Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| tissue-specific transcriptomes of anisakis simplex (sensu stricto) and anisakis pegreffii reveal potential molecular mechanisms involved in pathogenicity. | larval stages of the sibling species of parasitic nematodes anisakis simplex (sensu stricto) (s.s.) (as) and anisakis pegreffii (ap) are responsible for a fish-borne zoonosis, known as anisakiasis, that humans aquire via the ingestion of raw or undercooked infected fish or fish-based products. these two species differ in geographical distribution, genetic background and peculiar traits involved in pathogenicity. however, thus far little is known of key molecules potentially involved in host-para ... | 2018 | 29321072 |
| anisakis spp. larvae in different kinds of ready to eat products made of anchovies (engraulis encrasicolus) sold in italian supermarkets. | in this study the occurrence of visible anisakid larvae in semi-preserved anchovy products sold on the italian market was investigated. totally, 107 ready to eat products (33 salted-ripened, 49 in oil and 25 marinated) were sampled. each sample was digested, then the digested material was observed under natural and uv light. parasites were counted, collected and microscopically identified to genus level. a representative subset was molecularly identified using the cox2 gene. at least one visible ... | 2018 | 29306733 |
| metazoan parasite fauna of migrating common garfish, belone belone (l.), in the baltic sea. | a total of 35 common garfish, belone belone (linnaeus, 1761), were studied for metazoan parasites on their spawning grounds in the western baltic sea. nine parasite species were found, and six new locality records could be established for german coastal waters (axine belones, monogenea; proteocephalus sp., cestoda; anisakis simplex (s.s.), contracaecum rudolphii a and hysterothylacium aduncum, nematoda; echinorhynchus gadi, acanthocephala). for the first time, the marine ectoparasite a. belones ... | 2018 | 29351069 |
| detection of anisakid larvae in marinated mackerel sushi in tokyo, japan. | 2017 | 29279440 | |
| prevalence, molecular characterization, and clinical relevance of sensitization to anisakis simplex in children with sensitization and/or allergy to dermatophagoides pteronyssinus. | prevalence of the anisakis simplex's (as) sensitization in children sensitized to dermatophagoides pteronissynus (dp) is not known, neither it is to which percentage it might be due to cross-reactivity. the primary objective of the present retrospective cross-sectional study is to evaluate the prevalence of sensitization to as in children sensitized or allergic to dp. secondary outcomes were the prevalence of cross-reactivity and clinical relevance of the condition. the prevalence of sensitizati ... | 2017 | 29249135 |
| a multicenter study of ige sensitization to anisakis simplex and diet recommendation | allergy to anisakis simplex (s.) is spreading due to the increased consumption of raw, smoked or marinated fish. in humans, anisakis s. can directly attack the gastrointestinal mucosa, provoking a parasitosis known as anisakiasis, or giving rise to the formation of ige and, finally, inducing ige-mediated reactions like urticaria, angioedema and anaphylactic shock. during recent years, a dietary approach to anisakis s. infestation has also been addressed. | 2017 | 29189183 |
| modelling studies determing the mode of action of anthelmintics inhibiting in vitro trehalose-6-phosphate phosphatase (tpp) of anisakis simplex s.l. | the trehalose-6-phosphate phosphatase (tpp) enzyme is involved in the synthesis of trehalose, the main sugar in the energy metabolism of nematodes. tpp is a member of the had-like hydrolase superfamily and shows a robust and specific phosphatase activity for the substrate trehalose-6-phosphate. the presence of conserved active sites of tpp in closely related nematodes and its absence in humans makes it a promising target for antiparasitic drugs. in the present study, homology modeling, molecular ... | 2017 | 29170085 |
| a combined human case of dirofilaria ursi infection in dorsal subcutaneous tissue and anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue. | dirofilaria ursi is a filarial nematode that parasitizes the subcutaneous tissues of the american black bear (ursus americanus) and japanese black bear (ursus thiabetanus japonicus). d. ursi that has parasitized black bears has the potential to subsequently infect humans. in addition, extra-gastrointestinal anisakiasis is less common in japan. | 2017 | 29118653 |
| human gastric hyperinfection by anisakis simplex: a severe and unusual presentation and a brief review. | anisakiasis is an emerging marine food-borne zoonosis resulting from the accidental ingestion of anisakis larvae, through the consumption of raw or undercooked infected seafood products. the first case of human gastric hyperinfection by anisakis simplex with an unusual and severe presentation, occurring in a portuguese woman, is described in this article. over 140 anisakid larvae were removed by gastroscopy. massive infection is uncommon in areas where the consumption of raw fish is not part of ... | 2017 | 28882665 |
| allergenic activity of pseudoterranova decipiens (nematoda: anisakidae) in balb/c mice. | anisakis simplex is the only fishery-product associated parasite causing clinical allergic responses in humans so far. however, other anisakids, due to the presence of shared or own allergens, could also lead to allergic reactions after sensitization. the aim of this study was to determine if pseudoterranova decipiens belonging to the family anisakidae has allergenic activity and is able to induce sensitization after oral administration in a murine (balb/c mice) model. | 2017 | 28606183 |
| anisakis haemoglobin is a main antigen inducing strong and prolonged immunoreactions in rats. | anisakis simplex larvae are well known to cause gastrointestinal and allergic manifestations after ingestion of parasitized raw or undercooked seafood. the antibody recognition dynamics against the components of anisakis larval antigen after primary and re-infection with anisakis live larvae remain unclear. for this study, immunoblot analyses of serum igg, ige, and igm against anisakis larval somatic extract were performed in rats that had been orally inoculated with a. simplex live larvae. mult ... | 2017 | 28497226 |
| a case of small intestinal intussusception caused by anisakiasis diagnosed and treated by upper gastrointestinal endoscopy. | a 35-year-old man attended our hospital with complaining of epigastric pain and vomiting. he was diagnosed with bowel intussusception based on the target sign revealed in the upper jejunum by abdominal computed tomography. however, the cause of the intussusception was not clear. insertion of an endoscope into the jejunum revealed prominent edema in the upper part of the jejunum, and anisakis simplex was identified at the site and removed. symptoms rapidly improved after endoscopic treatment. we ... | 2017 | 28781357 |
