Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| development of molecular markers tightly linked to pvr4 gene in pepper using next-generation sequencing. | it is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. potyvirus resistance 4 (pvr4) locus in pepper confers resistance to three pathotypes of potato virus y and to pepper mottle virus. we describe the use of next-generation sequencing technology to generate molecular markers tightly linked to pvr4. initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate p ... | 2017 | 25798050 |
| tobacco vein banding mosaic virus 6k2 protein hijacks nbpsbo1 for virus replication. | chloroplast-bound vesicles are key components in viral replication complexes (vrcs) of potyviruses. the potyviral vrcs are induced by the second 6 kda protein (6k2) and contain at least viral rna and nuclear inclusion protein b. to date, no chloroplast protein has been identified to interact with 6k2 and involve in potyvirus replication. in this paper, we showed that the photosystem ii oxygen evolution complex protein of nicotiana benthamiana (nbpsbo1) was a chloroplast protein interacting with ... | 2017 | 28230184 |
| potato virus y hcpro suppression of antiviral silencing in nicotiana benthamiana plants correlates with its ability to bind in vivo to 21- and 22-nucleotide small rnas of viral sequence. | we have investigated short and small rnas (srnas) that were bound to a biologically active hexahistidine-tagged potato virus y (pvy) hcpro suppressor of silencing, expressed from a heterologous virus vector in nicotiana benthamiana plants, and purified under non-denaturing conditions. we found that rnas in purified preparations were differentially enriched in srnas of 21 and to a much lesser extent of 22 nucleotides (nt) in length and of viral sequence (vsrnas) when compared to those found in a ... | 2017 | 28381573 |
| coilin, the signature protein of cajal bodies, differentially modulates the interactions of plants with viruses in widely different taxa. | cajal bodies (cbs) are distinct nuclear bodies physically and functionally associated with the nucleolus. in addition to their traditional function in coordinating maturation of certain nuclear rnas, cbs participate in cell cycle regulation, development, and regulation of stress responses. a key "signature" component of cbs is coilin, the scaffolding protein essential for cb formation and function. using an rna silencing (loss-of-function) approach, we describe here new phenomena whereby coilin ... | 2017 | 24637832 |
| potato virus y transmission efficiency from potato infected with single or multiple virus strains. | there has been a recent shift in the prevalence of potato virus y (pvy) strains affecting potato with the ordinary strain pvy(o) declining and the recombinant strains pvy(ntn) and pvy(n:o) emerging in the united states. multiple pvy strains are commonly found in potato fields and even in individual plants. factors contributing to the emergence of the recombinant strains are not well defined but differential aphid transmission of strains from single and mixed infections may play a role. we found ... | 2017 | 27938241 |
| virus surveys of capsicum spp. in the republic of benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species. | surveys were conducted in 2014 and 2015 in southern and northern benin, respectively, to identify the viruses infecting peppers (capsicum spp.). the samples were screened by elisa for cucumber mosaic virus (cmv), pepper veinal mottle virus (pvmv), potato virus y (pvy) and tomato yellow leaf curl virus (tylcv). a generic reverse transcription pcr (rt-pcr) was used to test for the presence of poleroviruses. elisa tests confirmed the prevalence of all viruses, while the rt-pcr detected pepper vein ... | 2017 | 28220324 |
| graphical genotyping as a method to map ny (o,n)sto and gpa5 using a reference panel of tetraploid potato cultivars. | the method of graphical genotyping is applied to a panel of tetraploid potato cultivars to visualize haplotype sharing. the method allowed to map genes involved in virus and nematode resistance. the physical coordinates of the amount of linkage drag surrounding these genes are easily interpretable. graphical genotyping is a visually attractive and easily interpretable method to represent genetic marker data. in this paper, the method is extended from diploids to a panel of tetraploid potato cult ... | 2017 | 27872942 |
| erratum: the effects of high temperature on infection by potato virus y, potato virus a, and potato leafroll virus. | [this corrects the article on p. 321 in vol. 32, pmid: 27493607.]. | 2017 | 28167894 |
| rnai-mediated simultaneous resistance against three rna viruses in potato. | rna interference (rnai) technology has been successfully applied in stacking resistance against viruses in numerous crop plants. during rnai, the production of small interfering rnas (sirnas) from template double-standard rna (dsrna) derived from expression constructs provides an on-switch for triggering homology-based targeting of cognate viral transcripts, hence generating a pre-programmed immunity in transgenic plants prior to virus infection. in the current study, transgenic potato lines (so ... | 2017 | 28194691 |
| adaptive evolution and demographic history contribute to the divergent population genetic structure of potato virus y between china and japan. | potato virus y (pvy) is an important plant pathogen causing considerable economic loss to potato production. knowledge of the population genetic structure and evolutionary biology of the pathogen, particularly at a transnational scale, is limited but vital in developing sustainable management schemes. in this study, the population genetic structure and molecular evolution of pvy were studied using 127 first protein (p1) and 137 coat protein (cp) sequences generated from isolates collected from p ... | 2017 | 28352297 |
| erratum to: bimodal dynamics of primary metabolism-related responses in tolerant potato-potato virus y interaction. | 2017 | 28288559 | |
| complete genome sequence of sunflower ring blotch virus, a new potyvirus infecting sunflower in argentina. | the complete genome sequence of sunflower ring blotch virus (surbv), a previously undescribed potyvirus infecting sunflower in argentina, is reported. the surbv genome comprises 9555 nucleotides (nt) and encodes a polyprotein of 3061 amino acids, flanked by 5' and 3' untranslated regions of 117 and 255 nt, respectively. phylogenetic analysis showed that surbv belongs to the potato virus y (pvy) subgroup and clusters together with sunflower chlorotic mottle virus and bidens mosaic virus. percenta ... | 2017 | 28224251 |
| a viral protease relocalizes in the presence of the vector to promote vector performance. | vector-borne pathogens influence host characteristics relevant to host-vector contact, increasing pathogen transmission and survival. previously, we demonstrated that infection with turnip mosaic virus, a member of one of the largest families of plant-infecting viruses, increases vector attraction and reproduction on infected hosts. these changes were due to a single viral protein, nia-pro. here we show that nia-pro responds to the presence of the aphid vector during infection by relocalizing to ... | 2017 | 28205516 |
| detection and molecular characterization of slovak tomato isolates belonging to two recombinant strains of potato virus y. | total rnas from a symptomless tomato plant were subjected to next-generation sequencing (ngs) analysis, revealing the presence of a single viral agent - potato virus y (pvy). the analysis of determined full-length genome sequence assigned the pvy sl16 isolate to the recombinant pvy-n-wi strain group. a series of primers targeting the four main recombinant junction (rj) sites were used for characterization of additional 5 tomato pvy isolates recovered in western slovakia. based on the partial seq ... | 2017 | 27928913 |
