Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| dissecting malaria biology and epidemiology using population genetics and genomics. | molecular approaches have an increasingly recognized utility in surveillance of malaria parasite populations, not only in defining prevalence and incidence with higher sensitivity than traditional methods, but also in monitoring local and regional parasite transmission patterns. in this review, we provide an overview of population genetic and genomic studies of human-infecting plasmodium species, highlighting recent advances in the field. in accordance with the renewed impetus for malaria eradic ... | 2017 | 27825828 |
| molecular characterization and phylogenetic analysis of plasmodium vivax, plasmodium falciparum, plasmodium ovale, plasmodium malariae and plasmodium cynomolgi. | 18s ribosomal rna gene sequences of different species of plasmodium were aligned and analyzed to determine the molecular diversity among different species of plasmodium. at content of p. cynomolgi, p. ovale, p. falciparum, p. vivax and p. malariae ranged from 62.30 to 63.15, 63.90 to 65.29, 66.67 to 68.40, 61.66 to 63.25 and 64.09 to 76.36 in case respectively. gc content of p. cynomolgi, p. ovale, p. falciparum, p. vivaxand p. malariae ranged from 36.85 to 37.70, 34.71 to 36.43, 31.60 to 33.27, ... | 2017 | 28316417 |
| evidence of non-plasmodium falciparum malaria infection in kédougou, sénégal. | expanded malaria control efforts in sénégal have resulted in increased use of rapid diagnostic tests (rdt) to identify the primary disease-causing plasmodium species, plasmodium falciparum. however, the type of rdt utilized in sénégal does not detect other malaria-causing species such as plasmodium ovale spp., plasmodium malariae, or plasmodium vivax. consequently, there is a lack of information about the frequency and types of malaria infections occurring in sénégal. this study set out to bette ... | 2017 | 28049489 |
| severe malaria complicated by g6pd deficiency in a pediatric tanzanian immigrant. | approximately 1,500 cases of malaria are diagnosed in the united states each year. most cases are travelers and immigrants returning from parts of the world where malaria transmission occurs. malaria is the most frequent cause of systemic febrile illness without localizing symptoms in travelers returning from the developing world, so vigilance by providers is needed when evaluating patients returning from areas in which malaria is endemic. despite the availability of effective treatment, malaria ... | 2017 | 25762879 |
| lamp kit for diagnosis of non-falciparum malaria in plasmodium ovale infected patients. | microscopy and rapid diagnosis tests have a limited sensitivity in diagnosis of malaria by plasmodium ovale. the lamp kit (loopamp®) can be used in the field without special equipment and could have an important role in malaria control programmes in endemic areas and for malaria diagnosis in returned travellers. the performance of the pan primer of the kit in detecting malaria by p. ovale was compared with the results of standard npcr in samples of patients returning from p. ovale endemic areas. | 2017 | 28061871 |
| malaria in the guangxi zhuang autonomous region in china: a twelve-year surveillance data study. | the incidence of an indigenous malaria, defined as malaria acquired by a local mosquito transmission, declined from 2004 to 2015 in the guangxi zhuang autonomous region. however, imported malaria, defined as malaria acquired from other endemic regions outside of china, has been increasing in the region, as in the rest of the country, particularly the disease caused by plasmodium falciparum. a retrospective study was conducted to explore malaria-endemic characteristics in guangxi during the 2004- ... | 2017 | 28820683 |
| cryptic plasmodium ovale concurrent with mixed plasmodium falciparum and plasmodium malariae infection in two children from central african republic. | since several malaria parasite species are usually present in a particular area, co-infections with more than one species of plasmodium are more likely to occur in humans infected in these areas. in many mixed infections, parasite densities of the cryptic species may be low and often not recognized in clinical practice. | 2017 | 28810865 |
| non-falciparum malaria imported mainly from africa: a review from a portuguese hospital. | non-falciparum malaria (nfm) has been reported to be responsible for around 25% of imported malaria cases in europe but is often neglected due to its less severe clinical course when compared to plasmodium falciparum. differentiation between species is however crucial for a correct approach. the objective of this study is to report the cases of this often missed aetiology of malaria in a tertiary hospital in portugal. | 2017 | 28743266 |
| a dual colour fluorescence in situ hybridization (fish) assay for identifying the zoonotic malaria parasite plasmodium knowlesi with a potential application for the specific diagnosis of knowlesi malaria in peripheral-level laboratories of southeast asia. | plasmodium knowlesi is primarily responsible for zoonotic malaria in several southeast asian countries. precise identification of the parasite in the blood of patients presently relies on an expensive and elaborate pcr procedure because microscopic examination of blood and other available field identification techniques lack adequate specificity. therefore, the use of a simple and inexpensive dual-colour fluorescence in situ hybridization (fish) assay, analogous to fish assays recently described ... | 2017 | 28724402 |
| [biological disturbances affecting people with malaria: about thirty cases]. | 2017 | 28674567 | |
| imported malaria in pregnant women experienced in japan. | with ever-growing global exchanges, the number of travelers, including pregnant women, to the tropics is increasing, which poses a risk of contracting malaria. although there are several reports on imported malaria in pregnancy from western countries, those focusing on cases experienced in japan are very limited. | 2017 | 28583709 |
| malaria pathogenesis. | in the mosquito-human life cycle, the six species of malaria parasites infecting humans (plasmodium falciparum, plasmodium vivax, plasmodium ovale wallickeri, plasmodium ovale curtisi, plasmodium malariae, and plasmodium knowlesi) undergo 10 or more morphological states, replicate from single to 10,000+ cells, and vary in total population from one to many more than 10(6) organisms. in the human host, only a small number of these morphological stages lead to clinical disease and the vast majority ... | 2017 | 28533315 |
