Publications

expression profile of the copper homeostasis gene, ratox1, in the rat brain.	in humans the regulation of cellular copper homeostasis is essential for proper organ development and function. a novel cytosolic protein, named atox 1, was recently identified in yeast that functions in shuttling intracellular mononuclear copper [cu(i)] to copper-requiring proteins. atox 1 and its human homolog, hatox1, are members of an emerging family of proteins termed copper chaperones that are involved in the maintenance of copper homeostasis. northern blot analysis demonstrates that atox ...	1999	10473283
identification of an nadh-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, mortierella alpina 1s-4, by sequencing of the encoding cdna and heterologous expression in a fungus, aspergillus oryzae.	based on the sequence information for bovine and yeast nadh-cytochrome b(5) reductases (cbrs), a dna fragment was cloned from mortierella alpina 1s-4 after pcr amplification. this fragment was used as a probe to isolate a cdna clone with an open reading frame encoding 298 amino acid residues which show marked sequence similarity to cbrs from other sources, such as yeast (saccharomyces cerevisiae), bovine, human, and rat cbrs. these results suggested that this cdna is a cbr gene. the results of a ...	1999	10473389
rat tumour necrosis factor-alpha: expression in recombinant pichia pastoris, purification, characterization and development of a novel elisa.	tumour necrosis factor-alpha is a pro-inflammatory cytokine involved in many aspects of acute phase and immune responses. species specificity in the biological action and receptor binding of tnf-alpha make it desirable to use homologous reagents in experimental models, both in vivo and in vitro. as the rat is the model of choice in many investigations on fever, trauma and pathology, there is a need for specific rat reagents. in this paper, we describe the production of recombinant rat tnf-alpha ...	1999	10477395
human liver-specific very-long-chain acyl-coenzyme a synthetase: cdna cloning and characterization of a second enzymatically active protein.	activation of fatty acids, catalyzed by acyl-coenzyme a (acyl-coa) synthetases, is required for their subsequent metabolism. peroxisomes and microsomes contain very-long-chain acyl-coa synthetases (vlcss) capable of activating fatty acids with a chain length of 22 or more carbons. decreased peroxisomal vlcs activity is, in part, responsible for the biochemical pathology in x-linked adrenoleukodystrophy (x-ald), illustrating the importance of vlcss in cellular fatty acid homeostasis. we previousl ...	1999	10479480
sertolin is a novel gene marker of cell-cell interactions in the rat testis.	a novel testicular protein designated sertolin was cloned. the full-length sertolin cdna consists of 853 base pairs with an open reading frame of 381 base pairs coding for a 127-amino acid polypeptide that shares limited identities with antaxin/josephin and thrombospondin proteins. sertolin (calculated molecular mass, 13,759 daltons) has two mrna transcripts of 2.3 and 1 kilobase. a 22-amino acid peptide based on the deduced amino acid sequence of sertolin (nh(2)-kkehfnlfkaasvshlvqvvpq) was synt ...	1999	10480919
molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids.	to study the putative role of human carnitine octanoyltransferase (cot) in the beta-oxidation of branched-chain fatty acids, we identified and cloned the cdna encoding human cot and expressed it in the yeast saccharomyces cerevisiae. enzyme activity measurements showed that cot efficiently converts one of the end products of the peroxisomal beta-oxidation of pristanic acid, 4, 8-dimethylnonanoyl-coa, to its corresponding carnitine ester. production of the carnitine ester of this branched/medium- ...	1999	10486279
cooperation of six and eya in activation of their target genes through nuclear translocation of eya.	drosophila sine oculis and eyes absent genes synergize in compound-eye formation. the murine homologues of these genes, six and eya, respectively, show overlapping expression patterns during development. we hypothesized that six and eya proteins cooperate to regulate their target genes. cotransfection assays were performed with various combinations of six and eya to assess their effects on a potential natural target, myogenin promoter, and on a synthetic promoter, the thymidine kinase gene promo ...	1999	10490620
emk protein kinase-null mice: dwarfism and hypofertility associated with alterations in the somatotrope and prolactin pathways.	gene trapping was used in embryonic stem (es) cells in an attempt to inactivate genes involved in development. the emk (elkl motif kinase) gene has been disrupted and a mutant mouse line derived. previous work had shown that emk kinases, called mark in the rat, exert a major control on microtubule stability by phosphorylating microtubule-associated proteins and that genes homologous to emk in yeast or caenorhabditis elegans are essential for cell and embryonic polarity. although we found the emk ...	1999	10491259
cloning and expression of ctp:phosphoethanolamine cytidylyltransferase cdna from rat liver.	ctp:phosphoethanolamine cytidylyltransferase (et) is a key regulatory enzyme in the cdp-ethanolamine pathway for phosphatidylethanolamine synthesis. as a first step in the elucidation of the structure-function relationship and the regulation of et, an et cdna was cloned from rat liver. the cloned cdna encodes a protein of 404 amino acid residues with a calculated molecular mass of 45.2 kda. the deduced amino acid sequence is very similar to that of human et (89% identity). furthermore, it shows ...	1999	10493918
chicken ovalbumin upstream promoter-transcription factor ii, a new partner of the glucose response element of the l-type pyruvate kinase gene, acts as an inhibitor of the glucose response.	transcription of the l-type pyruvate kinase (l-pk) gene is induced by glucose in the presence of insulin and repressed by glucagon via cyclic amp. the dna regulatory sequence responsible for mediating glucose and cyclic amp responses, called glucose response element (glre), consists of two degenerated e boxes spaced by 5 base pairs and is able to bind basic helix-loop-helix/leucine zipper proteins, in particular the upstream stimulatory factors (usfs). from ex vivo and in vivo experiments, it ap ...	1999	10497199
reconstitution of fibroblast growth factor receptor interactions in the yeast two hybrid system.	fibroblast growth factors (fgf) activate their receptors through the formation of trimolecular complexes, composed of a ligand, a receptor, and a heparan sulfate oligosaccharide, all of which are members of particularly large families capable of multiple interactions in a combinatorial fashion. understanding this large network of interactions not only presents a great challenge, but is practically beyond the capacity of most classical techniques routinely used to study ligand receptor interactio ...	1999	10503237
disruption of six orfs on saccharomyces cerevisiae chromosome x: the yjl069c gene of unknown function is essential to cell viability.	the short flanking homology pcr strategy (wach et al., 1994) was used to disrupt six open reading frames (orfs) on chromosome x of diploid strains (fy1679 and w303) of the yeast saccharomyces cerevisiae. two of the six orfs analysed (yjl069c and yjl066c) display no similarity to known sequences. three others (yjl065c, yjl068c, and yjl070c) are similar to those respectively encoding the dna polymerase epsilon subunit c, human esterase d and rat amp deaminase 1. yjl071w has recently been identifie ...	1999	10509023
