Publications

haemagogus leucocelaenus and haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in brazil, 2016-2018.	the yellow fever virus (yfv) caused a severe outbreak in brazil in 2016-2018 that rapidly spread across the atlantic forest in its most populated region without viral circulation for almost 80 years. a comprehensive entomological survey combining analysis of distribution, abundance and yfv natural infection in mosquitoes captured before and during the outbreak was conducted in 44 municipalities of five brazilian states. in total, 17,662 mosquitoes of 89 species were collected. before evidence of ...	2019	30866775
entomo-virological surveillance strategy for dengue, zika and chikungunya arboviruses in field-caught aedes mosquitoes in an endemic urban area of the northeast of brazil.	aedes spp. are considered the main vectors of dengue (denv), zika (zikv) and chikungunya (chikv) viruses in the world. arbovirus detection in aedes mosquitoes can alert authorities to possible outbreaks, reducing the impact of these diseases. the purpose of this study was to perform an operational strategy for virological surveillance of denv, zikv and chikv in adult aedes aegypti and aedes albopictus mosquitoes captured at different key-sites in an endemic urban area of the northeast region of ...	2019	31194961
zika virus detection, isolation and genome sequencing through culicidae sampling during the epidemic in vitória, espírito santo, brazil.	zika virus (zikv) has been isolated from many mosquito species in nature, but it is believed that the main vectors in urban environments are species of the genus aedes. here, we detected and isolated zikv in samples from aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus, collected during the zika epidemic in vitória, southeast brazil. using quantitative real-time polymerase chain reaction, zikv detection was performed in mosquito samples collected from february to april 2016.	2019	31068218
dataset for mosquito collections on big pine key, florida, usa.	the florida keys mosquito control district has deployed dry ice-baited light traps to monitor mosquito (diptera: culicidae) populations throughout the florida keys starting in 1998. american biophysics company traps were deployed throughout the year at the same collection point. traps were placed in the late afternoon and collected the following morning. common mosquitoes are the black salt marsh mosquito, aedes taeniorhynchus (wiedemann), the crabhole mosquito, deinocerites cancer theobald, the ...	2019	31667279
assessing the blood meal hosts of culex quinquefasciatus and aedes taeniorhynchus in isla santa cruz, galápagos.	blood meal host selection by mosquito vectors is an important component in understanding disease dynamics of pathogens that threaten endemic fauna in isolated islands such as galápagos. research on the feeding behavior of mosquitoes can provide clues to the hosts and vectors involved in disease transmission. this information is particularly critical for endemic wildlife fauna in island systems that have evolved without resistance to novel diseases such as avian malaria. the aims of this study we ...	2019	31842984
mosquito abundance in a dirofilaria immitis hotspot in the eastern state of rio de janeiro, brazil.	coastal lowlands in the state of rio de janeiro, brazil, support high numbers of heartworm-infected dogs. microfilariae of heartworm need to be ingested by a potencial mosquito vector in order to develop into infective larvae and infect a new host. ochlerotatus taeniorhynchus and ochlerotatus scapularis are the primary vector species in the coastal lowlands of metropolitan rio de janeiro; thus, the aim of this study was to investigate whether these two species were abundant enough at the heartwo ...	2019	31796177
the impact of hurricane irma on population density of the black salt-marsh mosquito, aedes taeniorhynchus, in collier county, florida.	in 2017, the southeastern usa and caribbean endured an extremely active year for tropical storm and hurricane activity. on september 10, 2017, hurricane irma made a 2nd landfall in florida, striking collier county-including naples and marco island, fl. areas affected by hurricane irma are abundant in salt-marsh and mangrove swamps, prime habitats for the black salt-marsh mosquito, aedes taeniorhynchus. using both human landing rate and biogents bg-counter trap data, here we report the year-to-ye ...	2019	31442180
microsatellite markers transferability in culex nigripalpus (diptera: culicidae).	microsatellite loci specifically designed and optimized for secondary vectors and neglected species of mosquitoes are lacking. cross-species transferability of microsatellite loci between close taxa has been used as a reliable option, being successfully done for aedes taeniorhynchus wiedemann (diptera: culicidae), triatoma sordida stål (hemiptera: reduviidae), and aedes fluviatilis lutz (diptera: culicidae). nonetheless, prior to the utilization of transferred microsatellites, they must be asses ...	2018	29767738
detection of novel and recognized rna viruses in mosquitoes from the yucatan peninsula of mexico using metagenomics and characterization of their in vitro host ranges.	a metagenomics approach was used to detect novel and recognized rna viruses in mosquitoes from the yucatan peninsula of mexico. a total of 1359 mosquitoes of 7 species and 5 genera (aedes, anopheles, culex, mansonia and psorophora) were sorted into 37 pools, homogenized and inoculated onto monolayers of aedes albopictus (c6/36) cells. a second blind passage was performed and then total rna was extracted and analysed by rna-seq. two novel viruses, designated uxmal virus and mayapan virus, were id ...	2018	30412047
screening of natural wolbachia infection in aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus from guadeloupe (french west indies).	guadeloupe islands are threatened by several mosquito-borne viruses such as dengue, chikungunya, zika and west nile virus. it appears essential to look for alternative mosquito control methods such as the incompatible insect technique (itt) aiming at sterilizing wild females by inundative releases of incompatible males. before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium wolbachia regarding the local mosquito populatio ...	2018	29908171
effects of mosquito control adulticides on sterile cochliomyia hominivorax (diptera: calliphoridae).	effects of mosquito control adulticides on sterile screwworm flies, cochliomyia hominivorax (coquerel) (diptera: calliphoridae), were investigated via bottle bioassays, outdoor cage tests, and exposure to treated vegetation. in bottle bioassays, 43 μg of permethrin via dilution of evoluer, 474.56 μg of malathion via dilution of fyfanon, and 25 μg of naled via dilution of dibrom concentrate were used to challenge screwworm flies. permethrin was more toxic to screwworm flies than was malathion, wh ...	2018	29365134
zika virus vector competency of mosquitoes, gulf coast, united states.	zika virus has recently spread throughout the americas. although aedes aegypti mosquitoes are considered the primary vector, culex quinquefasciatus and mosquitoes of other species may also be vectors. we tested cx. quinquefasciatus and ae. taeniorhynchus mosquitoes from the us gulf coast; both were refractory to infection and incapable of transmission.	2017	28005002