| evaluation of the utility value of three diagnostic methods in the detection of malaria parasites in endemic area. | malaria is a debilitating disease with high morbidity and mortality in africa, commonly caused by different species of the genus plasmodium in humans. misdiagnosis is a major challenge in endemic areas because of other disease complications and technical expertise of the medical laboratory staff. microscopic method using giemsa-stained blood film has been the mainstay of diagnosis of malaria. however, since 1993 when rapid diagnostic test (rdt) kits were introduced, they have proved to be effect ... | 2017 | 28477621 |
| nested multiplex pcr for identification and detection of human plasmodium species including plasmodium knowlesi. | to develop a new technique for diagnosis of plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of plasmodium causing human malaria. | 2017 | 28442114 |
| metal-dna interactions improve signal in high-resolution melting of dna for species differentiation of plasmodium parasite. | the success of high-resolution melting (hrm) analysis for distinguishing similar dnas with minor base mismatch differences is limited. here, metal-mediated structural change in dna has been exploited to amplify hrm signals leading to differentiation of target dnas in an orthologous gene corresponding to four plasmodium species. conserved 26-mer ssdnas from ldh gene of the four plasmodium species were employed as targets. a capture probe (cp) that is fully complementary to the plasmodium falcipar ... | 2017 | 28421327 |
| [role of primaquine in malaria control and elimination in french-speaking africa]. | primaquine, an 8-aminoquinoline, is a relatively unknown and underutilized drug in french-speaking african countries. it acts against the liver stage parasites of all human malaria species, asexual blood stages of plasmodium vivax and, to a lesser degree, plasmodium falciparum; p. falciparum mature gametocytes, and p. vivax and plasmodium ovale hypnozoites. gastrointestinal disturbances are its most common side effects. the clinical utility of primaquine is limited due to its hematological side ... | 2017 | 28417346 |
| molecular diagnosis of plasmodium ovale by photo-induced electron transfer fluorogenic primers: pet-pcr. | accurate diagnosis of malaria infections continues to be challenging and elusive, especially in the detection of submicroscopic infections. developing new malaria diagnostic tools that are sensitive enough to detect low-level infections, user friendly, cost effective and capable of performing large scale diagnosis, remains critical. we have designed novel self-quenching photo-induced electron transfer (pet) fluorogenic primers for the detection of p. ovale by real-time pcr. in our study, a total ... | 2017 | 28640824 |
| molecular and immunological analyses of confirmed plasmodium vivax relapse episodes. | relapse infections resulting from the activation hypnozoites produced by plasmodium vivax and plasmodium ovale represent an important obstacle to the successful control of these species. a single licensed drug, primaquine is available to eliminate these liver dormant forms. to date, investigations of vivax relapse infections have been few in number. | 2017 | 28558712 |
| [spontaneous spleen rupture with p. ovale malaria]. | 2017 | 28545674 | |
| case report of plasmodium ovale curtisi malaria in sri lanka: relevance for the maintenance of elimination status. | following its recent certification as malaria-free, imported infections now pose the greatest threat for maintaining this status in sri lanka. imported infections may also introduce species that are uncommon or not previously endemic to these areas. we highlight in this case report the increasing importance of less common malaria species such as plasmodium ovale in elimination settings and discuss its relevance for the risk of malaria resurgence in the country. | 2017 | 28438137 |
| a systematic review of the clinical presentation, treatment and relapse characteristics of human plasmodium ovale malaria. | despite increased efforts to control and ultimately eradicate human malaria, plasmodium ovale malaria is for the most part outside the focus of research or public health programmes. importantly, the understanding of p. ovale-nowadays regarded as the two distinct species p. ovale wallikeri and p. ovale curtisi-largely stems from case reports and case series lacking study designs providing high quality evidence. consecutively, there is a lack of systematic evaluation of the clinical presentation, ... | 2017 | 28284211 |
| dat-positive plasmodium ovale malaria presenting in a child with sickle cell anemia. | 2017 | 28211104 | |
| two sympatric types of plasmodium ovale and discrimination by molecular methods. | plasmodium ovale is widely distributed in tropical countries, whereas it has not been reported in the americas. it is not a problem globally because it is rarely detected by microscopy owing to low parasite density, which is a feature of clinical ovale malaria. p.o. curtisi and p.o. wallikeri are widespread in both africa and asia, and were known to be sympatric in many african countries and in southeast asian countries. small subunit ribosomal rna (ssurrna) gene, cytochrome b (cytb) gene, and m ... | 2016 | 28065415 |
| parasitological correlates of plasmodium ovale curtisi and plasmodium ovale wallikeri infection. | malaria, due to plasmodium ovale, can be challenging to diagnose due to clinically mild disease and low parasite burden. two genetically distinct sub-species of p. ovale exist: plasmodium ovale curtisi (classic) and plasmodium ovale wallikeri (variant). it is presently unknown if the sub-species causing infection affects performance of malaria diagnostic tests. the aim of this work was to understand how the genetically distinct sub-species, p. o. curtisi and p. o. wallikeri, affect malaria diagn ... | 2016 | 27832785 |
| [epidemiologic characteristics of plasmodium ovale in china in 2011-2014]. | to investigate the epidemiological characteristics of plasmodium ovale in 2011-2014 for malaria control policy making during the phase of malaria elimination in china. | 2016 | 27029361 |
| assessment of point-of-care diagnostics for g6pd deficiency in malaria endemic rural eastern indonesia. | patients infected by plasmodium vivax or plasmodium ovale suffer repeated clinical attacks without primaquine therapy against latent stages in liver. primaquine causes seriously threatening acute hemolytic anemia in patients having inherited glucose-6-phosphate dehydrogenase (g6pd) deficiency. access to safe primaquine therapy hinges upon the ability to confirm g6pd normal status. carestart g6pd, a qualitative g6pd rapid diagnostic test (g6pd rdt) intended for use at point-of-care in impoverishe ... | 2016 | 26894297 |
