Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| replacing alfalfa silage with tannin-containing birdsfoot trefoil silage in total mixed rations for lactating dairy cows. | two lactation trials were conducted comparing the feeding value of silages made from birdsfoot trefoil (bft, lotus corniculatus l.) that had been selected for low (bftl), medium (bftm), and high (bfth) levels of condensed tannins (ct) to an alfalfa silage (as) when fed as the principal forage in total mixed rations. diets also included corn silage, high-moisture shelled corn, soybean meal, soy hulls, and supplemental fat. in trial 1, 32 lactating holstein cows were blocked by days in milk, assig ... | 2017 | 28259401 |
| chronic exposure to a neonicotinoid pesticide alters the interactions between bumblebees and wild plants. | insect pollinators are essential for both the production of a large proportion of world crops and the health of natural ecosystems. as important pollinators, bumblebees must learn to forage on flowers to feed both themselves and provision their colonies.increased use of pesticides has caused concern over sublethal effects on bees, such as impacts on reproduction or learning ability. however, little is known about how sublethal exposure to field-realistic levels of pesticide might affect the abil ... | 2016 | 27512241 |
| effect of exchanging onobrychis viciifolia and lotus corniculatus for medicago sativa on ruminal fermentation and nitrogen turnover in dairy cows. | the objective of the study was to determine the effect of feeding sainfoin (sf; onobrychis viciifolia) and birdsfoot trefoil (bt; lotus corniculatus), 2 temperate climate forage legumes that contain condensed tannins (ct), on ruminal fermentation and n turnover in dairy cows. six ruminally cannulated multiparous dairy cows (milk yield=40kg/d; 36 d in milk) were used in a replicated 3×3 latin square design. all animals were fed basal diets containing 20% pelleted sf (223g of ct/kg of dry matter), ... | 2016 | 26995129 |
| plants increase arsenic in solution but decrease the non-specifically bound fraction in the rhizosphere of an alkaline, naturally rich soil. | we aimed at determining the major physical-chemical processes that drive arsenic (as) dynamic in the rhizosphere of four species (holcus lanatus, dittrichia viscosa, lotus corniculatus, plantago lanceolata) tested for phytostabilization. experiments were performed with an alkaline soil naturally rich in as. composition of the soil solution of planted and unplanted pots was monitored every 15 days for 90 days, with a focus on the evolution of as concentrations in solution and in the non-specifica ... | 2016 | 26707185 |
| compost and calcium surface treatment effects on subsoil chemistry in acidic minespoil columns. | surface incorporation of a liming agent in combination with compost or biosolids is a proven way to revegetate acidic minespoils, but little is known about the effect of the surface amendments on subsoil chemistry. we conducted a greenhouse column experiment to investigate how different surface amendments affected plant growth and subsoil chemistry in highly acidic minespoil material. columns were filled with shale minespoil material (ph approximately 2.5), amended with caco3, caso4 x 2h2o (gyps ... | 2016 | 12809279 |
| therapeutic potential of temperate forage legumes: a review. | the discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. temperate forage legumes are fabaceae species that include worldwide-important crops. these plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. we have examined here medicago sativa (alfalfa), trif ... | 2016 | 26507574 |
| mesorhizobium olivaresii sp. nov. isolated from lotus corniculatus nodules. | in this study four mesorhizobium strains isolated from lotus corniculatus nodules in granada (spain) were characterized. their 16s rrna gene sequences were closely related to those of m. albiziae lmg 23507(t) and m. chacoense pr5(t) showing 99.4 and 99.2% similarity values, respectively. the analysis of concatenated rpob, reca, atpd and glnii genes showed they formed a cluster with internal similarities higher than 97%. the closest species also were m. albiziae lmg 23507(t) and m. chacoense pr5( ... | 2016 | 27712916 |
| carcass parameters and meat quality in meat-goat kids finished on chicory, birdsfoot trefoil, or red clover pastures. | this study was conducted during the 2009 and 2010 grazing seasons to assess carcass parameters and chevon (goat meat) quality when meat-goat kids (n=72) were finished on pastures of red clover (trifolium pratense l.; rcl), birdsfoot trefoil (lotus corniculatus l.; bft), or chicory (cichorium intybus l.; chic). final live weight (p<0.05) and carcass weight (p=0.10) were greater when goats were finished on rcl compared to chic with bft being intermediate. ribeye area, backfat thickness, body wall ... | 2015 | 25817803 |
| interrelations between herbage yield, α-tocopherol, β-carotene, lutein, protein, and fiber in non-leguminous forbs, forage legumes, and a grass-clover mixture as affected by harvest date. | pastures with diverse botanical composition may enhance animal-derived product quality. a recent study demonstrated high vitamin concentrations and yields in some forb species. the objectives of the present study were to investigate interrelations between herbage yields, vitamin concentrations, protein and fiber contents and analyze the effect of harvest date. we hypothesized that interrelations would be similar across investigated forage species. four nonleguminous forbs: salad burnet (sanguiso ... | 2015 | 25573460 |
| soil-to-plant transfer of native selenium for wild vegetation cover at selected locations of the czech republic. | total selenium (se) contents were determined in aboveground biomass of wild plant species growing in two uncultivated meadows at two different locations. the soils in these locations had pseudototal (aqua regia soluble) se in concentration ranges of between 0.2 and 0.3 mg kg(-1) at the first location, and between 0.7 and 1.4 mg kg(-1) at the second location. the plant species represented 29 plant families where the most numerous ones were poaceae, rosaceae, fabaceae , and asteraceae. the seleniu ... | 2015 | 25975239 |
| revision of the taxonomic status of type strains of mesorhizobium loti and reclassification of strain usda 3471t as the type strain of mesorhizobiumerdmanii sp. nov. and atcc 33669t as the type strain of mesorhizobiumjarvisii sp. nov. | the species mesorhizobim loti was isolated from nodules of lotus corniculatus and its type strain deposited in several collections. some of these type strains, such as those deposited in the usda and atcc collections before 1990, are not coincident with the original strain, nzp 2213t, deposited in the nzp culture collection. the analysis of the 16s rrna gene showed that strains usda 3471t and atcc 33669t formed independent branches from that occupied by mesorhizobium loti nzp 2213t and related t ... | 2015 | 25736411 |
| growth and survival of mesorhizobium loti inside acanthamoeba enhanced its ability to develop more nodules on lotus corniculatus. | the importance of protozoa as environmental reservoirs of pathogens is well recognized, while their impact on survival and symbiotic properties of rhizobia has not been explored. the possible survival of free-living rhizobia inside amoebae could influence bacterial abundance in the rhizosphere of legume plants and the nodulation competitiveness of microsymbionts. two well-characterized strains of mesorhizobium: mesorhizobium loti nzp2213 and mesorhizobium huakuii symbiovar loti maff303099 were a ... | 2015 | 25779926 |
