Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| stable protein expression in mammalian cells using baculoviruses. | the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) has been widely used in biotechnology for protein expression in insect cells. baculoviruses use arthropods as their natural hosts and are unable to replicate in mammalian cells. however, acmnpv is able to enter many mammalian cell types and can be used for transgene expression if engineered to contain suitable expression cassettes. in this chapter, we describe the construction and application of a recombinant bac ... | 2012 | 21987248 |
| development of a novel baculovirus titration method using the enzyme-linked immunosorbent spot (elispot) assay. | the baculovirus expression vector system (bevs) is one of the most powerful methods for production of recombinant proteins for research or commercial purposes. titration of viable virus in insect cell culture is often required when bevs is used for basic research or bioprocessing. an enzyme-linked immunosorbent spot (elispot) assay using monoclonal antibodies against the major capsid protein vp39 of both autographa californica nuclear polyhedrosis virus (acmnpv) and bombyx mori nuclear polyhedro ... | 2012 | 23274754 |
| baculovirus capsid display potentiates ova cytotoxic and innate immune responses. | baculoviruses (bv) are dna viruses that are pathogenic for insects. although bv infect a range of mammalian cell types, they do not replicate in these cells. indeed, the potential effects of these insect viruses on the immune responses of mammals are only just beginning to be studied. we show in this paper that a recombinant autographa californica multiple nuclear polyhedrosis virus carrying a fragment of ovalbumin (ova) on the vp39 capsid protein (bv-ova) has the capacity to act as an adjuvant ... | 2011 | 21918683 |
| modulation of innate immunity in chickens induced by in vivo administration of baculovirus. | baculoviruses stimulate cytokine production in mammalian cells. they induce a strong innate immune response in animals and have adjuvant properties. the purpose of this work was to study the in vivo effect of baculovirus on chicken innate immune response. spf chickens were inoculated intravenously with autographa californica nuclear polyhedrosis virus (bv). three hours later, chickens were bled, euthanized and their spleen, duodenum and cecal tonsils were excised in order to take samples for rna ... | 2011 | 22142984 |
| [Design of the composition of baculovirus agents]. | The ability of 30 compounds to protect infection bacteria and baculoviruses from the damaging effect of ultraviolet (UV) irradiation was investigated. For this B. thuringiensis var. israelensis spores and gypsy moth (Lymantria dispar L.) nuclear polyhedrosis virus were mixed with different components and exposed to UV irradiation at 0.25 WI cm2 for 60 min. Then spore viability and viral pathogenicity were studied in third instar gypsy moth larvae. The composition comprising sodium alginate, albu ... | 2011 | 22171478 |
| [the search of mirna genes in bombyx mori nuclear polyhedrosis virus genomes regions complementary to the latest genes]. | the search of mirna genes in bombyx mori nuclear polyhedrosis virus genome region complementary to very late genes has been carried out. the search mirna algorithm in silico was developed by us. it was shown that npv b. mori genome region containing orf4 gene complementary to ph gene encodes the potential mirna. npv b. mori genome region containing p74 gene complementary to p10 gene encodes mature mirna and potential mirna. the genome region containing orf1629 encodes two small non-coding rnas c ... | 2011 | 22276429 |
| establishment, growth kinetics, and susceptibility to acmnpv of heat tolerant lepidopteran cell lines. | lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several bombyx cells. they have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. in this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. adaptation of cell lines sf-9, bti-tn-5b1-4 (high5) and bti-tn-mg1 (mg1) to 33-¦c and 35-¦c was carried out by shift ... | 2011 | 21667340 |
| secretory expression of all 16 subtypes of the hemagglutinin 1 protein of influenza a virus in insect cells. | influenza viruses are known for their ability to change their antigenic structure and create new viral strains. hemagglutinin (ha), for which 16 subtypes have been identified, is a major antigenic determinant essential for the pathogenesis of influenza a viruses. to predict and monitor future epidemics, it is critical to produce various ha subtype antigens conveniently and rapidly. a simple, effective, and economic method to generate subunit ha1 of all 16 ha subtypes as recombinant proteins (rha ... | 2011 | 21827791 |
| [the search of microrna genes encoded in antiparallel chains of autographa californica nuclear polyhedrosis virus genome regions, including most late and complementary genes]. | it was shown using bioinformatic approach by analysis of alternative transcriptes secondary structure, that a. californica nuclear polyhedrosis virus gene ph encoded two mature mirnas and three potential mirnas. gene orf1629 complementary to gene ph did not encode mirnas and pre-mirna-cs. gene p10 encodes mature and potential mirna. gene p74 located on complementary chain encodes three mature mirnas. | 2011 | 22145408 |
| expression of ureb and hspa of helicobacter pylori in silkworm pupae and identification of its immunogenicity. | for mass production of urease b subunit (ureb) and heat shock protein a subunit (hspa) of helicobacter pylori with bombyx mori nuclear polyhedrosis virus (bmnpv) baculovirus expression system (bes) and to determine whether they could be used as an oral vaccine against h. pylori, besides, to determine the time course of expressed recombinant protein and the optimum acquisition time directly through green fluorescence, hspa and enhanced green fluorescence protein (egfp) genes were cloned into vect ... | 2011 | 20127514 |
| silkworm salivary glands are not susceptible to bombyx mori nuclear polyhedrosis virus. | a nuclear polyhedrosis virus isolated from infected bombyx mori, bmnpv, was used to inoculate silkworms to determine salivary gland cell susceptibility. the salivary gland was removed from infected silkworms at different times post-inoculation and examined by light microscopy. the salivary gland cells did not exhibit any signs of bmnpv infection; however, fat body and tracheal cells, used as positive controls, showed characteristic cytopathological changes caused by bmnpv infection, which confir ... | 2011 | 21365549 |
| expression from baculovirus and serological reactivity of the nucleocapsid protein of dolphin morbillivirus. | the nucleocapsid (n) protein of dolphin morbillivirus (dmv) was expressed from a baculovirus (autographa californica nuclear polyhedrosis virus) vector and shown by sds-page and western blot analysis to be about 57 kda. transmission electron microscopy revealed fully assembled nucleocapsid-like particles (nlps) exhibiting the typical helical herringbone morphology. these nlps were approximately 20-22 nm in diameter and varied in length from 50 to 100 nm. purified dmv-n protein was used as antige ... | 2010 | 20005643 |