the distribution of mosquitoes across an altitudinal gradient in the galapagos islands.	an avian malaria parasite (genus plasmodium) has been detected consistently in the galapagos penguin (spheniscus mendiculus) and less frequently in some passerines. we sampled three resident mosquito species (aedes taeniorhynchus, culex quinquefasciatus, and aedes aegypti) using cdc light and gravid traps on three islands in 2012, 2013, and 2014. we sampled along altitudinal gradients to ask whether there are mosquito-free refugia at higher elevations as there are in hawaii. we captured both ae. ...	2017	29125252
non-human primate antibody response to mosquito salivary proteins: implications for dengue virus transmission in puerto rico.	an important step to incriminate a mosquito as a vector of a disease pathogen is finding evidence of direct contact between the mosquito and humans. typically, this is accomplished through landing/biting catches, or host blood meal analysis in engorged mosquitoes via immunologic assays. an alternate approach is to identify the presence of specific mosquito anti-saliva protein antibodies in the blood of exposed hosts. following the discovery of dengue infected, free roaming non-human primates in ...	2016	27593498
the epidemiology of rift valley fever in mayotte: insights and perspectives from 11 years of data.	rift valley fever (rvf) is a zoonotic arboviral disease that is a threat to human health, animal health and production, mainly in sub-saharan africa. rvf virus dynamics have been poorly studied due to data scarcity. on the island of mayotte in the indian ocean, off the southeastern african coast, rvf has been present since at least 2004. several retrospective and prospective serological surveys in livestock have been conducted over eleven years (2004-15). these data are collated and presented he ...	2016	27331402
countrywide serological evaluation of canine prevalence for anaplasma spp., borrelia burgdorferi (sensu lato), dirofilaria immitis and ehrlichia canis in mexico.	canine vector-borne diseases (cvbd) have become a major concern for canine and human public health. the aim of the study described here is to add epidemiological data regarding four pathogens responsible for cvbd, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducted in mexico.	2016	27474020
molecular detection of flaviviruses and alphaviruses in mosquitoes (diptera: culicidae) from coastal ecosystems in the colombian caribbean.	arboviruses belonging to the genera flavivirus and alphavirus were detected in mosquitoes in a rural area of san bernardo del viento (córdoba, colombia). a total of 22,180 mosquitoes were collected, sorted into 2,102 pools, and tested by generic/nested reverse transcription-polymerase chain reaction. venezuelan equine encephalitis virus, dengue virus, west nile virus, st. louis encephalitis virus, yellow fever virus, and culex flavivirus were detected and identified by sequencing. the detection ...	2016	27706377
wolbachia biocontrol strategies for arboviral diseases and the potential influence of resident wolbachia strains in mosquitoes.	arboviruses transmitted by mosquitoes are a major cause of human disease worldwide. the absence of vaccines and effective vector control strategies has resulted in the need for novel mosquito control strategies. the endosymbiotic bacterium wolbachia has been proposed to form the basis for an effective mosquito biocontrol strategy. resident strains of wolbachia inhibit viral replication in drosophila fruit flies and induce a reproductive phenotype known as cytoplasmic incompatibility that allows ...	2016	26925368
vertical transmission of zika virus in aedes aegypti mosquitoes.	previous experimental studies have demonstrated that a number of mosquito-borne flavivirus pathogens are vertically transmitted in their insect vectors, providing a mechanism for these arboviruses to persist during adverse climatic conditions or in the absence of a susceptible vertebrate host. in this study, designed to test whether zika virus (zikv) could be vertically transmitted, female aedes aegypti and aedes albopictus were injected with zikv, and their f1 adult progeny were tested for zikv ...	2016	27573623
research contributing to improvements in controlling florida's mosquitoes and mosquito-borne diseases.	research on mosquitoes and mosquito-borne diseases has contributed to improvements in providing effective, efficient, and environmentally proper mosquito control. florida has benefitted from several research accomplishments that have increased the state's mosquito control capabilities. research with florida's mosquitoes has resulted in the development of ecologically sound management of mosquito impoundments on florida's east coast. this strategy, called rotational impoundment management (rim), ...	2016	27690112
has rift valley fever virus evolved with increasing severity in human populations in east africa?	rift valley fever (rvf) outbreaks have occurred across eastern africa from 1912 to 2010 approximately every 4-15 years, most of which have not been accompanied by significant epidemics in human populations. however, human epidemics during rvf outbreaks in eastern africa have involved 478 deaths in 1998, 1107 reported cases with 350 deaths from 2006 to 2007 and 1174 cases with 241 deaths in 2008. we review the history of rvf outbreaks in eastern africa to identify the epidemiological factors that ...	2016	27329846
mosquito oviposition behavior and vector control.	the burden of gene transfer from one mosquito generation to the next falls on the female and her eggs. the selection of an oviposition site that guarantees egg and larval survival is a critical step in the reproductive process. the dangers associated with ephemeral aquatic habitats, lengthy droughts, freezing winters, and the absence of larval nutrition makes careful oviposition site selection by a female mosquito extremely important. mosquito species exhibit a remarkable diversity of ovipositio ...	2016	27869724
in-depth study of mollivirus sibericum, a new 30,000-y-old giant virus infecting acanthamoeba.	acanthamoeba species are infected by the largest known dna viruses. these include icosahedral mimiviruses, amphora-shaped pandoraviruses, and pithovirus sibericum, the latter one isolated from 30,000-y-old permafrost. mollivirus sibericum, a fourth type of giant virus, was isolated from the same permafrost sample. its approximately spherical virion (0.6-µm diameter) encloses a 651-kb gc-rich genome encoding 523 proteins of which 64% are orfans; 16% have their closest homolog in pandoraviruses an ...	2015	26351664
evaluation of the efficacy, potential for vector transmission, and duration of immunity of mp-12, an attenuated rift valley fever virus vaccine candidate, in sheep.	rift valley fever virus (rvfv) causes serious disease in ruminants and humans in africa. in north america, there are susceptible ruminant hosts and competent mosquito vectors, yet there are no fully licensed animal vaccines for this arthropod-borne virus, should it be introduced. studies in sheep and cattle have found the attenuated strain of rvfv, mp-12, to be both safe and efficacious based on early testing, and a 2-year conditional license for use in u.s. livestock has been issued. the purpos ...	2015	26041042