| genome sequence of the lotus spp. microsymbiont mesorhizobium loti strain r7a. | mesorhizobium loti strain r7a was isolated in 1993 in lammermoor, otago, new zealand from a lotus corniculatus root nodule and is a reisolate of the inoculant strain icmp3153 (nzp2238) used at the site. r7a is an aerobic, gram-negative, non-spore-forming rod. the symbiotic genes in the strain are carried on a 502-kb integrative and conjugative element known as the symbiosis island or icemlsym(r7a). m. loti is the microsymbiont of the model legume lotus japonicus and strain r7a has been used exte ... | 2014 | 25780499 |
| genome sequence of the lotus corniculatus microsymbiont mesorhizobium loti strain r88b. | mesorhizobium loti strain r88b was isolated in 1993 in the rocklands range in otago, new zealand from a lotus corniculatus root nodule. r88b is an aerobic, gram-negative, non-spore-forming rod. this report reveals the genome of m. loti strain r88b contains a single scaffold of size 7,195,110 bp which encodes 6,950 protein-coding genes and 66 rna-only encoding genes. this genome does not harbor any plasmids but contains the integrative and conjugative element icemlsym(r7a), also known as the r7a ... | 2014 | 25780496 |
| in vitro screening of forty medicinal plant extracts from the united states northern great plains for anthelmintic activity against haemonchus contortus. | an egg hatch assay (eha) and a larval migration assay (lma) involving haemonchus contortus was used to evaluate the anthelmintic activity of methanol extracts from 40 plants that are native or naturalized within the u.s.a. northern great plains. only one of these 40 plants (i.e. lotus corniculatus) had been previously evaluated for activity against any gastrointestinal nematode. the various extracts were initially screened at 50mg/ml diluted either in 0.5% dimethyl sulphoxide (dmso) or 3-(n-morp ... | 2014 | 24548703 |
| the trehalose utilization gene thua ortholog in mesorhizobium loti does not influence competitiveness for nodulation on lotus spp. | competitiveness for nodulation is a desirable trait in rhizobia strains used as inoculant. in sinorhizobium meliloti 1021 mutation in either of the trehalose utilization genes thua or thub influences its competitiveness for root colonization and nodule occupancy depending on the interacting host. we have therefore investigated whether mutation in the thua ortholog in mesorhizobium loti maff303099 also leads to a similar competitive phenotype on its hosts. the results show that m. loti thua mutan ... | 2014 | 24142427 |
| phyllobacterium loti sp. nov. isolated from nodules of lotus corniculatus. | strain s658(t) was isolated from a lotus corniculatus nodule in a soil sample obtained in uruguay. phylogenetic analysis of the 16s rrna gene and atpd gene showed that this strain clustered within the genus phyllobacterium. the closest related species was, in both cases, phyllobacterium trifolii petp02(t) with 99.8 % sequence similarity in the 16s rrna gene and 96.1 % in the atpd gene. the 16s rrna gene contains an insert at the beginning of the sequence that has no similarities with other inser ... | 2014 | 24271211 |
| does the invasive lupinus polyphyllus increase pollinator visitation to a native herb through effects on pollinator population sizes? | invasive plants may compete with native species for abiotic factors as light, space and nutrients, and have also been shown to affect native pollination interactions. studies have mainly focused on how invasive plants affect pollinator behaviour, i.e. attraction of pollinators to or away from native flowers. however, when an invasive plant provides resources utilized by native pollinators this could increase pollinator population sizes and thereby pollination success in natives. effects mediated ... | 2014 | 24061551 |
| determination of nutrient contents and in vitro gas production values of some legume forages grown in the harran plain saline soils. | the aim of this study was to determine the nutritive value of some legume species in salt-affected soils of south-east anatolian region using chemical composition and in vitro gas production kinetics. in this study, lotus corniculatus, trifolium alexandrinum, medicago sativa were sown and tested in four different locations. a 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (ec)<4 ds/m; low salt: 4 ds/m>ec<8 ds/m, medium saline: 8 ds/m>ec<16 ds/m ... | 2014 | 25050020 |
| genome sequence of ensifer arboris strain lmg 14919(t); a microsymbiont of the legume prosopis chilensis growing in kosti, sudan. | ensifer arboris lmg 14919(t) is an aerobic, motile, gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of several species of legume trees. lmg 14919(t) was isolated in 1987 from a nodule recovered from the roots of the tree prosopis chilensis growing in kosti, sudan. lmg 14919(t) is highly effective at fixing nitrogen with p. chilensis (chilean mesquite) and acacia senegal (gum arabic tree or gum acacia). lmg 14919(t) does not nodulate the tree ... | 2014 | 25197433 |
| fatty acids, α-tocopherol, β-carotene, and lutein contents in forage legumes, forbs, and a grass-clover mixture. | fresh forages are an important natural source of vitamins and fatty acids in ruminant diets, and their concentrations in forage species are important for the quality of animal-derived foods such as dairy and meat products. the aims of this study were to obtain novel information on vitamins and fatty acids (fa) in a variety of forage legumes and non-legume forb species compared to a grass-clover mixture and to explore implications for animal-derived products. seven dicotyledons [four forbs (salad ... | 2013 | 24262011 |
| antioxidant and photosystem ii responses contribute to explain the drought-heat contrasting tolerance of two forage legumes. | identification of metabolic targets of environmental stress factors is critical to improve the stress tolerance of plants. studying the biochemical and physiological responses of plants with different capacities to deal with stress is a valid approach to reach this objective. lotus corniculatus (lotus) and trifolium pratense (clover) are legumes with contrasting summer stress tolerances. in stress conditions, which are defined as drought, heat or a combination of both, we found that differential ... | 2013 | 23792824 |
| in situ protein degradation of alfalfa and birdsfoot trefoil hays and silages as influenced by condensed tannin concentration. | dairy cattle often make poor use of protein when offered diets comprising high proportions of alfalfa (medicago sativa l.) hay or silage because nonprotein n formed during forage conservation and ruminal fermentation exceeds requirements for rumen microbial protein synthesis; however, condensed tannins (ct) may reduce proteolysis in the silo and in the rumen, thereby potentially improving the efficiency of crude protein (cp) use in ruminant diets. two harvests, yielding 12 hays and 12 silages ma ... | 2013 | 23477815 |
| conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis. | rhizobial surface polysaccharides are required for nodule formation on the roots of at least some legumes but the mechanisms by which they act are yet to be determined. as a first step to investigate the function of exopolysaccharide (eps) in the formation of determinate nodules, we isolated mesorhizobium loti mutants affected in various steps of eps biosynthesis and characterized their symbiotic phenotypes on two lotus spp. the wild-type m. loti r7a produced both high molecular weight eps and l ... | 2013 | 23134480 |
| suitability of live yeast addition to alleviate the adverse effects due to the restriction of the time of access to feed in sheep fed only pasture. | the effect of yeast addition on intake and digestive utilization of pasture was studied in ovines under restricted time of access to forage. eighteen wethers housed in metabolic cages and fed fresh forage (predominantly lotus corniculatus) were randomly assigned to three treatments: forage available all day (ad); forage available only 6 h/day (r) and forage available only 6 h/day plus live saccharomyces cerevisiae yeast (ry). feed intake and digestibility, feeding behaviour, kinetics of passage, ... | 2013 | 23020124 |
