26770268 |
| prevalence and risk factors for campylobacter spp., salmonella spp., coxiella burnetii, and newcastle disease virus in feral pigeons (columba livia) in public areas of montreal, canada. | feral pigeons (columbia livia) can harbor a range of zoonotic pathogens. a transversal study was undertaken to estimate the prevalence of feral pigeons infected by various pathogens in public areas in montreal, quebec. cloacal swabs from captured birds were cultured for salmonella spp. and campylobacter spp. and tested by real-time polymerase chain reaction (rt-pcr) for the detection of coxiella burnetii. an oropharyngeal swab was also submitted to real-time reverse-transcription polymerase chai ... | 2016 | 26733736 |
| reducing the risk of pet-associated zoonotic infections. | 2015 | 25897046 | |
| structures of bacteroides fragilis uridine 5'-diphosphate-n-acetylglucosamine (udp-glcnac) acyltransferase (bflpxa). | uridine 5'-diphosphate-n-acetylglucosamine (udp-glcnac) acyltransferase (lpxa) catalyzes a reversible reaction for adding an o-acyl group to the glcnac in udp-glcnac in the first step of lipid a biosynthesis. lipid a constitutes a major component of lipopolysaccharides, also referred to as endotoxins, which form the outer monolayer of the outer membrane of gram-negative bacteria. ligand-free and udp-glcnac-bound crystal structures of lpxa from bacteroides fragilis nctc 9343, the most common path ... | 2015 | 25945572 |
| detection of mycobacteria, mycobacterium avium subspecies, and mycobacterium tuberculosis complex by a novel tetraplex real-time pcr assay. | mycobacterium tuberculosis complex, mycobacterium avium, and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical and veterinary importance. considering the growing epidemiologic significance of wildlife-livestock-human interrelation, developing rapid detection tools of high specificity and sensitivity is vital to assess their presence and accelerate the process of diagnosing mycobacteriosis. here we describe the development and evaluation of a novel t ... | 2015 | 25588660 |
| development of a new real-time pcr system for simultaneous detection of bacteria and fungi in pathological samples. | a novel system for simultaneous detection of pathogenic bacteria and fungi in pathological samples was developed using a real-time polymerase chain reaction (pcr) system. this system, designated the "multi-microbial real-time pcr", has the potential to simultaneously detect 68 bacterial and 9 fungal species in a 96-well plate format. all probe-primer sets were designed to produce amplicons smaller than 210 bp using formalin-fixed paraffin-embedded samples as input. the specificity and sensitivit ... | 2015 | 26823918 |
| hypothetical protein ct398 (cdsz) interacts with σ(54) (rpon)-holoenzyme and the type iii secretion export apparatus in chlamydia trachomatis. | a significant challenge to bacteriology is the relatively large proportion of proteins that lack sufficient sequence similarity to support functional annotation (i.e. hypothetical proteins). the aim of this study was to apply protein structural homology to gain insights into a candidate protein of unknown function (ct398) within the medically important, obligate intracellular bacterium chlamydia trachomatis. c. trachomatis is a major human pathogen responsible for numerous infections throughout ... | 2015 | 26173998 |
| mechanisms of drug resistance: quinolone resistance. | quinolone antimicrobials are synthetic and widely used in clinical medicine. resistance emerged with clinical use and became common in some bacterial pathogens. mechanisms of resistance include two categories of mutation and acquisition of resistance-conferring genes. resistance mutations in one or both of the two drug target enzymes, dna gyrase and dna topoisomerase iv, are commonly in a localized domain of the gyra and pare subunits of the respective enzymes and reduce drug binding to the enzy ... | 2015 | 26190223 |
| rho gtpases as pathogen targets: focus on curable sexually transmitted infections. | pathogens have evolved highly specialized mechanisms to infect hosts. several microorganisms modulate the eukaryotic cell surface to facilitate their engulfment. once internalized, they hijack the molecular machinery of the infected cell for their own benefit. at different stages of phagocytosis, particularly during invasion, certain pathogens manipulate pathways governed by small gtpases. in this review, we focus on the role of rho proteins on curable, sexually transmitted infections caused by ... | 2015 | 26023809 |