| dynamic expression analysis of early response genes induced by potato virus y in pvy-resistant nicotiana tabacum. | dynamic transcriptional changes of the host early responses genes were detected in pvy-resistant tobacco varieties infected with potato virus y; pvy resistance is a complex process that needs series of stress responses. potato virus y (pvy) causes a severe viral disease in cultivated crops, especially in solanum plants. to understand the molecular basis of plant responses to the pvy stress, suppression subtractive hybridization (ssh) and microarray approaches were combined to identify the potent ... | 2017 | 27896424 |
| a model to explain temperature dependent systemic infection of potato plants by potato virus y. | the effect of temperature on the rate of systemic infection of potatoes (solanum tuberosum l. cv. chu-baek) by potato virus y (pvy) was studied in growth chambers. systemic infection of pvy was observed only within the temperature range of 16°c to 32°c. within this temperature range, the time required for a plant to become infected systemically decreased from 14 days at 20°c to 5.7 days at 28°c. the estimated lower thermal threshold was 15.6°c and the thermal constant was 65.6 degree days. a sys ... | 2017 | 28381967 |
| quantitative trait loci in pepper control the effective population size of two rna viruses at inoculation. | infection of plants by viruses is a complex process involving several steps: inoculation into plant cells, replication in inoculated cells and plant colonization. the success of the different steps depends, in part, on the viral effective population size (ne), defined as the number of individuals passing their genes to the next generation. during infection, the virus population will undergo bottlenecks, leading to drastic reductions in ne and, potentially, to the loss of the fittest variants. th ... | 2017 | 28691663 |
| validation of reference genes for quantifying changes in gene expression in virus-infected tobacco. | to facilitate quantification of gene expression changes in virus-infected tobacco plants, eight housekeeping genes were evaluated for their stability of expression during infection by one of three systemically-infecting viruses (cucumber mosaic virus, potato virus x, potato virus y) or a hypersensitive-response-inducing virus (tobacco mosaic virus; tmv) limited to the inoculated leaf. five reference-gene validation programs were used to establish the order of the most stable genes for the system ... | 2017 | 28689086 |
| sequential acquisition of potato virus y strains by myzus persicae favors the transmission of the emerging recombinant strains. | in the past decade recombinant strains of potato virus y (pvy) have overtaken the ordinary strain, pvy(o), as the predominant viruses affecting the us seed potato crop. aphids may be a contributing factor in the emergence of the recombinant strains, but studies indicate that differences in transmission efficiency of individual pvy strains either from single or mixed infections, although variable, are not generally significant. multiple strains of pvy are present in all potato production areas an ... | 2017 | 28666897 |
| potato virus y (pvy) isolates from physalis peruviana are unable to systemically infect potato or pepper and form a distinct new lineage within the pvy(c) strain group. | poha, or cape gooseberry (physalis peruviana l.), is a plant species cultivated in hawaii for fresh fruit production. in 2015, an outbreak of virus symptoms occurred on poha farms in the south kohala district of the island of hawaii. the plants displayed mosaic, stunting, and leaf deformation, and produced poor fruit. initial testing found the problem associated with potato virus y (pvy) infection. six individual pvy isolates, named poha1 to poha6, were collected from field-grown poha plants and ... | 2017 | 28653578 |
| comparative genetic diversity of potato virus y populations based on coat protein gene. | potato virus y (pvy) is an important plant pathogen with a wide host range including economically important crops of potato, tobacco, tomato, and pepper. the coat protein gene has been commonly used in studying molecular biology of plant viruses including pvy. in this study, we used a large dataset of cp sequences from isolates collected across the world to assess the detailed molecular evolution of pvy populations with a focus on the iranian pvy population. phylogenetic analysis showed that the ... | 2017 | 28523922 |
| effects of aphidius gifuensis on the feeding behavior and potato virus y transmission ability of myzus persicae. | the stylet penetration behavior of aphids when feeding on plants is associated with virus acquisition and inoculation. aphidius gifuensis (ashmead) is a primary endoparasitoid of myzus persicae (sulzer) which is the most efficient vector of plant viruses. information about the effects of parasitoid on aphid and virus transmission can provide an essential foundation for designing effective biological control strategies. this study aimed to investigate the effects of a. gifuensis on the feeding be ... | 2017 | 28485823 |
| phylogenetic study of recombinant strains of potato virus y. | potato virus y (pvy) exists as a complex of strains, including a growing number of recombinants. evolution of pvy proceeds through accumulation of mutations and more rapidly through recombination. here, the role of recombination in pvy evolution and the origin of common pvy recombinants were studied through whole genome analysis of 119 newly sequenced pvy isolates largely from u.s. potato, and subsequent combined phylogenetic and recombination analyses with an additional 166 whole pvy genomes fr ... | 2017 | 28399436 |
| vector population growth and condition-dependent movement drive the spread of plant pathogens. | plant viruses, often spread by arthropod vectors, impact natural and agricultural ecosystems worldwide. intuitively, the movement behavior and life history of vectors influence pathogen spread, but the relative contribution of each factor has not been examined. recent research has highlighted the influence of host infection status on vector behavior and life history. here, we developed a model to explore how vector traits influence the spread of vector-borne plant viruses. we allowed vector life ... | 2017 | 28555726 |
| facile synthesis of novel vanillin derivatives incorporating a bis(2-hydroxyethyl)dithhioacetal moiety as antiviral agents. | a series of vanillin derivatives incorporating a bis(2-hydroxyethyl)dithioacetal moiety was designed and synthesized via a facile method. a plausible reaction pathway was proposed and verified by computational studies. bioassay results demonstrated that target compounds possessed good to excellent activities against potato virus y (pvy) and cucumber mosaic virus (cmv), of which, compound 6f incorporating a bis(2-hydroxyethyl)dithioacetal moiety, exhibited the best curative and protection activit ... | 2017 | 28545296 |
| identification of mapks as signal transduction components required for the cell death response during compatible infection by the synergistic pair potato virus x-potato virus y. | systemic necrosis is one of the most severe symptoms caused in compatible plant-virus interactions and shares common features with the hypersensitive response (hr). mitogen-activated protein kinase (mapk) cascades are associated with responses to compatible and incompatible host-virus interactions. here, we show that virus-induced gene silencing of the nicotiana benthamiana mapk genes salicylic acid-induced protein kinase (sipk) and wound-induced protein kinase (wipk), and the mapk kinase (mapkk ... | 2017 | 28647505 |
