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comparative gene expression profiling of milk somatic cells of sahiwal cattle and murrah buffaloes.india is the world's largest milk producing country because of massive contribution made by cattle and buffaloes. in the present investigation, comprehensive comparative profiling of transcriptomic landscape of milk somatic cells of sahiwal cattle and murrah buffaloes was carried out. genes with highest transcript abundance in both species were enriched for biological processes such as lactation, immune response, cellular oxidant detoxification and response to hormones. analysis of differential ...202132877747
structural analysis of covid-19 spike protein in recognizing the ace2 receptor of different mammalian species and its susceptibility to viral infection.the pandemic covid-19 was caused by a novel coronavirus-2 (sars-cov-2) that infects humans through the binding of glycosylated sars-cov-2 spike 2 protein to the glycosylated ace2 receptor. the spike 2 protein recognizes the n-terminal helices of the glycosylated metalloprotease domain in the human ace2 receptor. to understand the susceptibility of animals for infection and transmission, we did sequence and structure-based molecular interaction analysis of 16 ace2 receptors from different mammali ...202133552834
viral infections shared between water buffaloes and small ruminants in switzerland.importation of exotic animals that may harbor infectious agents poses risks for native species with potentially severe impacts on animal health and animal production. although the asian water buffalo (bubalus bubalis) population in europe is steadily increasing, its susceptibility to viral infections and its role for interspecies transmission is largely unknown. to identify viral infections that are shared between exotic water buffaloes and native small ruminants, we collected blood samples from ...202134166139
evaluation of safety and efficacy of an inactivated marker vaccine against bovine alphaherpesvirus 1 (bohv-1) in water buffalo (bubalus bubalis).recent studies have explored the seropositivity of bovine alphaherpesvirus 1 (bohv-1) in water buffaloes, suggesting the urgency for developing strategies to eradicate the virus involving both cattle and water buffaloes. however, in europe, the glycoprotein e (ge) deleted marker vaccines against bohv-1 are commercially available only for the cattle industry. this study, for the first time, evaluated the safety and efficacy of a commercial inactivated ge-deleted marker vaccine in water buffalo. f ...202133917160
characterization of foot and mouth disease virus serotype sat-2 in swamp water buffaloes (bubalus bubalis) under the egyptian smallholder production system.spontaneous mutations are a common characteristic of the foot and mouth disease virus (fmdv), leading to wide antigenic variations resulting in the emergence of new topotypes and lineages of fmdv, which contributes to occasional vaccination failures. the objectives of the present study were to genetically characterize fmdv isolated from water buffaloes and study the biochemical and histopathological indicators of infected animals. fifty-four water buffaloes of both sexes and different ages suffe ...202134200281
genomic analyses reveal distinct genetic architectures and selective pressures in buffaloes.the domestic buffalo (bubalus bubalis) is an essential farm animal in tropical and subtropical regions, whose genomic diversity is yet to be fully discovered.202032083286
glutathione-s-transferase: an important diagnostic antigen of liver amphistome gigantocotyle explanatum, infecting the indian water buffalo.the foodborne trematodiases pose a significant health problem to the animals as well as the human population living in close proximities with the livestock and are still considered as the neglected tropical diseases by the world health organisation. the digenetic trematode, gigantocotyle explanatum infecting the liver of indian water buffalo, bubalus bubalis, has been identified as one of the most common helminth parasite responsible for the disease, amphistomosis, in livestock. despite huge aba ...202032081660
genomic measures of inbreeding coefficients and genome-wide scan for runs of homozygosity islands in iranian river buffalo, bubalus bubalis.consecutive homozygous fragments of a genome inherited by offspring from a common ancestor are known as runs of homozygosity (roh). roh can be used to calculate genomic inbreeding and to identify genomic regions that are potentially under historical selection pressure. the dataset of our study consisted of 254 azeri (az) and 115 khuzestani (khz) river buffalo genotyped for ~ 65,000 snps for the following two purposes: 1) to estimate and compare inbreeding calculated using roh (froh), excess of h ...202032041535
detection of polymorphism within leptin gene in egyptian river buffalo and predict its effects on different molecular levels.leptin (lep) regulates the glucose homeostasis directly and centrally by the regulation of the insulin levels or indirectly by alternation of the levels of the other glucose metabolism regulator hormones. the present investigation studied the polymorphism in lep gene which is related to fertility in 81 female egyptian river buffalo.202032037476
investigating genetic variability in hsp70 gene-5'-fragment and its association with thermotolerance in murrah buffalo (bubalus bubalis) under sub-tropical climate of india.the present study was undertaken to investigate genetic variability in a fragment comprising 5'utr along with partial coding sequence of hsp70 gene and its association with thermotolerance traits in murrah buffalo at icar-research complex for eastern region, patna (india). the allelic variants were identified from genomic dna samples using sscp technique. the pcr products were sequenced and analyzed. data on different thermotolerance traits recorded in three seasons were analyzed by least square ...202032020511
embryo transfer in buffalo (bubalus bubalis).the use of assisted reproductive technologies, such as superovulation and in vivo embryo production and in vitro embryo production (ivep), has increased rapidly in recent years and is now applied worldwide for genetic improvement in beef and dairy buffaloes. although in vivo embryo production has been shown to be feasible in buffalo, low efficiency and limited commercial application has been documented. these results could be associated with low antral follicle populations, high levels of follic ...202031996292
asian water buffalo: domestication, history and genetics.the domestic asian water buffalo (bubalus bubalis) is found on all five continents, with a global population of some 202 million. the livelihoods of more people depend on this species than on any other domestic animal. the two distinct types (river and swamp) descended from different wild asian water buffalo (bubalus arnee) populations that diverged some 900 kyr bp and then evolved in separate geographical regions. after domestication in the western region of the indian subcontinent (ca. 6300 ye ...202031967365
effects of treatment with a microrna mimic or inhibitor on the developmental competence, quality, epigenetic status and gene expression of buffalo (bubalus bubalis) somatic cell nuclear transfer embryos.expression levels of 13 micrornas (mirnas) were compared between buffalo blastocysts produced by somatic cell nuclear transfer through hand-made cloning and ivf to improve cloning efficiency. expression of mir-22, mir-145, mir-374a and mir-30c was higher, whereas that of mir-29b, mir-101, mir-302b, mir-34a, mir-21 and mir-25 was lower, in nuclear transferred (nt) than ivf embryos; the expression of mir-200b, mir-26a and mir-128 was similar between the two groups. based on these, mir-145, which i ...202031959280
