Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| campylobacter jejuni and campylobacter coli diarrhoea in rural and urban populations in yugoslavia. | during a 4-month period during the summer of 1985, campylobacters were isolated from 338 (16.3%) of 2080 patients with acute diarrhoea attending the university hospital of infectious diseases in zagreb. of these isolates 220 (64.1%) were campylobacter jejuni and 118 (34.9%) were c. coli. the patients were drawn from three residential zones in and around zagreb: inner city, peripheral city and rural. incidences of campylobacter diarrhoea ranged from 71 per 100,000 per year in inner city residents ... | 1989 | 2917618 |
| pancreatitis associated with campylobacter jejuni infection: diagnosis by ultrasonography. | 1989 | 2499366 | |
| experimental production of proliferative ileitis in syrian hamsters (mesocricetus auratus) by using an ileal homogenate free of campylobacter jejuni. | the role of campylobacter jejuni in the pathogenesis of proliferative ileitis of syrian hamsters (mesocricetus auratus) has been uncertain. c. jejuni has been implicated as the etiologic agent on the basis of the campylobacter-type morphology of the intracellular organism and the repeated microbiologic isolation of c. jejuni from hamsters with proliferative ileitis. the inability to reproduce the disease with pure culture inocula, coupled with immunohistochemical studies, however, has suggested ... | 1989 | 2462537 |
| effects of sample holding time, temperature, and atmosphere on the isolation of campylobacter jejuni from dogs. | stool specimens were collected from 39 dogs, inoculated onto campylobacter blood agar plates, and divided into four subsamples. subsamples were held at 4 and 25 degrees c in room air and in a microaerobic environment and were reinoculated at 1, 2, 3, 4, 6, and 8 h. c. jejuni survived at least 3 h when it was held at 4 degrees c, but less than 2 h when it was held at 25 degrees c. the holding atmosphere was not associated with a difference in isolation rates. | 1989 | 2754014 |
| waterborne outbreak of campylobacter enteritis after outdoors infantry drill in utti, finland. | diarrhoea, abdominal pain, malaise and fever affected 75 of the 88 conscript soldiers in utti, finland after an outdoors infantry drill. campylobacter jejuni, heat-stable serotype 3/43/59, was isolated from 37 out of 63 men investigated. a clear serological response was evident in the risk group and negligible in the control group. the entire population at risk was interviewed. the outbreak was associated with the consumption of untreated surface water. c. jejuni, heat-stable serotype 3/43/59, w ... | 1989 | 2776848 |
| interaction of campylobacter species with antibody, complement and phagocytes. | the opsonisation of four different campylobacter species for human neutrophils was studied using a chemiluminescence system and electron microscopy. opsonisation of campylobacter fetus, campylobacter coli, and campylobacter jejuni was mediated by antibody and enhanced by complement. antibody was not, however, required for the phagocytosis of campylobacter pylori because it activates the classical pathway of complement directly. this unusual property may be important in the pathogenesis of c pylo ... | 1989 | 2788111 |
| in-vitro evaluation of antibiotic combinations against campylobacter fetus. | we evaluated the in-vitro activity of 16 antibiotics against 22 clinical isolates of campylobacter fetus. the interaction of these antibiotics was also tested by chequer-board titration and time-kill assay. eight antibiotic combinations were evaluated. antagonism was not found with any of the combinations tested. the highest synergistic effect was obtained with a combination of ampicillin and cefazolin or ampicillin and gentamicin. | 1989 | 2793642 |
| in vitro evaluation of antibiotics for treatment of meningitis caused by campylobacter fetus subspecies fetus. | 1989 | 2797963 | |
| enterotoxin production and serogroups of campylobacter jejuni and campylobacter coli from patients with diarrhea and from healthy laying hens. | enterotoxin production, a possible virulence factor, was determined in campylobacter jejuni and campylobacter coli by two different techniques, the cho cell test and the gm1 enzyme-linked immunosorbent assay. the frequency of enterotoxigenic campylobacter strains was 32% in strains from both humans with acute enteritis and healthy laying hens, as measured by the cho cell test. the cho cell test was significantly more sensitive than the gm1 enzyme-linked immunosorbent assay in the detection of en ... | 1989 | 2754001 |
| response of the chick embryo to live and heat-killed campylobacter jejuni injected into the yolk sac. | graded doses of live and heat-killed cells of campylobacter jejuni were injected into the yolk-sac of 5-day-old chick embryos, and the 50% lethal dose (ld50) was determined 7 days later. a strain dependent virulence was seen. in the diluted series of cultures the ld50 values for live campylobacter ranged from 10(6) c.f.u. beyond the last dilution showing growth, that is to less than one organism per embryo. when the 22 strains were tested as heat-killed cells, the chick embryo ld50 values retain ... | 1989 | 2606163 |
| [infectious diarrhea diseases]. | gastroenteritis may be due to many agents, infectious or non infections. most cases of infections diarrhea are selflimited--e.g. food born intoxications with 1 to 2 days duration or infections with enteropathogenic e. coli, salmonella spp., vibrio parahaemolyticus or rota virus. even the majority of shigellosis, salmonella-gastroenteritis and infections with campylobacter fetus cease without treatment within 4 days. fever and diarrhea persisting for more than 4 days justify the use of antibiotic ... | 1989 | 2662618 |
| immune response to campylobacter jejuni and campylobacter coli in a cohort of children from birth to 2 years of age. | a cohort of 111 children from bangui, central african republic, was surveyed for enteric campylobacter infections from birth to the age of 2 years; stools were examined biweekly in these children until 6 months of age and at least four times per year thereafter until 2 years of age and after each diarrheal episode. blood samples were obtained at birth and at 3, 6, 9, 12, 18, and 24 months of age. antibodies against glycine-extracted membrane antigens, purified flagella, and cholera toxin (ct) we ... | 1989 | 2744860 |