| prorenin processing enzyme (ppe) produced by baculovirus-infected sf-9 insect cells: ppe is the cysteine protease encoded in the acmnpv gene. | in infection cultures of spodoptera frugiperda (sf-9) insect cells with a recombinant baculovirus, vhpr, carrying human preprorenin cdna in the polyhedrin locus of autographa californica multiple nuclear polyhedrosis virus (acmnpv), the expressed inactive recombinant human (rh)-prorenin is reported to be proteolytically processed to yield active rh-renin in the very late phase of culture (takahashi et al., biosci. biotechnol. biochem., 71, 2610-2613 (2007)). to identify the enzyme that catalyzes ... | 2010 | 20139610 |
| phenotypic and genetic characteristic of nuclear polyhedrosis virus (npv) isolates from gypsy moth (lymantria dispar l.) larvae in natural populations of western siberia. | 2010 | 20380159 | |
| host innate immune responses induced by baculovirus in mammals. | the baculovirus autographa californica nuclear polyhedrosis virus has been widely used not only to achieve a high level of foreign gene expression in insect cells but also for efficient gene transduction into mammalian cells without any replication. in addition to the efficient gene delivery, baculovirus has been shown to induce host innate immune responses in various mammalian cells and in mice. the baculovirus has abundant cpg motifs in the viral genome and is capable of inducing pro-inflammat ... | 2010 | 20394574 |
| baculovirus for eukaryotic protein display. | the baculovirus autographa californica nuclear polyhedrosis virus is an attractive candidate for eukaryotic virus display. a variety of strategies exists to incorporate and present target proteins on the surface of infected insect cells as well as on budded virions. native baculovirus proteins such as the major envelope protein and the capsid protein, but also foreign scaffolds such as the vesicular stomatitis virus g-1 protein or the influenza a virus hemagglutinin serve as fusion partners for ... | 2010 | 20433416 |
| [integration of influenza a virus nsp gene into baculovirus genome and its expression in insect cells]. | segment ns in all human influenza a viruses and in the part of avian and animal ones was found to contain an additional (beside ns1 and nep) long open reading frame (orf) enabling to code a polypeptide of 18-26 kd in different strains. this orf, in contrast to the ns1 and nep, locates in positive sense orientation in the negative polarity genomic ns rna segment and the encoded protein is referred nsp (negative strand protein). here, the nsp gene of human influenza a/wsn/33 (h1n1) virus was inser ... | 2010 | 20455463 |
| [phenotypic and genetic characterization of nuclear polyhedrosis virus isolated from gypsy moth (lymantria dispar l.) larvae from the natural populations of western siberia]. | six nuclear polyhedrosis virus (npv) isolates have been isolated from dead larvae of gypsy moth in western siberia. heterogeneity of virulence and reproduction activity was revealed for the npv isolated by bioassay with lymantria dispar l. larvae. the findings may suggest phenotypic variation of the npv isolates. no correlation was found between virulence and reproductive activity with the only exception--the isolate karassuk with a high virulence and a high reproductive activity. nucleotide seq ... | 2010 | 20455470 |
| involvement of tlr21 in baculovirus-induced interleukin-12 gene expression in avian macrophage-like cell line hd11. | our previous work showed that baculovirus (antheraea pernyi nuclear polyhedrosis virus [apnpv]) induced a strong innate immunity and protected chicken from a lethal challenge of bronchitis virus. the purpose of present study was to determine the chicken toll-like receptors (tlrs) used by bv-infected immune cells to induce this immune response. we first investigated the expression of the toll-like receptor (tlr) genes in resting and bv-infected hd11 (chicken macrophage-like cell line) and dt40 (c ... | 2010 | 20471186 |
| baculovirus treatment fully protects mice against a lethal challenge of fmdv. | foot-and-mouth disease virus (fmdv) causes a highly contagious and economically devastating disease that affects cattle, swine, goat and sheep among others. fmdv is able to overcome the initial host innate immune response by inhibiting the induction of antiviral molecules at both the transcriptional and the translational levels. it has been demonstrated that fmdv a/arg/2001 causes the death of adult c57bl/6 mice within 72h. we evaluated the capacity of autographa californica nuclear polyhedrosis ... | 2010 | 20580746 |
| protective effect of partially purified 35 kda protein from silk worm (bombyx mori) fecal matter against carbon tetrachloride induced hepatotoxicity and in vitro anti-viral properties. | it has been found that many proteins from silkworm (bombyx mori l.) fecal matter have been active against human immunodeficiency virus, sendai virus, herpes simplex virus type-1, and nuclear polyhedrosis virus. | 2010 | 20738178 |
| inorganic binding peptide-mediated immobilization based on baculovirus surface display system. | the biomolecule-mediated assembly of novel composites has been the subject of numerous investigations during the last years, providing new insights into material science and engineering. via molecular biology technology, we were able to introduce the genetically engineered polypeptide for inorganics (gepi) as a molecular binder into biomolecules such as phage viruses, to assemble hybrid functional nanoarchitectures. in the present work, we introduced a novel nanocomposite comprising the autograp ... | 2010 | 20806244 |
| high-level expression and improved folding of proteins by using the vp39 late promoter enhanced with homologous dna regions. | some recombinant proteins expressed by baculovirus expression vector systems (bevs) aggregate because the bevs can produce large amounts of protein late during infection, when post-translational modification and protein quality control mechanisms are inactive. for expression during earlier stages than that driven by the polyhedrin (polh) very late promoter, transfer vectors were generated in which this promoter was replaced with a green fluorescent protein (gfp) gene controlled by a vp39 late pr ... | 2010 | 20821250 |
| molecular cloning and characterization of twist gene in bombyx mori. | the twist genes are an evolutionarily conserved group of regulatory basic helix-loop-helix (bhlh) transcription factors. in present study, the twist gene was firstly cloned from bombyx mori and was designated as bmtwist. sequence analysis showed that bmtwist cdna contains a 798 bp open reading frame, encoding a peptide of 266 amino acid residues. sequence alignment showed that bmtwist protein shared extensive homology with other invertebrate twist proteins in bhlh motif. rt-pcr and western blot ... | 2010 | 21076855 |
| nanoparticle surface as activation site. | the immense surface-to-volume (s/v) ratio in nanoparticles leads to large surface energy density. these high densities play the role of sites for activities that are not triggered in bulk materials. here we present some examples of such distinctive activities taking place at nanoparticle surfaces. our first example involves the morphological changes in silkworm (bombyx mori l.) nuclear polyhedrosis virus (bmnpv) brought about by lipophilic amorphous silica nanoparticles (lasn). microscopy studie ... | 2010 | 21137870 |
| [small rnas in bombyx mori nuclear polyhedrosis virus polyhedra form complexes with polypeptide p14 and polyhedrin]. | it was shown that two small rnas about 65 and 55 nucleotides long included in npv b. mori polyhedra form with polypeptides p29 and p14 specific rnp-complexes with molecular weights of 50 and 31 kda, respectively. both complexes form high-molecular weight complex with polyhedrin. origin and nature of p29 and p14 polypeptides are discussed. | 2010 | 21805866 |