local peritonitis and omental bursitis as sequelae of abomasal ulceration in buffaloes (bubalus bubalis): clinical findings and outcome of treatment.abomasal ulcers are difficult to diagnose clinically with limited therapeutic approach to combat the disease. omental bursitis (ob) and local peritonitis (lp) are known sequelae of abomasal ulcer in cows. in this study, differentiation between ob and lp in regard to clinical symptoms, biochemical analyses and the response to treatment in buffaloes was done. twenty buffaloes (bubalus bubalis) were admitted with a history of intermittent appetite, wasting and mild abdominal distension during the p ...202031955661
relationship between angioarchitecture of the testicular artery and spermiogram parameters in egyptian buffalo bulls (bubalus bubalis).the current study aimed to investigate the effect of testicular artery angioarchitecture on the spermiogram parameters in egyptian buffalo bulls. eight adult buffalo bulls aged between 2 and 8 years were used for semen evaluation. for anatomical studies, the masculine gonads were collected after slaughtering 30 adult bulls and prepared for injection by different masses (urographine® , latex and epoxy) through the testicular artery. the mass activity of the ejaculate was assessed immediately afte ...202031944422
effects of different culture systems on the culture of prepuberal buffalo (bubalus bubalis) spermatogonial stem cell-like cells in vitro.currently, the systems for culturing buffalo spermatogonial stem cells (sscs) in vitro are varied, and their effects are still inconclusive. in this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (knockout serum replacement) materials on the in vitro culture of buffalo ssc-like cells. significantly more ddx4- and uchl1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined m ...202031940692
normal anatomic reference of pastern and coffin joints in egyptian buffalo (bubalus bubalis): a compared atlas of cross-sectional anatomy, magnetic resonance imaging and computed tomography.the purpose of this study was to describe normal magnetic resonance imaging and computed tomographic anatomy of pastern and coffin joints in egyptian buffalo using cadaveric distal limbs. this study was achieved using twelve fresh cadaveric distal limbs from adult healthy buffaloes of both sexes. these cadaveric limbs were scanned using a 1 tesla mri scanner and ct scanner, injected with red latex, frozen at -20°c for 1 week, and then sectioned into sagittal, dorsal and transverse slices. the ob ...202031910304
effect of carboxylated poly l-lysine as a cryoprotectant on post-thaw quality and in vivo fertility of nili ravi buffalo (bubalus bubalis) bull semen.buffalo bull sperm are more prone to cryo-injuries. glycerol being the most common permeable cryoprotectant exerts cytotoxic effects on sperm which cause a reduction in fertility. thus, the exploration of new cryoprotectant is needed. for this purpose, we investigated the effect of carboxylated poly l-lysine (cpll) as cryoprotectant used with different concentrations of glycerol on post-thaw sperm motility, kinematics, plasma membrane integrity, mitochondrial membrane potential (mmp), lipid pero ...202031884337
expression and functional role of fibroblast growth factors (fgf) in placenta during different stages of pregnancy in water buffalo (bubalus bubalis).the present study documented the expression and functional role of fibroblast growth factors (fgfs) family and their receptors (fibroblast growth factor receptor, fgfrs) in placenta (cotyledon; cot, caruncle; car) during different stages of pregnancy in water buffalo. samples were collected from early pregnancy 1 (ep1); early pregnancy 2 (ep2); mid pregnancy (mp) and late pregnancy (lp) while diestrus stage of oestrus cycle (np) was taken as control. in addition, modulatory role of fgf2 on mrna ...202031864010
a new three-dimensional glass scaffold increases the in vitro maturation efficiency of buffalo (bubalus bubalis) oocyte via remodelling the extracellular matrix and cell connection of cumulus cells.at present, many three-dimensional (3d) culture systems have been reported, improving the oocyte quality of in vitro maturation (ivm), yet the mechanism still needs to be further explored. here we examined the effects of a new self-made 3d glass scaffold on buffalo oocyte maturation; meanwhile, the underlying mechanism on buffalo oocyte maturation was also detected. compared to the two-dimensional (2d) glass dish culture, results revealed that the 3d culture can improve the first polar body rate ...202031816136
expression and localization of cellular flice-like inhibitory protein (cflip), an anti-apoptotic factor, in corpora lutea during the estrous cycle and pregnancy in thai swamp buffalo <i>(bubalus bubalis)</i>.in female mammals, luteal cells rapidly proliferate and form corpora lutea (cls) after ovulation. the corpus luteum (cl) plays crucial roles in establishing and maintaining pregnancy. to gain further insights into the role of cellular flice-like inhibitory protein (cflip), an anti-apoptosis factor that is structurally similar to procaspase-8 but lacks proteolytic enzyme activity, we examined the expression in cls of thai swamp buffalos (bubalus bubalis) during the early, mid, and late stage of t ...202031801913
cross comparison of seminal plasma proteins from cattle and buffalo (bubalus bubalis).the objective of this study was to evaluate seminal plasma proteins from cattle and buffalo (bubalus bubalis), to identify differences between related species. sixteen buffaloes and 16 cattle between 30 and 60 months of age were used. semen collection was performed by electroejaculation, followed by macroscopic and microscopic subjective analyses. after analysis, the samples were centrifuged at 800 g for 10 min, and the supernatant (seminal plasma) was recentrifuged at 10,000 g for 30 min at 4°c ...202031733131
supplementation of green tea extract (gte) in extender improves structural and functional characteristics, total antioxidant capacity and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa.the aim of this study was to elucidate the beneficial effects of green tea extract (gte) in tris citric acid extender on post thaw structural and functional characteristics, dna fragmentation (%), total antioxidant capacity (tac, μm/l), lipid peroxidation (lpo, μm/ml) levels and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa. gte is a acknowledged natural antioxidant, act as a free radical scavenger and protects spermatozoa against oxidative stress. three mature and donor buffal ...202031732163
successful transplantation of transfected enriched buffalo (bubalus bubalis) spermatogonial stem cells to homologous recipients.genetic modification of spermatogonial stem cells (sscs) is an alternative method to pronuclear microinjection and somatic cell nuclear transfer for transgenesis in large animals. in the present study, we optimized the process of homologous ssc transplantation in the water buffalo (bubalus bubalis) using transfected enriched sscs generated by a non-viral transfection approach. firstly, the ssc enrichment efficiencies of extracellular matrix components viz. collagen, gelatin, and datura stramoniu ...202031711692
evaluation of a bovine cathelicidin elisa for detecting mastitis in the dairy buffalo: comparison with milk somatic cell count and bacteriological culture.a recently developed bovine cathelicidin (cath) elisa was evaluated in the dairy buffalo (bubalus bubalis) by testing 618 quarter milk samples from a herd with subclinical mastitis cases. somatic cell count (scc) and bacteriological culture (bc) were carried out on the same samples for comparison. out of 618 quarters, 258 (41.75%) were positive to cath, 289 (46.76%) had scc > 200,000 cells/ml, and 457 (73.95%) were positive to bc. the most prevalent microorganism was staphylococcus aureus (sau, ...202031783263