| hexagonal surface layer of campylobacter fetus isolated from humans. | electron microscopic studies of the surface structure of campylobacter fetus by the freeze-etching method showed two different types of s layer. one was in a hexagonal array, and the other was in a tetragonal array. a high-passage-number strain lost its s layer during cultivation on culture media but regained it after a single animal passage. | 1989 | 2744862 |
| identification of campylobacter jejuni and c. coli by gel electrophoresis of the outer membrane proteins. | analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate campylobacter jejuni (16 strains) from campylobacter coli (10 strains). this observation was confirmed by the study of dna homology obtained by a quantitative filter hybridization method. the hippurate hydrolysis test gave a poor correlation with the results of differentiation obtained by dna homology studies and outer membrane protein profile. | 1989 | 2745680 |
| campylobacter fetus cholangitis and bacteremia. | 1989 | 2804850 | |
| [sepsis caused by campylobacter fetus spp. fetus]. | 1989 | 2491527 | |
| flagellin expression in campylobacter jejuni is regulated at the transcriptional level. | campylobacter jejuni 81116 is able to switch flagellum formation on and off. to study the expression of flagellin, the main component of flagella, an expression library of c. jejuni dna was constructed in lambda gt11. screening of this library with a flagellin-specific antiserum resulted in a clone producing a beta-galactosidase-flagellin fusion protein; it contained a dna insert of 850 base pairs and coded for about 15 kilodaltons of the flagellin, corresponding to 410 base pairs of the flagell ... | 1989 | 2466792 |
| [a case report of campylobacter fetus subspecies fetus bacteremia]. | an unusual case of campylobacter fetus subspecies fetus bacteremia was presented. a twenty four year old male was admitted to our hospital due to abdominal pain, general malaise, diarrhea, high fever, and hemoptysis. he was alcoholic and fond of eating raw liver. he had a history of partial gastrectomy and disturbance of pancreatic function. he showed pulmonary empyema, pleuritis, thrombophlebitis of lower legs, jaundice, hepatomegaly, diarrhea, pneumothorax, and low t3 low t4 syndrome. c. fetus ... | 1989 | 2695582 |
| serotyping studies of campylobacter from naturally colonized chickens. | campylobacter jejuni/coli strains from 164 chickens were serotyped by the methods previously described by penner et al. and lior et al. the chickens were sampled during breeding from hatching to the age of 42 weeks. the birds were housed, in two separate groups, under different environmental conditions, (for comparison of the effect of hygienic precautions on the transmission of the bacteria during breeding). in the group where the hygienic conditions could be controlled to a greater extent, the ... | 1989 | 2703018 |
| comparison of different vaccines and induced immune response against campylobacter jejuni colonization in the infant mouse. | the degree of protection conferred by vaccinated dams on infant mice against colonization by campylobacter jejuni depended on the bacterial strain, preparation, and route of administration of the vaccine. in some instances of homologous protection, serum bactericidal titres correlated well with protection. however, boiled c. jejuni vaccine, which was non-protective, also elicited a strong bactericidal antibody response. conversely, bactericidal activity could not be demonstrated against strains ... | 1989 | 2703020 |
| population genetics of human and animal enteric campylobacter strains. | a total of 125 strains of campylobacter jejuni and campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. the nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. the strains were grouped into 64 electrophoretic types (ets) forming two principal clusters; the first ... | 1989 | 2707852 |
| experimental campylobacter jejuni infection in macaca nemestrina. | experimental infection of four specific-pathogen-free macaca nemestrina monkeys (aged 3.5 and 4.5 months) with campylobacter jejuni 81-176 caused acute diarrheal illness, characterized by fluid diarrhea, bloody stools, and fecal leukocytes, which lasted for approximately 7 to 11 days. histologic examination of intestinal biopsies showed acute colitis characterized by infiltration of the mucosa with neutrophils and lymphocytes, and cryptitis. there were no histologic changes in the small intestin ... | 1989 | 2707853 |
| [the most frequent infectious causes of abortion in sheep in north bavaria with special reference to chlamydia and salmonella infections]. | between 1980 and 1987 1153 ovine fetuses and placentas were examined after abortion. in 68.5% of the cases a cause of abortion could be diagnosed as follows: 43.5% chlamydia psittaci var. ovis, 10.7% salmonella abortus ovis, 3.7% coxiella burnetii, 3.3% listeria monocytogenes and 0.4% campylobacter fetus intestinalis. hemolyzing escherichia coli and streptococci, yersinia pseudotuberculosis, salmonella typhimurium, salmonella enteritidis, corynebacterium pyogenes and other facultative pathogens ... | 1989 | 2503908 |
| antigenic and restriction enzyme analysis of isolates of campylobacter fetus subsp venerealis recovered from persistently infected cattle. | thirty-two isolates of campylobacter fetus subsp venerealis were obtained from 1 bull and 4 heifers with experimentally induced infection. when whole-cell antigens of isolates were cross titrated with antisera to the infecting strain, isolates from 3 heifers had limited antigenic variation, whereas whole-cell antigens of isolates from 2 cattle (the bull and a heifer) differed serologically from those of the infecting strain. changes were detected specifically in 6 heat-labile antigens. of the 6 ... | 1989 | 2548417 |