| plasmodium malariae and plasmodium ovale infections in the china-myanmar border area. | the greater mekong subregion is aiming to achieve regional malaria elimination by 2030. though a shift in malaria parasite species predominance by plasmodium vivax has been recently documented, the transmission of the two minor plasmodium species, plasmodium malariae and plasmodium ovale spp., is poorly characterized in the region. this study aims to determine the prevalence of these minor species in the china-myanmar border area and their genetic diversity. | 2016 | 27846879 |
| low prevalence of laboratory-confirmed malaria in clinically diagnosed adult women from the wakiso district of uganda. | the malaria burden in sub-saharan africa (ssa) has fallen substantially. nevertheless, malaria remains a serious health concern, and uganda ranks third in ssa in total malaria burden. epidemiological studies of adult malaria in uganda are scarce and little is known about rates of malaria in non-pregnant adult women. this pilot study assessed malaria prevalence among adult women from wakiso district, historically a highly malaria endemic region. | 2016 | 27842555 |
| persistent parasitism: the adaptive biology of malariae and ovale malaria. | plasmodium malariae causes malaria in humans throughout the tropics and subtropics. plasmodium ovale curtisi and plasmodium ovale wallikeri are sympatric sibling species common in sub-saharan africa and also found in oceania and asia. although rarely identified as the cause of malaria cases in endemic countries, pcr detection has confirmed all three parasite species to be more prevalent, and persistent, than previously thought. chronic, low-density, multispecies asymptomatic infection is a succe ... | 2016 | 27480365 |
| genome-scale comparison of expanded gene families in plasmodium ovale wallikeri and plasmodium ovale curtisi with plasmodium malariae and with other plasmodium species. | malaria in humans is caused by six species of plasmodium parasites, of which the nuclear genome sequences for the two plasmodium ovale spp., p. ovale curtisi and p. ovale wallikeri, and plasmodium malariae have not yet been analyzed. here we present an analysis of the nuclear genome sequences of these three parasites, and describe gene family expansions therein. plasmodium ovale curtisi and p. ovale wallikeri are genetically distinct but morphologically indistinguishable and have sympatric range ... | 2016 | 27392654 |
| mouse-based research on quiescent primate malaria parasites. | mice engrafted with primate tissue make two important plasmodial dormancy-related questions researchable. the first is concerned with whether latent merozoites in the lymphatic system can give rise to relapse-like, recurrent malaria in primates. the second is that genetic evidence of hypnozoite activation as the source of relapsing primate malaria can be looked for. | 2016 | 26961183 |
| [epidemiology of imported malaria and entomological study of breeding sites of potential risk areas in the province of khemisset (morocco)]. | no case of autochthonous malaria has been detected in morocco since 2004. this achievement is due to a national strategy to combat the disease by appropriate and well-organized disease detection and treatment, as well as control of the mosquito vector of the disease, the female anopheles mosquito. nonetheless, imported malaria cases have been increasing (75 in 2007), due to the rise in international travel and migration from countries where the disease is endemic. this work is divided into two p ... | 2016 | 25295572 |
| the host specificity of ape malaria parasites can be broken in confined environments. | recent studies have revealed a large diversity of plasmodium spp. among african great apes. some of these species are related to plasmodium falciparum, the most virulent agent of human malaria (subgenus laverania), and others to plasmodium ovale, plasmodium malariae and plasmodium vivax (subgenus plasmodium), three other human malaria agents. laverania parasites exhibit strict host specificity in their natural environment. plasmodium reichenowi, plasmodium billcollinsi, plasmodium billbrayi and ... | 2016 | 27486075 |
| the increasing importance of plasmodium ovale and plasmodium malariae in a malaria elimination setting: an observational study of imported cases in jiangsu province, china, 2011-2014. | following initiation of china's national malaria elimination action plan in 2010, indigenous malaria infections in jiangsu province decreased significantly. meanwhile imported plasmodium infections have increased substantially, particularly plasmodium ovale and plasmodium malariae. given the risk for malaria resurgence, there is an urgent need to understand the increase in imported p. ovale and p. malariae infections as china works to achieve national malaria elimination. | 2016 | 27604629 |
| characteristics of imported malaria and species of plasmodium involved in shandong province, china (2012-2014). | malaria remains a serious public health problem in shandong province, china; therefore, it is important to explore the characteristics of the current malaria prevalence situation in the province. in this study, data of malaria cases reported in shandong during 2012-2014 were analyzed, and plasmodium species were confirmed by smear microscopy and nested-pcr. a total of 374 malaria cases were reported, 80.8% of which were reported from 6 prefectures. of all cases, p. falciparum was dominant (81.3% ... | 2016 | 27658591 |
| malaria in children of tshimbulu (western kasai, democratic republic of the congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting. | the literature data on malaria in western kasai, drc, are limited and inadequate. a recent molecular survey there has detected plasmodium ovale and plasmodium malariae as mixed infections with plasmodium falciparum. in tshimbulu, western kasai, during a humanitarian initiative designed to provide children with free preventive screening and to reduce the local high malaria death rate, accurate species identification was performed, in order to collect unambiguous epidemiological data and to evalua ... | 2016 | 26864461 |
| dengue infection as a potential trigger of an imported plasmodium ovale malaria relapse or a long incubation period in a non-endemic malaria region. | to report that dengue fever (df) could have triggered plasmodium ovale wallikeri malaria. | 2016 | 26809125 |
| screening for glucose-6-phosphate dehydrogenase deficiency using three detection methods: a cross-sectional survey in southwestern uganda. | despite the potential benefit of primaquine in reducing plasmodium falciparum transmission and radical cure of plasmodium vivax and plasmodium ovale infections, concerns over risk of hemolytic toxicity in individuals with glucose-6-phosphate dehydrogenase deficiency (g6pdd) have hampered its deployment. a cross-sectional survey was conducted in 2014 to assess the g6pdd prevalence among 631 children between 6 and 59 months of age in southwestern uganda, an area where primaquine may be a promising ... | 2016 | 27672207 |