| effects of insect viruses and pesticides on glutathione s-transferase activity and gene expression in bombyx mori. | glutathione s-transferase (gst) is a gene family generally associated with detoxification and plays an important role in detoxifying exogenous compounds. the silkworm bombyx mori is an important economic animal for silk production. however, it is liable to infection by a number of viruses and chemical agents that can contaminate its food and growing environment. here we conducted a comparative study of strain- and tissue-specific expressions of gst in the silkworm under infections by bombyx mori ... | 2009 | 19736773 |
| inhibition of influenza virus by baculovirus-mediated shrna. | influenza viruses a and b cause widespread infections of the human respiratory tract; however, existing vaccines and drug therapy are of limited value for their treatment. here we show that bispecific short hairpin small-interfering rna constructs containing an eight-nucleotide intervening spacer, targeted against influenza virus a or influenza virus b, can inhibit the production of both types of virus in infected cell lines. this multiple vector showed remarkable ability to cope with both influ ... | 2009 | 19749373 |
| baculovirus-mediated bispecific short-hairpin small-interfering rnas have remarkable ability to cope with both influenza viruses a and b. | influenza viruses a and b cause widespread infections of the human respiratory tract; however, existing vaccines and drug therapy are of limited value for their treatment. here, we show that bispecific short-hairpin small-interfering rna constructs containing an 8-nucleotide intervening spacer, targeted against influenza virus a or influenza virus b, can inhibit the production of both types of virus in infected cell lines. this multiple vector showed remarkable ability to cope with both influenz ... | 2009 | 19899951 |
| recombinant proteoliposomes prepared using baculovirus expression systems. | proteoliposomes are useful for investigating the functions and properties of membrane proteins. we have established a novel method to prepare proteoliposomes using a baculovirus (autographa californica nuclear polyhedrosis virus; acnpv) gene expression system producing the recombinant membrane proteins to be reconstituted. this method consists of two key steps for production of recombinant proteoliposomes: (1) the cdna of the recombinant membrane protein on a baculovirus vector is transfected in ... | 2009 | 19913163 |
| nanoparticle-virus complex shows enhanced immunological effect against baculovirus. | insects protect themselves from majority of infections by a non-specific innate immune system (present in both vertebrates and invertebrates). bombyx mori nuclear polyhedrosis virus (bmnpv), a baculovirus, causing the deadly grasserie disease is a scourge to silkworm industry and we report here the first success in combating this disease with the help of a nanosilica-virus complex. hydrophobic aluminium silicate nanoparticles were mixed with live bmnpv in vitro. this mixture was injected into on ... | 2009 | 19928266 |
| a novel way to purify recombinant baculoviruses by using bacmid. | in the present study, we studied the feasibility of deleting essential genes in insect cells by using bacmid and purifying recombinant bacmid in escherichia coli dh10b cells. to disrupt the orf4 (open reading frame 4) gene of bmnpv [bm (bombyx mori) nuclear polyhedrosis virus], a transfer vector was constructed and co-transfected with bmnpv bacmid into bm cells. three passages of viruses were carried out in bm cells, followed by one round of purification. subsequently, bacmid dna was extracted a ... | 2009 | 18684105 |
| surface functionalized amorphous nanosilica and microsilica with nanopores as promising tools in biomedicine. | cellular interactions with engineered nanoparticles (nps) are dependent on many properties, inherent to the nanoparticle (viz. size, shape, surface characteristics, degradation, agglomeration/dispersal, and charge, etc.). modification of the surface reactivity via surface functionalization of the nanoparticles to be targeted seems to be important. utilization of different surface functionalization methods of nanoparticles is an emerging field of basic and applied nanotechnology. it is well known ... | 2009 | 18791695 |
| establishment of tetracycline-inducible gene expression systems in the silkworm, bombyx mori. | tetracycline-inducible gene expression (tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. however, the tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, bombyx mori. to construct a functional tet-on system in b. mori, we modified rtta by introducing a transcription activation domain of immediate-early gene 1 of autographa californica nuclear polyhedrosis virus ... | 2009 | 19066730 |
| cloning and expression of simian rotavirus spike protein (vp4) in insect cells by baculovirus expression system. | vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. | 2009 | 19252673 |
| biophysical characterization and unfolding of lef4 factor of rna polymerase from acnpv. | late expression factor 4 (lef4) is one of the four subunits of autographa californica nuclear polyhedrosis virus (acnpv) rna polymerase. lef4 was overexpressed in escherichia coli and recombinant protein was subjected to structural characterization. chemical induced unfolding of lef4 was investigated using intrinsic fluorescence, hydrophobic dye binding, fluorescence quenching, and circular dichroism (cd) techniques. the unfolding of lef4 was found to be a non-two state, biphasic transition. int ... | 2009 | 19274720 |
| an inducible system for highly efficient production of recombinant adeno-associated virus (raav) vectors in insect sf9 cells. | production of clinical-grade gene therapy vectors for human trials remains a major hurdle in advancing cures for a number of otherwise incurable diseases. we describe a system based on a stably transformed insect cell lines harboring helper genes required for vector production. integrated genes remain silent until the cell is infected with a single baculovirus expression vector (bev). the induction of expression results from a combination of the amplification of integrated resident genes (up to ... | 2009 | 19279219 |
| upregulation of proinflammatory cytokines and no production in bv-activated avian macrophage-like cell line (hd11) requires mapk and nf-kappab pathways. | the budded virus (bv) of antheraea pernyi is a nuclear polyhedrosis virus (apnpv), that belongs to the baculovirus family. it is capable of stimulating innate immune response in avian macrophage-like cells (hd11). in this study, we used the expression of proinflammatory cytokines (ifn-gamma and il-12p40) and production of nitric oxide (no) as indicators to evaluate the involvement of the signaling cascades in the activation of hd11 cells by bv. western blot analysis revealed that erk1/2, p38 map ... | 2009 | 19303463 |
| baculovirus induces type i interferon production through toll-like receptor-dependent and -independent pathways in a cell-type-specific manner. | autographa californica nuclear polyhedrosis virus (acnpv) is a double-stranded-dna virus that is pathogenic to insects. acnpv was shown to induce an innate immune response in mammalian immune cells and to confer protection of mice from lethal viral infection. in this study, we have shown that production of type i interferon (ifn) by acnpv in murine plasmacytoid dendritic cells (pdcs) and non-pdcs, such as peritoneal macrophages and splenic cd11c+ dcs, was mediated by toll-like receptor (tlr)-dep ... | 2009 | 19474102 |
