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development of cathepsin-l cysteine proteinase based dot-enzyme-linked immunosorbent assay for the diagnosis of fasciola gigantica infection in buffaloes.native cathepsin-l cysteine proteinase (28 kda) was purified from the excretory secretory products of fasciola gigantica and was used for sero-diagnosis of f. gigantica infection in buffaloes by dot-enzyme-linked immunosorbent assay (dot-elisa). the test detected f. gigantica field infection in these animals with a sensitivity of ∼ 90%. no specific igg antibody binding was displayed by sera obtained from 76 buffaloes considered to be fasciola and other parasite-free by microscopic examination of ...201222055612
anthelmintic effect of a methanol extract of leaves of dregea volubilis on paramphistomum explanatum.dregea volubilis (family asclepediaceae) is widely used as anthelmintic in traditional system of medicine in eastern and southern part of india. the aim of this study was to evaluate the anthelmintic activity of the methanol extract of d. volubilis leaves (medv) and to observe its effect through sem study. live parasites (trematode paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline (pbs). it was incubated at 37?±?1°c in media containing either no extract (co ...201121789584
anthelmintic effect of a methanol extract of bombax malabaricum leaves on paramphistomum explanatum.bombax malabaricum (family bombacaceae) is used as anthelmintic in traditional system of medicine in southern punjab of pakistan. the objective of this study was to evaluate the anthelmintic activity of the methanol extract of b. malabaricum leaves (mebm). live parasites (trematode: paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline. it was incubated in petri dishes at 37 ± 1°c in media containing either no extract (control) or mebm, the test drug at 10, 25, ...201121853226
fasciola gigantica: production and characterization of a monoclonal antibody against recombinant cathepsin b3.a number of monoclonal antibodies (moabs) against a recombinant cathepsin b3 (rcatb3) of fasciola gigantica were produced in balb/c mice. reactivity and specificity of these moabs were assessed by indirect elisa and immunoblotting techniques. six stable clones, namely 1c4, 1e9, 2e5, 2f9, 5b4, 5d7 were obtained. all moabs reacted with rcatb3 at molecular weight (mw) 37 kda as well as the glycosylated peptide at 55-75 kda and with the native catb3 at mw 37 kda in wb extracts of metacercariae (met) ...201120736007
evaluation of medicinal plant extracts and isolated compound epicatechin from ricinus communis against paramphistomum cervi.the purpose of this study is to determine the efficacies of hexane, chloroform, ethyl acetate, acetone, and methanol leaf extracts of euphorbia hirta l., psidium guajava l., ricinus communis l., solanum trilobatum l., and tridax procumbens l. against sheep fluke paramphistomum cervi (digenea: paramphistomatidae). all plant extracts showed moderate effects after 24-áh of exposure; however, the highest parasite mortality was found in the methanol extract of r. communis. in the present study, bioas ...201121842382
evaluation of medicinal plant extracts against ticks and fluke.the present study was based on assessments of the antiparasitic activities to determine the efficacies of leaf hexane, chloroform, ethyl acetate, acetone and methanol extracts of aegle marmelos (linn.) correa ex roxb, andrographis lineata wallich ex nees., andrographis paniculata (burm.f.) wallich ex nees., cocculus hirsutus (l.) diels, eclipta prostrata l., and tagetes erecta l. against the adult cattle tick haemaphysalis bispinosa neumann 1897 (acarina: ixodidae), the larvae of rhipicephalus ( ...201020922419
infections with gastrointestinal nematodes, fasciola and paramphistomum in cattle in cambodia and their association with morbidity parameters.prevalence and seasonal variations of helminth infections and their association with morbidity parameters were studied in traditionally reared cambodian cattle. four villages in two provinces of west cambodia were visited on monthly intervals over a period of 11 months, during which 2391 animals were faecal and blood sampled for parasitological and haematological examinations. the body condition score (bcs), faecal consistency (diarrhoea score, ds), colour of the ocular conjunctivae (famacha(©)) ...201021071148
molecular characterization of the its-2 fragment of paramphistomum leydeni (trematoda: paramphistomidae).paramphistomosis has shown an increased spread over the last years in argentina, being in some regions an emerging parasitosis, which has motivated their study and identification. even when morphological features were reported, molecular characterization appears as a complementary and reliable tool. samples of paramphistomum leydeni collected since 2002 from different origin, definitive hosts, natural or experimental infections and preservation method were subjected to pcr-rflp for its2+ fragmen ...201021145661
the performance of a pcr assay for field studies on the prevalence of fasciola hepatica infection in galba truncatula intermediate host snails.the aim of this work was to develop a pcr assay for the detection of f. hepatica in galba truncatula snails and to evaluate its performance in field studies. primers were designed to amplify a 124bp non-coding tandem repeat found in the fasciola genome. the result was a banding pattern corresponding to multiples of the initial target sequence. the sensitivity of the pcr was determined on experimentally infected snails. the test was sensitive enough to detect fluke dna in snails experimentally in ...201019939569
major lipids and fatty acids in the liver and rumen fluid of the goat ( capra hircus) infected with the trematode paramphistomum cervi.the present study records the occurrence of major lipid fractions and their fatty acids in a digenetic trematode parasite paramphistomum cervi, and the rumen fluid and liver of the goat (capra hircus). the amount of neutral lipids (nl), glycolipids (gl) and phospholipids (pl) of goat liver, rumen fluid and of the parasite shows that the rumen fluid is rich in nl, which is also in maximum quantity in the parasite, while the liver is rich in pl followed by nl. the number of fatty acids of total li ...201020831844
paramphistomum cervi: surface topography of the tegument of adult fluke.adult paramphistomum cervi or rumen fluke are pear-shaped, slightly concave ventrally and convex dorsally. the worm measures about 5-13 mm in length and 2-5 mm in width across the mid-section. as observed by scanning electron microscopy (sem), the tegumental surface in all part of the body, appears highly corrugated with transverse folds alternating with grooves and is spineless. at high magnification, the surface of the fold is composed of microfolds or ridges separated by microgrooves or pits. ...201020045698
characterization and localization of saposin-like protein-2 (sap-2) in fasciola gigantica.fasciola gigantica saposin-like protein-2 (fgsap-2) belongs to a family of lipid-interacting proteins that are involved in the cytolysis of target cells. in this study, we have cloned and expressed fgsap-2 and produced the antibody against this recombinant protein. rabbit antiserum against rfgsap-2 reacted with a similar native protein in the whole body extracts of the 4-week-old juvenile and adult stage, as well as a protein in their excretion-secretion, but not in the tegument. in situ hybridi ...201021188603