| a novel recombined potato virus y isolate in china. | this study reports the findings of a distinct potato virus y (pvy) isolate found in northeast china. one hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of harbin in heilongjiang province of china. the collected tubers were planted and let to grow in a greenhouse. new potato plants generated from these tubers showed similar symptoms, except for one plant. subsequent serological analyses revealed pvy as the causing agent of the ... | 2017 | 28811755 |
| breeding of a new potato variety 'nagasaki kogane' with high eating quality, high carotenoid content, and resistance to diseases and pests. | 'nagasaki kogane' is a new potato variety bred from a cross between 'saikai 35' as a female parent and 'saikai 33' as a male. 'saikai 35' is resistant to bacterial wilt, contains the h1 and rychc genes for resistance to the potato cyst nematode (pcn) and potato virus y (pvy), respectively, and has high carotenoid content, while 'saikai 33' has large and high-yielding tubers and is resistant to both bacterial wilt and pcn. the carotenoid content of 'nagasaki kogane' is higher than that of 'dejima ... | 2017 | 28744186 |
| silencing the tobacco gene for rna-dependent rna polymerase 1 and infection by potato virus y cause remodeling of cellular organelles. | rna-dependent rna polymerase 1 (rdr1) has been shown to be involved in dna methylation, rna silencing and regulating expression of other genes. rdr1 gene expression is stimulated by infection with potato virus y° (pvy). transgenic nicotiana tabacum plants silenced for rdr1 gene expression showed morphological changes in mesophyll cells, associated with remodeling of the nuclei, chloroplasts and mitochondria. rdr1 silencing led to decreased nuclear size, increased heterochromatin content and aggr ... | 2017 | 28719835 |
| the effects of potato virus y-derived virus small interfering rnas of three biologically distinct strains on potato (solanum tuberosum) transcriptome. | potato virus y (pvy) is one of the most economically important pathogen of potato that is present as biologically distinct strains. the virus-derived small interfering rnas (vsirnas) from potato cv. russet burbank individually infected with pvy-n, pvy-ntn and pvy-o strains were recently characterized. plant defense rna-silencing mechanisms deployed against viruses produce vsirnas to degrade homologous viral transcripts. based on sequence complementarity, the vsirnas can potentially degrade host ... | 2017 | 28716126 |
| time-sampled population sequencing reveals the interplay of selection and genetic drift in experimental evolution of potato virus y. | rna viruses are one of the fastest-evolving biological entities. within their hosts, they exist as genetically diverse populations (i.e., viral mutant swarms), which are sculpted by different evolutionary mechanisms, such as mutation, natural selection, and genetic drift, and also the interactions between genetic variants within the mutant swarms. to elucidate the mechanisms that modulate the population diversity of an important plant-pathogenic virus, we performed evolution experiments with pot ... | 2017 | 28592544 |
| the phylogenetics of the global population of potato virus y and its necrogenic recombinants. | potato virus y (pvy) is a major pathogen of potatoes and other solanaceous crops worldwide. it is most closely related to potyviruses first or only found in the americas, and it almost certainly originated in the andes, where its hosts were domesticated. we have inferred the phylogeny of the published genomic sequences of 240 pvy isolates collected since 1938 worldwide, but not the andes. all fall into five groupings, which mostly, but not exclusively, correspond with groupings already devised u ... | 2017 | 28458913 |
| a recombinant potato virus y infectious clone tagged with the rosea1 visual marker (pvy-ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants. | potato virus y (pvy) is a major threat to the cultivation of potato and other solanaceous plants. by inserting a cdna coding for the antirrhinum majus rosea1 transcription factor into a pvy infectious clone, we created a biotechnological tool (pvy-ros1) that allows infection by this relevant plant virus to be tracked by the naked eye with no need for complex instrumentation. rosea1 is an myb-type transcription factor whose expression activates the biosynthesis of anthocyanin pigments in a dose-s ... | 2017 | 28428782 |
| integrated analysis of tobacco mirna and mrna expression profiles under pvy infection provids insight into tobacco-pvy interactions. | potato virus y (pvy) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.to explore the molecular pvy-host interactions, we analysed changes in the mirna and mrna profiles of tobacco in response to pvy infection. a total of 81 differentially expressed mirnas belonging to 29 families and 8133 mrnas were identified. the gene ontology (go) enrichment analyses showed that genes encoding the ... | 2017 | 28687775 |
| comparison between proteome and transcriptome response in potato (solanum tuberosum l.) leaves following potato virus y (pvy) infection. | plant diseases caused by viral infection are affecting all major crops. being an obligate intracellular organisms, chemical control of these pathogens is so far not applied in the field except to control the insect vectors of the viruses. understanding of molecular responses of plant immunity is therefore economically important, guiding the enforcement of crop resistance. to disentangle complex regulatory mechanisms of the plant immune responses, understanding system as a whole is a must. howeve ... | 2017 | 28684682 |
| dynamics of pvy strains in field grown potato: impact of strain competition and ability to overcome host resistance mechanisms. | potato virus y (pvy) is the most important viral pathogen affecting potato crops worldwide. pvy can be transmitted non-persistently by aphids that do not colonize the host plant, resulting in a rapid acquisition and transmission of the virus between plants. pvy exists as a complex of strains that can be distinguished according to their pathogenicity, serology and genomic analysis. while virus incidence remains low in scottish seed potato crops, pvy has become the increasingly prevalent virus. th ... | 2017 | 28625667 |
| primary metabolism, phenylpropanoids and antioxidant pathways are regulated in potato as a response to potato virus y infection. | potato production is one of the most important agricultural sectors, and it is challenged by various detrimental factors, including virus infections. to control losses in potato production, knowledge about the virus-plant interactions is crucial. here, we investigated the molecular processes in potato plants as a result of potato virus y (pvy) infection, the most economically important potato viral pathogen. we performed an integrative study that links changes in the metabolome and gene expressi ... | 2016 | 26727123 |
| a tilling approach to generate broad-spectrum resistance to potyviruses in tomato is hampered by eif4e gene redundancy. | genetic resistance to pathogens is important for sustainable maintenance of crop yields. recent biotechnologies offer alternative approaches to generate resistant plants by compensating for the lack of natural resistance. tomato (solanum lycopersicum) and related species offer a model in which natural and tilling-induced potyvirus resistance alleles may be compared. for resistance based on translation initiation factor eif4e1, we confirm that the natural allele sh-eif4e1(pi24)-pot1, isolated fro ... | 2016 | 26850324 |
| diversity of genetic backgrounds modulating the durability of a major resistance gene. analysis of a core collection of pepper landraces resistant to potato virus y. | the evolution of resistance-breaking capacity in pathogen populations has been shown to depend on the plant genetic background surrounding the resistance genes. we evaluated a core collection of pepper (capsicum annuum) landraces, representing the worldwide genetic diversity, for its ability to modulate the breakdown frequency by potato virus y of major resistance alleles at the pvr2 locus encoding the eukaryotic initiation factor 4e (eif4e). depending on the pepper landrace, the breakdown frequ ... | 2016 | 25967744 |