| excretory/secretory products of anisakid nematodes: biological and pathological roles. | parasites from the family anisakidae are widely distributed in marine fish populations worldwide and mainly nematodes of the three genera anisakis, pseudoterranova and contracaecum have attracted attention due to their pathogenicity in humans. their life cycles include invertebrates and fish as intermediate or transport hosts and mammals or birds as final hosts. human consumption of raw or underprocessed seafood containing third stage larvae of anisakid parasites may elicit a gastrointestinal di ... | 2017 | 28645306 |
| atrial fibrillation in anaphylaxis. | the relationship between anaphylaxis and cardiovascular events has been reported in the past. while skin and respiratory symptoms are usually the most common and the first to appear, cardiovascular complications play a key role and represent the leading cause of death in anaphylaxis. | 2017 | 28601540 |
| anisakis sensitivity in italian children: a prospective study. | 2017 | 28398206 | |
| structural characterization of as-mif and hjab1 during the inhibition of cell-cycle regulation. | the biological activities of macrophage migration inhibitory factor (mif) might be mediated through a classical receptor-mediated or non-classical endocytic pathway. jab1 (c-jun activation domain-binding protein-1) promotes the degradation of the tumor suppressor, p53, and the cyclin-dependent kinase inhibitor, p27. when mif and jab1 are bound to each other in various intracellular sites, mif inhibits the positive regulatory effects of jab1 on the activity of ap-1. the intestinal parasite, anisa ... | 2017 | 28366190 |
| molecular diagnosis of allergy to anisakis simplex and gymnorhynchus gigas fish parasites. | there has been an increase in the prevalence of hypersensitivity to anisakis simplex. there are fish parasites other than anisakis simplex whose allergenicity has not yet been studied. | 2017 | 28341528 |
| ige, igg1 and igg4 response to specific allergens in sensitized subjects showing different clinical reactivity to anisakis simplex. | background. anisakis simplex hypersensitive subjects may be sensitized without clinical allergy, or experience acute symptoms or chronic urticaria induced by raw fish. we studied whether the 3 subgroups differ in ige, igg1 or igg4 reactivity to specific anisakis simplex allergens. methods. 28 anisakis simplex-hypersensitive adults, 11 with acute symptoms, 9 with chronic urticaria, and 8 sensitized were studied. ige, igg1 and igg4 to rani s 1, 5, 9 and 10 were sought by elisa. ige and igg4 to nan ... | 2017 | 28294584 |
| determination of anti-anisakis simplex antibodies and relationship with αβ and γδ lymphocyte subpopulations in patients with crohn's disease. | the etiology of crohn's disease (cd) is still unknown although new theories are based on defects in innate immunity. we have previously shown a decrease in γδ t cells in cd patients. previous studies have shown a high prevalence of anti-a. simplex immunoglobulins in cd patients. the diminution of γδ t cells in the peripheral blood and intestinal mucosa of cd patients may create a state of immunosuppression that would facilitate a. simplex infection. | 2017 | 28168577 |
| population parameters and mito-nuclear mosaicism of anisakis spp. in the adriatic sea. | recombinant genotypes of a. simplex sensu stricto (s.s.) and a. pegreffii, two species of anisakis simplex complex found in sympatric waters of the mediterranean sea, are believed to be a product of interspecific hybridisation and/or dna introgression. in contrast, such events within an allopatric area as the adriatic sea are unlikely to occur and therefore observed recombination should be assessed more closely. we have genotyped 525 anisakids collected from migratory and non-migratory fish of t ... | 2017 | 28126544 |
| contracaecum osculatum and other anisakid nematodes in grey seals and cod in the baltic sea: molecular and ecological links. | populations of grey seals (halichoerus grypus), sprats (sprattus sprattus) and cod (gadus morhua) in the baltic sea are relatively stationary. the present work, applying classical and molecular helminthological techniques, documents that seals and cod also share a common parasite, the anisakid nematode contracaecum osculatum, which uses seals as the final host and fish as transport hosts. sequencing mitochondrial genes (cox1 and cox2) in adult worms from seals and third-stage larvae from livers ... | 2017 | 28124629 |
| occurrence of nematodes of the genus anisakis in mediterranean and atlantic fish marketed in sardinia. | anisakiasis is a gastrointestinal fish-borne zoonosis caused by the ingestion of third stage larvae of the genus anisakis. between january and december 2013, 1112 specimens of four commercial fish species (engraulis encrasicolus, merluccius merluccius, scomber colias and trachurus mediterraneus) marketed in sardinia (italy) were examined for anisakis sp. the overall prevalence of anisakis spp larvae was 39.9%, all morphologically identified as type i. scomber colias showed the highest prevalence ... | 2017 | 28299289 |
| anisakis simplex complex: ecological significance of recombinant genotypes in an allopatric area of the adriatic sea inferred by genome-derived simple sequence repeats. | the genus anisakis includes nine species which, due to close morphological resemblance even in the adult stage, have previously caused many issues in their correct identification. recently observed interspecific hybridisation in sympatric areas of two closely related species, anisakis simplex sensu stricto (s.s.) and anisakis pegreffii, has raised concerns whether a f1 hybrid generation is capable of overriding the breeding barrier, potentially giving rise to more resistant/pathogenic strains in ... | 2017 | 28057461 |
| survey on the presence of a. simplex s.s. and a. pegreffii hybrid forms in central-western mediterranean sea. | in this study, 1029 fish and cephalopod samples came from central-western mediterranean (fao 37.1.1 and fao 37.1.3) were analysed for anisakidae larvae research with the aim to identify possible hybridisations between anisakis pegreffii and anisakis simplex s.s. species. a total of 1765 larvae were detected, with prevalence values between 8.1% and 100%. the morphologic analysis revealed characters attributable to morphotype i of anisakis in 98.5% of the examined larvae, while 1.5% belonged to th ... | 2016 | 27568095 |
| anisakiasis mimics cancer recurrence: two cases of extragastrointestinal anisakiasis suspected to be recurrence of gynecological cancer on pet-ct and molecular biological investigation. | we report two cases of anisakiasis lesions that were initially suspected to be recurrence of gynecological cancer by positron emission tomography-computed tomography (pet-ct). both cases were extragastrointestinal anisakiasis that is very rare. | 2016 | 27112922 |
| no more time to stay 'single' in the detection of anisakis pegreffii, a. simplex (s. s.) and hybridization events between them: a multi-marker nuclear genotyping approach. | a multi-marker nuclear genotyping approach was performed on larval and adult specimens of anisakis spp. (n = 689) collected from fish and cetaceans in allopatric and sympatric areas of the two species anisakis pegreffii and anisakis simplex (s. s.), in order to: (1) identify specimens belonging to the parental taxa by using nuclear markers (allozymes loci) and sequence analysis of a new diagnostic nuclear dna locus (i.e. partial sequence of the ef1 α-1 ndna region) and (2) recognize hybrid categ ... | 2016 | 27046418 |
