Publications

In vitro activity of S-(3,4-dichlorobenzyl)isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex.	The aim of this study was to establish the antimicrobial activities of S-(3,4-dichlorobenzyl)isothiourea hydrochloride (A22) and a series of structurally related compounds against multidrug-resistant (MDR) bacteria. The minimum inhibitory concentrations (MICs) of 21 compounds were determined against 18 strains of pathogenic bacteria in addition to Pseudomonas aeruginosa (n=19) and Burkholderia cepacia complex (BCC) (n=20) isolated from the sputa of cystic fibrosis patients. Selected compounds we ...	2012	21993484
dna profiling of stenotrophomonas maltophilia by pcr targeted to its species-specific repetitive palindromic sequences.	the aim of this study was to develop a simple protocol for a pcr-based fingerprinting of stenotrophomonas maltophilia (smreppcr) that utilizes primers complementary to repetitive extragenic palindromic elements (reps) of this micro-organism.	2012	22044359
extracellular serine proteases from stenotrophomonas maltophilia: screening, isolation and heterologous expression in e. coli.	a large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. encapsulation of cells in alginate beads induced protease production. stenotrophomonas maltophilia emerged as best performer under washing conditions. for identification of wash-active proteases, four extracellular serine proteases called stmpr1, stmpr2, stmpr3 and stmpr4 were clon ...	2012	21983234
characterization of phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants.	this study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. results showed that bacterial treatment followed by wetland plants (phragmites cummunis) resulted 94.5% and 96.0% reduction in bod and cod values, respectively. the pcr-rflp analysis showed the presence of stenotrophomonas, enterobacter, pantoea, acinetobacter an ...	2012	22047662
Diversity and assessment of potential risk factors of Gram-negative isolates associated with French cheeses.	The goal of this study was to identify at the species level a large collection of Gram-negative dairy bacteria isolated from milks or semi-hard and soft, smear-ripened cheeses (cheese core or surface samples) from different regions of France. The isolates were then assessed for two risk factors, antibiotic resistance and volatile and non-volatile biogenic amine production in vitro. In total, 173 Gram-negative isolates were identified by rrs and/or rpoB gene sequencing. A large biodiversity was o ...	2012	22029922
Contemporary unconventional clinical use of co-trimoxazole.	Clin Microbiol Infect 2012; 18: 8-17 ABSTRACT: In the late 1960s, the combination of trimethoprim and sulphamethoxazole (co-trimoxazole) was introduced into clinical practice and used to treat many infectious diseases, such as urinary tract infections, respiratory infections, sexually transmitted diseases, Gram-negative sepsis, enteric infections and typhoid fever. Subsequently, co-trimoxazole was reported to be effective against numerous bacterial, fungal and protozoal pathogens, including Noca ...	2012	21851485
exploring the in situ accessibility of small subunit ribosomal rna of members of the domains bacteria and eukarya to oligonucleotide probes.	the principle that the small subunit ribosomal rna (ssu rrna) is generally accessible to oligonucleotide probes designed to have high thermodynamic affinity was tested with stenotrophomonas maltophilia, rhodobacter sphaeroides, bacillus subtilis, and saccharomyces cerevisiae. fluorescein-labeled probes, designed to have δg(overall)°=-14±1 and to avoid the potential of nucleobase-specific quenching, were used to target 20 randomly selected sites in each organism. a site was considered accessible ...	2012	22264799
gut-associated bacteria throughout the life cycle of the bark beetle dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) and their cellulolytic activities.	dendroctonus rhizophagus thomas and bright (curculionidae: scolytinae) is an endemic economically important insect of the sierra madre occidental in mexico. this bark beetle has an atypical behavior within the genus because just one beetle couple colonizes and kills seedlings and young trees of 11 pine species. in this work, the bacteria associated with the dendroctonus rhizophagus gut were analyzed by culture-dependent and culture-independent methods. analysis of 16s rrna sequences amplified di ...	2012	22234511
nagz-dependent and nagz-independent mechanisms for the β-lactamases expression in stenotrophomonas maltophilia.	the β-n-acetylglucosaminidase (nagz), encoded by nagz gene, is a critical enzyme for the basal-level ampc derepression (ampc expression in absence of β-lactam challenge) in ampd and dacb mutants of pseudomonas aeruginosa. three mutants with a phenotype of basal-level l1 and l2 β-lactamase derepression in stenotrophomonas maltophilia have been reported, including kjδdi (ampd(i) mutant), kjδmrca (mrca mutant), and kjδdiδmrca (ampd(i) and mrca double mutant). in this study, nagz of s. maltophilia w ...	2012	22252801
multilocus sequence typing for clonality analysis of antimicrobial-resistant stenotrophomonas maltophilia strains.		2012	22259772
the role of nutrients in the biodegradation of 2,4,6-trinitrotoluene in liquid and soil.	the widely used explosive 2,4,6-trinitrotoluene (tnt) has residues that are potentially explosive, toxic, and mutagenic. tnt and other explosives can be degraded by microorganisms; however, biostimulation is needed for process efficiency. to investigate the effectiveness of using biostimulation to degrade tnt, we added varying concentrations of a nutrient amendment consisting of inorganic salts, plant extracts, and molasses to soil and liquid media. for the inoculum we used a consortium of bacte ...	2012	22245864
stenotrophomonas maltophilia: an emerging global opportunistic pathogen.	summary: stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. the increasing incidence of nosocomial and community-acquired s. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. s. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. infections of s. maltophilia c ...	2012	22232370
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.	abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...	2011	22243438