risk factors associated with rift valley fever epidemics in south africa in 2008-11.	rift valley fever (rvf) is a zoonotic and vector-borne disease, mainly present in africa, which represents a threat to human health, animal health and production. south africa has experienced three major rvf epidemics (1950-51, 1973-75 and 2008-11). due to data scarcity, no previous study has quantified risk factors associated with rvf epidemics in animals in south africa. using the 2008-11 epidemic datasets, a retrospective longitudinal study was conducted to identify and quantify spatial and t ...	2015	25804974
molecular identification of saint louis encephalitis virus genotype iv in colombia.	saint louis encephalitis virus (slev) is a member of the japanese-encephalitis virus serocomplex of the genus flavivirus. slev is broadly distributed in the americas and the caribbean islands, where it is usually transmitted by mosquitoes of the genus culex and primarily to birds and mammalian-hosts. humans are occasionally infected by the virus and are dead-end hosts. slev causes encephalitis in temperate regions, while in tropical regions of the americas, several human cases and a wide biologi ...	2015	26313538
temperature-sensitive mutations for live-attenuated rift valley fever vaccines: implications from other rna viruses.	rift valley fever (rvf) is a mosquito-borne zoonotic disease endemic to the african continent. rvf is characterized by high rate of abortions in ruminants and hemorrhagic fever, encephalitis, or blindness in humans. rvf is caused by the rift valley fever virus (rvfv: genus phlebovirus, family bunyaviridae). vaccination is the only known effective strategy to prevent the disease, but there are no licensed rvf vaccines available for humans. a live-attenuated vaccine candidate derived from the wild ...	2015	26322023
the 5' and 3' ends of alphavirus rnas--non-coding is not non-functional.	the non-coding regions found at the 5' and 3' ends of alphavirus genomes regulate viral gene expression, replication, translation and virus-host interactions, which have significant implications for viral evolution, host range, and pathogenesis. the functions of these non-coding regions are mediated by a combination of linear sequence and structural elements. the capped 5' untranslated region (utr) contains promoter elements, translational regulatory sequences that modulate dependence on cellula ...	2015	25630058
vector-host interactions of culiseta melanura in a focus of eastern equine encephalitis virus activity in southeastern virginia.	eastern equine encephalitis virus (eeev) causes a highly pathogenic mosquito-borne zoonosis that is responsible for sporadic outbreaks of severe illness in humans and equines in the eastern usa. culiseta (cs.) melanura is the primary vector of eeev in most geographic regions but its feeding patterns on specific avian and mammalian hosts are largely unknown in the mid-atlantic region. the objectives of our study were to: 1) identify avian hosts of cs. melanura and evaluate their potential role in ...	2015	26327226
tissue barriers to arbovirus infection in mosquitoes.	arthropod-borne viruses (arboviruses) circulate in nature between arthropod vectors and vertebrate hosts. arboviruses often cause devastating diseases in vertebrate hosts, but they typically do not cause significant pathology in their arthropod vectors. following oral acquisition of a viremic bloodmeal from a vertebrate host, the arbovirus disease cycle requires replication in the cellular environment of the arthropod vector. once the vector has become systemically and persistently infected, the ...	2015	26184281
systematic revision of the adeleid haemogregarines, with creation of bartazoon n. g., reassignment of hepatozoon argantis garnham, 1954 to hemolivia, and molecular data on hemolivia stellata.	life cycles and molecular data for terrestrial haemogregarines are reviewed in this article. collection material was re-examined: hepatozoon argantis garnham, 1954 in argas brumpti was reassigned to hemolivia as hemolivia argantis (garnham, 1954) n. comb.; parasite dna was extracted from a tick crush on smear of an archived slide of hemolivia stellata in amblyomma rotondatum, then the 18s ssrrna gene was amplified by pcr. a systematic revision of the group is proposed, based on biological life c ...	2015	26551414
[do some conditions contribute to the reemergence of the venezuelan equine encephalitis virus in the colombian alta guajira?].	in the last 18 years, epizootics of venezuelan equine encephalitis have not occurred in places with historic epidemic register (1925-1995) in the guajira peninsula, colombia.	2015	26148035
a mosquito survey of the twin-island caribbean nation of saint kitts and nevis, 2010.	adult mosquito surveys of saint kitts and nevis (skn) were performed in the dry season (march 16-23, 2010) in saint kitts, and the rainy season (october 18-25, 2010) in skn. biogents (bg) sentinel traps were set with co₂and bg lure in urban, rural, mangrove, and dry forest habitats. mosquitoes were identified to species, and reverse transcription-polymerase chain reaction was performed on potential vector species for dengue virus (denv), chikungunya virus (chikv), and west nile virus (wnv). the ...	2015	26675458
allethrin-based mosquito control device causing knockdown, morbidity, and mortality in four species of field-caught mosquitoes (diptera: culicidae).	a mosquito control device marketed for spatial repellency, the thermacell mosquito repellent appliance, was evaluated in semifield trials against multiple field-caught species of mosquito. using paper and mesh cages, mosquito test groups of at least 30 mosquitoes were suspended in a 2,337 cubic foot outdoor space while two thermacell repellent devices were active. after 30 min of treatment, cages were moved to the laboratory to observe knockdown, morbidity, and mortality for 24 h. species tested ...	2015	26335485
analysis of the genetic variability and structure of ochlerotatus taeniorhynchus (diptera: culicidae) populations from the colombian atlantic coast on the basis of random amplified polymorphic dna markers.	ochlerotatus taeniorhynchus (diptera: culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic venezuelan equine encephalitis in colombia. this study used 9 random amplified polymorphic dna (rapd) markers to analyze the mosquito's genetic variability and genetic structure of 122 specimens in 7 populations from the colombian atlantic coast. assuming that all loci were in hardy-weinberg equilibrium, diversity statistics and analyses were performed. the average ...	2014	24938703
comparing aedes vigilax eggshell densities in saltmarsh and mangrove systems with implications for management.	aedes vigilax (skuse), a nuisance and disease vector, is prolific in intertidal wetlands in australia. aedine mosquitoes oviposit directly onto substrate. the eggshells are relatively stable spatially and temporally, providing an estimate of mosquito larval production. the aims of the research were to compare, at a general level, oviposition in mangroves and saltmarshes, and to compare oviposition between different habitats within mangroves and saltmarshes. the results indicated that there were ...	2014	26462954
effects of photoperiod on the induction of embryonic diapause in aedes taeniorhynchus (diptera: culicidae).		2014	4045935