| [the effect of fodder on the susceptibility of gypsy moth (lymantria dispar l.) to nuclear polyhedrosis virus]. | gypsy moth (lymantria dispar l.) growing on different feeding substrates was shown to affect their susceptibility to nuclear polyhedrosis virus (npv). the insects feeding on birch leaves had the lowest sensitivity to npv than those on willow leaves, but the insects growing on pine needles showed the highest susceptibility. the sensitivity of the gypsy moths on willow leaves was higher than that of the gypsy moths on birch leaves and lower than that of those on pine needles. at the same time, it ... | 2009 | 19708555 |
| development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems. | we have developed a novel method for the preparation of 'recombinant proteoliposomes'. membrane proteins were expressed on budded virus (bv) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. first, plasmid dna containing the gene for the thyroid-stimulating hormone receptor (tshr) or the acetylcholine receptor alpha-subunit (achralpha) was co-transfected with wild type virus [autographa californica nuclear polyhedros ... | 2008 | 18836200 |
| transgenic breeding of anti-bombyx mori l. nuclear polyhedrosis virus silkworm bombyx mori. | silkworm strains resistant to bombyx mori l. nuclear polyhedrosis virus were obtained through transgenic experiments. piggybac transposon with an a3 promoter were randomly inserted into the silkworm, driving the enhanced green fluorescent protein (egfp) reporter gene into the silkworm genome. polymerase chain reaction results verified the insertion of the extraneous egfp gene, and fluorescence microscopy showed that the egfp was expressed in the midgut tissue. the morbidity ratio of the nuclear ... | 2008 | 18850052 |
| baculovirus up-regulates antiviral systems and induces protection against infectious bronchitis virus challenge in neonatal chicken. | in this study, the antheraea pernyi nuclear polyhedrosis virus (apnpv), a member of the baculovirus family, is evaluated for its stimulation of chicken peripheral blood mononuclear cells (pbmc) and macrophage cell line hd 11 in vitro, and protection against infectious bronchitis virus (ibv) in neonatal chickens in vivo. this study showed that apnpv significantly enhanced inflammatory cytokine mrna expression in chicken pbmc and hd 11 cells through the clathrin-dependent endocytic and endosomal m ... | 2008 | 18707025 |
| the chitinase a from the baculovirus acmnpv enhances resistance to both fungi and herbivorous pests in tobacco. | biotechnology has allowed the development of novel strategies to obtain plants that are more resistant to pests, fungal pathogens and other agents of biotic stress. the obvious advantages of having genotypes with multiple beneficial traits have recently fostered the development of gene pyramiding strategies, but less attention has been given to the study of genes that can increase resistance to different types of harmful organisms. here we report that a recombinant chitinase a protein of the aut ... | 2008 | 17851776 |
| induction of antitumor acquired immunity by baculovirus autographa californica multiple nuclear polyhedrosis virus infection in mice. | the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) has been studied as a gene therapy vector. here, we demonstrated that acmnpv induces antitumor acquired immunity. these results suggest that acmnpv has the potential to be an efficient virus or tumor therapy agent which induces innate and acquired immunity. | 2008 | 18057182 |
| induction of natural killer cell-dependent antitumor immunity by the autographa californica multiple nuclear polyhedrosis virus. | wild-type autographa californica multiple nuclear polyhedrosis virus (acmnpv) infects a variety of mammalian cell types in vitro, but does not replicate in these cells. we investigated the effects of acmnpv in the induction of the immune response and tumor metastasis in mice. after intravenous injection, acmnpv was taken up by the liver and spleen, and preferentially infected dendritic cells (dcs) and b cells in the spleen; costimulatory molecules cd40, cd80, and cd86 were upregulated in the dcs ... | 2008 | 18059370 |
| the effects of bmnpv on biochemical changes in primary cultures of bombyx mori embryonic tissue. | the effect of bombyx mori nuclear polyhedrosis virus (bmnpv) on biochemical changes of tc-100 medium containing 10% fetal bovine serum (fbs) in embryonic primary cultures of silkworm was investigated. the primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums (diluted with tc-100 medium representing multiplicity of infection (moi) of 0.25, 0.5, and 1). glucose, uric acid, urea, total protein, cholesterol, and alkaline phosphatase were measured in the mediu ... | 2008 | 18288542 |
| acmnpv ac143 (odv-e18) is essential for mediating budded virus production and is the 30th baculovirus core gene. | autographa californica multiple nucleopolyhedrovirus (acmnpv) ac143 (odv-e18) is a late gene that encodes for a predicted 9.6 kda structural protein that locates to the occlusion derived viral envelope and viral induced intranuclear microvesicles [braunagel, s.c., he, h., ramamurthy, p., and summers, m.d. (1996). transcription, translation, and cellular localization of three autographa californica nuclear polyhedrosis virus structural proteins: odv-e18, odv-e35, and odv-ec27. virology 222, 100-1 ... | 2008 | 18328526 |
| baculovirus-mediated interferon alleviates dimethylnitrosamine-induced liver cirrhosis symptoms in a murine model. | the wild-type baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) infects a range of mammalian cell types in vitro but does not replicate in these cells. the current study investigated the in vivo effect of acmnpv in the mouse model of liver cirrhosis induced by the mutagen dimethylnitrosamine. intraperitoneal injection of acmnpv induced an immune response. the baculovirus was taken up by the liver and spleen where it suppressed liver injury and fibrosis through the i ... | 2008 | 18369328 |
| nanosilica-from medicine to pest control. | nanotechnology is a broad interdisciplinary area of research, development, and industrial activity that has been growing rapidly worldwide for the past decade. more ambitious uses of nanoparticles are bioremediation of contaminated environments, controlled release of fragrances, biocides, and antifungals on textiles. silica nanocomposites have received much attention because of its thermal degradation behavior and applications in chromatography, medicine, optics, etc. nanobiotech takes agricultu ... | 2008 | 18438740 |
| quantitative analysis of cytoplasmic actin gene promoter and nuclear polyhedrosis virus immediate-early promoter activities in various tissues of silkworm bombyx mori using recombinant autographa californica nuclear polyhedrosis virus as vector. | cassettes harboring luciferase reporter driven by bombyx mori cytoplasmic actin gene promoter (a3) (671 bp) and b. mori nuclear polyhedrosis virus immediate-early promoter (ie-1) (580 bp) were transferred to the bacmid acdeltaegt to generate the recombinant autographa californica nuclear polyhedrosis viruses, acnpva3luc and acnpvieluc, respectively. recombinant baculoviruses were injected into the hemocoele of newly ecdysed 5th instar larvae. the activities of the a3 and ie-1 promoters in variou ... | 2008 | 18535752 |
