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prevalence of helminth and coccidian parasites in swedish outdoor cats and the first report of aelurostrongylus abstrusus in sweden: a coprological investigation.this study was performed in order to gather recent epidemiological data on feline endoparasites in swedish cats. faecal samples from 205 outdoor cats were collected by their owners and submitted to the national veterinary institute for analysis. the study population was comprised of cats with access to an outdoor environment and with no history of anthelmintic treatment within the last 3 months. intestinal parasites were detected with a centrifugal flotation technique and baermann larval sedimen ...201728330485
detection of specific antibodies in cats infected with the lung nematode aelurostrongylus abstrusus.feline aelurostrongylosis, caused by the metastrongylid nematode aelurostrongylus abstrusus, is an underestimated respiratory parasitosis. its diagnosis currently mainly relies on the isolation of first stage larvae from fresh faecal samples. the aim of our study was to develop a serological test for the detection of specific antibodies against a. abstrusus by elisa. we used recombinant major sperm protein (msp) of the bovine lungworm dictyocaulus viviparus as detection antigen and evaluated two ...201728215872
occurrence and clinical significance of aelurostrongylus abstrusus and other endoparasites in danish cats.feline endoparasites are highly prevalent worldwide and may cause a variety of clinical signs in infected cats. prevalence rates are dynamic and there is limited knowledge of the current prevalence in denmark and the clinical manifestation and significance of especially the lungworm aelurostrongylus abstrusus. this study investigated the total and local prevalence of aelurostrongylus abstrusus and other endoparasites in danish cats. the clinical significance of feline aelurostrongylosis was also ...201728115180
intestinal parasites and vector-borne pathogens in stray and free-roaming cats living in continental and insular greece.this survey investigated the distribution of various intestinal parasites and vector-borne pathogens in stray and free-roaming cats living in four regions of greece. a total number of one hundred and fifty cats living in three islands (crete, mykonos and skopelos) and in athens municipality was established as a realistic aim to be accomplished in the study areas. all cats were examined with different microscopic, serological and molecular assays aiming at evaluating the occurrence of intestinal ...201728141857
small rodents as paratenic or intermediate hosts of carnivore parasites in berlin, germany.rodents are important intermediate and paratenic hosts for carnivore parasites, including the important zoonotic agents toxoplasma, echinococcus and toxocara. monitoring of such parasites in rodents can be used to detect increasing risks for human and veterinary public health. rodents were trapped at four sites in berlin, two near the city center, two at the periphery. pcrs were conducted to detect coccidia (target its-1) and specifically toxoplasma gondii (repetitive element) in brain and ascar ...201728278269
helminth infections and cardiovascular diseases: toxocara species is contributing to the disease.toxocariasis is the clinical term used to describe human infection with either the dog ascarid toxocara canis or the feline ascarid toxocara cati. as with other helminths zoonoses, the infective larvae of these toxocara species cannot mature into adults in the human host. instead, the worms wander through organs and tissues, mainly the liver, lungs, myocardium, kidney and central nervous system, in a vain attempt to find that, which they need to mature into adults. the migration of these immatur ...201727492228
toxocariasis of the central nervous system: with report of two cases.toxocariasis is a parasitic infection caused by the roundworms toxocara canis or toxocara cati, mostly due to accidental ingestion of embryonated eggs. clinical manifestations vary and are classified according to the organs affected. central nervous system involvement is an unusual complication. here, we report two cases with neurological manifestations, in which there was cerebrospinal fluid (csf) eosinophilia with marked blood eosinophilia and a positive serology for toxocara both in serum and ...201728157623
distribution and dynamics of soil contamination with toxocara canis and toxocara cati eggs in poland and prevention measures proposed after 20 years of study.the studies conducted in the years 1994-2013 allowed to determine the distribution of toxocara spp. eggs in the soil of different types of urban and rural areas in poland and to establish seasonal and multi-year dynamics of soil contamination with t. cati and t. canis eggs. out of 3309 soil samples examined, 14.9% contained the eggs and the average density was 3.43 eggs/100g of soil. the level of soil contamination was the highest in cities, lower in villages, and lowest in small towns. in urban ...201728115175
influence of land use and meteorological factors on the spatial distribution of toxocara canis and toxocara cati eggs in soil in urban areas.soil which has been contaminated by toxocara spp. eggs is considered as one of the main infection sources of toxocariasis in animals and humans. the present study conducted a detailed investigation into the spatial patterns of toxocara canis (t. canis) and toxocara cati (t. cati) eggs in soil in urban area of northeastern mainland china, and assessed the inter-relationships between meteorological factors, land use and the distribution of the toxocara spp. eggs. polymerase chain reaction (pcr) wa ...201728043392