| scavenging hagfish as a transport host of anisakid nematodes. | hagfish are the most primitive craniates and scavengers, feeding on dead organisms as well as fish and invertebrates. hagfish play an important ecological role in recycling nutrients, helping to recycle biomass from the upper water column. we investigated 265 specimens of four hagfish species, including eptatretus burgeri, eptatretus yangi, eptatretus sheni and eptatretus taiwanae from northeastern taiwanese waters of the northwestern pacific from november 2013 to june 2014. eight species of ani ... | 2016 | 26872923 |
| anisakidosis: a fortuitous mimicker of gastrointestinal malignancy. | a 51-year-old woman presented with epigastric pain, vomiting and diarrhoea. her sister was recently diagnosed with duodenal adenocarcinoma, manifesting similar symptoms. imaging revealed thickened gastric antrum with enlarged local lymph nodes. endoscopy illustrated 3 worms embedded in the antral wall, identified as anisakis simplex larvae. larvae removal and a 2-week albendazole regimen treated the symptoms. with globalisation of cultural culinary practices, physicians must be vigilant of anisa ... | 2016 | 27600057 |
| species composition and infection dynamics of ascaridoid nematodes in barents sea capelin (mallotus villosus) reflecting trophic position of fish host. | capelin (mallotus villosus) is among the most abundant fish species in the barents sea, and represents a critical food source for many predators in the area including atlantic cod and harp seal. in norway, the fish is of economic importance since whole capelin and roe are valuable export products. despite its economic and ecological importance, the parasites of barents sea capelin are poorly known. however, the presence of parasites in the edible parts may adversely affect product quality and co ... | 2016 | 27473836 |
| nonoccupational airborne-induced anaphylaxis caused by anisakis simplex. | 2016 | 27326991 | |
| anisakiasis causing acute dysentery in malaysia. | human anisakiasis is a zoonosis acquired by eating raw or undercooked infected seafood. herein, we report a case of acute dysentery caused by anisakiasis in a 64-year-old man in malaysia. a colonoscopy was performed and a nematode larva was found penetrating the mucosa of the ascending colon. bleeding was observed at the site of penetration. y-shaped lateral epidermal cords were seen from the cross section of the worm, which is a prominent feature of anisakis larva. molecular analysis using poly ... | 2016 | 27325803 |
| molecular epidemiology and risk factors for anisakis simplex s.l. infection in blue whiting (micromesistius poutassou) in a confluence zone of the atlantic and mediterranean: differences between a. simplex s.s. and a. pegreffii. | our study determined parameters of parasitization of anisakis simplex s.l. in micromesistius poutassou in a confluence zone of the atlantic and mediterranean, with a total prevalence of 82%. also, in the three seasons analyzed, high prevalence's values were found, reaching 100% in spring; however mean intensity and abundance values were higher in winter. the use of molecular techniques to differentiate between anisakis genotypes of the larvae characterized allowed obtaining values of 99.7% anisa ... | 2016 | 27289194 |
| cytokine profile in patients infected with anisakis simplex in endemic areas: dietary intervention with polyphenols. a working hypothesis. | anisakiasis is a zoonosis due to infection with the nematode anisakis (a.) simplex. humans represent an accidental host after ingesting raw fish infected with a. simplex. a. simplex larvae lead to a robust host immune response mostly at gastro-intestinal level and, usually, t helper (h)2 lymphocytes play a protective against this nematode. however, larvae can elude immunity triggering activation of t regulatory (treg) cells, which, in turn, suppress th2 responses via production of interleukin (i ... | 2016 | 27150602 |
| inactivation of anisakis simplex l3 in the flesh of white spotted conger (conger myriaster) by high hydrostatic pressure and its effect on quality. | koreans consume much seafood; the country is surrounded on the east, west and south by the sea. koreans have eaten raw sashimi for a long time. however, a concern in the raw sea food industry is that the parasitic nematode anisakis simplex l3 occurs naturally in marine fish. thus, the fishery industry needs a non-thermal processing method. high hydrostatic pressure (hpp) has been demonstrated to be effective. white spotted conger flesh containing 20 live larvae was exposed to different pressures ... | 2016 | 27117731 |
| parasites as biological tags for stock discrimination of beaked redfish (sebastes mentella): parasite infra-communities vs. limited resolution of cytochrome markers. | the use of parasites as biological tags for discrimination of fish stocks has become a commonly used approach in fisheries management. metazoan parasite community analysis and anisakid nematode population genetics based on a mitochondrial cytochrome marker were applied in order to assess the usefulness of the two parasitological methods for stock discrimination of beaked redfish sebastes mentella of three fishing grounds in the north east atlantic. multivariate, model-based approaches demonstrat ... | 2016 | 27104735 |
| ani s 11-like protein is a pepsin- and heat-resistant major allergen of anisakis spp. and a valuable tool for anisakis allergy component-resolved diagnosis. | anisakis simplex is a fish parasite responsible for gastrointestinal and allergic symptoms in humans. the ani s 11-like protein has been proposed as an anisakis allergen because its primary structure is similar to that of ani s 11. the aims of this work were to analyse the frequency of detection of the ani s 11-like protein and assess its diagnostic value. | 2016 | 27049862 |
| molecular identification of anisakis and hysterothylacium larvae in commercial cephalopods from the spanish mediterranean coast. | this study aims to investigate the occurrence of nematode larvae in commercial cephalopods in the western mediterranean sea. a total of 202 animals comprising 123 broadtail shortfin squid (illex coindetii), 34 european squid (loligo vulgaris) and 45 common octopus (octopus vulgaris) were examined using enzymatic digestion. a total of 31 larvae were isolated (prevalence: 14.6%) and identified using molecular analyses which included pcr and sequencing of the its (its1-5.8s rdna-its2) region. phylo ... | 2016 | 26995720 |
| parasitation of sea trout (salmo trutta trutta l.) from the spawning ground and german coastal waters off mecklenburg-western pomerania, baltic sea. | a total of 52 sea trouts, salmo trutta trutta, were studied for parasites, originating from german freshwater streams and coastal waters of the baltic sea. while 35 specimens were caught mainly close to the shoreline in the baltic sea, 17 were sampled during their spawning migration in warnow river and other neighboring rivers in mecklenburg-western pomerania. a total of 12 different metazoan parasite species were found in sea trout originating from the baltic sea, including five digeneans, two ... | 2016 | 26374539 |
| tolerability to dogfish in children with fish allergy. | fish is a potent food allergen. the aim of this work is to demonstrate that dogfish, a small shark, has low allergenicity in both its clinical tolerance as well as its molecular structure. | 2016 | 26337570 |