| molecular evidence of high rates of asymptomatic p. vivax infection and very low p. falciparum malaria in botswana. | botswana is one of eight sadc countries targeting malaria elimination by 2018. through spirited upscaling of control activities and passive surveillance, significant reductions in case incidence of plasmodium falciparum (0.96 - 0.01) was achieved between 2008 and 2012. as part of the elimination campaign, active detection of asymptomatic plasmodium species by a highly sensitive method was deemed necessary. this study was carried out to determine asymptomatic plasmodium species carriage by nested ... | 2016 | 27682611 |
| non-falciparum malaria in dakar: a confirmed case of plasmodium ovale wallikeri infection. | plasmodium ovale is rarely described in senegal. a case of clinical malaria due to p. ovale wallikeri in west central of senegal is reported. | 2016 | 27557982 |
| transfusion-transmitted severe plasmodium knowlesi malaria in a splenectomized patient with beta-thalassaemia major in sabah, malaysia: a case report. | transfusion-transmitted malaria (ttm) is a well-recognized risk of receiving blood transfusions, and has occurred with plasmodium falciparum, plasmodium vivax, plasmodium ovale, and plasmodium malariae. the simian parasite plasmodium knowlesi is also known to be transmissible through inoculation of infected blood, and this species is now the most common cause of malaria in malaysia with a high rate of severity and fatal cases reported. no confirmed case of accidental transfusion-transmitted p. k ... | 2016 | 27405869 |
| low prevalence of plasmodium malariae and plasmodium ovale mono-infections among children in the democratic republic of the congo: a population-based, cross-sectional study. | in an effort to improve surveillance for epidemiological and clinical outcomes, rapid diagnostic tests (rdts) have become increasingly widespread as cost-effective and field-ready methods of malaria diagnosis. however, there are concerns that using rdts specific to plasmodium falciparum may lead to missed detection of other malaria species such as plasmodium malariae and plasmodium ovale. | 2016 | 27392905 |
| climate, environment and transmission of malaria. | malaria, the most common parasitic disease in the world, is transmitted to the human host by mosquitoes of the genus anopheles. the transmission of malaria requires the interaction between the host, the vector and the parasite.the four species of parasites responsible for human malaria are plasmodium falciparum, plasmodium ovale, plasmodium malariae and plasmodium vivax. occasionally humans can be infected by several simian species, like plasmodium knowlesi, recognised as a major cause of human ... | 2016 | 27367318 |
| common asymptomatic and submicroscopic malaria infections in western thailand revealed in longitudinal molecular and serological studies: a challenge to malaria elimination. | despite largely successful control efforts, malaria remains a significant public health problem in thailand. based on microscopy, the northwestern province of tak, once thailand's highest burden area, is now considered a low-transmission region. however, microscopy is insensitive to detect low-level parasitaemia, causing gross underestimation of parasite prevalence in areas where most infections are subpatent. the objective of this study was to assess the current epidemiology of malaria prevalen ... | 2016 | 27333893 |
| seroepidemiology of plasmodium species infections in zimbabwean population. | individuals living in malaria-endemic regions may be exposed to more than one plasmodium species; there is paucity of data on the distribution of the different species of plasmodium in affected populations, in part due to the diagnostic method of microscopy, which cannot easily differentiate between the species. sero-epidemiological data can overcome some of the shortcomings of microscopy. | 2016 | 27165412 |
| investigation on possible transmission of monkeys' plasmodium to human in a populations living in the equatorial rainforest of the democratic republic of congo. | plasmodiums are protozoa that may infect various hosts. only five species are now recognized as naturally parasitizing humans: plasmodium falciparum, plasmodium vivax, plasmodium malariae, plasmodium ovale and plasmodium knowlesi. this fifth species, p. knowlesi, previously identified as naturally parasitizing the monkey macaca fascicularis, has been microscopically confused for a long time with p. malariae or p. falciparum and it was not possible to correctly differentiate them until the advent ... | 2016 | 27141437 |
| clinical implications of a gradual dormancy concept in malaria. | malaria recurrences after an initially successful therapy and malarial fever occurring a long time after infection are well-known problems in malariology. currently, two distinct types of malaria recurrences are defined: recrudescence and relapse. a recrudescence is thought to originate from circulating plasmodium blood stages which do not cause fever before a certain level of a microscopically detectable parasitemia is reached. contrary, a relapse is thought to originate from quiescent intracel ... | 2016 | 27079460 |
| the risk of imported malaria in security forces personnel returning from overseas missions in the context of prevention of re-introduction of malaria to sri lanka. | sri lanka is a malaria-free country. however it remains surrounded by countries with endemic malaria transmission. since the last indigenous case of malaria was reported in october 2012, only imported malaria cases have been diagnosed with 36 cases detected in 2015, which includes 17 cases each of plasmodium vivax and plasmodium falciparum and two cases of plasmodium ovale. | 2016 | 26955813 |
| human malaria diagnosis using a single-step direct-pcr based on the plasmodium cytochrome oxidase iii gene. | nested pcrs based on the plasmodium 18s-rrna gene have been extensively used for human malaria diagnosis. however, they are not practical when large quantities of samples need to be processed, further there have been challenges in the performance and when interpreting results, especially when submicroscopic infections are analysed. here the use of "direct pcr" was investigated with the aim of improving diagnosis in the malaria elimination era. | 2016 | 26928594 |