| improvement of drought and salt tolerance in arabidopsis and lotus corniculatus by overexpression of a novel dreb transcription factor from populus euphratica. | a novel dreb (dehydration-responsive element binding) gene, designated pedreb2a, was isolated from the desert-grown tree, populus euphratica oliv. pedreb2a is classified into the a-5 group of dreb subfamily based on multiple sequence alignment and phylogenetic characterization. using semi-quantitative rt-pcr, we found that the pedreb2a was greatly induced by drought, nacl, low temperature, 1-naphthaleneacetic acid (naa), 6-benzyl aminopurine (6-ba) and gibberellic acid (ga3) treatments in p. eup ... | 2012 | 22771912 |
| increased plant carbon translocation linked to overyielding in grassland species mixtures. | plant species richness and productivity often show a positive relationship, but the underlying mechanisms are not fully understood, especially at the plant species level. we examined how growing plants in species mixture influences intraspecific rates of short-term carbon (c-) translocation, and determined whether such short-term responses are reflected in biomass yields. we grew monocultures and mixtures of six common c3 grassland plant species in outdoor mesocosms, applied a (13)c-co(2) pulse ... | 2012 | 23049893 |
| two years research on efficiency of two intercrops, birdsfoot trefoil and summer savory, to reduce damage caused by onion thrips(thrips tabaci lindeman, thysanoptera, thripidae) on leek. | in 2009 and 2011, a field experiment was carried out at the laboratory field at the biotechnical faculty in ljubljana, slovenia, with the aim to investigate suitability of two intercrops, birdsfoot trefoil (lotus corniculatus l) and summer savory (satureja hortensis l.), for reducing damage caused by onion thrips (thrips tabaci lindeman) on leek (allium porrum l.). four leek cultivars--'columbus', 'forrest', 'lancelot' and 'lincoln'--were used in the research (bejo zaden b.v., netherlands). in b ... | 2012 | 23885428 |
| polymorphic infection and organogenesis patterns induced by a rhizobium leguminosarum isolate from lotus root nodules are determined by the host genotype. | to sample the natural variation in genes controlling compatibility in the legume-rhizobium symbiosis, we isolated rhizobia from nodules of endemic lotus species from 21 sites across europe. the majority of isolates were identified as mesorhizobium- or bradyrhizobium-related and formed nitrogen-fixing root nodules on lotus corniculatus and l. pendunculatus, respectively, thus confirming previously defined cross-inoculation groups. rhizobium leguminosarum (rl) strain norway, isolated from l. corni ... | 2012 | 22950721 |
| herbaceous vegetation productivity, persistence, and metals uptake on a biosolids-amended mine soil. | the selection of plant species is critical for the successful establishment and long-term maintenance of vegetation on reclaimed surface mined soils. a study was conducted to assess the capability of 16 forage grass and legume species in monocultures and mixes to establish and thrive on a reclaimed appalachian surface mine amended with biosolids. the 0.15-ha coarse-textured, rocky, non-acid forming mined site was prepared for planting by grading to a 2% slope and amending sandstone overburden ma ... | 2012 | 16151233 |
| an integrated approach to demonstrating the anr pathway of proanthocyanidin biosynthesis in plants. | proanthocyanidins (pas) are oligomers or polymers of plant flavan-3-ols and are important to plant adaptation in extreme environmental conditions. the characterization of anthocyanidin reductase (anr) and leucoanthocyanidin reductase (lar) has demonstrated the different biogenesis of four stereo-configurations of flavan-3-ols. it is important to understand whether anr and the anr pathway widely occur in the plant kingdom. here, we report an integrated approach to demonstrate the anr pathway in p ... | 2012 | 22678031 |
| promex - a mass spectral reference database for plant proteomics. | the promex database is one of the main collection of annotated tryptic peptides in plant proteomics. the main objective of the promex database is to provide experimental ms/ms-based information for cell type-specific or sub-cellular proteomes in arabidopsis thaliana, medicago truncatula, chlamydomonas reinhardtii, lotus japonicus, lotus corniculatus, phaseolus vulgaris, lycopersicon esculentum, solanum tuberosum, nicotiana tabacum, glycine max, zea mays, bradyrhizobium japonicum, and sinorhizobi ... | 2012 | 22685450 |
| lotus corniculatus regulates the inflammation induced by bradykinin in a murine model of pleurisy. | this study evaluated the anti-inflammatory efficacy of the crude extract (ce), the fractions derived from hexane (hex), ethyl acetate (acoet), n-butanol (buoh), and aqueous (aq) and isolated compounds (oleanolic acid or kaempferitrin) obtained from the aerial parts of lotus corniculatus var. sa~o gabriel in mice with bradykinin-induced pleurisy. swiss mice were used for the in vivo experiments. inflammatory parameters [leukocytes; exudate concentrations; myeloperoxidase and adenosine-deaminase a ... | 2011 | 21355560 |
| genetic diversity of root nodule bacteria nodulating lotus corniculatus and anthyllis vulneraria in sweden. | very little is known about the genetic diversity and phylogeny of rhizobia nodulating lotus species in northern temperate regions. we have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from lotus corniculatus and anthyllis vulneraria from different geographic sites and habitats in sweden by restriction fragment length polymorphism (rflp) of the internal transcribed spacer between their 16s rrna and 23s rrna (igs) region. a high diversity consisting of ... | 2011 | 21497473 |
| isolation and characterization of two strains of fusarium oxysporum causing potato dry rot in solanum tuberosum in colombia. | background: fusarium oxysporum has worldwide distribution and causes severe vascular wilt or root rot in many plants. strains are classified into formae speciales based on their high degree of host specificity, of which multilocus sequence typing provides a fairly good estimate. aims: the main aim of this study was to identify the causal agent of an infected potato tuber in colombia. methodology and results: two f. oxysporum isolates were recovered from a potato tuber showing symptoms of dry rot ... | 2011 | 21635960 |
| condensed tannins act against cattle nematodes. | the use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (gin) in ruminants. direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat gin. these anthelmintic properties are mainly associated with condensed tannins. in the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine gin. effects of onobrychis viciifolia, lotus pedunculatus and lotus cornic ... | 2011 | 21726942 |
| phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes. | lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ... | 2011 | 22092879 |
| characterization of strains unlike mesorhizobium loti that nodulate lotus spp. in saline soils of granada, spain. | lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. the aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with lotus spp. a total of 180 isolates able to nodulate lotus corniculatus and lotus tenuis from two locations in granada, spain, were characterized. molecular identificatio ... | 2010 | 20435777 |