| cloning and expression analysis of heat shock cognate 70 gene promoter in tiger shrimp (penaeus monodon). | heat shock cognate 70 (hsc70) functions as a molecular chaperon and plays an important role in protein folding. hsc70 cdna of tiger shrimp (penaeus monodon) was cloned and characterized in our previous study. after shrimps were treated with the 1-hr heat shock, the hsc70 mrna level in hemocytes increased (approximately 8 fold) using real-time quantitative pcr. an hsc70 clone was obtained from genomic library screening. the gene contains 2 exons separated by a 1557-bp intron. the 5'-flanking regi ... | 2007 | 17931801 |
| function and oligomerization study of the leucine zipper-like domain in p13 from leucania separata multiple nuclear polyhedrosis virus. | the p13 gene is uniquely present in group ii nucleopolyhedroviruses (npvs) and some granuloviruses, but not in group i npvs. p13 gene was first described by our laboratory in leucania separatamultiple nuclear polyhedrosis virus (ls-p13) in 1995. however, the functions of ls-p13 and of its homologues are unknown. when ls-p13 was inserted into autographa californica nucleopolyhedrovirus, a group i npv, polyhedra yield was inhibited. however, this inhibition was prevented when the leucine zipper-li ... | 2007 | 17394774 |
| a highly efficient method for the generation of a recombinant bombyx mori nuclear-polyhedrosis-virus bacmid and large-scale expression of foreign proteins in silkworm (b. mori) larvae. | in the post-genomic era, one of the challenges and a source of competition is the development of high-throughput, large-scale and low-cost eukaryotic cdna cloning and expression systems. the baculovirus expression system is the most popular one and plays an important role in the high-level expression of eukaryotic proteins. in the present study, a convenient, rapid and highly efficient method for the construction of recombinant bmnpv (bombyx mori nuclear polyhedrosis virus)-bacmid vector (bmbacm ... | 2007 | 17428194 |
| influence of cytochrome c on apoptosis induced by anagrapha (syngrapha) falcifera multiple nuclear polyhedrosis virus (afmnpv) in insect spodoptera litura cells. | we investigated the influence of cytochrome c on apoptosis induced by anagrapha (syngrapha) falcifera multiple nuclear polyhedrosis virus (afmnpv). microscopic observation revealed that infection of sl-1 cells with afmnpv resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of afmnpv-infected sl-1cells. western blot analysis demonstrated that afmnpv-induced apoptosis in insect sl-1 cells was significantly inhibited by cyclosporin a which blocked a translocation of cyt ... | 2007 | 17478109 |
| purification and characterization of a recombinant rat prohaptoglobin expressed in baculovirus-infected sf9 insect cells. | to generate hemoglobin-free full-length haptoglobin the cdna encoding rat haptoglobin alphabeta subunits was cloned into shuttle vector pvt-bac-his and used to produce a recombinant baculovirus autographa californica nuclear polyhedrosis virus (acnpv) as an expression vector, named hpacnpv. recombinant virus was used to infect spodoptera frugiperda (sf9) insect cells. the 50 kda protein expressed was mostly secreted into the culture medium at relatively high titer (15 microg/ml) and was found to ... | 2007 | 17681809 |
| p13 of leucania separata multiple nuclear polyhedrosis virus affected the polyhedra and budded virions yields of acmnpv. | p13 gene was first described by our laboratory in leucania separata multiple nuclear polyhedrovirus (ls-p13, orf114) back to 1995. however, the functions of ls-p13 and its reported homologues remained unknown. in order to probe the function of ls-p13, recombinant autographa californica nucleopolyhedroviruses (racmnpvs) were constructed to express ls-p13 in the sf9 cells at early, late or early/late phase. observations of microscope showed that the expression of ls-p13 could decrease the yield of ... | 2007 | 17141348 |
| enhanced effect of fluorescent whitening agent on peroral infection for recombinant baculovirus in the host bombyx mori l. | the low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (bombyx mori l). in this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. the results showed that the peroral infection can be remarkably enhanced by adding ... | 2007 | 17160363 |
| using cell size kinetics to determine optimal harvest time for spodoptera frugiperda and trichoplusia ni bti-tn-5b1-4 cells infected with a baculovirus expression vector system expressing enhanced green fluorescent protein. | infecting insect cells with a baculovirus expression vector system (bevs) is an increasingly popular method for the production of recombinant proteins. due to the lytic nature of the system, however, determining the optimal harvest time is critical for maximizing protein yield. we found that measuring the change in average diameter during the progress of infection with an automated cell analysis system (cedex hires, innovatis ag) could be used to determine the time of maximum protein production ... | 2007 | 19003016 |
| optimization of baculovirus transduction on freestyle293 cells for the generation of influenza b/lee/40. | recombinant baculovirus expression vectors derived from the autographa californica nuclear polyhedrosis virus can serve as efficient gene-transfer vehicles for transient expression of recombinant proteins in a wide range of mammalian cell types and are able to produce multisubunit particles such as viruses or virus like particles. in this study, we constructed eight recombinant baculoviruses each containing one of the influenza b/lee/40 virus genes in a bidirectional expression cassette for simu ... | 2006 | 17172661 |
| [improved efficient gene transfer into insect and mammalian cells by baculovirus vectors]. | the fragments of genomics dna of the nuclear polyhedrosis virus (npv) containing genes of late viral proteins p10, p35, p39, were cloned, the promoter regions of this genes were used to design baculovirus transfer vectors. a double-promoter and triple-promoter baculovirus transfer vectors were obtained. recombinant baculovirus vectors containing mammalian expression cassette with cytomegalovirus (cmv) promoter, the gene for green or red fluorescent protein, sv40pa and polylinker mcs were constru ... | 2006 | 17209436 |
| the autographa californica nuclear polyhedrosis virus acnpv induces functional maturation of human monocyte-derived dendritic cells. | the initiation of an adaptive immune response is critically dependent on the activation of dendritic cells (dcs). therefore, vaccination strategies targeting dcs have to ensure a proper presentation of the immunogen as well as an activation of dcs to accomplish their full maturation. viral vectors can achieve gene delivery and a subsequent presentation of the expressed immunogen, however, the immunization efficiency may be hampered by an inhibition of dc activation. here we report that the insec ... | 2006 | 16870312 |
| cloning and characterization of bombyx mori pp-bp a gene induced by viral infection. | the enf peptide family, so termed after the consensus sequence in their amino termini (glu-asn-phe-), is assumed to play multiple important roles in defense reactions, growth regulation, and homeostasis of lepidopteran insects. the paralytic peptide of bombyx mori (bmpp) is one such peptide that is involved in the paralytic and plasmatocyte-spreading activities in the hemocyte immune reaction. the growth-blocking peptide of pseudaletia separata (psgbp), which is also a member of the enf peptide ... | 2006 | 17112968 |