| the effect of campylobacter lipopolysaccharide on fetal development in the mouse. | purified lipopolysaccharide (lps) obtained from isolates of campylobacter fetus ss. fetus and campylobacter jejuni impaired fetal development when administered to mice on day 13 of pregnancy. strikingly more fetal resorption was produced by c. jejuni lps than by similar amounts of c. fetus ss. fetus lps. three of the four campylobacter strains examined produced lps that had no effect on maternal health, but lps from one c. jejuni strain killed all of the mice to which it was administered. | 1988 | 2968457 |
| concurrent bovine virus diarrhoea virus and campylobacter fetus infection in an aborted bovine fetus. | 1988 | 3354166 | |
| characterization of freshly isolated campylobacter coli strains and suitability of selective media for their growth. | typical and atypical campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as campylobacter coli by dna homology testing. tetracycline-resistant isolates were obtained from animals with no recorded exposure to antibiotics. a selection of 12 c. coli and 6 c. jejuni strains was used to determine ... | 1988 | 3356790 |
| campylobacter jejuni antibodies in nigerian children. | titers of complement-fixing (cf) antibody to campylobacter jejuni were demonstrated in 87 (36.7%) of 237 infants 6 to 15 months old in jos, nigeria. of the total number of children examined, 81 had acute diarrhea and 27 of them (33.3%) were found to have cf antibodies in their serum. the remaining 156 children were asymptomatic, and 60 (38.4%) of them had cf antibodies. in the diarrheal group, 27 of 75 children 6 to 8 months old were cf antibody positive. there was no significant difference in t ... | 1988 | 3356797 |
| purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus. | a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ... | 1988 | 3360785 |
| improved blood free selective medium for isolating campylobacter jejuni from faecal specimens. | 1988 | 3385002 | |
| structural and biochemical analyses of a surface array protein of campylobacter fetus. | electron microscopic examination of ultrathin sections and freeze-etched and shadow cast preparations of a bovine prepuce isolate of campylobacter fetus vc119 showed an s layer with subunits in an apparent linear arrangement. surface radioiodination, enzyme digestion, low-ph extraction, and western immunoblotting showed that the layer was composed mainly of one protein which is the predominant protein antigen of c. fetus. this protein was purified to homogeneity by gel filtration, ion-exchange c ... | 1988 | 3410826 |
| campylobacters in man and the environment in hull and east yorkshire. | campylobacter organisms isolated from water samples taken weekly from ponds and land-drains in the city of hull were compared with isolates from humans. of 314 campylobacter organisms isolated from patients, 237 (75.5%) of the strains were identified as typical campylobacter jejuni, whilst of 125 identified strains isolated from the water samples, 85 (68%) resembled c. jejuni in most respects but were hippurate hydrolysis negative by the hwang and ederer method. the ponds and land drains in the ... | 1988 | 3181312 |
| role of the 92.5-kilodalton outer membrane protein of campylobacter jejuni in serological reactions. | outer membrane proteins from 12 strains of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with rabbit antisera prepared against formalin-treated cell suspensions of three of the strains. the 92.5-kilodalton protein was confirmed to be a minor outer membrane protein and exhibited serological heterogeneity. serological specificities of the proteins, however, were found not to correlate with serotypic specificities of the strains, ... | 1988 | 3230126 |
| paediatric campylobacter diarrhoea from household exposure to live chickens in lima, peru. | although campylobacter jejuni is a frequent enteropathogen in cases of paediatric diarrhoea in developing countries, its route of transmission is not well understood. an age-matched, case-control study of children with c. jejuni diarrhoea was therefore carried out in lima, peru, from january 1983 to april 1986 to identify the risk factors and vehicles of transmissions. as cases, 104 children less than 3 years of age were selected and compared with controls of the same age with non-gastrointestin ... | 1988 | 3262442 |
| nosocomial meningitis due to campylobacter fetus subsp. fetus in a neonatal intensive care unit. | 1988 | 3197739 | |
| trichomoniasis and campylobacteriosis in bulls in the republic of transkei. | trichomonas foetus was demonstrated in 23/87 (26.4%) and campylobacter fetus in 25/87 (28.7%) of bulls tested in transkei. a total of 16/87 (18.39%) of bulls tested were positive for both trichomonas foetus and campylobacter fetus. bulls from 14 sites in transkei were tested and trichomonas foetus was isolated at 9 of these sites. campylobacter fetus was isolated at 10 of the 14 sites. the results indicate that both trichomonas foetus and campylobacter fetus are widespread throughout the cattle ... | 1988 | 3199409 |
| campylobacter fetus endocarditis on a prosthetic heart valve. | campylobacter fetus infection in man is rare. few cases of campylobacter endocarditis have been described, and none have been associated with a prosthetic heart valve. we have reported a case of campylobacter fetus ssp fetus on a prosthetic mitral valve in a 48-year-old woman who was treated successfully with penicillin and streptomycin (and later with gentamicin). replacement of the prosthetic valve was done because of stenosis and vegetations. pathologic examination showed vegetations on a cal ... | 1988 | 3287646 |
| campylobacters and impaired fetal development in mice. | intravenous injection of eight human strains of campylobacter fetus ss fetus and campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. implantation was significantly impaired when c. fetus ss fetus was injected intravenously on day 2 of pregnancy, but no effect was observed in mice receiving c. jejuni. on day 6 of pregnancy, before the developme ... | 1988 | 2961890 |