supplementation of l-tryptophan (an aromatic amino acid) in tris citric acid extender enhances post-thaw progressive motility, plasmalemma, mitochondrial membrane potential, acrosome, and dna integrities, and in vivo fertility rate of buffalo (bubalus bubalis) bull spermatozoa.the aromatic amino acid l-tryptophan is an essential and versatile molecule, acts by transferring an electron to free radicals and protects the plasma membrane from injuries. the aim of the present study was to investigate the effects of l-tryptophan in extender on semen quality parameters, in vitro longevity and in vivo fertility rate of buffalo spermatozoa during cryopreservation. two ejaculates were collected from each bull (n = 2 ejaculates and n = 4 bulls) with artificial vagina at 42 °c fo ...202031783000
genome-wide association studies for growth traits in buffaloes using the single step genomic blup.growth traits are important for the profitability of buffalo breeding systems, since in general, these animals are raised both for meat and milk. in this study, the single-step genomic blup method was employed to prospect the genomic regions' associated with weight at standard ages of 100, 210, 365, and 550 days in a buffalo population, aiming to identify genes with stronger expression for those characteristics. we found 6, 1, 2, and 5 snps significantly associated (p value < 10-5) with weight a ...202031673966
expression and functional role of bone morphogenetic proteins (bmps) in placenta during different stages of pregnancy in water buffalo (bubalus bubalis).the objective of this study was to document the expression and functional role of bmps in the placental (caruncle; car, cotyledon; cot) during different stages of pregnancy in water buffalo. samples collected from early pregnancy 1 (ep1); early pregnancy 2 (ep2), mid pregnancy (mp), late pregnancy (lp) while the third stage of oestrus cycle (np) was taken as control. also, the synergistic role of bmp4/bmp7 or combination on mrna expression of vwf, pcna, star, cyp11a1, 3βhsd, and bax were studied ...202031445010
multiple domestication of swamp buffalo in china and south east asia.the domestication of swamp buffalo (bubalus bubalis carabanesis) has been discussed for years and still remained unclear. to obtain a better understanding of where, when and how the swamp buffaloes were domesticated, the complete mitochondrial dna d-loop sequences of 1,788 individuals from china, vietnam, laos, thailand, burma, bangladesh and india were investigated. our results revealed swamp buffalo with abundant genetic diversity. the lineage sa of swamp buffalo may be first domesticated in l ...202031588618
polymorphisms in major histocompatibility complex genes and its associations with milk quality in murrah buffaloes.animal breeding programs have used molecular genetic tools as an auxiliary method to identify and select animals with superior genetic merit for milk production and milk quality traits as well as disease resistance. genes of the major histocompatibility complex (mhc) are important molecular markers for disease resistance that could be applied for genetic selection. the aim of this study was to identify single nucleotide polymorphisms (snps) and haplotypes in drb2, drb3, dma, and dmb genes in mur ...202031385169
bioinformatics analysis of candidate genes for milk production traits in water buffalo (bubalus bubalis).water buffalo (bubalus bubalis) is an important livestock species in developing countries due to its contribution to meat, milk production, and a certain form of labor. however, the genetic potential of buffalo milk production traits has not been fully exploited. to date, 516 candidate genes associated with milk production traits of buffalo have been identified. the present study aimed to explore the possible molecular mechanisms underlying milk production traits of this species through function ...202031321660
isolation and identification of bubaline herpesvirus 1 (buhv-1) from latently infected water buffalo (bubalus bubalis) from iran.in order to isolate buffaloes herpesvirus 1 (buhv-1) from latently infected water buffalo (bubalus bubalis), 16 buffalo heifers were selected from a herd. at first, animals were bled and their sera were tested by virus neutralization (vn) test, using bovine herpesvirus 1 (bohv-1). according to the results of vn test and dexamethasone injection (0.1 mg/kg bw) for 5 consecutive days, the examined buffaloes were divided into 4 groups. vaginal and nasal swabs were daily collected from all buffaloes ...202031313017
effect of cumulus cells of cumulus-oocyte complexes on in vitro maturation, embryonic developmental and expression pattern of apoptotic genes after in vitro fertilization in water buffalo (bubalus bubalis).in the present study, the potential of different grades of cumulus-oocyte complexes (cocs) for in vitro maturation (ivm) and embryonic development was assessed. further, the association of the expression pattern of anti-apoptotic mcl-1 and pro-apoptotic bax genes in embryonic development was analyzed. abattoir derived oocytes were graded into grade a and b based on surrounding cumulus rings. out of 1050 ovaries, a total number of 770 and 1360, were of grade a and b cocs, respectively, were aspir ...202030905313
evaluation of therapeutic potential of recombinant buffalo lactoferrin n-lobe expressed in e. coli.lactoferrin (lf) is a multifunctional bi-lobate iron-binding glycoprotein belonging to transferrin family with a mass of approximately 80 kd. being ubiquitously present in almost all biological secretions, it performs important biological functions. one of the earliest and very well-documented functions of lf is the antibacterial effect against broad spectrum gram-negative and gram-positive bacteria. in this study, buffalo lf n-lobe cdna was amplified, cloned and expressed as a fusion protein in ...202030773109
identification, molecular characteristics, and tissue differential expression of dgat2 full-cds cdna sequence in binglangjiang buffalo (bubalus bubalis).it has been found that diacylglycerol acyltransferase-2 (dgat2) plays a crucial role in the synthesis of triglycerides (tgs) in some mammals, but its role in buffalo lactation is unclear. in the present study, the dgat2 full-cds cdna sequence of binglangjiang buffalo was isolated, and the physicochemical characteristics and structure of its encoding protein were characterized. furthermore, the differential expressions of this gene in 10 tissues of lactating and non-lactating buffalo were analyze ...202032232120
heat stress modulates differential response in skin fibroblast cells of native cattle (bos indicus) and riverine buffaloes (bubalus bubalis).heat stress in hot climates is a major cause that negatively affects dairy animals, leading to substantial economic loss. the present study was aimed to analyze the effect of heat stress on cellular and molecular levels in dermal fibroblast of cattle and buffaloes. primary fibroblast culture was established using ear pinna tissue samples of cattle (bos indicus) and riverine buffaloes (bubalus bubalis). the cells were exposed to thermal stress at 42°c for 1 h and subsequently allowed to recover a ...202031994693
effect of animal age, postmortem chilling rate, and aging time on meat quality attributes of water buffalo and humped cattle bulls.the study was aimed to investigate the influence of animal age, post-slaughter chilling rate, and aging time on meat quality of m. longissimus dorsi (ld) of water buffalo (bubalus bubalis) and humped cattle (bos taurus indicus) bulls. after slaughtering, one side of carcasses was subjected to rapid chilling (rc) (0 ± 2°c) and other side was hanged in controlled room temperature (25 ± 2°c) for 3 hr, then allowed to the chiller (0 ± 2°c). the meat quality traits were analyzed at 1, 7, and 14 days ...202032789990