| first record of anisakis simplex third-stage larvae (nematoda, anisakidae) in european hake merluccius merluccius lessepsianus in egyptian water. | the prevalence of infection and the identification of anisakid larvae in european hake merluccius merluccius lessepsianus from hurghada city, red sea governorate, egypt, were investigated. fish samples were collected during the period of february and november 2014. twenty-two (36.66%) out of sixty examined fish specimens were found to be naturally infected with anisakis type i larvae mostly found as encapsulated larvae in visceral organs. there was a positive relationship between host length/wei ... | 2016 | 26925257 |
| high prevalence of anisakis simplex hypersensitivity and allergy in sicily, italy. | anisakis simplex can elicit allergic reactions when ingested in raw or marinated fish. the prevalence of a simplex hypersensitivity and allergy in sicily (italy), an area where the consumption of raw or marinated fish is very common, has not been investigated thus far. | 2016 | 26815707 |
| development of liquid chromatography-tandem mass spectrometry methods for the quantitation of anisakis simplex proteins in fish. | the parasite anisakis simplex is present in many marine fish species that are directly used as food or in processed products. the anisakid larvae infect mostly the gut and inner organs of fish but have also been shown to penetrate into the fillet. thus, human health can be at risk, either by contracting anisakiasis through the consumption of raw or under-cooked fish, or by sensitisation to anisakid proteins in processed food. a number of different methods for the detection of a. simplex in fish ... | 2016 | 26787163 |
| chronic spontaneous urticaria and internal parasites--a systematic review. | chronic spontaneous urticaria (csu) is defined as persistent wheals, angioedema, or both lasting for >6 weeks due to known or unknown causes. some epidemiological studies and case reports suggest that internal parasite infections (pi) can cause csu. here, we provide a systematic overview of published findings on the prevalence and relevance of pi in csu and we discuss possible pathomechanisms. the prevalence of pi in csu was investigated by 39 independent studies and comorbidity reportedly range ... | 2016 | 26648083 |
| molecular cloning and expression of a new major allergen, ani s 14, from anisakis simplex. | the nematode anisakis simplex is a representative parasite infecting marine animals. when third stage larvae of a. simplex infecting fish and squids are ingested by humans, individuals previously sensitized by this parasite may experience ige-mediated allergic reactions. so far, as many as 13 kinds of proteins (ani s 1-13) have been identified as a. simplex allergens but several more unknown allergens are suggested to exist. in this study, therefore, chemiluminescent immunoscreening of an expres ... | 2015 | 26537648 |
| fish-allergic patients may be able to eat fish. | reported fish allergy prevalence varies widely, with an estimated prevalence of 0.2% in the general population. sensitization to fish can occur by ingestion, skin contact or inhalation. the manifestations can be ige or non-ige mediated. several fish allergens have been identified, with parvalbumins being the major allergen in various species. allergenicity varies among fish species and is affected by processing or preparation methods. adverse reactions after eating fish are often claimed to be ' ... | 2015 | 25666551 |
| expression of genes encoding the enzymes for glycogen and trehalose metabolism in l3 and l4 larvae of anisakis simplex. | trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including anisakis simplex. nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. the aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen-trehalose-6-phosphate synthase (tps), trehalose-6-phosphate phosphatase (tpp), glycogen synthase (gs), and glycogen ph ... | 2015 | 26783451 |
| natural products: perspectives in the pharmacological treatment of gastrointestinal anisakiasis. | to evaluate the activity of different natural products against l3 larvae of anisakis type i. | 2015 | 26321513 |
| nematodes parasitizing trachurus trachurus (l.) and boops boops (l.) from algeria. | a total of 455 boops boops (linnaeus, 1758) and 953 trachurus trachurus linnaeus, 1758 from the east coast of algeria were examined for their parasitic nematoda. two hundred ninety-five specimens of larval stages l3 and l4 were collected from the peritoneal cavity of these two examined fishes. photonic and scanning electronic microscopy (sem) studies were performed on these larvae specimens in order to characterize their morphology. two different species of nematoda (anisikidae) were identified: ... | 2015 | 26220559 |
| aniserp: a new serpin from the parasite anisakis simplex. | serine proteinase inhibitors (serpins) finely regulate serine proteinase activity via a suicide substrate-like inhibitory mechanism. in parasitic nematodes, some serpins interact with host physiological processes; however, little is known about these essential molecules in anisakis. this article reports the gene sequencing, cloning, expression and preliminary biochemical and bioinformatically-based structural characterization of a new anisakis serpin (aniserp). | 2015 | 26215984 |
| foods, drugs and environmental factors: novel kounis syndrome offenders. | kounis syndrome is hypersensitivity coronary disorder induced by various types of environmental exposures, drugs, conditions and stents. allergic, hypersensitivity, anaphylactic and anaphylactoid reactions are associated with this syndrome. the disorder manifests as coronary spasms, acute myocardial infarction and stent thrombosis and affects the cerebral and mesenteric as well as coronary arteries. importantly, its manifestations are broad and its etiology is continuously increasing. recently, ... | 2015 | 26134186 |
| anisakidae in beluga whales delphinapterus leucas from hudson bay and hudson strait. | a total of 190 nematodes was isolated from the stomachs of 13 beluga whales delphinapterus leucas from the arctic part of hudson bay and hudson strait. infection intensity ranged from 1 to 57 specimens and prevalence was 84.62%. morphological examination of the nematodes revealed the presence of 3 species: pseudoterranova decipiens sensu lato, contracaecum osculatum s.l., and anisakis simplex s.l. molecular analysis by pcr-based restriction fragment length polymorphism (pcr-rflp) resulted in the ... | 2015 | 26119295 |
| the complete mitochondrial genome of pseudoterranova azarasi and comparative analysis with other anisakid nematodes. | anisakiasis/anisakidosis caused by anisakid nematodes is an emerging infectious disease that can cause a wide range of clinical syndromes and are difficult to diagnose and treat in humans. in spite of their significance as pathogens, the systematics, genetics, epidemiology and biology of these parasites remain poorly understood. in the present study, we sequenced the complete mitochondrial (mt) genome of pseudoterranova azarasi, which is one of the most important zoonotic anisakid parasites. the ... | 2015 | 25998795 |