prevalence and intensity of paramphistomum daubneyi infections in cattle from north-eastern algeria.parasitological investigations on ruminal paramphistomosis were carried out in 2033 cattle over a 14-month period in three algerian slaughterhouses to determine the prevalence and intensity of this disease. the prevalence of infection varied from different slaughterhouses (1.2% at el khroub, 7.5% at ferdjioua and 12.1% at jijel). the worm burden was significantly higher in cattle from el khroub than for those slaughtered in the other two sites (a mean of 984.1 parasites compared with 87.5 and 14 ...201019732472
an optimized dna extraction and multiplex pcr for the detection of fasciola sp. in lymnaeid snails.this study deals with the development and validation of an original pcr protocol to assess the presence of fasciola hepatica in galba truncatula its main intermediate host in western europe. in the present study two dna extraction techniques are compared and a new multiplex pcr is described. the chelex(®) dna extraction technique showed to be more appropriate than the classical phenol/chloroform/proteinase k based method because of the absence of toxic organic solvent, shorter duration and lower ...201021242033
immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of paramphistomum epiclitum.polypeptide profile of somatic antigen of paramphistomum epiclitum (psag) and gastrothylax crumenifer (gsag) was studied by sds-page. psag and gsag showed 14 and 19 polypeptides in the range of 14.9-95.5 and 13.7-129.6 kda with six common polypeptides of mol wt 16.8, 21.8, 23.7, 35.5, 43.4 and 70.8 kda. p. epiclitum experimentally infected sheep sera were used for identification of specific immuno-dominant peptide in the range of 37-40 kda against p. epiclitum by western blotting. hyperimmune se ...201021526036
tegumental surface changes in adult paramphistomum microbothrium (fischoeder 1901) following in vitro administration of artemether.the treatment of paramphistomiasis, a neglected tropical disease, has been carried out with different fasciolicidal compounds, all showing weak efficacy. therefore, the search for alternative paramphistomicidal drugs is warranted. in the present study, the in vitro effects of artemether on adult paramphistomum microbothrium were evaluated, for the first time, using scanning electron microscopy. after 24 h of incubation with 10 microg ml(-1) artemether, tegumental damage of both anterior and post ...201019691911
evaluation of medicinal plant extracts against blood-sucking parasites.the present study was based on assessments of the antiparasitic activities to determine the efficacies of acetone, chloroform, ethyl acetate, hexane, and methanol dried leaf, flower, and seed extracts of cassia auriculata l., rhinacanthus nasutus kurz., solanum torvum swartz, terminalia chebula retz., and vitex negundo linn. were tested against larvae of cattle tick rhipicephalus (boophilus) microplus canestrini, 1887 (acari: ixodidae), adult of haemaphysalis bispinosa neumann, 1897 (acarina: ix ...201020306205
laboratory determination of efficacy of indigenous plant extracts for parasites control.the present study was based on assessments of the antiparasitic activities to determine the efficacies of acetone, chloroform, ethyl acetate, hexane, and methanol dried leaf, flower, and seed extracts of achyranthes aspera l., anisomeles malabarica (l.) r. br., gloriosa superba l., psidium guajava l., ricinus communis l., and solanum trilobatum l. tested against the larvae of cattle tick rhipicephalus (boophilus) microplus (canestrini 1887) (acari: ixodidae), sheep internal parasite paramphistom ...200919308453
adulticidal and larvicidal efficacy of some medicinal plant extracts against tick, fluke and mosquitoes.the adulticidal and larvicidal effect of indigenous plant extracts were investigated against the adult cattle tick haemaphysalis bispinosa neumann, 1897 (acarina: ixodidae), sheep fluke paramphistomum cervi zeder, 1790 (digenea: paramphistomatidae), fourth instar larvae of malaria vector, anopheles subpictus grassi and japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae). the aim of this study was to evaluate the toxic effect of leaf hexane, chloroform, ethyl acetate, ...200919819626
the redial and cercarial production of a digenean in the snail host is lower when no cercarial shedding occurs.single- and double-miracidium exposures of galba truncatula with fasciola hepatica (two groups) or with paramphistomum daubneyi (two groups) were carried out under laboratory conditions to compare parasite production in cercaria-shedding snails (cs snails) with that found in snails without emission (ncs snails). free rediae and cercariae were thus counted in snails from both categories after their dissection at regular intervals (at 24 degrees c). in the four groups, the numbers of free rediae a ...200920092063
in vitro screening for anthelmintic and antitumour activity of ethnomedicinal plants from thailand.this study screened for anthelmintic and/or antitumour bioactive compounds from thai indigenous plants and evaluated effectiveness against three different worm species and two cancer cell lines.200919473794
paramphistomum daubneyi: the number of sporocysts developing in experimentally and naturally infected galba truncatula.experimental infections of galba truncatula with paramphistomum daubneyi were carried out to determine at day 50 (at 24 degrees c) the numbers of sporocysts, which grew in infected snails via the count of first- and second-generation rediae. in snails individually exposed to one, two, three, four, or five miracidia, the numbers of first-generation rediae increased from the one-miracidium group to the five-miracidium snails (from a mean of 6.7 to 26.1), while second-generation rediae decreased in ...200818470698
the hemoglobins of the trematodes fasciola hepatica and paramphistomum epiclitum: a molecular biological, physico-chemical, kinetic, and vaccination study.the trematode fasciola hepatica (fa.he.) is a common parasite of human and livestock. the hemoglobin (hb) of fa.he., a potential immunogen, was chosen for characterization in the search for an effective vaccine. characterization of trematode hbs show that they are intracellular single-domain globins with the following remarkable features: (1) fa.he. expresses two hb isoforms that differ at two amino acid sites (f1: 119y/123q; f2: 119f/123l). both isoforms are monoacetylated at their n-termini; ( ...200818621914
paramphistomum daubneyi: production dynamics and infectivity of metacercariae originating from snails dissected at regular intervals.experimental infections of galba truncatula with paramphistomum daubneyi were carried out at 24 degrees c to study the dynamics of larval development in snails dissected at regular intervals and to determine if metacercarial production might be improved. when the shell height of snails (4, 5, 6 or 7 mm) at exposure increased (experiment a), the total number of metacercariae was significantly higher in the 6- and 7-mm snails than in the other two groups, and the differentiation period was shorten ...200818328114
parasitic infections in dairy cattle around hanoi, northern vietnam.in northern vietnam, dairy cattle are mainly managed in small-scale farms, where animals are kept confined and feeding occurs by cut and carry methods. in the present study the occurrence of parasitic infections was examined in five provinces around hanoi. a total of 201 farms were visited, and 334 stool and 239 blood samples were collected from calves younger than 3 months, animals between 3 and 24 months and adult cows. furthermore, 254 milk samples were collected from lactating animals. copro ...200818328629