molecular characterization, tissue expression and polymorphisms of buffalo ppargc1a gene.ppargc1a exerts important functions in activating many nuclear receptors and transcription factors that are related to energy balance. previous studies have shown that ppargc1a gene is associated with lactation traits of dairy cattle. however, the functional role of the buffalo ppargc1a gene is still unknown. in this work, the complete coding sequence (cds) of buffalo ppargc1a was isolated and characterized for swamp and river buffalo. the cds length of ppargc1a for both types of buffalo was the ...202032775610
a comparative study on rumen ecology of water buffalo and cattle calves under similar feeding regime.in all, 12 male water buffalo (bubalus bubalis) calves and holstein (bos taurus) calves of similar age (10 ± 5 days) were selected to explore the mechanism underlying the differences in growth performance and feed conversion ratio between the two species. the experiment contains 33 days of sucking period and 40 days of post-weaning period. both calves were fed the same amounts of milk in sucking period, and starter and oat grass were supplied ad libitum both before and after the weaning period. ...202032657053
linkage disequilibrium-based inference of genome homology and chromosomal rearrangements between species.the aim of this study was to analyze the genomic homology between cattle (bos taurus) and buffaloes (bubalus bubalis) and to propose a rearrangement of the buffalo genome through linkage disequilibrium analyses of buffalo snp markers referenced in the cattle genome assembly and also compare it to the buffalo genome assembly. a panel of bovine snps (single nucleotide polymorphisms) was used for hierarchical, non-hierarchical and admixture cluster analyses. thus, the linkage disequilibrium informa ...202032434754
microrna-related markers associated with corpus luteum tropism in buffalo (bubalus bubalis).the study was undertaken to decipher the microrna (mirna) related markers associated with corpus luteum (cl) tropism in buffalo. the data obtained from deep sequencing of cl tissue from different physiological stages was mined in silico for the identification of mirna-related markers (ssr & snp). from the present study, 5 annotated and 176 unannotated mirna were deduced while comparing with bos taurus genome. in addition, 4 ssrs and 9 snps were deduced from the mirna sequences. these ssrs were o ...202030735793
identification of sarcocystis spp. in one-humped camels (camelus dromedarius) from riyadh and dammam, saudi arabia, via histological and phylogenetic approaches.sarcocystis(s.) spp. are intracellular protozoan parasites that infect birds and animals, resulting in substantial commercial losses. sarcocystis spp. have an indirect life cycle; canines and felines are known to act as final hosts, and numerous domestic and wild animals act as intermediate hosts. the presence of sarcocysts in camel meat may diminish its commercial quality. there is limited knowledge regarding the taxonomy and diagnosis of sarcocystis spp. that infect camels in saudi arabia. in ...202032605261
evolution and domestication of the bovini species.domestication of the bovini species (taurine cattle, zebu, yak, river buffalo and swamp buffalo) since the early holocene (ca. 10 000 bce) has contributed significantly to the development of human civilization. in this study, we review recent literature on the origin and phylogeny, domestication and dispersal of the three major bos species - taurine cattle, zebu and yak - and their genetic interactions. the global dispersion of taurine and zebu cattle was accompanied by population bottlenecks, w ...202032716565
phylogenetic and evolutionary analysis of hobi-like pestivirus: insights into origin and dispersal.the hobi-like pestivirus (hobipev), currently classified as pestivirus h species, is a pathogen associated with a broad spectrum of clinical manifestations in ruminants, particularly in cattle. since hobipev complete genome sequencing data is scarce, in the present study we described five nearly complete new brazilian hobipev genomes and further perform a more complete genetic and evolutionary characterization with all additional genome sequences available in the genbank database. entropy and se ...202032073749
toxocara vitulorum infection in a yak (bos mutus grunniens) calf from tyrol (austria).the domestic yak bos mutus grunniens is an important livestock animal in parts of asia, especially of the himalayan region, where people rely on it for meat, wool, milk and labour. in its countries of origin, the yak is commonly infected with the ascarid toxocara vitulorum. this parasite mainly infects cattle (bos taurus) and domestic buffalo (bubalus bubalus) and is most commonly found in sub-tropical regions, but has been occasionally reported in more temperate climates, including several cent ...202032057397
comparison of ruminal digestibility of origanum onites l. leaves in dairy buffalo and cows.this experiment evaluated the ruminal digestibility of turkish oregano leaves in dairy buffalo and cows. ruminally cannulated, multiparous brown swiss cows (n = 3) and water buffalo (bubalus bubalis; n = 3) were used in the experiment. the ad libitum basal diet was balanced to nrc requirements for a dry, nonpregnant multiparous dairy cow (680 kg) and consuming 12.8 kg of dm/day. air-dried, ground, weighed oregano leaves were inserted in the rumen of all animals before the morning feeding within ...202032030523
short communication: characterization of the milk protein expression profiles in dairy buffaloes with and without subclinical mastitis.the aim of this study was to characterize the proteins present in milk whey from buffaloes with and without subclinical mastitis using a proteomic approach to identify differentially expressed proteins as potential biomarkers for this disease. whey from murrah buffaloes with subclinical mastitis was compared with whey from healthy animals using liquid chromatography-tandem mass spectrometry. the annotated protein databases for bubalus bubalis and bos taurus were used in the analysis, and the gen ...202031954559
genome-wide association study applied to type traits related to milk yield in water buffaloes (bubalus bubalis).this research aimed to estimate genetic parameters for milk yield and type traits [withers height (wh), croup height (ch), body length (bl), croup length (cl), iliac width (ilw), ischial width (isw), and thoracic circumference] in murrah buffaloes and to identify genomic regions related to type traits by applying a single-step genome-wide association study. data used to estimate the genetic parameters consisted of 601 records of milk yield in the first lactation and the aforementioned type trait ...202031759604
cryptosporidium and giardia in feral water buffalo (bubalus bubalis) in the south east arnhem land indigenous protected area, australia.global investigations have implicated water buffalo (bubalus bubalis) as a potential source of zoonotic cryptosporidium and giardia parasites which may pose a threat to human health. in australia, buffalo are a feral pest that have colonised the floodplains, wetlands and woodlands of indigenous owned and managed arnhem land, in tropical monsoonal northern australia. indigenous people from the remote community ngukurr have raised concerns about the potential threat to their health from shared use ...202032424553
analysis of meiotic segregation by triple-color fish on both total and motile sperm fractions in a t(1p;18) river buffalo bull.chromosomal aberrations are relatively frequent pathologies in both humans and animals. among them, translocations present a specific meiotic segregation pattern able to give a higher percentage of unbalanced gametes that can induce fertility problems. in this study, the meiotic segregation patterns of 1p, 1q and 18 bubalus bubalis chromosomes were analyzed in both total sperm fraction and motile sperm fraction of a t(1p;18) carrier and a control bulls by triple-color fish analysis with a pool o ...202032365118