| lotus tenuis tolerates combined salinity and waterlogging: maintaining o2 transport to roots and expression of an nhx1-like gene contribute to regulation of na+ transport. | salinity and waterlogging interact to reduce growth for most crop and pasture species. the combination of these stresses often cause a large increase in the rate of na(+) and cl(-) transport to shoots; however, the mechanisms responsible for this are largely unknown. to identify mechanisms contributing to the adverse interaction between salinity and waterlogging, we compared two lotus species with contrasting tolerances when grown under saline (200 mm nacl) and o(2)-deficient (stagnant) treatmen ... | 2010 | 20444189 |
| the effects of condensed tannins extracted from different plant species on egg hatching and larval development of teladorsagia circumcincta (nematoda: trichostrongylidae). | the effects of condensed tannins (cts) extracted from five species of plants on egg hatching and larval development of teladorsagia circumcincta (stadelmann, 1894) (syn. ostertagia circumcincta) were evaluated using in vitro bioassays. the extracts of cts were obtained from lotus pedunculatus (lp), lotus corniculatus (lc), dorycnium pentaphyllum (dp), dorycnium rectum (dr) and rumex obtusifolius (ro). the results of egg hatching assay showed that about 53%, 68%, 51%, 60% and 46% of the eggs hatc ... | 2010 | 20450000 |
| hydrocarbon degradation and plant colonization by selected bacterial strains isolated from italian ryegrass and birdsfoot trefoil. | to assess the degradation potential and plant colonization capacity of four alkane-degrading strains (itsi10, itri15, itrh76 and btrh79) in combination with birdsfoot trefoil and italian ryegrass and to evaluate the diversity of indigenous alkane-degrading soil bacteria in the rhizo- and endosphere. | 2010 | 20522148 |
| metabolomics unravel contrasting effects of biodiversity on the performance of individual plant species. | in spite of evidence for positive diversity-productivity relationships increasing plant diversity has highly variable effects on the performance of individual plant species, but the mechanisms behind these differential responses are far from being understood. to gain deeper insights into the physiological responses of individual plant species to increasing plant diversity we performed systematic untargeted metabolite profiling on a number of herbs derived from a grassland biodiversity experiment ... | 2010 | 20830202 |
| fox-superroots of lotus corniculatus, overexpressing arabidopsis full-length cdna, show stable variations in morphological traits. | using the full-length cdna overexpressor (fox) gene-hunting system, we have generated 130 arabidopsis fox-superroot lines in bird's-foot trefoil (lotus corniculatus) for the systematic functional analysis of genes expressed in roots and for the selection of induced mutants with interesting root growth characteristics. we used the arabidopsis-fox agrobacterium library (constructed by ligating pbig2113sf) for the agrobacterium-mediated transformation of superroots (sr) and the subsequent selection ... | 2010 | 21106274 |
| two bee-pollinated plant species show higher seed production when grown in gardens compared to arable farmland. | insect pollinator abundance, in particular that of bees, has been shown to be high where there is a super-abundance of floral resources; for example in association with mass-flowering crops and also in gardens where flowering plants are often densely planted. since land management affects pollinator numbers, it is also likely to affect the resultant pollination of plants growing in these habitats. we hypothesised that the seed or fruit set of two plant species, typically pollinated by bumblebees ... | 2010 | 20668704 |
| performance and dietary preferences of white-tailed deer grazing chicory, birdsfoot trefoil or alfalfa in north central alberta. | little information exists on the performance of deer on alternative forage species in northern temperate environments during summer and fall, the period of inherent maximum growth in deer. in performance and choice experiments, we compared live weight gain (g/kg(0.75)/day), absolute [kg/ha dry matter (dm)] and relative (% dm) herbage utilization, relative preference index (rpi) as well as plant community visitation of white-tailed deer grazing alfalfa (medicago sativa), birdsfoot trefoil (lotus ... | 2009 | 19138349 |
| ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range. | eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ... | 2009 | 19477097 |
| agrobacterium rhizogenes-mediated transformation of superroot-derived lotus corniculatus plants: a valuable tool for functional genomics. | transgenic approaches provide a powerful tool for gene function investigations in plants. however, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. superroot of lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. recently, a technique to obtain transgenic l. corniculatus plants from superroot-derived leaves through a. tu ... | 2009 | 19555486 |
| 454 pyrosequencing based transcriptome analysis of zygaena filipendulae with focus on genes involved in biosynthesis of cyanogenic glucosides. | an essential driving component in the co-evolution of plants and insects is the ability to produce and handle bioactive compounds. plants produce bioactive natural products for defense, but some insects detoxify and/or sequester the compounds, opening up for new niches with fewer competitors. to study the molecular mechanism behind the co-adaption in plant-insect interactions, we have investigated the interactions between lotus corniculatus and zygaena filipendulae. they both contain cyanogenic ... | 2009 | 19954531 |
| short-term nutritional treatments grazing legumes or feeding concentrates increase prolificacy in corriedale ewes. | we tested whether short periods of increased nutrition will improve ovulation rate and prolificacy, irrespective of the method used to synchronise the cycles of the ewes. in experiment 1, we used 138 corriedale ewes to evaluate two factors: synchronization treatment (sponges versus a single injection of prostaglandin) and type of pasture (native versus improved with lotus corniculatus). ewes were mated at the end of the grazing period and prolificacy was evaluated at lambing. grazing lotus corni ... | 2009 | 18639397 |
| functional analysis of three ahl autoinducer synthase genes in mesorhizobium loti reveals the important role of quorum sensing in symbiotic nodulation. | one of the most important signal transduction pathways in bacteria, quorum sensing, is involved in many regulatory circuits in rhizobia, especially in the control of communication between rhizobia and their plant hosts. in this study, we identified 3 autoinducer synthase genes - mrli1, mrli2, and mrli3 - in mesorhizobium loti nzp 2213. we found that mrli1 and mrli2 could synthesize distinct n-acyl homoserine lactone (ahl) autoinducers in rich medium cultures, and the expression of mrli1 was show ... | 2009 | 19295655 |
| technical note: effects of forage protein-binding polyphenols on chemistry of dairy excreta. | forage chemistry can affect intake, digestion, milk production, and manure excretion. although information is available on the effects of forage protein-binding polyphenols on small ruminant production and manure excretion, little information is available for dairy cattle. the objective of this study was to compare fecal and urinary n excretion of diets formulated with alfalfa (medicago sativa l.) silage versus condensed tannin-containing birdsfoot trefoil (lotus corniculatus) or o-quinone-conta ... | 2009 | 19307659 |
