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antimicrobial efficacy and safety of mucoadhesive exopolymer produced by acinetobacter haemolyticus.this study evaluated five extracellular polymers of bacterial origin possessing mucoadhesive properties for their antimicrobial properties and toxicological characteristics. of the five tested mucoadhesive biopolymers, the extracellular polymer produced by a strain of acinetobacter haemolyticus exhibited broad antimicrobial efficacy towards yersinia enterocolitica, salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7 and bacillus subtilis. significant (p<0.05) inhibition of g ...201727720965
the role of universal stress proteins in bacterial infections.universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. a general property of usps is their role in adaptation of bacteria to oxidative stress, high temperature, low ph and/or hypoxia. there is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. usps in mycobacterium tuberculosis are well studied and may play a role in latency o ...201728120710
sumoylation as an integral mechanism in bacterial infection and disease progression.post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. among these, particularly the modification with small ubiquitin-like modifiers (sumo) is being recognized as a pathway crucial for cell homeostasis and health. understandably, bacterial pathogens intervene with the sumo pathway of the host for ensuring successful infection. among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. majo ...201728197924
rational design of reversible inhibitors for trehalose 6-phosphate phosphatases.in some organisms, environmental stress triggers trehalose biosynthesis that is catalyzed collectively by trehalose 6-phosphate synthase, and trehalose 6-phosphate phosphatase (t6pp). t6pp catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to trehalose and inorganic phosphate and is a promising target for the development of antibacterial, antifungal and antihelminthic therapeutics. herein, we report the design, synthesis and evaluation of a library of aryl d-glucopyranoside 6-sulfates to se ...201728192710
design, synthesis and spectroscopic characterization of metal (ii) complexes derived from a tetradentate macrocyclic ligand: study on antimicrobial and antioxidant capacity of complexes.the paper presents the synthesis of co(ii), ni(ii) and cu(ii) complexes of macrocyclic schiff base ligand derived from 1, 4-dicarbonyl-phenyl-dihydrazide and ethyl 3-oxobutanoate (2:2). the synthesized ligand and its metal complexes were characterized by elemental analyses, magnetic susceptibility measurements, ftir, uv-vis., mass (1)h nmr and x-ray diffraction. the cu(ii) complex exhibit distorted octahedral geometry, whereas an octahedral geometry is suggested for other complexes. the synthesi ...201728126666
flexible thin-layer plasma inactivation of bacteria and mold survival in beef jerky packaging and its effects on the meat's physicochemical properties.the aims of the present study were to examine the use of a flexible thin-layer plasma system in inactivating bacteria and mold on beef jerky in a commercial package and to evaluate the physicochemical changes of the jerky. after plasma treatment for 10min, escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and aspergillus flavus populations on the beef jerky were reduced by approximately 2 to 3log cfu/g. no significant changes in metmyoglobin content, shear force, and myof ...201727718408
differential roles of caspase-1 and caspase-11 in infection and inflammation.caspase-1, also known as interleukin-1β (il-1β)-converting enzyme (ice), regulates antimicrobial host defense, tissue repair, tumorigenesis, metabolism and membrane biogenesis. on activation within an inflammasome complex, caspase-1 induces pyroptosis and converts pro-il-1β and pro-il-18 into their biologically active forms. "ice(-/-)" or "casp1(-/-)" mice generated using 129 embryonic stem cells carry a 129-associated inactivating passenger mutation on the caspase-11 locus, essentially making t ...201728345580
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
effects of β-caryophyllene and murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time-kill curve studies.orange jessamine [murraya paniculata l. (rutaceae)] has been used worldwide in folk medicine as an anti-inflammatory, antibiotic and analgesic.201727927082
active edible coating and γ-irradiation as cold combined treatments to assure the safety of broccoli florets (brassica oleracea l.).the antimicrobial activity of essential oils (eos), organic acid (oa) salts and natamycin, a natural antifungal produced during fermentation by the bacterium streptomyces natalensis, was assessed against four pathogens (escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium and aspergillus niger). the minimum inhibitory concentration (mic) of each antimicrobial (am) was assessed to determine their efficiency on tested microbial species in order to select the most efficient. the ...201727744210
antimicrobial properties of black grape (vitis vinifera l.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds.black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. the purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively.201726729960
cellulase-assisted extraction of polysaccharides from malva sylvestris: process optimization and potential functionalities.enzyme-assisted extraction process of the water-soluble malva sylvestris polysaccharides (msps) was optimized using response surface methodology (rsm). the highest yield (10.40%) of msps was achieved at 5.64% cellulase, 55.65°c temperature, 3.4h time, and 5.22 ph. three homogeneous polysaccharide fractions (msp-1, msp-2, msp-3) were purified by deae-cellulose and sephadex g-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar r ...201728327424
antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects.silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. these nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. however, few studies have investigated the use of industrial waste materials in nan ...201728261161
the antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts.the development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. we were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. we decided to work on extracts of nine plant species with good activity against escherichia coli based on earlier work in the phyto ...201728241818
inactivation and changes in metabolic profile of selected foodborne bacteria by 460 nm led illumination.the objective of this study was to investigate the effect of 460 nm light-emitting diode (led) on the inactivation of foodborne bacteria. additionally, the change in the endogenous metabolic profile of led illuminated cells was analyzed to understand the bacterial response to the led illumination. six different species of bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium) were illuminated with 460 ...201728040158
cmc stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics.in the present study silver nanoparticles were synthesized by reduction of agno3 using aqueous cmc solution, which acts as both reducing and capping agent. the formation of agno3 nanoparticles was observed visually by color change and these nanoparticles were characterized through uv-vis spectroscopy, ftir, xrd, sem, eds and afm. the ftir peaks observed to be ranging from 3300 to 605cm(-1). the afm image clearly showed the surface morphology of well dispersed nanoparticles. sem image illustrates ...201728024537