bovine papillomavirus type 2 infection associated with papillomatosis of the amniotic membrane in water buffaloes (bubalus bubalis).multiple papillomatous nodules were observed scattered over the amniotic membrane in six water buffaloes that had recently aborted. grossly, some of the nodules had multiple villous projections while others appeared as single prominent conical or cylindrical horns. histology revealed folded hyperplastic and hyperkeratotic epithelium supported by a narrow fibro-vascular stalk. using pcr, sequences of the bovine deltapapillomavirus type 2 (bpv-2) e5 gene were amplified from the amniotic papillomas ...202032260380
additional characterization of the adult worm mammomonogamus laryngeus (railliet, 1899) and the tissue lesions caused by the infection in buffaloes.mammomonogamus spp. are parasites with curious characteristics, such as the "y" shape that results from male and female maintaining the permanent copulation position and the controversial presence or absence of spicules. these nematodes are hematophagous and cause damage to the upper respiratory tracts of cattle, sheep, goats, deer, wild yaks, and orangutans. human infection is rare and most cases until now have been in the caribbean islands or in brazil, and mainly in farmworkers but recently t ...202032544763
whole genome re-sequencing reveals adaptation prior to the divergence of buffalo subspecies.the application of high throughput genotyping or sequencing data helps us to understand the genomic response to natural and artificial selection. in this study, we scanned the genomes of five indigenous buffalo populations belong to three recognized breeds, adapted to different geographical and agro-ecological zones in iran, to unravel the extent of genomic diversity and to localize genomic regions and genes underwent past selection. a total of 46 river buffalo whole genomes, from west and east ...202033179728
assessment of multicolor flow cytometry panels to study leukocyte subset alterations in water buffalo (bubalus bubalis) during bvdv acute infection.the identification of cross-reactive monoclonal antibodies (mabs) that recognize orthologous leukocyte differentiation molecules (ldm) in buffaloes has overcome a major impediment limiting research on the immune response to pathogens and development of vaccines. as reported, two pilot trials were conducted to accomplish two objectives: (1) demonstrate that multiparameter flow cytometry can be conducted equally well in buffalo with mabs directly and indirectly labeled with fluorochromes in resear ...202033178728
determination and ecological risk assessment of two endocrine disruptors from river buffalo, south africa.4-tert-octylphenol (4-top) and triclosan (tcs) are endocrine disruptors which have been detected in environmental matrices such as air, soil and water at ultra-low levels. exposure to endocrine disruptors may account at least in part, for the global increase in the incidence of non-communicable diseases like cancers and diabetes and may also lead to an imbalance in the aquatic ecosystem. river buffalo is an important natural resource in the eastern cape of south africa serving more than half a m ...202033155083
optimization and comparison of three-dimensional culture conditions in different media of coculture and encapsulation system for in vitro follicular development in bubalus bubalis.the establishment of an in vitro culture system for complete oocyte maturation from the early stages of ovarian follicles is still a challenge. the aim of the present study was to assess the effect of different matrix with different culture media on the developmental growth of ovarian follicles in vitro. an ovarian histoarchitectural study was carried out to identify the primordial (0.027-0.039 mm), primary (0.041-0.079 mm), small preantral (0.085-0.131 mm), large preantral (0.132-0.294 mm), sma ...202033147076
fsh stimulation with short withdrawal improves oocyte competence in italian mediterranean buffalo (bubalus bubalis).the aim of this work was to evaluate the efficiency of different fsh doses and fsh coasting times before ovum pick-up (opu) on follicular growth and oocyte competence in buffalo. experiment 1 involved two different fsh treatments: 40 mg fsh given three (fsh3) or six (fsh6) times, 2 days after dominant follicle removal were tested, with opu carried out after 40-44 h of coasting. in experiment 2, opu was carried out after fsh6 protocol followed by 28-32 h (c1), 40-44 h (c2), or 64-68 h (c3) of coa ...202033143113
integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (bubalus bubalis).in mammals, sperm need to mature in the epididymis to gain fertilization competency. however, the molecular mechanism underlying buffalo sperm maturation remains elusive. exploring sperm physiology at the posttranslational modification (ptm) level could help to develop our understanding of these mechanisms. protein phosphorylation and ubiquitination are major ptms in the regulation of many biological processes. in the present study, to our knowledge, we report the first phosphoproteome and ubiqu ...202033140506
synthesis of iron oxide nanoparticles-antiubiquitin antibodies conjugates for depletion of dead/damaged spermatozoa from buffalo (bubalus bubalis) semen.the synthesis of iron oxide nanoparticles (ionps)-antiubiquitin antibodies (abs) complex for depletion of dead/damaged spermatozoa from buffalo semen was done. the ionps synthesized were round in shape with size of 12.09 ± 0.91 nm. at the end of the two-step functionalization, that is, silanization and pegylation of bare ionps and bioconjugation of functionalized iopns, particles with the sizes of 19.15 ± 1.46, 20.72 ± 0.95, and 73.01 ± 7.56 nm, respectively, were obtained. twenty-four semen sam ...202033135803
intra-articular injection techniques of the buffalo (bubalus bubalis) hindlimb digit using anatomical and contrast arthrography-guided landmarks.this study was designed to evaluate and compare the optimal sites for intra-articular (ia) injection into the digits of buffalo by discrimination of the injection criteria. forty-eight cadaveric hind digits of adult buffalos and nine live ones were assigned for three trial investigations. in the first division, eighteen sound cadaveric limbs were used to describe the anatomical features of the hind digit. in the second division, thirty cadaveric limbs (ten for each approach) were injected with a ...202033128523
contrasting population genetics of co-endemic cattle- and buffalo- derived theileria annulata.a study was designed to improve understanding of the genetics of theileria annulata populations in sympatric cattle and asian buffalo (bubalus bubalus). the study was undertaken in the punjab province of pakistan, where the prevalence of tropical theileriosis is high. parasite materials were collected from infected animals in defined regions, where cattle and asian buffalo are kept together. six satellite dna markers and a mitochondrial cytochrome b marker were used to explore the multiplicity o ...202033113478
comparative transcriptome analysis reveals that pck1 is a potential gene affecting imf deposition in buffalo.in china, although buffaloes are abundant, beef is mainly obtained from cattle, and this preference is mainly attributed to the low intramuscular fat (imf) content of buffalo. genetic factors are an important driver that affects imf deposition.202033045988