| experimental colonization of broiler chicks with campylobacter jejuni. | minimal colonization inocula for two broiler strains of campylobacter jejuni were determined in broiler chicks aged 2-3 days and 2 weeks. individually housed chicks were exposed to a single oral or cloacal challenge. diarrhoeal symptoms were absent in all 380 chicks included in the study. chick susceptibility to the two c. jejuni strains varied. colonization was effected by less than 10(2)-10(4) colony forming units (c.f.u.) via cloacal challenge and 10(4)-10(6) c.f.u. via the oral route. coloni ... | 1988 | 3338504 |
| evaluation of the campyslide agglutination test for confirmatory identification of selected campylobacter species. | the utility of a rapid latex slide agglutination test (campyslide; bbl microbiology systems, cockeysville, md.) in detecting selected campylobacter spp. was evaluated and compared with that of conventional identification methods. isolated colonies suggestive of campylobacter spp. were tested directly from primary selective media after incubation at 42 degrees c under microaerophilic conditions. stock cultures of campylobacter jejuni (n = 27) and c. coli (n = 3) were correctly confirmed to the ge ... | 1988 | 3343315 |
| infertility in cattle in south-west scotland caused by an 'intermediate' strain of campylobacter fetus subspecies fetus (formerly campylobacter fetus intestinalis). | 1988 | 3345413 | |
| pathogenesis of campylobacter fetus infections. failure of encapsulated campylobacter fetus to bind c3b explains serum and phagocytosis resistance. | campylobacter fetus ssp. fetus strains causing systemic infections in humans are highly resistant to normal and immune serum, which is due to the presence of high molecular weight (100,000, 127,000, or 149,000) surface (s-layer) proteins. using serum-resistant parental strains (82-40 lp and 23d) containing the 100,000-mol wt protein and serum-sensitive mutants (82-40 hp and 23b) differing only in that they lack the 100,000-mol wt protein capsule, we examined complement binding and activation, an ... | 1988 | 3366901 |
| cryopreservation studies of campylobacter. | seven strains of campylobacter fetus ss. fetus, one of campylobacter fetus ss. venerealis, and one of campylobacter jejuni were preserved using a variety of cryopreservation methods. organisms were frozen to -150 degrees c in a liquid nitrogen refrigerator, in the freezer compartment of a refrigerator (-20 degrees c), and in a mechanical freezer (-65 degrees c). in the latter two cases, viabilities of the organisms were compared after being frozen in brucella albimi broth and 10% glycerol. viabi ... | 1988 | 3371059 |
| is enrichment culture necessary for isolating campylobacter jejuni from faeces? | 1988 | 3192739 | |
| chemotactic behavior of campylobacter jejuni. | the chemotactic behavior of campylobacter jejuni was determined in the presence of different amino acids, carbohydrates, organic acids, and preparations and constituents of mucin and bile. l-fucose was the only carbohydrate and l-aspartate, l-cysteine, l-glutamate, and l-serine were the only amino acids producing a chemotactic (positive) response. several salts of organic acids, including pyruvate, succinate, fumarate, citrate, malate, and alpha-ketoglutarate, were also chemoattractants, as were ... | 1988 | 3372020 |
| the extent of surface contamination of retailed chickens with campylobacter jejuni serogroups. | eighty-two chickens purchased at 11 retailers (supplied by 12 wholesalers) in the south of england were cultured for campylobacter jejuni by a method involving total immersion. the organism was isolated from 22 (48%) of 46 fresh birds, 12 of 12 uneviscerated (new york dressed) birds, but only 1 of 24 frozen birds. viable counts of up to 1.5 x 10(6)/chicken were obtained from fresh birds and 2.4 x 10(7)/chicken from uneviscerated birds. surface swabbing of breasts, thighs, wings and vents of fres ... | 1988 | 3338503 |
| the immune system response to campylobacter infection. | campylobacter may be one of the most common causes of bacterial gastroenteritis (ge) in children. it has recently been suggested that it is one of the bacterial pathogens most likely to infect immune-compromised children, and it may facilitate colonization of enteric pathogens. the immune system response was studied in 12 children with campylobacter fetus subspecies jejuni (cbj) infections. serum concentrations of iga, igm, and igg were analyzed using a beckman auto-analyzer. sera specific ab to ... | 1988 | 3374406 |
| small-scale dna preparation for rapid genetic identification of campylobacter species without radioisotope. | a simplified and rapid genetic identification method for campylobacter species without radioisotope was established. three different amounts of dna (200, 50, and 12.5 ng) extracted from each type strain of campylobacter species with standard marmur's procedure were spotted on a nitrocellulose filter. dna obtained from one ml bacterial suspension at a concentration of mcfarland standard turbidity no. 1 of campylobacter fetus, c. jejuni, c. coli, and c. pylori isolates were sufficiently labeled wi ... | 1988 | 3374408 |
| mucosal and systemic immunity to campylobacter jejuni in rabbits after gastric inoculation. | the mucosal and systemic immune responses to campylobacter jejuni were studied in rabbits receiving gastric inoculation with live organisms. a lavage procedure was used to facilitate repeated monitoring of the intestinal immune response to c. jejuni. immunity to c. jejuni was determined by secondary challenge by using the removable intestinal tie adult rabbit diarrhea (ritard) model and monitoring for resistance to colonization and bacteremia. oral-gastric inoculation of normal rabbits produced ... | 1988 | 3335413 |
| vaccination of pregnant guinea pigs with campylobacter fetus: effects of antigen dose, campylobacter strain, and adjuvant type. | for the purpose of developing and improving vaccines to protect cattle against bovine genital campylobacteriosis, the interaction of vaccine dose, strain(s) of campylobacter fetus, and type of adjuvant was studied in the pregnant guinea pig model. five strains of c fetus were used in the preparation of 20 monovalent vaccines containing various doses of bacteria (0.1 mg to 20 mg, dry weight) in freund incomplete adjuvant, freund complete adjuvant, or aluminum hydroxide gel adjuvant. vaccinal prot ... | 1988 | 3377303 |