chemical composition and in vitro evaluation of antimicrobial, antioxidant and antigerminative properties of the seed oil from the tunisian endemic ferula tunetana pomel ex batt.essential oil of the seeds from the endemic tunisian plant ferula tunetana pomel ex batt. was analyzed for its chemical composition and screened for its antimicrobial, antioxidant and antigerminative properties. the chemical composition of the isolated oil is reported for the first time. according to the gc/fid, gc/ms and (13) c-nmr analysis results; 18 compounds were identified accounting for 84.6% of the total oil. the chemical composition of this essential oil was characterized by the presenc ...201727638478
construction of bacillus subtilis strain engineered for expression of porcine β-defensin-2/cecropin p1 fusion antimicrobial peptides and its growth-promoting effect and antimicrobial activity.to generate recombinant bacillus subtilis (b. subtilis) engineered for expression of porcine β-defensin-2 (pbd-2) and cecropin p1 (cp1) fusion antimicrobial peptide and investigate their anti-bacterial activity in vitro and their growth-promoting and disease resisting activity in vivo.201727383796
preliminary assessment on potentials of probiotic b. subtilis rx7 and b. methylotrophicus c14 strains as an immune modulator in salmonella-challenged weaned pigs.a total of 40 crossbred weaned piglets (28 days old; [landrace × yorkshire] × duroc) were used for preliminary assessment on potentials of bacillus-based probiotics as an immune modulator in a salmonella typhimurium challenge model in a 3-week experiment. pigs were randomly allotted to four experimental diets according to their initial body weight (9.21 ± 1.1 kg) and sex (10 pigs per treatment; 5 barrows and 5 gilts). the dietary treatments were basal diet (con), basal diet + oral administration ...201728326485
inactivation of gram-positive bacteria by novel phenolic branched-chain fatty acids.novel phenolic branched-chain fatty acids (pbc-fas) were evaluated for their antimicrobial properties against both gram-positive ( listeria innocua , bacillus subtilis , enterococcus faecium ) and gram-negative ( escherichia coli , salmonella typhimurium, and pseudomonas tolaasii ) bacteria. in addition, pbc-fa derivatives, such as pbc-fa methyl ester mixture, methyl-branched fatty acid mixtures, and trimethylsilyl-pbc-fa methyl esters, were synthesized to study the structure activity relationsh ...201728221889
respiration of microbiota-derived 1,2-propanediol drives salmonella expansion during colitis.intestinal inflammation caused by salmonella enterica serovar typhimurium increases the availability of electron acceptors that fuel a respiratory growth of the pathogen in the intestinal lumen. here we show that one of the carbon sources driving this respiratory expansion in the mouse model is 1,2-propanediol, a microbial fermentation product. 1,2-propanediol utilization required intestinal inflammation induced by virulence factors of the pathogen. s. typhimurium used both aerobic and anaerobic ...201728056091
u-omp19 from brucella abortus is a useful adjuvant for vaccine formulations against salmonella infection in mice.most pathogens infect through mucosal surfaces, and parenteral immunization typically fails to induce effective immune responses at these sites. development of oral-administered vaccines capable of inducing mucosal as well as systemic immunity while bypassing the issues of antigen degradation and immune tolerance could be crucial for the control of enteropathogens. this study demonstrates that u-omp19, a bacterial protease inhibitor with immunostimulatory features, coadministered with salmonella ...201728261222
human α-defensin 6: a small peptide that self-assembles and protects the host by entangling microbes.human α-defensin 6 (hd6) is a 32-residue cysteine-rich peptide that contributes to innate immunity by protecting the host at mucosal sites. this peptide is produced in small intestinal paneth cells, stored as an 81-residue precursor peptide named prohd6 in granules, and released into the lumen. one unusual feature of hd6 is that it lacks the broad-spectrum antimicrobial activity observed for other human α-defensins, including the paneth cell peptide human α-defensin 5 (hd5). hd6 exhibits unprece ...201728296382
variable coordination and c-s bond cleavage activity of n-substituted imidazolidine-2-thiones towards copper: synthesis, spectroscopy, structures, esi-mass and antimicrobial studies.an equimolar reaction of copper(i) iodide with n-ethyl-imidazolidine-2-thione (l-et) in acetonitrile formed black prismatic crystals over a period of three weeks. the x-ray structure determination of black crystals revealed that the thio-ligand l-et has transformed into a new thio-ligand, 1-ethyl-3-(1-ethyl-4,5-dihydro-1h-imidazol-2-yl)imidazolidine-2-thione (l-net), through c-s rupture/c-n bond formation which was coordinated in the unusual 2d polymer, {(cu(ii)(κ(2)-n,s-l-net)2)·(cui4)·(cui2)}n ...201728070570
antibacterial activities of ankaferd hemostat (abs) on shiga toxin-producing escherichia coli and other pathogens significant in foodborne diseases.ankaferd hemostat (ankaferd blood stopper®, abs)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. topical endoscopic abs application is effective in the controlling of gastrointestinal (gi) system hemorrhages and/or infected gi wounds. escherichia coli o157:h7, the predominant serotype of enterohemorrhagic e. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. the aim of this study i ...201726377624
antimicrobial activities of phenolic extracts derived from seed coats of selected soybean varieties.soybean hulls or seed coats consist of complex carbohydrates, proteins, lipids, and polyphenols such as anthocyanidins, proanthocyanidins, and isoflavones. the polyphenolics in the seed coats give them various colors such as black, brown, green, yellow, or even a mottled appearance. in this study, the antimicrobial effects of phenolic extracts from the seed coats of different colored soybeans (yellow, dark brown, brown, and black) were evaluated against foodborne pathogens such as salmonella typ ...201728178372
mplex: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling.the continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. molecular characterization of infectious samples outside of appropriate biosafety containment can take place only subsequent to pathogen inactivation. herein, we describe a modified folch extraction using chloroform/methanol that facilita ...201728091625
antimicrobial resistance of salmonella serovars and campylobacter spp. isolated from an opportunistic gull species, yellow-legged gull ( larus michahellis ).wildlife is a natural reservoir of salmonella and campylobacter, the most important human foodborne pathogens worldwide. free-living birds have the potential to transport, over large distances, such zoonotic bacteria that may harbor antimicrobial resistance traits. on the northeastern iberian coast, we assessed the role of yellow-legged gulls ( larus michahellis ) as reservoirs of antimicrobial resistance in salmonella and thermophilic campylobacter isolates recovered from gulls at three colonie ...201727723383