reducing ruminal ammonia production with improvement in feed utilization efficiency and performance of murrah buffalo (bubalus bubalis) through dietary supplementation of plant-based feed additive blend.the study evaluated the potential of blends of eucalyptus oil and aqueous extract of mulethi (root of glycyrrhiza glabra) to reduce rate of ruminal ammonia production without affecting feed digestion to improve nitrogen utilization efficiency and performance of murrah buffalo (bubalus bubalis). based on preliminary independent studies with graded doses of eucalyptus oil and mulethi root aqueous extract in modulating in vitro rumen fermentation, four blends of feed additive comprising graded dose ...202033015136
innovative use and efficiency test of subcutaneous transponders for electronic identification of water buffaloes.the objective of this study was to evaluate the viability of using transponders for the electronic identification of water buffaloes and compare their efficiency when used in animals of different age groups. electronic transponders with rfid technology (2.1 × 12.2 mm) were implanted subcutaneously (d0) in the scutiform cartilage. the animals consisted of four groups: clf-i (17 calves; 2.1 ± 1.9 months), clf-ii (20 calves; 5.1 ± 3.2 months), hfr (20 heifers; 22 ± 4.7 months) and str (19 steers; 2 ...202033009587
antibiotic resistance and genotyping of meca-positive methicillin-resistant staphylococcus aureus (mrsa) from milk and nasal carriage of dairy water buffaloes (bubalus bubalis) in the philippines.mastitis is considered as an economically important disease of dairy buffaloes in asia. this study examined the mastitis milk and nasal swab samples for the detection and genotyping of methicillin-resistant staphylococcus aureus (mrsa) in water buffaloes.202033005664
parthenogenetic activation of buffalo (bubalus bubalis) oocytes: comparison of different activation reagents and different media on their developmental competence and quantitative expression of developmentally regulated genes.this study was carried out to compare the efficacy of different methods to activate buffalo a + b and c + d quality oocytes parthenogenetically and to study the in vitro developmental competence of oocytes and expression of some important genes at the different developmental stages of parthenotes. the percentage of a + b oocytes (62.16 ± 5.06%, range 53.8-71.3%) was significantly higher (p < 0.001) compared with that of c + d oocytes (37.8 ± 5.00%, range 28.6-46.1%) retrieved from slaughterhouse ...202033004105
granulosa cell-conditioned medium enhances steroidogenic competence of buffalo (bubalus bubalis) theca cells.granulosa cells (gcs) and theca cells (tcs) are the main components of follicles, and the interactions between gcs and tcs play a significant role in steroidogenesis, follicular growth, and atresia. however, the effects of gcs in the form of conditioned medium on steroidogenesis in buffalo tcs remain unclear. in the present study, the impacts of gc-conditioned medium (gccm) on androgen synthesis in buffalo tcs were examined. the results showed that gccm collected at 48 h promoted both the expres ...202033000385
novel targets identified by integrated proteomic and phosphoproteomic analysis in spermatogenesis of swamp buffalo (bubalus bubalis).to understand mechanisms of spermatogenesis, the proteome and the phosphoproteome in prepubertal and pubertal swamp buffalo (bubalus bubalis) testes were analyzed using tandem mass tag (tmt) coupled with liquid chromatography-tandem mass spectrometry (lc-ms/ms). in prepubertal testes, 80 proteins were overexpressed, 148 proteins were underexpressed, and 139 and 142 protein sites had higher and lower phosphorylation, respectively, compared to the levels in pubertal testes. several of these protei ...202032973212
ovarian dynamics of buffalo (bubalus bubalis) synchronized with different hormonal protocols.the study evaluates the effect of three hormonal protocols on ovarian dynamics and progesterone (p4) secretion of buffalo (bubalus bubalis). twenty-nine pluriparous murrah buffaloes were used. the protocols were as follows: ovsynch (n = 10): 100 μg of gonadorelin (day 0), 500 μg of cloprostenol (day 7), and 100 μg of gonadorelin (day 9). cidr+eb (intravaginal device (cidr®) + estradiol benzoate; n = 10): cidr plus 2 mg of eb (day 0), withdrew of cidr, 500 μg of cloprostenol (day 7) and 1 mg of e ...202032949356
spermatozoa produced during winter are superior in terms of phenotypic characteristics and oviduct explants binding ability in the water buffalo (bubalus bubalis).although reduced reproductive efficiency during summer has been well documented in buffaloes, the reason for the same is yet to be understood. the present study was conducted to identify the subtle differences in sperm phenotypic characteristics (motility, membrane integrity, acrosome reaction and lipid peroxidation status), oviduct binding ability and expression of fertility-associated genes (ak 1, atp5d, catsper 1, cytochrome p450 aromatase, spp1 and pebp1) between winter and summer seasons in ...202032945545
evaluation of the immune responses against reduced doses of brucella abortus s19 (calfhood) vaccine in water buffaloes (bubalus bubalis), india.brucella abortus s19 is the most widely used vaccine for the prevention of bovine brucellosis which remains the reference vaccine to which many other vaccine/s are compared. considering the larger vaccination coverage by reduced dose of vaccine, the study aimed to compare reduced graded doses (1/10th, 1/20th and 1/100th) with standard dose of s19 vaccine (40 × 109cfu /dose) to determine the effective immunizing dose in water buffaloes.202032943264
effect of basic fibroblast growth factor (fgf2) on cumulus cell expansion, in vitro embryo production and gene expression in buffalo (bubalus bubalis).the present study was undertaken to evaluate the effect of different concentration of fgf2 viz. 5 ng (t1), 10 ng (t2), and 20 ng/ml (t3) on cumulus cell expansion, oocyte maturation, in vitro embryo production, total cell number (tcn) of the blastocyst, and expression of the fgf2 and fgfr2 transcripts in buffalo oocytes and the embryos. results showed that the effect of fgf2 on the diameter of buffalo coc was significantly higher (p < 0.05) in the t1 group than the other groups at 24h of maturat ...202032921625
selection and validation of reference genes for rt-qpcr in adipose and longissimus dorsi muscle tissues of buffalo.real-time quantitative pcr (rt-qpcr) is widely used to measure and evaluate gene expression. the precision and reliability of rt-qpcr are critically dependent on the selection of suitable reference genes (rgs). in this study, an effort was made to identify the optimal rgs for rt-qpcr analysis of adipose and the longissimus dorsi muscle (lm) in buffaloes. rna sequencing data were firstly analyzed to obtain 10 candidate genes (fkbp1a, c25h16orf72, pnrc2, iqgap1, atp5pd, rpl6, ndufb4, tra2a, caprin ...202032862779
identification of genomic regions related to age at first calving and first calving interval in water buffalo using single-step gblup.in brazil, water buffaloes have been used to produce milk for mozzarella cheese production. consequently, the main selection criterion applied for the buffalo genetic improvement is the estimated mozzarella yield as a function of milk, fat and protein production. however, given the importance of reproductive traits in production systems, this study aimed to use techniques for identifying genomic regions that affect the age at first calving (afc) and first calving interval (fci) in buffalo cows a ...202032853485
a review of toxoplasmosis and neosporosis in water buffalo (bubalus bubalis).toxoplasmosis and neosporosis are diseases with worldwide distribution that are associated with reproductive problems in livestock and responsible for economic losses. this review presents an overview of the current knowledge relative to these diseases in water buffalo (bubalus bubalis). in general, buffalo are considered resistant to clinical toxoplasmosis because there are studies only reporting serological evidence of natural infection in these animals. studies have described age, poor hygien ...202032851033