a functional and quantitative mutational analysis of p53 mutations in yeast indicates strand biases and different roles of mutations in dmba- and bbn-induced tumors in rats.	in order to analyze the mutational events and to understand the biological significance of the p53 gene in chemical carcinogenesis, we applied a new yeast-based p53 functional assay to ovarian tumors induced by 7, 12-dimethylbenz[a]anthracene (dmba), as well as to transitional cell carcinomas of the urinary bladder induced by n-butyl-n-(4-hydroxybutyl) nitrosamine (bbn) in rats. the assay demonstrated that 15 of 19 dmba induced tumors harbored clonal p53 mutations, which is consistent with the e ...	1999	10521810
vap-33 localizes to both an intracellular vesicle population and with occludin at the tight junction.	tight junctions create a regulated intercellular seal between epithelial and endothelial cells and also establish polarity between plasma membrane domains within the cell. tight junctions have also been implicated in many other cellular functions, including cell signaling and growth regulation, but they have yet to be directly implicated in vesicle movement. occludin is a transmembrane protein located at tight junctions and is known to interact with other tight junction proteins, including zo-1. ...	1999	10523508
interaction of the metalloprotease disintegrins mdc9 and mdc15 with two sh3 domain-containing proteins, endophilin i and sh3px1.	metalloprotease disintegrins (a disintegrin and metalloprotease (adam) and metalloprotease, disintegrin, cysteine-rich proteins (mdc)) are a family of membrane-anchored glycoproteins that function in diverse biological processes, including fertilization, neurogenesis, myogenesis, and ectodomain processing of cytokines and other proteins. the cytoplasmic domains of adams often include putative signaling motifs, such as proline-rich sh3 ligand domains, suggesting that interactions with cytoplasmic ...	1999	10531379
heterologous gene expression in a membrane-protein-specific system.	we have constructed an expression system for heterologous proteins which uses the molecular machinery responsible for the high level production of bacteriorhodopsin in halobacterium salinarum. cloning vectors were assembled that fused sequences of the bacterio-opsin gene (bop) to coding sequences of heterologous genes and generated dna fragments with cloning sites that permitted transfer of fused genes into h. salinarum expression vectors. gene fusions include: (i) carboxyl-terminal-tagged bacte ...	1999	10545281
biochemical characterization and expression analysis of neural thrombospondin-1-like proteins nell1 and nell2.	two closely related genes coding for nell proteins (nell1 and nell2) have been cloned by the yeast two-hybrid screening of a rat brain cdna library with the regulatory domain of protein kinase c betai (pkcbetai) as bait. the rat nell proteins show about 55% identity with each other and contain several protein motifs assigned to a secretion signal peptide, an nh(2)-terminal thrombospondin-1 (tsp-1)-like module, five von willebrand factor c domains, and six epidermal growth factor-like domains; th ...	1999	10548494
identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity.	p21-activated kinases (pak)/ste20 kinases are regulated in vitro and in vivo by the small gtp-binding proteins rac and cdc42 and lipids, such as sphingosine, which stimulate autophosphorylation and phosphorylation of exogenous substrates. the mechanism of pak activation by these agents remains unclear. we investigated pak kinase activation in more detail to gain insight into the interplay between the gtpase/sphingosine binding, an intramolecular inhibitory interaction, and autophosphorylation. w ...	1999	10551809
molecular cloning of rat atx1 homologue protein.	an atx1 homologue of 503 bp length was cloned from a rat cdna library, and the deduced protein from the cdna was found to contain 68 amino acids with a predicted molecular mass of 7.2 kda. the rat atx1 homologue protein (rah1p), which shows 35%, 38%, and 89% identities with atx1p, cuc-1, and hah1, respectively, conserves both the mtcxxc copper-binding site in the n terminus and the ktgk lysine-rich region in the c terminus. in northern blot analysis, rah1 mrna was found to be expressed at high l ...	1999	10558899
receptor-independent activators of heterotrimeric g-protein signaling pathways.	heterotrimeric g-protein signaling systems are activated via cell surface receptors possessing the seven-membrane span motif. several observations suggest the existence of other modes of stimulus input to heterotrimeric g-proteins. as part of an overall effort to identify such proteins we developed a functional screen based upon the pheromone response pathway in saccharomyces cerevisiae. we identified two mammalian proteins, ags2 and ags3 (activators of g-protein signaling), that activated the p ...	1999	10559191
functional analysis of aquaporin-2 mutants associated with nephrogenic diabetes insipidus by yeast expression.	mutations of aquaporin-2 (aqp2) vasopressin water channel cause nephrogenic diabetes insipidus (ndi). it has been suggested that impaired routing of aqp2 mutants to the plasma membrane causes the disease; however, no determinations have been made of mutation-induced alterations of aqp2 channel water permeability. to address this issue, a series of aqp2 mutants were expressed in yeast, and the osmotic water permeability (p(f)) of the isolated vesicles was measured. wild-type and mutant aqp2 were ...	1999	10564236
suppression of microphthalmia transcriptional activity by its association with protein kinase c-interacting protein 1 in mast cells.	microphthalmia (mi) is a transcription factor that plays a major role in the regulation of growth and function in mast cells and melanocytes. association of mi with other proteins is a critical step in the regulation of mi-mediated transcriptional activation. we found protein kinase c-interacting protein 1 (pkci) specifically associated with mi in yeast two-hybrid screening. immunoprecipitation of mi from quiescent rat basophilic leukemic cells or mouse melanocytes resulted in the specific co-im ...	1999	10567402
specific interaction of the 70-kda heat shock cognate protein with the tetratricopeptide repeats.	using a yeast two-hybrid system with the 70-kda heat shock cognate protein (hsc70) or its c-terminal 30-kda domain as baits, we isolated several proteins interacting with hsc70, including hip/p48 and p60/hop. both are known to interact with hsc70. except for hip/p48, all of the proteins that we isolated interact with the 30-kda domain. moreover, the eevd motif at the c terminus of the 30-kda domain appears essential for this interaction. sequence analysis of these hsc70-interacting proteins reve ...	1999	10567422
neural restrictive silencer factor recruits msin3 and histone deacetylase complex to repress neuron-specific target genes.	accumulative evidence suggests that more than 20 neuron-specific genes are regulated by a transcriptional cis-regulatory element known as the neural restrictive silencer (nrs). a trans-acting repressor that binds the nrs, nrsf [also designated re1-silencing transcription factor (rest)] has been cloned, but the mechanism by which it represses transcription is unknown. here we show evidence that nrsf represses transcription of its target genes by recruiting msin3 and histone deacetylase. transfect ...	1999	10570134
the novel kinase peptidylglycine alpha-amidating monooxygenase cytosolic interactor protein 2 interacts with the cytosolic routing determinants of the peptide processing enzyme peptidylglycine alpha-amidating monooxygenase.	the cytosolic domain of the peptide-processing integral membrane protein peptidylglycine alpha-amidating monooxygenase (pam; ec 1.14. 17.3) contains multiple signals determining its subcellular localization. three pam cytosolic interactor proteins (p-cips) were identified using the yeast two hybrid system (alam, m. r., caldwel, b. d., johnson, r. c., darlington, d. n., mains, r. e., and eipper, b. a. (1996) j. biol. chem. 271, 28636-28640); the partial amino acid sequence of p-cip2 suggested tha ...	1999	10574929