interferon-inducible guanylate-binding proteins at the interface of cell-autonomous immunity and inflammasome activation.guanylate-binding proteins (gbps) are essential components of cell-autonomous immunity. in response to ifn signaling, gbps are expressed in the cytoplasm of immune and nonimmune cells, where they unleash their antimicrobial activity toward intracellular bacteria, viruses, and parasites. recent studies have revealed that gbps are essential for mediating activation of the caspase-1 inflammasome in response to the gram-negative bacteria salmonella enterica serovar typhimurium, francisella novicida, ...201727418355
star anise (illicium verum hook. f.) as quorum sensing and biofilm formation inhibitor on foodborne bacteria: study in milk.bacteria use quorum sensing (qs) systems to communicate with each other and regulate microbial group behavior, such as the secretion of virulence factors, including biofilm formation. in order to explore safe, edible agents, the potential of star anise (sa) as an anti-qs and antibiofilm agent and its possible application in milk safety were investigated. staphylococcus aureus , salmonella typhimurium, pseudomonas aeruginosa , and biosensor strain chromobacterium violaceum were selected as test s ...201728294685
anti-quorum sensing and anti-biofilm activity of amomum tsaoko (amommum tsao-ko crevost et lemarie) on foodborne pathogens.cell-to-cell communication or quorum sensing (qs) leads to biofilm formation and causing other virulence factors which are extreme problems for food safety, biofilm related infectious diseases etc. this study evaluated the anti-qs activity of the amomum tsaoko extract (0.5-4 mg/ml) by using chromobacterium violaceum a biosensor strain and biofilm formation by crystal violate assay. experimental results demonstrated that the overall yield of amomum tsao-ko extract was 11.33 ± 0.3% (w/w). mic for ...201728149169
bile acid administration elicits an intestinal antimicrobial program and reduces the bacterial burden in two mouse models of enteric infection.in addition to their chemical antimicrobial nature, bile acids (ba) are thought to have other functions in the homeostatic control of gastrointestinal immunity. however, those functions have remained largely undefined. in this work, we used ileal explants and mouse models of ba administration to investigate the role of ba in the regulation of the intestinal antimicrobial response. mice fed on a diet supplemented with 0.1% chenodeoxycholic acid (cdca) showed an upregulated expression of paneth ce ...201728348052
sensory neuron regulation of gastrointestinal inflammation and bacterial host defence.sensory neurons in the gastrointestinal tract have multifaceted roles in maintaining homeostasis, detecting danger and initiating protective responses. the gastrointestinal tract is innervated by three types of sensory neurons: dorsal root ganglia, nodose/jugular ganglia and intrinsic primary afferent neurons. here, we examine how these distinct sensory neurons and their signal transducers participate in regulating gastrointestinal inflammation and host defence. sensory neurons are equipped with ...201728155242
identification of novel host interactors of effectors secreted by salmonella and citrobacter.many pathogenic bacteria of the family enterobacteriaceae use type iii secretion systems to inject virulence proteins, termed "effectors," into the host cell cytosol. although host-cellular activities of several effectors have been demonstrated, the function and host-targeted pathways of most of the effectors identified to date are largely undetermined. to gain insight into host proteins targeted by bacterial effectors, we performed coaffinity purification of host proteins from cell lysates usin ...201727822540
targeting and alteration of tight junctions by bacteria and their virulence factors such as clostridium perfringens enterotoxin.the integrity of tight junctions, which regulate paracellular permeability, is challenged by many bacterial pathogens. this is caused by inflammatory responses triggered by pathogens and direct interaction of bacteria or their toxins with host epithelial cells. in some cases, tight junction proteins represent receptors for cell surface proteins or toxins of the pathogen, such as clostridium perfringens enterotoxin (cpe). cpe causes diarrhea and cramps-the symptoms of a common foodborne illness, ...201727864644
robust growth of avirulent phase ii coxiella burnetii in bone marrow-derived murine macrophages.published data show that murine bone marrow-derived macrophages (bmdm) restrict growth of avirulent phase ii, but not virulent phase i, coxiella burnetii. growth restriction of phase ii bacteria is thought to result from potentiated recognition of pathogen-associated molecular patterns, which leads to production of inhibitory effector molecules. past studies have used conditioned medium from l-929 murine fibroblasts as a source of macrophage-colony stimulating factor (m-csf) to promote different ...201728278296
drying parameters greatly affect the destruction of cronobacter sakazakii and salmonella typhimurium in standard buffer and milk.salmonella typhimurium and cronobacter sakazakii are two foodborne pathogens involved in neonatal infections from milk powder and infant formula. their ability to survive in low-moisture food and during processing from the decontamination to the dried state is a major issue in food protection. in this work, we studied the effects of the drying process on salmonella typhimurium and cronobacter sakazakii, with the aim of identifying the drying parameters that could promote greater inactivation of ...201727889170
probiotic properties and adsorption of enterococcus faecalis psct3-7 to vermiculite.the probiotic properties of enterococcus (e.) faecalis psct3-7, a new strain isolated from the intestines of pigs fed dietary fiber containing 50% sawdust, were investigated. e. faecalis psct3-7 tolerated a ph range of 3 to 8 and 0.3% bile salts, and it inhibited the growth of salmonella typhimurium in a concentration-dependent manner. in addition, e. faecalis showed resistance to several antibacterial agents. vermiculite, a nutrient and microbial carrier, increased the bile tolerance of the str ...201727456777
mechanism of action of electrospun chitosan-based nanofibers against meat spoilage and pathogenic bacteria.this study investigates the antibacterial mechanism of action of electrospun chitosan-based nanofibers (cnfs), against escherichia coli, salmonella enterica serovar typhimurium, staphylococcus aureus and listeria innocua, bacteria frequently involved in food contamination and spoilage. cnfs were prepared by electrospinning of chitosan and poly(ethylene oxide) (peo) blends. the in vitro antibacterial activity of cnfs was evaluated and the susceptibility/resistance of the selected bacteria toward ...201728383516
an improved method for identification of small non-coding rnas in bacteria using support vector machine.bacterial small non-coding rnas (srnas) are not translated into proteins, but act as functional rnas. they are involved in diverse biological processes like virulence, stress response and quorum sensing. several high-throughput techniques have enabled identification of srnas in bacteria, but experimental detection remains a challenge and grossly incomplete for most species. thus, there is a need to develop computational tools to predict bacterial srnas. here, we propose a computational method to ...201728383059