| uk malaria treatment guidelines 2016. | 1.malaria is the tropical disease most commonly imported into the uk, with 1300-1800 cases reported each year, and 2-11 deaths. 2. approximately three quarters of reported malaria cases in the uk are caused by plasmodium falciparum, which is capable of invading a high proportion of red blood cells and rapidly leading to severe or life-threatening multi-organ disease. 3. most non-falciparum malaria cases are caused by plasmodium vivax; a few cases are caused by the other species of plasmodium: pl ... | 2016 | 26880088 |
| non-falciparum malaria infections in pregnant women in west africa. | non-plasmodium falciparum malaria infections are found in many parts of sub-saharan africa but little is known about their importance in pregnancy. | 2016 | 26823277 |
| performance of microscopy for the diagnosis of malaria and human african trypanosomiasis by diagnostic laboratories in the democratic republic of the congo: results of a nation-wide external quality assessment. | the present external quality assessment (eqa) assessed microscopy of blood parasites among diagnostic laboratories in the democratic republic of the congo. the eqa addressed 445 participants in 10/11 provinces (october 2013-april 2014). participants were sent a panel of five slides and asked to return a routinely stained slide which was assessed for quality of preparation and staining. response rate was 89.9% (400/445). for slide 1 (no parasites), 30.6% participants reported malaria, mostly plas ... | 2016 | 26788725 |
| loop-mediated isothermal amplification assay for identification of five human plasmodium species in malaysia. | the lack of rapid, affordable, and accurate diagnostic tests represents the primary hurdle affecting malaria surveillance in resource- and expertise-limited areas. loop-mediated isothermal amplification (lamp) is a sensitive, rapid, and cheap diagnostic method. five species-specific lamp assays were developed based on 18s rrna gene. sensitivity and specificity of lamp results were calculated as compared with microscopic examination and nested polymerase chain reaction. lamp reactions were highly ... | 2016 | 26598573 |
| case report: spontaneous rupture of spleen in patient with plasmodium ovale malaria. | malaria may lead to spontaneous splenic rupture as a rare but potentially lethal complication. most frequently, this has been reported in patients infected with plasmodium falciparum and plasmodium vivax, while other parasitic agents are less likely to be the cause.we report a 29-year-old british caucasian, who after returning from a business trip in democratic republic congo was diagnosed with tertian malaria caused by plasmodium ovale.during his in-patient stay, the patient suffered a splenic ... | 2016 | 26542132 |
| malaria in three epidemiological strata in mauritania. | malaria epidemiology in mauritania has been characterized on the basis of epidemiological strata, defined by climatic and geographic features, which divide the country into three zones: sahelian zone, sahelo-saharan transition zone, and saharan zone. the association between geographic stratification and malaria transmission was assessed through a series of parasitological and entomological surveys. | 2016 | 27068219 |
| diagnosis of an imported plasmodium ovale wallikeri infection in malaysia. | plasmodium ovale is rare and not exactly known to be autochthonous in malaysia. there are two distinct forms of the parasite, namely p. ovale curtisi (classic form) and p. ovale wallikeri (variant form). here, the first sequence confirmed case of an imported p. ovale wallikeri infection in malaysia is presented. microscopy found plasmodium parasites with morphology similar to p. ovale or plasmodium vivax in the blood films. further confirmation using polymerase chain reaction (pcr) targeting the ... | 2016 | 26738724 |
| widespread distribution of plasmodium vivax malaria in mauritania on the interface of the maghreb and west africa. | plasmodium vivax is very rarely seen in west africa, although specific detection methods are not widely applied in the region, and it is now considered to be absent from north africa. however, this parasite species has recently been reported to account for most malaria cases in nouakchott, the capital of mauritania, which is a large country at the interface of sub-saharan west africa and the maghreb region in northwest africa. | 2016 | 26861780 |
| molecular characterization of misidentified plasmodium ovale imported cases in singapore. | plasmodium ovale, considered the rarest of the malaria parasites of humans, consists of two morphologically identical but genetically distinct sympatric species, plasmodium ovale curtisi and plasmodium ovale wallikeri. these parasites resemble morphologically to plasmodium vivax with which they also share a tertian periodicity and the ability to cause relapses, making them easily misidentified as p. vivax. plasmodium ovale infections are rarely reported, but given the likelihood of misidentifica ... | 2015 | 26577930 |
| plasmodium falciparum full life cycle and plasmodium ovale liver stages in humanized mice. | experimental studies of plasmodium parasites that infect humans are restricted by their host specificity. humanized mice offer a means to overcome this and further provide the opportunity to observe the parasites in vivo. here we improve on previous protocols to achieve efficient double engraftment of tk-nog mice by human primary hepatocytes and red blood cells. thus, we obtain the complete hepatic development of p. falciparum, the transition to the erythrocytic stages, their subsequent multipli ... | 2015 | 26205537 |
| malaria case in madagascar, probable implication of a new vector, anopheles coustani. | indoor spraying of insecticides and the use of insecticide-treated bed nets are key strategies for national malaria vector control in the central highlands of madagascar. during the year 2013, malaria outbreaks were reported by the national malaria control programme in the highlands, including the district of ankazobe. | 2015 | 26620552 |
| quality of malaria diagnosis and molecular confirmation of plasmodium ovale curtisi in a rural area of the southeastern region of ethiopia. | approximately 50 million people (60 %) live in malaria risk areas in ethiopia, at altitudes below 2000 m. according to official data, 60-70 % of malaria cases are due to plasmodium falciparum, and 40-30 % by plasmodium vivax. the species plasmodium ovale was detected in 2013 in the northwest of the country, being the first report of the presence of this species in ethiopia since the 60 s. the aim of this study was to assess the diagnosis by microscopy and pcr, and demonstrate the presence of oth ... | 2015 | 26383920 |