yeast smf1 mediates h(+)-coupled iron uptake with concomitant uncoupled cation currents.	yeast membrane proteins smf1, smf2, and smf3 are homologues of the dct1 metal ion transporter family. their functional characteristics and the implications of these characteristics in vivo have not yet been reported. here we show that smf1 expressed in xenopus oocytes mediates h(+)-dependent fe(2+) transport and uncoupled na(+) flux. smf1-mediated fe(2+) transport exhibited saturation kinetics (k(m) = 2.2 microm), whereas the na(+) flux did not, although both processes were electrogenic. smf1 is ...	1999	10574989
from yeast to man--from mitochondria to liver regeneration: a new essential gene family.	the purpose of this review is to bring to the attention of the reader the latest developments in research on an important new emerging gene family. the respective genes are found in eukaryotes from yeast to man and even on the genome of some doubled-stranded dna viruses. they have essential functions in the biogenesis of mitochondria, the cell division cycle and, in higher eukaryotes, in the development of organs like liver and testis. the most important medical implication is their probable rol ...	1999	10575569
the profile of fr140423, a novel anti-inflammatory compound, in yeast-induced rat hyperalgesia.	the mechanism of action of fr140423 (3-(difluoromethyl)-1-(4-methoxyphenyl)-5-[4-(methylsulfinyl)phenyl]pyra zole), a novel anti-inflammatory compound, in a rat yeast-induced hyperalgesic model was investigated and compared with those of indomethacin and morphine. we tested the inhibitory effects of fr140423 on the formation of arachidonic acid metabolites, prostaglandin (pg) e2, thromboxane (tx) b2 and leukotriene (lt) b4, in yeast-injected inflamed paws and the effect of the opioid receptor an ...	1999	10580376
the dynamin-like protein dlp1 is essential for normal distribution and morphology of the endoplasmic reticulum and mitochondria in mammalian cells.	the dynamin family of large gtpases has been implicated in vesicle formation from both the plasma membrane and various intracellular membrane compartments. the dynamin-like protein dlp1, recently identified in mammalian tissues, has been shown to be more closely related to the yeast dynamin proteins vps1p and dnm1p (42%) than to the mammalian dynamins (37%). furthermore, dlp1 has been shown to associate with punctate vesicles that are in intimate contact with microtubules and the endoplasmic ret ...	1999	10588666
mammalian mitochondrial ribosomal proteins (2). amino acid sequencing, characterization, and identification of corresponding gene sequences.	four different classes of mammalian mitochondrial ribosomal proteins were identified and characterized. mature proteins were purified from bovine liver and subjected to n-terminal or matrix-assisted laser-desorption mass spectroscopic amino acid sequencing after tryptic in-gel digestion and high pressure liquid chromatography separation of the resulting peptides. peptide sequences obtained were used to virtually screen expressed sequence tag data bases from human, mouse, and rat. consensus cdnas ...	1999	10593885
identification and characterization of cvhsp. a novel human small stress protein selectively expressed in cardiovascular and insulin-sensitive tissues.	starting with computational tools that search for tissue-selective expression of assembled expressed sequenced tags, we have identified by focusing on heart libraries a novel small stress protein of 170 amino acids that we named cvhsp. cvhsp was found as being computationally selectively and highly (0.3% of the total rna) expressed in human heart. the cvhsp gene mapped to 1p36.23-p34.3 between markers d1s434 and d1s507. the expression of cvhsp was analyzed with rna dot, northern blots, or revers ...	1999	10593960
interaction partners of dlk/zip kinase: co-expression of dlk/zip kinase and par-4 results in cytoplasmic retention and apoptosis.	dlk/zip kinase is a newly discovered serine/threonine kinase which, due to its homology to dap kinase, was named dap like kinase, dlk. this kinase is tightly associated with nuclear structures, it undergoes extensive autophosphorylation and phosphorylates myosin light chain and core histones h3, h2a and h4 in vitro. moreover, it possesses a leucine zipper which mediates interaction with transcription factor atf-4, therefore it was called zip kinase. we employed the yeast two-hybrid system to ide ...	1999	10602480
comparison in metabolic activity of cytochrome p450 1a1 on heterocyclic amines between human and rat.	in mutagenicity and antimutagenicity tests, the toxicants have been activated to the ultimate mutagenic forms usually with rat cytochrome p450 (cyp) enzymes. an understanding is important of whether these data can be available for human. in this paper are compared the activating abilities of cyp1a1 between human and rat using recombinant yeast cells that express respective cyp1a1 and yeast nadph-cyp-oxidoreductase simultaneously. three different types of dietary procarcinogens, heterocyclic amin ...	1999	10606558
[selenoproteins in rats with chronic selenium intoxication].	weaning male wistar rats were fed with a torula-yeast based semisynthetic diet supplemented with na2seo3 to provide selenium (se) 0.2 or 0.5 mg/kg (adequate se or high sediet) respectively for 20 weeks. by the end of experiment, rats were sacrificed and various tissue of rats were collected to determine the activities of se-containing enzymes and the mrnas level of selenoprotein p and selenoprotein w and se concentration. livers were examined for pathological changes. it was found that the gain ...	1999	12712720
adenovirus type 5 e1a immortalizes primary rat cells expressing wild-type p53.	adenovirus (ad) e1a induces apoptosis in cells expressing wild-type p53, and stable transformation by ad e1a requires the co-introduction of an anti-apoptotic gene such as ad e1b 19k. thus, cells immortalized by ad e1a alone might have lost functional p53. in order to analyze the p53 in rat cells expressing ad e1a, we established rat cell lines by transfecting primary rat embryo fibroblast (ref) and baby rat kidney (brk) cells with cloned ad5 e1a. by using a yeast functional assay, we analyzed p ...	1999	10609613
intermolecular and interdomain interactions of a dynamin-related gtp-binding protein, dnm1p/vps1p-like protein.	dnm1p/vps1p-like protein (dvlp) is a mammalian member of the dynamin gtpase family, which is classified into subfamilies on the basis of the structural similarity. mammalian dynamins constitute the dynamin subfamily. dvlp belongs to the vps1 subfamily, which also includes yeast vps1p and dnm1p. typical structural features that discriminate between members of the vps1 and dynamin subfamilies are that the former lacks the pleckstrin homology and pro-rich domains. dynamin exists as tetramers under ...	1999	9915810
cloning and characterization of prax-1. a new protein that specifically interacts with the peripheral benzodiazepine receptor.	using a cytoplasmic domain of the peripheral benzodiazepine receptor (pbr) as a bait in the yeast two-hybrid system, we have isolated a cdna encoding a new protein that specifically interacts with pbr. we named it prax-1, for peripheral benzodiazepine receptor-associated protein 1. prax-1 is a 1857-amino acid protein, the sequence of which was structurally unrelated to any known proteins. the gene encoding prax-1 is located in the q22-q23 region of the long arm of the human chromosome 17. the pr ...	1999	9915832