effect of dickkopf-1 and colony stimulating factor-2 on the developmental competence, quality, gene expression and live birth rate of buffalo (bubalus bubalis) embryos produced by hand-made cloning.a functional canonical wnt signaling pathway exists in preimplantation embryos and inhibits embryonic development. recent studies suggest that this pathway is over-expressed in nuclear transferred (nt), compared to ivf embryos. the present study investigated the effects of dickkopf-1 (dkk1), an inhibitor of canonical wnt signaling pathway and colony stimulating factor-2 (csf2), an embryokine, on the developmental competence, quality, gene expression and live birth rate of nt buffalo embryos prod ...202032823021
semen parameters and fertility potency of a cloned water buffalo (bubalus bubalis) bull produced from a semen-derived epithelial cell.semen contains epithelial cells that can be cultured in vitro. for somatic cell nuclear transfer applications, it is essential to know whether clone(s) produced from semen-derived epithelial cells (sedecs) are healthy and reproductively competent. in this study, the semen and fertility profile of a cloned bull (c1) that was produced from a sedec were compared with its donor (d1) and with two cloned bulls (c2, c3) that were produced from commonly used skin-derived fibroblast cells (skdfcs). we ob ...202032822364
sequencing and assembly of the egyptian buffalo genome.water buffalo (bubalus bubalis) is an important source of meat and milk in countries with relatively warm weather. compared to the cattle genome, a little has been done to reveal its genome structure and genomic traits. this is due to the complications stemming from the large genome size, the complexity of the genome, and the high repetitive content. in this paper, we introduce a high-quality draft assembly of the egyptian water buffalo genome. the egyptian breed is used as a dual purpose animal ...202032813723
investigation of the role of aromatic residues in the antimicrobial peptide bucathl4b.antimicrobial peptides (amps) are an attractive alternative to traditional small molecule antibiotics as amps typically target the bacterial cell membrane. a trp-rich peptide sequence derived from water buffalo (bubalus bubalis), bucathl4b was previously identified as a broad-spectrum antimicrobial peptide.202032798369
seasonal effects on mirna and transcriptomic profile of oocytes and follicular cells in buffalo (bubalus bubalis).season clearly influences oocyte competence in buffalo (bubalus bubalis); however, changes in the oocyte molecular status in relation to season are poorly understood. this study characterizes the microrna (mirna) and transcriptomic profiles of oocytes (oos) and corresponding follicular cells (fcs) from buffalo ovaries collected in the breeding (bs) and non-breeding (nbs) seasons. in the bs, cleavage and blastocyst rates are significantly higher compared to nbs. thirteen mirnas and two mrnas show ...202032782284
remote monitoring system as a tool for calving management in mediterranean buffalo heifers (bubalus bubalis).buffalo breeding is common in southern italy. dystocia compromises dam's and newborn health and welfare. difficult parturition could be solved through prompt calving assistance, even if the identification of the beginning of delivery is challenging. herein, we aimed to evaluate a remote calving alarm system in 15 mediterranean buffalo heifers. an intravaginal probe was placed close to the external cervical os once premonitory signs of delivery were observed. no vaginal discharge nor signs of dis ...202032780888
escherichia coli membrane-derived oxygen-reducing enzyme system (oxyrase) protects bubaline spermatozoa during cryopreservation.the objective of this study was to determine the effectiveness of deoxygenation of semen extender using escherichia coli membrane-derived oxygen scavenger (oxyrase) on post-thaw quality of buffalo (bubalus bubalis) spermatozoa. sixteen semen ejaculates, four each from four bulls, were each divided into five equal fractions, diluted using tris-egg yolk extender supplemented with different concentrations of oxyrase (0, 0.3, 0.6, 0.9, and 1.2 u/ml), designated as treatments t1, t2, t3, t4, and t5, ...202032780495
theca cell-conditioned medium added to in vitro maturation enhances embryo developmental competence of buffalo (bubalus bubalis) oocytes after parthenogenic activation.theca cells (tcs) play a key role in follicular growth and atresia. tcs synthesize androgens that act as substrate for granulosa cells (gcs) aromatization to estrogens needed for oocyte maturation. however, the effects of tcs in the form of conditioned medium on in vitro maturation (ivm) and developmental competence of buffalo oocytes remain unclear. in the present study, we examined the impacts of tc-conditioned medium (tccm) on maturation efficiency and embryo development of buffalo oocytes af ...202032767798
feeding preferences and nutritional niche of wild water buffalo (bubalus arnee) in koshi tappu wildlife reserve, nepal.the nutritional characteristics of food resources play an important role in the foraging behavior of animals and can provide information valuable to their conservation and management. we examined the nutritional ecology of wild water buffalo (bubalus arnee; hereafter "buffalo") in the koshi tappu wildlife reserve of nepal during autumn using a multidimensional nutritional niche framework. we identified 54 plant species as being foraged by buffalo. we found that buffalo consumed graminoids and fo ...202032760500
theca cell-conditioned medium enhances steroidogenesis competence of buffalo (bubalus bubalis) granulosa cells.theca cells (tcs) play a crucial role in follicular development and atresia. tcs synthesize the androgens that act as substrate for granulosa cells (gcs) aromatization to estrogens needed for follicular growth. however, the effects of tcs in the form of conditioned medium on steroidogenesis in buffalo gcs remain unclear. in the present study, the impacts of tc-conditioned medium (tccm) on estrogen synthesis in buffalo gcs were examined. the results showed that tcs secreted principally testostero ...202032748525
diversity analysis at mhc class ii dqa locus in buffalo (bubalus bubalis) indicates extensive duplication and trans-species evolution.variation at mhc class ii-dqa locus in riverine and swamp buffaloes (bubu) has been explored in this study. through sequencing of buffalo dqa, 48 nucleotide variants identified from 17 individuals, reporting 42 novel alleles, including one pseudogene. individual animal displayed two to seven variants, suggesting the presence of more than two bubu-dqa loci, as an evidence of extensive duplication. dn values were found to be higher than ds values at peptide binding sites, separately for riverine a ...202032738270
expression pattern of glut 1, 5, 8 and citrate synthase transcripts in buffalo (bubalus bubalis) preimplantation embryos produced in vitro and derived in vivo.in vitro-produced (ivp) embryos are reported to be developmentally lesser competent than in vivo-derived (ivd) embryos and supposed to differ in the expression of genes related with glucose metabolism. so, the present study was conducted to analyse the expression pattern of glut 1, 5, 8 and citrate synthase (cs) in oocytes and embryos produced in vivo or in vitro in buffalo. ivd embryos were obtained from 18 superovulated buffaloes. ivp embryos were obtained from slaughterhouse-derived oocytes s ...202032706414