genetic analysis of toxocara cati (nematoda: ascarididae) from guangdong province, subtropical china.toxocara cati (cat roundworm) is a common parasitic nematode that infects humans and other hosts, causing toxocariasis. although its significance as a pathogen, the epidemiology, genetics and biology of t. cati remain poorly understand in china. in the present study, genetic variation in mitochondrial (mt) cytochrome c oxidase subunit 1 (cox1) gene and internal transcribed spacer (its) of rdna region among t. cati in guangdong province, subtropical china was examined. a portion of the cox1 (pcox ...201728141958
first report of toxocara cati in the domestic land snail rumina decollata.the prospective role of the land snail rumina decollata as a potential paratenic host of toxocara cati for domestic cats was studied. r. decollata specimens and cats' feces were collected from the open spaces of a buenos aires city hospital. cats' feces were analyzed and snails were digested to identify t. cati stages, by morphological and molecular analyses. t. cati larval eggs were recovered from 23.5% (4/17) of the sampled feces. twenty percent of snail pools (5/25) were confirmed to be posit ...201727567520
road killed carnivores illustrate the status of zoonotic helminthes in caspian sea littoral of iran.carnivore carcasses on the roads can be regarded as study materials in parasitology and eco-epidemiology. stray carnivores such as dogs and cats are known to harbor so many different pathogens like zoonotic helminthes. the current investigation, apparent the status of the helminthic parasites found in road killed carnivores from different parts of guilan province north of iran.201728761483
abnormal neurobehaviour and impaired memory function as a consequence of toxocara canis- as well as toxocara cati-induced neurotoxocarosis.neuroinvasive larvae of the worldwide occurring zoonotic roundworms toxocara canis and t. cati may induce neurotoxocarosis (nt) in humans, provoking a variety of symptoms including cognitive deficits as well as neurological dysfunctions. an association with neuropsychological disorders has been discussed. similar symptoms have been described in t. canis-infected mice, whereas data on t. cati-induced nt are rare. therefore, it was aimed to obtain insights into the impact on neurobehaviour as well ...201728481889
endoparasites of eurasian lynx (lynx lynx) (linnaeus, 1758) from an enclosure of western pomeranian nature society in jablonowothe aim of this study was to describe parasites of three lynx living in an enclosure of western pomeranian nature society in jablonowo. during analysis of 3 gram faecal samples eggs of toxascaris leonina, toxocara cati, ancylostoma sp. and oocysts of cystoisospora felis were found. to our knowledge this is the first report of c. felis infection in lynx from poland. presented research show that wild cats in captivity are particularly exposed on parasitic infections and demand regular examination.201728601059
enzyme-linked immunosorbent assays for coproantigen detection of ancylostoma caninum and toxocara canis in dogs and toxocara cati in cats.we report the development and field validation of 2 elisas for the detection of ancylostoma caninum or toxocara canis coproantigens in the feces of dogs with experimental and natural infections, and evidence of cross-reactivity with respective feline counterparts. a. caninum-specific coproantigens were detected in feces of experimentally infected dogs starting at 9 d post-infection (dpi), whereas eggs were not seen until 23 dpi. t. canis-specific coproantigens were detected in 3 of 5 experimenta ...201728424002
gastrointestinal helminths and ectoparasites in the stray cats (felidae: felis catus) of ahar municipality, northwestern iran.the stray cats are considered as the sources of emerging humans and domestic livestock pathogens and the zoonoses of public health importance. the present study was aimed to elucidate intestinal helminth infections and infestation with ectoparasites of the stray cats of ahar city, northwestern iran.201728761492
prevalence of toxoplasma gondii and other gastrointestinal parasites in domestic cats from households in thika region, kenya.gastrointestinal (git) parasites of domestic cats (felis catus) not only cause morbidity but are also potential zoonotic agents. the current study aimed at establishing the prevalence of git parasites in cats kept by households in thika region, kenya. fecal samples were collected randomly from 103 cats and analyzed for presence of parasites using standard parasitological methods. in descending order, the prevalence of the detected protozoa parasites was isospora spp. 43.7% (95% ci: 40.4-47%), cr ...201728691033
environmental contamination with toxocara spp. eggs in public parks and playground sandpits of greater lisbon, portugal.toxocarosis is a zoonotic parasitic disease transmitted from companion animals to humans. environmental contamination with toxocara eggs is considered to be the main source of human infections. in portugal, knowledge regarding the current situation, including density, distribution and environmental contamination by toxocara spp., is largely unknown. the present study investigated environmental contamination with toxocara spp. eggs, in soil and faecal samples collected from public parks and playg ...201728545900
