Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| detection of viral genome in non-neural tissues of cattle experimentally infected with bovine herpesvirus 1. | to obtain further information on the latent infection with bovine herpesvirus 1 (bhv-1) in the natural host, we examined the nasal secretions and various tissues of experimentally infected calves using virus isolation, polymerase chain reaction (pcr), and immuno-pcr/antigen (immuno-pcr/ag) assays. in the nasal secretions, viral dna was detected in samples with virus isolation titers of 10(4.3) tcid50 or more by pcr. on the same samples, immuno-pcr/ag remained positive up to day 19 p.i. the last ... | 1996 | 8997877 |
| pathogenesis of ruminant herpesvirus infections. | ruminants are hosts for members of both alpha- and gamma-herpesvirinae. a wide range of disease syndromes is associated with infections by these agents. the associated diseases reflect the biological nature of the causative viruses. clinically, the symptoms may be mild and localized or include severe generalized disease, leading eventually to death. much knowledge has been gained concerning the pathogenesis of some alpha-herpesviruses. initially, these viruses replicate in epithelial cells at th ... | 1996 | 9010994 |
| molecular virology of ruminant herpesviruses. | molecular virology has served to establish bovine herpesvirus 1 (bhv-1) as the prototype member of ruminant herpesviruses. based on the genomic sequence of the virus, we aim to identify and characterize virus-specified components, to explain their concerted action, and to predict how the chain of events during the lytic and latent phases of the viral life cycle may be interrupted. the nucleotide sequence of the bhv-1 genome (136 kb) has just been completed by international cooperation (july 1995 ... | 1996 | 9010995 |
| immunology of bovine herpesvirus 1 infection. | immune responses to bovine herpesvirus 1 (bhv-1) have been studied following exposure of animals to virulent virus, conventional live or killed vaccines, genetically engineered live virus vaccines, subunit vaccines and, more recently, following immunization with plasmids encoding putative protective antigens. in all cases reported to date, exposure to bhv-1 or its glycoproteins induced specific responses to the virus which are capable of neutralizing virus and killing virus infected cells. these ... | 1996 | 9010996 |
| advances in the development and evaluation of bovine herpesvirus 1 vaccines. | this review deals with conventional and modern bovine herpesvirus 1 (bhv1) vaccines. conventional vaccines are widely used to prevent clinical signs of infectious bovine rhinotracheitis. the use of conventional vaccines, however, does not appear to have resulted in reduction of the prevalence of infection. novel bhv1 marker vaccines comprise either mutants with a deletion in one of the non-essential genes, or subunit vaccines that contain one or more glycoproteins. these marker vaccines can be u ... | 1996 | 9010997 |
| gene contents in a 31-kb segment at the left genome end of bovine herpesvirus-1. | we report the nucleotide sequence of a 31-kb segment at the left genome end of bovine herpesvirus-1 (bhv-1) and show that it comprises 19 different open reading frames (orfs), including seven which have been described previously (circ, dutpase, ul49.5, alpha tif, vp8, glycoprotein c, and ribonucleotide reductase small subunit). the new sequence resulted in a correction at the c-terminus of glycoprotein c. all 19 orfs exhibited strong amino acid sequence homology to the gene products of other alp ... | 1996 | 9010999 |
| structural and functional analysis of bovine herpesvirus 1 minor glycoproteins. | this paper focuses on the structure and functions of bovine herpesvirus 1 minor glycoproteins gh, ge, gg and gp42. it reviews the progress which has been made in their identification and characterization, in the study of their temporal expression and processing in infected cells, and finally in the understanding of their biological activities. in addition, aspects discussed include a comparison with two other alphaherpesviruses, namely herpes simplex virus and pseudorabies virus. | 1996 | 9011001 |
| persistence of antibodies against bovine herpesvirus 1 and virus reactivation two to three years after infection. | to study the long-term persistence of bovine herpesvirus 1 (bhv1) specific antibodies in cattle, serum samples were collected regularly over a period of two to three years after infection. the sera were titrated in a bhv1 neutralizing antibody test, a glycoprotein gb blocking elisa and a glycoprotein ge blocking elisa. sera collected after infection were positive in all tests and the titres declined only minimally during the investigation period. two to three years after infection, four animals ... | 1996 | 9011002 |
| the role of glycoproteins gc, ge, gi, and gg in the induction of cell-mediated immune responses to bovine herpesvirus 1. | mutant viruses with deletions in genes encoding non-essential glycoproteins are considered as promising bovine herpesvirus 1 (bhv1) vaccine candidates. the present study compared the influence of various gene deletions (gc, ge, gi, gg) on the induction of cell-mediated immune responses against the virus. the highest bhv1 specific lymphoproliferative response was observed in the group of calves inoculated with the gc- mutant. however, in all groups of inoculated calves, limiting dilution analysis ... | 1996 | 9011004 |
| assessment of the cell-mediated immunity in cattle infection after bovine herpesvirus 4 infection, using an in vitro antigen-specific interferon-gamma assay. | the cell-mediated immunity (cmi) following bovine herpesvirus 4 (bhv4) infection has been poorly investigated in cattle. the in vivo response measured by a delayed type of hypersensitivity (dth) assay has been reported to be positive in only few animals showing serological evidences of bhv4 infection. we have investigated the cmi following bhv4 infection by an in vitro antigen-specific interferon gamma (ifn-gamma) release assay, as an indicator of an actively acquired immunity to bhv4. our preli ... | 1996 | 9011005 |
| antibody isotype responses to experimental infection with bovine herpesvirus 1 in calves with colostrally derived antibody. | calves, both positive and negative for maternal antibody to bovine herpesvirus 1 (bhv-1), were infected with bhv-1 and their serum antibody isotype responses were measured post-infection. in the case of maternal antibody negative calves there was a classical humoral response to the virus with an early igm peak followed by igg1 and igg2 responses. in maternal antibody positive calves, although infection was established, no active antibody response was detected in serum except for a transient igm ... | 1996 | 9011006 |
| a european inter-laboratory trial to evaluate the reliability of serological diagnosis of bovine herpesvirus 1 infections. | the detection of cattle latently infected with bovine herpesvirus 1 (bhv1) is of importance in control programs and in international trade activities. therefore, tests to detect specific antibodies in serum must be highly sensitive. to evaluate the reliability of serological diagnosis of bhv1 infections in europe, seventeen laboratories in 15 european countries were asked to determine bhv1-specific antibodies in a panel of bovine serum samples using the serological tests available in their labor ... | 1996 | 9011007 |
| the use of a polymerase chain reaction assay for the detection of bovine herpesvirus 1 in semen during a natural outbreak of infectious bovine rhinotracheitis. | nasal swabs from two bulls at an artificial insemination (ai) station were submitted to our laboratory. the animals showed clinical signs of infectious bovine rhinotracheitis (ibr), although the station was supposedly free of bovine herpesvirus 1 (bhv1). dna of bhv1 was detected using a polymerase chain reaction (pcr). subsequently nasal swabs from 100 animals that could have been in contact were submitted. bhv1 dna was detected in swabs from 23 animals. using the pcr, bhv1 could only be detecte ... | 1996 | 9011008 |