| prediction of host-derived mirnas with the potential to target pvy in potato plants. | potato virus y has emerged as a threatening problem in all potato growing areas around the globe. pvy reduces the yield and quality of potato cultivars. during the last 30 years, significant genetic changes in pvy strains have been observed with an increased incidence associated with crop damage. in the current study, computational approaches were applied to predict potato derived mirna targets in the pvy genome. the pvy genome is approximately 9 thousand nucleotides, which transcribes the follo ... | 2016 | 27683585 |
| a new eif4e1 allele characterized by rnaseq data mining is associated with resistance to potato virus y in tomato albeit with a low durability. | allele mining on susceptibility factors offers opportunities to find new sources of resistance among crop wild relatives for breeding purposes. as a proof of concept, we used available rnaseq data to investigate polymorphisms among the four tomato genes encoding translation initiation factors [eif4e1 and eif4e2, eifiso4e and the related gene new cap-binding protein(ncbp)] to look for new potential resistance alleles to potyviruses. by analysing polymorphism among rnaseq data obtained for 20 toma ... | 2016 | 27655175 |
| biological and molecular characterization of a tomato isolate of potato virus y (pvy) of the pvy(c) lineage. | an isolate of potato virus y (pvy), pvy-h14, was collected on the island of oahu, hawaii, from tomato plants exhibiting stunting and necrotic lesions on leaves. pvy-h14 triggered the hypersensitive resistance response in potato cultivars king edward and maris bard, typical of a pvy(c) strain, and was unable to infect systemically the four tested cultivars, desiree, maris bard, king edward, and russet norkotah. phylogenetic analysis of h14 and the whole genomes of 31 pvy isolates of non-recombina ... | 2016 | 27654668 |
| development and validation of high-resolution melting markers derived from rysto sts markers for high-throughput marker-assisted selection of potato carrying rysto. | sequence analysis of the chromosome region harboring the sequence-tagged site (sts) markers yes3-3a and yes3-3b for rysto, a gene responsible for extreme resistance to potato virus y (pvy) in potato, was performed in tetraploid potato 'barbara' (rrrr) and 'ac chaleur' (rrrr) as well as their progeny selections. three and two sequence variants were identified in barbara resistant (r) selections and ac chaleur susceptible (s) selections, respectively. further analysis indicates that the variant wi ... | 2016 | 27442536 |
| solanum venturii, a suitable model system for virus-induced gene silencing studies in potato reveals stmkk6 as an important player in plant immunity. | virus-induced gene silencing (vigs) is an optimal tool for functional analysis of genes in plants, as the viral vector spreads throughout the plant and causes reduced expression of selected gene over the whole plant. potato (solanum tuberosum) is one of the most important food crops, therefore studies performing functional analysis of its genes are very important. however, the majority of potato cultivars used in laboratory experimental setups are not well amenable to available vigs systems, thu ... | 2016 | 27213007 |
| autophagy induction in tobacco leaves infected by potato virus y(o) and its putative roles. | autophagy plays a critical role in the innate immune response of plants to pathogen infection. in the present study, we examined autophagy induced by potato virus y ordinary strain (pvy(o)) infection in tobacco (nicotiana benthamiana). enzyme-linked immunosorbent assays revealed that the number of virus particles in the plant peaked at 2 weeks post-inoculation and then gradually decreased. additionally, the amount of virus increased significantly in the 3rd and 4th leaves distal to the inoculate ... | 2016 | 27137843 |
| population analysis of iranian potato virus y isolates using complete genome sequence. | in this study, the full-length nucleotide sequences of four iranian pvy isolates belonging to pvy(n) strain were determined. the genome of iranian pvy isolates were 9,703-9,707 nucleotides long encoding all potyviral cistrons including p1, hc-pro, p3, 6k1, ci, 6k2, vpg, nia-pro, nib and cp with coding regions of 825, 1,395, 1,095, 156, 1,902, 156, 564, 732, 1,557 and 801 nucleotides in length, respectively. the length of pipo, embedded in the p3 cistron, was 231 nucleotides. phylogenetic analysi ... | 2016 | 26889113 |
| ecogenomic survey of plant viruses infecting tobacco by next generation sequencing. | the invasion of plant by viruses cause major damage to plants and reduces crop yield and integrity. devastating plant virus infection has been experienced at different times all over the world, which are attributed to different events of mutation, re-assortment and recombination occurring in the viruses. strategies for proper virus management has been mostly limited to eradicating the vectors that spreads the plant viruses. however, development of prompt and effective diagnostic methods are requ ... | 2016 | 27814723 |
| preferential acquisition and inoculation of pvyntn over pvyo in potato by the green peach aphid myzus persicae (sulzer). | in the past decade, the incidence and distribution of the recombinant, tuber necrotic strain of potato virus y (pvyntn) has been increasing in the us seed potato crop while the ordinary strain (pvyo) has been decreasing. the transmission efficiency of both strains was determined from two potato cultivars when acquired sequentially by the same aphid or when acquired by separate aphids and inoculated to the same plant. pvyntn was transmitted more efficiently than pvyo and the order of acquisition ... | 2016 | 26675892 |
| genetic determinism and evolutionary reconstruction of a host jump in a plant virus. | in spite of their widespread occurrence, only few host jumps by plant viruses have been evidenced and the molecular bases of even fewer have been determined. a combination of three independent approaches, 1) experimental evolution followed by reverse genetics analysis, 2) positive selection analysis, and 3) locus-by-locus analysis of molecular variance (amova) allowed reconstructing the potato virus y (pvy; genus potyvirus, family potyviridae) jump to pepper (capsicum annuum), probably from othe ... | 2016 | 26503941 |
| a new virus discovered by immunocapture of double-stranded rna, a rapid method for virus enrichment in metagenomic studies. | next-generation sequencing technologies enable the rapid identification of viral infection of diseased organisms. however, despite a consistent decrease in sequencing costs, it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. as the majority of plant viruses have an rna genome, a common approach is to extract the double-stranded rna (dsrna) replicative form, to enrich the replicating virus genetic material over the host background. the tradi ... | 2016 | 26990372 |
| multiarray on a test strip (mats): rapid multiplex immunodetection of priority potato pathogens. | multiarray on a test strip (mats) was developed for the detection of eight important potato pathogens. the proposed assay combines the rapidity of immunochromatography with the high throughput of array techniques. the test zone of the immunochromatographic strip comprises ordered rows of spots containing antibodies specific for different potato pathogens. the assay benefits from the simplicity of immunochromatography; colored immune complexes form at the corresponding spots within the test zone. ... | 2016 | 27007732 |