molecular diagnosis and prevalence of trypanosoma vivax (trypanosomatida: trypanosomatidae) in buffaloes and ectoparasites in the brazilian amazon region.trypanosoma vivax ziemann is a parasite that affects both wild and domestic ungulates and is transmitted mechanically via tabanids and other blood-sucking insects in the americas. a total of 621 blood samples from water buffaloes (bubalus bubalis (linnaeus) (artiodactyla: bovidae), and 184 ectoparasite samples (amblyomma cajennense (fabricius) sensu stricto and rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae), and haematopinus tuberculatus (burmeister) (phthiraptera: haematopin ...202032696974
influence of  uterine torsion in water buffaloes (bubalus bubalis) with insights into the hematological and biochemical prognostic values regarding to manual correction.bovine uterine torsion refers to the rotation of the gravid uterus around its longitudinal axis. it is the dominant cause of dystocia in buffaloes. a total of two hundred and seventeen dystocia's egyptian buffaloes were examined, and another twenty were apparently normal at the full term (healthy pregnant group). from the dystocia's buffaloes, twenty-three percentage (n = 50) was uterine torsion after gynecology inspection at presentation (ut group) and 5 days after the manual correction (utc gr ...202032677006
activation of wnt/β-catenin signaling pathway enhances the derivation of buffalo (bubalus bubalis) embryonic stem cell-like cells.wnt/β-catenin signaling pathway plays an important role in maintaining self-renewal and pluripotency of human and mouse embryonic stem cells (escs). activation of wnt/β-catenin signaling pathway by glycogen synthase kinase-3 (gsk3) inhibitor, the wnt signaling agonist, could maintain the pluripotency of human and mouse escs in the presence of serum. however, the role of signaling pathway in the derivation of buffalo escs remains unclear. in this study, we used gsk3 inhibitors (6-bromoindirubin-3 ...202032673062
early prediction of corpus luteum functionality using an imaging software.the present study aimed to assess the applicability of luteal blood flow data acquired through the use of color doppler ultrasonography and a post-processing analysis tool (imagej) for predicting pregnancy in buffaloes (bubalus bubalis). the experiment was carried out on 59 multiparous italian mediterranean buffaloes that underwent synchronization of estrus and fixed-time artificial insemination (tai). corpus luteum features (size: cls and blood flow: bfa) were taken from day 5 to 10 after tai a ...202032626727
passive immunity transfer in water buffaloes (bubalus bubalis).this study aimed to evaluate passive immunity transfer in healthy buffalo calves. colostrum samples from heifers (without previous calving) and primiparous and pluriparous dams and blood samples from their offspring were obtained at calving, before colostrum intake, and at 24, 48, and 72 h after calving for determination of serum activities of gammaglutamyltransferase and alkaline phosphatase and serum concentrations of total protein (tp), immunoglobulin a (iga) and igg, and lactoferrin. the res ...202032626726
effect of synergism between carboxylated poly-l-lysine and glycerol on freezability of nili-ravi buffalo (bubalus bubalis) semen.carboxylated poly-l-lysine (cpll), an ampholytic polymer, has remarkable cryoprotective properties. it was hypothesized that cpll will reduce/replace glycerol in extender and improve the freezability of buffalo semen. the objective was to evaluate various combinations of cpll and glycerol in extenders for any synergism toward cryosurvivability of nili-ravi buffalo sperm. semen collected from four nili-ravi buffalo bulls (ejaculates = 40; replicates = 5) was diluted in tris-citric acid based exte ...202032609000
caffeic acid improves microscopic sperm parameters and antioxidant status of buffalo (bubalus bubalis) bull semen following freeze-thawing process.this study investigates the effects of cryopreservation and supplementation of buffalo's semen with caffeic acid. it studies the effects of different caffeic acid concentrations on cryopreservation capacity of the buffalo and evaluates their influence on various sperm parameters like motility, viability, progressive motility, sperm plasma membrane integrity, and antioxidant status. twenty-four semen samples were collected with an artificial vaginal from three adult water buffalos. the semen samp ...202032590017
first record of natural infection with anaplasma marginale in sucking lice infesting the water buffalo (bubalus bubalis) in mexico.the water buffalo (bubalus bubalis) is an asian species of bovine which was introduced in mexico in 1992, as an alternative for milk and meat production. however, little is known about its associated ectoparasites, in particular about their lice. as a part of a project to identify the diversity and prevalence of anaplasma in cattle and water buffaloes in the state of veracruz, mexico, we visited a ranch in the downtown area in which 42 buffaloes were visually inspected for the presence of ectopa ...202032588174
high trypanosoma vivax infection rates in water buffalo and cattle in the brazilian lower amazon.highly sensitive and accurate molecular diagnostic methods have not yet been employed for livestock trypanosomosis in the brazilian lower amazon although the first reports of trypanosoma vivax and trypanosoma evansi in brazil were in water buffalo (bubalus bubalis) in this region. the present study assessed trypanosomosis in buffalo and cattle raised in communal and seasonally flooding pastures in the state of pará using the fluorescent fragment length barcoding (fflb) method. t. evansi was not ...202032565339
extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes.extracellular vesicles (evs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. the aim of the present report was to provide the first isolation, quantification and protein characterization of evs from buffalo (bubalus bubalis) sera infected with theileria spp.202032528536
identification of pathogenic leptospira species in the urogenital tract of water buffaloes (bubalus bubalis) from the amazon river delta region, brazil.in the current context of deforestation and fire in the amazon, buffaloes could be a cost-effective and sustainable alternative for cattle production in the region, as they can convert low-quality foods and be raised in floodplain areas. however, little is known about the reproductive diseases that affect these animals; thus, the purpose of this study was to perform the molecular characterization of leptospira spp. in the urogenital tract of water buffaloes (bubalus bubalis) raised in the amazon ...202032478114
rumen fluke, fischoederius elongatus (trematoda: gastrothylacidae): preliminary investigation of suitable conditions for egg hatching.various temperatures may have different effects on the distribution of paramphistomes that cause amphistomosis in cattle, including fischoederius elongatus. therefore, this study aimed to investigate the effects of different temperature treatments on f. elongatus hatching, with specific identification using morphological, histological, and phylogenetic analysis. all specimens were collected from two buffalo (bubalus bubalis) rumens in a slaughterhouse in pathum thani province, thailand. f. elong ...202032470871
ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (bubalus bubalis) testis.protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. although several ubiquitinated proteins have been found in buffalo (bubalus bubalis) testis in our previous studies, large-scale profiling of buffalo testis ubiquitome has not been reported to date. in the present study, we first identified a global profiling of lysine ubiquitination of adult buffalo testis using a highly sensitive ...202032469046
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