endowashers: an overlooked risk for possible post-endoscopic infections.	background: prevention of post-endoscopy infections is an important objective to assure patient safety. endowashers, or high throughput irrigation water pumps, are a frequently used device on endoscopes. recommendations published by professional bodies and regulatory health agencies cover not only adequate reprocessing of fiber-endoscopes but also state accepted methods of regular microbial sampling. although major instruments like endoscopes are covered by these recommendations, other devices u ...	2011	22242094
contribution of resistance-nodulation-division efflux pump operon smeu1-v-w-u2-x to multidrug resistance of stenotrophomonas maltophilia.	kj09c, a multidrug-resistant mutant of stenotrophomonas maltophilia kj, was generated by in vitro selection with chloramphenicol. the multidrug-resistant phenotype of kj09c was attributed to overexpression of a resistance nodulation division (rnd)-type efflux system encoded by an operon consisting of five genes: smeu1, smev, smew, smeu2, and smex. proteins encoded by smev, smew, and smex were similar to the membrane fusion protein, rnd transporter, and outer membrane protein, respectively, of kn ...	2011	21930878
multidrug-resistant gram-negative bacterial infections after liver transplantation - spectrum and risk factors.	objectives: gram-negative bacilli infections, especially multidrug-resistant gram-negative bacilli infections, are the leading cause of high mortality after liver transplantation. this study sought to investigate the type of infection, infection rate, pathogenic spectrum, antibiotic-resistance profile, risk factors, and epidemiology of multidrug-resistant gram-negative bacterial infection. methods: a retrospective cohort study was conducted and data of 217 liver transplant patients receiving cad ...	2011	22198738
Characteristics of microbial aerosols released from chicken and swine feces.	Bioaerosols generated during livestock and poultry production are significant occupational hazards. This study investigates the characteristics of bioaerosols released from animal feces. Fresh feces from pigs and chickens were obtained and tested in a controlled-environment facility. Airborne viable (culturable) bacteria and fungi were sampled hourly for 48 hr. The predominant species were identified via polymerase chain reaction analysis. The number of bacterial colonies released from chicken f ...	2011	21874960
Tsunami lung.	We encountered three cases of lung disorders caused by drowning in the recent large tsunami that struck following the Great East Japan Earthquake. All three were females, and two of them were old elderly. All segments of both lungs were involved in all the three patients, necessitating ICU admission and endotracheal intubation and mechanical ventilation. All three died within 3 weeks. In at least two cases, misswallowing of oil was suspected from the features noted at the time of the detection. ...	2011	22057370
Genotyping of environmental and clinical Stenotrophomonas maltophilia isolates and their pathogenic potential.	Stenotrophomonas maltophilia is a highly versatile species with useful biotechnological potential but also with pathogenic properties. In light of possible differences in virulence characteristics, knowledge about genomic subgroups is therefore desirable. Two different genotyping methods, rep-PCR fingerprinting and partial gyrB gene sequencing were used to elucidate S. maltophilia intraspecies diversity. Rep-PCR fingerprinting revealed the presence of 12 large subgroups, while gyrB gene sequenci ...	2011	22110692
Minimal Inhibitory Concentration of Ceftazidime and Co-trimoxazole for Stenotrophomonas Maltophilia using E-test.	Stenotrophomonas maltophilia, previously named as Pseudomonas or Xanthomonas maltophilia, is an important nosocomial pathogen	2011	21887057
[the first experience of application of pcr techniques in real-time mode to diagnose bacteriemia during postoperational period in cardiosurgery patients].	the results comparison is made between pcr polymerase chain reaction techniques in real-time mode and cultural techniques of analysis of blood samples in patients with suspicion of bacteriemia during early postoperational period. from september to december 2009 43 blood samples from 37 adult patients aged 59+9.2 years were analyzed on suspicion of bacteriemia. the blood samples for pcr analysis (septifast technique roche diagnostics, switzerland) were taken parallel to the inoculation. among 43 ...	2011	22164420
The Ax21 protein is a cell-cell signal that regulates virulence in the nosocomial pathogen Stenotrophomonas maltophilia.	Stenotrophomonas maltophilia encodes proteins related to the Rax proteins of Xanthomonas oryzae, which are required for the synthesis and secretion of the Ax21 protein. Here we show that Ax21 acts as a cell-cell signal to regulate a diverse range of functions, including virulence, in this nosocomial pathogen.	2011	21908663
Reducing Sugar-Producing Bacteria from Guts of Tenebrio Molitor Linnaeus (Yellow mealworm) for Lignocellulosic Waste Minimization.	The guts of Tenebrio Molitor Linnaeus (yellow mealworm) were used as inocula to isolate reducing sugar-producing bacteria during bioconversion of lignocellulose to reducing sugars in this study. Three carbon sources, i.e., carboxymethyl cellulose (CMC), filter paper (FP), and lignocellulosic waste (LIG), were specifically selected; and two types of culturing media (M1 and M2) were used. After 6 months of sequential cultivation, lignocellulose (i.e., polysaccharides) degradation of enrichments M1 ...	2011	21869568
[Community acquired Stenotrophomonas maltophilia soft-tissue infection in a previously healthy patient.]		2011	21944651
characterization of copper-resistant rhizosphere bacteria from avena sativa and plantago lanceolata for copper bioreduction and biosorption.	copper is a toxic heavy metal widely used to microbial control especially in agriculture. consequently, high concentrations of copper residues remain in soils selecting copper-resistant organisms. in vineyards, copper is routinely used for fungi control. this work was undertaken to study copper resistance by rhizosphere microorganisms from two plants (avena sativa l. and plantago lanceolata l.) common in vineyard soils. eleven rhizosphere microorganisms were isolated, and four displayed high res ...	2011	22002857