the epidemiology of paramphistomosis of sheep (ovis aries l.) in the north west temperate himalayan region of india.an epidemiological study with the objective to assess the prevalence of paramphistomosis in association with season, age, sex and breed was carried out in naturally infected sheep over a period of two years from february 2005 to january 2007. gastrointestinal tract (git) and faecal examination were conducted monthly to monitor the seasonal occurrence of paramphistomosis. 793 sheep were examined in the first year, out of which 7.06% were positive for paramphistomum infection. in the second year, ...200818357509
paramphistomum cervi: antigenic profile of adults as recognized by infected cattle sera.an antigenic profile of adult paramphistomum cervi was revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting using sera from cattle naturally infected with p. cervi, fasciola gigantica and strongylids. sds-page of whole worm extracts exhibited 26 distinct protein bands. immunoblotting analysis of these proteins showed five major antigenic bands which were recognized by serum of individual cattle naturally infected with p. cervi. these antigenic prot ...200817892871
gastrointestinal parasite egg excretion in young calves in periurban livestock production in mali.to acquire the information needed to improve parasite control in periurban cattle production in mali, repeated sampling of faeces of 694 calves kept around bamako was done in 2003/2004. the effects of season, age, breed, management type, parasite control and presence of sheep on egg and oocyst counts were determined. a bayesian model was used with a negative binomial distribution and herd and individual effects, to account for the clustering of calves in herds and the repeated sampling. intervie ...200817573084
risk periods of infection by calicophoron daubneyi (digenea:paramphistomidae) in cattle from oceanic climate areas.from march 2001 to february 2004, 724 faecal samples of cattle under field conditions were collected in 121 farms from lugo (nw spain), an area with oceanic climate. the risk periods of infection by paramphistomum trematoda in cattle was assessed by analyzing the prevalence of paramphistomosis, egg-output and climatic data. a significantly higher percentage of cattle passing trematode eggs by faeces was recorded firstly in september and secondly in march. the greatest numbers of eggs were achiev ...200717323101
prevalence and intensity of infections in the lymnaeid snail omphiscola glabra experimentally infected with fasciola hepatica, fascioloides magna and paramphistomum daubneyi.single and double infections of juvenile omphiscola glabra (gastropoda: lymnaeidae) with paramphistomum daubneyi and/or fasciola hepatica were carried out to determine the redial burden and cercarial production in snails dissected at day 60 or at day 75 post-exposure (p.e.) in the laboratory at 20 degrees c. the results were compared with those obtained with single-miracidium infections by fascioloides magna. compared to f. hepatica, low values were noted at day 75 p.e. for the prevalence of sna ...200717381861
paramphistomum spp: improved artificial excystment and in vitro culture of immature and adult stages.the aims of the present study were to establish an adequate excystment method for metacercariae of paramphistomum spp. and to compare the in vitro survival of paramphistomids in three different culture media. in a preliminary study, 31 different assays were performed solely with the purpose of obtaining the best percentage of metacercariae excystment. once the best excystment method was selected, 21 further assays using 100 metacercariae/assay were carried out to determine the average percentage ...200717768639
prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern ethiopia.a 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (gi) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern ethiopia. during may 2003 to april 2005, viscera including liver, lungs and gi tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of haramaya, harar, dire dawa and jijiga in eastern ethiopia. all animals were raised in the farming areas ...200717969715
parasites of the digestive tract in beef cattle imported from france to italy.beef cattle heads (195 heads, 6 batches) imported for fattening from france to italy were examined. coprological qualitative and quantitative tests were performed, and the results analysed in relation to sex, breed, age, date of arrival, geographical origin (french department in which the animal was bred), and gathering centre (french department in which the animal was grouped with the others before travelling to italy). the following parasites were identified: eimeriidae (overall prevalence 60. ...200718412052
multivariate spatially-structured variability of ovine helminth infections.a cross-sectional survey was carried out on 2004-2005 in the campania region, southern italy, to study the multivariate geographical distribution of four different sheep helminths, i.e. fasciola hepatica (liver fluke), calicophoron (paramphistomum) daubneyi (rumen fluke), dicrocoelium dendriticum (lancet fluke), and the gastrointestinal strongyle haemonchus contortus. a series of multivariate bayesian hierarchical models based on square root transformation of faecal egg counts were performed. th ...200718686259
ultrastructure of spermiogenesis and the spermatozoon of paramphistomum microbothrium [fischoeder 1901] (digenea: paramphistomidae), a parasite of bos taurus in senegal.the ultrastructural study of spermiogenesis and the spermatozoon of paramphistomum microbothrium gave evidence of some characteristics of this digenean. the intercentriolar body located between two striated roots and two centrioles exhibits a symmetric plane. the two external bands of this intercentriolar body are made up of a line of granules. the striated roots are about 3.7 microm long. during spermiogenesis, a flagellar rotation of 90 degrees is described. the spermatozoon is characterized i ...200717401579
parasite development and visceral pathology in galba truncatula co-infected with fasciola hepatica and paramphistomum daubneyi.histological investigations in galba truncatula naturally or experimentally co-infected with fasciola hepatica and paramphistomum daubneyi were carried out to study parasite development and the responses of the digestive gland and kidney of snails, as larval forms of these digeneans often use these two sites for their growth within the snail's body. the number of live rediae per snail ranged from 2.4 to 4.2 for the dominating parasite (it developed in the digestive gland) and was less than 2.0 f ...200717875230
optimization of metacercarial production for three digenean species by the use of petri dishes for raising lettuce-fed galba truncatula.experimental infections of galba truncatula with fasciola hepatica, fascioloides magna, or paramphistomum daubneyi were carried out at 20 degrees c to determine if the use of 14-cm petri dishes for breeding lettuce-fed snails enhanced the characteristics of snail infections. compared to infected snails raised in boxes up to day 30 post-exposure and later in individual 35-mm dishes, the survival of g. truncatula kept in 14-cm dishes and the shell height of cercariae-shedding snails during the fir ...200717061111