| expression of spider flagelliform silk protein in bombyx mori cell line by a novel bac-to-bac/bmnpv baculovirus expression system. | bombyx mori nuclear polyhedrosis virus (bmnpv) baculovirus expression system (bes) has a lot of advantages such as high expression efficiency, convenience, and low feeding cost. in this report, we used a recently developed bmnpv bacmid, which could infect both b. mori cell lines and silkworm larvae. the results showed it takes only 7 to 10 days to generate recombinant baculovirus and permit the rapid isolation from small-scale cultures and then use it to transfect b. mori cell lines, compared to ... | 2006 | 16158284 |
| the use of a recombinant baculovirus expressing a chitinase from the hard tick haemaphysalis longicornis and its potential application as a bioacaricide for tick control. | baculoviruses are specific insect pathogens used as selective biological insecticides on lepidopteran insects. we have tested a recombinant baculovirus expressing a chitinase gene for its efficacy as a tick bioacaricide. the recombinant autographa californica multiple nuclear polyhedrosis virus expressing a chitinase enzyme (acmnpv-cht1) from the hard tick, haemaphysalis longicornis, was constructed and found to have a novel bioacaricidal effect against ticks. the recombinant baculovirus was use ... | 2006 | 16292677 |
| functional expression of lepidopteran-selective neurotoxin in baculovirus: potential for effective pest management. | recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. recently we identified and characterized a novel lepidopteran-selective toxin, buthus tamulus insect-selective toxin (butait), having 37 amino acids and eight half cysteine residues from the venom of the south indian red scorpion, mesobuthus tamulus. the synthetic toxin gene containing the butait se ... | 2006 | 16406338 |
| in vivo analysis of fibroin heavy chain signal peptide of silkworm bombyx mori using recombinant baculovirus as vector. | in order to investigate the functional signal peptide of silkworm fibroin heavy chain (fibh) and the effect of n- and c-terminal parts of fibh on the secretion of fibh in vivo, n- and c-terminal segments of fibh gene were fused with enhanced green fluorescent protein (egfp) gene. the fused gene was then introduced into silkworm larvae and expressed in silk gland using recombinant acmnpv (autographa californica multiple nuclear polyhedrosis virus) as vector. the fluorescence of egfp was observed ... | 2006 | 16466694 |
| antibody-based surface plasmon resonance detection of intact viral pathogen. | surface plasmon resonance (spr) technique was used to directly detect an intact form of insect pathogen: the baculovirus, autographa californica multiple nuclear polyhedrosis virus (acmnpv). an spr sensor chip with three bio-functional layers was used to detect the intact acmnpv: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, protein a, and a mouse igg monoclonal antibody raised against a surface protein of the target viral pathogen. a two-channel (reference & tes ... | 2006 | 16470580 |
| construction of hybrib autographa californica nuclear polyhedrosis bacmid by modification of p143 helicase. | we developed a new hybrid nuclear polyhedrosis virus (npv) bacmid capable of infecting spodoptera frugiperda, tricoplusia ni, and bombyx mori, and b. mori cell lines for producing hybrid recombinant baculovirus that can carry a gene of interest and express it in a broad range of hosts. a gfp(uv)-beta1,3-n-acetylglucosaminyltransferase 2 fusion gene was expressed successfully in silkworm larvae using this hybrid bacmid. the hybrid npv bacmid provides an altogether simple and realistically feasibl ... | 2006 | 16478634 |
| baculovirus etl promoter acts as a shuttle promoter between insect cells and mammalian cells. | to identify a shuttle promoter that can mediate gene expression in both insect cells and mammalian cells to facilitate the development of a baculovirus vector-based mammalian cell gene delivery vehicle. | 2006 | 16490168 |
| characterization of baculovirus autographa californica multiple nuclear polyhedrosis virus infection in mammalian cells. | the baculovirus autographa californica multiple nuclear polyhedrosis virus (acmnpv) is used as a vector in many gene therapy studies. wild-type acmnpv infects many mammalian cell types in vitro, but does not replicate. we investigated the dynamics of acmnpv genomic dna in infected mammalian cells and used flow cytometric analysis to demonstrate that recombinant baculovirus containing a cytomegalovirus immediate early promoter/enhancer with green fluorescent protein (gfp) expressed high levels of ... | 2006 | 16545777 |
| a novel method for in vitro radiolabeling and testing enveloped viruses by phosphatidylethanolamine n-methyltransferase and host cell-specific binding. | the present study developed a novel virus labeling and testing method, referred to as an envelope-labeled virus assay (elva), in which virus envelope is labeled in vitro by the action of phosphatidylethanolamine n-methyltransferase (pemt) and tested through a host cell-specific binding. a recombinant strain (vgfpuv) of autographa californica multiple nuclear polyhedrosis virus (acmnpv) and spodoptera frugiperda (sf-9) insect cells were used as a model of viruses and host cells, respectively. the ... | 2006 | 16552776 |
| expression of bioactive recombinant bovine interferon-gamma using baculovirus. | the precise role of bovine interferon-gamma (boifn-gamma) in disease and therapy is still poorly defined. clearly it is involved in defence against parasites, bacteria, viruses and possibly tumor cells. this paper reports the expression of boifn-gamma in a baculovirus system to generate a fully functional recombinant protein. bovine interferon-gamma cdna was cloned from mitogen stimulated peripheral blood mononuclear cell (pbmc) rna utilizing the reverse transcription-polymerase chain reaction ( ... | 2006 | 16608121 |
| enhancing insecticidal efficacy of baculovirus by early expressing an insect neurotoxin, lqhit2, in infected trichoplusia ni larvae. | lqhit(2), an insect specific neurotoxin from the venom of leiurus quinquestriatus hebraeus, has been demonstrated to improve insecticidal efficacy of autographa californica nuclear polyhedrosis virus (acmnpv). a polyhedrin-positive recombinant acmnpvvacp(hsp70)egfp/p(pag90)it(2) was engineered for larvae to express the enhanced green fluorescence protein (egfp) and lqhit(2) under the control of p(hsp70) and p(pag90) promoters, respectively. this would allow a visual observation of the viral infe ... | 2006 | 16636828 |
| cross infectivity of baculovirus, spilarctia obliqua nuclear polyhedrosis virus against mulberry pest, porthesia xanthorrhoea kollar. | spilarctia obliqua nucleopolyhedrosis virus (sonpv) has been found effective against s. obliqua (walker) as well as porthesia xanthorrhoea kollar. lc50 concentration of s. obliqua sonpv against s. obliqua and p. xanthorrhoea was 2.5 x 10(4) and 3.7 x 10(4) pobs/ml, respectively. lt50 of sonpv against s. obliqua was 5.73 days, while it was 6.98 days for p. xanthorrhoea. cross infectivity of sonpv against p. xanthorrhoea and ease of its mass production may make it an ideal biopesticide. | 2006 | 16708898 |
| improving baculovirus transduction of mammalian cells by surface display of a rgd-motif. | an rgd-containing peptide, comprising 23 amino acids from the foot-and-mouth disease virus (fmdv) vp1 protein was engineered into the envelope of autographa californica nuclear polyhedrosis virus surface (acnpv) using two different display strategies. the rgd-motif is a well-described tripeptide, that by binding to cell surface integrins facilitates virus entry into cells. this epitope was displayed, either by directly modifying the native major envelope protein gp64 of acnpv, or by incorporatin ... | 2006 | 16716431 |