| population dynamics of bovine herpesvirus 1 infection in a dairy herd. | an induced outbreak of a bovine herpesvirus 1 (bhv1) infection in a dairy herd is described. the outbreak was induced by injecting three bhv1 seropositive cows with dexamethasone. within 7 weeks all seronegative cows had seroconverted. also some seropositive animals showed a significant increase in serum antibody titre. using these data, parameters of population dynamics such as r0, the basic reproduction ratio, could be estimated. the basic reproduction ratio is a threshold value describing inf ... | 1996 | 9011009 |
| a ge deleted infectious bovine rhinotracheitis marker vaccine for use in improved bovine herpesvirus 1 control programs. | based on a glycoprotein e (ge) deleted bovine herpesvirus 1 (bhv1) strain (kaashoek et al., 1994) a killed virus as well as a modified live virus marker vaccine have been developed that allow differentiation between immunized and bhv1 infected cattle. safety and efficacy of both vaccines were tested extensively following the current european union (eu) requirements for the development of bovine vaccines. the minimum vaccine dose, vaccination regimen, route of administration and duration of immun ... | 1996 | 9011010 |
| early immunity induced by a live ge-negative bovine herpesvirus 1 marker vaccine. | we studied the early immunity induced by a live glycoprotein e (ge) negative bovine herpesvirus 1 (bhv1) marker vaccine. three groups of specific-pathogen-free calves were either not vaccinated, or vaccinated two days or two hours before the introduction of a calf that was intranasally infected with wild-type bhv1 the day before. we quantified the shedding of ge-negative vaccine virus and of wild-type virus, using a double-staining immunoassay. in calves vaccinated two hours before the introduct ... | 1996 | 9011011 |
| development of a disease control programme based on the use of an inactivated vaccine against infectious bovine rhinotracheitis. | studies to investigate the efficacy of an inactivated vaccine against infectious bovine rhinotracheitis (ibr) suggest that this vaccine can prevent the in utero infection of calves from experimentally infected dams. in an experimental herd the inactivated vaccine induced a humoral immune response in both seropositive and seronegative cattle and, after subsequent intratracheal infection with ibr (bhv-1) virus, prevented development of symptoms in the cows and protected their fetuses against infec ... | 1996 | 9011012 |
| the ul2 open reading frame of bovine herpesvirus 1 encodes a uracil-dna glycosylase. | sequence analysis within the unique long segment of the bovine herpesvirus 1 (bhv-1) genome previously identified an open reading frame (orf), designated ul2, whose deduced polypeptide of 204 amino acids contained a consensus uracil-dna glycosylase (udgase) signature sequence. to determine whether the bhv-1 ul2 orf product has udgase activity, we positioned the ul2 sequence down-stream of the t7 promoter on the vector pet-28b(+) and expressed it in escherichia coli. upon induction with isopropyl ... | 1996 | 9013493 |
| effects of bhv-1 on pmn adhesion to bovine lung endothelial cells. | bovine herpes virus-1 (bhv-1) infection appears to decrease the rate of polymorphonuclear leukocyte (pmn) influx into the lung in response to the secondary invader, pasteurella haemolytica. it was postulated that bhv-1 may affect the rate of cellular infiltration by altering the function of the endothelium, thereby preventing pmn movement across the blood-tissue barrier. therefore, we decided to investigate the effect of bhv-1 on the ability of pmn to adhere to lung endothelial cells (lec). lec ... | 1996 | 9014307 |
| the effect of cobalt supplementation on the immune response in vitamin b12 deficient texel lambs. | the effect of cobalt supplementation on the immune reactivity in vitamin b12 deficient lambs was measured by comparing the humoral and cell-mediated immune responses against bovine herpes virus type 1 and mycobacterium paratuberculosis. in addition, faecal egg counts were performed after natural infection with gastrointestinal nematodes. the experiments were performed with registered texel twin lambs of the same sex. one lamb of each twin received three cobalt pellets divided over the grazing pe ... | 1996 | 9014314 |
| the bovine herpesvirus type 1 major tegument protein vp8 expressed in recombinant vaccinia virus does not induce significant immunity in mice. | we previously reported the characterization of the gene encoding the bovine herpesvirus type 1 (bhv-1) major tegument protein vp8. with the aim of defining the immunological properties of this protein, we constructed a recombinant vaccinia virus (vv-vp8) in which expression of the vp8 gene was regulated by the p7.5 early/late promoter. since the sequence of the vp8 gene contained a tttttnt motif known to serve as a transcription termination signal of vaccinia virus genes of the early class, a se ... | 1996 | 8725115 |
| immunogenicity of bovine herpesvirus 1 glycoprotein d in mice: effect of antigen form on the induction of cellular and humoral immune responses. | for the development of veterinary subunit vaccines, modifications to the antigen may be needed to make the production of these vaccines cost effective. to investigate the effect of antigen modifications on immune response, we used glycoprotein d, one of the major glycoproteins of bovine herpesvirus-1 (bhv-1), as a model antigen. we developed a mouse model to assess the immune response elicited by immunization with either a recombinant truncated (tgd) or the authentic full-length (gd) form of bhv ... | 1996 | 8733915 |
| demonstration of infectious bovine rhinotracheitis virus antigen by immunoperoxidase method in tissues of aborted bovine fetuses preserved for 25 years in paraffin blocks. | infectious bovine rhinotracheitis (ibr) virus antigen was demonstrated by the immunoperoxidase method in tissues of two aborted bovine fetuses, which had been stored for 25 years after fixing in formalin and embedding in paraffin. necrotic foci were detected in the liver, kidney, adrenal gland, and thymus of the fetuses. coincidenting with the distribution of the necrotic foci, ibr virus antigen was demonstrated by immunostaining. the present study proved the immunoperoxidase method in one of us ... | 1996 | 8741609 |
| glycoprotein h (gii/gp108) and glycoprotein l form a functional complex which plays a role in penetration, but not in attachment, of bovine herpesvirus 1. | the glycoproteins of bovine herpesvirus 1 (bhv-1) play important roles in the interactions between virions and target cells. a 108 kda glycoprotein, designated gii or gp 108, has been identified by two different panels of monoclonal antibodies. the gii- and gp 108-specific monoclonal antibodies were shown to react with the same protein, which was identified by n-terminal sequencing as the homologue of herpes simplex virus type 1 (hsv-1) gh. when bhv-1 gh was purified by immunoadsorbent chromatog ... | 1996 | 8757994 |
| identification of a 56 kda putative bovine herpesvirus 1 cellular receptor by anti-idiotype antibodies. | polyclonal anti-idiotype antibodies (anti-ids) to anti-bovine herpesvirus 1 (bhv-1) mabs blocked virus infection in cell cultures, indicating that they contain internal images of a viral attachment protein(s). in the present study anti-id (anti-83) of bhv-1 gb was used as a probe to detect the cellular receptor. anti-id specifically identified a 56 kda protein in radioimmunoprecipitation analysis (ripa) of madin-darby bovine kidney (mdbk) cell membranes suggesting the involvement of this cell su ... | 1996 | 8760439 |