ultrastructure of spermiogenesis and the spermatozoon of paramphistomum microbothrium (fischoeder 1901; digenea, paramphistomidae), a parasite of bos taurus in senegal.the ultrastructural study of spermiogenesis and the spermatozoon of paramphistomum microbothrium gave evidence of some characteristics of this digenean. the intercentriolar body located between two striated roots and two centrioles exhibits a symmetric plane. the two external bands of this intercentriolar body are made up of a line of granules. the striated roots are about 3.7-microm long. during spermiogenesis, a flagellar rotation of 90 degrees is described. the spermatozoon is characterized i ...200717323137
reliability of coprological diagnosis of paramphistomum sp. infection in cows.a modified macmaster method was tested to check its reliability for the diagnosis of bovine paramphistomosis in france. a total number of 148 fecal samples from cows examined post-mortem were analysed. coprological results were in accordance with necropsic examinations. bayesian techniques (markov chain monte carlo) were used to estimate the diagnostic parameters of each of these tests. two scenarios were envisaged: one assuming a sensitivity of the necropsic examination equal to 1 and one assum ...200717391854
high annealing temperature-random amplified polymorphic dna (hat-rapd) analysis of three paramphistome flukes from thailand.the rumen flukes of 37 cows (bos indicus) from chiang mai and lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). three species were found: paramphistomum epiclitum, orthocoelium streptocoelium, and fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. genomic dna was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic dna (hat-rapd) technique. ...200716889772
prepatent detection of fasciola gigantica infection in bovine calves using metacercarial antigen.metacercarial antigen of fasciola gigantica was evaluated for early immunodiagnosis of experimental bovine fasciolosis using elisa and western blot. in elisa, the experimental f. gigantica infection was detected as early as 2 weeks post-infection (wpi). the gradual increasing trend of antibody level was observed from 2 to 7 wpi, followed by a plateau, which was maintained up to 14 wpi. in western blot, sera from experimentally infected calves recognized one distinct polypeptide of 21 kda in frac ...200616999031
cathepsin l cysteine proteinase in the diagnosis of bovine fasciola gigantica infection.cathepsin l cysteine proteinase from fasciola gigantica was evaluated for its potential in the early prepatent detection of this helminth infection in bovine calves. five cross-bred bovine calves were experimentally infected with 400 metacercariae/calf and evaluated for anti-cathepsin l antibody response. f. gigantica infection in these calves could be detected 4 weeks post-infection using an elisa, dipstick elisa and western blotting with 100% sensitivity. the antigen was also used to detect f. ...200616300897
immuno-diagnosis of bubaline fasciolosis with fasciola gigantica cathepsin-l and recombinant cathepsin l 1-d proteases.fasciola gigantica cathepsin-l cysteine proteinase and recombinant cathepsin l 1-d were assessed for their potential in the immuno-diagnosis of f. gigantica infection in buffaloes. a diagnostic elisa, based on these two antigens, was developed to detect antibodies against f. gigantica in water buffaloes. sensitivity of the elisa was assessed using sera from buffaloes experimentally or naturally infected with f. gigantica from f. gigantica endemic areas and its specificity by probing the sera of ...200616647680
natural infection by paramphistomoidea stiles and goldberger, 1910 trematodes in wild marsh deer (blastocerus dichotomus illiger, 1815) from sérgio mottas's hydroelectric power station flooding area.studies on helminthfauna of marsh deer blastocerus dichotomus illiger, 1815 are rare, although helminthic diseases are an important cause of mortality in these animals. fifteen male and female adult marsh deer from sergio motta's hydroelectric power station flooding area at paraná river which died during the capture and quarantine procedures, between 1998 and 1999, were necropsied. three trematodes species, paramphistomum cervi, balanorchis anastrofus and zygocotyle lunatum, all belonging to sup ...200617196114
dna quantities and qualities from various stages of some trematodes using optical and hat-rapd methods.the aim of this experiment was to minimize dna quantity and quality for detection by optical (spectrophotometer at 260 nm and 280 nm) and hat-rapd methods. total dna from different stages, adult, metacercaria and eggs of 6 trematode species were isolated for analysis. in this experiment, the adult trematodes were classified into 3 groups by size: small, haplorchis taichui and stellantchasmus falcatus; medium, opisthorchis viverrini and ganeo tigrinus; and large, paramphistomum epiclitum and fisc ...200617547055
multiple active site conformers in the carbon monoxide complexes of trematode hemoglobins.sequence alignment of hemoglobins of the trematodes paramphistomum epiclitum and gastrothylax crumenifer with myoglobin suggests the presence of an unusual active site structure in which two tyrosine residues occupy the e7 and b10 helical positions. in the crystal structure of p. epiclitum hemoglobin, such an e7-b10 tyrosine pair at the putative helical positions has been observed, although the e7 tyr is displaced toward cd region of the polypeptide. resonance raman data on both p. epiclitum and ...200616481317
some parasitic flukes infecting farm animals in al-santa center, gharbia governorate, egypt.a total of 213 farm animals in the vicinity of al-santa center (73 cattle, 90 buffaloes and 50 sheep) were coprologic examined for natural infection with trematod-parasites. the results showed that cattle were infected with fasciola sp (21.8%) and paramphistomum sp. (7.3%), buffaloes were infected with fasciola sp. (17.7%) and paramphistomum sp. (10%), while sheep were infected with fasciola sp. (30%), d. dendriticum (5%) and paramphistomum sp. (4%). the three animal species were treated for par ...200616605115
survey of helminths in adult sheep in heilongjiang province, people's republic of china.the prevalence of helminths in adult sheep was investigated in heilongjiang province, people's republic of china between january 1999 and september 2003. a total of 326 adult sheep representing local breeds (xingjiang fine wool sheep, dongbei fine wool sheep) as well as introduced breeds (merino and charollais) from representative geographical locations in heilongjiang province were slaughtered and examined for the presence of helminths. the worms were examined, counted and identified to species ...200616713098
characterization of specific and cross-reacting antigens of fasciola gigantica by immunoblotting.somatic antigens of f. gigantica, g. explanatum, s. spindale and hydatid cyst ingredients were analysed to identify the cross-reactive antigens among them using western blot technique. when probed with f. gigantica infected cattle sera, the immunodominant 156 kda and 28 kda proteins of f. gigantica was found common amongst the antigens prepared from hydatid cysts ingredients like germinal layer, fertile and sterile, hydatid fluid, fertile and sterile, while another protein of 34 kda was shared b ...200515952043
27 kda fasciola gigantica glycoprotein for the diagnosis of prepatent fasciolosis in cattle.an indirect enzyme-linked immunosorbent assay (elisa) using 27 kda glycoprotein of fasciola gigantica has been evaluated for its potential use in the diagnosis of bovine fasciolosis. following experimental infection of rabbits, f. gigantica infection-induced antibodies were isolated and later used as ligands in affinity chromatography for isolation of infection-induced antibody-specific proteins. among the five infection-specific proteins isolated, a glycoprotein of 27 kda was later isolated by ...200515730136