| biological, serological and molecular typing of potato virus y (pvy) isolates from tunisia. | in tunisia, potato virus y (pvy) currently presents a significant threat to potato production, reducing tuber yield and quality. three hundred and eighty-five potato samples (six different cultivars) collected in autumn 2007 from nine regions in tunisia were tested for pvy infection by das-elisa. the virus was detected in all regions surveyed, with an average incidence of 80.26%. subsequently, a panel of 82 tunisian pvy isolates (pvy-tn) was subjected to systematic biological, serological and mo ... | 2016 | 27813549 |
| contribution of noncolonizing aphids to potato virus y prevalence in potato in idaho. | potato virus y (pvy) is a major concern for potato production in the united states given its impact on both crop quality and yield. although green peach aphid, myzus persicae (sulzer), is the most efficient pvy vector, it may be less abundant in potato-growing areas of idaho relative to non-potato-colonizing aphid vectors of pvy that may disperse from nearby cereal fields and other crops. a field study was conducted during 2012-2013 to examine if noncolonizing aphids disperse to nearby potato fi ... | 2016 | 27694432 |
| combining abilities for agronomic traits and marker-assisted selection for potato virus x and potato virus y resistance. | disease-resistant potato cultivars with good tuber appearance and desirable agronomic traits are essential for meeting the demands of producers and the market. attaining these cultivars is the focus of potato breeding programs whose aim is to benefit the productive chain. the purpose of this study was to estimate combining abilities and evaluate potato clones based on tuber appearance, yield, and resistance to the pvy and pvx viruses. crosses between four commercial cultivars of potato with good ... | 2016 | 27706729 |
| contribution of noncolonizing aphids to potato virus y prevalence in potato in idaho. | potato virus y (pvy) is a major concern for potato production in the united states given its impact on both crop quality and yield. although green peach aphid, myzus persicae (sulzer), is the most efficient pvy vector, it may be less abundant in potato-growing areas of idaho relative to non-potato-colonizing aphid vectors of pvy that may disperse from nearby cereal fields and other crops. a field study was conducted during 2012-2013 to examine if noncolonizing aphids disperse to nearby potato fi ... | 2016 | 28028092 |
| the ire1/bzip60 pathway and bax inhibitor 1 suppress systemic accumulation of potyviruses and potexviruses in arabidopsis and nicotiana benthamiana plants. | the inositol requiring enzyme (ire1) is an endoplasmic reticulum (er) stress sensor. when activated, it splices the bzip60 mrna, producing a truncated transcription factor that upregulates genes involved in the unfolded protein response. bax inhibitor 1 (bi-1) is another er stress sensor that regulates cell death in response to environmental assaults. the potyvirus 6k2 and potexvirus tgb3 proteins are known to reside in the er, serving, respectively, as anchors for the viral replicase and moveme ... | 2016 | 27578623 |
| first report of association of potato virus x and potato virus y and 'candidatus phytoplasma trifolii' in brinjal in india. | symptoms of little leaf, leaf chlorosis and leaf malformations with mosaic mottling symptoms were observed in two brinjal varieties (pusa shyamla and pusa purple cluster) in fields of iari, new delhi, india during 2014-2015. electron microscopy, pcr and sequence analysis first time provided evidence of association of candidatus phytoplasma trifolii with potato virus x and potato virus y in brinjal in india. | 2016 | 27366775 |
| rna-dependent rna polymerase 1 in potato (solanum tuberosum) and its relationship to other plant rna-dependent rna polymerases. | cellular rna-dependent rna polymerases (rdrs) catalyze synthesis of double-stranded rnas that can serve to initiate or amplify rna silencing. arabidopsis thaliana has six rdr genes; rdrs 1, 2 and 6 have roles in anti-viral rna silencing. rdr6 is constitutively expressed but rdr1 expression is elevated following plant treatment with defensive phytohormones. rdr1 also contributes to basal virus resistance. rdr1 has been studied in several species including a. thaliana, tobacco (nicotiana tabacum), ... | 2016 | 26979928 |
| elevated ambient temperature differentially affects virus resistance in two tobacco species. | antiviral defense of plants is usually enhanced by an elevated temperature under natural conditions. in order to better understand this phenomenon, we carried out temperature shift experiments with nicotiana glutinosa plants that were infected with potato virus x (pvx) or the necrotic strain of potato virus y (pvy(n)). the virus titer of the plants was found to be much lower when they were maintained at 30°c compared with 22°c, particularly in the upper leaves. pvx resistance at 30°c persisted f ... | 2016 | 26474332 |
| translationally controlled tumour protein (tctp) from tomato and nicotiana benthamiana is necessary for successful infection by a potyvirus. | translationally controlled tumour protein (tctp) is a ubiquitously distributed protein in eukaryotes, involved in the regulation of several processes, including cell cycle progression, cell growth, stress protection, apoptosis and maintenance of genomic integrity. its expression is induced during the early stages of tomato (solanum lycopersicum) infection by the potyvirus pepper yellow mosaic virus (pepymv, a close relative of potato virus y). tomato tctp is a protein of 168 amino acids, which c ... | 2016 | 27159273 |
| the effects of high temperature on infection by potato virus y, potato virus a, and potato leafroll virus. | we examined the effects of temperature on acquisition of potato virus y-o (pvy-o), potato virus a (pva), and potato leafroll virus (plrv) by myzus persicae by performing transmission tests with aphids that acquired each virus at different temperatures. infection by pvy-o/pva and plrv increased with increasing plant temperature in nicotiana benthamiana and physalis floridana, respectively, after being transmitted by aphids that acquired them within a temperature range of 10-20°c. however, infecti ... | 2016 | 27493607 |
| impact of aphid alarm pheromone release on virus transmission efficiency: when pest control strategy could induce higher virus dispersion. | aphids cause serious damages to crops not only by tacking sap but also by transmitting numerous viruses. to develop biological control, the aphid alarm pheromone, namely e-β-farnesene (eβf), has been demonstrated to be efficient to repel aphids and as attract beneficials, making it a potential tool to control aphid pests. considering aphids also as virus vectors, changes of their behavior could also interfere with the virus acquisition and transmission process. here, a combination of two aphid s ... | 2016 | 27185564 |
| inheritance and effectiveness of two transgenes determining pvy resistance in progeny from crossing independently transformed tobacco lines. | genetic transformation of plants allows us to obtain improved genotypes enriched with the desired traits. however, if transgenic lines were to be used in breeding programs the stability of inserted transgenes is essential. in the present study, we followed the inheritance of transgenes in hybrids originated from crossing two transgenic tobacco lines resistant to potato virus y (pvy): mn 944 lmv with the transgene containing lettuce mosaic virus coat protein gene (lmv cp) and ac gayed roky2 with ... | 2016 | 27878452 |