| serological and virological studies on the infectious bovine rhinotracheitis in algeria. | clinical cases of infectious bovine rhinotracheitis and infectious pustular vulvovaginitis were observed within the cattle population in algeria. the isolation and identification of six bovine herpes virus type 1 strains from diseased cattle and the serological surveys undertaken in the central region of the country on 2948 sera revealed a 20.5% incidence of ibr infections in algeria. the dna restriction profiles of the isolated strains showed the existence of only the bhv1-2a subtype associated ... | 1996 | 8767771 |
| immunogenicity of infectious bovine rhinotracheitis virus (bhv-1) proteins integrated into iscoms or liposomes. | dynamics and persistence of antibody responses to immunization and reimmunization of rabbits with bhv-1 iscoms or bhv-1 liposomes were investigated for 20 weeks and compared with those induced by a commercial vaccine against infectious bovine rhinotracheitis (ibr) or free bhv-1 protein subunits. comparable levels of antibodies to bhv-1, determined by elisa, were found throughout the observation period in rabbits treated with bhv-1 iscoms or the commercial vaccine. the immunogenicity of bhv-1 lip ... | 1996 | 8774120 |
| the effect of molybdenum-induced copper deficiency on acute-phase protein concentrations, superoxide dismutase activity, leukocyte numbers, and lymphocyte proliferation in beef heifers inoculated with bovine herpesvirus-1. | this study was conducted to determine the effect of cu deficiency on acute-phase protein concentrations, superoxide dismutase activity, leukocyte numbers, and lymphocyte proliferation in heifers inoculated with live bovine herpesvirus-1 (bhv-1). hereford x angus heifers were allotted by weight and initial liver cu concentrations into molybdenum (mo)-supplemented (n = 6) or control (n = 6) groups. control heifers were fed a basal diet supplemented with cu-sulfate to achieve a dietary concentratio ... | 1996 | 8778102 |
| rapid detection of bovine herpesvirus 1 in the semen of infected bulls by a nested polymerase chain reaction assay. | a nested polymerase chain reaction (pcr) assay was developed for the detection of bovine herpesvirus 1 (bhv-1) in bovine semen and compared with the virus isolation method. when extended semen, commonly used in the bovine artificial insemination industry, was inoculated with bhv-1, the pcr assay detected bhv-1 dna in semen inoculated at 0.25-2.5 tcid50 per 0.5 ml. in contrast, the lower limit of detection for virus isolation was 250 tcid50 of bhv-1 inoculated in 0.5 ml of extended semen. these m ... | 1996 | 8785714 |
| serological responses in calves to vaccines against bovine respiratory syncytial, infectious bovine rhinotracheitis, bovine viral diarrhoea and parainfluenza-3 viruses. | the istituto superiore di sanità (iss), the national veterinary services laboratory in italy, is in charge of assessing the quality, safety and efficacy of veterinary vaccines before and after licensing. to evaluate the relative potency of several vaccines against bovine respiratory syncytial virus (brsv), infectious bovine rhinotracheitis virus (ibrv), bovine viral diarrhoea virus (bvdv) and parainfluenza-3 virus (pi3v), the serological responses in vaccinated calves were studied. vaccination w ... | 1996 | 8785944 |
| effects of b vitamin injections on plasma b vitamin concentrations of feed-restricted beef calves infected with bovine herpesvirus-1. | for nonruminants, stress and disease greatly increase requirements for vitamin b6, folic acid, pantothenic acid, and ascorbate. the effects of feed restriction, virus infection, and vitamin injections on plasma concentrations of b vitamins critical to the immune response were evaluated. twelve beef steer calves, 6 to 8 mo of age, were fed below maintenance for 17 d and deprived of food for 3 d during a 20-d period after weaning. they then were inoculated intranasally with live attenuated bovine ... | 1996 | 8791209 |
| effects of b vitamin injection on bovine herpesvirus-1 infection and immunity in feed-restricted beef calves. | because feed and water deprivation during marketing and transport of feedlot calves may reduce ruminal b vitamin synthesis at a time when calves are most susceptible to infectious agents, we studied the effect of b vitamin injections on infection and immunity in 12 6-mo-old beef steer calves (153 +/- 8 kg) that were weaned, limit-fed, and deprived of feed. six calves were injected with b vitamins and ascorbic acid every 48 h for 28 d starting 2 wk before virus inoculation. all calves were infect ... | 1996 | 8791210 |
| serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease. | the early stages of the host response to infectious agents include a number of physiologic changes, collectively known as the acute phase response. the acute phase response is comprised of reactions localized at the site of infection, as well as the initiation of systemic responses, which include a rapid increase in the serum concentration of some proteins, known as acute phase proteins (app). using polyacrylamide gel electrophoresis, we detected two app of approximately 22 and 37 kda molecular ... | 1996 | 8792565 |
| a non-specific defence inducer in preventing clinical signs of infectious bovine rhinotracheitis in calves. | one calf was infected with bovine herpesvirus-1 (bhv-1) and mixed with five other calves, of which one had been vaccinated with a bhv-1 modified live vaccine one month earlier. the other four calves were vaccinated at the time the experimentally infected calf developed the first signs of the disease (fever, depression, nasal discharge), i.e. on post infection day (pid) 2. in addition to the vaccine, two of the four pid 2 vaccinated calves also received a non-specific defence (nsd) inducer (baypa ... | 1996 | 8800541 |
| the cytoplasmic domain of bovine herpesvirus 1 glycoprotein b is important for maintaining conformation and the high-affinity binding site of gb. | bovine herpesvirus 1 (bhv-1) glycoprotein b (gb) has been shown to interact with two types of receptor on madin darby bovine kidney cells. the first receptor is heparan sulfate proteoglycan, whereas the second high-affinity receptor remains unknown. in order to study the structural requirement for gb's high-affinity binding activity, different forms of the gb ectodomain were expressed and compared with authentic gb. by using chemical cross-linking and sucrose gradient centrifugation, we found th ... | 1996 | 8806507 |
| induction of systemic and mucosal immune responses in cotton rats immunized with human adenovirus type 5 recombinants expressing the full and truncated forms of bovine herpesvirus type 1 glycoprotein gd. | we generated both replication-incompetent (had5-gd-e1 and had5-tgd-e1) and replication-competent (had5-gd-e3 and had5-tgd-e3) human adenovirus type 5 (had5) recombinants expressing the full (gd) or truncated form (tgd) of the glycoprotein gd gene of bovine herpevirus type 1 (bhv-1). recombinant gd and tgd expressed by had5-gd-e1 and had5-gd-e3 and by had5-tgd-e1 and had5-tgd-e3, respectively, were recognized by gd-specific monoclonal antibodies (mabs) directed against linear and conformational e ... | 1996 | 8806514 |
| bovine interleukins 2 and 4 expressed in recombinant bovine herpesvirus 1 are biologically active secreted glycoproteins. | the open reading frames encoding bovine interleukin 2 (boil-2) and bovine interleukin 4 (boil-4) were integrated into the unique short segment of the genome of bovine herpesvirus 1 (bhv-1) and expressed under control of the murine cytomegalovirus (mcmv) immediate-early 1 (ie1) enhancer-promoter element or the mcmv early 1 (e1) promoter. madin-darby bovine kidney cells infected with the recombinant viruses secreted boil-2 or boil-4 into the culture medium. secretion was inhibited by the presence ... | 1996 | 8811023 |
| the cytoplasmic domain of bovine herpesvirus 1 glycoprotein b is important for maintaining conformation and the high-affinity binding site of gb | bovine herpesvirus 1 (bhv-1) glycoprotein b (gb) has been shown to interact with two types of receptor on madin darby bovine kidney cells. the first receptor is heparan sulfate proteoglycan, whereas the second high-affinity receptor remains unknown. in order to study the structural requirement for gb's high-affinity binding activity, different forms of the gb ectodomain were expressed and compared with authentic gb. by using chemical cross-linking and sucrose gradient centrifugation, we found th ... | 1996 | 8813035 |