comparative diagnostic potentiality of elisa and dot-elisa in prepatent diagnosis of experimental fasciola gigantica infection in cattle.a glycoprotein (27 kda) was isolated from crude somatic antigen of fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect elisa and in dot-elisa formats. although, anti-27 kda antibodies could be detected after 3 weeks post infection (wpi) by dot - elisa which was one week later than indirect elisa. the test, dot-elisa, was more convenient in field application. by the test (dot-elisa) the infection could be equall ...200515991579
fasciola hepatica: epidemiological surveillance of natural watercress beds in central france.a total of 59 natural watercress beds in the limousin region (central france) was surveyed over a 15-year period (1990-2004) to detect the contamination of watercress by the metacercariae of fasciola hepatica and to determine the presence of larval forms in the two species of lymnaeids which live in these waterholes in june and july. the number of beds contaminated with f. hepatica metacercariae varied over the years, and the burden of the larvae on plants was low: a mean of 2.6-6.3 per bed. the ...200515682341
fasciola hepatica and paramphistomum daubneyi: field observations on the transport and outcome of floating metacercariae in running water.experimental investigations in eight open drainage ditches and furrows from central france were carried out to analyse the dispersal of floating metacercariae of two digenean species by running water and to determine the outcome of larvae which settled on nasturtium officinale (watercress). the frequencies of larvae found after their transport by water ranged from 33% to 49.7%, thus indicating that more than half of the metacercariae used in this experiment had fallen to the bottom of the water ...200415153290
fasciola hepatica: the developmental patterns of redial generations in naturally infected galba truncatula.a retrospective study on 1,211 snails naturally infected with fasciola hepatica in central france was carried out to determine the numbers of full-grown sporocysts from which infections derived, to specify the developmental patterns of redial generations (normal or abnormal) and to count live and free rediae. in the department of haute vienne (siliceous subsoils), most snails showed single-sporocyst infections, with normal (46.3%) or abnormal (33.9%) development of redial generations. two-sporoc ...200415338285
fasciola hepatica and paramphistomum daubneyi: vertical distribution of metacercariae on plants under natural conditions.four experiments on the metacercariae of fasciola hepatica and paramphistomum daubneyi were carried out under natural conditions in order to study their vertical location on submerged plants and to determine whether simultaneous cercarial shedding of both digenea causes changes in the distributions of the metacercariae. these experiments were performed in experimental boxes, each containing six tufts of rushes. most metacercariae (73.0%) of f. hepatica were found along the walls of boxes, while ...200415338293
seasonal trends of paramphistomum cervi in tabasco, mexico.thirty rumens were examined every month from may 1989 to april 1992 to establish seasonal trends of infection and their relationship to the climatic factors caused by paramphistomum cervi in cattle. the cattle were chosen from three municipalities of the sierra region in tabasco: jalapa, tacotalpa and teapa. p. cervi was found throughout the year with prevalence between 3.33 and 96.67%, and with a yearly average of 39.10%. the major period of infection was shown in jalapa. however, the maximum m ...200314559164
natural infections of omphiscola glabra (lymnaeidae) with fasciola hepatica in central france.as larval forms of fasciola hepatica have periodically been detected in omphiscola glabraafter their collection from watercress beds or from meadows since 1995, field investigations in 37 populations of o. glabra were carried from 1996 to 2002. this was done in order to determine the changes in prevalences and intensities of these natural infections with f. hepatica in relation to the type of snail habitat and the year of snail collection. snails infected with f. hepatica were found in all sampl ...200314564511
1h nmr study of the molecular structure and magnetic properties of the active site for the cyanomet complex of o2-avid hemoglobin from the trematode paramphistomum epiclitum.the solution molecular and electronic structures of the active site in the extremely o2-avid hemoglobin from the trematode paramphistomum epiclitum have been investigated by 1h nmr on the cyanomet form in order to elucidate the distal hydrogen-bonding to a ligated h-bond acceptor ligand. comparison of the strengths of dipolar interactions in solution with the alternate crystal structures of methemoglobin establish that the solution structure of wild-type hb more closely resembles the crystal str ...200312823541
molecular cloning and immunolocalization of the 17 kda myoglobin of clonorchis sinensis.we purified the 17 kda protein abundant in clonorchis sinensis crude extracts. the n-terminal amino acid sequence of this protein was determined and an oligonucleotide probe synthesized. using this probe, the cdna encoding the protein was cloned and sequenced from the c. sinensis cdna library. it was found to consist of a total of 150 amino acids and to have 41% conserved homology with the myoglobin of the trematodes paramphistomum epiclitum and isoparorchis hypselobagri. the gene product over-e ...200312720088
unusual snail species involved in the transmission of fasciola hepatica in watercress beds in central france.four freshwater pulmonate species (lymnaea ovata, l. stagnalis, physa acuta, planorbis leucostoma) were living in several watercress beds known for their relationships with human cases of fasciolosis, whereas l. truncatula was never found. the aims of these studies were to determine the prevalence of natural infections with fasciola hepatica in snails and to verify if these species might ensure the full larval development of this trematode (with cercarial shedding) when they were experimentally ...200212116856
fasciola hepatica and paramphistomum daubneyi: changes in prevalences of natural infections in cattle and in lymnaea truncatula from central france over the past 12 years.a retrospective study was carried out over a 10- to 12-year period to analyse the changes in prevalences of natural fasciolosis and paramphistomosis among cattle and snails in central france, and to determine the causes which had induced these changes. the prevalences of natural fasciolosis in cattle increased from 1990 to 1993 (13.6% to 25.2%) and diminished afterwards up to 1999 (at 12.6%). those of natural paramphistomosis showed a progressive increase between 1990 and 1999 (from 5.2 to 44.7% ...200212387481
the influence of different food sources on cercarial production in lymnaea truncatula experimentally infected with digenea.experimental infections of lymnaea truncatula with a digenean species (fasciola gigantica, f. hepatica, or paramphistomum daubneyi) were performed under laboratory conditions to study the effect of four sources of food (microalgae, romaine lettuce, wheat germs, or modified boray diet) on cercarial production. the mean number of f. hepatica, f. gigantica, or p. daubneyi cercariae was significantly greater when snail food was microalgae or boray diet. the lowest cost prices for 100 metacercariae o ...200211873822