biological differences between brackish and fresh water-derived aedes aegypti from two locations in the jaffna peninsula of sri lanka and the implications for arboviral disease transmission.	the mainly fresh water arboviral vector aedes aegypti l. (diptera: culicidae) can also undergo pre-imaginal development in brackish water of up to 15 ppt (parts per thousand) salt in coastal areas. we investigated differences in salinity tolerance, egg laying preference, egg hatching and larval development times and resistance to common insecticides in ae. aegypti collected from brackish and fresh water habitats in jaffna, sri lanka. brackish water-derived ae. aegypti were more tolerant of salin ...	2014	25170879
pathogenic landscape of transboundary zoonotic diseases in the mexico-us border along the rio grande.	transboundary zoonotic diseases, several of which are vector borne, can maintain a dynamic focus and have pathogens circulating in geographic regions encircling multiple geopolitical boundaries. global change is intensifying transboundary problems, including the spatial variation of the risk and incidence of zoonotic diseases. the complexity of these challenges can be greater in areas where rivers delineate international boundaries and encompass transitions between ecozones. the rio grande serve ...	2014	25453027
role of micrornas in arbovirus/vector interactions.	the role of micrornas (mirnas) as small non-coding rnas in regulation of gene expression has been recognized. they appear to be involved in regulation of a wide range of cellular pathways that affect several biological processes such as development, the immune system, survival, metabolism and host-pathogen interactions. arthropod-borne viruses impose great economic and health risks around the world. recent advances in mirna biology have shed some light on the role of these small rnas in vector-v ...	2014	25251636
predicting the mosquito species and vertebrate species involved in the theoretical transmission of rift valley fever virus in the united states.	rift valley fever virus (rvfv) is a mosquito-borne virus in the family bunyaviridiae that has spread throughout continental africa to madagascar and the arabian peninsula. the establishment of rvfv in north america would have serious consequences for human and animal health in addition to a significant economic impact on the livestock industry. published and unpublished data on rvfv vector competence, vertebrate host competence, and mosquito feeding patterns from the united states were combined ...	2014	25211133
insect-specific viruses detected in laboratory mosquito colonies and their potential implications for experiments evaluating arbovirus vector competence.	recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. accordingly, we screened our laboratory mo ...	2014	25510714
evans blue as a simple method to discriminate mosquitoes' feeding choice on small laboratory animals.	temperature, humidity, vision, and particularly odor, are external cues that play essential roles to mosquito blood feeding and oviposition. entomological and behavioral studies employ well-established methods to evaluate mosquito attraction or repellency and to identify the source of the blood meal. despite the efficacy of such methods, the costs involved in the production or acquisition of all parts, components and the chemical reagents involved are unaffordable for most researchers from poor ...	2014	25333369
effects of neonicotinoids and fipronil on non-target invertebrates.	we assessed the state of knowledge regarding the effects of large-scale pollution with neonicotinoid insecticides and fipronil on non-target invertebrate species of terrestrial, freshwater and marine environments. a large section of the assessment is dedicated to the state of knowledge on sublethal effects on honeybees (apis mellifera) because this important pollinator is the most studied non-target invertebrate species. lepidoptera (butterflies and moths), lumbricidae (earthworms), apoidae sens ...	2014	25223353
neurophysiological and behavioral responses of gypsy moth larvae to insect repellents: deet, ir3535, and picaridin.	the interactions between insect repellents and the olfactory system have been widely studied, however relatively little is known about the effects of repellents on the gustatory system of insects. in this study, we show that the gustatory receptor neuron (grn) located in the medial styloconic sensilla on the maxillary palps of gypsy moth larvae, and known to be sensitive to feeding deterrents, also responds to the insect repellents deet, ir3535, and picaridin. these repellents did not elicit res ...	2014	24955823
interaction of light regimes and circadian clocks modulate timing of pre-adult developmental events in drosophila.	circadian clocks have been postulated to regulate development time in several species of insects including fruit flies drosophila melanogaster. previously we have reported that selection for faster pre-adult development reduces development time (by ~19 h or ~11%) and clock period (by ~0.5 h), suggesting a role of circadian clocks in the regulation of development time in d. melanogaster. we reasoned that these faster developing flies could serve as a model to study stage-specific interaction of c ...	2014	24885932
dogs, cats, parasites, and humans in brazil: opening the black box.	dogs and cats in brazil serve as primary hosts for a considerable number of parasites, which may affect their health and wellbeing. these may include endoparasites (e.g., protozoa, cestodes, trematodes, and nematodes) and ectoparasites (i.e., fleas, lice, mites, and ticks). while some dog and cat parasites are highly host-specific (e.g., aelurostrongylus abstrusus and felicola subrostratus for cats, and angiostrongylus vasorum and trichodectes canis for dogs), others may easily switch to other h ...	2014	24423244
male aedes aegypti mosquitoes use jh iii transferred during copulation to influence previtellogenic ovary physiology and affect the reproductive output of female mosquitoes.	the effect of male accessory gland substances on female reproductive physiology has been previously described as "activating" egg development. however, no mechanism has been described that can explain how male mosquitoes are able to influence egg development in female mosquitoes. to investigate how male mosquitoes are able to influence ovarian physiology and reproductive output we explored three main questions: (1) do mating and male accessory gland substances affect ovarian physiology and alter ...	2014	24657670
modeling dynamics of culex pipiens complex populations and assessing abatement strategies for west nile virus.	the primary mosquito species associated with underground stormwater systems in the united states are the culex pipiens complex species. this group represents important vectors of west nile virus (wnv) throughout regions of the continental u.s. in this study, we designed a mathematical model and compared it with surveillance data for the cx. pipiens complex collected in beaufort county, south carolina. based on the best fit of the model to the data, we estimated parameters associated with the eff ...	2014	25268229
diversity of mosquitoes and the aquatic insects associated with their oviposition sites along the pacific coast of mexico.	the abundance, richness and diversity of mosquitoes and aquatic insects associated with their oviposition sites were surveyed along eight states of the pacific coast of mexico. diversity was estimated using the shannon index (h'), similarity measures and cluster analysis.	2014	24450800