molecular mechanism of mrna repression in trans by a proq-dependent small rna.research into post-transcriptional control of mrnas by small noncoding rnas (srnas) in the model bacteria escherichia coli and salmonella enterica has mainly focused on srnas that associate with the rna chaperone hfq. however, the recent discovery of the protein proq as a common binding partner that stabilizes a distinct large class of structured srnas suggests that additional rna regulons exist in these organisms. the cellular functions and molecular mechanisms of these new proq-dependent srnas ...201728336682
ag/ago nanoparticles grown via time dependent double mechanism in a 2d layered ni-pcp and their antibacterial efficacy.a simple synthesis route for growth of ag/ago nanoparticles (nps) in large quantitative yields with narrow size distribution from a functional, non-activated, ni (ii) based highly flexible porous coordination polymer (pcp) as a template has been demonstrated. this template is a stable storage media for the nps larger than the pore diameters of the pcp. from epr study it was concluded that nps were synthesized via two mechanisms i.e. acid formation and the redox activity of the framework. size ra ...201728322256
potent immune responses induced by a salmonella ghost delivery system that expresses the recombinant stx2eb, fedf, and feda proteins of the escherichia coli-producing f18 and shiga toxin in a murine model and evaluation of its protective effect as a porcine vaccine candidate.in the pathogenicity of porcine edema disease, which is caused by the escherichia coli-producing f18 and shiga toxin, f18(+) fimbrial adhesins and shiga toxin 2e (stx2e) play pivotal roles in the colonization and enterotoxicity of this pathogen.201728317440
power and time dependent microwave assisted fabrication of silver nanoparticles decorated cotton (sndc) fibers for bacterial decontamination.plasmonic nanoparticles (nps) such as silver and gold have fascinating optical properties due to their enhanced optical sensitivity at a wavelength corresponding to their surface plasmon resonance (spr) absorption. present work deals with the fabrication of silver nanoparticles decorated cotton (sndc) fibers as a cheap and efficient point of contact disinfectant. sndc fibers were fabricated by a simple microwave assisted route. the microwave power and irradiation time were controlled to optimize ...201728316594
inducible spy transcription acts as a sensor for envelope stress of salmonella typhimurium.salmonella enterica infects a broad range of host animals, and zoonostic infection threatens both public health and the livestock and meat processing industries. many antimicrobials have been developed to target salmonella envelope that performs essential bacterial functions; however, there are very few analytical methods that can be used to validate the efficacy of these antimicrobials. in this study, to develop a potential biosensor for salmonella envelope stress, we examined the transcription ...201728316480
encapsulated whey-native yeast kluyveromyces marxianus as a feed additive for animal production.whey is the main byproduct of the cheese industry. while the composition is variable, it retains up to 55% of milk nutrients. the beneficial features of whey indicates a promising source of new potentially probiotic strains for the development of food additives destined for animal production. the aim of this study was to identify kluyveromyces spp. isolated from whey, to study some probiotic properties and to select the best strain to be encapsulated using derivatised chitosan. kluyveromyces mar ...201728277172
enabling complex genetic circuits to respond to extrinsic environmental signals.genetic circuits have the potential to improve a broad range of metabolic engineering processes and address a variety of medical and environmental challenges. however, in order to engineer genetic circuits that can meet the needs of these real-world applications, genetic sensors that respond to relevant extrinsic and intrinsic signals must be implemented in complex genetic circuits. in this work, we construct the first and and nand gates that respond to temperature and ph, two signals that have ...201728262949
ecotoxicological survey of mnei and y65r-mnei proteins as new potential high-intensity sweeteners.low-calorie sweeteners are widespread. they are routinely introduced into commonly consumed food such as diet sodas, cereals, and sugar-free desserts. recent data revealed the presence in considerable quantities of some of these artificial sweeteners in water samples qualifying them as a class of potential new emerging contaminants. this study aimed at evaluating the ecotoxicity profile of mnei and y65r-mnei, two engineered products derived from the natural protein monellin, employing representa ...201728251536
a novel salmonella strain inactivated by a regulated autolysis system and expressing the b subunit of shiga toxin 2e efficiently elicits immune responses and confers protection against virulent stx2e-producing escherichia coli.salmonella typhimurium (s. typhimurium) inactivated by a regulated autolysis system was genetically engineered to express the homo-pentameric b subunit of shiga toxin 2e (stx2eb) on its surface. to prepare a strain able to yield autolyzed salmonella bearing stx2eb, the plasmid pjhl184 harboring stx 2eb gene was transformed into the attenuated s. typhimurium strain, jol1454. stx2eb subcloned into the antigen delivery cassette of the plasmid was expressed as fusion protein with the outer membrane ...201728143511
the mechanism of killing by the proline-rich peptide bac7(1-35) against clinical strains of pseudomonas aeruginosa differs from that against other gram-negative bacteria.pseudomonas aeruginosa infections represent a serious threat to worldwide health. proline-rich antimicrobial peptides (pr-amps), a particular group of peptide antibiotics, have demonstrated in vitro activity against p. aeruginosa strains. here we show that the mammalian pr-amp bac7(1-35) is active against some multidrug-resistant cystic fibrosis isolates of p. aeruginosa by confocal microscopy and cytometric analyses, we investigated the mechanism of killing against p. aeruginosa strain pao1 and ...201728137800
bacterium-derived cell-penetrating peptides deliver gentamicin to kill intracellular pathogens.commonly used antimicrobials show poor cellular uptake and often have limited access to intracellular targets, resulting in low antimicrobial activity against intracellular pathogens. an efficient delivery system to transport these drugs to the intracellular site of action is needed. cell-penetrating peptides (cpps) mediate the internalization of biologically active molecules into the cytoplasm. here, we characterized two cpps, α1h and α2h, derived from the yersinia enterocolitica yopm effector ...201728096156
anti-folates potentiate bactericidal effects of other antimicrobial agents.synergies between sulfonamides and other antimicrobial agents have long been reported, but the reason still remains unclear. previously, vilchèze et al. found that, sulfamethoxazole (smx) could potentiate the bacterialcidal activity of isoniazid (inh) and rifampin (rif) in mycobacterium tuberculosis. to test if this was also the case in other bacteria, the ability to potentiate bactericidal effect of rif by smx was evaluated in escherichia coli, staphylococcus aureus, salmonella typhimurium and ...201728074051