production and characterization of a monoclonal antibody against recombinant glutathione s-transferase (gst) of fasciola gigantica.a monoclonal antibody (moab) against a recombinant glutathione s-transferase (rgst) of f. gigantica was produced in balb/c mice. reactivity and specificity of this monoclonal antibody was assessed by elisa and immunoblotting. six stable clones, namely 3a3, 3b2, 3c6, 4a6, 4b1 and 4d6 were obtained, all these moab reacted with rgst and native gst at a molecular weight of 28 kda and found to be igg1, kappa-light chain isotypes. these moab cross-reacted with schistosoma mansoni and schistosoma japon ...200212744627
structural plasticity in the eight-helix fold of a trematode haemoglobin.the three-dimensional structure of recombinant haemoglobin from the trematode paramphistomum epiclitum, displaying the highest oxygen affinity so far observed for (non)vertebrate haemoglobins, has previously been determined at 1.17 a resolution (orthorhombic space group p2(1)2(1)2(1)). in the present communication, the three-dimensional structure of wild-type p. epiclitum haemoglobin is reported at 1.85 a resolution in a monoclinic crystal form (r factor = 16.1%, r(free) = 22.0%). comparison of ...200211914507
production and characterization of a monoclonal antibody against recombinant fatty acid binding protein of fasciola gigantica.in fasciola parasites fatty acid binding proteins (fabps) are the carrier proteins that help in the uptake of fatty acids from the hosts' fluids. attempts have been made to utilize both native and recombinant fabp (rfabp) for immunodiagnosis and vaccine development for fasciolosis. in this study, we have produced a number of monoclonal antibodies (moabs) against rfabp of fasciola gigantica. these moabs were initially screened against rfabp by elisa and then tested for their specificities by immu ...200211900926
the endoparasites of sheep and goats, and shepherd in north sinai governorate, egypt.examination of sheep and goats in four areas in north sinai revealed an overall infection of 12.70% with fasciola species, 11.8% with paramphistomum cerve, 12.80% with moniezia expansa, 47.11% with trichostrongylus colubriformis, 4.59% trichuris ovis and 44.85% coccidia. the highly infected sheep and goats were diagnosed in el hassanah center (90.49%), followed by al arish city (87.31%), then rafah city (74.39%), and lastly bir al abd (54.71%). the elevation of the overall percent of infection i ...200212049248
production and characterization of a monoclonal antibody against 28.5 kda tegument antigen of fasciola gigantica.a monoclonal antibody (moab) against the 28.5 kda tegumental antigen of fasciola gigantica was produced by the hybridoma technique using spleen cells from balb/c mice immunized with the tegumental extract from adult f. gigantica. this moab was found to be of the isotype igg(1), kappa-light chain, and shown by immunoblotting to specifically react with the 28.5 kda antigen present in the tegument, excretion-secretion material of the adult, whole-body extracts of newly excysted juveniles, 5-week-ol ...200212387905
very high resolution structure of a trematode hemoglobin displaying a tyrb10-tyre7 heme distal residue pair and high oxygen affinity.monomeric hemoglobin from the trematode paramphistomum epiclitum displays very high oxygen affinity (p(50)<0.001 mm hg) and an unusual heme distal site containing tyrosyl residues at the b10 and e7 positions. the crystal structure of aquo-met p. epiclitum hemoglobin, solved at 1.17 a resolution via multiwavelength anomalous dispersion techniques (r-factor=0.121), shows that the heme distal site pocket residue tyrb10 is engaged in hydrogen bonding to the iron-bound ligand. by contrast, residue ty ...200111399085
metacercarial aggregation in digenea (fasciola hepatica and paramphistomum daubneyi): environmental or species determinism?metacercarial aggregation of fasciola hepatica and paramphistomum daubneyi was studied under experimental conditions to determine if the formation of these aggregates was influenced by environmental factors, or it was a characteristic of trematode species. this process was studied using the confinement of infected snails on the bottom of petri dishes (diameter, 14 cm) for 3 days. the formation of metacercarial aggregates of f. hepatica was not significantly modified by environmental factors such ...200111818045
relationship between helminth species diversity, intensity of infection and breeding management in dairy goats.helminth infection, species diversity (proportion of each species in the community), species number, intensity of infection and anthelmintic resistance were investigated in 16 dairy-goat farms of south-western france. the aim of the study was to estimate which breeding management factors may affect diversity and intensity of infection. anthelmintic resistance was investigated because this factor may modify composition of nematode communities. farm history and breeding management were recorded by ...200011078947
cercarial productivity of redial generations in single-miracidium infections of lymnaea truncatula with paramphistomum daubneyi or fasciola hepatica.single-miracidium infections of lymnaea truncatula with paramphistomum daubneyi or with fasciola hepatica were carried out under laboratory conditions to count free rediae, their germinal embryos, and to determine the cercarial productivity of each redial generation. in snails infected by p. daubneyi, the cercariae were produced by the first (8.7 cercariae per redia) and second (8.9 per redia) generations. at day 63 post-exposure, they corresponded, respectively, to 53.9% and 46.1% of cercariae ...200010831047
the epidemiology of nematode and fluke infections in cattle in the red river delta in vietnam.over a period of 13 months, faecal samples were collected monthly from approximately 45 cattle over 3 months of age. additionally, 74 calves of 1-2 months were sampled to determine the presence of toxocara vitulorum eggs. individual egg counts and infective strongyle larvae from pooled faecal samples were examined. post-mortem worm counts were carried out on six groups of tracer calves (n=12) that had been kept for 4 weeks on pasture in and around the village studied. the following helminths wer ...200011035232
[planorbinae snails--obligate intermediate hosts of paramphistomatid trematodes in the central polesye of ukraine].the data on species composition, ecology and distribution of snails of the subfamily planorbinae (tribes planorbini, segmentinini) in water basis of tsentral'noye polesye are given. in 13 species belonging to the genera planorbis, anisius, choanomphalus, segmentina, hippeutis, the parthenitae and larvae of paramphistomum ichikawai jamaguti has been recorded. infection rate varies from 0.5 to 18.5%.200011060894
a field study of natural infections in three freshwater snails with fasciola hepatica and/or paramphistomum daubneyi in central france.natural infections of three freshwater snails with fasciola hepatica and/or paramphistomum daubneyi were studied during two periods in 1996 and 1997 (june-july and september-october) on 18 farms located in the departments of vienne and haute vienne (central france), and known for low prevalences of f. hepatica infections in ruminants. a total of 1573 lymnaea glabra and 1421 l. truncatula 6 mm high or more were collected in the meadows of 13 farms and dissected under laboratory conditions. snails ...200010953217