| the effects of plant virus infection on polarization reflection from leaves. | alteration of leaf surface phenotypes due to virus infection has the potential to affect the likelihood of colonisation by insect vectors, or to affect their feeding activities. the aim of this study was to investigate whether viruses that rely on insects for their transmission, and which can be sensitive to the polarization of light, affect the percentage polarization of light reflected from leaves. we also set out to discover whether a correlation exists between the expression of eceriferum (c ... | 2016 | 27100188 |
| high temperature, high ambient co₂ affect the interactions between three positive-sense rna viruses and a compatible host differentially, but not their silencing suppression efficiencies. | we compared infection of nicotiana benthamiana plants by the positive-sense rna viruses cucumber mosaic virus (cmv), potato virus y (pvy), and by a potato virus x (pvx) vector, the latter either unaltered or expressing the cmv 2b protein or the pvy hcpro suppressors of silencing, at 25°c vs. 30°c, or at standard (~401 parts per million, ppm) vs. elevated (970 ppm) co2 levels. we also assessed the activities of their suppressors of silencing under those conditions. we found that at 30°c, accumula ... | 2015 | 26313753 |
| functional characterization of cucumis metuliferus proteinase inhibitor gene (cmspi) in potyviruses resistance. | proteinase inhibitors are ubiquitous proteins that block the active center or interact allosterically with proteinases and are involved in plant physiological processes and defense responses to biotic and abiotic stresses. the cmspi gene identified from cucumis metuliferus encodes a serine type pi (8 kda) that belongs to potato i type family. to evaluate the effect of silencing cmspi gene on papaya ringspot virus resistance, rna interference (rnai) with an inter-space hairpin rna (ihprna) constr ... | 2015 | 26184285 |
| rna-dependent rna polymerase (nib) of the potyviruses is an avirulence factor for the broad-spectrum resistance gene pvr4 in capsicum annuum cv. cm334. | potyviruses are one of the most destructive viral pathogens of solanaceae plants. in capsicum annuum landrace cm334, a broad-spectrum gene, pvr4 is known to be involved in resistance against multiple potyviruses, including pepper mottle virus (pepmov), pepper severe mosaic virus (pepsmv), and potato virus y (pvy). however, a potyvirus avirulence factor against pvr4 has not been identified. to identify the avirulence factor corresponding to pvr4 in potyviruses, we performed agrobacterium-mediated ... | 2015 | 25760376 |
| snapshot and ce-sscp: two simple and cost-effective methods to reveal genetic variability within a virus species. | the multiplex snapshot and the capillary electrophoresis-single-strand conformation polymorphism (ce-sscp) procedures are here used for rapid and high-throughput description of the molecular variability of viral populations. both approaches are based on (1) standard amplification of genomic sequence(s), (2) labeled primers or labeled single-stranded dna, and (3) migration of fluorescent-labeled molecules in capillary electrophoresis system. the snapshot technology was used to describe the divers ... | 2015 | 25981256 |
| elucidation of virus-host interactions to enhance resistance breeding for control of virus diseases in potato. | potato virus y (pvy) and potato mop-top virus (pmtv) are viruses whose geographical distribution is expanding and economic losses are increasing, in contrast to most of other viruses infecting potato crops. most potato cultivars lack broad-spectrum resistance to the new, genetically complex strains of pvy, and no efficient resistance to pmtv is known in potato. control of the vectors of these viruses is not an efficient or possible strategy to prevent infections. studies on molecular virus-host ... | 2015 | 25931981 |
| rna-seq analysis of resistant and susceptible potato varieties during the early stages of potato virus y infection. | potato virus y (pvy) is one of the most important plant viruses affecting potato production. the interactions between potato and pvy are complex and the outcome of the interactions depends on the potato genotype, the pvy strain, and the environmental conditions. a potato cultivar can induce resistance to a specific pvy strain, yet be susceptible to another. how a single potato cultivar responds to pvy in both compatible and incompatible interactions is not clear. | 2015 | 26091899 |
| interaction between pvy hc-pro and the ntcf1β-subunit reduces the amount of chloroplast atp synthase in virus-infected tobacco. | the photosynthetic rate of virus-infected plants is always reduced. however, the molecular mechanism underlying this phenomenon remains unclear. the helper component-proteinase (hc-pro) of potato virus y (pvy) was found in the chloroplasts of pvy-infected tobacco, indicating some new function of hc-pro in the chloroplasts. we generated hc-pro transgenic plants with a transit peptide to target the protein to chloroplast. the hc-pro transgenic tobacco showed a decreased photosynthetic rate by 25% ... | 2015 | 26499367 |
| a multiplex rt-pcr for simultaneous detection and identification of five viruses and two viroids infecting chrysanthemum. | pathogens causing significant economic losses in chrysanthemum include tomato aspermy virus (tav), chrysanthemum virus b (cvb), cucumber mosaic virus (cmv), tobacco mosaic virus (tmv), potato virus y (pvy), chrysanthemum stunt viroid (csvd) and chrysanthemum chlorotic mottle viroid (cchmvd). a multiplex reverse transcription polymerase chain reaction (rt-pcr) method, using specific primer sets for each virus or viroid, was developed for simultaneous detection and differentiation of tav, cvb, cmv ... | 2015 | 25698104 |
| increased sensitivity of rt-pcr for potato virus y detection using rna isolated by a procedure with differential centrifugation. | the sensitivity of reverse transcription-polymerase chain reaction (rt-pcr) for virus detection is influenced by many factors such as specificity of primers and quality of templates. these factors become extremely important for successful detection when virus concentration is low. total rna isolated from potato virus y (pvy)-infected potato plants using the sodium sulfite rna isolation method or rneasy plant mini kit contains a high proportion of host rna and may also contain trace amount of phe ... | 2015 | 26210699 |
| landing preference and reproduction of rhopalosiphum padi (hemiptera: aphididae) in the laboratory on three maize, potato, and wheat cultivars. | the bird cherry-oat aphid rhopalosiphum padi (l.) transmits the nonpersistent potato virus y (pvy) to seed potatoes. planting a nonvirus host plant around the main crop can reduce pvy incidence, because aphids tend to land in high numbers at the edge of a field and the crop border acts as a virus sink. this study determined r. padi landing and settling preferences and reproductive rates on three cultivars each of maize and wheat compared with potato in the laboratory as a basis for identifying a ... | 2015 | 26022628 |
| stopped in its tracks: how λ-cyhalothrin can break the aphid transmission of a potato potyvirus. | pyrethroids are one of the most widespread and commonly used classes of insecticide and are used in multiple roles, including protecting potato crops from virus vector aphids. resistance in some genotypes of a few species is now widespread, but most species remain susceptible. the rate of virus transmission by two genotypes of the peach potato aphid, myzus persicae, fed on potato virus y (pvy)-infected leaves of potato treated with the pyrethroid λ-cyhalothrin was evaluated. | 2015 | 25523839 |
| effect of insecticide-treated potato plants on aphid behavior and potato virus y acquisition. | the objective was to assess the effect of two contact insecticides, lambda-cyhalothrin and flonicamid, and three systemic insecticides, pymetrozine, dimethoate and imidacloprid, on the behavior and potato virus y (pvy) acquisition of three aphid species, macrosiphum euphorbiae (thomas), rhopalosiphum padi l. and aphis fabae (scopoli). | 2015 | 25159012 |