surface layer protein from lactobacillus acidophilus ncfm inhibit intestinal pathogen-induced apoptosis in ht-29 cells.intestinal pathogens have been proposed to adhere to epithelial cells and cause apoptosis. this study was to investigate the inhibitory effects of surface layer protein (slp, 46kda) from lactobacillus acidophilus ncfm on escherichia coli and salmonella-induced apoptosis in ht-29 cells and the mechanism of the inhibition was also studied. the slp could alleviate the chromatin condensation caused by intestinal pathogens as observed under fluorescent microscope. flow cytometry analysis showed that ...201728057572
the fate of salmonella typhimurium and escherichia coli o157 on hot boned versus conventionally chilled beef.this study investigated the fate of salmonella typhimurium and escherichia coli o157 on hot boned versus conventionally chilled beef. beef samples were individually inoculated with s. typhimurium atcc 14028, s. typhimurium 844, e. coli o157 edl 933 or e. coli t13. half the samples were subject to the same time-temperature chilling profile used for conventionally chilling beef carcasses while the other half was subject to hot boned conditions. the surface ph (5.5) and aw (0.95 to 0.97) were stabl ...201728038402
hydrophobic ionic liquids for quantitative bacterial cell lysis with subsequent dna quantification.dna is one of the most frequently analyzed molecules in the life sciences. in this article we describe a simple and fast protocol for quantitative dna isolation from bacteria based on hydrophobic ionic liquid supported cell lysis at elevated temperatures (120-150 °c) for subsequent pcr-based analysis. from a set of five hydrophobic ionic liquids, 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide was identified as the most suitable for quantitative cell lysis and dna extraction beca ...201728004172
subinhibitory concentrations of disinfectants promote the horizontal transfer of multidrug resistance genes within and across genera.the greater abundances of antibiotic resistance genes (args) in point-of-use tap and reclaimed water than that in freshly treated water raise the question whether residual disinfectants in distribution systems facilitate the spread of args. this study investigated three widely used disinfectants (free chlorine, chloramine, and hydrogen peroxide) on promoting args transfer within escherichia coli strains and across genera from escherichia coli to salmonella typhimurium. the results demonstrated t ...201727997135
evaluation of the performance of the iq-check kits and the usda microbiology laboratory guidebook methods for detection of shiga toxin-producing escherichia coli (stec) and stec and salmonella simultaneously in ground beef.to evaluate the performance of the iq-check kits and the usda microbiology laboratory guidebook (mlg) methods for detection of the top seven shiga toxin-producing escherichia coli (stec) (o157:h7, o26, o45, o103, o111, o121 and o145) in ground beef and both stec and salmonella in co-inoculated samples.201727987365
regulation of nrf operon expression in pathogenic enteric bacteria: sequence divergence reveals new regulatory complexity.the escherichia coli k-12 nrf operon encodes a periplasmic nitrite reductase, the expression of which is driven from a single promoter, pnrf. expression from pnrf is activated by the fnr transcription factor in response to anaerobiosis and further increased in response to nitrite by the response regulator proteins, narl and narp. fnr-dependent transcription is suppressed by the binding of two nucleoid associated proteins, ihf and fis. as fis levels increase in cells grown in rich medium, the pos ...201728211111
attachment of 13 types of foodborne bacteria to jalapeño and serrano peppers and antibacterial effect of roselle calyx extracts, sodium hypochlorite, colloidal silver, and acetic acid against these foodborne bacteria on peppers.chili peppers are a very important crop in mexico. however, these peppers have been associated with salmonella infection outbreaks in the united states, and salmonella and diarrheagenic escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in mexico. to decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. roselle ( h ...201728199144
prevalence and molecular characterization of mcr-1-positive salmonella strains recovered from clinical specimens in china.the recently discovered colistin resistance-encoding element, mcr-1, adds to the list of antimicrobial resistance genes that rapidly erodes the antimicrobial efficacy of not only the commonly used antibiotics, but also the last line agents of carbapenems and colistin. this study investigated the prevalence of the mobile colistin resistance determinant mcr-1 in salmonella strains recovered from clinical settings in china, and the transmission potential of mcr-1-bearing mobile elements harbored by ...201728193662
self-oligomerizing structure of the flagellar cap protein flid and its implication in filament assembly.flid is a self-oligomerizing structural protein that caps the growing end of the bacterial flagellar filament. flid also plays a key role in the flagellar system by continuously adding a new flagellin protein to the tip of the filament. to structurally characterize flid oligomerization and to provide a flid-mediated flagellin polymerization mechanism, we have determined the crystal structures of flid proteins from escherichia coli and salmonella enterica serovar typhimurium (ecflid and stflid, r ...201728179186
ca(2+)-dependent focal exocytosis of golgi-derived vesicles helps phagocytic uptake in macrophages.the role of golgi apparatus during phagocytic uptake by macrophages has been ruled out in the past. notably, all such reports were limited to fcγ receptor-mediated phagocytosis. here, we unravel a highly devolved mechanism for recruitment of golgi-derived secretory vesicles during phagosome biogenesis, which was important for uptake of most cargos, except the igg-coated ones. we report recruitment of mannosidase-ii-positive golgi-derived vesicles during uptake of diverse targets, including latex ...201728174296
the promiscuous suma missense suppressor from salmonella enterica has an intriguing mechanism of action.while most missense suppressors have very narrow specificities and only suppress the allele against which they were isolated, the suma missense suppressor from salmonella enterica serovar typhimurium is a promiscuous or broad-acting missense suppressor that suppresses numerous missense mutants. the suma missense suppressor was identified as a glyv trna gly3(gau/c) missense suppressor that can recognize gau or gac aspartic acid codons and insert a glycine amino acid instead of aspartic acid. in a ...201727974497
contribution of asparagine catabolism to salmonella virulence.salmonellae are pathogenic bacteria that cause significant morbidity and mortality in humans worldwide. salmonellae establish infection and avoid clearance by the immune system by mechanisms that are not well understood. we previously showed that l-asparaginase ii produced by salmonella enterica serovar typhimurium (s typhimurium) inhibits t cell responses and mediates virulence. in addition, we previously showed that asparagine deprivation such as that mediated by l-asparaginase ii of s typhimu ...201727849183