| bridging topological and functional information in protein interaction networks by short loops profiling. | protein-protein interaction networks (ppins) have been employed to identify potential novel interconnections between proteins as well as crucial cellular functions. in this study we identify fundamental principles of ppin topologies by analysing network motifs of short loops, which are small cyclic interactions of between 3 and 6 proteins. we compared 30 ppins with corresponding randomised null models and examined the occurrence of common biological functions in loops extracted from a cross-vali ... | 2015 | 25703051 |
| network representations of immune system complexity. | the mammalian immune system is a dynamic multiscale system composed of a hierarchically organized set of molecular, cellular, and organismal networks that act in concert to promote effective host defense. these networks range from those involving gene regulatory and protein-protein interactions underlying intracellular signaling pathways and single-cell responses to increasingly complex networks of in vivo cellular interaction, positioning, and migration that determine the overall immune respons ... | 2015 | 25625853 |
| the altered schaedler flora: continued applications of a defined murine microbial community. | the gastrointestinal (gi) microbiota forms a mutualistic relationship with the host through complex and dynamic interactions. because of the complexity and interindividual variation of the gi microbiota, investigating how members of the microbiota interact with each other, as well as with the host, is daunting. the altered schaedler flora (asf) is a model community of eight microorganisms that was developed by r.p. orcutt and has been in use since the late 1970s. the eight microorganisms composi ... | 2015 | 26323627 |
| differential distribution of type ii crispr-cas systems in agricultural and nonagricultural campylobacter coli and campylobacter jejuni isolates correlates with lack of shared environments. | crispr (clustered regularly interspaced palindromic repeats)-cas (crispr-associated) systems are sequence-specific adaptive defenses against phages and plasmids which are widespread in prokaryotes. here we have studied whether phylogenetic relatedness or sharing of environmental niches affects the distribution and dissemination of type ii crispr-cas systems, first in 132 bacterial genomes from 15 phylogenetic classes, ranging from proteobacteria to actinobacteria. there was clustering of distinc ... | 2015 | 26338188 |
| effects of hangeshashinto on growth of oral microorganisms. | oral mucositis (om) in cancer patients induced by chemotherapy or radiotherapy has a significant impact on quality of life, and causes considerable morbidity. oral microorganisms are likely to intensify the inflammatory process and aggravate the formation of ulcers. hangeshashinto (hst), a japanese kampo medicine, has been reported to be effective when used as a gargle for the treatment of om. to clarify the effects of hst on oral microorganisms, we assessed its antimicrobial activity against 27 ... | 2015 | 26170876 |
| traditional uses, phytochemistry, and pharmacology of olea europaea (olive). | aim of the review. to grasp the fragmented information available on the botany, traditional uses, phytochemistry, pharmacology, and toxicology of olea europaea to explore its therapeutic potential and future research opportunities. material and methods. all the available information on o. europaea was collected via electronic search (using pubmed, scirus, google scholar, and web of science) and a library search. results. ethnomedical uses of o. europaea are recorded throughout the world where it ... | 2015 | 25802541 |
| mouse genome engineering via crispr-cas9 for study of immune function. | clustered regularly interspaced palindromic repeats (crispr)-associated (cas9) technology has proven a formidable addition to our armory of approaches for genomic editing. derived from pathways in archaea and bacteria that mediate the resistance to exogenous genomic material, the crispr-cas9 system utilizes a short single guide rna (sgrna) to direct the endonuclease cas9 to virtually anywhere in the genome. upon targeting, cas9 generates dna double-strand breaks (dsbs) and facilitates the repair ... | 2015 | 25607456 |
| lengths of orthologous prokaryotic proteins are affected by evolutionary factors. | proteins of the same functional family (for example, kinases) may have significantly different lengths. it is an open question whether such variation in length is random or it appears as a response to some unknown evolutionary driving factors. the main purpose of this paper is to demonstrate existence of factors affecting prokaryotic gene lengths. we believe that the ranking of genomes according to lengths of their genes, followed by the calculation of coefficients of association between genome ... | 2015 | 26114113 |