| the incomplete substitution of lipopolysaccharide with o-chain prevents the establishment of effective symbiosis between mesorhizobium loti nzp2213.1 and lotus corniculatus. | mesorhizobium loti nzp2213.1 mutant obtained after random tn5 mutagenesis of m. loti nzp2213 was inefficient in nitrogen fixation on lotus corniculatus. the transposon insertion was located within an orf with a sequence similarity to a putative glycosyl transferase from caulobacter crescentus. the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the mutant produced lps of the same o-chain length but only half of the entire smooth lps, compared to that of the pa ... | 2009 | 17321732 |
| two glutamine synthetase genes from phaseolus vulgaris l. display contrasting developmental and spatial patterns of expression in transgenic lotus corniculatus plants. | the gln-gamma gene, which specifies the gamma subunit of glutamine synthetase in phaseolus vulgaris l., has been isolated and the regulatory properties of its promoter region analyzed in transgenic lotus corniculatus plants. a 2-kilobase fragment from the 5'-flanking region of gln-gamma conferred a strongly nodule-enhanced pattern of expression on the beta-glucuronidase reporter gene. parallel studies on the promoter of another glutamine synthetase gene (gln-beta) showed that a 1.7-kilobase frag ... | 2008 | 2577727 |
| mesorhizobium gobiense sp. nov. and mesorhizobium tarimense sp. nov., isolated from wild legumes growing in desert soils of xinjiang, china. | twenty-four mesorhizobium strains were isolated from desert soils in the xinjiang region of china and were characterized by a polyphasic approach. these strains grouped into three clusters in igs-rflp, sds-page analysis of whole-cell proteins and box-pcr analysis, corresponding to genomic species v, vi and vii as found in a previous study. the results were supported by sequencing analyses of rrs, igs, atpd and reca genes. genospecies vii was most related to mesorhizobium septentrionale, while ge ... | 2008 | 18984702 |
| a high-throughput method for the quantification of proanthocyanidins in forage crops and its application in assessing variation in condensed tannin content in breeding programmes for lotus corniculatus and lotus uliginosus. | lotus corniculatus and lotus uliginosus are agronomically important forage crops used in ruminant livestock production. the condensed tannin (ct) content, dry matter (dm) production, and persistence of these species are key characteristics of interest for future exploitation of these crops. here we present field data on 19 varieties of l. corniculatus, 2 varieties of l. uliginosus and, additionally, a glasshouse experiment using 6 varieties of l. corniculatus and 2 varieties of l. uliginosus. cu ... | 2008 | 18193833 |
| alteration of o-specific polysaccharide structure of symbiotically defective mesorhizobium loti mutant 2213.1 derived from strain nzp2213. | mesorhizobium loti mutant 2213.1 derived from the wild-type strain nzp2213 by tn5 mutagenesis showed impaired effectiveness of symbiosis with the host plant lotus corniculatus (turska-szewczuk et al., 2007 microbiol res, in press). the inability of lipopolysaccharide (lps) isolated from the mutant 2213.1 strain or de-o-acetylated lps of the parental cells to inactivate phage a1 particles implicated alterations in the lps structure. the o-specific polysaccharide of the mutant was studied by chemi ... | 2008 | 18217107 |
| forage systems for cow-calf production in the appalachian region. | small cow-calf operations are common in the appalachian region. tall fescue [lolium arundinaceum (schreb.) s. j. darbyshire] is the dominant forage in these systems for direct grazing as well as for stockpiling. the present study was conducted from 2001 to 2005. a total of 108 angus and angus crossbred cows were allotted randomly to 6 forage systems and then to 3 replicates within each system. in brief, system 1 had a stocking rate of 0.91 ha/cow in a middleburg 3-paddock (a, b, and c) system. s ... | 2008 | 18407993 |
| transgenic superroots of lotus corniculatus can be regenerated from superroot-derived leaves following agrobacterium-mediated transformation. | super-growing roots (superroots; sr), which have been established in the legume species lotus corniculatus, are a fast-growing root culture that allows continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely growth regulator-free culture conditions. these features are unique for non-hairy root cultures, and they are now stably expressed since the culture was isolated more than 10 years ago (1997). attempts to achieve direct and stable transformation o ... | 2008 | 18471930 |
| ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in lotus corniculatus. | proanthocyanidins (pas) are plant secondary metabolites and are composed primarily of catechin and epicatechin units in higher plant species. due to the ability of pas to bind reversibly with plant proteins to improve digestion and reduce bloat, engineering this pathway in leaves is a major goal for forage breeders. here, we report the cloning and expression analysis of anthocyanidin reductase (anr) and leucoanthocyanidin 4-reductase (lar), two genes encoding enzymes committed to epicatechin and ... | 2007 | 17098849 |
| biomass allocation is an important determinant of the tannin concentration in growing plants. | condensed tannins (cts) in the diet affect consumers in a concentration-dependent manner. because of their importance in plant defence against herbivores and pathogens as well as their potential application against gastrointestinal parasites of ruminants in agronomy, an understanding of the seasonal dynamics of ct concentrations during plant growth is essential. | 2007 | 17210606 |
| palatability in sheep and in vitro nutritional value of dried and ensiled sainfoin (onobrychis viciifolia) birdsfoot trefoil (lotus corniculatus), and chicory (cichorium intybus). | three temperate forages, sainfoin, birdsfoot trefoil, and chicory, characterized by elevated contents of plant secondary compounds, were compared to a ryegrass-clover mixture (control) in dried (experiment 1) and ensiled form (experiment 2) in their palatability and nutritional value. palatability was measured in adult wethers (n = 6) allowed to choose between the familiar control forage and one of the three test plants. palatability index was calculated from differences in intake of control and ... | 2007 | 18069619 |
| identification and subcellular localization of the soybean copper p1b-atpase gmhma8 transporter. | we have identified a copper p(1b)-atpase transporter in soybean (glycine max), named as gmhma8, homologue to cyanobacterial pacs and arabidopsis thaliana athma8 (paa2) transporters. a novel specific polyclonal anti-gmhma8 antibody raised against a synthetic peptide reacted with a protein of an apparent mass of around 180-200 kda in chloroplast and thylakoid membrane preparations isolated from soybean cell suspensions. immunoblot analysis with this antibody also showed a band with similar apparen ... | 2007 | 17169574 |
| individual administration of three tanniferous forage plants to lambs artificially infected with haemonchus contortus and cooperia curticei. | we investigated direct anthelmintic effects associated with the feeding of fresh tanniferous forages against established populations of haemonchus contortus and cooperia curticei in lambs. twenty-four parasite naive lambs were inoculated with a single dose of infective larvae of these two parasites 27 days prior to the start of the feeding experiment. lambs were individually fed with either chicory (cichorium intybus), birdsfoot trefoil (lotus corniculatus), sainfoin (onobrychis viciifolia) or a ... | 2007 | 17336459 |