freshwater biodiversity and aquatic insect diversification.	inland waters cover less than 1% of earth's surface but harbor more than 6% of all insect species: nearly 100,000 species from 12 orders spend one or more life stages in freshwater. little is known about how this remarkable diversity arose, although allopatric speciation and ecological adaptation are thought to be primary mechanisms. freshwater habitats are highly susceptible to environmental change and exhibit marked ecological gradients. standing waters appear to harbor more dispersive species ...	2013	24160433
mosquito-host interactions during and after an outbreak of equine viral encephalitis in eastern panama.	mosquito blood meals provide information about the feeding habits and host preference of potential arthropod-borne disease vectors. although mosquito-borne diseases are ubiquitous in the neotropics, few studies in this region have assessed patterns of mosquito-host interactions, especially during actual disease outbreaks. based on collections made during and after an outbreak of equine viral encephalitis, we identified the source of 338 blood meals from 10 species of mosquitoes from aruza abajo, ...	2013	24339965
dose-response tests and semi-field evaluation of lethal and sub-lethal effects of slow release pyriproxyfen granules (sumilarv®0.5g) for the control of the malaria vectors anopheles gambiae sensu lato.	recently research has shown that larviciding can be an effective tool for integrated malaria vector control. nevertheless, the uptake of this intervention has been hampered by the need to re-apply larvicides frequently. there is a need to explore persistent, environmentally friendly larvicides for malaria vector control to reduce intervention efforts and costs by reducing the frequency of application. in this study, the efficacy of a 0.5% pyriproxyfen granule (surmilarv®0.5g, sumitomo chemicals) ...	2013	23497149
an insight into the sialome of the frog biting fly, corethrella appendiculata.	the nematocera infraorder culicomorpha is believed to have descended from bloodfeeding ancestors over 200 million years ago, generating bloodfeeding and non-bloodfeeding flies in two superfamilies, the culicoidea-containing the mosquitoes, the frog-feeding midges, the chaoboridae, and the dixidae-and the chironomoidea-containing the black flies, the ceratopogonids, the chironomidae, and the thaumaleidae. blood feeding requires many adaptations, including development of a sophisticated salivary p ...	2013	24514880
the risk of rift valley fever virus introduction and establishment in the united states and european union.	rift valley fever virus (rvfv) is an arthropod-borne disease resulting in severe morbidity and mortality in both human and ruminant populations. first identified in kenya in 1930, the geographical range of rvfv has been largely constrained to the african continent, yet has recently spread to new regions, and is identified as a priority disease with potential for geographic emergence. we present a systematic literature review assessing the potential for rvfv introduction and establishment in the ...	2013	26038446
ires-based venezuelan equine encephalitis vaccine candidate elicits protective immunity in mice.	venezuelan equine encephalitis virus (veev) is an arbovirus that causes periodic outbreaks that impact equine and human populations in the americas. one of the veev subtypes located in mexico and central america (ie) has recently been recognized as an important cause of equine disease and death, and human exposure also appears to be widespread. here, we describe the use of an internal ribosome entry site (ires) from encephalomyocarditis virus to stably attenuate veev, creating a vaccine candidat ...	2013	23351391
nature, nurture and evolution of intra-species variation in mosquito arbovirus transmission competence.	mosquitoes vary in their competence or ability to transmit arthropod-borne viruses (arboviruses). many arboviruses cause disease in humans and animals. identifying the environmental and genetic causes of variation in mosquito competence for arboviruses is one of the great challenges in public health. progress identifying genetic (nature) and environmental (nurture) factors influencing mosquito competence for arboviruses is reviewed. there is great complexity in the various traits that comprise m ...	2013	23343982
transmission of pathogens by stomoxys flies (diptera, muscidae): a review.	stomoxys flies are mechanical vectors of pathogens present in the blood and skin of their animal hosts, especially livestock, but occasionally humans. in livestock, their direct effects are disturbance, skin lesions, reduction of food intake, stress, blood loss, and a global immunosuppressive effect. they also induce the gathering of animals for mutual protection; meanwhile they favor development of pathogens in the hosts and their transmission. their indirect effect is the mechanical transmissi ...	2013	23985165
what is the risk for exposure to vector-borne pathogens in united states national parks?	united states national parks attract > 275 million visitors annually and collectively present risk of exposure for staff and visitors to a wide range of arthropod vector species (most notably fleas, mosquitoes, and ticks) and their associated bacterial, protozoan, or viral pathogens. we assessed the current state of knowledge for risk of exposure to vector-borne pathogens in national parks through a review of relevant literature, including internal national park service documents and organismal ...	2013	23540107
zoonotic encephalitides caused by arboviruses: transmission and epidemiology of alphaviruses and flaviviruses.	in this review, we mainly focus on zoonotic encephalitides caused by arthropod-borne viruses (arboviruses) of the families flaviviridae (genus flavivirus) and togaviridae (genus alphavirus) that are important in both humans and domestic animals. specifically, we will focus on alphaviruses (eastern equine encephalitis virus, western equine encephalitis virus, venezuelan equine encephalitis virus) and flaviviruses (japanese encephalitis virus and west nile virus). most of these viruses were origin ...	2013	24427764
rna viruses can hijack vertebrate micrornas to suppress innate immunity.	currently, there is little evidence for a notable role of the vertebrate microrna (mirna) system in the pathogenesis of rna viruses. this is primarily attributed to the ease with which these viruses mutate to disrupt recognition and growth suppression by host mirnas. here we report that the haematopoietic-cell-specific mirna mir-142-3p potently restricts the replication of the mosquito-borne north american eastern equine encephalitis virus in myeloid-lineage cells by binding to sites in the 3' n ...	2013	24352241
vector-borne helminths of dogs and humans in europe.	presently, 45% of the total human population of europe, as well as their domestic and companion animals, are exposed to the risk of vector-borne helminths (vbh) causing diseases. a plethora of intrinsic biological and extrinsic factors affect the relationship among helminths, vectors and animal hosts, in a constantly changing environment. although canine dirofilarioses by dirofilaria immitis and dirofilaria repens are key examples of the success of vbh spreading into non-endemic areas, another e ...	2013	23324440