| outer membrane vesicles as platform vaccine technology. | outer membrane vesicles (omvs) are released spontaneously during growth by many gram-negative bacteria. they present a range of surface antigens in a native conformation and have natural properties like immunogenicity, self-adjuvation and uptake by immune cells which make them attractive for application as vaccines against pathogenic bacteria. in particular with neisseria meningitidis, they have been investigated extensively and an omv-containing meningococcal vaccine has recently been approved ... | 2015 | 26912077 |
| membrane topology and biochemical characterization of the escherichia coli baca undecaprenyl-pyrophosphate phosphatase. | several integral membrane proteins exhibiting undecaprenyl-pyrophosphate (c55-pp) phosphatase activity were previously identified in escherichia coli that belonged to two distinct protein families: the baca protein, which accounts for 75% of the c55-pp phosphatase activity detected in e. coli cell membranes, and three members of the pap2 phosphatidic acid phosphatase family, namely pgpb, ybjg and lpxt. this dephosphorylation step is required to provide the c55-p carrier lipid which plays a centr ... | 2015 | 26560897 |
| pathogenesis of myeloproliferative neoplasms: role and mechanisms of chronic inflammation. | myeloproliferative neoplasms (mpns) are a heterogeneous group of clonal diseases characterized by the excessive and chronic production of mature cells from one or several of the myeloid lineages. recent advances in the biology of mpns have greatly facilitated their molecular diagnosis since most patients present with mutation(s) in the jak2, mpl, or calr genes. yet the roles played by these mutations in the pathogenesis and main complications of the different subtypes of mpns are not fully eluci ... | 2015 | 26538820 |
| development of real-time pcr assays for the detection of moraxella macacae associated with bloody nose syndrome in rhesus (macaca mulatta) and cynomolgus (macaca fascicularis) macaques. | moraxella macacae is a recently described bacterial pathogen that causes epistaxis or so-called bloody nose syndrome in captive macaques. the aim of this study was to develop specific molecular diagnostic assays for m. macacae and to determine their performance characteristics. | 2015 | 26365904 |
| opposite and coordinated rotation of amphitrichous flagella governs oriented swimming and reversals in a magnetotactic spirillum. | current knowledge regarding the mechanism that governs flagellar motor rotation in response to environmental stimuli stems mainly from the study of monotrichous and peritrichous bacteria. little is known about how two polar flagella, one at each cell pole of the so-called amphitrichous bacterium, are coordinated to steer the swimming. here we fluorescently labeled the flagella of magnetospirillum magneticum amb-1 cells and took advantage of the magnetically controllable swimming of this bacteriu ... | 2015 | 26240070 |
| lessons in fundamental mechanisms and diverse adaptations from the 2015 bacterial locomotion and signal transduction meeting. | in response to rapid changes in their environment, bacteria control a number of processes, including motility, cell division, biofilm formation, and virulence. research presented in january 2015 at the biennial bacterial locomotion and signal transduction (blast) meeting in tucson, az, illustrates the elegant complexity of the nanoarrays, nanomachines, and networks of interacting proteins that mediate such processes. studies employing an array of biophysical, genetic, cell biology, and mathemati ... | 2015 | 26195592 |
| ileal mucosa-associated lymphoid tissue lymphoma presenting with small bowel obstruction: a case report. | extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (malt lymphoma) of the gastrointestinal tract commonly involves the stomach in the setting of concurrent helicobacter pylori (h. pylori) infection. primary ileal malt lymphoma is rare, and has not been associated with a specific infectious disease. we report a case of a 58-year-old man who presented to the emergency department with constipation and abdominal distension, and signs of an obstructing mass on computed tomography ... | 2015 | 26178711 |