comparative evaluation of 11 in silico models for the prediction of small molecule mutagenicity: role of steric hindrance and electron-withdrawing groups.the goal of this investigation was to perform a comparative analysis on how accurately 11 routinely-used in silico programs correctly predicted the mutagenicity of test compounds that contained either bulky or electron-withdrawing substituents. to our knowledge this is the first study of its kind in the literature. such substituents are common in many pharmaceutical agents so there is a significant need for reliable in silico programs to predict precisely whether they truly pose a risk for mutag ...201727813437
an integrated study on antimicrobial activity and ecotoxicity of quantum dots and quantum dots coated with the antimicrobial peptide indolicidin.this study attempts to evaluate the antimicrobial activity and the ecotoxicity of quantum dots (qds) alone and coated with indolicidin. to meet this objective, we tested the level of antimicrobial activity on gram-positive and gram-negative bacteria, and we designed an ecotoxicological battery of test systems and indicators able to detect different effects using a variety of end points. the antibacterial activity was analyzed against staphylococcus aureus (atcc 6538), pseudomonas aeruginosa (atc ...201727616887
genotoxicity evaluation of hwanglyeonhaedok-tang, an herbal formula.hwanglyeonhaedok-tang (huanglianjiedu-tang, orengedoku-to), a traditional herbal formula, is used for the treatment of inflammatory, gastrointestinal and cardiovascular diseases.201727916588
short chain nitrocompounds as a treatment of layer hen manure and litter; effects on in vitro survivability of salmonella, generic e. coli and nitrogen metabolism.the current study was conducted to assess the bactericidal effectiveness of several nitrocompounds against pathogens in layer hen manure and litter. evidence from an initial study indicated that treatment of layer hen manure with 12 mm nitroethane decreased populations of generic e. coli and total coliforms by 0.7 and 2.2 log10 colony forming units (cfu) g(-1), respectively, after 24 h aerobic incubation at ambient temperature when compared to untreated populations. salmonella concentrations wer ...201727628961
in vitro safety assessments and antimicrobial activities of lactobacillus rhamnosus strains isolated from a fermented mare's milk.safety and probiotic characteristics such as antimicrobial activities of three lactobacillus rhamnosus strains, fsmm15, fsmm22 and fsmm26, previously isolated as potential probiotics from fermented mare's milk were investigated. the three fsmm strains were susceptible to ampicillin, gentamycin, kanamycin, streptomycin, tetracycline and chloramphenicol, whereas they were resistant to erythromycin (minimal inhibitory concentration (mic) = 4-8 µg/ml) and clindamycin (mic = 4 µg/ml); bioconversion o ...201727476815
isolation and identification of a new saponin from cephalaria aytachii.a new hederagenin-type triterpenoid glycoside (1) named aytachoside a, along with eight known triterpene glycosides, were isolated from the aerial parts of cephalaria aytachii gokturk and sumbul (dipsacaceae). the structures of compounds 1-9 were determined by spectroscopic (1d and 2d nmr, hresims) and chemical examinations. the antimicrobial effect of compound 1 was found considerably active against escherichia coli, pseudomonas aeruginosa and especially salmonella typhimurium microorganisms us ...201727454791
effect of iranian ziziphus honey on growth of some foodborne pathogens.honey has previously been shown to have wound healing and antimicrobial properties, but this is dependent on the type of honey, geographical location, and flower from which the final product is derived. we tested the antimicrobial activity of a natural honey originating from the ziziphus spina-christi tree, against selected strains of bacteria. ziziphus honey among more than a 100 verities of honey is known to have the greatest value of energy and minerals in it.201727003970
biofilm-associated bacterial amyloids dampen inflammation in the gut: oral treatment with curli fibres reduces the severity of hapten-induced colitis in mice.a disruption of epithelial barrier function can lead to intestinal inflammation. toll-like receptor (tlr) 2 activation by microbial products promotes intestinal epithelial integrity and overall gut health. several bacterial species, including enteric bacteria, actively produce amyloid proteins as a part of their biofilms. recognition of amyloid fibres found in enteric biofilms, termed curli, by the toll-like receptor (tlr)2/1 complex reinforces barrier function. here, we investigated the effect ...201726855788
orally administered live attenuated salmonella typhimurium protects mice against lethal infection with h1n1 influenza virus.pre-stimulation of toll-like receptors (tlrs) by agonists has been shown to increase protection against influenza virus infection. in this study, we evaluated the protective response generated against influenza a/puerto rico/8/1934 (pr8; h1n1) virus by oral and nasal administration of live attenuated salmonella enterica serovar typhimurium, jol911 strain, in mice. oral and nasal inoculation of jol911 significantly increased the mrna copy number of tlr-2, tlr4 and tlr5, and downstream type i inte ...201728284594
modeling the potential for vaccination to diminish the burden of invasive non-typhoidal salmonella disease in young children in mali, west africa.in sub-saharan africa, systematic surveillance of young children with suspected invasive bacterial disease (e.g., septicemia, meningitis) has revealed non-typhoidal salmonella (nts) to be a major pathogen exhibiting high case fatality (~20%). where infant vaccination against haemophilus influenzae type b (hib) and streptococcus pneumoniae has been introduced to prevent invasive disease caused by these pathogens, as in bamako, mali, their burden has decreased markedly. in parallel, nts has become ...201728182657
rampant infections of bone marrow stem cell niches as triggers for spondyloarthropathies and rheumatoid arthritis.tropheryma whipplei can induce rheumatism mimicking spa or ra, but even more rampant bacterial/viral infections in epiphyseal bones could also contribute to the onset of ra and spa. indeed, as bone marrow stem cell niches are enriched in tregs and myeloid derived suppressor cells, these areas are favourable for the persistence of quiescent viruses and/or dormant bacteria. this review focuses on the possibility that such silent infections of bone marrow stem cell niches might contribute to the pa ...201726886813
a systematic review of the incidence, risk factors and case fatality rates of invasive nontyphoidal salmonella (ints) disease in africa (1966 to 2014).this study systematically reviews the literature on the occurrence, incidence and case fatality rate (cfr) of invasive nontyphoidal salmonella (ints) disease in africa from 1966 to 2014. data on the burden of ints disease in africa are sparse and generally have not been aggregated, making it difficult to describe the epidemiology that is needed to inform the development and implementation of effective prevention and control policies. this study involved a comprehensive search of pubmed and embas ...201728056035