| gene disruption and replacement as a feasible approach for mutagenesis of campylobacter jejuni. | campylobacter jejuni and campylobacter coli are important causes of human enteric infections. several determinants of pathogenicity have been proposed based on the clinical features of diarrheal disease and on the phenotypic properties of campylobacter strains. to facilitate an understanding of the genetic determinants of campylobacter virulence, we have developed a method for constructing c. jejuni mutants by shuttle mutagenesis. in the example described here, a kanamycin resistance gene was in ... | 1988 | 2832375 |
| enteropathogens associated with acute infantile diarrhoea in cape town. | the results of a year-long controlled study on the causation of acute infectious diarrhoea in infants are reported. campylobacter fetus subspecies jejuni, rotavirus and certain enteropathogenic escherichia coli (epec) types were identified as the most common enteropathogens. shigella, salmonella group b and yersinia enterocolitica were significant but less common pathogens. campylobacter, rotavirus, the significant epec types and shigella infections were more frequent in the warm summer and autu ... | 1988 | 3340928 |
| characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. | four isolates of an unclassified microaerophilic bacterium resembling campylobacter species were characterized by growth requirements, microscopic examination, biochemical characteristics, antimicrobial susceptibility tests, and protein profile analysis. the unclassified isolates were differentiated from campylobacter jejuni, campylobacter coli, campylobacter fetus subsp. fetus, campylobacter laridis, campylobacter pylori, and an ovine isolate. the bacterium was fusiform shaped with a corrugated ... | 1988 | 3343302 |
| high-voltage electroporation of bacteria: genetic transformation of campylobacter jejuni with plasmid dna. | electroporation permits the uptake of dna by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. we investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathogen campylobacter jejuni as a model system. this report demonstrates that the application of high-voltage discharges to bacterial cells permits genetic transformation. our method involves exposure of a ... | 1988 | 3277182 |
| isolation of campylobacter jejuni from the bile of a cholecystic patient. | described is the isolation of campylobacter jejuni from the bile of a cholecystic patient admitted for abdominal pain to an intensive care unit. a cholecystectomy was performed, and the patient responded to erythromycin therapy. details of this uncommon isolation are given. | 1988 | 3183005 |
| infection with campylobacter coli and campylobacter fetus in a patient on continuous ambulatory peritoneal dialysis. | 1988 | 3183412 | |
| detection of two different kanamycin resistance genes in naturally occurring isolates of campylobacter jejuni and campylobacter coli. | a total of 225 isolates of campylobacter jejuni and 54 isolates of campylobacter coli were screened for resistance to kanamycin. among these, five resistant isolates of c. jejuni and six resistant isolates of c. coli, all with different plasmid patterns, were identified. each contained at least one plasmid greater than or equal to 41 kilobases in size. the mic of kanamycin for all 11 strains was determined to be greater than or equal to 256 micrograms/ml by an agar dilution method. in addition, ... | 1988 | 3190204 |
| colonization of gastrointestinal tracts of chicks by campylobacter jejuni. | bacterial enumeration and histologic examination of organs and tissues of 8-day-old chicks 7 days after peroral inoculation with campylobacter jejuni revealed that the organism colonized primarily the lower gastrointestinal tract. the principal sites of localization were the ceca, large intestine, and cloaca, where densely packed cells of c. jejuni were observed in mucus within crypts. examination of c. jejuni-colonized crypts by transmission electron microscopy revealed that the campylobacters ... | 1988 | 3060015 |
| a new antibiotic combination for frozen bovine semen 1. control of mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus. | systematic evaluations of new combinations of antibiotics for the control of bovine mycoplasmas, ureaplasmas, campylobacter fetus subsp. venerealis and haemophilus somnus in a bovine frozen semen process were made. these organisms were standardized to 10(5) to 10(6) colony forming unit (cfu) and inoculated into each ml of raw semen. antibiotics in a final volume of 0.02 ml were added to each ml of the raw semen and were contained at the same concentration in the nonglycerol portion of the extend ... | 1988 | 16726381 |
| an examination of campylobacter fetus subsp. fetus by restriction endonuclease analysis and serology. | restriction endonuclease analysis was used to examine 70 different strains of campylobacter fetus subsp. fetus, which were isolated from aborted sheep foetuses. the strains could be divided into seven types based on the dna fragment patterns obtained by electrophoresis after digestion with the enzyme bsteii. with one exception, digestion with the enzyme xhoi allowed the strains to be grouped identically to that for bsteii. antisera were made against formalized whole cells representative of each ... | 1987 | 2820112 |
| characterization and expression of a cloned tetracycline resistance determinant from campylobacter jejuni plasmid pua466. | a tetracycline resistance (tcr) determinant previously cloned from the campylobacter jejuni plasmid pua466 (d. e. taylor, j. bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase hincii fragments. tcr encoded by the cloned fragment (puoa1) was expressed constitutively in escherichia coli. a protein with an apparent molecular weight of 68,000 encoded by puoa1 was pr ... | 1987 | 3036765 |