[seasonal and time dynamics of aedes aegypti (diptera: culicidae) in cienfuegos municipality].	dengue is one of the most important vector-borne diseases in las americas due, among other factors, to the increase of aedes aegypti populations.	2013	23431620
secretion of water and ions by malpighian tubules of larval mosquitoes: effects of diuretic factors, second messengers, and salinity.	the effects of changes in the salinity of the rearing medium on malpighian tubule fluid secretion and ion transport were examined in larvae of the freshwater mosquito aedes aegypti and the saltwater species ochlerotatus taeniorhynchus. for unstimulated tubules of both species, the k(+) concentration of secreted fluid was significantly lower when larvae were reared in 30% or 100% seawater (o. taeniorhynchus only), relative to tubules from freshwater-reared larvae. the na(+) concentration of secre ...	2013	16691529
host selection and parasite infection in aedes taeniorhynchus, endemic disease vector in the galápagos islands.	host selection in blood-sucking arthropods has important evolutionary and ecological implications for the transmission dynamics, distribution and host-specificity of the parasites they transmit. the black salt-marsh mosquito (aedes taeniorhynchus wiedemann) is distributed throughout tropical to temperate coastal zones in the americas, and continental populations are primarily mammalphilic. it is the only indigenous mosquito in the galápagos islands, having colonised the archipelago around 200,00 ...	2012	22921730
field evaluation of the off! clip-on mosquito repellent (metofluthrin) against aedes albopictus and aedes taeniorhynchus (diptera: culicidae) in northeastern florida.	repellent efficacy of the off! clip-on mosquito repellent device (s. c. johnson and son, inc., racine, wi) containing metofluthrin was evaluated on six human volunteers against the container-breeding mosquito aedes albopictus (skuse) and the salt marsh mosquito aedes taeniorhynchus (wiedemann) at two field locations in northeastern florida. the device repelled mosquitoes by releasing a vaporized form of the pyrethroid insecticide metofluthrin ([ai] 31.2%) and provided 70% protection from ae. alb ...	2012	22679874
the impact of industrial anthropization on mosquito (diptera, culicidae) communities in mangrove areas of guanabara bay (rio de janeiro, brazil).	the effects of industrial anthropization on species composition and community diversity of culicidae (diptera) were studied in a mangrove area impacted by industrial activities as compared to a preserved area, both around guanabara bay in the state of rio de janeiro, brazil. diversity, equitability, and species richness in culicidae community differed between the studied areas. indicator species analysis and correspondence analysis were carried out and indicated that the sabethini, especially wy ...	2012	23950012
global climate change and its potential impact on disease transmission by salinity-tolerant mosquito vectors in coastal zones.	global climate change can potentially increase the transmission of mosquito vector-borne diseases such as malaria, lymphatic filariasis, and dengue in many parts of the world. these predictions are based on the effects of changing temperature, rainfall, and humidity on mosquito breeding and survival, the more rapid development of ingested pathogens in mosquitoes and the more frequent blood feeds at moderately higher ambient temperatures. an expansion of saline and brackish water bodies (water wi ...	2012	22723781
genetic and anatomic determinants of enzootic venezuelan equine encephalitis virus infection of culex (melanoconion) taeniopus.	venezuelan equine encephalitis (vee) is a re-emerging, mosquito-borne viral disease with the potential to cause fatal encephalitis in both humans and equids. recently, detection of endemic vee caused by enzootic strains has escalated in mexico, peru, bolivia, colombia and ecuador, emphasizing the importance of understanding the enzootic transmission cycle of the etiologic agent, vee virus (veev). the majority of work examining the viral determinants of vector infection has been performed in the ...	2012	22509419
quantification of intrahost bottlenecks of west nile virus in culex pipiens mosquitoes using an artificial mutant swarm.	mosquito-borne viruses are predominantly rna viruses which exist within hosts as diverse mutant swarms. defining the way in which stochastic forces within mosquito vectors shape these swarms is critical to advancing our understanding of the evolutionary and adaptive potential of these pathogens. there are multiple barriers within a mosquito which a viral swarm must traverse in order to ultimately be transmitted. here, using artificial mutant swarms composed of neutral variants of west nile virus ...	2012	22326536
giant viruses coexisted with the cellular ancestors and represent a distinct supergroup along with superkingdoms archaea, bacteria and eukarya.	the discovery of giant viruses with genome and physical size comparable to cellular organisms, remnants of protein translation machinery and virus-specific parasites (virophages) have raised intriguing questions about their origin. evidence advocates for their inclusion into global phylogenomic studies and their consideration as a distinct and ancient form of life.	2012	22920653
twenty-four years in the mud: what have we learned about the natural history and ecology of the mangrove rivulus, kryptolebias marmoratus?	although first described in 1880, kryptolebias marmoratus avoided scientific scrutiny until 1961, when it was identified as the only known selfing hermaphroditic vertebrate. the subsequent intense interest in this fish as a laboratory animal, continuing to this day, might explain the paucity of wild collections, but our collective knowledge now suggests that the inherent difficulty of wild collection is more a matter of "looking in all the wrong places." long thought to be rare in the mangroves, ...	2012	22576816
north american wetlands and mosquito control.	wetlands are valuable habitats that provide important social, economic, and ecological services such as flood control, water quality improvement, carbon sequestration, pollutant removal, and primary/secondary production export to terrestrial and aquatic food chains. there is disagreement about the need for mosquito control in wetlands and about the techniques utilized for mosquito abatement and their impacts upon wetlands ecosystems. mosquito control in wetlands is a complex issue influenced by ...	2012	23222252
ovary ecdysteroidogenic hormone activates egg maturation in the mosquito georgecraigius atropalpus after adult eclosion or a blood meal.	the rockpool mosquito, georgecraigius atropalpus, is a facultatively autogenous species that produces its first egg clutch without a blood meal shortly after emergence. several days after depositing this clutch, females must take a blood meal to produce a second egg clutch. decapitation of females shortly after emergence or blood ingestion prevents egg maturation. here, we report that a single injected dose of the neuropeptide ovary ecdysteroidogenic hormone (oeh) fully restored egg maturation i ...	2012	22811249