| effects of cry1ac toxin of bacillus thuringiensis and nuclear polyhedrosis virus of helicoverpa armigera (hübner) (lepidoptera: noctuidae) on larval mortality and pupation. | in the laboratory, the percentage mortality and pupation of helicoverpa armigera (hübner) were investigated when larvae were exposed to cry1ac of bacillus thuringiensis berliner, nuclear polyhedrosis virus of h. armigera (hanpv) or cry1ac and hanpv together. the results revealed that interactions between cry1ac and hanpv varied with bioassay method and concentration of the suspension. when larvae were infected using a suspension containing both hanpv and cry1ac, most combinations of cry1ac (62.5 ... | 2006 | 16770833 |
| loss of posterior silk gland transcription specificity of fibroin light chain promoter due to absence of 41 bp sequence containing possible inhibitor binding sites. | the gene encoding fibroin light chain protein (fibl) is specifically expressed in the posterior silk gland of silkworm and repressed in other tissues. the binding sites of several transcription factors involved in the silk gland transcription specificity of fibl promoter have been recognized, including sgfb, psgf and bmfa. here we report the leak expression of the enhanced green fluorescent protein (egfp) reporter gene in tissues other than the posterior silk gland in vivo when under the control ... | 2005 | 16331326 |
| the murine thymic stromal lymphopoietin receptor is partially expressed with an extracellular c-terminus. | the gene encoding the murine thymic stromal lymphopoietin receptor was expressed in lepidopteran insect cells using the baculovirus expression vector system. the corresponding gene was inserted under the polyhedrin promoter of the autographa californica nuclear polyhedrosis virus and expressed with an n-terminal poly-histidine tag and a c-terminal flag-tag in the spodoptera frugiperda insect cell line sf9 during viral infection. flow cytometer analysis of cells infected with the produced recombi ... | 2005 | 15488943 |
| transformed lepidopteran cells expressing a protein of the silkmoth fat body display enhanced susceptibility to baculovirus infection and produce high titers of budded virus in serum-free media. | baculovirus vectors constitute important tools for therapeutic protein production and mammalian cell transduction for gene therapy applications. a prerequisite for such applications is that the cell lines in which baculoviruses are propagated be maintained in serum-free media that are devoid of potential human pathogens. however, in serum-free media, the performance of baculovirus-based systems can be significantly reduced. in this report, we show that silkmoth-derived host cell lines for the bo ... | 2005 | 16233927 |
| development of a new high-throughput screening model for human high density lipoprotein receptor (cla-1) agonists. | to develop a new high-throughput screening model for human high-density lipoprotein (hdl) receptor (cd36 and limpii analogous-1, cla-1) agonists using cla-1-expressing insect cells. | 2005 | 16274109 |
| preparation of chil-2 and ibdv vp2 fusion protein by baculovirus expression system. | this study aims to produce an effective subunit vaccine against infectious bursal disease virus (ibdv). the genes of chicken interleukin-2 (chil-2) and ibdv viral protein 2 (vp2) were amplified and fused by splice overlap extension-polymerase chain reaction (soe-pcr). the fusion gene was digested by ecor i/kpn i and inserted into pbacpak8 vector, resulting in recombinant transfer plasmid pbacpakvp2-il2. the recombinant plasmid was transfected into sf-9 cells accompanied with hybrid nuclear polyh ... | 2005 | 16212892 |
| insecticidal effects of buthus occitanus tunetanus botit6 toxin expressed in escherichia coli and baculovirus/insect cells. | botit6 is a neurotoxin polypeptide derived from the venom of the scorpion buthus occitanus tunetanus (bot). its mature form is composed of 62 amino acids. botit6 has been reported to be the most potent toxin from bot venom that has a strict selectivity for insects. such toxin may have potential as a potent animal-harmless tool against insects. using rt-pcr, we isolated and sequenced a cdna encoding 62 amino acid residues corresponding to the known amino acid sequence of botit6. we have expressed ... | 2005 | 16216259 |
| efficient large-scale protein production of larvae and pupae of silkworm by bombyx mori nuclear polyhedrosis virus bacmid system. | silkworm is one of the most attractive hosts for large-scale production of eukaryotic proteins as well as recombinant baculoviruses for gene transfer to mammalian cells. the bacmid system of autographa californica nuclear polyhedrosis virus (acnpv) has already been established and widely used. however, the acnpv does not have a potential to infect silkworm. we developed the first practical bombyx mori nuclear polyhedrosis virus bacmid system directly applicable for the protein expression of silk ... | 2005 | 15596136 |
| involvement of the toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus. | we have previously shown that mice inoculated intranasally with a wild-type baculovirus (autographa californica nuclear polyhedrosis virus [acnpv]) are protected from a lethal challenge by influenza virus. however, the precise mechanism of induction of this protective immune response by the acnpv treatment remained unclear. here we show that acnpv activates immune cells via the toll-like receptor 9 (tlr9)/myd88-dependent signaling pathway. the production of inflammatory cytokines was severely re ... | 2005 | 15709004 |
| construction of a transposon-mediated baculovirus vector hanpvid and a new cell line for expressing barnase. | in this study we developed the transposon-mediated shuttle vector 'hanpvid', which composed of hanpv (heliothis armigera nuclear polyhedrosis virus) genomic dna and a transposon cassette from bacmid of bac-to-bac system. hanpvid replicates in e. coli in the same way as bacmid and retains infective function in cotton bollworm cells (hz-am1). using hanpvid we constructed a recombinant virus, which could infect hz-am1 cells and generate recombinant hanpv (rha-bar) containing the barnase gene, a rib ... | 2005 | 15715945 |
| the function of envelope protein p74 from autographa californica multiple nucleopolyhedrovirus in primary infection to host. | this research investigated the function of envelope protein p74 of autographa californica multiple nuclear polyhedrosis virus (acmnpv) in primary infection to host. a p74-inactivation recombinant baculovirus, rac-gfp(delta) p74, was constructed by inserting gfp driven by acmnpv polyhedrin promoter into the p74 locus of acmnpv genome. bioassays showed that the p74-null occlusion bodies (obs) failed to infect its natural host larvae, spodoptera exigua, per os, while the p74-null budded virus (bvs) ... | 2005 | 15744572 |
| cloning and characterization of the g protein betagamma subunits from trichoplusia ni (high five cells). | baculoviral-mediated expression in insect cells has become a method of choice where high-level protein expression is desired and where expression in escherichia coliform (e. coli.) is unsuitable. genes of interest are inserted into the baculoviral genome of autographa californica nuclear polyhedrosis virus (acnpv) under the extremely strong, but very late polyhedron gene (polh). the preferred host lines are derived from spodoptera frugiperda (sf9 or sf21) or tricoplusia ni (high five, invitrogen ... | 2005 | 15763469 |