| antimicrobial resistance of campylobacter jejuni and campylobacter coli with special reference to plasmid profiles of japanese clinical isolates. | a total of 111 clinical isolates of campylobacter jejuni and 10 clinical isolates of campylobacter coli were characterized by their susceptibility to nine antimicrobial agents and by their plasmid profiles on agarose gel electrophoresis. all of the c. jejuni isolates were susceptible to chloramphenicol, ciprofloxacin, erythromycin, kanamycin, and nalidixic acid, but 55% were tetracycline resistant. in the 10 c. coli isolates, a high prevalence of multiple-antibiotic resistance was noted. plasmid ... | 1987 | 3038005 |
| two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines. | the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ... | 1987 | 3038946 |
| acid and alkaline campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits. | acid and alkaline extracts from several campylobacter strains were used as antigens in enzyme immunoassay (eia). antibodies were detected in rabbits immunized with formalin-treated, boiled, or autoclaved whole bacteria of different campylobacter subspecies. for the preparation of the extracts, three campylobacter fetus subsp. venerealis strains, three campylobacter fetus subsp. fetus strains, four campylobacter coli, and five campylobacter jejuni strains were used. the acid extracts of campyloba ... | 1987 | 3296648 |
| multiple enteric pathogens in a patient with diarrhoea. | a woman who recently arrived in canada from india developed a febrile, diarrhoeal illness. stool culture revealed the presence of salmonella typhi, campylobacter fetus subspecies jejuni and shigella flexneri. concurrent infection with these three pathogens does not appear to have been previously reported. the possibility of multiple pathogens should be considered in travellers who fail to respond to treatment of a single organism. | 1987 | 3299934 |
| expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria. | enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ... | 1987 | 3312015 |
| comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis. | the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ... | 1987 | 2954818 |
| gene transfer from escherichia coli to campylobacter species: development of shuttle vectors for genetic analysis of campylobacter jejuni. | a shuttle cloning vector (pil550) has been constructed which can be mobilized from escherichia coli to campylobacter jejuni, campylobacter coli, and campylobacter fetus by complementation with the transfer functions of an incp plasmid in trans, with a frequency of 10(-4) transconjugants per donor. we also present evidence for a dna modification system in c. jejuni. | 1987 | 2822671 |
| cloning and expression of a tetracycline resistance determinant from campylobacter jejuni in escherichia coli. | the tetracycline resistance gene (tet) from the campylobacter jejuni plasmid pfkt1025 was cloned into both puc18 and pbr322 and was expressed when the chimeric plasmids were introduced into escherichia coli. the location of the tet determinant on the chimeric plasmids was determined by bal 31 deletion mapping within a 2.25-kilobase (kb) rsai-hincii fragment. a protein of approximately 70 kilodaltons was consistently produced by e. coli maxicells harboring the cloned tet determinant. a 500-base-p ... | 1987 | 2823694 |
| prevalence and characterization of hippurate-negative campylobacter jejuni in king county, washington. | a total of 593 strains of thermophilic campylobacter species were isolated either from humans with diarrhea or from poultry in king county, washington. of these strains, 98 (52 hippurate-positive strains and all 46 of the hippurate-negative strains) were selected for further phenotypic characterization and genetic classification. hippurate hydrolysis, the test typically used to differentiate campylobacter jejuni and c. coli, did not always correlate with the genetic classification. all hippurate ... | 1987 | 3654945 |
| cytotoxic and enterotoxic activities of campylobacter jejuni are not specified by tetracycline resistance plasmids pmak175 and pua466. | the 45-kilobase tetracycline resistance plasmids pmak175 and pua466 from campylobacter jejuni were examined using curing and mating experiments. however, these plasmids encoded neither cytotoxin production, as determined in vero cells, nor enterotoxin activity, as determined in chinese hamster ovary cells. | 1987 | 3793867 |
| evaluation of four enrichment media for isolation of campylobacter jejuni. | diarrheal stool specimens were inoculated into the following media: alkaline peptone water (apw), bruce-zochowsky medium (bz), campylobacter enrichment broth (ceb), campy-thio broth (ct), and skirrow blood-agar (sk) plate. all media were incubated at 42 degrees c in microaerophilic conditions for 24 h. afterwards, a new sk plate was inoculated from every liquid medium. campylobacter jejuni was isolated from 43 of the 259 specimens when ct was used, from 45 when apw was used, from 46 when bz was ... | 1987 | 3793873 |
| susceptibility of campylobacter jejuni and yersinia enterocolitica to uv radiation. | two enteric pathogens, campylobacter jejuni and yersinia enterocolitica serogroup o:3, together with escherichia coli, were investigated for susceptibility to uv radiation at 254 nm. the uv dose required for a 3-log reduction (99.9% inactivation) of c. jejuni, y. enterocolitica, and e. coli was 1.8, 2.7, and 5.0 mws/cm2, respectively. using e. coli as the basis for comparison, it appears that c. jejuni and y. enterocolitica serogroup o:3 are more sensitive to uv than many of the pathogens associ ... | 1987 | 3551844 |
| [infections caused by campylobacter fetus]. | 1987 | 3561577 | |
| demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus. | western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ... | 1987 | 3818099 |