population genetic structure of aedes polynesiensis in the society islands of french polynesia: implications for control using a wolbachia-based autocidal strategy.	aedes polynesiensis is the primary vector of wuchereria bancrofti in the south pacific and an important vector of dengue virus. an improved understanding of the mosquito population genetics is needed for insight into the population dynamics and dispersal, which can aid in understanding the epidemiology of disease transmission and control of the vector. in light of the potential release of a wolbachia infected strain for vector control, our objectives were to investigate the microgeographical and ...	2012	22531297
effect of water quality on the efficacy of water-base suspensions of arosurf msf against larvae of aedes taeniorhynchus: bioassay evaluations.		2012	3504951
110 years of avipoxvirus in the galapagos islands.	the role of disease in regulating populations is controversial, partly owing to the absence of good disease records in historic wildlife populations. we examined birds collected in the galapagos islands between 1891 and 1906 that are currently held at the california academy of sciences and the zoologisches staatssammlung muenchen, including 3973 specimens representing species from two well-studied families of endemic passerine birds: finches and mockingbirds. beginning with samples collected in ...	2011	21249151
isolation of novel bacillus species showing high mosquitocidal activity against several mosquito species.	two novel mosquitocidal bacteria, vb17 and vb24, identified as new bacillus species were isolated from dead mosquito larvae obtained in florida aquatic habitats. gas chromatographic analysis of fatty acid methyl esters (gc-fame) and 16s rrna sequencing indicated that vb24 is closely related to bacillus sphaericus whereas vb17 does not have a close relationship with either bacillus thuringiensis or b. sphaericus. both isolates were significantly more active than b. sphaericus 2362 against aedes t ...	2011	21276795
mosquito infestation and dengue virus infection in aedes aegypti females in schools in merida, mexico.	we determined abundance of aedes aegypti mosquitoes and presence of dengue virus (denv) in females collected from schools in mérida, méxico, during 2008 and 2009. backpack aspiration from 24 schools produced 468 females of ae. aegypti and 1,676 females of another human biter, culex quinquefasciatus. ae. aegypti females were collected most commonly from classrooms followed by offices and bathrooms. of these females, 24.7% were freshly fed. examination of 118 pools of ae. aegypti females (total of ...	2011	21363990
the midgut transcriptome of phlebotomus (larroussius) perniciosus, a vector of leishmania infantum: comparison of sugar fed and blood fed sand flies.	abstract:	2011	21569254
laboratory selection for an accelerated mosquito sexual development rate.	abstract: background: separating males and females at the early adult stage did not ensure the virginity of females of anopheles arabiensis (dongola laboratory strain), whereas two years earlier this method had been successful. in most mosquito species, newly emerged males and females are not able to mate successfully. for anopheline species, a period of 24 h post-emergence is generally required for the completion of sexual maturation, which in males includes a 180degrees rotation of the genital ...	2011	21595988
implications of plasmodium parasite infected mosquitoes on an insular avifauna: the case of socorro island, méxico.	avian malaria (plasmodium spp.) has been implicated in the decline of avian populations in the hawaiian islands and it is generally agreed that geographically isolated and immunologically naïve bird populations are particularly vulnerable to the pathogenic effects of invasive malaria parasites. in order to assess the potential disease risk of malaria to the avifauna of socorro island, méxico, we surveyed for plasmodium isolates from 1,300 resident field-caught mosquitoes. most of them were ident ...	2011	21635660
mosquito (diptera: culicidae) bloodmeal sources during a period of west nile virus transmission in puerto rico.	host bloodmeals of indigenous caribbean mosquitoes have not been studied previously. we identified vertebrate dna in 90 blood-engorged mosquitoes belonging to four genera (aedes, culex, deinocerites, and uranotaenia) and 12 species that were collected in puerto rico within a geographic and temporal focus of west nile virus transmission in 2007. it was found that 62 (68.8%) bloodmeals were from reptiles, 18 (20.0%) from birds, and 10 (11.1%) from mammals. only one bloodmeal of 18 derived from cul ...	2011	21661334
endemic venezuelan equine encephalitis in the americas: hidden under the dengue umbrella.	venezuelan equine encephalitis (vee) is an emerging infectious disease in latin america. outbreaks have been recorded for decades in countries with enzootic circulation, and the recent implementation of surveillance systems has allowed the detection of additional human cases in countries and areas with previously unknown vee activity. clinically, vee is indistinguishable from dengue and other arboviral diseases and confirmatory diagnosis requires the use of specialized laboratory tests that are ...	2011	21765860
susceptibility of mosquito and lepidopteran cell lines to the mosquito iridescent virus (iiv-3) from aedes taeniorhynchus.	mosquito iridescent viruses (miv) are members of the genus chloriridovirus that currently contains only the type iiv-3 from aedestaeniorhynchus. the complete genome of invertebrate iridescent virus -3 (iiv-3) has been sequenced and the availability of a tissue culture system would facilitate functional genomic studies. this investigation, using quantitative pcr and electron microscopy, has determined that the mosquito cell lines aedes aegypti (aag2), aedes albopictus (c6/36) and anopheles gambia ...	2011	21741386
the fat body transcriptomes of the yellow fever mosquito aedes aegypti, pre- and post- blood meal.	the fat body is the main organ of intermediary metabolism in insects and the principal source of hemolymph proteins. as part of our ongoing efforts to understand mosquito fat body physiology and to identify novel targets for insect control, we have conducted a transcriptome analysis of the fat body of aedes aegypti before and in response to blood feeding.	2011	21818341
adaptation, isolation by distance and human-mediated transport determine patterns of gene flow among populations of the disease vector aedes taeniorhynchus in the galapagos islands.	the black salt-marsh mosquito (aedes taeniorhynchus) is the only native mosquito in the galapagos islands and potentially a major disease vector for galapagos wildlife. little is known about its population structure, or how its dynamics may be influenced by human presence in the archipelago. we used microsatellite data to assess the structure and patterns of a. taeniorhynchus gene flow among and within islands, to identify potential barriers to mosquito dispersal, and to investigate human-aided ...	2011	21968211
Population Dynamics of Aedes aegypti and Dengue as Influenced by Weather and Human Behavior in San Juan, Puerto Rico.	Previous studies on the influence of weather on Aedes aegypti dynamics in Puerto Rico suggested that rainfall was a significant driver of immature mosquito populations and dengue incidence, but mostly in the drier areas of the island. We conducted a longitudinal study of Ae. aegypti in two neighborhoods of the metropolitan area of San Juan city, Puerto Rico where rainfall is more uniformly distributed throughout the year. We assessed the impacts of rainfall, temperature, and human activities on ...	2011	22206021