| mucosal and systemic immunity to bovine herpesvirus-1 glycoprotein d confer resistance to viral replication and latency in cattle. | mucosal immunity in the respiratory tract directed against bovine herpesvirus-1 (bhv-1) glycoprotein b forms an effective barrier against bhv-1 replication in cattle. here we investigated the ability of a second bhv-1 glycoprotein, gd, to engender specific antibodies in nasal secretion and serum and protect against infection. we expected gd to give greater protection than gb because anti-gd antibodies prevent viral penetration into cells at much lower concentrations than anti-gb antibodies. calv ... | 1996 | 8821651 |
| efficacy of an experimental bhv-1 subunit giv vaccine in beef calves challenged with bhv-1 in aerosol. | thirty-six beef calves were used to test the efficacy of an experimental truncated bhv-1 glycoprotein (tgiv) vaccine. calves from 1 source and +/- 1 mo of age were randomly divided into 4 groups: 1) control (adjuvant vsa3), 2) vaccinated with tgiv at 3 and 4 mo of age, 3) vaccinated with tgiv at 3 and 7 mo of age, or 4) vaccinated with tgiv at 6 and 7 mo of age. calves were challenged with bhv-1 in aerosol (strain 108) at 7 1/2 mo of age. prior to challenge, serum neutralizing (sn) antibody tite ... | 1996 | 8825995 |
| establishment of a rabbit model for bovine herpesvirus type 5 neurological acute infection. | this study was conducted to evaluate the suitability of the rabbit as a model for bovine, herpesvirus 5 (bhv-5) acute infection. in a preliminary experiment, a total of 24 one-month old new zealand white rabbits were inoculated with bhv-5 or bovine herpesvirus 1 (bhv-1) by the intraconjunctival, intracerebral or intranasal routes. bhv-5 or bhv-1 inoculated in the conjunctiva induced virus proliferation in the eye mucosae and the nasal cavity of rabbits without meningo-encephalitis. on the other ... | 1996 | 8828120 |
| effect of bovine herpesvirus-1 on expression of interleukin-2 receptors and effect of interleukin-12 on lymphocyte proliferation. | expression of the interleukin-2 receptor alpha chain (il-2r alpha) by peripheral blood mononuclear cells (pbmc) from holstein calves, both experimentally-infected with bovine herpesvirus-l (bhv-l) and controls, was measured by flow cytometry. expression of il-2r alpha was 35 percent and 23 percent higher in infected calves than controls, on days 2 and 3 postinfection (pi), respectively. concurrent with this increase in il-2r alpha expression, a significant decrease (p < 0.001) was observed in th ... | 1996 | 8861643 |
| rapid and high sensitivity test for direct detection of bovine herpesvirus-1 genome in clinical samples. | a procedure for direct detection of the bhv-1 genome in clinical samples, including semen, was developed. the method is based on the pcr amplification of a highly conserved dna fragment within the glycoprotein gi sequence of the virus (323 bp between nt 1491 to nt 1814). the method is rapid and highly specific for all 27 subtypes assayed, which are included in the clinical and genetically different groups of bhv-1. the viral origin of the pcr product was assessed by southern hybridization, with ... | 1996 | 8861645 |
| transcriptional and translational expression kinetics of the early gene encoding the bicp27 protein of bovine herpesvirus type 1. | the expression kinetics of an essential trans-regulatory protein, icp27, from herpes simplex virus type 1 correspond to that of an immediate-early gene whose expression increases rapidly upon infection and then decreases as of 7 hr postinfection. in contrast, here we report that the bovine herpesvirus type 1 (bhv-1) homolog bicp27, a 50-kda protein, is expressed as an early gene. both the transcript and protein accumulated gradually reaching peak levels at approximately 12 hr postinfection, afte ... | 1996 | 8862429 |
| a polymerase chain reaction (pcr) assay for the detection of bovine herpesvirus 1 (bhv1) in selectively digested whole bovine semen. | a pcr assay for the detection of bovine herpesvirus type 1 (bhv1) dna in selectively digested whole bovine semen was developed and evaluated. a brief treatment with proteinase-k was used to lyse free virus, virus present in non-sperm cells and virus adhering to the spermatozoa. genomic bovine dna was not released by this treatment. primers and probes were based on the nucleotide sequence of the gd gene. bhv1 virus-spiked split samples were used as positive controls and the pcr products were dete ... | 1996 | 8865582 |
| epidemiologic association between antibody titres against bovine virus diarrhoea virus, rinderpest disease virus and infectious bovine rhinotracheitis virus in a buffalo herd. | the association between antibody titres against bovine virus diarrhoea virus (bvdv), infectious bovine rhinotracheitis virus (ibrv) and rinderpest disease virus (rpdv) in buffaloes was investigated in a cross-sectional study. thirty-six lactating buffaloes from a herd in landhi cattle colony, karachi were bled and serum samples subjected to a micro-neutralisation test at the plum island animal disease center, usa, to categorise each buffalo either as positive or negative against each of 3 viral ... | 1996 | 8888525 |
| synthesis, processing, and oligomerization of bovine herpesvirus 1 ge and gi membrane proteins. | this study reports the identification and initial characterization of the precursors, modified forms, and oligomers of bovine herpesvirus 1 (bhv-1) gi and ge proteins with polyvalent rabbit serum specific for gi or ge. our experiments used the colorado strain of bhv-1 and mutant viruses with insertions of the escherichia coli lacz gene into the predicted ge and gi reading frames. we also translated the ge and gi open reading frames in vitro and expressed them in uninfected cells using eukaryotic ... | 1996 | 8892910 |
| glycoprotein e of bovine herpesvirus type 1 is involved in virus transmission by direct cell-to-cell spread. | in order to identify the role of the bovine herpesvirus type 1 (bhv-1) glycoprotein e (ge) in the viral infection cycle, we have constructed a bhv-1 ge deletion mutant strain (bhv-1 ge-). this strain was assayed in vitro by comparing its growth kinetics with the wild type strain used as a host of the deletion. our results indicate that those conditions which prevent the infection by direct adsorption to the cells (presence of a semi-solid medium or presence of neutralizing antibodies in the medi ... | 1996 | 8896241 |
| protective immunity of bovine herpesvirus-1 (bhv-1) recombinants which express pseudorabies virus (prv) glycoproteins gb, gc, gd and ge. | we have constructed recombinants of bovine herpesvirus-1 (bhv-1) which express pseudorabies virus (prv) gb, gc, gd or ge either individually or in combination. to test the protective immunity, mice were inoculated with these bhv-1 recombinants and challenged three weeks later with virulent prv. a bhv-1 recombinant, bhv-1/tf7-1, which express prv gc, gd, ge and gi but not prv gb, protected all 7 mice from the challenge with 20 ld50 virulent prv and 6 out of 7 from the challenge with 100 ld50 prv, ... | 1996 | 8898277 |
| development of immuno-pcr for diagnosis of bovine herpesvirus 1 infection. | a highly sensitive immuno-pcr method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. this assay attained a high sensitivity of up to 10(7.0) times higher than that of enzyme-linked immunosorbent assay (elisa) or pcr for antigen detection and 10(5.0) times higher than that of elisa for antibody detection. | 1996 | 8904452 |