| a molecular survey of acute febrile illnesses reveals plasmodium vivax infections in kedougou, southeastern senegal. | control efforts towards malaria due to plasmodium falciparum significantly decreased the incidence of the disease in many endemic countries including senegal. surprisingly, in kedougou (southeastern senegal) p. falciparum malaria remains highly prevalent and the relative contribution of other plasmodium species to the global malaria burden is very poorly documented, partly due to the low sensitivity of routine diagnostic tools. molecular methods offer better estimate of circulating plasmodium sp ... | 2015 | 26186936 |
| detection of human malaria using recombinant plasmodium knowlesi merozoire surface protein-1 (msp-1₁₉) expressed in escherichia coli. | malaria remains one of the world's most important infectious diseases and is responsible for enormous mortality and morbidity. human infection with plasmodium knowlesi is widely distributed in southeast asia. merozoite surface protein-1₁₉ (msp-1₁₉), which plays an important role in protective immunity against asexual blood stage malaria parasites, appears as a leading immunogenic antigen of plasmodium sp. we evaluated the sensitivity and specificity of recombinant p. knowlesi msp-1₁₉ (rmsp-1₁₉) ... | 2015 | 25812552 |
| molecular investigation of mixed malaria infections in southwest saudi arabia. | to investigate the incidence of mixed-species (ms) malaria infection, and compare the results with microscopically confirmed cases of malaria. | 2015 | 25719595 |
| sympatric distribution of plasmodium ovale curtisi and p. ovale wallikeri in india: implication for the diagnosis of malaria and its control. | we report the first evidence of sympatric distribution of plasmodium ovale curtisi and p. ovale wallikeri from india. | 2015 | 25716936 |
| complicated malaria: a rare presentation of plasmodium ovale. | malaria has emerged as a major public health problem worldwide. complications are commonly seen in plasmodium falciparum (p. falciparum) and plasmodium vivax (p. vivax) infection, but due to plasmodium ovale (p. ovale) infection is rarely described in literature. here we report a case of severe disease due to p. ovale infection complicated with jaundice, thrombocytopenia, hypotension and acute renal failure. | 2015 | 25672340 |
| molecular detection of human plasmodium species in sabah using plasmonex™ multiplex pcr and hydrolysis probes real-time pcr. | malaria is a vector borne-parasitic disease transmitted through the bite of the infective female anopheles mosquitoes. five plasmodium species have been recognized by world health organization (who) as the causative agents of human malaria. generally, microscopic examination is the gold standard for routine malaria diagnosis. however, molecular pcr assays in many cases have shown improvement on the sensitivity and specificity over microscopic or other immunochromatographic assays. | 2015 | 25651852 |
| mass screening and treatment on the basis of results of a plasmodium falciparum-specific rapid diagnostic test did not reduce malaria incidence in zanzibar. | seasonal increases in malaria continue in hot spots in zanzibar. mass screening and treatment (msat) may help reduce the reservoir of infection; however, it is unclear whether rapid diagnostic tests (rdts) detect a sufficient proportion of low-density infections to influence subsequent transmission. | 2015 | 25429102 |
| detection of intracellular parasites by use of the cellavision dm96 analyzer during routine screening of peripheral blood smears. | conventional microscopy is the gold standard for malaria diagnosis. the cellavision dm96 is a digital hematology analyzer that utilizes neural networks to locate, digitize, and preclassify leukocytes and characterize red blood cell morphology. this study compared the detection rates of plasmodium and babesia species on peripheral blood smears utilizing the cellavision dm96 with the rates for a routine red blood cell morphology scan. a total of 281 slides were analyzed, consisting of 130 slides p ... | 2015 | 25378575 |
| ecology of malaria infections in western lowland gorillas inhabiting dzanga sangha protected areas, central african republic. | african great apes are susceptible to infections with several species of plasmodium, including the predecessor of plasmodium falciparum. little is known about the ecology of these pathogens in gorillas. a total of 131 gorilla fecal samples were collected from dzanga-sangha protected areas to study the diversity and prevalence of plasmodium species. the effects of sex and age as factors influencing levels of infection with plasmodium in habituated gorilla groups were assessed. ninety-five human b ... | 2015 | 25736484 |
| late relapse of imported plasmodium ovale malaria: a case report. | we report the first case of an imported plasmodium ovale relapse in a tunisian man who developed malaria three years after leaving sub- saharan africa. a 29-year-old tunisian man consulted in september 2011 because of a fever, myalgia, and headache that had begun eight days earlier and persisted despite treatment with oral antibiotics. on questioning, the patient stated that he had resided three years ago for six months in ivory coast, where he acquired malaria. he was treated with artemether-lu ... | 2015 | 26644094 |
| strategies for understanding and reducing the plasmodium vivax and plasmodium ovale hypnozoite reservoir in papua new guinean children: a randomised placebo-controlled trial and mathematical model. | the undetectable hypnozoite reservoir for relapsing plasmodium vivax and p. ovale malarias presents a major challenge for malaria control and elimination in endemic countries. this study aims to directly determine the contribution of relapses to the burden of p. vivax and p. ovale infection, illness, and transmission in papua new guinean children. | 2015 | 26505753 |
| [13-year old girl with fever 6 months after returning from sub-saharan africa]. | a 13-year-old girl presented with regular fevers, 6 months after a prolonged trip through africa. the patient reported relapsing fevers at 48 hour intervals. each febrile episode was followed by pronounced fatigue and a subsequent recovery back to her usual state of health. she reported having taken weekly mefloquine during and after the trip to africa. | 2015 | 26488102 |
| fever in the returning traveller: the importance of sensitivity. | we report the case of a 28-year-old man, presenting with episodes of fever and rigours, having recently returned from cameroon and uganda. initial investigations for malaria were negative, and the patient was sent home without a clear diagnosis. subsequent review of the blood film revealed the presence of plasmodium ovale. this case highlights the importance of repeated and careful inspection of blood films, given the relatively low sensitivity of rapid diagnostic tests in p. ovale infection. it ... | 2015 | 26464405 |