gastrointestinal parasites in stray and shelter cats in the municipality of rio de janeiro, brazil.the increasingly urban nature of the population has led many people to choose independent pets, such as cats. this situation has also made it possible for these animals to be abandoned, thus increasing the numbers of cats on the streets and in shelters. these animals can act as a source of infection for other hosts. between 2014 and 2015, the frequency of gastrointestinal parasites in captive and stray cats in the municipality of rio de janeiro was analyzed. ninety-one fecal samples were collect ...201728678896
efficacy of a new spot-on formulation of selamectin plus sarolaner against ancylostoma tubaeforme and toxocara cati in cats.the efficacy of a new spot-on formulation of selamectin plus sarolaner for cats was evaluated against induced infections with ancylostoma tubaeforme (hookworm) and toxocara cati (roundworm). five laboratory studies were conducted using adult, purpose-bred cats. four of the studies were designed to evaluate efficacy of the combination against a. tubaeforme, the dose-limiting gastrointestinal nematode species for selamectin. in two of these studies non-interference between selamectin and sarolaner ...201728395755
treatment of larva migrans syndrome with long-term administration of albendazole.larva migrans syndrome is a food-borne parasitic disease in humans, caused by accidental ingestion of eggs or larvae of ascarid nematodes, namely, toxocara canis, toxocara cati, or ascaris suum, the roundworms commonly found in the intestines of dogs, cats and pigs respectively. when a patient is diagnosed as having larva migrans syndrome, oral-administration of albendazole is recommended, however, the regimen remains controversial worldwide. in japan, the duration of albendazole administration ...201728754237
monoclonal antibody against toxocara cati second-stage larvae. 201728636499
assessment of u.s. pediatrician knowledge of toxocariasis.toxocariasis, one of a group of parasitic diseases known as neglected parasitic infections, is a disease caused by the larvae of two species of toxocara roundworms, toxocara canis, from dogs, and less commonly toxocara cati, from cats. although most infected individuals are asymptomatic, clinical manifestations may include fever, fatigue, coughing, wheezing, or abdominal pain (visceral toxocariasis) or vision loss, retina damage, or eye inflammation (ocular toxocariasis). to assess u.s. pediatri ...201728820703
the 33.1 kda excretory/secretory protein produced by toxocara canis larvae serves as a potential common biomarker for serodiagnosis of toxocariasis in paratenic animals and human.toxocariasis is a prevalent zoonosis disease caused by the closely related nematode species toxocara canis and toxocara cati which parasitise canidae and felidae respectively. in paratenic hosts, larvae of these worms cause multiple organ damage. however, how these paratenic hosts response to these worms and whether any common biomarker can be applied for diagnosis are still unclear.201728761463
[toxocariasis, a neglected disease in switzerland ?]toxocariasis is a zoonotic disease that occurs when humans are infected by the parasites toxocara canis or toxocara cati. toxocariasis is highly prevalent in tropical areas, but can also be found in industrialized countries, particularly in rural areas. clinical presentation can range from asymptomatic infection to more severe disease. diagnosis of toxocariasis is based on suggestive clinical findings, eosinophilia and a positive serological testing. albendazole represents the first-line treatme ...201728727331
differentiation of toxocara canis and toxocara cati based on pcr-rflp analyses of rdna-its and mitochondrial cox1 and nad1 regions.the definitive genetic identification of toxocara species is currently based on pcr/sequencing. the objectives of the present study were to design and conduct an in silico polymerase chain reaction-restriction fragment length polymorphism method for identification of toxocara species. in silico analyses using the dnasis and nebcutter softwares were performed with rdna internal transcribed spacers, and mitochondrial cox1 and nad1 sequences obtained in our previous studies along with relevant sequ ...201728682773
toxocara cati (nematoda: ascarididae) in didelphis albiventris (marsupialia: didelphidae) from brazil: a case of pseudoparasitism.eggs of toxocara cati were found in the feces of didelphis albiventris from a peridomestic urban environment in brazil. negative fecal tests following short-term captivity of the opossums, as well as the absence of ascaridids during necropsy, suggest the occurrence of pseudoparasitism. implications of the findings for the epidemiology of toxocariasis are discussed.201725517533
modified method to enhanced recovery of toxocara cati larvae for the purposes of diagnostic and therapeutic.human toxocariasis, extraintestinal-migration of toxocara species, is a worldwide helminthic zoonosis in many places of the undeveloped countries. toxocara cati is one of the common helminths in cats and it is a potentially preventable disease. its diagnosis and treatment depend on the demonstration of specific excretory-secretory toxocara antibodies from toxocara larvae by immunological assays. this study provides a simple manual technique which can be performed in any laboratory for recovering ...201627502936