| variability of potato virus y in tomato crops in poland and development of a reverse-transcription loop-mediated isothermal amplification method for virus detection. | a collection of 147 potato virus y (pvy) isolates from tomato, originating from several commercial fields and greenhouses in different regions of poland, was tested for the presence of pvy by reverse-transcription polymerase chain reaction. however, in some cases, the results obtained were ambiguous. therefore, a sensitive reverse-transcription loop-mediated isothermal amplification method was developed for rapid detection of pvy isolates. phylogenetic and recombination analyses were performed b ... | 2015 | 25961337 |
| recombination of strain o segments to hcpro-encoding sequence of strain n of potato virus y modulates necrosis induced in tobacco and in potatoes carrying resistance genes ny or nc. | hypersensitive resistance (hr) to strains o and c of potato virus y (pvy, genus potyvirus) is conferred by potato genes ny(tbr) and nc(tbr), respectively; however, pvy n strains overcome these resistance genes. the viral helper component proteinases (hcpro, 456 amino acids) from pvy(n) and pvy(o) are distinguished by an eight-amino-acid signature sequence, causing hcpro to fold into alternative conformations. substitution of only two residues (k269r and r270k) of the eight-amino-acid signature i ... | 2015 | 25557768 |
| fluorescently tagged potato virus y: a versatile tool for functional analysis of plant-virus interactions. | potato virus y (pvy) is an economically important plant virus that infects solanaceous crops such as tobacco and potato. to date, studies into the localization and movement of pvy in plants have been limited to detection of viral rna or proteins ex vivo. here, a pvy n605 isolate was tagged with green fluorescent protein (gfp), characterized and used for in vivo tracking. in nicotiana tabacum cv. xanthi, pvy n605-gfp was biologically comparable to nontagged pvy n605, stable through three plant-to ... | 2015 | 25761209 |
| helper component-proteinase enhances the activity of 1-deoxy-d-xylulose-5-phosphate synthase and promotes the biosynthesis of plastidic isoprenoids in potato virus y-infected tobacco. | virus-infected plants show strong morphological and physiological alterations. many physiological processes in chloroplast are affected, including the plastidic isoprenoid biosynthetic pathway [the 2c-methyl-d-erythritol-4-phosphate (mep) pathway]; indeed, isoprenoid contents have been demonstrated to be altered in virus-infected plants. in this study, we found that the levels of photosynthetic pigments and abscisic acid (aba) were altered in potato virus y (pvy)-infected tobacco. using yeast tw ... | 2015 | 25736930 |
| host recovery and reduced virus level in the upper leaves after potato virus y infection occur in tobacco and tomato but not in potato plants. | in this study, the recovery phenomenon following infection with potato virus y (pvy) was investigated in tobacco (nicotiana tobaccum), tomato (solanum lycopersicum) and potato (solanum tuberosum) plants. in tobacco plants, infection of severe strains of pvy (pvyn or pvyn:o) induced conspicuous vein clearing and leaf deformation in the first three leaves above the inoculated leaves, but much milder symptoms in the upper leaves. the recovery phenotype was not obvious in tobacco plants infected wit ... | 2015 | 25679498 |
| potato virus y hc-pro reduces the atpase activity of ntmind, which results in enlarged chloroplasts in hc-pro transgenic tobacco. | potato virus y (pvy) is an important plant virus and causes great losses every year. viral infection often leads to abnormal chloroplasts. the first step of chloroplast division is the formation of ftsz ring (z-ring), and the placement of z-ring is coordinated by the min system in both bacteria and plants. in our lab, the helper-component proteinase (hc-pro) of pvy was previously found to interact with the chloroplast division protein ntmind through a yeast two-hybrid screening assay and a bimol ... | 2015 | 26309250 |
| bimodal dynamics of primary metabolism-related responses in tolerant potato-potato virus y interaction. | potato virus y (pvy) is a major pathogen that causes substantial economic losses in worldwide potato production. different potato cultivars differ in resistance to pvy, from severe susceptibility, through tolerance, to complete resistance. the aim of this study was to better define the mechanisms underlying tolerant responses of potato to infection by the particularly aggressive pvy(ntn) strain. we focused on the dynamics of the primary metabolism-related processes during pvy(ntn) infection. | 2015 | 26386579 |
| rt-pcr and real-time rt-pcr methods for the detection of potato virus y in potato leaves and tubers. | potato virus y (pvy) is a major threat to potato crops around the world. it is an rna virus of the family potyviridae, exhibiting many different strains that cause a range of symptoms in potato. elisa detection of viral proteins has traditionally been used to quantify virus incidence in a crop or seed lot. elisa, however, cannot reliably detect the virus directly in dormant tubers, requiring several weeks of sprouting tubers to produce detectable levels of virus. nor can elisa fully discriminate ... | 2015 | 25287492 |
| induction of resistance to potato virus y strain ntn in potato plants through rnai. | potato viruses cause enormous economic loss in agriculture production. potatoes can be infected by a number of different viruses that affect negatively the harvest and the tuber quality. direct and effective drugs against plant virus diseases are still not available and control is only applied as agricultural measures and pesticides against virus vectors. potato virus y (pvy) is transmitted by aphids in non-persistent manner and on that account using insecticides to prevent spread of the infecti ... | 2015 | 26019615 |
| detection and characterization of viral species/subspecies using isothermal recombinase polymerase amplification (rpa) assays. | numerous molecular-based detection protocols include an amplification step of the targeted nucleic acids. this step is important to reach the expected sensitive detection of pathogens in diagnostic procedures. amplifications of nucleic acid sequences are generally performed, in the presence of appropriate primers, using thermocyclers. however, the time requested to amplify molecular targets and the cost of the thermocycler machines could impair the use of these methods in routine diagnostics. re ... | 2015 | 25981257 |
| immunocapture-multiplex rt-pcr for the simultaneous detection and identification of plant viruses and their strains: study case, potato virus y (pvy). | immunocapture-reverse transcription-polymerase chain reaction (ic-rt-pcr) is a sensitive, reproducible, and robust method for the detection and identification of rna viruses. the ic step provides a simple method to isolate virus particles from plant tissue, particularly when inhibitory substances are present, and thus enables subsequent use of rt-pcr amplification for large-scale virus testing and typing. the multiplex format of the pcr is often used for the detection and identification of multi ... | 2015 | 25981255 |
| tobacco susceptibility to potato virus y(ntn) infection is affected by grafting and endogenous cytokinin content. | faster or stronger response to pathogen occurs if plants undergo prior priming. cytokinins seem to be also involved in plant priming and in response to pathogens. susceptibility to potato virus y(ntn) (pvy(ntn)) was studied in transgenic cytokinin overproducing (pssu-ipt) tobacco and compared with nontransgenic plants. since cytokinin overproduction inhibits development of plant roots and grafting overcomes this limitation, both types were grown as rooted and/or grafted plants to check also the ... | 2015 | 25900563 |