atp inhibition of katp channels: control of nucleotide sensitivity by the n-terminal domain of the kir6.2 subunit.	1. to gain insight into the role of the cytoplasmic regions of the kir6.2 subunit in regulating channel activity, we have expressed the sulphonylurea receptor sur1 with kir6.2 subunits containing systematic truncations of the n- and c-termini. up to 30 amino acids could be truncated from the n-terminus, and up to 36 amino acids from the c-terminus without loss of functional channels in co-expression with sur1. furthermore, kir6.2deltac25 and kir6. 2deltac36 subunits expressed functional channels ...	1999	9925874
direct interaction of microtubule- and actin-based transport motors.	the microtubule network is thought to be used for long-range transport of cellular components in animal cells whereas the actin network is proposed to be used for short-range transport, although the mechanism(s) by which this transport is coordinated is poorly understood. for example, in sea urchins long-range ca2+-regulated transport of exocytotic vesicles requires a microtubule-based motor, whereas an actin-based motor is used for short-range transport. in neurons, microtubule-based kinesin mo ...	1999	9930703
a transforming mutation enhances the activity of the c-kit soluble tyrosine kinase domain.	an activating mutation (dy814) located in the catalytic domain of the c-kit receptor has been found in mastocytomas from human, mouse and rat. we evaluated the enzymic properties of purified wild-type (wt) and dy814 tyrosine kinase domains expressed in pichia pastoris. a linker encoding the flag epitope was fused to c-kit cdna species, enabling affinity purification of the proteins with anti-flag antibodies. yeast lysates expressing dy814 contained multiple tyrosine-phosphorylated proteins, wher ...	1999	9931308
chromosomal localization of phospholipase a2 activating protein, an ets2 target gene, to 9p21.	a murine ets2 target gene isolated by differential display cloning was identified as the phospholipase a2 activating protein (plaa) gene. a 2.7-kb human cdna demonstrating high homology to mouse and rat plaa genes was then isolated and characterized. human plaa contains six wd-40 repeat motifs and three different protein kinase consensus domains. fluorescence in situ hybridization (fish) mapping placed plaa on chromosome 9p21, a region frequently deleted in various cancers. a comprehensive mappi ...	1999	10644453
pap i interacts with itself, pap ii, pap iii, and lithostathine/regialpha.	pap i, pap ii, pap iii, and lithostathine/regialpha are members of a multigenic family of proteins expressed in several tissues. pap i was shown to be antiapoptotic, mitogenic, and anti-inflammatory and can promote cell adhesion to the extracellular matrix. lithostathine/regialpha can be mitogenic. because polymerization might regulate activity, we examined the ability of rat pap i to interact with itself (homodimerization), pap ii, pap iii, and lithostathine/regialpha (heterodimerization) by th ...	1999	10662590
yotiao protein, a ligand for the nmda receptor, binds and targets camp-dependent protein kinase ii(1).	a yeast two-hybrid screen revealed that regulatory subunits (rii) of pkaii bind the yotiao protein. yotiao interacts with the nr1 subunit of the nmda receptor. a purified c-terminal fragment of yotiao binds pkaii, via an rii binding site constituted by amino acid residues 1452-1469, with a dissociation constant (k(d)) between 50 and 90 nm in vitro. a stable complex composed of yotiao, rii and nr1 was immunoprecipitated from whole rat brain extracts. immunostaining analysis disclosed that yotiao, ...	1999	10618500
the nuclear cap-binding complex is a novel target of growth factor receptor-coupled signal transduction.	in an attempt to further understand how nuclear events (such as gene expression, nuclear import/export, and cell cycle checkpoint control) might be subject to regulation by extracellular stimuli, we sought to identify nuclear activities under growth factor control. using a sensitive photoaffinity labeling assay that measured [alpha-32p]gtp incorporation into nuclear proteins, we identified the 20-kda subunit of the nuclear cap-binding complex (cbc) as a protein whose binding activity is greatly ...	1999	9933612
mammalian homologue of the caenorhabditis elegans unc-76 protein involved in axonal outgrowth is a protein kinase c zeta-interacting protein.	by the yeast two-hybrid screening of a rat brain cdna library with the regulatory domain of protein kinase c zeta (pkczeta) as a bait, we have cloned a gene coding for a novel pkczeta-interacting protein homologous to the caenorhabditis elegans unc-76 protein involved in axonal outgrowth and fasciculation. the protein designated fez1 (fasciculation and elongation protein zeta-1) consisting of 393 amino acid residues shows a high asp/glu content and contains several regions predicted to form amph ...	1999	9971736
phosphatidylinositol synthesis in mycobacteria.	the metabolism and synthesis of an important mycobacterial lipid component, phosphatidylinositol (pi), and its metabolites, was studied in mycobacterium smegmatis and m. smegmatis subcellular fractions. little is known about the synthesis of pi in prokaryotic cells. only a cell wall fraction (p60) in m. smegmatis was shown to possess pi synthase activity. product was identified as pi by migration on tlc, treatment with phospholipase c and ion exchange chromatography. pi was the only major produc ...	1999	9989274
peripheral blood neutrophils of germ-free rats modified by in vivo granulocyte-colony-stimulating factor and exposure to natural environment.	peripheral blood neutrophils from germ-free (gf), specific-pathogen-free (spf) and conventional (cv) rats were compared. besides neutropenia and impaired superoxide anion generation as previously reported, it was found that gf rats had lower phagocytic function (70%) and generated less nitric oxide than the other rats. gf and spf rats were injected with recombinant human granulocyte-colony-stimulating factor (rhg-csf), or were transferred to natural environment. it was found that total number, p ...	1999	10023860
synergistic activation of yeast-expressed rat androgen receptor by modulators of protein kinase-a.	we have employed a yeast (saccharomyces cerevisiae) based rat androgen receptor expression system to examine the cross-talk between different signalling pathways. we report here the synergistic modulation of androgen regulated transcriptional activation of beta-galactosidase reporter activity by the activators of protein kinase-a, like forskolin and 8-bromo-cyclic amp. a similar ligand-dependent enhancement of reporter activity compared to a dht treated control has been noticed with okadaic acid ...	1999	10024442
identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery.	we have identified a approximately 140 amino acid domain that is shared by a variety of proteins in budding and fission yeast, nematode, rat, mouse, frog, oat, and man. typically, this domain is located within 20 residues of the n-terminus of the various proteins. the percent identity among the domains in the 12 proteins ranges from 42 to 93%, with 16 absolutely conserved residues: n-x(11-13)-v-x2-a-t-x(34-36)-r-x(7-8)-w-r-x3-k-x12-g-x-e-x15 -l-x11-12-d-x-g-r-x11-d-x7-r. even though these protei ...	1999	10048338
molecular cloning and functional characterization of rat delta-6 fatty acid desaturase.	mammalian cdna fragments putatively encoding amino acid sequences characteristic of the fatty acid desaturase were obtained using expressed sequence tag (est) sequence informations. these fragments were subsequently used to screen a rat liver cdna library, yielding a 1573-bp clone. expression of dna fragment containing either of two possible open reading frames (nucleotide numbers 97-1431 and 148-1431) of the isolated clone in yeast led to the accumulation of gamma-linolenic acid in the presence ...	1999	10049752