| intestinal mucus gel and secretory antibody are barriers to campylobacter jejuni adherence to int 407 cells. | an in vitro mucus assay was developed to study the role of mucus gel and secretory immunoglobulin a (siga) in preventing attachment of campylobacter jejuni to int 407 cells. an overlay of rabbit small intestinal mucus was found to impede the attachment of c. jejuni to a monolayer of int 407 cells. mucus from rabbits previously colonized with c. jejuni was found to completely inhibit bacterial adherence to the underlying cells. anti-campylobacter siga was readily detected in mucus samples from pr ... | 1987 | 3570473 |
| isolation of campylobacter spp. from pigeon feces by a combined enrichment-filtration technique. | a technique combining enrichment in preston enrichment broth and direct filtration onto chocolate agar was used to isolate campylobacter species from pigeon feces. campylobacter jejuni was isolated from 106 of 200 samples tested; 105 strains were isolated by enrichment-filtration, and 84 strains were isolated by direct plating. most of the strains grew after 48 h at 37 degrees c. | 1987 | 3606114 |
| kinetics of anti-campylobacter jejuni monomeric and polymeric immunoglobulin a1 and a2 responses in serum during acute enteritis. | the intensity and kinetics of the serum polymeric and monomeric immunoglobulin a1 (iga1) and iga2 antibody responses to campylobacter jejuni were analyzed. a rapid and marked serum iga antibody response involving both the monomeric and polymeric components of iga was observed after c. jejuni infections. iga antibodies reached a peak of activity in serum during week 2 after the first symptoms of enteritis, about 10 days before the peak of igg activity. polymeric iga accounted for most of the anti ... | 1987 | 3611318 |
| bovine abortion due to campylobacter fetus. | 1987 | 3439950 | |
| structural and antigenic properties of lipopolysaccharides from serotype reference strains of campylobacter jejuni. | to investigate the molecular basis for heat-stable antigenic diversity in campylobacter jejuni, lipopolysaccharides (lpss) from serotype reference strains and serotyped isolates were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis coupled with silver staining and immunoblotting. by silver staining, only low-mr components, consisting of one major band and as many as three minor bands ranging in mr from 4,500 to 5,000, were detected. however, by immunoblotting with homologous ... | 1987 | 3301678 |
| meningitis due to campylobacter fetus intestinalis in a kidney transplant recipient. a case report. | a 47-year-old man developed campylobacter fetus intestinalis meningitis 3 years after cadaver renal transplantation. the infection was successfully controlled with chloramphenicol followed by erythromycin. there were no relapses during the past 5 years and the allograft function remains normal. the available evidence suggests that the campylobacter sepsis was associated with nutritional therapy, the patient received in a mexican clinic. the present case is reported because of its clinical import ... | 1987 | 3324765 |
| a comparative study of the growth of campylobacter fetus strains in liquid media. | the growth of c. fetus fetus, c. fetus venerealis and c. fetus venerealis biotype intermedius was examined in 10 liquid media. from the data obtained, a 10% inoculum size and an oxygen level of 6% seemed imperative for consistent growth, especially for the c. fetus venerealis strain. a lowered redox potential obtained by the addition of 0, 1% cysteine-hc1 to the media was stimulatory. the medium which yielded the best growth was the one described by dennis & jones (1959). the fastidious c. fetus ... | 1987 | 3328136 |
| rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by campylobacter jejuni and campylobacter coli. | a gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by campylobacter spp. this technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chromatographic method for identification of campylobacter isolates. | 1987 | 3654950 |
| utilization of monoclonal antibodies to evaluate the involvement of campylobacter jejuni in proliferative ileitis in syrian hamsters (mesocricetis auratus). | the role of campylobacter jejuni in the pathogenesis of proliferative ileitis in syrian hamsters was evaluated with monoclonal antibodies of different specificities. monoclonal antibodies were produced with two different specificities: one for all members of the genus campylobacter tested (antibody 8322-2e6) and one for c. jejuni and campylobacter coli (antibodies 841-2a11, 841-4c6, and 841-5b1). heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, a ... | 1987 | 3623701 |
| evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents. | sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ... | 1987 | 3651881 |
| production of a unique cytotoxin by campylobacter jejuni. | campylobacter jejuni is an important diarrheal pathogen worldwide; the mechanisms by which it causes disease remain unclear. because of its association with inflammatory diarrhea, we postulated that c. jejuni might produce a cytotoxin similar to that produced by shigella sp., enterohemorrhagic escherichia coli o157, or clostridium difficile. filtrates of 12 polymyxin-treated isolates of c. jejuni were placed on hela cells (sensitive to shiga toxin cytotoxicity) and chinese hamster ovary (cho) ce ... | 1987 | 3653987 |
| naturally occurring auxotrophs of campylobacter jejuni and campylobacter coli. | the nutritional requirements for 439 campylobacter jejuni isolates and 46 campylobacter coli isolates were determined by using a previously described chemically defined medium, campylobacter defined medium. with this medium, 45% of both human and nonhuman c. jejuni isolates demonstrated auxotrophic requirements. none of the 46 c. coli isolates studied demonstrated requirements for amino acids on campylobacter defined medium. the most common auxotrophic requirement among c. jejuni isolates was fo ... | 1987 | 3654939 |
| atypical campylobacters associated with gastroenteritis. | nine strains of campylobacter species other than campylobacter jejuni, campylobacter coli, and campylobacter laridis were isolated from patients with acute diarrhea. all nine strains showed preferred growth at 37 degrees c under microaerophilic conditions. conventional microbiological tests and dna-dna dot blotting were used to identify these strains. three of the nine campylobacter strains hydrolyzed hippurate, reduced nitrate, produced catalase, were resistant to cephalothin, and were shown to ... | 1987 | 3611317 |