| lord byron's death: a case of late malarial relapse? | the study examines the pathological circumstances related to byron's death, the primary issue being malaria. lord byron died during the greek war of independence against the ottoman empire, in messolonghi on 19 april 1824. byron's medical profile consists of recurrent onsets of fever, which gave rise to the issue of malaria relapses. according to byron's letters he reported crises of fever in greece (1810), malta (1811), italy (1817-1819) and england. evidence from byron's autopsy, specifically ... | 2015 | 26397304 |
| [diagnosis of an imported case of plasmodium ovale infection in nanping city, fujian]. | an imported case previously misdiagnosed as vivax malaria was reviewed. the epidemiological data and blood sample were collected. the detection was conducted by microscopy, rapid diagnostic test (rdt) and nested pcr. the case was finally comfirmed as the first imported case of plasmodium ovale infection in nanping. | 2015 | 26080533 |
| [application of nested pcr in the diagnosis of imported plasmodium ovale infection]. | to identity plasmodium ovale infection by 18s rrna gene nested pcr. | 2015 | 26080528 |
| dimorphism in genes encoding sexual-stage proteins of plasmodium ovale curtisi and plasmodium ovale wallikeri. | plasmodium ovale curtisi and plasmodium ovale wallikeri are distinct species of malaria parasite which are sympatric throughout the tropics, except for the americas. despite this complete overlap in geographic range, these two species do not recombine. although morphologically very similar, the two taxa must possess distinct characters which prevent recombination between them. we hypothesised that proteins required for sexual reproduction have sufficiently diverged between the two species to pre ... | 2015 | 25817462 |
| do hypnozoites cause relapse in malaria? | the concept that hypnozoites give rise to relapses in plasmodium vivax and plasmodium ovale malaria has become dogma. however, it is evident from particular contemporary research findings that hypnozoites are not necessarily the origin of all relapse-like recurrences of malaria caused by these parasites. this is the core opinion presented, and i discuss it fully. the hypnozoite theory of relapse needs to be re-evaluated in view of the recent, increased focus on p. vivax and liver stages of plasm ... | 2015 | 25816801 |
| first imported plasmodium ovale malaria in central america: case report of a guatemalan soldier and a call to improve its accurate diagnosis. | the mesoamerican ministers of health have set 2020 as the target for malaria elimination to be achieved in the region. imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence. we report the first imported plasmodium ovale infection with molecular confirmation in central america, which occurred in a guatemalan soldier that had been deployed in africa. the obstacles for its diagnosis using the standard microscopy technique and the need to ... | 2015 | 25722882 |
| establishing a china malaria diagnosis reference laboratory network for malaria elimination. | in china, the prevalence of malaria has reduced dramatically due to the elimination programme. the continued success of the programme will depend upon the accurate diagnosis of the disease in the laboratory. the basic requirements for this are a reliable malaria diagnosis laboratory network and quality management system to support case verification and source tracking. | 2015 | 25628071 |
| characterization of plasmodium ovale curtisi and p. ovale wallikeri in western kenya utilizing a novel species-specific real-time pcr assay. | plasmodium ovale is comprised of two genetically distinct subspecies, p. ovale curtisi and p. ovale wallikeri. although p. ovale subspecies are similar based on morphology and geographical distribution, allelic differences indicate that p. ovale curtisi and p. ovale wallikeri are genetically divergent. additionally, potential clinical and latency duration differences between p. ovale curtisi and p. ovale wallikeri demonstrate the need for investigation into the contribution of this neglected mal ... | 2015 | 25590587 |
| optimal primaquine use for radical cure of plasmodium vivax and plasmodium ovale malaria in japanese travelers--a retrospective analysis. | recently, a dose of 30 mg (base) primaquine daily for 14 days is increasingly recommended for radical cure of plasmodium vivax malaria. however, total primaquine doses, or those per body weight, are also recognized as important. in japan, primaquine is not a licensed medicine, but has been used through the research group on chemotherapy of tropical diseases for >3 decades. | 2015 | 25482427 |
| performance of rapid diagnostic tests for plasmodium ovale malaria in japanese travellers. | rapid diagnostic tests (rdts) are used widely in the diagnosis of malaria. although the effectiveness of rdts for malaria has been described in many previous studies, the low performance of rdt particularly for plasmodium ovale malaria in traveller has rarely been reported. | 2014 | 25473374 |
| [diagnosis of the first imported case of plasmodium ovale infection at guangdong port]. | the first imported case of plasmodium ovale infection in guangdong province was identified. the patient worked in myanmar for one week and had a fever when he arrived at guangzhou baiyun international airport. epidemiological information and blood sample were collected. the detection was conducted by microscopy, right view rapid malaria test (rdts) and real-time pcr with plasmodium genus-specific and species-specific primers and probes. the case showed weak positive rdt result, and was confirmed ... | 2014 | 25065219 |
| new type of ssurdna sequence was detected from both plasmodium ovale curtisi and plasmodium ovale wallikeri samples. | plasmodium ovale is relatively unfamiliar to chinese staff engaged in malaria diagnosis. in 2013, dried blood spots of four unidentified but suspected ovale malaria samples were sent to the national malaria reference laboratory (nmrl) for reconfirmation. | 2014 | 24893846 |
| [overviews on plasmodium ovale wallikeri and its genetic detection system]. | in recent years, some microscopy-positive but nest-pcr negative (based on small subunit ribosomal rna gene) ovale malaria cases were reported. the detection failure was due to the genetic variations between plasomdoium ovale wallikeri and p. ovale curtisi. this paper reviews the discovery and validation processes of p. ovale wallikeri and the detection systems for identification of p. ovale. | 2014 | 24822370 |
| [iditification of five imported cases of plasmodium ovale wallikeri infection in zhejiang province]. | to identify and analyze plasmodium ovale wallikeri in 5 imported malaria cases, who were detected positive by microscopy and negative by conventional pcr. | 2014 | 25726600 |