a schizosaccharomyces pombe gene, ksg1, that shows structural homology to the human phosphoinositide-dependent protein kinase pdk1, is essential for growth, mating and sporulation.	fission yeast (schizosaccharomyces pombe) requires inositol for growth, mating and sporulation. to define putative genes that are involved in the processing and transduction of the inositol signal, mutants that are temperature sensitive for growth and sporulation were selected on a medium containing non-limiting amounts of inositol. two such mutants (ksg1-208 and ksg1-358) were analyzed, which are impaired in mating and sporulation at 30 degrees c and undergo growth arrest in the g2 phase of the ...	1999	10071224
opposite behaviors of reactive metabolites of tienilic acid and its isomer toward liver proteins: use of specific anti-tienilic acid-protein adduct antibodies and the possible relationship with different hepatotoxic effects of the two compounds.	tienilic acid (ta) is responsible for an immune-mediated drug-induced hepatitis in humans, while its isomer (tai) triggers a direct hepatitis in rats. in this study, we describe an immunological approach developed for studying the specificity of the covalent binding of these two compounds. for this purpose, two different coupling strategies were used to obtain ta-carrier protein conjugates. in the first strategy, the drug was linked through its carboxylic acid function to amine residues of carri ...	1999	10077492
notch signaling imposes two distinct blocks in the differentiation of c2c12 myoblasts.	notch signal transduction regulates expression of downstream genes through the activation of the dna-binding protein su(h)/cbf1. in drosophila most of notch signaling requires su(h); however, some notch-dependent processes occur in the absence of su(h) suggesting that notch signaling does not always involve activation of this factor. using constitutively active forms of notch lacking cbf1-interacting sequences we identified a notch signaling pathway that inhibits myogenic differentiation of c2c1 ...	1999	10079231
molecular cloning of clc chloride channels in oreochromis mossambicus and their functional complementation of yeast clc gene mutant.	we have cloned two members of the clc chloride channel family (omclc-3 and omclc-5) from gill cdna libraries of the euryhaline tilapia oreochromis mosammbicus. at the amino acid level, omclc-3 is 90.5% identical to rat clc-3 and omclc-5 is 79.2% identical to rat clc-5. ribonuclease protection assay revealed that omclc-5 was mainly expressed in the gill, kidney, and intestine in both freshwater- (fw) and seawater- (sw) adapted tilapia. although the mrna of omclc-3 was broadly expressed in tissues ...	1999	10082675
a conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates nup98 and the novel nucleoporin, nup96.	the mammalian nuclear pore complex (npc) is comprised of approximately 50 unique proteins, collectively known as nucleoporins. through fractionation of rat liver nuclei, we have isolated >30 potentially novel nucleoporins and have begun a systematic characterization of these proteins. here, we present the characterization of nup96, a novel nucleoporin with a predicted molecular mass of 96 kd. nup96 is generated through an unusual biogenesis pathway that involves synthesis of a 186-kd precursor p ...	1999	10087256
preparation of yac end fragments from the whitehead/mit mouse yac library prml vectors.		1999	10090972
delta 9-fatty acid desaturase from arachidonic acid-producing fungus. unique gene sequence and its heterologous expression in a fungus, aspergillus.	based on the sequence information for delta 9-desaturase genes (from rat, mouse and yeast), which are involved in the desaturation of palmitic acid and stearic acid to palmitoleic acid and oleic acid, respectively, the corresponding cdna and genomic gene were cloned from the fungal strain, mortierella alpina 1s-4, which industrially produces arachidonic acid. there was a cytochrome b5-like domain linked to the carboxyl terminus of this mortierella desaturase, as also seen in the yeast delta 9-de ...	1999	10091601
primary structure and biochemical characterization of yeast gtpase-activating proteins with substrate preference for the transport gtpase ypt7p.	small gtpases of the ypt/rab family are regulators of vesicular protein trafficking in exo-and endocytosis. gtpase-activating proteins (gap) play an important role as down regulators of gtpases. we here report the molecular cloning of a novel gap-encoding gene (gyp7, for gap for ypt7) by high expression from a saccharomyces cerevisiae genomic library. the gyp7 gene encodes a hydrophilic protein with a molecular mass of 87 kda. comparison of its primary sequence with that of the three other known ...	1999	10091609
high-level expressing yac vector for transgenic animal bioreactors.	the position effect is one major problem in the production of transgenic animals as mammary gland bioreactors. in the present study, we introduced the human growth hormone (hgh) gene into 210-kb human alpha-lactalbumin position-independent yac vectors using homologous recombination and produced transgenic rats via microinjection of yac dna into rat embryos. the efficiency of producing transgenic rats with the yac vector dna was the same as that using plasmid constructs. all analyzed transgenic r ...	1999	10092121
pax6 and pdx1 form a functional complex on the rat somatostatin gene upstream enhancer.	the somatostatin upstream enhancer (sms-ue) is a highly complex enhancer element. the distal a-element contains overlapping pdx1 and pbx binding sites. however, a point mutation in the a-element that abolishes both pdxl and pbx binding does not impair promoter activity. in contrast, a point mutation that selectively eliminates pdx1 binding to a proximal b-element reduces the promoter activity. the b-element completely overlaps with a pax6 binding site, the c-element. a point mutation in the c-el ...	1999	10094480
identification of nsf as a beta-arrestin1-binding protein. implications for beta2-adrenergic receptor regulation.	previous studies have demonstrated that beta-arrestin1 serves to target g protein-coupled receptors for internalization via clathrin-coated pits and that its endocytic function is regulated by dephosphorylation at the plasma membrane. using the yeast two-hybrid system, we have identified a novel beta-arrestin1-binding protein, nsf (n-ethylmaleimide-sensitive fusion protein), an atpase essential for many intracellular transport reactions. we demonstrate that purified recombinant beta-arrestin1 an ...	1999	10196135
[effect of biologically active food additives containing autolysate of baker's yeast and spirulina on intestinal permeability in an experiment].	influence of bioactive food supplements (bfa) intake on intestinal barrier permeability to macromolecules of polyethylene glycol 4000 was studied in rats with intestinal anaphylaxis and after external gamma-irradiation. bfa studied included autolysed baker's yeast ("vitasil") and edible algae spirulina platensis. intake of complex additive vitasil + spirulina resulted in significant diminution of permeability before irradiation and its partial normalization (24% decrease) after irradiation. spir ...	1999	10198958