| cytokine signature and antibody-mediated response against fresh and attenuated anisakis simplex (l3) administration into wistar rats: implication for anti-allergic reaction. | the third larval stage (l3) of anisakis simplex (anisakidae) is one of the zoonotic parasitic nematodes in the musculature and visceral organs of marine fishes belonging to family moronidae. the consumption of these high-commercial-value fish is widespread in many countries around the mediterranean sea including egypt. the presence of these larvae in fish muscles poses a potential consumer hazard due to the parasite's ability to cause anisakidosis. forty-two out of 60 (70%) of the european seaba ... | 2015 | 25982570 |
| a case of human infection with anisakis simplex in taiwan. | 2015 | 25963619 | |
| antihelmintic effects of nutmeg (myristica fragans) on anisakis simplex l3 larvae obtained from micromesistius potassou. | anisakis simplex is a foodborne pathogen that can produce human infections and allergic reactions due to the high consumption of raw fish. the seeds of myristica fragans (myristicaceae), popularly known as nutmeg, are worldwide used as a culinary spice due to its flavour and properties in food preservation. a nutmeg extract was prepared, analyzed, screened for cytotoxicity and tested against anisakis simplex l3 larvae. in order to detect the biologically active constituents of the extract, myris ... | 2015 | 25890576 |
| petromyzon marinus (petromyzontidae), an unusual host for helminth parasites in western europe. | the sea lamprey petromyzon marinus, which is among the most phylogenetically ancient vertebrates, is a hematophagous ectoparasite that feeds on vertebrates and is considered vulnerable in europe but is a pest in the north american great lakes. we conducted a literature review of helminth parasites of p. marinus and investigated postmetamorphic lampreys sampled in rivers and northeast atlantic coastal waters (western france) during spawning migration. based on the literature review, 16 helminth t ... | 2015 | 25850404 |
| prevalence of anisakid nematode larvae infecting some marine fishes from the libyan coast. | this study examined eight hundred ninety six marine fishes belonging to nine different fish species; synodus saurus; merluccius merluccius; trachurus mediterraneus; serranus cabrilla; mullus surmuletus; diplodus annularis; spicara maena; siganus rirulatus and liza ramada. the fishes were bought from fish markets at five different sites on libyan coast, from january to december 2013, for study the anisakids larvae among them. the results showed that 344/896 fishes (38.4%) were infected with anisa ... | 2015 | 26939239 |
| antigenicity of anisakis simplex s.s. l3 in parasitized fish after heating conditions used in the canning processing. | some technological and food processing treatments applied to parasitized fish kill the anisakis larvae and prevent infection and sensitization of consumers. however, residual allergenic activity of parasite allergens has been shown. the aim here was to study the effect of different heat treatments used in the fish canning processing industry on the antigen recognition of anisakis l3. bigeye tuna (thunnus obesus) and yellowfin tuna (thunnus albacares) were experimentally infected with live l3 ani ... | 2015 | 24888867 |
| tlr2-dependent amelioration of allergic airway inflammation by parasitic nematode type ii mif in mice. | in our previous studies, the recombinant type ii macrophage migration inhibitory factor homologue (ras-mif) secreted from anisakis simplex suppressed experimental inflammation mouse model through il-10 production and cd4(+)cd25(+)foxp3(+) t-cell recruitment. also, tlr2 gene expression was significantly increased following ras-mif treatment. to know the relation between tlr2 and amelioration mechanisms of ras-mif, we induced allergic airway inflammation by ovalbumin and alum with or without ras-m ... | 2015 | 25559209 |
| anisakid nematodes in beaked redfish (sebastes mentella) from three fishing grounds in the north atlantic, with special notes on distribution in the fish musculature. | parasitic anisakid nematodes commonly occur in the musculature and visceral organs of many fish species from the north atlantic. in this respect, the presence of the third stage larvae of anisakis spp. in the fish musculature may pose a potential consumer hazard due to the parasite's ability to cause anisakidosis. thus, knowledge on the occurrence and distribution of these potentially zoonotic parasites in the commercially important north atlantic fish species is crucial in order to evaluate and ... | 2015 | 25498327 |
| relationships between t cell and ige/igg4 epitopes of the anisakis simplex major allergen ani s 1. | anisakiasis is a global disease caused by the consumption of raw or lightly cooked fish parasitized with third-stage anisakis larvae. anisakis simplex allergens may cause severe allergic reactions including angio-oedema, urticaria and anaphylaxis. approximately 80% of allergic patients have allergen-specific ige against ani s 1, and the diagnostic value of testing for antibodies to ani s 1 has been extensively demonstrated. however, no previous studies have investigated the molecular aspects of ... | 2015 | 25495594 |
| removal of anisakis simplex allergens from infected fish during the washing step of surimi production. | the washing operation of fish muscle is one of the key steps in the production of surimi. the aim of this study was to assess in parasitised minced fish the effect of the washing steps on the allergen removal of anisakis simplex and on protein yield during surimi processing. experimentally infected hake (merluccius merluccius) (50 anisakis simplex s.s l3 larvae per 100 g of muscle) underwent three successive washing steps with water, phosphate buffer (20 mmol l(-1) ), sodium bicarbonate (60 mmol ... | 2015 | 25378259 |
| [role of helminth antigens in the abnormal mitosis of bone marrow cells in laboratory animals]. | the intraabdominal administration of somatic extracts of the cestodes hydatigera taeniaformis batsch 1786, lamarck, 1816 and diphyllobothrium latum linnaeus, 1758 and the nematodes anisakis simplex larva rudolphi 1809, toxocara canis railliet et henry, 1912 in albino mice proved that these helminths had a karyopathic effect on the bone marrow cells of the animals. the antigenic composition of these extracts was investigated using the agar gel immunodiffusion test. the antigenic composition of th ... | 2015 | 25924282 |
| positive associations between infections of toxoplasma gondii and seropositivity with anisakis simplex in human patients suffering from chronic urticaria. | toxoplasma gondii is a food-borne and orofecal microorganism which produces chronic infection, and attempts have been made to prove its negative association with atopy in the context of the hygiene hypothesis. anisakis simplex is a fish parasite associated with chronic urticaria (cu) in endemic regions. we analysed the relationship between both infectious agents in cu. we included 42 patients with chronic urticaria (18 patients with cu associated with a. simplex sensitization and 24 not sensitiz ... | 2015 | 24991841 |