detection of smqnr quinolone protection protein genes and its prevalence in clinical isolates of stenotrophomonas maltophilia in china.	to detect the novel variants of the smqnr family and analyze the prevalence of smqnr genes in clinical isolates of s. maltophilia in china. we collected 442 clinical isolates of s. maltophilia from 9 hospitals of four provinces in china. antimicrobial susceptibility test was performed for these isolates against six commonly used antibiotics (and combinations). the amplified sequences of smqnr genes using pcr were aligned with the known smqnr genes in genbank and smqnr database. the resistance ra ...	2011	22096133
biodiversity characterization of cellulolytic bacteria present on native chaco soil by comparison of ribosomal rna genes.	sequence analysis of the 16s ribosomal rna gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which actinobacteria, proteobacteria and acidobacteria accounted for more than 76% of all sequences. in both enriched samples, members of proteobacteria were the most frequently represented. the majority of ...	2011	22202170
molecular cloning of a copper-dependent laccase from the dye-decolorizing strain stenotrophomonas maltophilia aap56.	to clone the gene encoding the enzyme with laccase activity expressed by stenotrophomonas maltophilia aap56 and to construct an insertional mutation in that gene to determine its effect on dye decolourization and laccase activity in this strain.	2011	21973274
characterization in the archaeological excavation site of heterotrophic bacteria and fungi of deteriorated wall painting of herculaneum in italy.	microbiological characterization of frescos in four different locations (collegio degli augustali, casa del colonnato tuscanico, casa dello scheletro and casa del gran portale) of excavation sites of herculaneum was carried out. the use of infrared thermography allowed detecting sample points on frescos with greatest moisture not visible to the naked eye, resulting in structural damage. the microclimatic conditions provided perfect habitat for bacteria and fungi, particularly of spore forming an ...	2011	21882662
how could rapid bacterial identification improve the management of septic patients?		2011	21905779
the disinfecting potential of contact lens soutions used by sultan qaboos university students.	this study aimed to determine the disinfecting potential of some contact lens solutions used by some university students in oman.	2011	21969898
antibacterial and anti-biofilm effects of cathelicidin peptides against pathogens isolated from cystic fibrosis patients.	six different cathelicidin-derived peptides were compared to tobramycin for antibacterial and anti-biofilm effects against s. aureus, p. aeruginosa, and s. maltophilia strains isolated from cystic fibrosis patients. overall, smap-29, bmap-28, and bmap-27 showed relevant antibacterial activity (mic(50) 4-8μg/ml), and in some cases higher than tobramycin. in contrast, indolicidin, ll-37, and bac7(1-35) showed no significant antimicrobial activity (mic(50)>32μg/ml). killing kinetics experiments sho ...	2011	21849157
reducing the development of antibiotic resistance in critical care units.	bacteria becoming resistant to an increasing number of antibiotic classes are a major problem at hospitals including critical care units worldwide. awareness of this problem and the need to prevent the development of antibiotic resistance are very important, especially since very few new antibiotics will become available in the near future. this article gives an overview of the mechanisms of antibacterial resistance and actual resistance data worldwide of the most prevalent gram positive (mrsa, ...	2011	22188438
adhering ability of stenotrophomonas maltophilia is dependent on growth conditions.	the growth conditions are known to influence the bacterial adhesion to different kinds of surfaces. in the present study the adhering ability of stenotrophomonas maltophilia, on growth in nutrient rich media (tryptic soy broth (tsb)) and minimal media (luria bertani (lb)) was checked by viable cell count and spectrophotometric method. tsb grown s. maltophilia showed higher adhesion compared to bacteria grown in lb broth, to both biotic and abiotic surfaces. however, when bacteria were grown in l ...	2011	22073545
[distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse].	to investigate the relationship between microbial community and the blight diseases in rhizosphere of carnation for biological control.	2011	21866707
microbial biofilms on the sandstone monuments of the angkor wat complex, cambodia.	discoloring biofilms from cambodian temples angkor wat, preah khan, and the bayon and west prasat in angkor thom contained a microbial community dominated by coccoid cyanobacteria. molecular analysis identified chroococcidiopsis as major colonizer, but low similarity values (<95%) suggested a similar genus or species not present in the databases. in only two of the six sites sampled were filamentous cyanobacteria, microcoleus, leptolyngbya, and scytonema, found; the first two detected by sequenc ...	2011	22006074
polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.).	common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ...	2011	22159885
evaluation of microorganisms cultured from injured and repressed tissue regeneration sites in endangered giant aquatic ozark hellbender salamanders.	investigation into the causes underlying the rapid, global amphibian decline provides critical insight into the effects of changing ecosystems. hypothesized and confirmed links between amphibian declines, disease, and environmental changes are increasingly represented in published literature. however, there are few long-term amphibian studies that include data on population size, abnormality/injury rates, disease, and habitat variables to adequately assess changes through time. we cultured and i ...	2011	22205979
stenotrophomonas interspecies differentiation and identification by gyrb sequence analysis.	stenotrophomonas species are found commonly in environmental and clinical samples; stenotrophomonas maltophilia is an important opportunistic pathogen of humans. traditional phenotyping protocols, as well as genotyping by 16s rrna gene sequence analysis, do not reliably distinguish the species of stenotrophomonas. sequence analyses of two targeted pcr-amplified regions of the gyrb gene, which encodes the β-subunit of dna gyrase, enabled resolution and identification of these species. most type s ...	2011	22092789