differentially expressed genes in c6.9 glioma cells during vitamin d-induced cell death program.	c6.9 rat glioma cells undergo a cell death program when exposed to 1, 25-dihydroxyvitamin d3 (1,25-d3). as a global analytical approach, we have investigated gene expression in c6.9 engaged in this cell death program using differential screening of a rat brain cdna library with probes derived from control and 1,25-d3-treated cells. using this methodology we report the isolation of 61 differentially expressed cdnas. forty-seven cdnas correspond to genes already characterized in rat cells or tissu ...	1998	10200452
trematode and monogenean rrna its2 secondary structures support a four-domain model.	the secondary structure of rrna internal transcribed spacer 2 is important in the process of ribosomal biogenesis. trematode its sequences are poorly conserved and difficult to align for phylogenetic comparisons above a family level. if a conserved secondary structure can be identified, it can be used to guide primary sequence alignments. its2 sequences from 39 species were compared. these species span four orders of trematodes (echinostomiformes, plagiorchiformes, strigeiformes, and paramphisto ...	1998	9767686
a novel phosphatase regulating neurite extension on cns inhibitors.	the inability of injured axons to regenerate in the adult mammalian central nervous system is thought to be in part due to inhibitory molecules synthesized by oligodendrocytes and present in myelin. we describe the cloning of a cdna encoding a novel neuronal protein, named nerpp-2c, which is distantly related to protein phosphatase 2c and plays a role in the inhibitory response pathway to myelin inhibitors. nerpp-2c is expressed in neuronal cell lines and in rat brain. expression in rat is detec ...	1998	9770338
novel mutants of nab corepressors enhance activation by egr transactivators.	the ngfi-a binding corepressors nab1 and nab2 interact with a conserved domain (r1 domain) within the egr1/ngfi-a and egr2/krox20 transactivators, and repress the transcription of egr target promoters. using a novel adaptation of the yeast two-hybrid screen, we have identified several point mutations in nab corepressors that interfere with their ability to bind to the egr1 r1 domain. surprisingly, nab proteins bearing some of these mutations increased egr1 activity dramatically. the mechanism un ...	1998	9774344
role of hepatic fatty acid:coenzyme a ligases in the metabolism of xenobiotic carboxylic acids.	1. formation of acyl-coenzymes (co)a occurs as an obligatory step in the metabolism of a variety of endogenous substrates, including fatty acids. the reaction is catalysed by atp-dependent acid:coa ligases (ec 6.2.1.1-2.1.3; amp forming), classified on the basis of their ability to conjugate saturated fatty acids of differing chain lengths, short (c2-c4), medium (c4-c12) and long (c10-c22). the enzymes are located in various cell compartments (cytosol, smooth endoplasmic reticulum, mitochondria ...	1998	9784915
identification of a novel highly conserved gene in the centromeric part of the major histocompatibility complex.	a novel highly conserved gene designated sacm2l alias are1) has been identified and fine mapped in the centromeric part of the major histocompatibility complex in rat, human, and mouse. sacm2l is closely linked to the ribosomal protein s18 gene rps18 with a distance of about 450 bp between the respective translational start points. numerous sacm2l-homologous est sequences can be identified in the database. northern blot experiments of rat sacm2l revealed a transcript of 3 kb in each organ tested ...	1998	9790748
binding of myosin essential light chain to the cytoskeleton-associated protein iqgap1.	the 190 kd human iqgap1 protein, by virtue of its n-terminal calponin-homology domain, is found associated with the actin cytoskeleton, and is capable of cross-linking actin filaments. iqgap1 complexes with several proteins, including the rho family gtpases cdc42 and rac, as well as calmodulin. it was previously noted that one of the iq motifs of iqgap1 displays significant similarity to a myosin heavy chain iq motif responsible for binding the calmodulin-related myosin essential light chain (el ...	1998	9790945
the use of the yeast two hybrid system to evaluate erbb-3 interactions with sh2 domain containing proteins.	several mutations in the tyrosine kinase domain of erbb-3 have been postulated to render this enzyme catalytically inactive. to test which amino acid mutations in erbb-3 might be critical for kinase inactivation, we used a yeast two hybrid assay of protein-protein interaction. we monitored restoration of erbb-3 kinase activity by investigating the ability of wild type or mutant erbb-3 to associate with the sh2 containing proteins syp and phosphatidyl-inositol-3-kinase (pi3k). our results demonst ...	1998	9791008
valine 904, tyrosine 898, and cysteine 908 in na,k-atpase alpha subunits are important for assembly with beta subunits.	a 26-amino acid sequence in an extracellular loop of the na,k-atpase alpha subunit between membrane-spanning segments 7 and 8 has been shown to bind to the beta subunit of na,k-atpase and to promote alphabeta assembly (lemas, m. v., hamrick, m., takeyasu, k., and fambrough, d. m. (1994) j. biol. chem. 269, 8255-8259) when this 26-amino acid sequence of the rat na,k-atpase alpha3 subunit was replaced by the corresponding sequence of the rat gastric h,k-atpase alpha subunit, the chimeric alpha sub ...	1998	9792642
association of hap1 isoforms with a unique cytoplasmic structure.	hap1 is a neural protein and interacts with the huntington's disease protein huntingtin. there are at least two hap1 isoforms, hap1-a and hap1-b, which have different c-terminal amino acid sequences. here we report that both hap1 isoforms associate with a unique cytoplasmic structure in neurons of rat brain. the hap1-immunoreactive structure appears as an inclusion that is an oval mass of electron-dense material, 0.5-3 microm in diameter, containing many curvilinear or ring-shaped segments, and ...	1998	9798945
metabolism of benzo[a]pyrene and benzo[a]pyrene-7,8-diol by human cytochrome p450 1b1.	benzo[a]pyrene (b[a]p), a ubiquitous environmental, tobacco and dietary carcinogen, has been implicated in human cancer etiology. the role of human cytochrome p450 1b1 in the metabolism of b[a]p is poorly understood. using microsomal preparations of human p450 1a1, 1a2 and 1b1 expressed in baculovirus-infected insect cells, as well as human and rat p450 1b1 expressed in yeast, we have determined the metabolism of b[a]p, with and without the addition of exogenous epoxide hydrolase, and b[a]p-7,8- ...	1998	9806168
recently identified molecular aspects of intestinal iron absorption.	gene mapping techniques to identify gene mutations in humans and animals with phenotypic abnormalities in iron metabolism are providing important insights into the probable molecular mediators of intestinal iron absorption. positional gene cloning in humans with hereditary hemochromatosis has identified a mutation in a novel major histocompatibility complex (mhc) gene called hfe that is likely to be involved in regulating intestinal iron absorption. in addition, recent observations based on posi ...	1998	9808632
binding of dynein light chain (pin) to neuronal nitric oxide synthase in the absence of inhibition.	pin, an 89-amino-acid polypeptide found in a rat hippocampal cdna library using the yeast two-hybrid system and various neuronal nitric oxide synthase (nnos) fragments as bait, was reported to be an inhibitor of nnos (science 274, 774-778, 1996). pin reportedly inhibited nnos selectively and did not interact with either the endothelial or inducible nitric oxide synthase isoforms. inhibition was attributed to the ability of pin to dissociate the catalytically active nnos homodimer. pin is a dynei ...	1998	9808772
hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms. evidence for a transcriptional activation function of hpttg.	we have isolated a human cdna clone encoding a novel protein of 22 kda that is a human counterpart of the rat oncoprotein pttg. we show that the corresponding gene (hpttg) is overexpressed in jurkat cells (a human t lymphoma cell line) and in samples from patients with different kinds of hematopoietic malignancies. analysis of the sequence showed that hpttg has an amino-terminal basic domain and a carboxyl-terminal acidic domain, and that it is a proline-rich protein with several putative sh3-bi ...	1998	9811450
human carbon catabolite repressor protein (ccr4)-associative factor 1: cloning, expression and characterization of its interaction with the b-cell translocation protein btg1.	the human btg1 protein is thought to be a potential tumour suppressor because its overexpression inhibits nih 3t3 cell proliferation. however, little is known about how btg1 exerts its anti-proliferative activity. in this study, we used the yeast 'two-hybrid' system to screen for interacting protein partners and identified human carbon catabolite repressor protein (ccr4)-associative factor 1 (hcaf-1), a homologue of mouse caf-1 (mcaf-1) and saccharomyces cerevisiae ycaf-1/pop2. in vitro the hcaf ...	1998	9820826
expression of active human tissue-type plasminogen activator in escherichia coli.	the formation of native disulfide bonds in complex eukaryotic proteins expressed in escherichia coli is extremely inefficient. tissue plasminogen activator (tpa) is a very important thrombolytic agent with 17 disulfides, and despite numerous attempts, its expression in an active form in bacteria has not been reported. to achieve the production of active tpa in e. coli, we have investigated the effect of cooverexpressing native (dsba and dsbc) or heterologous (rat and yeast protein disulfide isom ...	1998	9835579
cloning of a third member of the d52 gene family indicates alternative coding sequence usage in d52-like transcripts.	d52 proteins are emerging as signalling molecules which may be regulators of cell proliferation. having previously reported the existence of the human d52 gene family, comprising the hd52 and hd53 genes expressed in human breast carcinoma, we report the identification of a novel human gene hd54 (tpd52l2), which represents a third d52 gene family member. in situ mapping placed the hd54 gene on human chromosome 20q13.2-q13.3, a localization distinct from those of both hd52 and hd53 genes. the iden ...	1998	9838088
enhanced clearance of a multiple antibiotic resistant staphylococcus aureus in rats treated with pgg-glucan is associated with increased leukocyte counts and increased neutrophil oxidative burst activity.	pgg-glucan [betafectin], a highly purified soluble beta-(1-6)-branched beta-(1 3)-linked glucan isolated from saccharomyces cerevisiae, has broad in vitro and in vivo anti-infective activities unrelated to cytokine induction. here we present in vivo results on the anti-infective activity of pgg-glucan against a multiple antibiotic resistant staphylococcus aureus. pgg-glucan (0.25-4 mg/kg) was administered intramuscularly to male wistar rats 48 h, 24 h, and 4 h before and 4 h after intraperitonea ...	1998	9848393
cloning and characterization of a novel mammalian pp2c isozyme.	pp2c is a structurally diversified protein phosphatase family with a wide range of functions in cellular signal transduction. a novel pp2c subtype, designated pp2cdelta, was identified from a rat cdna clone, which encodes a protein of 392 amino acid residues. while pp2cdelta shares approximately 30% sequence identity in its catalytic domain with the mammalian pp2c, it lacks a 90-residue carboxyl-terminal sequence conserved in mammalian pp2c. northern blot analysis showed that pp2cdelta is widely ...	1998	9857069
identification and characterization of stk12/aik2: a human gene related to aurora of drosophila and yeast ipl1.	mutations in aurora of drosophila and related saccharomyces cerevisiae ipl1 protein kinases are known to cause abnormal chromosome segregation. we earlier isolated a cdna encoding a novel human protein kinase aik which shares high amino acid identity with the aurora/ipl1 protein kinase family. in the present study, a second human cdna highly homologous to aurora/ipl1 (aik2) was identified and the nucleotide sequence was determined (gene symbol stk12). the c-terminal kinase domain of the stk12 en ...	1998	9858806
mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2.	mint 1 and 2 are proteins that bind to munc18-1, an essential component of the synaptic vesicle fusion machinery, and are detectably expressed only in neurons [okamoto and südhof, j. biol. chem. 272, 31459-31464 (1997)]. mint 1 and 2 are composed of a variable n-terminal region that includes a conserved munc18-1-binding site, and a constant c-terminal region that contains one ptb and two pdz domains. we have now identified a third mint isoform, mint 3. similar to mint 1 and 2, the c-terminal hal ...	1998	9860131
the hsp70 gene family in pneumocystis carinii: molecular and phylogenetic characterization of cytoplasmic members.	pneumocystis carinii, a major opportunistic lung pathogen of aids patients, is found in a number of mammals and is proposed to be a member of the fungi. in this work, several members of the highly conserved hsp70 multigene family were characterized from rat-derived p. carinii. previously, we reported characterization of the er resident hsp70 homolog known as bip from prototype (p.c. carinii) and variant (p. c. rattus) strains of the organism. we report here, from p. c. carinii, characterization ...	1998	9864849
proteins involved in membrane transport between the er and the golgi apparatus: 21 putative plant homologues revealed by dbest searching.	numerous proteins have been identified in yeast and mammalian cells which are involved in trafficking between the endoplasmic reticulum and the golgi apparatus. a great number of partial cdna sequences now available from the two major plant model species, arabidopsis thaliana and oryza sativa, makes it possible to identify putative plant homologues of known genes/proteins from non-plant species. the authors used this approach to screen the database of expressed sequence tags (dbest) in order to ...	1998	9878102
characterization of the mouse src homology 3 domain gene sh3d2c on chr 7 demonstrates coexpression with huntingtin in the brain and identifies the processed pseudogene sh3d2c-ps1 on chr 2.	formation of intracellular protein complexes is often mediated by src homology 3 domain-containing proteins interacting with proline-rich target sequences on other proteins. the sh3d2c gene or its rat/human orthologs have been implicated in synaptic vesicle recycling due to interaction with dynamin i and synaptojanin in nerve terminals. in a yeast two-hybrid system, association with a huntingtin fragment containing an elongated stretch of polyglutamines was observed recently. by genetic mapping ...	1998	9878254
identification of ce-af-6, a novel caenorhabditis elegans protein, as a putative ras effector.	mammalian ras proteins associate with multiple effectors, including raf, ral guanine nucleotide dissociation stimulator, phosphoinositide 3-kinase and af-6. in the nematode caenorhabditis elegans, lin-45/raf has been identified genetically as an effector of let-60/ras. to search for other effectors in c. elegans, we carried out a yeast two-hybrid screening for let-60-associating proteins. the screening identified a novel protein, designated ce-af-6, which exhibited a strong structural homology w ...	1998	9931431
cloning of human p55 gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-i receptor.	we have used the yeast two-hybrid system to identify proteins that interact with the intracellular domain of the insulin-like growth factor-i receptor (igfir). in a search of a human fetal brain library we identified a cdna encoding a protein that is the human homologue of mouse p55pik, a regulatory subunit of phosphatidylinositol 3-kinase (hp55 gamma). the hp55 gamma protein interacts strongly with the activated igfir but not with the kinase-negative mutant receptor. hp55 gamma also interacts w ...	1998	9524259