| construction and characterization of an attenuated bovine herpesvirus type 1 (bhv-1) recombinant virus. | a recombinant bovine herpesvirus type 1 (bhv-1) virus (gal-tk) has been constructed. the gal-tk virus contains a chimeric reporter/marker gene coding for bacterial beta-galactosidase (beta-gal gene) that was inserted stably within the viral tk gene. this resulted in inactivation of the tk gene. the beta-gal gene is under the regulation of a strong, human cytomegalovirus-immediate early (hcmv-ie) promoter and is expressed as an authentic viral-coded gene. even though the one-step growth kinetics ... | 1996 | 8914247 |
| the efficacy of an inactivated ibr vaccine in the prevention of intra-uterine infection and its use in a disease-control programme. | the effects of administration of an established, inactivated ibr vaccine were studied in 30 cows from two herds (one seropositive and one seronegative). all acquired immunity which, after subsequent intratracheal infection with ibr virus, prevented development of symptoms in the cows and protected their foetuses against viral infection in utero. the calves were all healthy and were born at normal term. ten non-vaccinated cows from the seronegative herd responded to the experimental infection wit ... | 1996 | 8919965 |
| production and characterization of bovine herpesvirus 1 glycoprotein b ectodomain derivatives in an hsp70a gene promoter-based expression system. | different derivatives of bovine herpesvirus 1 (bhv-1) glycoprotein b (gb) ectodomain were expressed in a novel heat-shock expression system. the putative ectodomain, gbt, and the n-terminal subunit, gbb, were of the expected molecular weight and were secreted. their production were heat-inducible and the purified proteins were able to elicit antibody responses in mice of a comparable level as induced by authentic gb. the truncated c-terminal subunit, gbct, was retained in the endoplasmic reticul ... | 1996 | 8920833 |
| virulence, immunogenicity and reactivation of seven bovine herpesvirus 1.1 strains: clinical and virological aspects. | specific pathogen-free calves were inoculated intranasally with one of seven strains of bovine herpesvirus 1.1 (bhv 1.1) to identify a highly virulent strain for use in vaccination-challenge experiments. the calves were monitored clinically and virologically. clear differences in virulence between the strains were observed. the iowa strain was the most virulent; the four calves infected with the strain had the most severe clinical signs; two of them died and viraemia was detected in three of the ... | 1996 | 8923716 |
| effect of recombinant bovine interleukin-1 beta in normal calves and in calves infected with bovine herpesvirus type 1. | bovine herpesvirus-1 (bhv-1) is an important pathogen of respiratory infections in cattle. its continuing importance lies in its ability to predispose infected hosts to bacterial infections. in this present study, we determined whether the immunoregulatory effects induced by interleukin-1 (il-1) could stimulate appropriate host defense mechanisms to influence the course of bhv-1 infection in cattle. we first evaluated the effect of different doses (10-1000 ng/kg) of il-1 in normal cattle. a sing ... | 1996 | 8938563 |
| [detection of herpesvirus serum antibodies in red deer (cervus elaphus) imported into the czech republic]. | antibodies reacting in the virus-neutralisation test with bovine herpesvirus (bhv-1), the causative agent of infectious bovine rhinotracheitis (ibr), were demonstrated in a herd of red deer (cervus elaphus) imported into the czech republic. sera from the same collection were later tested against a homologous virus, termed herpesvirus cervidae 1 (hvc-1), isolated from red deer in scotland. out of 165 imported animals, antibodies to bhv-1 were found in 68% and to hvc-1 in 71% of the animals. titre ... | 1996 | 8966967 |
| an attenuated bovine herpesvirus 1 marker vaccine induces a better protection than two inactivated marker vaccines. | a comparative study on the efficacy of 3 bovine herpesvirus 1 (bhv1) marker vaccines was carried out. an attenuated ge-negative vaccine, an inactivated ge-negative vaccine and an experimental gd-subunit vaccine were tested twice in a vaccination-challenge experiment in cattle. the attenuated vaccine induced the best clinical protection as evidenced by the total absence of clinical signs and fever in cattle. in addition, the attenuated vaccine reduced the shedding of challenge virus significantly ... | 1996 | 8972048 |
| prediction of potential cytotoxic t lymphocyte epitopes of bovine herpesvirus 1 based on allele-specific peptide motifs and proteolytic cleavage specificities. | major histocompatibility complex (mhc) class i molecules present endogenous peptides to cytotoxic t lymphocytes (ctls). elucidation of ctl epitopes of intracellular pathogens helps in designing better vaccines to control economically important human and animal diseases. in this study, candidate epitopes that are potentially available for presentation to the ctls via five bovine mhc class i molecules have been identified. this was accomplished by using the computer programs "find-patterns" and "p ... | 1996 | 8972566 |
| identification and characterization of the bovine herpesvirus 1 ul7 gene and gene product which are not essential for virus replication in cell culture. | the ul7 gene of bovine herpesvirus 1 (bhv-1) strain schönböken was found at a position and in a context predicted from the gene order in the prototype alphaherpesvirus herpes simplex virus type 1. the gene and flanking regions were sequenced, the ul7 rna and protein were characterized, and 98.3% of the ul7 open reading frame was deleted from the viral genome without destroying productive virus replication. concomitant deletion of nine 3' codons from the bhv-1 ul6 orf and 77 amino acids from the ... | 1996 | 8551568 |
| identification and characterization of a bovine herpesvirus-1 (bhv-1) glycoprotein gl which is required for proper antigenicity, processing, and transport of bhv-1 glycoprotein gh. | dna sequence analysis of the bovine herpesvirus-1 (bhv-1) genome revealed the presence of an open reading frame named ul1 which exhibited limited homology to glycoprotein gl of herpes simplex virus-1 (s. k. khattar, s. van drunen littel-van den hurk, l. a. babiuk, and s. k. tikoo, virology 213, 28-37). to identify the bhv-1 ul1 protein, rabbit antisera were prepared against two synthetic peptides that were predicted by computer analysis to encompass antigenic epitopes. sera against both peptides ... | 1996 | 8623555 |
| identification and characterization of bicp27, an early protein of bovine herpesvirus 1 which may stimulate mrna 3' processing. | sequence analysis of the left genomic terminus of bovine herpesvirus 1 (bhv-1) revealed two convergently transcribed genes with 3' ends about 300 bp apart. the gene on the left is the previously described circ gene; that on the right was found to encode a protein of 400 amino acids which was designated bicp27 because of its homology to icp27 (vmw63) of herpes simplex virus 1 (hsv-1) and related proteins from other alpha- beta- and gammaherpesviruses. rabbit antisera raised against a synthetic ol ... | 1996 | 8627249 |
| bovine herpesvirus 1 ul49.5 homolog gene encodes a novel viral envelope protein that forms a disulfide-linked complex with a second virion structural protein. | we previously reported that the genome of bovine herpesvirus 1 (bhv-1) contains an open reading frame (orf) homologous to the herpes simplex virus ul49.5 orf, and as with the herpes simplex virus ul49.5 orf, the deduced amino acid sequence of the bhv-1 ul49.5 homolog (ul49.5h) contains features characteristic of an integral membrane protein, implying that it may constitute a functional gene encoding a novel viral envelope protein. this communication reports on the identification of the bhv-1 ul4 ... | 1996 | 8627662 |