Bacterial cis-2-unsaturated fatty acids found in the cystic fibrosis airway modulate virulence and persistence of Pseudomonas aeruginosa.	There is an increasing appreciation of the polymicrobial nature of many bacterial infections such as those associated with cystic fibrosis (CF) and of the potentially important role for interspecies interactions in influencing both bacterial virulence and response to therapy. Patients with CF are often co-infected with Pseudomonas aeruginosa and other pathogens including Burkholderia cenocepacia and Stenotrophomonas maltophilia. These latter bacteria produce signal molecules of the diffusible si ...	2011	22134647
volatiles of two growth-inhibiting rhizobacteria commonly enroll atwrky18 function.	interactions with the (a)biotic environment play key roles for plant's fitness and vitality. in addition to direct surface-to-surface contact, volatile chemicals also affect other organisms. volatiles of serratia plymuthica and stenotrophomonas maltophilia significantly inhibited growth and induced h(2) o(2) production in arabidopsis in dual-culture. within one day, transcriptional changes could be observed by promoter-gus assays using a stress inducible, w-box containing 4xgst1 construct. expr ...	2011	22188129
antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria).	the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ...	2011	22095134
Frequency of oral mucositis and microbiological analysis in children with acute lymphoblastic leukemia treated with 0.12% chlorhexidine gluconate.	In view of the morbidity potential of oral complications in patients with leukemia, this study evaluated the clinical and microbiological alterations that occur in the oral mucosa of children with acute lymphoblastic leukemia (ALL) undergoing antineoplastic chemotherapy and prophylactic administration of 0.12% chlorhexidine gluconate. The sample consisted of 17 children aged 2 to 12 years that underwent clinical examination of the oral mucosa for the detection of oral lesions. In addition, biolo ...	2011	21861031
trends in the susceptibility of clinically important resistant bacteria to tigecycline: results from the tigecycline in-vitro surveillance in taiwan (tist), 2006-2010.	the tigecycline in-vitro surveillance in taiwan (tist), a nationwide, prospective surveillance during 2006 to 2010, collected a total of 7,793 clinical isolates, including methicillin-resistant staphylococcus aureus (mrsa) (n=1,834), penicillin-resistant streptococcus pneumoniae (prsp) (n=423), vancomycin-resistant enterococci (vre) (n=219), extended-spectrum β-lactamase (esbl)-producing escherichia coli (n=1,141), esbl-producing klebsiella pneumoniae (n=1,330), acinetobacter baumannii (n=1,645) ...	2011	22203598
biodegradation potential of oily sludge by pure and mixed bacterial cultures.	the biodegradation capacity of aliphatic and aromatic hydrocarbons of petrochemical oily sludge in liquid medium by a bacterial consortium and five pure bacterial cultures was analyzed. three bacteria isolated from petrochemical oily sludge, identified as stenotrophomonas acidaminiphila, bacillus megaterium and bacillus cibi, and two bacteria isolated from a soil contaminated by petrochemical waste, identified as pseudomonas aeruginosa and bacillus cereus demonstrated efficiency in oily sludge d ...	2011	21993328
a multicenter surveillance of antimicrobial resistance on stenotrophomonas maltophilia in taiwan.	background: stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in debilitated hosts. clinical management of s. maltophilia is challenging due to its intrinsic resistance to a variety of antibiotics. this study investigated the trend and prevalence of antimicrobial resistance in s. maltophilia from a nationwide surveillance study in taiwan. methods: s. maltophilia isolates were collected biennially between 1998 and 2008 as part of the taiwan surveillance of antimicrob ...	2011	22154599
functional characterization of an nadph dependent 2-alkyl-3-ketoalkanoic acid reductase involved in olefin biosynthesis in stenotrophomonas maltophilia.	oled is shown to play a key reductive role in the generation of alkenes (olefins) from acyl thioesters in stenotrophomonas maltophilia. the gene coding for oled clusters with three other genes, oleabc, and all appear to be transcribed in the same direction as an operon in various olefin producing bacteria. in this study, a series of substrates varying in chain length and stereochemistry were synthesized and used to elucidate the functional role and substrate specificity of oled. we demonstrated ...	2011	21958090
removal of high concentrations of nh(3) by a combined photoreactor and biotrickling filter system.	average emission levels as high as 800 ppm(v) nh(3) have often been found during the anaerobic fermentation process. at these levels, nh(3) is regarded as an environmental toxic compound. high concentrations of nh(3) gas are difficult to treat in a single treatment process, suggesting that, in terms of economic cost and treatment performance, a coupled system may be a feasible technological alternative. in the coupled tio(2) photocatalytic-biological treatment system evaluated here, the optimal ...	2011	22175871
Identification and characterization of a chitinase of Stenotrophomonas maltophilia, a bacterium that is antagonistic towards fungal phytopathogens.	A rhizosphere strain of Stenotrophomonas maltophilia strain MUJ that is strongly antagonistic towards fungal phytopathogens secretes to the culture medium a single form of active chitinolytic enzyme belonging to family 18 of glycosyl hydrolases. The chitinase was purified by a two-stage procedure embracing fractionation with ammonium sulfate and affinity chromatography. The molecular mass of the purified enzyme determined by SDS-PAGE was approximately 52kDa. The enzyme demonstrated highest activ ...	2011	21945415
[Observation by transmission electron microscope and identification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus].	The pine wood nematode, Bursaphlenchus xylophilus, morphologically similar to B. mucronatus, is the pathogen of pine wilt disease. This study was focused on the endophytic bacteria present in these nematodes.	2011	22097772