prevalence of paramphistomum daubneyi infection in cattle in central france.investigations at the slaughterhouse of limoges (central france) were performed in 1994-1996 to determine the prevalence of paramphistomum daubneyi infection in cattle. in 1994/1995, higher prevalences of p. daubneyi were recorded in may, october, and january. in 1996, smaller variations were recorded, and prevalence in april and may was higher. the prevalence of p. daubneyi in summer was lower. breed and age of cattle were not significantly associated to p. daubneyi infection, but females were ...200010622604
modulation of the oxygen affinity of cobalt-porphyrin by globin.we have combined two extreme effects which influence the oxygen affinity to obtain a cobalt-based oxygen carrier with an affinity similar to that of human adult hemoglobin (hba). the goal was to obtain an oxygen transporter with a lower oxidation rate. exchange of the heme group (fe-protoporphyrin ix) in hb with a cobalt-porphyrin leads to a reduction in oxygen affinity by over a factor of 10, an oxygen affinity too low for use as a blood substitute. at the other extreme, certain globin sequence ...200010788615
caprine paramphistomum daubneyi (trematoda) infection in europe. 200010883858
functional differentiation in trematode hemoglobin isoforms.the hbs and the major electrophoretic hb components (isohbs) were isolated from three species of the trematodes, explanatum explanatum (ee), gastrothylax crumenifer (gc) and paramphistomum epiclitum (pe), that parasitise the common indian water buffalo bubalus bubalis. the hbs are monomeric and resemble the so-called nonfunctional mutant hemoglobins that have tyr at b10 or e7 positions (replacing leu and the his residues, respectively). however, they are capable of binding with o2 and co. o2 equ ...199910103000
influence of low temperatures on the cercarial shedding of paramphistomum daubneyi from the snail lymnaea truncatula.lymnaeids in nature are subjected to temperature fluctuations that may have an influence on the shedding of cercariae. thus, experimental infections of lymnaea truncatula by paramphistomum daubneyi were performed to determine whether a sudden fall in temperature--daily dipping of infected snails into spring water at 14-15 degrees c, or at 6-8 degrees c--followed by its increase at 20 degrees c in the subsequent hour, had an influence on the characteristics of snail infection and cercarial produc ...199910229944
standardization of a dot immunoperoxidase assay for field diagnosis of fasciola hepatica infected cattle.a dot immunoperoxidase assay was developed for the detection of bovine specific antibodies to fasciola hepatica. the standardization of the assay was done with 298 bovine sera, from seven different groups, previously studied by the reference enzyme-linked immunosorbent assay (elisa). f. hepatica surface antigens purified by negative affinity against epitopes shared with paramphistomum spp. and echinococcus granulosus were used. starting with sensitized and blocked nitrocellulose strips, the dot ...199910488728
infection of lymnaea truncatula and lymnaea glabra by fasciola hepatica and paramphistomum daubneyi in farms of central france.natural infection of lymnaea glabra and lymnaea truncatula by fasciola hepatica and/or paramphistomum daubneyi was studied at two periods of risk (june-july and september-october) in 11 french farms known for their high prevalences of f. hepatica infection in ruminants. a total of 1,778 l. truncatula and 2,396 l. glabra measuring 6 mm or more in height were collected to determine the prevalence of natural infection with f. hepatica and p. daubneyi. the role of four factors, i.e. season, snail co ...199910081118
paramphistomum daubneyi and fasciola hepatica: influence of temperature changes on the shedding of cercariae from dually infected lymnaea truncatula.dual infections of lymnaea truncatula with paramphistomum daubneyi and fasciola hepatica were performed to determine whether temperature changes in snails (daily water change with spring water at 6 degrees-8 degrees c, which subsequently increased to room temperature at 20 degrees c) would influence snail infection and the production of cercariae by both trematodes. at day 30 post-exposure the surviving snails were individually placed in petri dishes to constitute two groups. snails from the fir ...199910431746
trematode hemoglobins show exceptionally high oxygen affinity.ligand binding studies were made with hemoglobin (hb) isolated from trematode species gastrothylax crumenifer (gc), paramphistomum epiclitum (pe), explanatum explanatum (ee), parasitic worms of water buffalo bubalus bubalis, and isoparorchis hypselobagri (ih) parasitic in the catfish wallago attu. the kinetics of oxygen and carbon monoxide binding show very fast association rates. whereas oxygen can be displaced on a millisecond time scale from human hb at 25 degrees c, the dissociation of oxyge ...19989675199
unusual transmission of the liver fluke, fasciola hepatica, by lymnaea glabra or planorbis leucostoma in france.cases of fasciolosis in ruminants have been recorded in several french farms in the absence of lymnaea truncatula, which is considered the only snail intermediate host in western europe. these farms harbored other species of freshwater snails in large numbers (lymnaea glabra, physa acuta, or planorbis leucostoma) and, in many cases, had cattle or sheep infected by another trematode (paramphistomum daubneyi). these other freshwater snails may serve as intermediate hosts for f. hepatica due to a c ...19989920323
comparison of three elisa tests for seroepidemiology of bovine fascioliosis.the aim of the present study was to compare the sensitivity, specificity and usefulness of the dig-elisa, dot-elisa and indirect elisa tests for determining the seroprevalence of fasciolosis in cattle under tropical conditions in mexico. to standardize the tests, positive and negative sera to f. hepatica from 88 holstein freisian adult cows located in an enzootic area of fascioliosis and 88 crossbred adult cattle from a fluke-free area were used. for the epidemiological study, 85 crossbred cattl ...19989763313
in vitro anthelmintic activity of root-tuber extract of flemingia vestita, an indigenous plant in shillong, india.the in vitro activity of root-tuber-peel extract of flemingia vestita, an indigenous plant consumed by the natives in northeast india, was tested against helminth parasites. live parasites (nematode: ascaris suum from pigs, a. lumbricoides from humans, ascaridia galli and heterakis gallinarum from domestic fowl; cestode: raillietina echinobothrida from domestic fowl; trematode: paramphistomum sp. from cattle) were collected in 0.9 % physiological buffered saline (pbs) and maintained at 37 +/- 1 ...19979197399
development and characterization of monoclonal antibodies against excretory-secretory antigens of fasciola gigantica.monoclonal antibodies (mabs) directed against fasciola gigantica excretory-secretory (es) antigens were developed from balb/c mice. four were selected for further study, from the panel of hybridomas. the antigen specificities of these mabs were characterized and localized by enzyme-linked immunoeletrotransfer blot (eitb) and immunoperoxidase technique. the target epitopes of these mabs are 66 kda protein (mab 2d10), 66 and 27-26 kda proteins (mabs 5d10 and 4f5) and 27-26 kda protein (mab 2d9). m ...19979656363
the prevalence of fasciola hepatica in its snail intermediate host determined by dna probe assay.accurate snail intermediate host infection prevalence data have the potential to be extremely useful in determining seasonal transmission dynamics of fasciola hepatica. because the microscopic techniques currently used lack the sensitivity and specificity necessary to obtain meaningful infection prevalence data, we developed a highly accurate and efficient dna probe assay. the assay has a sensitivity of 100%, a specificity of > 99%, easily detects a single miracidia and does not cross-hybridize ...19979467746