| microarray and rt-pcr screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp. | here, we report for the first time the successful use of cycloheximide (chx) as an inhibitor to block de novo viral protein synthesis during wssv (white spot syndrome virus) infection. sixty candidate ie (immediate-early) genes were identified using a global analysis microarray technique. rt-pcr showed that the genes corresponding to orf126, orf242 and orf418 in the taiwan isolate were consistently chx-insensitive, and these genes were designated ie1, ie2 and ie3, respectively. the sequences for ... | 2005 | 15780883 |
| baculoviral polyhedrin-bacillus thuringiensis toxin fusion protein: a protein-based bio-insecticide expressed in escherichia coli. | previously, we found that baculoviral polyhedrin (polh) used as a fusion partner for recombinant expression in escherichia coli showed almost the same characteristics (rapid solubilization under alkaline conditions and specific degradation by specific alkaline proteases in insect midgut) as the native baculoviral polh, and formed easily isolatable inclusion bodies. here, polh derived from the autographa californica nuclear polyhedrosis virus (acnpv) was fused with a bacillus thuringiensis (bt) t ... | 2005 | 15981278 |
| fossil evidence of insect pathogens. | the present report describes fossil evidence of insect pathogens, heretofore, almost non-existent, from six samples of amber ranging in age from 15 to 100 million years. they include a cytoplasmic polyhedrosis virus and trypanosomatid infection in an adult biting midge (diptera: ceratopogonidae), and a nuclear polyhedrosis virus in an adult sand fly (diptera: phlebotomidae), both from early cretaceous burmese amber, several types of fungal thalli on the cuticle of an adult mosquito (culicidae: d ... | 2005 | 16009374 |
| genetically engineered horseradish peroxidase for facilitated purification from baculovirus cultures by cation-exchange chromatography. | an engineered horseradish peroxidase isozyme c (hrp c) gene was constructed by the addition of a 6xarg fusion tail to 6xhis-hrp c by the pcr strategy. the 6xhis-6xarg-hrp c cdna was expressed in the sf9 insect cell line from spodoptera frugiperda infected with autographa californica nuclear polyhedrosis virus. the recombinant peroxidase isoelectric point was 9.5 as judged by isoelectric focusing and was purified directly from the culture medium at day-6 post-infection by cation-exchange chromato ... | 2005 | 16038999 |
| from metchnikoff to monsanto and beyond: the path of microbial control. | in 125 years since metchnikoff proposed the use of metarhizium anisopliae to control the wheat cockchafer and brought about the first field trials, microbial control has progressed from the application of naturalists' observations to biotechnology and precision delivery. this review highlights major milestones in its evolution and presents a perspective on its current direction. fungal pathogens, the most eye-catching agents, dominated the early period, but major mycological control efforts for ... | 2005 | 16039302 |
| expression and one-step purification of bovine interleukin-21 (il-21) in silkworms using a hybrid baculovirus expression system. | a hybrid baculovirus, a hybrid of the autographa californica nuclear polyhedrosis virus and the bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of bovine interleukin-21 (il-21) in silkworms. a recombinant hybrid baculovirus containing the full length of the cdna of bovine interleukin-21 was constructed and used to infect silkworm larvae or silkmoth pupae. after the infection of the virus, bovine mature il-21 was produced in the haemolymph or pupal cell lysates. a ... | 2004 | 15604780 |
| construction of a host range-expanded hybrid baculovirus of bmnpv and acnpv, and knockout of cysteinase gene for more efficient expression. | acnpv (autographa californica nuclear polyhedrosis virus) and bmnpv(bombyx mori nuclear polyhedrosis virus) are two principal insect-baculovirus expression systems, each having different characteristics. acnpv has a wider host range and can infect a series of cell lines thus making it suitable for cell suspension culture expression, but the small size of the host insect, a. californica, makes acnpv less suitable for large scale protein synthesis. in contrast, bmnpv can only infect the silkworm, ... | 2004 | 15623152 |
| ha-vp39 binding to actin and the influence of f-actin on assembly of progeny virions. | we present evidence that actin is necessary for the successful assembly of hanpv virions. purified nucleocapsid protein ha-vp39 of heliothis armigera nuclear polyhedrosis virus (hanpv) was found to be able to bind to actin in vitro without assistance, as demonstrated by western blot and isothermal titration calorimeter. deltah and binding constants (k) detected by isothermal titration calorimeter strongly suggested that ha-vp39 first binds actin to seed the formation of hexamer complex of actin, ... | 2004 | 15503206 |
| promoter and piggybac activities within embryos of the potato tuber moth, phthorimaea operculella, zeller (lepidoptera: gelechiidae). | potato production in tropical and subtropical countries suffers from damage caused by the potato tuber moth (ptm), phthorimaea operculella. the aim of this research was the development of the components required for a germline transformation system for the ptm. we tested three components that are critical to genetic transformation systems for insects: promoter activity, marker gene expression, and transposable element function. we compared the transcriptional activities of five different promote ... | 2004 | 15527988 |
| high-level expression of recombinant 3ab1 non-structural protein from fmdv in insect larvae. | for its potential usefulness in diagnosis, the non-structural protein 3ab1 from foot-and-mouth disease virus was expressed as a soluble protein by using autographa californica nuclear polyhedrosis virus as a vector. the 3ab1 coding sequence was introduced into acnpv genome via pbacpak3ab1 transfer vector to originate ac3ab1 recombinant baculovirus of phenotype occ-. rachiplusia nu larvae were injected with supernatants of sf9 cells infected with ac3ab1 and 5 days post-infection total protein ext ... | 2004 | 15664073 |
| baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination. | recombinant baculoviruses derived from the autographa californica nuclear polyhedrosis virus (acnpv) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system (bevs) is hampered by slow and tedious procedures for the selection and separation of baculovirus-infected insect cells and for titer determination. here we developed a new technology based on the bicistronic vector with a fusion protein of the human integral plasma membrane glyc ... | 2004 | 14740489 |
| [cooperative effect of spodoptera litura nuclear polyhedrosis virus with parasite wasp, microplitis sp. on the s. litura larvae]. | the relationship between the survival rate, developmental duration of microplitis sp. in spodoptera litura larvae and time of exposure of the host larvae to virus, the inoculation virus concentration were investigated to determined if parasites which emerged from virus-infected s. litura larvae had normal survive, and if the parasites could sever as the vector for s. litura nuclear polyhedrosis virus (sinpv). there was no significant difference in time that parasites spent in virus-infected larv ... | 2004 | 15146645 |