hessian fly-associated bacteria: transmission, essentiality, and composition.	plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ...	2011	21858016
effect of surfactants on pah biodegradation by a bacterial consortium and on the dynamics of the bacterial community during the process.	the aim of this work was to evaluate the effect of a non-biodegradable (tergitol np-10) and a biodegradable (tween-80) surfactant on growth, degradation rate and microbial dynamics of a polycyclic aromatic hydrocarbon (pahs) degrading consortium (c2pl05) from a petroleum polluted soil, applying cultivable and non cultivable techniques. growth and degradation rate were significantly lower with tergitol np-10 than that with tween-80. toxicity did not show any significant reduction with tergitol np ...	2011	21862321
rapid genotyping of achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia isolates using melting curve analysis of rapd-generated dna fragments (mcrapd).	typing of bacteria is important for monitoring newly emerging pathogens and for examining local outbreaks. we evaluated the randomly amplified polymorphic dna technique in combination with melting curve analysis (mcrapd) of the amplified dna fragments to genotype isolates from five gram-negative species, i.e. achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia. by determining the melting temperature peaks of the ampl ...	2011	21320595
isolation of a selected microbial consortium capable of degrading methyl parathion and p-nitrophenol from a contaminated soil site.	a bacterial consortium with the ability to degrade methyl parathion and p-nitrophenol, using these compounds as the only carbon source, was obtained by selective enrichment in a medium with methyl parathion. samples were taken from moravia, medellin; an area that is highly contaminated, owing to the fact that it was used as a garbage dump from 1974 to 1982. acinetobacter sp, pseudomonas putida, bacillus sp, pseudomonas aeruginosa citrobacter freundii, stenotrophomonas sp, flavobacterium sp, prot ...	2011	21328125
long term performance of an arsenite-oxidizing-chlorate-reducing microbial consortium in an upflow anaerobic sludge bed (uasb) bioreactor.	a chlorate (clo(3)(-)) reducing microbial consortium oxidized arsenite (as(iii)) to arsenate (as(v)) in an upflow anaerobic sludge-bed bioreactor over 550 days operation. as(iii) was converted with high conversion efficiencies (>98%) at volumetric loadings ranging from 0.45 to 1.92 mmol as/(l(reactor)d). the oxidation of as(iii) was linked to the complete reduction of clo(3)(-) to cl(-) and h(2)o, as demonstrated by a molar ratio of approximately 3.0 mol as(iii) oxidized per mole of cl(-) formed ...	2011	21333531
screening and identification of a novel esterase estpe from a metagenomic dna library.	esterases represent a large family of hydrolases with broad substrate specificity and functional sequence space. although many attempts to screen new esterases have been conducted, there have been few reports conducted to discriminate unique enzymes from typical ones based on novel structure and function. in this study, we discovered an esterase and a novel family through a successive assay of whole cells and crude lysates (oxidative open condition). the screened putative esterases from the meta ...	2011	21369973
efficacy of an anaerobic swab transport system to maintain aerobic and anaerobic microorganism viability after storage at -80 degrees c.	an amies agar gel swab transport system was evaluated for its ability to maintain bacterial viability and relative quantity after freezing at -80°c. nine american type culture collection (atcc) bacterial strains were used: 3 anaerobic strains (propionibacterium acnes, peptostreptococcus anaerobius, and clostridium sporogenes) and 6 facultative or strict aerobic bacterial strains (stenotrophomonas maltophilia, escherichia coli ([atcc 25922 and atcc 11775], salmonella enterica subsp. enterica sero ...	2011	21217035
secretion, modification, and regulation of ax21.	innate immunity provides a first line of defense against pathogen attack and is activated rapidly following infection. although it is now widely appreciated that host receptors of conserved microbial signatures play a key role in innate immunity in plants and animals, very little is known about the biological function of the microbially derived molecules recognized by such receptors. we have recently demonstrated that the rice xa21 receptor binds the axy(s)22 peptide corresponding to the n-termi ...	2011	21236725
in vitro and in vivo properties of bal30376, a β-lactam and dual beta-lactamase inhibitor combination with enhanced activity against gram-negative bacilli that express multiple β-lactamases.	bal30376 is a triple combination comprising a siderophore monobactam, bal19764; a novel bridged monobactam, bal29880, which specifically inhibits class c β-lactamases; and clavulanic acid, which inhibits many class a and some class d β-lactamases. the mic(90) was ≤ 4 μg/ml (expressed as the concentration of bal19764) for most species of the enterobacteriaceae family, including strains that produced metallo-β-lactamases and were resistant to all of the other β-lactams tested. the mic(90) for sten ...	2011	21245441
delineation of stenotrophomonas spp. by multi-locus sequence analysis and maldi-tof mass spectrometry.	the genus stenotrophomonas is genetically and phenotypically heterogeneous. of the nine species now accepted, only s. maltophilia is of clinical importance. based on dna-sequences of seven house keeping genes, it encompasses genogroups of dna-similarity below 97% that predominantly comprise strains of environmental origin. therefore, in order to unravel the uneven distribution of environmental isolates within genogroups and reveal genetic relationships within the genus, there is need for an easy ...	2011	21247714
influence of referral bias on the clinical characteristics of patients with gram-negative bloodstream infection.	summaryreferral bias can influence the results of studies performed at tertiary-care centres. in this study, we evaluated demographic and microbiological factors that influenced referral of patients with gram-negative bloodstream infection (bsi). we identified 2919 and 846 unique patients with gram-negative bsi in a referral cohort of patients treated at mayo clinic hospitals and a population-based cohort of olmsted county, minnesota, residents between 1 january 1998 and 31 december 2007, respec ...	2011	21281552