the carboxy-terminal domain of hsc70 provides binding sites for a distinct set of chaperone cofactors.	the modulation of the chaperone activity of the heat shock cognate hsc70 protein in mammalian cells involves cooperation with chaperone cofactors, such as hsp40; bag-1; the hsc70-interacting protein, hip; and the hsc70-hsp90-organizing protein, hop. by employing the yeast two-hybrid system and in vitro interaction assays, we have provided insight into the structural basis that underlies hsc70's cooperation with different cofactors. the carboxy-terminal domain of hsc70, previously shown to form a ...	1998	9528774
cloning, differential regulation and tissue distribution of alternatively spliced isoforms of adp-ribosylation-factor-dependent phospholipase d from rat liver.	an alternatively spliced isoform of adp-ribosylation-factor-dependent phospholipase d (pld1) was previously shown to occur in rat c6 cells [yoshimura, nakashima, ohguchi, sakai, shinoda, sakai and nozawa (1996) biochem. biophys. res. commun. 225, 494-499] and human hela cells [hammond, jenco, nakashima, cadwallader, gu, cook, nozawa, prestwich, frohman and morris (1997) j. biol. chem. 272, 3860-3868]. however, its complete sequence and the enzymological difference between the two pld1 isoforms w ...	1998	9445394
isozyme function of n-alkane-inducible cytochromes p450 in candida maltosa revealed by sequential gene disruption.	an n-alkane-assimilating yeast candida maltosa contains multiple n-alkane-inducible forms of cytochromes p450 (p450alk), which can be assumed to catalyze terminal hydroxylation of n-alkanes in the assimilation pathway. eight structurally related p450alk genes have been identified. in the present study, the function of four major isoforms of p450alk (encoded by alk1, alk2, alk3, and alk5 genes) was investigated by sequential gene disruption. auxotrophic markers used for the selection of disrupted ...	1998	9461581
further studies on the coupling of mitochondrially bound hexokinase to intramitochondrially compartmented atp, generated by oxidative phosphorylation.	hexokinase, bound to nonphosphorylating rat brain mitochondria, exhibits michaelis-menten kinetic behavior, with an apparent k(m) for atp of 0.44 +/- 0.08 mm. after initiation of oxidative phosphorylation, a steady-state rate of glc phosphorylation is maintained despite the fact that extramitochondrial [atp] continues to increase but remains well below saturating levels (i.e., < 0.4 mm). this independence from extramitochondrial [atp] is taken to indicate that hexokinase is not utilizing extrami ...	1998	9466827
rodent y chromosome tspy gene is functional in rat and non-functional in mouse.	recombination is believed to prevent genetic deterioration in sexual populations because it allows conservation of functional genotypes by removing deleterious mutations. moreover, evidence that non-recombining segments of a genome deteriorate is provided by genetic experiments in drosophila and yeast. y chromosomes generally do not recombine along most of their length, and thus y chromosome genes, despite having been selectively maintained for their function, could be lost from the genome. here ...	1998	9467017
dissection of the translocation and chaperoning functions of yeast bip/kar2p in vivo.	we used the rat nerve growth factor receptor ectodomain (ngfre) and escherichia coli ss-lactamase to dissect the functions of saccharomyces cerevisiae bip/kar2p in vivo. both were fused to the hsp150delta-polypeptide, which promotes proper folding of heterologous proteins which otherwise are misfolded in the yeast er. hsp150delta-ngfre and hsp150delta-beta-lactamase acquired disulfides and were properly folded and oncreted to the culture medium. when disulfide formation was prevented by incubati ...	1998	9472003
the box h + aca snornas carry cbf5p, the putative rrna pseudouridine synthase.	many or all of the sites of pseudouridine (psi) formation in eukaryotic rrna are selected by site-specific base-pairing with members of the box h + aca class of small nucleolar rnas (snornas). database searches previously identified strong homology between the rat nucleolar protein nap57p, its yeast homolog cbf5p, and the escherichia coli psi synthase trub/p35. we therefore tested whether cbf5p is required for synthesis of psi in the yeast rrna. after genetic depletion of cbf5p, formation of psi ...	1998	9472021
neurofilament (nf) assembly; divergent characteristics of human and rodent nf-l subunits.	previous studies have shown that rodent neurofilaments (nf) are obligate heteropolymers requiring nf-l plus either nf-m or nf-h for filament formation. we have assessed the competence of human nf-l and nf-m to assemble and find that unlike rat nf-l, human nf-l is capable of self-assembly. however, human nf-m cannot form homopolymers and requires the presence of nf-l for incorporation into filaments. to investigate the stage at which filament formation is blocked, the rod domains or the full-leng ...	1998	9478962
functional comparison of the yeast scerv1 and scerv2 genes.	the yeast scerv1 gene is the first representative of a new emerging gene family. its gene product is essential for the yeast cell and is involved in the biogenesis of mitochondria and the regulation of the cell cycle. recently the general importance of the gene for the eukaryotic cell was shown by the identification of a structural and functional human homologue. the homologous mammalian alr (augmenter of liver regeneration) genes from man, mouse and rat are important for different developmental ...	1998	9483805
identification and characterization of an endo/exonuclease in pneumocystis carinii that is inhibited by dicationic diarylfurans with efficacy against pneumocystis pneumonia.	dicationic diarylfurans and dicationic carbazoles are under development as therapeutic agents against opportunistic infections. while their ability to bind to the minor groove of dna has been established, the complete mechanism of action has not. we demonstrate here that an effective diarylfuran, 2,5-bis[4-(n-isopropylguanyl)phenyl]furan, inhibits an endo/exonuclease activity present in pneumocystis carinii, cryptococcus neoformans, candida albicans, and saccharomyces cerevisiae. this activity w ...	1998	9495040
heterologous expression and purification of recombinant rolipram-sensitive cyclic amp-specific phosphodiesterases.	with the cloning of cdnas coding for the different phosphodiesterase 4 (pde4) isoenzymes present in mammals, homogeneous preparations of these forms have become readily available. this strategy has greatly facilitated the understanding of the properties of the myriad of isoforms derived from the four pde4 genes found in mammals, and has opened a new avenue to develop inhibitors with a different degree of selectivity for each isoform. here we describe the strategies and methods used to express pd ...	1998	9500858
cloning and sequencing of cdna encoding the diazepam binding inhibitor (dbi).	the anti-morphine peptide ppc from dog brain has an inhibitory effect on morphine analgesia. it is highly homologous to dbi. the analgesic effect of bovine dbi was measured, showing the potentiation effect on morphine analgesia. in order to study the relationship between ppc and dbi, cdna library derived from dog liver had been screened using partial gene of rat dbi as a probe. a positive clone was obtained. the entire sequence of the insert of this positive clone provided complete sequence of a ...	1998	12168001