| glycoprotein bb, the n-terminal subunit of bovine herpesvirus 1 gb, can bind to heparan sulfate on the surfaces of madin-darby bovine kidney cells. | the present study confirms our previous findings made by using heparin affinity chromatography that bovine herpesvirus 1 gb can bind to heparin-like structures. in order to locate the functional domain for heparin binding, we expressed the extracellular portion of gb (gbt) and the large subunit of gb (gbb) in madin darby bovine kidney (mdbk) cells under the control of the bovine heat shock protein 70a gene promoter. the recombinant gbt and gbb were both efficiently secreted from the transfected ... | 1996 | 8627732 |
| bovine herpesvirus 1 u(s) open reading frame 4 encodes a glycoproteoglycan. | sequence analysis of the short unique (us) segment of the bovine herpesvirus 1 (bhv-1) genome predicted that the us open reading frame (orf) 4 encodes a protein with homology to glycoprotein g (gg) of other alpha-herpesviruses (p. leung-tack, j.-c. audonnet, and m. riviere, virology 199:409-421, 1994). rna analysis showed that the us orf4 is contained within two transcripts of 3.5 and 1.8 kb. the 3.5 kb rna represents a structurally bicistronic rna which encompasses the us orf3 and us orf4, wher ... | 1996 | 8627780 |
| construction of bovine herpesvirus-1 (bhv-1) recombinants which express pseudorabies virus (prv) glycoproteins gb, gc, gd, and ge. | we have improved the method for constructing recombinants of bovine herpesvirus type-1 (bhv-1). using this method, we constructed three recombinants in which the pseudorabies virus (prv) thymidine kinase (tk) gene was inserted at three different sites in the unique short region of bhv-1. these three sites are located in the open reading frame of ge, gg and gi genes. previously, two sites (tk and gc) had been used to insert foreign dna fragments to bhv-1 genome. therefore we now have 5 sites in b ... | 1996 | 8629951 |
| pathology of bovine abortion and newborn calf death caused by dual infection with chlamydia psittaci and infectious bovine rhinotracheitis virus. | nine aborted fetuses and one newborn calf, diagnosed as chlamydia psittaci (c. psittaci) infection, were pathologically examined. the characteristic lesions in the liver were focal necrosis in 9 aborted fetuses and granulomatous necrotic foci in the newborn calf. moderate numbers of intranuclear inclusion bodies were found in necrotic foci of the liver, spleen, thymus, lymph nodes, adrenal gland, kidney, lung and forestomach. immunohistologically, a small number of c. psittaci antigens was demon ... | 1996 | 8645759 |
| identification and characterization of the pseudorabies virus ul3.5 protein, which is involved in virus egress. | alphaherpesvirus genomes exhibit a generally collinear gene arrangement, and most of their genes are conserved among the different members of the subfamily. among the exceptions is the ul3.5 gene of pseudorabies virus (prv) for which positional homologs have been detected in the genomes of varicella-zoster virus, equine herpesvirus 1, and bovine herpesvirus 1 but not in the genomes of herpes simplex virus types 1 and 2. to identify and characterize the predicted 224 amino acid ul3.5 protein of p ... | 1996 | 8648685 |
| the latency-related gene of bovine herpesvirus 1 encodes a product which inhibits cell cycle progression. | bovine herpesvirus 1 (bhv-1) establishes a latent infection in the sensory ganglionic neurons of cattle. the exclusive viral rna expressed in a latent infection is the latency-related (lr) rna, suggesting that it regulates some aspect of a latent infection. during the course of a productive infection, alphaherpesviruses induce certain events which occur during cell cycle progression. consequently, we hypothesized that a bhv-1 infection might induce events in neurons which occur during cell cycle ... | 1996 | 8648716 |
| inactivated bovine herpesvirus 1 induces apoptotic cell death of mitogen-stimulated bovine peripheral blood mononuclear cells. | bovine herpesvirus 1 (bhv-1) is able to inhibit the proliferation of bovine peripheral blood mononuclear cells. here, we have demonstrated that live bhv-1 and, interestingly, inactivated bhv-1 can induce apoptosis of mitogen-stimulated bovine peripheral blood mononuclear cells in vitro. | 1996 | 8648752 |
| differential control of transcription by homologous homeodomain coregulators. | the human herpes simplex virus type 1 (hsv-1) transactivator vp16 and its homolog from bovine herpes-virus 1 (bhv-1) can each recruit the human homeodomain protein oct-1 into a transcriptional regulatory complex. here, we show that these two oct-1 coregulators possess similar, if not identical, homeodomain recognition properties but possess different virus-specific cis-regulatory specificities: the hsv-1 vp-16 protein activates transcription from the hsv-1 vp16 response element, and the bhv-1 vp ... | 1996 | 8649408 |
| sequence and expression of a bovine herpesvirus-1 gene homologous to the glycoprotein k-encoding gene of herpes simplex virus-1. | in the bovine herpes virus-1 (bhv-1) genome, a gene equivalent to the glycoprotein k (gk)-encoding gene of other herpesviruses was identified and sequenced. the primary translation product is predicted to comprise 338 amino acids (aa) and to exhibit a molecular mass of 37.5 kda. it possesses characteristics typical for membrane glycoproteins including a potential cleavable signal sequence, three transmembrane domains and two potential n-linked glycosylation sites. comparison to the gk proteins o ... | 1996 | 8654942 |
| expression of bovine interleukin-1 beta in a bovine herpesvirus-1 vector: in vitro analysis. | in order to evaluate whether bovine herpesvirus-1 (bhv-1) could be used as a live viral vector for the expression of cytokines, we constructed a recombinant bhv-1 expressing bovine interleukin-1 beta (boil-1 beta). the boil-1 beta coding sequence, corresponding to the cleaved mature product, was fused with the bhv-1 glycoprotein c (gc) signal peptide sequence; the resultant gc-boil-1 beta fusion gene was recombined into the gc locus of the bhv-1 genome. southern blot analysis confirmed the prope ... | 1996 | 8661416 |
| [interactions between bronchial epithelial cells and extracellular matrix proteins]. | attachment and migration of bronchial epithelial cells are important in re-epithelialization after tissue injury. we hypothesized that inflammatory cytokines alter bronchial epithelial cell attachment and migration. to test this hypothesis, we studied the effects of mononuclear-cell-conditioned medium on attachment and migration of bronchial epithelial cells in response to fibronectin in vitro. this medium was prepared from bovine blood mononuclear cells that were stimulated with concanavalin a; ... | 1996 | 9216202 |
| prevalence of antibodies to bluetongue, bovine herpesvirus 1 and bovine viral diarrhea/mucosal disease viruses in water buffaloes in minas gerais state, brazil. | a serological survey to detect water buffaloes with antibodies to bluetongue virus (btv), bovine herpesvirus 1 (bhv 1) and bovine viral diarrhea/mucosal disease virus (bvd/mdv) was performed in minas gerais state, brazil. precipitating antibodies against btv were detected by the agar gel immunodiffusion test (agid) in 54.4% of the serum samples tested. microplate serum-neutralization tests revealed that 14.7% and 52.7% of the water buffaloes had antibodies to bhv 1 and bvd/mdv, respectively. the ... | 1996 | 9091990 |
| restriction endonuclease analysis of dna from indian isolates of bovine herpesvirus 1. | the genomic variation of three isolates of bovine herpes virus 1 (bhv-1) originating from different geographical regions and displaying different clinical symptoms were studied by restriction analysis using four different restriction endonucleases. ecori displayed an uniform restriction pattern for all the isolates and hindiii showed marginal variation among the isolates. bsteii and psti displayed unique restriction patterns, based on which the isolates could be grouped into two subgroups of bhv ... | 1996 | 9171462 |