antithymocyte globulin-induced acute lung injury during transplantation for aplastic anemia.	a 10-year-old boy with acquired, very severe aplastic anemia developed acute lung injury after the administration of equine antithymocyte globulin, during conditioning for allogenic bone marrow transplantation. limited cases of antithymocyte globulin-induced acute lung injury have been described in adults. the respiratory worsening was sudden and required mechanical ventilation. the clinical course was complicated by sepsis with escherichia coli, vancomycin-resistant enterococci, and stenotropho ...	2011	21285902
count, identification and antimicrobial susceptibility of bacteria recovered from dental solid waste in brazil.	in brazil, few studies on microbial content of dental solid waste and its antibiotic susceptibility are available. an effort has been made through this study to evaluate the hazardous status of dental solid waste, keeping in mind its possible role in cross-infection chain. six samples of solid waste were collected at different times and seasons from three dental health services. the microbial content was evaluated in different culture media and atmospheric conditions, and the isolates were submi ...	2011	21288707
stenotrophomonas maltophilia resistance to trimethoprim/sulfamethoxazole mediated by acquisition of sul and dfra genes in a plasmid-mediated class 1 integron.	stenotrophomonas maltophilia is becoming a more and more common cause of infections. in this study, the minimal inhibitory concentrations of trimethoprim/sulfamethoxazole (sxt), ceftazidime, minocycline, levofloxacin, chloramphenicol and ticarcillin/clavulanic acid were determined and the distribution of integrons and sul1, sul2 and dfra genes was investigated in 102 s. maltophilia isolates collected from patients treated in 31 hospitals in anhui, china, in the month of september in 2006-2008. t ...	2011	21296557
a cluster of acute-onset postoperative endophthalmitis over a 1-month period: investigation of an outbreak caused by uncommon species.	to report the clinical course, treatment response and prognosis of eight cases which developed acute-onset postoperative endophthalmitis over a 1-month period.	2011	20733020
late-onset intrascleral dissemination of stenotrophomonas maltophilia scleritis after pterygium excision.	to report a case of pterygium excision-related infectious scleritis with late-onset intrascleral dissemination.	2011	21173698
biocidal efficacy of silver-impregnated contact lens storage cases in vitro.	silver-impregnated contact lens (cl) storage cases are designed to reduce microbial contamination during use, but there are limited data on their effectiveness. this study evaluated early antimicrobial activity of silver-impregnated cl cases and silver-release characteristics in vitro.	2011	20720221
conversion of oleic acid to 10-hydroxystearic acid by whole cells of stenotrophomonas nitritireducens.	the optimal reaction conditions for the conversion of oleic acid to 10-hydroxystearic acid by whole cells of stenotrophomonas nitritireducens were: ph 7.5, 35 °c, 0.05% (w/v) tween 80, 20 g cells l(-1), and 30 g oleic acid l(-1) in an anaerobic atmosphere. under these conditions, the cells produced 31.5 g 10-hydroxystearic acid l(-1) over 4 h with a conversion yield of 100% (mol/mol) and a productivity of 7.9 g l(-1) h(-1), indicating that oleic acid was converted completely to 10-hydroxystearic ...	2011	21207107
decreased mucosal oxygen tension in the maxillary sinuses in patients with cystic fibrosis.	pseudomonas aeruginosa in the sinuses plays a role in the lungs in cystic fibrosis (cf) patients, but little is known about the sinus environment where the bacteria adapt. anoxic areas are found in the lower respiratory airways but it is unknown if the same conditions exist in the sinuses.	2011	21208831
stenotrophomonas maltophilia causing early onset neonatal sepsis.	stenotrophomonas maltophilia, a multi drug resistant non fermenting gram negative bacillus is an increasingly common nosocomial pathogen, especially in intensive care units. comparatively few cases of infection have been reported in neonatal population. we present two cases of early onset neonatal sepsis due to s.maltophilia and a brief review of documented isolation in neonates.	2011	21654005
a comparative study of biodegradation of vinyl acetate by environmental strains.	four gram-negative strains, e3_2001, ec1_2004, ec3_3502 and ec2_3502, previously isolated from soil samples, were subjected to comparative studies in order to select the best vinyl acetate degrader for waste gas treatment. comparison of biochemical and physiological tests as well as the results of fatty acids analyses were comparable with the results of 16s rrna gene sequence analyses. the isolated strains were identified as pseudomonas putida ec3_2001, pseudomonas putida ec1_2004, achromobacter ...	2011	21654921
fatal hemorrhagic pneumonia caused by stenotrophomanas maltophilia in a patient with non-hodgkin lymphoma.	stenotrophomonas maltophilia is increasingly emerging as a multiresistant pathogen in the hospital environment. in immunosuppressed patients, this bacterium may cause severe infections associated with sepsis and multiple organ dysfunction. we report on a 57-year-old woman treated with intensive chemotherapy for non-hodgkin lymphoma who developed severe neutropenia, hemorrhagic pneumonia, and acute respiratory failure, which led to her death within 36 h of onset of pneumonia. postmortem examinati ...	2011	21656194
culture-dependent and -independent molecular analysis of the bacterial community within uranium ore.	the bacterial community structure within a uranium ore was investigated using culture-dependent and -independent clone library analysis and denaturing gradient gel electrophoresis of 16s rrna genes. the major aerobic heterotrophic bacteria were isolated and identified, and their resistance to uranium and other heavy metals was characterized. together with near neutral ph, moderate organic carbon content, elevated u and other heavy metals (v, ni, mn, cu, etc.), the ore showed high microbial count ...	2011	21656801
diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis.	microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ...	2011	21660545
antimicrobial susceptibilities of commonly encountered bacterial isolates to fosfomycin determined by agar dilution and disk diffusion methods.	we studied the antimicrobial activity of fosfomycin against 960 strains of commonly encountered bacteria associated with urinary tract infection using standard agar dilution and disk diffusion methods. species studied included 3 common species of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia; methicillin-susceptible and -resistant staphylococcus aureus; and vancomycin-susceptible and resistant enterococcus faecalis and e. faecium. mics and ...	2011	21670185
use of the diversilab repetitive sequence-based polymerase chain reaction system for genotyping stenotrophomonas maltophilia isolates in different wards of one hospital.		2011	21802174
emerging gram-negative infections in burn wounds.	gram-negative infection remains a major contributor to morbidity, mortality, and cost of care. in the absence of comparative multinational epidemiological studies specific to burn patients, we sought to review literature trends in emerging gram-negative burn wound infections within the past 60 years. mapping trends in these organisms, although in a minority compared with the six "eskape" pathogens currently being targeted by the infectious diseases society of north america, would identify pathog ...	2011	21792068
biodegradation of geosmin in drinking water by novel bacteria isolated from biologically active carbon.	three strains of gram-negative bacteria capable of removing geosmin from drinking water were isolated from biologically active carbon and identified to be chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp. based on physio-biochemistry analysis and 16s rrna gene sequence analysis. removal efficiencies of 2 mg/l geosmin in mineral salts medium were 84.0%, 80.2% and 74.4% for chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp., respectively, while removal efficiencies of ...	2011	21790055
xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.	the xenopus laevis oocyte has been the workhorse for the investigation of ion transport proteins. these large cells have spawned a multitude of novel techniques that are unfathomable in mammalian cells, yet the fickleness of the oocyte has driven many researchers to use other membrane protein expression systems. here, we show that some colonies of xenopus laevis are infected with three multi-drug-resistant bacteria: pseudomonas fluorescens, pseudomonas putida, and stenotrophomonas maltophilia. o ...	2011	21788613
an unusual cause of continuous ambulatory peritoneal dialysis-associated outpatient peritonitis: stenotrophomonas maltophilia.		2011	21782283
stevens-johnson syndrome in a juvenile systemic lupus erythematosus patient.	stevens-johnson syndrome (sjs) is a severe and rare immune-mediated cutaneous reaction usually induced by drugs or infections. few case reports have demonstrated sjs associated with adult systemic lupus erythematosus (sle), and rarely in juvenile sle (jsle) patients. however, to the best of our knowledge the prevalence of this life-threatening cutaneous disease in the pediatric lupus population has not been studied. therefore, from january 1983 to december 2010, 5508 patients were followed-up at ...	2011	21768172
stenotrophomonas maltophilia in cystic fibrosis: improved detection by the use of selective agar and evaluation of antimicrobial resistance.	background: we implemented a selective medium for improved detection of stenotrophomonas maltophilia in sputum samples from cf patients. we also performed antimicrobial susceptibility testing with eight antibiotics. methods: a total of 623 consecutive sputum samples from 165 cf patients in a german cf center were cultured onto conventional media and onto steno medium agar (sma). all isolates confirmed as s. maltophilia by biochemical and molecular methods were subjected to antimicrobial suscepti ...	2011	21763218
c-s targeted biodegradation of dibenzothiophene by stenotrophomonas sp. nisoc-04.	crude oil-contaminated soil samples were gathered across khuzestan oilfields (national iranian south oil company, nisoc) consequently experienced a screening procedure for isolating c-s targeted dibenzothiophene-biodegrading microorganisms with previously optimized techniques. among the isolates, a bacterial strain was selected due to its capability of biodegrading dibenzothiophene in a c-s targeted manner in aqueous phases and medium mostly consisting of separately biphasic water-gasoline. the ...	2011	21750993
infection in immunocompromised hosts and the organ transplant recipient -essentials.		2011	21748845
acceleration of nonylphenol and 4-tert-octylphenol degradation in sediment by phragmites australis and associated rhizosphere bacteria.	we investigated biodegradation of technical nonylphenol (tnp) in phragmites australis rhizosphere sediment by conducting degradation experiments using sediments spiked with tnp. accelerated tnp removal was observed in p. australis rhizosphere sediment, whereas tnp persisted in unvegetated sediment without plants and in autoclaved sediment with sterile plants, suggesting that the accelerated tnp removal resulted largely from tnp biodegradation by rhizosphere bacteria. three bacterial strains, ste ...	2011	21736332
ciprofloxacin in critically ill children.	data regarding the use of fluoroquinolones in critically ill children are scarce. we present our experience regarding the use of ciprofloxacin in this specific patient population. we prospectively identified all paediatric patients who received ciprofloxacin treatment in the intensive care unit of the tertiary care p. & a. kyriakou children's hospital during a three year period (2005 to 2008). eighteen paediatric patients (mean age 23 months, 12 females) who received intravenous ciprofloxacin we ...	2011	21823382
diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze).	the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ...	2011	21715960