candidate vectors and rodent hosts of venezuelan equine encephalitis virus, chiapas, 2006-2007.	enzootic venezuelan equine encephalitis virus (veev) has been known to occur in mexico since the 1960s. the first natural equine epizootic was recognized in chiapas in 1993 and since then, numerous studies have characterized the etiologic strains, including reverse genetic studies that incriminated a specific mutation that enhanced infection of epizootic mosquito vectors. the aim of this study was to determine the mosquito and rodent species involved in enzootic maintenance of subtype ie veev in ...	2011	22144461
west nile virus vector competency of culex quinquefasciatus mosquitoes in the galapagos islands.	the mosquito-transmitted pathogen west nile virus (wnv) is not yet present in the galápagos archipelago of ecuador. however, concern exists for fragile endemic island fauna after population decreases in several north american bird species and pathology in certain reptiles. we examined wnv vector competency of a galápagos strain of mosquito (culex quinquefasciatus say). field specimens were tested for their capacity to transmit the wn02-1956 strain of wnv after incubation at 27°c or 30°c. rates o ...	2011	21896799
West Nile virus genetic diversity is maintained during transmission by Culex pipiens quinquefasciatus mosquitoes.	Due to error-prone replication, RNA viruses exist within hosts as a heterogeneous population of non-identical, but related viral variants. These populations may undergo bottlenecks during transmission that stochastically reduce variability leading to fitness declines. Such bottlenecks have been documented for several single-host RNA viruses, but their role in the population biology of obligate two-host viruses such as arthropod-borne viruses (arboviruses) in vivo is unclear, but of central impor ...	2011	21935412
experimental transmission of mayaro virus by aedes aegypti.	outbreaks of mayaro fever have been associated with a sylvatic cycle of mayaro virus (mayv) transmission in south america. to evaluate the potential for a common urban mosquito to transmit mayv, laboratory vector competence studies were performed with aedes aegypti from iquitos, peru. oral infection in ae. aegypti ranged from 0% (0/31) to 84% (31/37), with blood meal virus titers between 3.4 log(10) and 7.3 log(10) plaque-forming units (pfu)/ml. transmission of mayv by 70% (21/30) of infected mo ...	2011	21976583
conditioning individual mosquitoes to an odor: sex, source, and time.	olfactory conditioning of mosquitoes may have important implications for vector-pathogen-host dynamics. if mosquitoes learn about specific host attributes associated with pathogen infection, it may help to explain the heterogeneity of biting and disease patterns observed in the field. sugar-feeding is a requirement for survival in both male and female mosquitoes. it provides a starting point for learning research in mosquitoes that avoids the confounding factors associated with the observer bein ...	2011	21887384
co-occurrence patterns of the dengue vector aedes aegypti and aedes mediovitattus, a dengue competent mosquito in puerto rico.	aedes aegypti is implicated in dengue transmission in tropical and subtropical urban areas around the world. ae. aegypti populations are controlled through integrative vector management. however, the efficacy of vector control may be undermined by the presence of alternative, competent species. in puerto rico, a native mosquito, ae. mediovittatus, is a competent dengue vector in laboratory settings and spatially overlaps with ae. aegypti. it has been proposed that ae. mediovittatus may act as a ...	2011	21989642
genetic analysis of scattered populations of the indian eri silkworm, samia cynthia ricini donovan: differentiation of subpopulations.	deforestation and exploitation has led to the fragmentation of habitats and scattering of populations of the economically important eri silkworm, samia cynthia ricini, in north-east india. genetic analysis of 15 eri populations, using issr markers, showed 98% inter-population, and 23% to 58% intra-population polymorphism. nei's genetic distance between populations increased significantly with altitude (r(2) = 0.71) and geographic distance (r(2) = 0.78). on the dendrogram, the lower and upper ass ...	2011	21931526
the giant cafeteria roenbergensis virus that infects a widespread marine phagocytic protist is a new member of the fourth domain of life.	a recent work has provided strong arguments in favor of a fourth domain of life composed of nucleo-cytoplasmic large dna viruses (ncldvs). this hypothesis was supported by phylogenetic and phyletic analyses based on a common set of proteins conserved in eukarya, archaea, bacteria, and viruses, and implicated in the functions of information storage and processing. recently, the genome of a new ncldv, cafeteria roenbergensis virus (crov), was released. the present work aimed to determine if crov s ...	2011	21559486
susceptibility of adult mosquitoes to insecticides in aqueous sucrose baits.	mosquitoes characteristically feed on plant-derived carbohydrates and honeydew just after emergence and intermittently during their lives. development of toxic baits focusing on this carbohydrate-seeking behavior may potentially contribute to localized control. in the present study, ten insecticides were fed to female culex quinquefasciatus, anopheles quadrimaculatus, and aedes taeniorhynchus in a 10% sucrose solution. active ingredients representative of five classes of insecticides (pyrethroid ...	2011	21635642
identification and transcription profiling of trypsin in aedes taeniorhynchus (diptera: culicidae): developmental regulation, blood feeding, and permethrin exposure.	the cdna of a trypsin gene from aedes (ochlerotatus) taeniorhynchus (weidemann) was cloned and sequenced. the full-length mrna sequence (890 bp) for trypsin from ae. taeniorhynchus (aettryp1) was obtained, which encodes an open reading frame of 765 bp (i.e., 255 amino acids). to detect whether aettryp is developmentally regulated, a quantitative real-time polymerase chain reaction was used to examine aettrypl mrna expression levels in different developmental stages of ae. taeniorhynchus. aettryp ...	2011	21661315
first record of anopheles albimanus from st kitts.	previous mosquito surveys performed in the federation of st kitts and nevis identified anopheles albimanus in nevis but there is no recorded occurrence of this mosquito in st kitts. to determine the presence of this and other species in st kitts and nevis, a mosquito survey was conducted.	2011	22519234
aerial ultra-low-volume application of naled: impact on nontarget imperiled butterfly larvae (cyclargus thomasi bethunebakeri) and efficacy against adult mosquitoes (aedes taeniorhynchus).	we assessed the exposure and acute toxicity of naled, applied aerially as an ultra-low-volume spray for mosquito control, on late instar larvae of the miami blue (cyclargus thomasi bethunebakeri) (comstock and huntington 1943) (lepidoptera: lycaenidae), an imperiled south florida butterfly. we concurrently evaluated the control efficacy against caged adult female salt-marsh mosquitoes (aedes taeniorhynchus) (wiedemann 1821) (diptera: culicidae). this 3-yr study was conducted in north key largo ( ...	2010	22182563