| severe morbidity and mortality risk from malaria in the united states, 1985-2011. | recent reports of plasmodium vivax associated with severe syndromes and mortality from malaria endemic areas questions the "benign" course of non-falciparum malarias. | 2014 | 25734104 |
| a morphology-based method for the diagnosis of red blood cells parasitized by plasmodium malariae and plasmodium ovale. | the morphology of red blood cells (rbcs) is altered significantly during the maturation stages of malaria parasites, which include ring, trophozoite, and schizont. there is dissimilarity in terms of the morphological characteristics of parasitized rbcs infected by the 4 species of plasmodium, including falciparum, vivax, malariae, and ovale. this makes the process of diagnosis very difficult, which may lead to a wrong treatment method and substantial damage to the health of the patient. an innov ... | 2014 | 24568595 |
| a 20-year longitudinal study of plasmodium ovale and plasmodium malariae prevalence and morbidity in a west african population. | plasmodium ovale and plasmodium malariae have long been reported to be widely distributed in tropical africa and in other major malaria-endemic areas of the world. however, little is known about the burden caused by these two malaria species. | 2014 | 24520325 |
| sequence-based optimization of a quantitative real-time pcr assay for detection of plasmodium ovale and plasmodium malariae. | although microscopic examination of giemsa-stained blood smears remains the gold standard for the diagnosis of malaria, molecular detection using pcr is becoming increasingly popular. due to discrepant pcr and microscopy results, we aimed to optimize our detection assays for plasmodium malariae and plasmodium ovale by sequencing the 18s rrna region and developing a new primer and probe set for real-time quantitative pcr (qpcr). clinical specimens positive for p. malariae (n = 15) or p. ovale (n ... | 2014 | 24430459 |
| malaria-induced splenic infarction. | splenic infarction is a rare complication of malaria. we report two recent cases of splenic infarction after plasmodium vivax infection. no systematic review of malaria-induced splenic infarction was available, therefore we conducted a systematic review of the english, french, and spanish literature in pubmed and koreamed for reports of malaria-associated splenic infarction from 1960 to 2012. of the 40 cases collected on splenic infarction by plasmodium species, 23 involved p. vivax, 11 plasmodi ... | 2014 | 25294615 |
| immunochromatographic antigen testing alone is sufficient to identify asymptomatic refugees at risk of severe malaria presenting to a single health service in victoria. | current screening guidelines for malaria in new refugees include a combination of thick and thin film examination and immunochromatographic antigen test (ict). however, as the prevalence of malaria in our population has decreased due to changing refugee demographics, we sought to determine if an ict alone can reliably exclude malaria in our asymptomatic refugee population.a retrospective analysis was conducted of all investigations for malaria performed from 1 august 2011 to 31 july 2013, includ ... | 2014 | 25158813 |
| malaria: an update on current chemotherapy. | chemotherapy of malaria has become a rapidly changing field. less than two decades ago, treatment regimens were increasingly bound to fail due to emerging drug resistance against 4-aminoquinolines and sulfa compounds. by now, artemisinin-based combination therapies (acts) constitute the standard of care for uncomplicated falciparum malaria and are increasingly also taken into consideration for the treatment of non-falciparum malaria. | 2014 | 25110058 |
| a molecular survey of febrile cases in malaria-endemic areas along china-myanmar border in yunnan province, people's republic of china. | imported malaria is a major threat to neighboring malaria-eliminating countries such as p.r. china and is difficult to monitor. a molecular survey of febrile patients with a history of traveling abroad along the myanmar-china endemic border areas from january 2008 to august 2012 was carried out. the rates of infection with species of plasmodium and compliance of microscopy diagnosis with nested pcr (polymerase chain reaction) results were calculated. | 2014 | 24954235 |
| the ears of the african elephant: unexpected high seroprevalence of plasmodium ovale and plasmodium malariae in healthy populations in western africa. | malaria is a life-threatening pathology in africa. plasmodium falciparum and plasmodium vivax attract the most focus because of their high prevalence and mortality. knowledge about the prevalence of the cryptic pathogens plasmodium ovale and plasmodium malariae is limited. thanks to recombinant tools, their seroprevalence was measured for the first time, as well as the prevalence of mixed infections in a malaria-asymptomatic population in benin, a malaria-endemic country. | 2014 | 24946685 |
| toward malaria eradication in saudi arabia: evidence from 4-year surveillance in makkah. | saudi arabia has implemented strategies for the eradication of malaria. however, influx of people from countries endemic for malaria for either employment or hajj makes the country highly susceptible to malaria importation. the makkah region is known to host millions of immigrants yearly and has a surveillance system to monitor the incidence of malaria. the objective of this study was to examine malaria patients, nationality, and parasite type in makkah region between 2008 and 2011. | 2014 | 24894785 |
| comparison of giemsa microscopy with nested pcr for the diagnosis of malaria in north gondar, north-west ethiopia. | malaria remains one of the leading communicable diseases in ethiopia. early diagnosis combined with prompt treatment is one of the main strategies for malaria prevention and control. despite its limitation, giemsa microscopy is still considered to be the gold standard for malaria diagnosis. this study aimed to compare the performance of giemsa microscopy with nested polymerase chain reaction (npcr) for the diagnosis of malaria in north-west ethiopia. | 2014 | 24884606 |
| detection of persistent plasmodium spp. infections in ugandan children after artemether-lumefantrine treatment. | during a longitudinal study investigating the dynamics of malaria in ugandan lakeshore communities, a consistently high malaria prevalence was observed in young children despite regular treatment. to explore the short-term performance of artemether-lumefantrine (al), a pilot investigation into parasite carriage after treatment(s) was conducted in bukoba village. a total of 163 children (aged 2-7 years) with a positive blood film and rapid antigen test were treated with al; only 8.7% of these had ... | 2014 | 24837880 |