| [comparative analysis of population genetic structure of potato virus y from different hosts]. | nucleotide sequences of p3 and pipo genes of potato virus y (pvy) from potato and tobacco were compared to investigate the effect of hosts on the population genetic structure. meanwhile, mutation, natural selection and gene flow were evaluated to determine evolutionary forces responsible for the population genetic dynamics. the fixation indices of population differentiation (fst) of pvy from tobacco and potato were 0.116 and 0.120, respectively with significant difference, suggesting a moderate ... | 2015 | 25787004 |
| deep sequencing of virus-derived small interfering rnas and rna from viral particles shows highly similar mutational landscapes of a plant virus population. | rna viruses exist within a host as a population of mutant sequences, often referred to as quasispecies. within a host, sequences of rna viruses constitute several distinct but interconnected pools, such as rna packed in viral particles, double-stranded rna, and virus-derived small interfering rnas. we aimed to test if the same representation of within-host viral population structure could be obtained by sequencing different viral sequence pools. using ultradeep illumina sequencing, the diversity ... | 2015 | 25673712 |
| surface plasmon resonance for monitoring the interaction of potato virus y with monoclonal antibodies. | surface plasmon resonance (spr)-based biosensors have been widely utilized for measuring interactions of a variety of molecules. fewer examples include higher biological entities such as bacteria and viruses, and even fewer deal with plant viruses. here, we describe the optimization of an spr sensor chip for evaluation of the interaction of the economically relevant filamentous potato virus y (pvy) with monoclonal antibodies. different virus isolates were efficiently and stably bound to a previo ... | 2014 | 24220292 |
| effects of the sequence characteristics of mirnas on multi-viral resistance mediated by single amirnas in transgenic tobacco. | artificial microrna (amirna) has become the preferred viral defence that can be induced in plants. in this study, nine amirna target sites were selected that were based on the sequence characteristics of natural mirnas in the cylindrical inclusion protein (ci), nuclear inclusion a protein (nia), nuclear inclusion b protein (nib), and coat protein (cp) genes of potato virus y (pvy(n)). these amirnas that exhibited high similarities to the sequences of pvy(n) and tev-sd1 were considered. to study ... | 2014 | 24561715 |
| development of reverse transcription loop-mediated isothermal amplification assay for rapid detection of an emerging potyvirus: tomato necrotic stunt virus. | tomato necrotic stunt virus (tonstv) is an emerging potyvirus that causes severe stunting to infected tomato plants. a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for sensitive detection of tonstv. the sensitivity of rt-lamp was comparable to that of conventional rt-pcr, with detection of tonstv in a reaction containing only 8 pg of total tomato rna or with 1:20,000 dilution of crude tissue extract. this assay was able to detect tonstv in a broad ra ... | 2014 | 24503040 |
| salicylic acid is an indispensable component of the ny-1 resistance-gene-mediated response against potato virus y infection in potato. | the purpose of the study was to investigate the role of salicylic acid (sa) signalling in ny-1-mediated hypersensitive resistance (hr) of potato (solanum tuberosum l.) to potato virus y (pvy). the responses of the ny-1 allele in the rywal potato cultivar and transgenic nahg-rywal potato plants that do not accumulate sa were characterized at the cytological, biochemical, transcriptome, and proteome levels. analysis of noninoculated and inoculated leaves revealed that hr lesions started to develop ... | 2014 | 24420577 |
| molecular and biological characterization of a recombinant isolate of potato virus y from mexico. | an isolate of potato virus y (pvy), pvy-m3, was subjected to biological characterization on potato indicators and to whole-genome sequencing. pvy-m3 induced a local and systemic hypersensitive resistance (hr) response in potato cultivar maris bard expressing the nz gene while inducing no hr in potato cultivars desiree and king edward, carrying ny and nc genes, respectively. these hr responses, combined with a lack of vein necrosis in tobacco, clearly defined pvy-m3 as an isolate of the pvy(z) st ... | 2014 | 24402633 |
| potato virus y infection hinders potato defence response and renders plants more vulnerable to colorado potato beetle attack. | in the field, plants are challenged by more than one biotic stressor at the same time. in this study, the molecular interactions between potato (solanum tuberosum l.), colorado potato beetle (leptinotarsa decemlineata say; cpb) and potato virus y(ntn) (pvy(ntn) ) were investigated through analyses of gene expression in the potato leaves and the gut of the cpb larvae, and of the release of potato volatile compounds. cpb larval growth was enhanced when reared on secondary pvy(ntn) -infected plants ... | 2014 | 25251011 |
| sequence variation of p1 gene in potato virus y isolated from fujian province. | to understand the sequence variation and the putative protein structure of p1 gene in potato virus y (pvy) and to identify the sources of the variation, p1 gene in pvy isolated from fujian province was amplified by reverse-transcription polymerase chain reaction (rt-pcr) using a pair of degenerate primers designed from the conserved regions of published sequences. sequence variation and putative protein structure were analyzed, and phylogenetic tree was reconstructed using bayesian inference met ... | 2014 | 25076037 |
| comparative analysis of virus-specific small rna profiles of three biologically distinct strains of potato virus y in infected potato (solanum tuberosum) cv. russet burbank. | deep sequencing technology has enabled the analysis of small rna profiles of virus-infected plants and could provide insights into virus-host interactions. potato virus y is an economically important viral pathogen of potato worldwide. in this study, we investigated the nature and relative levels of virus-derived small interfering rnas (vsirnas) in potato cv. russet burbank infected with three biologically distinct and economically important strains of pvy, the ordinary strain (pvy-o), tobacco v ... | 2014 | 25036885 |
| differential identification of three species of curtovirus using loop-mediated isothermal amplification. | rapid and sensitive detection methods for three species of curtovirus were developed using a loop-mediated isothermal amplification (lamp) technique. a universal primer set for detecting the three main species of curtovirus at the same time, and three kinds of species-specific primer sets were designed and used for lamp reactions. results from the lamp reactions were visualized both by color changes after adding sybr green i staining dye and by dna laddering on agarose gel electrophoresis. the o ... | 2014 | 24957721 |
| interaction patterns between potato virus y and eif4e-mediated recessive resistance in the solanaceae. | the structural pattern of infectivity matrices, which contains infection data resulting from inoculations of a set of hosts by a set of parasites, is a key parameter for our understanding of biological interactions and their evolution. this pattern determines the evolution of parasite pathogenicity and host resistance, the spatiotemporal distribution of host and parasite genotypes, and the efficiency of disease control strategies. two major patterns have been proposed for plant-virus genotype in ... | 2014 | 24942572 |