phylogenetic analysis of culturable marine bacteria in sediments from the south korean yellow sea.	biogeochemical and microbiological characterization of marine sediments taken from the yellow sea of south korea was carried out. one hundred and thirty six bacterial strains were isolated, characterized and phylogenetic relationship was evaluated. the gene sequences of 16s rdna regions were examined to study the phylogenetic analysis of bacterial community in the marine sediments. among 136 isolates, 5 bacterial isolates were identified as novel members, remaining 131 isolates were fall into 5 ...	2011	21675219
inactivation of mrca gene derepresses the basal-level expression of l1 and l2 {beta}-lactamases in stenotrophomonas maltophilia.	objectives to characterize the relationship between inactivation of the mrca gene and +¦-lactamase expression and +¦-lactams resistance in stenotrophomonas maltophilia kj and to investigate the involvement of ampr, ampn-ampg, ampd(i) and crebc in this. methods the mrca deletion mutant kj+ömrca was constructed to investigate the role of this putative penicillin-binding protein 1a (pbp1a) in +¦-lactamase expression and +¦-lactam resistance. the +öampr, +öampng, +öampdi and +öcrebc alleles were int ...	2011	21719470
phenotypic and genotypic characterization of stenotrophomonas maltophilia isolates from patients with cystic fibrosis: genome diversity, biofilm formation, and virulence.	abstract:	2011	21729271
nile red detection of bacterial hydrocarbons and ketones in a high-throughput format.	a method for use in high-throughput screening of bacteria for the production of long-chain hydrocarbons and ketones by monitoring fluorescent light emission in the presence of nile red is described. nile red has previously been used to screen for polyhydroxybutyrate (phb) and fatty acid esters, but this is the first report of screening for recombinant bacteria making hydrocarbons or ketones. the microtiter plate assay was evaluated using wild-type and recombinant strains of shewanella oneidensis ...	2011	21712420
genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere.	the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ...	2011	21686169
testosterone-mineralizing culture enriched from swine manure: characterization of degradation pathways and microbial community composition.	environmental releases and fate of steroid sex hormones from livestock and wastewater treatment plants are of increasing regulatory concern. despite the detection of these hormones in manures, biosolids, and the environment, little attention has been paid to characterization of fecal bacteria capable of hormone degradation. the enrichments of (swine) manure-borne bacteria capable of aerobic testosterone degradation were prepared and the testosterone mineralization pathway was elucidated. six dna ...	2011	21740029
identification of stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure.	aims:ôçé aim of the study is to identify accurately stenotrophomonas maltophilia isolates recovered from environmental and clinical samples. methods and results:ôçé recovery of sten.-ámaltophilia-like isolates from soil samples using the vancomycin, imipenem, amphotericin b (via) selective agar medium enabled distinction of various morphotype colonies. a set of soil and clinical isolates was tested for species identification using different methods. 16s rdna analyses showed the dark green with a ...	2011	21819497
resolving the phylogenetic and taxonomic relationship of xanthomonas and stenotrophomonas strains using complete rpob gene sequence.		2011	21686310
arsenic-resistant bacteria solubilized arsenic in the growth media and increased growth of arsenic hyperaccumulator pteris vittata l.	the role of arsenic-resistant bacteria (arb) in arsenic solubilization from growth media and growth enhancement of arsenic-hyperaccumulator pteris vittata l. was examined. seven arb (tolerant to 10mm arsenate) were isolated from the p. vittata rhizosphere and identified by 16s rrna sequencing as pseudomonas sp., comamonas sp. and stenotrophomonas sp. during 7-d hydroponic experiments, these bacteria effectively solubilized arsenic from the growth media spiked with insoluble feaso(4) and alaso(4) ...	2011	21840210
mucosal surveillance cultures in predicting gram-negative late-onset sepsis in neonatal intensive care units.	this study aimed to examine the spectrum and time course of gut and nasopharyngeal colonization with gram-negative micro-organisms, and to define the value of surveillance cultures in predicting late-onset sepsis in neonates admitted to neonatal intensive care units. nasopharyngeal and rectal swabs were collected on admission and twice weekly thereafter in 278 neonates admitted within the first 72-áh of life with risk factors of early-onset sepsis. sterile body fluid cultures were obtained on ad ...	2011	21684633
an mlsa-based online scheme for the rapid identification of stenotrophomonas isolates.	an online scheme to assign stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (mlsa), which is based on the dna sequencing of selected fragments of the housekeeping genes atp synthase alpha subunit (atpa), the recombination repair protein (reca), the rna polymerase alpha subunit (rpoa) and the excision repair beta subunit (uvrb). this mlsa-based scheme was validated using eight of the 10 stenotrophomonas species that have been previously described. t ...	2011	21739025
the binding of triclosan to smet, the repressor of the multidrug efflux pump smedef, induces antibiotic resistance in stenotrophomonas maltophilia.	the wide utilization of biocides poses a concern on the impact of these compounds on natural bacterial populations. furthermore, it has been demonstrated that biocides can select, at least in laboratory experiments, antibiotic resistant bacteria. this situation has raised concerns, not just on scientists and clinicians, but also on regulatory agencies, which are demanding studies on the impact that the utilization of biocides may have on the development on resistance and consequently on the trea ...	2011	21738470
Low Frequency of Endemic Patient-to-Patient Transmission of Antibiotic-Resistant Gram-Negative Bacilli in a Pediatric Intensive Care Unit.	We sought to determine the frequency of horizontal transmission of antibiotic-resistant gram-negative bacilli (ARGNB) in a pediatric intensive care unit during a nonoutbreak period. Among 5,300 admissions over 39 consecutive months, 13 ARGNB clusters involving 35 children were identified by pulsed-filed gel electrophoresis analysis, which suggests that person-to-person transmission was uncommon.	2011	21828974