| occurrence and prevalence of fish-borne anisakis larvae in the spotted mackerel scomber australasicus from taiwanese waters. | anisakid nematodes have been found in a variety of marine fishes throughout the world and they are known to cause anisakiasis in human hosts. the present study investigated the prevalence of potentially zoonotic anisakid larvae in spotted mackerel caught from taiwanese waters where fish represents an important food sources. anisakis third-stage larvae (l3, n=502) were isolated from 250 spotted mackerel scomber australasicus. anisakis l3 larvae were divided morphologically into two types, anisaki ... | 2015 | 25733490 |
| serine proteases of parasitic helminths. | serine proteases form one of the most important families of enzymes and perform significant functions in a broad range of biological processes, such as intra- and extracellular protein metabolism, digestion, blood coagulation, regulation of development, and fertilization. a number of serine proteases have been identified in parasitic helminths that have putative roles in parasite development and nutrition, host tissues and cell invasion, anticoagulation, and immune evasion. in this review, we de ... | 2015 | 25748703 |
| anisakis pegreffii larvae in sea eels (astroconger myriaster) from the south sea, republic of korea. | anisakis simplex sensu stricto (s.s.), anisakis pegreffii, anisakis berlandi (=a. simplex sp. c), and anisakis typica are the 4 major species of anisakis type i larvae. in the republic of korea (korea), a. pegreffii, a. berlandi, and a. typica larvae in fish hosts has seldom been documented. in this study, molecular analysis was performed on anisakis larvae from the sea eels (astroconger myriaster), the major source of human anisakiasis in korea, collected from tongyeong city, a southern coastal ... | 2015 | 26174831 |
| macroparasites of allis shad (alosa alosa) and twaite shad (alosa fallax) of the western iberian peninsula rivers: ecological, phylogenetic and zoonotic insights. | samples of anadromous alosa alosa (clupeidae) (n = 163) and alosa fallax (clupeidae) (n = 223), caught in western iberian peninsula rivers from 2008 to 2013, were examined for buccal, branchial and internal macroparasites, which were identified using morphological and molecular methods. alosa alosa were infected with anisakis simplex s.s., anisakis pegreffii, hysterothylacium aduncum, rhadinorhynchus pristis, mazocraes alosae, hemiurus appendiculatus, ceratothoa italica and an unidentified ergas ... | 2015 | 26141433 |
| anisakis infection in allis shad, alosa alosa (linnaeus, 1758), and twaite shad, alosa fallax (lacépède, 1803), from western iberian peninsula rivers: zoonotic and ecological implications. | spawning individuals of allis shad, alosa alosa (linnaeus, 1758), and twaite shad, alosa fallax (lacépède, 1803), were sampled from three rivers on the atlantic coast of the iberian peninsula (ulla, minho, mondego) during 2008 to 2013 to assess the presence of the zoonotic marine parasite anisakis spp. larvae. the results revealed that both shad species were infected by third-larval stage anisakis simplex s.s. and anisakis pegreffii. the latter is reported in mixed infections in both shad specie ... | 2015 | 25810220 |
| fishing area and fish size as risk factors of anisakis infection in sardines (sardina pilchardus) from iberian waters, southwestern europe. | the sardine (sardina pilchardus) is a fish commonly consumed and appreciated in many countries, although they are more likely to be eaten fresh in western mediterranean countries such as spain, portugal, france or italy. a molecular epidemiological survey of sardines from 5 fishing areas of the spanish mediterranean (málaga, southern spain) and atlantic coasts (southern: cádiz and isla cristina; northern: a coruña and ondarroa) was carried out to determine the presence of anisakis spp. larvae. t ... | 2015 | 25770431 |
| pathogenic potential of two sibling species, anisakis simplex (s.s.) and anisakis pegreffii (nematoda: anisakidae): in vitro and in vivo studies. | the pathogenic potentials of two sibling nematodes anisakis simplex sensu stricto (s.s.) and a. pegreffii were compared by in vitro and in vivo studies. live third-stage larvae of each species were subjected to agar blocks made using pbs or rpmi-1640, overlaid with different supernatants (artificial gastric juice, pbs, and rpmi-1640), and their penetration ability was compared. their tolerance of artificial gastric juice was also tested. further, they were introduced into rats by gastric intubat ... | 2015 | 25685821 |
| haemoglobin, a new major allergen of anisakis simplex. | gastro-allergic anisakiasis and anisakis sensitisation associated chronic urticaria are diseases which differ in their ige and igg4 responses against both crude extract and specific allergens. anisakis and ascaris are closely related nematodes that usually cause problems with specificity in immunodiagnostics. in this study we measured ige and igg4 antibodies against anisakis simplex sensu lato (s. l.) and ascaris suum haemoglobins in sera of 21 gastro-allergic anisakiasis and 23 chronic urticari ... | 2015 | 25683373 |
| molecular diagnosis of cause of anisakiasis in humans, south korea. | anisakiasis in humans in south korea has been considered to be caused exclusively by the larvae of anisakis simplex sensu stricto and pseudoterranova decipiens. recently, however, dna sequencing of larvae from 15 of 16 anisakiasis patients confirmed the cause to be anisakis pegreffii infection. molecular analysis should be performed for all extracted larvae. | 2015 | 25625427 |
| molecular identification of anisakis and hysterothylacium larvae in marine fishes from the east china sea and the pacific coast of central japan. | anisakiasis is a human disease caused by the accidental ingestion of larvae belonging to the family anisakidae. three fish species, the small yellow croaker pseudosciaena polyactis, the mackerel pneumatophorus japonicus and the hairtail trichiurus haumela are important source for food products in the east china sea. the prevalence and the identification of anisakidae larvae in these fishes will benefit the prevention and control of anisakiasis. in this study, fish samples were obtained from fish ... | 2015 | 25617845 |
| genetic identification and distribution of the parasitic larvae of anisakis pegreffii and anisakis simplex (s. s.) in european hake merluccius merluccius from the tyrrhenian sea and spanish atlantic coast: implications for food safety. | the consumption of the hake merluccius merluccius is widespread in european countries, where this fish has a high commercial value. to date, different larval species of anisakis have been identified as parasites in m. merluccius from european waters, anisakis pegreffii and anisakis simplex (s. s.) being the two most common. the aim of the study is to present data on the occurrence of anisakis spp. larvae in the viscera and flesh of m. merluccius. consequently, the distribution and infection rate ... | 2015 | 25584776 |
| three anisakis spp. isolated from toothed whales stranded along the eastern adriatic sea coast. | knowledge concerning cetacean ecology in the mediterranean is limited but important for sustainable planning and enforcement of appropriate conservation measures. any information that might help to elucidate their ecology is essential. we explored the population and genetic structures of anisakis spp. nematodes isolated from four toothed whale species - bottlenose dolphins (tursiops truncatus), striped dolphins (stenella coeruleoalba), risso's dolphins (grampus griseus) and cuvier's beaked whale ... | 2015 | 25449948 |