| acylation of biomolecules in prokaryotes: a widespread strategy for the control of biological function and metabolic stress. | acylation of biomolecules (e.g., proteins and small molecules) is a process that occurs in cells of all domains of life and has emerged as a critical mechanism for the control of many aspects of cellular physiology, including chromatin maintenance, transcriptional regulation, primary metabolism, cell structure, and likely other cellular processes. although this review focuses on the use of acetyl moieties to modify a protein or small molecule, it is clear that cells can use many weak organic aci ... | 2015 | 26179745 |
| investigating reports of complex regional pain syndrome: an analysis of hpv-16/18-adjuvanted vaccine post-licensure data. | complex regional pain syndrome (crps) is a chronic pain disorder that typically follows trauma or surgery. suspected crps reported after vaccination with human papillomavirus (hpv) vaccines led to temporary suspension of proactive recommendation of hpv vaccination in japan. we investigated the potential crps signal in relation to hpv-16/18-adjuvanted vaccine (cervarix®) by database review of crps cases with independent expert confirmation; a disproportionality analysis and analyses of temporalit ... | 2015 | 26501109 |
| mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator. | ferric uptake regulator (fur) plays a key role in the iron homeostasis of prokaryotes, such as bacterial pathogens, but the molecular mechanisms and structural basis of fur-dna binding remain incompletely understood. here, we report high-resolution structures of magnetospirillum gryphiswaldense msr-1 fur in four different states: apo-fur, holo-fur, the fur-feoab1 operator complex and the fur-pseudomonas aeruginosa fur box complex. apo-fur is a transition metal ion-independent dimer whose binding ... | 2015 | 26134419 |
| architecture and roles of periplasmic adaptor proteins in tripartite efflux assemblies. | recent years have seen major advances in the structural understanding of the different components of tripartite efflux assemblies, which encompass the multidrug efflux (mdr) pumps and type i secretion systems. the majority of these investigations have focused on the role played by the inner membrane transporters and the outer membrane factor (omf), leaving the third component of the system - the periplasmic adaptor proteins (paps) - relatively understudied. here we review the current state of knowle ... | 2015 | 26074901 |
| extensive infectious myelitis post bariatric surgery. | inflammatory myelopathy is an inflammatory neurological disorder of the spinal cord (myelopathy). it occurs in 1 (severe) to 8 (mild) cases/million per year. it is often referred to in the literature as "transverse myelitis" or "acute transverse myelitis". myelopathy and by extension myelitis, can present as pyramidal (motor), sensory, and/or autonomic dysfunction to varying degrees. symptoms typically develop over hours to days and worsen over days to weeks. sensory symptoms usually present as ... | 2015 | 25879204 |
| presence of zoonotic agents in engorged ticks and hedgehog faeces from erinaceus europaeus in (sub) urban areas. | european hedgehogs (erinaceus europaeus) are hosts for ixodes hexagonus and i. ricinus ticks, which are vectors for zoonotic microorganisms. in addition, hedgehogs may carry several enteric zoonoses as well. it is unclear to what extent a presence of pathogens in hedgehogs poses a risk to public health, as information on the presence of zoonotic agents in hedgehogs in urban areas is relatively scarce. | 2015 | 25885888 |
| genome-wide dna methylation analysis of haloferax volcanii h26 and identification of dna methyltransferase related pd-(d/e)xk nuclease family protein hvo_a0006. | restriction-modification (rm) systems have evolved to protect the cell from invading dnas and are composed of two enzymes: a dna methyltransferase and a restriction endonuclease. although rm systems are present in both archaeal and bacterial genomes, dna methylation in archaea has not been well defined. in order to characterize the function of rm systems in archaeal species, we have made use of the model haloarchaeon haloferax volcanii. a genomic dna methylation analysis of h. volcanii strain h2 ... | 2015 | 25904898 |
| molecular pathogenesis of malt lymphoma. | approximately 8% of all non-hodgkin lymphomas are extranodal marginal zone b cell lymphoma of mucosa associated lymphoid tissue (malt), also known as malt lymphoma, which was first described in 1983 by isaacson and wright. malt lymphomas arise at a wide range of different extranodal sites, with the highest frequency in the stomach, followed by lung, ocular adnexa, and thyroid, and with a low percentage in the small intestine. interestingly, at least 3 different, apparently site-specific, chromos ... | 2015 | 25922601 |