| no-choice preference of cerotoma trifurcata (coleoptera: chrysomelidae) to potential host plants of bean pod mottle virus (comoviridae) in iowa. | to better understand the naturally occurring host range of bean pod mottle virus (family comoviridae, genus comovirus, bpmv) and its principal vector cerotoma trifurcata (förster) (coleoptera: chrysomelidae), 18 field-collected perennial plant species were tested for the presence of bpmv. by using no-choice assays, we determined the preference of these plants by bean leaf beetle, by measuring their level of herbivory relative to soybean, glycine max (l.). new food hosts for adult bean leaf beetl ... | 2007 | 17598542 |
| toxicity of phenolic compounds extracted from bark residues of different ages. | in quebec, canada, industrial bark wastelands cover several hundred hectares of land. bark residue that has piled up for decades tends to remain free of vegetation for years. to assess the revegetation potential of such sites, we sought to determine those factors responsible for poor plant growth. phenolic compounds from fresh to 20-year-old bark residues were extracted with four solvents and quantified by high-performance liquid chromatography (hplc). we simulated solutions (mixtures of standar ... | 2006 | 17089184 |
| the effects of birdsfoot trefoil (lotus corniculatus) and chicory (cichorium intybus) when compared with perennial ryegrass (lolium perenne) on ovine gastrointestinal parasite development, survival and migration. | two experiments were conducted to investigate the effects of birdsfoot trefoil and chicory on parasitic nematode development, survival and migration when compared with perennial ryegrass. in experiment one, sheep faeces, containing 10,385 cooperia curticei eggs were added to 25 cm diameter pots containing birdsfoot trefoil, chicory or ryegrass, and the pots maintained under optimal conditions for nematode parasite development. replicate pots of each forage type were destructively sampled on day ... | 2006 | 16495015 |
| a comparison of two strategies to modify the hydroxylation of condensed tannin polymers in lotus corniculatus l. | a full-length sense antirrhinum majus dihydroflavonol reductase (dfr) sequence was introduced into birdsfoot trefoil (lotus corniculatus l.) in experiments aimed at modifying condensed tannin content and polymer hydroxylation in a predictable manner. analysis of transgenic plants indicated lines that showed enhanced tannin content in leaf and stem tissues. in contrast to previous data from root cultures, levels of propelargonidin units were not markedly elevated in lines with enhanced tannin con ... | 2005 | 15896367 |
| de novo alanine synthesis by bacteroids of mesorhizobium loti is not required for nitrogen transfer in the determinate nodules of lotus corniculatus. | deletion of both alanine dehydrogenase genes (alda) in mesorhizobium loti resulted in the loss of alda enzyme activity from cultured bacteria and bacteroids but had no effect on the symbiotic performance of lotus corniculatus plants. thus, neither indeterminate pea nodules nor determinate l. corniculatus nodules export alanine as the sole nitrogen secretion product. | 2005 | 16030244 |
| characterization of condensed tannins purified from legume forages: chromophore production, protein precipitation, and inhibitory effects on cellulose digestion. | to identify simple screening tools for selecting condensed tannin (ct)-containing forages as candidate sources for further study, ct were isolated from nine legumes, and their molecular weights (mw), chromophore production, capacity to precipitate bovine serum albumin (bsa) and fraction 1 protein (rubisco) isolated from alfalfa, and inhibition of filter paper digestion were compared. sources were as follows: leaves of sericea lespedeza (lespedeza cuneata dum.-cours.), crown vetch (coronilla vari ... | 2005 | 16132212 |
| extra thin alginate film: an efficient technique for protoplast culture. | this paper reports an efficient protoplast culture technique, the "extra thin alginate film" technique. the development of this improved method of protoplast culture was an outcome of an assessment of the efficiency and shortcomings of various protoplast culture techniques. the efficiency of this technique was evaluated with two model plant systems, viz., nicotiana tabacum and lotus corniculatus, and a comparison was made with the "thin alginate layer" technique, another efficient protoplast cul ... | 2005 | 16244810 |
| n-hexanoyl-l-homoserine lactone, a mediator of bacterial quorum-sensing regulation, exhibits plant-dependent stability and may be inactivated by germinating lotus corniculatus seedlings. | the half-life of n-hexanoyl-l-homoserine lactone (c6-hsl) was determined under various ph and temperature conditions, and in several plant environments. c6-hsl was sensitive to alkaline ph, a process that was also temperature-dependent. in addition, c6-hsl disappeared from plant environments, i.e. axenic monocot and dicot plants cultivated under gnotobiotic, hydroponic conditions, albeit with variable kinetics. the disappearance was rapid at the root system of legume plants such as clover or lot ... | 2004 | 16329888 |
| symbiotic phenotypes and translocated effector proteins of the mesorhizobium loti strain r7a virb/d4 type iv secretion system. | the symbiosis island of mesorhizobium loti strain r7a contains genes with strong similarity to the structural vir genes (virb1-11; vird4) of agrobacterium tumefaciens that encode the type iv secretion system (t4ss) required for t-dna transfer to plants. in contrast, m. loti strain maff303099 lacks these genes but contains genes not present in strain r7a that encode a type iii secretion system (t3ss). here we show by hybridization analysis that most m. loti strains contain the virb/d4 t4ss and no ... | 2004 | 15469524 |
| lotus hairy roots expressing inducible arginine decarboxylase activity. | biotechnological uses of plant cell-tissue culture usually rely on constitutive transgene expression. however, such expression of transgenes may not always be desirable. in those cases, the use of an inducible promoter could be an alternative approach. to test this hypothesis, we developed two binary vectors harboring a stress-inducible promoter from arabidopsis thaliana, driving the beta-glucuronidase reporter gene and the oat arginine decarboxylase. transgenic hairy roots of lotus corniculatus ... | 2004 | 15195973 |
| the effect of birdsfoot trefoil (lotus corniculatus) and chicory (cichorium intybus) on parasite intensities and performance of lambs naturally infected with helminth parasites. | conventionally, farmers rely upon the routine use of anthelmintics to control helminth parasites and their use has proved highly cost-effective. however, several factors, including the emergence of helminths resistant to pharmaceutical anthelmintics, are forcing farmers to seek alternative approaches to parasite control. studies in new zealand have shown that some alternative forages may reduce parasitic infestation in sheep. in the current study, it was found that under uk environmental conditi ... | 2003 | 12581592 |
| measuring gene flow from two birdsfoot trefoil (lotus corniculatus) field trials using transgenes as tracer markers. | genetic engineering is becoming a useful tool in the improvement of plants but concern has been expressed about the potential environmental risks of releasing genetically modified (gm) organisms into the environment. attention has focused on pollen dispersal as a major issue in the risk assessment of transgenic crop plants. in this study, pollen-mediated dispersal of transgenes via cross-fertilization was examined. plants of lotus corniculatus l. transformed with either the escherichia coli aspa ... | 2003 | 12755896 |