| occurrence of anisakid nematode larvae in chub mackerel (scomber japonicus) caught off korea. | chub mackerel (scomber japonicus) is a pelagic fish species widely distributing in the indo-pacific and a commercially important fish species in korea. it is known to harbor anisakid nematodes larvae, and ingesting the raw or undercooked fish can accidentally cause human infection. in this study, we isolated the nematode larvae in 417 chub mackerel caught from 7 sampling locations around the korean peninsula in 2011 and 2012, and identified them by pcr-rflp of the its (internal transcribed space ... | 2014 | 25268324 |
| anisakidae in fishing products sold in sicily. | one of the parasite diseases associated with the consumption of raw fish that occurs with some frequency is the anisakiasis, a human disease caused by the accidental ingestion of larval nematodes of the genus anisakis, family anisakidae. at the national reference centre for anisakiasis (c.re.n.a.) from october 2012 to february 2013, a number of 231 bony fish (trichiuridae, engraulidae, scombridae and clupeidae) were received from different fishing sites in sicily. anisakis pegreffii is the main ... | 2014 | 27800344 |
| molecular identification of anisakis species (nematoda: anisakidae) from marine fishes collected in turkish waters. | anisakid nematodes are important etiological agents for zoonotic human anisakiasis (or anisakidosis). these parasites in the turkish waters still remain unexplored. this study aims the molecular identification of anisakis species in turkey's coast from black, aegean and mediterranean sea and specifically to screen for zoonotic species in commonly commercialized a total of 1145 fish belonging to 31 different species using both polymerase chain reaction-restriction fragment length polymorphism ana ... | 2014 | 24485564 |
| ecology and genetic structure of zoonotic anisakis spp. from adriatic commercial fish species. | consumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of live anisakis larvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. we investigated the population dynamics of anisakis spp. in commercially important fish-anchovies (anisakis), sardines (sardina pilchardus), european hake (merluccius merluccius), whiting (merlangius merlangus), chub mackerel (scomber japonicus), and atlantic bl ... | 2014 | 24317085 |
| experimental demonstration of pathogenic potential of anisakis physeteris and anisakis paggiae in wistar rats. | anisakis morphotype i is the principal etiologic agent of human anisakiasis, with differences in pathogenicity found between the anisakis simplex s.s. and a. pegreffii species; however, the role of morphotype ii larvae in this illness is not well understood. the purpose of this study is to verify the ability of morphotype ii larvae to invade tissues via the experimental infection of wistar rats, an animal model which simulates infection in humans. in the in vivo assay, 7.1% (4/56 l3 morphotype i ... | 2014 | 25240961 |
| activity of tea tree (melaleuca alternifolia) essential oil against l3 larvae of anisakis simplex. | nematicidal activity of melaleuca alternifolia essential oil, commonly known as tea tree oil (tto), was assayed in vitro against l3 larvae of anisakis simplex. the results showed a mortality of 100% for concentrations between 7 and 10 μl/ml after 48 h of incubation, obtaining an ld50 value of 4.53 μl/ml after 24 hours and 4.27 μl/ml after 48 hours. concentration-dependent inhibition of acetylcholinesterase was observed for tea tree essential oil showing inhibition values of 100% at 100 μl/ml. th ... | 2014 | 24967378 |
| occurrence of anisakis simplex sensu stricto in imported atlantic mackerel (scomber scombrus) represents a risk for turkish consumers. | anisakid larvae are a prevalent food-borne pathogen that has been found in numerous fish species destined for human consumption. the accidental consumption of infected raw or poorly cooked fish may cause gastroenteric diseases and allergies in humans. in spite of the fact that thorough cooking or freezing kills anisakis worms, this method does not destroy their allergenic capacity. the presence of a. simplex (s.s.) in seafood products may present a health risk for consumers. in turkey, atlantic ... | 2014 | 24935687 |
| anisakis/ascaris ige ratio improves specificity for the diagnosis of anisakis simplex sensitization in travellers and immigrants. | anisakis simplex is a fish parasite responsible for human infection and is able to induce ige-mediated reactions with several clinical manifestations. laboratory diagnosis of anisakis allergy is based on the detection of specific ige using parasite whole antigen. unfortunately, these diagnostic tools detect cross-reactivities with other nematodes and micro-organisms leading to low specificity of the diagnostic tests. the aim of this retrospective study was to assess the diagnostic value of speci ... | 2014 | 24911935 |
| presence of anisakis simplex (rudolphi, 1809 det. krabbe, 1878) and hysterothylacium aduncum (rudolphi, 1802) (nematoda; anisakidae) in runts of farmed atlantic salmon, salmo salar l. | one hundred farmed atlantic salmon, salmo salar l., were examined for the presence of nematodes by digestion of tissue in hcl-pepsin solution. all fish were sampled from one cage in a fish farm on the norwegian south-west coast. fifty harvest quality salmon, that is, salmon for human consumption (mean 5.4 kg, variation 3.0-7.6 kg), were sampled at the processing line while 50 salmon runts (mean 1.1 kg, variation 0.4-1.8 kg), discarded due to poor performance, were sampled from the discard bin af ... | 2014 | 23488892 |
| allergenicity of two anisakis simplex allergens evaluated in vivo using an experimental mouse model. | anisakis (anisakidae) is one of the most important causes of helminth-induced allergic reactions and elicits clinical responses that include urticaria, rhinitis, bronco-constriction, cough, and/or gastrointestinal symptoms. more than 13 reactive allergens have been identified in the serum of anisakis allergy patients, but the allergenicity of only a few of these have been evaluated in vivo using a mouse model. to evaluate the allergenicity of two important allergens, ani s 1 and ani s 9, we indu ... | 2014 | 25300761 |
| putative hybrids between two anisakis cryptic species: molecular genotyping using high resolution melting. | the genus anisakis includes nine recognized species and the complex of cryptic species anisakis simplex s. l. is often associated with the human disease known as anisakiasis. during the last decades the use of nuclear ribosomal its allowed the identification and description of numerous anisakid nematodes and the discovery of recombinant genotypes or putative hybrids even in other parasitic helminths, such as those between a. simplex sensu stricto and a. pegreffii. the existence of pure hybrids o ... | 2014 | 25241034 |
| occurrence of zoonotic nematodes pseudoterranova decipiens, contracaecum osculatum and anisakis simplex in cod (gadus morhua) from the baltic sea. | baltic cod gadus morhua (a total of total 224 specimens) captured east of the island of bornholm in the southern baltic sea were subjected to a parasitological investigation between march 2013 and april 2014. full artificial digestion of fillets from 188 cod and additional investigation of livers from 36 cod were performed. cod or seal worm pseudoterranova decipiens was recorded in musculature (prevalences up to 55% and intensities up to 56 worms per fish) associated with a negative correlation ... | 2014 | 25224792 |