| regulation of bacterial virulence by csr (rsm) systems. | most bacterial pathogens have the remarkable ability to flourish in the external environment and in specialized host niches. this ability requires their metabolism, physiology, and virulence factors to be responsive to changes in their surroundings. it is no surprise that the underlying genetic circuitry that supports this adaptability is multilayered and exceedingly complex. studies over the past 2 decades have established that the csra/rsma proteins, global regulators of posttranscriptional ge ... | 2015 | 25833324 |
| the challenge of efflux-mediated antibiotic resistance in gram-negative bacteria. | the global emergence of multidrug-resistant gram-negative bacteria is a growing threat to antibiotic therapy. the chromosomally encoded drug efflux mechanisms that are ubiquitous in these bacteria greatly contribute to antibiotic resistance and present a major challenge for antibiotic development. multidrug pumps, particularly those represented by the clinically relevant acrab-tolc and mex pumps of the resistance-nodulation-division (rnd) superfamily, not only mediate intrinsic and acquired mult ... | 2015 | 25788514 |
| a new player at the flagellar motor: flil controls both motor output and bias. | the bacterial flagellum is driven by a bidirectional rotary motor, which propels bacteria to swim through liquids or swarm over surfaces. while the functions of the major structural and regulatory components of the flagellum are known, the function of the well-conserved flil protein is not. in salmonella and escherichia coli, the absence of flil leads to a small defect in swimming but complete elimination of swarming. here, we tracked single motors of these bacteria and found that absence of fli ... | 2015 | 25714720 |
| proteomic profiling of the outer membrane fraction of the obligate intracellular bacterial pathogen ehrlichia ruminantium. | the outer membrane proteins (omps) of gram-negative bacteria play a crucial role in virulence and pathogenesis. identification of these proteins represents an important goal for bacterial proteomics, because it aids in vaccine development. here, we have developed such an approach for ehrlichia ruminantium, the obligate intracellular bacterium that causes heartwater. a preliminary whole proteome analysis of elementary bodies, the extracellular infectious form of the bacterium, had been performed ... | 2015 | 25710494 |
| exploring the existence of lipid rafts in bacteria. | an interesting concept in the organization of cellular membranes is the proposed existence of lipid rafts. membranes of eukaryotic cells organize signal transduction proteins into membrane rafts or lipid rafts that are enriched in particular lipids such as cholesterol and are important for the correct functionality of diverse cellular processes. the assembly of lipid rafts in eukaryotes has been considered a fundamental step during the evolution of cellular complexity, suggesting that bacteria a ... | 2015 | 25652542 |
| extranodal marginal zone lymphoma of the lung: evolution from an underlying reactive lymphoproliferative disorder. | extranodal marginal zone lymphoma (enmzl) of mucosa-associated lymphoid tissue (malt) is a problematic and sometimes controversial diagnosis. while commonly seen in the stomach in the setting of chronic helicobacter pylori infection, other extranodal sites, such as the lung, may also present with disease. enmzl is clinically and morphologically heterogeneous; however, regardless of presentation, the etiology lies in the accumulation of lymphoid tissue in non-traditional sites. this phenomenon is ... | 2015 | 26457231 |
| a case of a laryngeal malt lymphoma in a patient with a history of gastric malt. | we are reporting a case of a 62-year-old african american woman with a history of gastric malt lymphoma successfully treated with radiation who presented with a laryngeal malt lymphoma 4 years after her original diagnosis. she received definitive radiation with a complete response. the case presented is unique for the rare presentation of a malt lymphoma in the larynx, especially in light of the patient's previously treated gastric malt lymphoma years ago. | 2015 | 25664189 |
| current and past strategies for bacterial culture in clinical microbiology. | a pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. the first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. the rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. th ... | 2015 | 25567228 |