| functional expression of the bovine herpesvirus 1 alkaline deoxyribonuclease (ul12) in escherichia coli. | sequence analysis within the unique long segment of the bovine herpesvirus 1 (bhv-1; infectious bovine rhinotracheitis virus) genome identified an open reading frame whose deduced protein product of 487 amino acids exhibited homology to alkaline deoxyribonucleases (dnases) of other herpesviruses. to determine this bhv-1 gene product has nuclease activity, the gene designated ul12 was inserted into the vector pet-28a(+) and expressed in escherichia coli as an oligohistidine-tagged protein. upon i ... | 1996 | 9526549 |
| direct detection of bovine herpes virus-1 dna from cell culture fluids using polymerase chain reaction. | a pair of oligomers of 20 and 23 bp were designed for amplifying a 381 bp sequence from glycoprotein iv gene of bovine herpesvirus 1. the primer pairs were used for amplifying genomic dna of bhv-1 directly from cell culture fluids under different experimental conditions such as, untreated cell culture fluid, thermal denaturation and proteinase k treatment in presence of detergent. the results reveal that direct thermal denaturation of cell culture fluid is sufficient to detect the virus by polym ... | 1996 | 9055637 |
| unidirectional complementation between glycoprotein b homologues of pseudorabies virus and bovine herpesvirus 1 is determined by the carboxy-terminal part of the molecule. | the most highly conserved glycoproteins in herpesviruses, homologues of glycoprotein b (gb) of herpes simplex virus, have been shown to play essential roles in membrane fusion during penetration and direct cell-to-cell spread of herpes virions. in studies aimed at assessing whether sequence conservation is reflected in the conservation of functional properties, we previously showed that bovine herpesvirus 1 (bhv-1) gb was able to functionally complement a gb- prv mutant. to analyse in detail the ... | 1995 | 9049369 |
| [detection of bovine infectious rhinotracheitis virus by hybridization using nonradioactive dna-probes]. | biotin-labeled dna probes for infectious bovine rhinotracheitis virus also known as bovine herpesvirus-1 (bhv-1) have been developed. the procedure is based on dot-blot hybridization using biotin-labeled bacteriophage m13 and plasmid probes containing cloned psti and ecori-psti restriction fragments of viral genome. the probes obtained were used to detect viral nucleic acids in specimens of bovine spermatic fluid or nasal swabs of calves. the method is simple and rapid, taking less than 24 h, an ... | 1995 | 8686268 |
| characterization of the camel skin cell line dubca. | a skin fibroblast cell culture was established from a 2-month-old dromedary foetus. the cells were transformed by infection with sv40 and cloned in soft agar. the established cell line is now designated dubca cells (dubai camel) and has been in permanent culture for 95 passages. the cell culture was examined morphologically, chromosome preparations made and dna fingerprinting performed by hybridization with the oligonucleotide probe (gtg)5. sv40 large t antigen was detected by western blotting. ... | 1995 | 8556315 |
| persistence of bovine herpesvirus-1-specific antibodies in cattle after intranasal vaccination with a live virus vaccine. | to study the development and persistence of circulating antibodies directed against bovine herpesvirus-1 (bhv-1) induced by vaccination, approximately 80 per cent of the seronegative cows in four partly seronegative dairy herds were vaccinated once with a temperature-sensitive live virus vaccine. most (83 per cent) of the vaccinated animals developed antibodies to bhv-1 within two months after the vaccination. in the same period, 21 per cent of the unvaccinated control cattle also seroconverted, ... | 1995 | 8560685 |
| detection of bovine herpesvirus 1 in the semen of experimentally infected bulls by dot-blot hybridisation, polymerase chain reaction and virus isolation. | two 18-month-old bovine herpesvirus 1 (bhv1)-seronegative bulls were inoculated experimentally with bhv1 via their prepuces. semen collected at intervals was examined by optimised virus isolation, dot-blot hybridisation and the polymerase chain reaction (pcr) for detection of bhv1, and the infection was monitored serologically by using a virus neutralisation test. antibodies were first detected 10 days after inoculation and were still present 40 days after inoculation. semen collected from four ... | 1995 | 8525113 |
| differences in the role of glycoprotein c of hsv-1 and hsv-2 in viral binding may contribute to serotype differences in cell tropism. | heparan sulfate serves as a receptor for several herpesviruses. for herpes simplex virus 1 (hsv-1), pseudorabies virus, and bovine herpesvirus 1, glycoprotein c homologues have been shown to mediate the binding to cell-surface heparan sulfate. it has been assumed that glycoprotein c of hsv-2 (gc-2) plays a similar role in hsv-2 entry, but this has not been established experimentally. we first determined, using heparin-affinity chromatography, that gc-2 is a heparin-binding glycoprotein. to exami ... | 1995 | 8525631 |
| virulence and genotype of a bovine herpesvirus 1 isolate from semen of a subclinically infected bull. | a bovine herpesvirus 1 (bhv-1) isolate from the semen of a subclinically infected bull was administered to cattle by various routes to assess its virulence. cattle that were artificially inseminated or inoculated intrapreputially did not develop clinical signs, but did transmit the virus to contact cattle. however, the isolate induced severe signs of rhinotracheitis and vulvovaginitis in cattle that were inoculated by the intravaginal, intranasal or intravenous routes, but did not infect the fet ... | 1995 | 8533214 |
| isotype-specific antibody responses to bovine herpesvirus 1 in sera and mucosal secretions of calves after experimental reinfection and after reactivation. | isotype-specific antibody responses to bovine herpesvirus 1 (bhv1) were measured in sera, nasal, ocular and genital secretions of calves that were reinfected with bhv1 and 6 weeks later treated with corticosteroids to reactivate putative latent virus. after reinfection and after reactivation, no bhv1-specific igm antibody response was detected. the serum iga response was only transiently detectable after reinfection and again appeared rapidly after reactivation in most calves. most calves showed ... | 1995 | 8533302 |
| lymphocyte proliferative responses to recombinant bovine herpes virus type 1 (bhv-1) glycoprotein gd (giv) in immune cattle: identification of a t cell epitope. | the lymphocyte proliferative response to bhv-1 in immune cattle was compared to recombinant wild-type gd and truncated gd produced from recombinant vaccinia viruses. the response exhibited by recombinant proteins was comparable to the response induced by bhv-1 suggesting that gd is the major target structure for stimulation of bovine lymphocytes. analysis of the proliferative response using vaccinia virus vectors expressing various modified forms of gd identified a region between residues 165 an ... | 1995 | 8546801 |
| bovine herpesvirus-1 infection reduces bronchial epithelial cell migration to extracellular matrix proteins. | repair of airway epithelium after viral infection involves migration of epithelial cells to cover injured, denuded areas. we determined whether viral infection reduces the capability of bronchial epithelial cells to migrate and to attach to extracellular matrix proteins. inoculation of bovine bronchial epithelial cells in vitro with bovine herpesvirus-1 reduced their ability to migrate in two different assays of cell migration. when attachment assays were performed, fewer cells attached to both ... | 1995 | 7864142 |
| delineation of the essential function of bovine herpesvirus 1 gd: an indication for the modulatory role of gd in virus entry. | the entry process of alphaherpesviruses consists of two steps, initial virus attachment and subsequent virus penetration involving membrane fusion. glycoprotein d (gd) of the alphaherpesvirus bovine herpesvirus 1 (bhv 1) is an essential envelope protein, and it has been previously documented that gd plays a significant part in both of the virus entry steps. in order to gain further insight into the virus entry process, we attempted to define the essential function of bhv 1 gd. we replaced the gd ... | 1995 | 7886947 |