identification of invasive salmonella enterica serovar typhimurium st313 in ambulatory hiv-infected adults in mozambique.despite evidence describing the burden of invasive non-typhoidal salmonella (ints) disease in sub-saharan africa, ints is not recognized as a priority within global health policy institutions. recently, salmonella enterica serovar typhimurium, sequence type (st) 313, has been identified as the predominant cause of ints disease in multiple sub-saharan african countries.201726751031
mcr-1.3: a new mcr variant carried by an incp plasmid in a colistin-resistant salmonella enterica serovar typhimurium isolated from a healthy individual.in this study, we reported a novel mcr-1 gene variant, named mcr-1.3, carried by an incp plasmid in a colistin-resistant salmonella typhimurium from a healthy person. compared with mcr-1, the mcr-1.3 gene contained two snps; one of them resulted in an arginine to histidine variation (arg536->his). the plasmid carrying mcr-1.3 gene was designated pmcr1.3_p053, and was highly similar to a recently discovered mcr-1-bearing plasmid found in klebsiella pneumoniae.201728264851
potential anti-inflammatory, antioxidant and antimicrobial activities of sambucus australis.sambucus australis cham. & schltdl. (adoxaceae) is used in brazilian folk medicine to treat inflammatory disorders.201728166708
expression and characterization of recombinant chicken mannose binding lectin.mannose binding lectin (mbl) is a serum collagenous c-type lectin that plays an important role in the innate immune protection against pathogens. previously, human and mouse studies have demonstrated that mbl binds a broad range of pathogens that results in their neutralization through agglutination, enhanced phagocytosis, and/or complement activation via the lectin pathway. the role of mbl in chicken is not well understood although the mbl concentration in serum seems to correlate with protecti ...201727817988
anti-salmonella activity and probiotic trends of kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 isolated from a french cheese, tomme d'orchies.kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 were recently isolated from a traditional french cheese, tomme d'orchies, and characterized here for their advantages using a different application perspective. first, we established their anti-salmonella activity and downregulation of the virulence sopd gene of salmonella enterica subsp. enterica serovar typhimurium, mainly in the presence of k. marxianus s-2-05. in addition to their antagonism, these non-saccharomyces yeasts were a ...201728366839
probiotic potential of a lactobacillus bacterium of canine faecal-origin and its impact on select gut health indices and immune response of dogs.the objective of the present study was to develop a probiotic of canine-origin for its potential application in pet nutrition. accordingly, 32 lactic acid bacteria (lab) strains were isolated from faeces of dogs, out of which 9 strains were short-listed for further in vitro testing based on the aggregation time and cell surface hydrophobicity. the results of acid-, bile- and phenol-tolerance tests indicated that out of the nine, isolate cpro23 was having better resistance to these adverse condit ...201728188477
lactobacillus paracasei metabolism of rice bran reveals metabolome associated with salmonella typhimurium growth reduction.this study aimed to determine the effect of a cell-free supernatant of lactobacillus paracasei atcc27092 with and without rice bran extract (rbe) on salmonella typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions.201728371001
selection of lactic acid bacteria with probiotic potential isolated from the fermentation process of "cupuaçu" (theobroma grandiflorum).in the present study, nine lactic acid bacteria isolated from the fermentation process of "cupuaçu" (theobroma grandiflorum) were selected for probiotic use. in vitro (resistance to gastrointestinal environment, in vitro antagonism and co-aggregation with pathogens) and in vivo (intestinal colonization and ex vivo antagonism in germ-free mice, cumulative mortality, translocation to liver and spleen, histopathological examination of liver and ileum and mrna cytokine gene expression during an expe ...201728224483
effect of lactobacillus plantarum tennozu-su2 on salmonella typhimurium infection in human enterocyte-like ht-29-luc cells and balb/c mice.the probiotic properties and inhibitory effect on salmonella typhimurium adhesion on human enterocyte-like ht-29-luc cells of three lactobacillus plantarum strains isolated from fermented fish, beach sand and a coastal plant were determined. compared with the type strain l. plantarum nbrc 15891(t), which was isolated from pickled cabbage, l. plantarum tennozu-su2 isolated from the acorn of a coastal tree showed high autoaggregation in de man, rogosa and sharpe (mrs) broth and an antagonistic eff ...201727943051
cold plasma-activated hydrogen peroxide aerosol inactivates escherichia coli o157:h7, salmonella typhimurium, and listeria innocua and maintains quality of grape tomato, spinach and cantaloupe.the purpose of this study was to investigate the efficacy of aerosolized hydrogen peroxide in inactivating bacteria and maintaining quality of grape tomatoes, baby spinach leaves and cantaloupes. stem scars and smooth surfaces of tomatoes, spinach leaves, and cantaloupe rinds, inoculated with escherichia coli o157:h7, salmonella typhimurium and listeria innocua, were treated for 45s followed by additional 30min dwell time with hydrogen peroxide (7.8%) aerosols activated by atmospheric cold plasm ...201728319798
comparative effects of untreated and 3-methacryloxypropyltrimethoxysilane treated zno nanoparticle reinforcement on properties of polylactide-based nanocomposite films.polylactide (pla) nanocomposites characterized by antimicrobial properties are gaining increasing attention for food packaging. in this contribution, the pla based nanocomposite films with multifunctional end-use properties were achieved by incorporating zno nanoparticles (nps) [untreated: zno(ut) and 3-methacryloxypropyltrimethoxysilane treated: zno(st)] into polymer matrix via solvent casting method. the zno(st) prevented the degradation of pla at higher temperature and improved the mechanical ...201728366847
teriyaki sauce with carvacrol or thymol effectively controls escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and indigenous flora in marinated beef and marinade.an effective bactericidal cold-marinating method for beef products is described, exploiting the synergism between soy sauce and natural compounds (carvacrol, cv or thymol, tm) to reduce microbiological risks. beef slices inoculated with escherichia coli o157:h7, listeria monocytogenes, and salmonella typhimurium (3.1-3.5logcfu/g) were marinated in a teriyaki sauce with or without cv and tm (0.3 and 0.5%). after 1, 3, and 7days at 4°c, indigenous microflora population, color, lipid oxidation, mar ...201728285115
inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in ready-to-bake cookie dough by gamma and electron beam irradiation.this study was conducted to investigate the efficacy of gamma and electron beam irradiation to inactivate foodborne pathogens in ready-to-bake cookie dough and to determine the effect on quality by measuring color and texture changes. cookie dough inoculated with escherichia coli o157:h7, salmonella typhimurium, or listeria monocytogenes was subjected to gamma and electron beam irradiation, with doses ranging from 0 to 3 kgy. as the radiation dose increased, the inactivation effect increased amo ...201728213023