paramphistomum daubneyi: the development of redial generations in the snail lymnaea truncatula.rediae of paramphistomum daubneyi were counted and measured in lymnaea truncatula to elucidate the variability in the numbers of free rediae and cercariae occurring between naturally infected snails and experimental single-miracidium infections. experiments were performed using one miracidium per snail and snail raising was carried out at 20 degrees c. two redial generations succeeded each other in the snail until day 49. a mean of 8-10 rediae differentiated in the sporocyst at days 7 and 14; th ...19979000237
trematode myoglobins, functional molecules with a distal tyrosine.the myoglobins of two trematodes, paramphistomum epiclitum and isoparorchis hypselobagri, were isolated to homogeneity. the native molecules are monomeric with mr 16,000-17,000 and pi 6.5-7.5. in each species, at least four different globin isoforms occur. primary structure was determined at the protein level. the globin chains contain 147 amino acid residues. although major determinants of the globin fold are conserved, characteristic substitutions are present. a tyr residue occurs at the helic ...19979006947
solution of 1h nmr structure of the heme cavity in the oxygen-avid myoglobin from the trematode paramphistomum epiclitum.a two-dimensional 1h nmr study has been carried out on the heme cavity of the extreme oxygen-avid and autoxidation-resistant oxy-myoglobin complex from the trematode paramphistomum epiclitum, and the residues were identified which potentially provide hydrogen bond stabilization for the bound oxygen. complete assignment of the heme core resonances allows the identification of 10 key heme pocket residues, 4 phe, 4 tyr, and 2 upfield ring current aliphatic side chains. based solely on the conserved ...19979006948
faecal examination of fasciola eggs fixed with formalin solution using the beads technique.faecal examination for fasciola eggs which were preserved in 5% and 10% formalin and taf solutions for 6 years was carried out using the beads technique. the morphological characters and the distinctive yellowish color of fasciola eggs were retained and appeared similar to those of fresh eggs. the mean egg recovery rate of eggs in taf solution was significantly lower than the others (p < 0.01). the egg recovery rates from 5% and 10% formalin samples were 57.2% and 60.8%, respectively, and these ...19969017874
prevalence and seasonal changes in the gastro-intestinal helminths of nigerian goats.a total of 120 gastro-intestinal tracts and 960 faecal samples were examined to assess the prevalence and seasonal changes in the gastro-intestinal helminth parasites of red sokoto (maradi) goats slaughtered at ibadan between may 1991 and april 1992. egg types of strongyles, strongyloides, trichuris, skrjabinema, dicrocoelium and moniezia were encountered in 93%, 83%, 44%, 0.9%, 2.3% and 31% of the faecal samples respectively. however, only strongyle, strongyloides and trichuris eggs occurred in ...19968960229
paramphistomum daubneyi and fasciola hepatica: the effect of dual infection on prevalence and cercarial shedding in preadult lymnaea glabra.preadult lymnaea glabra measuring 4-6 mm in height were each exposed to 1 paramphistomum daubneyi miracidium before being exposed to 1 miracidium of fasciola hepatica. total prevalence of infection in the snail groups from 3 different populations ranged from 33% to 39%. in each group, snails harboring larval forms of p. daubneyi, f. hepatica, or both, were noted. if the results from the 3 snail populations are pooled, the total prevalence of snail infection was 13.6% in snails harboring only f. ...19968973417
paramphistomum daubneyi and fasciola hepatica: the redial burden and cercarial shedding in lymnaea truncatula subjected to successive unimiracidial cross-exposures.the development of redial burden and cercarial shedding were studied in two groups of lymnaea truncatula subjected to successive cross-exposures to one miracidium of paramphistomum daubneyi and one of fasciola hepatica per snail, or vice versa. the results were compared with those obtained in controls subjected to two unimiracidial exposures to the same trematode species. the infection rate was 61% in the group cross-exposed to p. daubneyi/f. hepatica and 37% in that cross-exposed to f. hepatica ...19968875570
detection of circulating fasciola gigantica antigen in experimental and natural infections of sheep with fasciolosis.detection of circulating fasciola gigantica antigen was performed in sera of sheep with experimental and natural f. gigantica infections using the direct enzyme-linked immunosorbent assay method. sera from sheep with monoinfections of schistosoma bovis, dicrocoelium hospes and paramphistomum microbothrium were included in the assay to ascertain specificity. circulating f. gigantica antigen (cfa) was detected as early as 1 week after infection in the experimentally infected sheep. no detectable c ...19968916398
antibody and circulating antigen profiles before and after chemotherapy in goats infected with fasciola gigantica.the profiles of antibody response and circulating antigen levels in goats infected with fasciola gigantica were studied by enzyme-linked immunoelectrotransfer blot (eitb) and enzyme-linked immunosorbent assay (elisa). in the antibody assay, sera from goats experimentally infected with f. gigantica were reacted with whole worm antigen of the worm before and after chemotherapy with oxyclozanide. in elisa, there was a significant increase in antibody level 2 weeks after infection. after chemotherap ...19969017880
structural and functional comparison of trematode haemoglobins.haemoglobins from three different species of trematodes (gastrothylax crumenifer, paramphistomum epiclitum and isoparorchis hypselobagri) and their respective hosts were digested with papain. peptides so produced were separated on 10-15% sds-pagge. the probability of coincidence of the peptides was analysed statistically. oxygen affinity curves of trematode haemoglobins were found to be a rectangular hyperbolic. the hill coefficient values of all the trematode haemoglobins were found to be 1. th ...19957622793
[cryptosporidia and other endoparasites in heifers imported into the czech republic].totally 887 heifers of holstein-friesian breed mostly in late pregnancy imported to the czech republic from france (597), germany (89), denmark (181) and holland (20) were examined coprologically from september 1993 to march 1995 in the parasitological laboratory of the national veterinary institute (nvi). prague. feces were sampled individually, rectally, always on days 1-3 following importation from heifers housed in particular quarantine sheds. in compliance with presently valid veterinary re ...19958659084
time-course analysis of antibody response by eitb and elisa before and after chemotherapy in sheep infected with fasciola gigantica.the time-course analysis of the antibody response to fascicola gigantica infection in sheep was studied by enzyme-linked immunosorbent assay (elisa) and enzyme-linked immunoelectrotransfer blot (eitb). sera from sheep experimentally infected with f. gigantica were reacted with excretory-secretory antigens of the worm before and after chemotherapy with oxyclozanide. in elisa, there was a significant increase in anti-fasciola antibody by 2 weeks after infection and there was a sharp decrease in an ...19957571329
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