| amino acid substitutions in glycoprotein d mediate the ability of fusion from without-positive herpes simplex virus type 1 strains to penetrate at 4 degrees and in the presence of soluble glycoprotein d. | in former studies, we described herpes simplex virus type 1 (hsv-1) strains ang, ang path and hszp as strains with special fusion activities, caused by mutations in the syn 3 locus. in this report, we describe the special penetration properties of these strains: their ability to penetrate at 4 degrees and to overcome the infection block caused by soluble glycoprotein d-1 (gd-1) in the medium. using intertypic recombinants of hsv-1 strains ang path and kos, we showed that these penetration proper ... | 1995 | 8724859 |
| detection of bovine herpesvirus 1 in clinical samples by the polymerase chain reaction. | a pcr assay with primers selected from the gl gene and flanking a 468 bp dna fragment was tested on clinical samples. of 27 samples (nasal swabs, lung, lymph nodes, tracheal mucosa) collected from 16 different outbreaks in scotland, 18 were positive by pcr and 13 by virus isolation. some samples of isolated dna had to be diluted by a factor 50-100 to obtain a positive pcr result. pcr assay could detect the bhv-1 positive samples collected from different european regions, namely from slovakia, it ... | 1995 | 8582261 |
| [clinical experiences in the use of para-immunity inducers in cattle during animal exhibitions]. | ibr positive cattle after vaccination with dead vaccine may excrete ibr/ipv (bhv-1) virus when facing a stress situation. on cattle exhibitions the share of such individuals may vary between 8 and 34%. since 1988 in order to protect animals against infections ibr negative cattle and cows on 7 regional, one national and one international exhibitions were treated with duphamun and baypamun, respectively. after returning of animals to their owner ibr outbreaks were confirmed in 8 forms. this is low ... | 1995 | 8593769 |
| monitoring experimental alcelaphine herpesvirus-1 infection in cattle by nucleic-acid hybridization and pcr. | the dna probe sw15 derived from the laboratory-attenuated alcelaphine herpesvirus-1 (ahv-1) strain wc11 as well as from the polymerase chain-reaction test (hsu, shih, castro & zee 1990), was used to detect viral dna of malignant catarrhal fever (mcf) in six experimentally infected cattle. heparinized blood samples were collected and tested at least three times a week over a period of up to 142 d. results of hybridization and pcr tests were compared with the results of clinical examinations, and ... | 1995 | 8600434 |
| bovine herpesvirus meningoencephalitis association with infectious bovine rhinotracheitis (ibr) vaccine. | during 1992, on a farm in the tokachi district of hokkaido, japan, approximately 20 holstein-friesian calves showed neuroparalysis and died within 7-10 days after routine vaccination. six male calves, aged about 1.5 months, were submitted to our laboratory for pathological examination and diagnosed as acute or subacute necrotizing meningoencephalitis due to bovine herpes virus (bhv) infection. the main necropsy findings included a few hemorrhages or clots, and malacic lesions localized in the co ... | 1995 | 8615076 |
| fine mapping of bovine herpesvirus-1 (bhv-1) glycoprotein d (gd) neutralizing epitopes by type-specific monoclonal antibodies and sequence comparison with bhv-5 gd. | overlapping fragments of the bovine herpesvirus-1 (bhv-1) glycoprotein (gd) orf were expressed as trpe-gd fusion proteins in escherichia coli to map linear neutralizing epitopes defined by bhv-1-specific mabs. the mabs 3402 and r54 reacted with the expressed fragments on western blots that located the epitopes between the amino acids 52-126 and 165-216, respectively, of gd. bovine covalescent sera with high neutralizing antibody titers against bhv-1 reacted with these bacterially expressed prote ... | 1995 | 7530392 |
| restriction endonuclease analysis of bovine herpesvirus type 1 isolates from calves with fatal encephalitis: comparison with vaccine virus. | meningo-encephalitis in feedlot cattle sporadically occurred in the tokachi area in northern japan. the calves had been vaccinated intranasally with a mixed live-vaccine (infectious bovine rhinotracheitis virus, bovine viral diarrhea-mucosal disease virus, and parainfluenza 3 virus) for which intramuscular inoculation was indicated. two additional live vaccines, bovine adenovirus type 7 and bovine respiratory syncytical virus, had been inoculated simultaneously. eleven isolates of bovine herpesv ... | 1995 | 7548427 |
| a glycoprotein e deletion mutant of bovine herpesvirus 1 infects the same limited number of tissues in calves as wild-type virus, but for a shorter period. | to gain insight into the role of glycoprotein e of bovine herpesvirus 1 (bhv-1), we compared the distribution of wild-type (wt) bhv-1 with that of a ge deletion mutant (ge-) in calves after intranasal inoculation. the wt-infected calves had severe clinical signs, but the ge(-)-infected calves were virtually free of clinical signs. at 3, 4, 7, 8, 44, 45, 50 and 51 days post-infection (p.i.), one calf from each group was killed and tissues were collected for virus isolation and pcr analysis. at 3, ... | 1995 | 7561782 |
| sequence analysis of the ul39, ul38, and ul37 homologues of bovine herpesvirus 1 and expression studies of ul40 and ul39, the subunits of ribonucleotide reductase. | we sequenced the region of the bovine herpesvirus type 1 (bhv-1) genome corresponding to map units 0.172-0.230 (7964 bp), representing the ul39, ul38, and ul37 homologues of herpes simplex virus which encode the large subunit of ribonucleotide reductase (rr) and components of the viral capsid and the tegument, respectively. to discriminate between two potential initiator augs of the ul39 gene, the 5' end of the mrna was mapped by s1 nuclease protection assays. comparison of the amino acid sequen ... | 1995 | 7571445 |
| abortion in heifers inoculated with a thymidine kinase-negative recombinant of bovine herpesvirus 1. | the copper isolate of bovine herpesvirus 1 (bhv-1) was used to produce a thymidine kinase-negative (tk-) recombinant by insertion of a beta-galactosidase (bgal) expression cassette into the tk coding region. the recombinant virus (tk- bgal+) was tested for abortifacient activity in cattle by inoculation of 5 pregnant heifers at 25 to 29 weeks gestation. five additional heifers were inoculated with the cooper tk-positive (tk+) virus to serve as controls. after inoculation, both groups of heifers ... | 1995 | 7574153 |
| cloning of 2.4 kb bovine herpesvirus-1 dna fragment containing glycoprotein iii gene into puc18 plasmid vector. | a recombinant pbr322 plasmid containing bovine herpesvirus-1 hindiii 'i' fragment was analysed using ecori and bamhi restriction endonucleases. this recombinant plasmid was labelled with [alpha 32p]datp and hybridized with southern blot of hindiii digested bhv-1 dna fragments. a 2.4 kb double digested ecori-bamhi fragment of hindiii 'i' was subcloned into puc18 plasmid to get complete giii gene. the recombinant puc18 plasmid was analysed for 2.4 kb bhv-1 dna insert by restriction digestion with ... | 1995 | 7590952 |
| study of neutralizing monoclonal antibodies to bovine herpes virus type-1 (cooper strain) by immunoperoxidase and immunoelectron microscopy. | three neutralizing monoclonal antibodies (mabs) that are specific against bovine herpes virus type-1 (bhv-1) were studied as to their viral specificity by immunoperoxidase and immunoelectron microscopy. microscopic examination of gbk bhv-1 infected cells revealed peroxidase activity represented by red-brown granular deposits in the nucleus and cytoplasm. no immunoperoxidase activity was observed in negative controls. for the ultrastructural observations, two approaches were used. firstly we test ... | 1995 | 7599599 |