pathogen biofilm formation on cantaloupe surface and its impact on the antibacterial effect of lauroyl arginate ethyl.pathogen biofilm at fruit surface may pose a particular risk to food safety. in this study, the biofilms of listeria monocytogenes v7 and salmonella enterica serovar typhimurium atcc 13311 on cantaloupe fruit surface were visualized, and the resistance of biofilms against lauroyl arginate ethyl (lae, an antibacterial compound) was evaluated. each bacterium was inoculated on isolated cantaloupe rind surfaces at 10(5)-10(6) cfu/cm(2) and after incubation for 2, 12, 24, and 48 h, the surfaces were ...201728213018
stevia-based sweeteners as a promising alternative to table sugar: the effect on listeria monocytogenes and salmonella typhimurium growth dynamics.sugar is commonly substituted with stevia-based products in food industry and in our daily-life. this substitution results in a change in food product characteristic formula and properties that may affect the growth dynamics of food pathogenic and spoilage bacteria. this work studies the effect of table sugar (ts), laboratory sucrose (ls), commercial stevia (st) and steviol glycosides (sg) on the growth dynamics of salmonella typhimurium and listeria monocytogenes. experiments were carried out i ...201728126702
functional constituents of wild and cultivated goji (l. barbarum l.) leaves: phytochemical characterization, biological profile, and computational studies.goji (lycium barbarum l.) leaves are emphasized as a functional tea or as dietary supplements. the phenolic compound profile, antioxidant, enzyme inhibitory, antimicrobial, and antimutagenic activities of leaf extracts from two selected cultivars in comparison with wild-growing plants have been evaluated. hplc-dad/esi-tof-ms analysis revealed the presence of phenolic acids and flavonoids with chlorogenic acid and rutin being the dominant compounds in the cultivated plants, whereas rutin and kaem ...201728095717
promyelocytic leukemia protein (pml) controls listeria monocytogenes infection.the promyelocytic leukemia protein (pml) is the main organizer of stress-responsive subnuclear structures called pml nuclear bodies. these structures recruit multiple interactors and modulate their abundance or their posttranslational modifications, notably by the sumo ubiquitin-like modifiers. the involvement of pml in antiviral responses is well established. in contrast, the role of pml in bacterial infection remains poorly characterized. here, we show that pml restricts infection by the patho ...201728074026
application of low frequency pulsed ohmic heating for inactivation of foodborne pathogens and ms-2 phage in buffered peptone water and tomato juice.the purpose of this study was to inactivate foodborne pathogens effectively by ohmic heating in buffered peptone water and tomato juice without causing electrode corrosion and quality degradation. escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes were used as representative foodborne pathogens and ms-2 phage was used as a norovirus surrogate. buffered peptone water and tomato juice inoculated with pathogens were treated with pulsed ohmic heating at different frequencie ...201728040173
application of a 222-nm krypton-chlorine excilamp to control foodborne pathogens on sliced cheese surfaces and characterization of the bactericidal mechanisms.this study was conducted to investigate the basic spectral properties of a 222-nm krypton-chlorine (krcl) excilamp and its inactivation efficacy against major foodborne pathogens on solid media, as well as on sliced cheese compared to a conventional 254-nm low-pressure mercury (lp hg) lamp. selective media and sliced cheese inoculated with escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes were irradiated with a krcl excilamp and a lp hg lamp at the sam ...201728039742
surface-enhanced raman spectroscopy introduced into the international standard organization (iso) regulations as an alternative method for detection and identification of pathogens in the food industry.we show that surface-enhanced raman spectroscopy (sers) coupled with principal component analysis (pca) can serve as a fast, reliable, and easy method for detection and identification of food-borne bacteria, namely salmonella spp., listeria monocytogenes, and cronobacter spp., in different types of food matrices (salmon, eggs, powdered infant formula milk, mixed herbs, respectively). the main aim of this work was to introduce the sers technique into three iso (6579:2002; 11290-1:1996/a1:2004; 22 ...201728004171
impact of food model (micro)structure on the microbial inactivation efficacy of cold atmospheric plasma.the large potential of cold atmospheric plasma (cap) for food decontamination has recently been recognized. room-temperature gas plasmas can decontaminate foods without causing undesired changes. this innovative technology is a promising alternative for treating fresh produce. however, more fundamental studies are needed before its application in the food industry. the impact of the food structure on cap decontamination efficacy of salmonella typhimurium and listeria monocytogenes was studied. c ...201727507138
impact of starch-based emulsions on the antibacterial efficacies of nisin and thymol in cantaloupe juice.the use of antimicrobial compounds to prevent foodborne pathogens from contaminating fresh-cut produce has received broad attentions; however, the applications of these compounds are hindered by their rapid depletion in foods. to prolong their efficacies, the use of delivery systems is essential. in this study, oil-in-water emulsions formed using starch octenyl succinate (starch-os) were used to stabilize nisin and thymol in cantaloupe juice-containing fluid. listeria monocytogenes v7 and salmon ...201727664621
alpk1 controls tifa/traf6-dependent innate immunity against heptose-1,7-bisphosphate of gram-negative bacteria.during infection by invasive bacteria, epithelial cells contribute to innate immunity via the local secretion of inflammatory cytokines. these are directly produced by infected cells or by uninfected bystanders via connexin-dependent cell-cell communication. however, the cellular pathways underlying this process remain largely unknown. here we perform a genome-wide rna interference screen and identify tifa and traf6 as central players of shigella flexneri and salmonella typhimurium-induced inter ...201728222186
novel aldo-keto reductases for the biocatalytic conversion of 3-hydroxybutanal to 1,3-butanediol: structural and biochemical studies.the nonnatural alcohol 1,3-butanediol (1,3-bdo) is a valuable building block for the synthesis of various polymers. one of the potential pathways for the biosynthesis of 1,3-bdo includes the biotransformation of acetaldehyde to 1,3-bdo via 3-hydroxybutanal (3-hb) using aldolases and aldo-keto reductases (akrs). this pathway requires an akr selective for 3-hb, but inactive toward acetaldehyde, so it can be used for one-pot synthesis. in this work, we screened more than 20 purified uncharacterized ...201728130301
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