| effect of flavan-3-ols on in vitro egg hatching, larval development and viability of infective larvae of trichostrongylus colubriformis. | the effects of flavan-3-ols (the monomer units of condensed tannins (ct)) and their galloyl derivatives on the viability of eggs, the development of first stage (l1) larvae, and the viability of the infective larvae of trichostrongylus colubriformis were investigated under in vitro conditions. each of the flavan-3-ol gallates showed some inhibition of egg hatching at 100 microg/ml, and 100% inhibition at 1000 microg/ml, with epigallocatechin gallate being the most effective in the egg hatch (eh) ... | 2003 | 14636684 |
| the effect of dietary forage on the development and survival of helminth parasites in ovine faeces. | studies in new zealand and the uk have shown that lambs grazing birdsfoot trefoil (lotus corniculatus) or chicory (cichorium intybus) have reduced parasite intensities compared to lambs grazing ryegrass swards. however, data in the literature on the influence of forages on helminth parasites is equivocal and the underlying mechanisms by which different forage diets may affect these parasites have not been fully determined. the aim of the experiments reported here was to investigate the hypothesi ... | 2003 | 14651879 |
| effect of condensed tannins on egg hatching and larval development of trichostrongylus colubriformis in vitro. | the effects of condensed tannins extracted from seven forages on the viability of the eggs and first stage (l1) larvae of the sheep nematode trichostrongylus colubriformis were evaluated in in vitro assays. the extracts of condensed tannins were obtained from lotus pedunculatus (lp), lotus corniculatus (lc), sulla (hedysarum coronarium), sainfoin (onobrychus viciifolia), dorycnium pentaphylum (dp), dorycnium rectum (dr) and dock (rumex obtusifolius). extracts containing 200 to 500 microg/ml redu ... | 2002 | 11837588 |
| the effect of diet fed to lambs on subsequent development of trichostrongylus colubriformis larvae in vitro and on pasture. | contrasting herbage diets were fed to lambs to evaluate their effect on subsequent development of trichostrongylus colubriformis larvae in faeces and on pasture. the diets had either no condensed tannin (ct), lucerne (medicago sativa cv. otaio), white clover (trifolium repens cv. tahora), or had moderate to high concentrations of ct, sulla (hedysarum coronarium cv. grassland aokau), lotus corniculatus (cv. grasslands goldie), l. pedunculatus (cv. grassland maku), dorycnium pentophyllum, and dory ... | 2002 | 11983303 |
| performance of steers grazing rhizomatous and nonrhizomatous birdsfoot trefoil in pure stands and in tall fescue mixtures. | this study investigated the performance of steers grazing rhizomatous birdsfoot trefoil (lotus corniculatus l.) (rbft) compared to nonrhizomatous birdsfoot trefoil (bft) in pure stands or when interseeded with endophyte-free tall fescue (festuca arundinacea schreb.; tf). five forage treatments of rbft, bft, tf, rbft+tf, and bft+tf (four replicate paddocks per treatment) were continuously stocked in spring and fall of 1998 and spring of 1999. grazing for individual treatments was terminated when ... | 2002 | 12162667 |
| lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep. | condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. however, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. the aim of this study was to investigate the specific effects of condensed tannins from lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (lolium perenne ... | 2002 | 12489781 |
| the bio operon on the acquired symbiosis island of mesorhizobium sp. strain r7a includes a novel gene involved in pimeloyl-coa synthesis. | the symbiosis island of mesorhizobium sp. strain r7a is a 500 kb chromosomal genetic element that upon transfer converts nonsymbiotic mesorhizobia to symbionts able to nodulate and fix nitrogen with lotus corniculatus. four genomic species of nonsymbiotic mesorhizobia have been isolated. all were auxotrophic for thiamin and biotin and three were auxotrophic for nicotinate, whereas derivatives of the strains containing the symbiosis island were prototrophic for all three vitamins. in this work, a ... | 2001 | 11320134 |
| the effect of condensed tannins from lotus pedunculatus and lotus corniculatus on the growth of proteolytic rumen bacteria in vitro and their possible mode of action. | five strains of proteolytic rumen bacteria were treated with condensed tannins (ct) purified from lotus pedunculatus and lotus corniculatus to investigate their effect on the growth of these bacteria in vitro. streptococcus bovis ncfb 2476, eubacterium sp. c124b, prevotella bryantii b14, butyrivibrio fibrisolvens h17c, and clostridium proteoclasticum b316t were tested against 200, 400, and 600 microg ct x ml(-1) extracted from l. pedunculatus and l. corniculatus. in the absence of ct, all bacter ... | 2001 | 11547882 |
| the root-nodule symbiosis between sarothamnus scoparius l. and its microsymbionts. | when nitrogen fixing root nodules are formed, sarothamnus scoparius (broom) is inoculated with its microsymbionts. nodules studied under light and electron microscopy exhibited typical indeterminate nodule histology with apical, persistent meristem, age gradient of nodule tissues, and open vascular bundles, and also with some particular features such as: the presence of mitotic activity in the infected meristematic cells, lack of infection threads, distribution of bacteria by process of host cel ... | 2001 | 11816984 |
| symbiosis of astragalus cicer with its microsymbionts: partial nodc gene sequence, host plant specificity, and root nodule structure. | astragalus cicer (cicer milkvetch) nodule bacteria were investigated for host plant specificity and partial nodc gene sequences, whilst their native host was studied for the microscopic structure of root nodules. the strains under investigation formed nodules not only on the original host but also on astragalus glycyphyllos, astragalus sinicus, lotus corniculatus, and phaseolus vulgaris. the nodules induced on the cicer milkvetch were classified as indeterminate and characterized by apical, pers ... | 2000 | 11016697 |
| the effect of birdsfoot trefoil (lotus corniculatus) and white clover (trifolium repens) in mixed pasture swards on incoming and established nematode infections in young lambs. | lotus corniculatus l., a leguminous plant containing condensed tannins, is now being evaluated in sweden as a component of mixed pastures. this is because of its high nutritive value, palatability, modest requirement for water, calcium and phosphorous and particularly because it has the ability to survive harsh weather conditions that typify northern scandinavia. this trial was undertaken to assess the possible parasitological benefits of using l. corniculatus when fed as a minor component in a ... | 2000 | 11234969 |
| a new mesorhizobium loti hambi 1129 phage isolated from polish soil. | phage a1 isolated from the rhizosphere of lotus corniculatus was studied. it had a very narrow host range, as it was active only against mesorhizobium loti hambi 1129. phage a1 was classified as belonging to c bradley's group bacteriophages. the latent period of a1 was 120-130 min and a burst size 13-17 particles per cell. the nature of the phage receptor was examined. lipopolysaccharide from the phage-sensitive strain inactivated phage a1 in contrast to lps from the phage-resistant bacteria. pu ... | 2000 | 10706666 |
| lupine leghemoglobin i: expression in transgenic lotus and tobacco tissues. | the proximal parts of the promoters of the genes for symbiotic-type hemoglobins are generally conserved, but the promoter of the lbi gene of lupine (lulbi) shows some unusual structural features. it lacks typical organ-specific elements characteristic of all the leghemoglobin gene promoters described thus far. we have analysed its functional activity in transgenic lotus corniculatus. a fusion construct between the lbi promoter and the gus reporter gene was expressed mainly in the central zone of ... | 2000 | 10778735 |