| [anisakis simplex larvae: infection status in marine fishes for sale in shantou]. | to investigate the infection status of anisakis simplex larvae in marine fishes for sale in shantou. | 2014 | 25223058 |
| anisakids (nematoda: anisakidae) from stomachs of northern fur seals (callorhinus ursinus) on st. paul island, alaska: parasitological and pathological analysis. | anisakid nematodes parasitize the alimentary tracts of aquatic vertebrates, including northern fur seals (callorhinus ursinus) (nfs). the main purpose of this study was to estimate the prevalence, intensity, and species composition of anisakids in nfss on st. paul island, alaska, and to analyze changes in nfs infection with these nematodes during the last decades. the study was carried out on st. paul island, alaska, in july-august 2011-2013. stomachs of 443 humanely harvested nfs males were col ... | 2014 | 25216781 |
| metazoan parasites from herring (clupea harengus l.) as biological indicators in the baltic sea. | zoographical distribution of metazoan fish parasites in herring, clupea harengus, from the baltic sea was analysed in order to use them as potential biological indicators. a total of 210 herring from six different sampling sites were investigated, harbouring 12 different parasite species [five digeneans (d), one cestode (c), three nematodes (n) and three acanthocephalans (a)]. the distribution of the parasite species differed according to region, with a distinct gradient of decreasing species ri ... | 2014 | 25119368 |
| proteomic profiling and characterization of differential allergens in the nematodes anisakis simplex sensu stricto and a. pegreffii. | the parasite species complex anisakis simplex sensu lato (anisakis simplex sensu stricto; (a. simplex s.s.), a. pegreffii, a. simplex c) is the main cause of severe anisakiasis (allergy) worldwide and is now an important health matter. in this study, the relationship of this anisakis species complex and their allergenic capacities is assessed by studying the differences between the two most frequent species (a. simplex s.s., a. pegreffii) and their hybrid haplotype by studying active l3 larvae p ... | 2014 | 24723494 |
| acetylcholinesterase secreted by anisakis simplex larvae (nematoda: anisakidae) parasitizing herring, clupea harengus: an inverse relationship of enzyme activity in the host-parasite system. | acetylcholinesterase (ache) is a key enzyme involved in nerve impulse transmission in both vertebrates and invertebrates. in addition to neuromuscular ache, many parasitic nematodes synthesize ache in secretory glands and release the enzyme into their external environment. in this study, we evaluate the activities of both somatic and secreted ache from larvae (l3) of the parasitic nematode anisakis simplex, and compare these to the ache activity in its host, herring, clupea harengus. a. simplex ... | 2014 | 24705520 |
| identification of autoclave-resistant anisakis simplex allergens. | anisakis simplex is a fish parasite able to induce allergic reactions in humans infected when eating raw or undercooked fish parasitized with viable third-stage larvae. some authors claim that exposure to nonviable anisakis material can result in allergic symptoms in previously sensitized patients, indicating that parasite allergens are resistant to the thermal treatments of usual cooking procedures. furthermore, some patients report symptoms after eating canned fish. the aim of this work was th ... | 2014 | 24680072 |
| relationships between ige/igg4 epitopes, structure and function in anisakis simplex ani s 5, a member of the sxp/ral-2 protein family. | anisakiasis is a re-emerging global disease caused by consumption of raw or lightly cooked fish contaminated with l3 anisakis larvae. this zoonotic disease is characterized by severe gastrointestinal and/or allergic symptoms which may misdiagnosed as appendicitis, gastric ulcer or other food allergies. the anisakis allergen ani s 5 is a protein belonging to the sxp/ral-2 family; it is detected exclusively in nematodes. previous studies showed that sxp/ral-2 proteins are active antigens; however, ... | 2014 | 24603892 |
| helminth allergens, parasite-specific ige, and its protective role in human immunity. | the th2 immune response, culminating in eosinophilia and ige production, is not only characteristic of allergy but also of infection by parasitic worms (helminths). anti-parasite ige has been associated with immunity against a range of helminth infections and many believe that ige and its receptors evolved to help counter metazoan parasites. allergens (ige-antigens) are present in only a small minority of protein families and known ige targets in helminths belong to these same families (e.g., ef ... | 2014 | 24592267 |
| anthelmintic activities of aporphine from nelumbo nucifera gaertn. cv. rosa-plena against hymenolepis nana. | nelumbo nucifera gaertn. cv. rosa-plena (nelumbonaceae), commonly known as lotus, is a perennial aquatic plant grown and consumed throughout asia. all parts of n. nucifera have been used for various medicinal purposes in oriental medicine. from the leaves of nelumbo nucifera gaertn. cv. rosa-plena (an aquatic plant), liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-n-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), ... | 2014 | 24583851 |
| oral inoculation of live or dead third-stage larvae of anisakis simplex in rats suggests that only live larvae induce production of antibody specific to a. simplex. | live anisakis simplex third-stage larvae (l3) penetrate gastrointestinal mucosa after they are ingested in raw or undercooked seafood, thereafter causing gastrointestinal manifestations and allergic manifestations such as urticaria and anaphylaxis. these allergic reactions are mediated by specific ige to l3 allergens, especially excretory-secretory (es) allergens. recent evidences suggest that only live larvae can cause allergic reactions, although cases attributable to ingestion of cooked, froz ... | 2014 | 24570066 |
| anisakids of seals found on the southern coast of baltic sea. | in the present study 5 grey seals (halichoerus grypus), 3 common seals (phoca vitulina) and 1 ringed seal (pusa hispida) bycaught or stranded on the polish baltic sea coast in years 2000-2006 were investigated for the infestation of parasitic anisakid nematodes. 749 of anisakids were found. the most common were: contracaecum osculatum (59.3%) and pseudoterranova decipiens (31.0%). there were also small numbers of anisakis simplex (0.8%). after performing rflp three sibling species were found. c. ... | 2014 | 24570064 |
| cross-reactivity between anisakis spp. and wasp venom allergens. | anisakiasis is caused by the consumption of raw or undercooked fish or cephalopods parasitized by live l3 larvae of nematode anisakis spp. larvae anchor to stomach mucosa releasing excretion/secretion products which contain the main allergens. it has been described that nematode larvae release venom allergen-like proteins among their excretion/secretion products. we investigated potential cross-reactivity between anisakis and wasp venom allergens. | 2014 | 24525615 |