| variations in the virulence, for pregnant guinea pigs, of campylobacters isolated from man. | pregnant guinea pigs were used to compare the virulence of four human isolates of campylobacter fetus ss. fetus and four of c. jejuni on the basis of their ability to cause abortion and bacteraemia. of the four strains of c. fetus ss. fetus two produced abortion readily after intramuscular injection. the four c. jejuni isolates were, however, of comparatively low virulence and no differences between them were demonstrated. some of the isolates differed in their ability to survive in vitro in hum ... | 1987 | 3560189 |
| campylobacter-associated appendicitis: prevalence and clinicopathologic features. | to determine the prevalence of campylobacter fetus subspecies jejuni-associated appendicitis, we studied, retrospectively, by means of immunohistochemistry, the appendectomy specimens of 116 consecutive patients, operated upon because of suspected acute appendicitis. we found immunohistochemical evidence of campylobacter fetus subspecies jejuni infection in three patients. these findings were confirmed by electron microscopy. based upon these three cases and five additional appendectomy specimen ... | 1987 | 3574983 |
| seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria. | campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ... | 1987 | 3579268 |
| campylobacter jejuni in dairy cows and raw milk. | twelve herds of dairy cows were examined by rectal swabbing for the presence of campylobacter jejuni. ten herds were positive with the incidence of colonized animals ranging from 10 to 72% of those tested. with the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. there was no relationship between total and coliform counts and the presence of c. jejuni in raw milk. however, milk from one farm that consistently gave pos ... | 1987 | 3595744 |
| the demonstration of the k-antigen of campylobacter fetus venerealis using a microtitre agglutination test. | using specific antisera prepared against heat-treated and untreated cells in a microtitre system, 7 strains of campylobacter fetus venerealis were examined for the presence of k-antigen. the technique was effective for distinguishing between strains and it was found that 4 out of 7 isolates contained microcapsules, therefore being potentially suitable for vaccine production. | 1987 | 3444618 |
| in vitro activity of a-56268 (te-031), a new macrolide, compared with that of erythromycin and clindamycin against selected gram-positive and gram-negative organisms. | the in vitro activity of a-56268 was determined and compared with that of erythromycin and clindamycin against a limited spectrum of 401 gram-positive and gram-negative organisms. a-56268 was quite active against erythromycin-susceptible staphylococcus aureus, neisseria gonorrhoeae, listeria monocytogenes, streptococcus pneumoniae, streptococcus pyogenes, and group b streptococci and was moderately active against campylobacter fetus subsp. fetus. a-56268 was consistently bactericidal only for s. ... | 1987 | 2952063 |
| [campylobacter fetus bursitis of the elbow]. | 1987 | 3672019 | |
| survival of thermophilic campylobacters on fingertips and their elimination by washing and disinfection. | a simple impression-plate technique has been used to investigate the survival of four thermophilic campylobacter strains applied to fingertips. campylobacters suspended in 0.1% peptone water and dried on the fingertips survived for different periods of time ranging from less than 1 to greater than or equal to 4 min. however, campylobacters suspended in chicken liquor or blood survived for much longer periods. the most resilient organism was campylobacter jejuni nctc 11392 which, when suspended i ... | 1987 | 3678391 |
| problems in identification of campylobacter jejuni associated with acquisition of resistance to nalidixic acid. | in a patient with campylobacteriosis, we observed development of resistance to nalidixic acid and norfloxacin under adequate treatment with the latter antibiotic. isolates before and after treatment differed in their total protein profile but were otherwise identical with respect to enzymatic activity, serotype, crude membrane protein profiles, and other phenotypic characteristics. | 1987 | 3654954 |
| treatment of campylobacter gastroenteritis. | twin boys suffered from recurrent diarrhoea due to campylobacter jejuni after entering a day nursery. stool sampling of all 17 children at the nursery revealed c. jejuni in 12. simultaneous treatment with antibiotics of all children with positive cultures successfully eradicated the infection. | 1987 | 3813645 |
| pathogenesis of campylobacter fetus infections: serum resistance associated with high-molecular-weight surface proteins. | campylobacter fetus subspecies fetus causes both systemic and diarrheal illnesses. we studied 38 strains of c. fetus isolated from 34 patients; underlying illness was present in eight (89%) of nine patients with only systemic isolates compared with three (20%) of 15 patients with only fecal isolates (p = .002). in a standardized assay of susceptibility to normal human serum, 27 (71%) strains were resistant, six (16%) had intermediate susceptibility, and five (13%) were serum sensitive. major pro ... | 1987 | 3819475 |
| [septicemia caused by enteritis due to campylobacter fetus subspecies fetus]. | in a 55 year old man with renal insufficiency requiring hemodialysis after bilateral nephrectomy who had been hospitalized with febrile illness and enteritis, campylobacter fetus subspecies fetus could be isolated from the blood and feces. antibodies against the homologous strain (isolated from the blood) could be detected in the serum by means of the widal test and the indirect immunofluorescence test. culturing of campylobacter fetus subspecies fetus (especially from blood cultures) may be dif ... | 1987 | 3829936 |