| the effect of condensed tannins from seven herbages on trichostrongylus colubriformis larval migration in vitro. | the effects of condensed tannins (ct) extracted from seven forages on the motility of the economically important nematode, trichostrongylus colubriformis (giles, 1892), were evaluated by using a larval migration inhibition (lmi) assay. the assay involved incubation of third stage (l3) exsheathed t. colubriformis larvae with ct extracted from lotus pedunculatus, lotus corniculatus, sulla (hedysarum coronarium), sainfoin (onobrychis viciifolia), dorycnium rectum, dorycnium pentaphyllum and dock (r ... | 2000 | 10833014 |
| characterization of an atrazine-degrading pseudaminobacter sp. isolated from canadian and french agricultural soils. | atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. fourteen bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from soils obtained from two farms in canada and two farms in france. these strains were indistinguishable from each other based on repetitive extragenic palindromic pcr genomic fingerprinting performed with primers eric1r, eric2, and boxa1r. based on 16s rrna sequence analysis of one representativ ... | 2000 | 10877767 |
| effect of forage legumes containing condensed tannins on lungworm (dictyocaulus sp.) and gastrointestinal parasitism in young red deer (cervus elaphus). | to investigate the effect of feeding forage legumes containing condensed tannins (ct) on internal parasitism, red deer calves were fed either lucerne (medicago sativa; 0.1 per cent ct), birdsfoot trefoil (lotus corniculatus; 1.9 per cent ct) or sulla (hedysarum coronarium; 3.5 per cent ct) and trickle-infected with deer-origin gastrointestinal nematode and lungworm (dictyocaulus sp.) larvae for 5 weeks, then slaughtered at 7 weeks. there was a significant negative linear relationship between die ... | 2000 | 10877967 |
| effect of crop rotation and soil cover on alteration of the soil microflora generated by the culture of transgenic plants producing opines. | the culture of transgenic lotus corniculatus plants producing opines, which are bacterial growth substrates, leads to the selection of rhizospheric bacteria able to utilize these substrates. we have investigated the fate of the opine-utilizing community over time under different experimental conditions following elimination of selective pressure exerted by the transgenic plants. these plants were removed from the soil, which was either left unplanted or replanted with wild-type l. corniculatus o ... | 2000 | 10886651 |
| effect of condensed tannins extracted from four forages on the viability of the larvae of deer lungworms and gastrointestinal nematodes. | the inhibitory activity of condensed tannins extracted from four forage legume plants were evaluated by using a larval migration inhibition assay. the first (l1) and third (l3) stages of deer lungworm (dictyocaulus viviparus), and the third stage (l3) of deer gastrointestinal nematodes were incubated with tannins extracted from lotus pedunculatus, lotus corniculatus, sulla (hedysarum coronarium) and sainfoin (onobrychus viciifolia). the tannins extracted from all the forages had inhibitory activ ... | 2000 | 10955893 |
| the implications of condensed tannins on the nutritive value of temperate forages fed to ruminants. | new methodology for measuring forage condensed tannin (ct) content is described and the effects of ct upon forage feeding and nutritive value for ruminant animals are reviewed. ct react with forage proteins in a ph-reversible manner, with reactivity determined by the concentration, structure and molecular mass of the ct. increasing concentrations of ct in lotus corniculatus and lotus pedunculatus reduce the rates of solubilization and degradation of fraction 1 leaf protein in the rumen and incre ... | 1999 | 10999013 |
| a strong constitutive positive element is essential for the ammonium-regulated expression of a soybean gene encoding cytosolic glutamine synthetase. | in order to identify important promoter elements controlling the ammonium-regulated expression of the soybean gene gs15 encoding cytosolic glutamine synthetase, a series of 5' promoter deletions were fused to the gus reporter gene. to allow the detection of positive and negative regulatory elements, a series of 3' deletions were fused to a -90 camv 35s promoter fragment placed upstream of the gus gene. both types of construct were introduced into lotus corniculatus plants and soybean roots via a ... | 1999 | 10092182 |
| a field study of the effects of elevated co(2) on plant biomass and community structure in a calcareous grassland. | the effects of elevated co(2) on plant biomass and community structure have been studied for four seasons in a calcareous grassland in northwest switzerland. this highly diverse, semi-natural plant community is dominated by the perennial grass bromus erectus and is mown twice a year to maintain species composition. plots of 1.3 m(2) were exposed to ambient or elevated co(2) concentrations (n = 8) using a novel co(2) exposure technique, screen-aided co(2) control (sacc) starting in march 1994. in ... | 1999 | 20135159 |
| effects of elevated co2 on flowering phenology and nectar production of nectar plants important for butterflies of calcareous grasslands. | effects of elevated co2 on flowering phenology and nectar production were investigated in trifolium pratense, lotus corniculatus, scabiosa columbaria, centaurea jacea and betonica officinalis, which are all important nectar plants for butterflies. in glasshouse experiments, juvenile plants were exposed to ambient (350 μl l(-1)) and elevated (660 μl l(-1)) co2 concentrations for 60-80 days. elevated co2 significantly enhanced the development of flower buds in c. jacea. b. officinalis flowered ear ... | 1998 | 28307818 |
| genetic manipulation of condensed tannins in higher plants. ii. analysis of birdsfoot trefoil plants harboring antisense dihydroflavonol reductase constructs | we have produced and analyzed transgenic birdsfoot trefoil (lotus corniculatus l.) plants harboring antisense dihydroflavonol reductase (as-dfr) sequences. in initial experiments the effect of introducing three different antisense antirrhinum majus l. dfr constructs into a single recipient genotype (s50) was assessed. there were no obvious effects on plant biomass, but levels of condensed tannins showed a statistical reduction in leaf, stem, and root tissues of some of the antisense lines. trans ... | 1998 | 9501146 |
| lotus corniculatus nodulation specificity is changed by the presence of a soybean lectin gene | plant lectins have been implicated as playing an important role in mediating recognition and specificity in the rhizobium-legume nitrogen-fixing symbiosis. to test this hypothesis, we introduced the soybean lectin gene le1 either behind its own promoter or behind the cauliflower mosaic virus 35s promoter into lotus corniculatus, which is nodulated by r. loti. we found that nodulelike outgrowths developed on transgenic l. corniculatus plant roots in response to bradyrhizobium japonicum, which nod ... | 1998 | 9707526 |
| production, faecal egg counts and worm burdens of ewe lambs which grazed six contrasting forages. | lambs were grazed on monospecific swards of one of six forages, some containing condensed tannins (ct); sulla (hedysarum coronarium), lotus corniculatus and l. pedunculatus and some without ct (lucerne (medicago sativa), plantain (plantago lanceolata) and a ryegrass/white clover pasture (lolium perenne/trifolium repens)) to evaluate effects of forage type on gastrointestinal nematode burdens, faecal egg count as well as liveweight gain (lwg), wool growth and dag formation over a 42-day period. a ... | 1998 | 9877067 |