| evolution of the selenoproteome in helicobacter pylori and epsilonproteobacteria. | by competing for the acquisition of essential nutrients, helicobacter pylori has the unique ability to persist in the human stomach, also causing nutritional insufficiencies in the host. although the h. pylori genome apparently encodes selenocysteine synthase (sela, hp1513), a key pyridoxal phosphate (plp)-dependent enzyme for the incorporation of selenium into bacterial proteins, nothing is known about the use of this essential element in protein synthesis by this pathogen. we analyzed the evol ... | 2015 | 26342139 |
| global epidemiology of campylobacter infection. | campylobacter jejuni infection is one of the most widespread infectious diseases of the last century. the incidence and prevalence of campylobacteriosis have increased in both developed and developing countries over the last 10 years. the dramatic increase in north america, europe, and australia is alarming, and data from parts of africa, asia, and the middle east indicate that campylobacteriosis is endemic in these areas, especially in children. in addition to c. jejuni, there is increasing rec ... | 2015 | 26062576 |
| targeting surface-layer proteins with single-domain antibodies: a potential therapeutic approach against clostridium difficile-associated disease. | clostridium difficile is a leading cause of death from gastrointestinal infections in north america. antibiotic therapy is effective, but the high incidence of relapse and the rise in hypervirulent strains warrant the search for novel treatments. surface layer proteins (slps) cover the entire c. difficile bacterial surface, are composed of high-molecular-weight (hmw) and low-molecular-weight (lmw) subunits, and mediate adherence to host cells. passive and active immunization against slps has enh ... | 2015 | 25936376 |
| retraction for dinant et al., aortic homograft endocarditis caused by campylobacter jejuni. | 2015 | 25617446 | |
| the polar and lateral flagella from plesiomonas shigelloides are glycosylated with legionaminic acid. | plesiomonas shigelloides is the unique member of the enterobacteriaceae family able to produce polar flagella when grow in liquid medium and lateral flagella when grown in solid or semisolid media. in this study on p. shigelloides 302-73 strain, we found two different gene clusters, one exclusively for the lateral flagella biosynthesis and the other one containing the biosynthetic polar flagella genes with additional putative glycosylation genes. p. shigelloides is the first enterobacteriaceae w ... | 2015 | 26167161 |
| bacterial flagella: twist and stick, or dodge across the kingdoms. | the flagellum organelle is an intricate multiprotein assembly best known for its rotational propulsion of bacteria. however, recent studies have expanded our knowledge of other functions in pathogenic contexts, particularly adherence and immune modulation, e.g., for salmonella enterica, campylobacter jejuni, pseudomonas aeruginosa, and escherichia coli. flagella-mediated adherence is important in host colonisation for several plant and animal pathogens, but the specific interactions that promote ... | 2015 | 25590430 |
| survival in water of campylobacter jejuni strains isolated from the slaughterhouse. | campylobacter jejuni cause gastroenteritis in humans. the main transmission vector is the consumption or handling of contaminated chicken meat, since chicken can be colonized asymptomatically by c. jejuni. however, water has been implicated as the transmission vector in a few outbreaks. one possibility is the contamination of water effluent by c. jejuni originating from chicken farm. the ability of c. jejuni to be transmitted by water would be closely associated to its ability to survive in wate ... | 2015 | 26702388 |
| smrt sequencing of the campylobacter coli bfr-ca-9557 genome sequence reveals unique methylation motifs. | campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. in contrast to campylobacter jejuni, about 5 % of campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by dpni cutting specifically 5'-g(m)atc-3' motifs. this indicates significant differences in dna methylation between both microbial species. the goal of the study was to analyze the methylome of a c. coli strain susceptible to dpni digestion, to identify its meth ... | 2015 | 26689587 |
| molecular identification of campylobacter jejuni and coli from chicken, calves and dogs to determine its potential threat on human being. | campylobacter is an emerging zoonotic pathogen and one of the leading cause of foodborne infection worldwide and it has been isolated from a variety of animal species. the aim of this study was to identify campylobacter jejuni and campylobacter coli from dogs, calves, and poultry using polymerase chain reaction (pcr). | 2015 | 27047055 |