evaluation of toxocara cati excretory-secretory larval antigens in serodiagnosis of human toxocariasis.toxocariasis is the clinical term that is applied to infection in the human host with toxocara species larvae. serological tests are important tools for the diagnosis of toxocariasis. the aim of this study was to evaluate the excretory-secretory (es) antigens of t. cati larvae using enzyme-linked immunosorbent assay (elisa) and also western blotting for serodiagnosis of human toxocariasis.201625846840
a cross-sectional study of tritrichomonas foetus infection in feral and shelter cats in prince edward island, canada.a cross-sectional study examined the occurrence of tritrichomonas foetus, and other intestinal parasites, in feral and shelter cats in prince edward island (pei). fecal samples were collected from 100 feral cats, 100 cats from the pei humane society, and 5 cats from a private residence. the occurrence of t. foetus, based on fecal culture, was 0% in feral and shelter cats. a single positive sample was obtained from an owned abyssinian cat that was imported to pei. intestinal parasites were identi ...201626933262
prevalence and risk factors for patent toxocara infections in cats and cat owners' attitude towards deworming.the prevalence of and risk factors for shedding toxocara eggs in cats older than 6 months were determined by examining 670 faecal samples collected in 4 cross-sectional studies in the netherlands. additionally, cat owners provided information on their attitude towards routine deworming. samples were examined using the centrifugal sedimentation flotation method. overall toxocara prevalence was 7.2 %. multivariable logistic regression analysis revealed that young age and living in rural areas were ...201627637227
a rare cause of hypereosinophilia: a case report.toxocariasis is a parasitic disease caused by the larval stage of toxocara cati and t. canis, which live in the intestinal system of cats (t. cati) and dogs (t. canis). infective eggs can enter the gastrointestinal system by the oral route via foods contaminated with feces of dogs or cats or via dirty contaminated hands. the larvae penetrate the small intestine and migrate to visceral organs by systemic circulation. hypereosinophilia is a common finding in the tissue invasion of parasites. serol ...201627594294
detection of specific antibody reactivity to toxocara larval excretory-secretory antigens in asthmatic patients (5-15 years).humans act as an intermediate host for toxocara canis and toxocara cati. toxocara may be an important risk factor for asthma in humans. the aim of the present study was to evaluate immunoglobulin g (igg) anti-toxocara canis antibody, using enzyme-linked immunosorbent assay (elisa) in asthmatic patients (aged 5-15 years), referring to a clinic of pulmonary diseases in arak, iran.201627857820
toxocara malaysiensis infection in domestic cats in vietnam--an emerging zoonotic issue?toxocara canis of canids is a parasitic nematode (ascaridoid) that infects humans and other hosts, causing different forms of toxocariasis. this species of toxocara appears to be the most important cause of human disease, likely followed by toxocara cati from felids. although some studies from malaysia and china have shown that cats can harbor another congener, t. malaysiensis, no information is available about this parasite for other countries. moreover, the zoonotic potential of this parasite ...201626584512
frequency of gastrointestinal parasites in cats seen at the university of são paulo veterinary hospital, brazil.the frequency of gastrointestinal infections in 502 cats seen at the veterinary hospital of the university of são paulo, sp, brazil, between 2005 and 2014, was measured. the samples were analyzed using methods of flotation and sedimentation. the results were compared with those from studies published previously using fecal samples from the same hospital at different times. associations between the frequency of positivity for each parasite and age, breed, sex, diarrhea and use of anthelmintic wer ...201627925073
helminth infections and gut microbiota - a feline perspective.investigations of the relationships between the gut microbiota and gastrointestinal parasitic nematodes are attracting growing interest by the scientific community, driven by the need to better understand the contribution of parasite-associated changes in the composition of the gut flora to both host malnutrition and immune modulation. these studies have however been carried out mainly in humans and experimental animals, while knowledge of the make-up of the gut commensal flora in presence or ab ...201627912797
genetic variability and discrimination of low doses of toxocara spp. from public areas soil inferred by loop-mediated isothermal amplification assay as a field-friendly molecular tool.aim: one of the main diagnostic problems of conventional polymerase chain reaction (pcr) is indiscrimination of low parasitic loads in soil samples. the aim of this study is to determine the genetic diversity and identification of toxocara spp. from public areas soil inferred by loop-mediated isothermal amplification (lamp) assay.201628096624
the occurrence of toxocara species in naturally infected broiler chickens revealed by molecular approaches.consuming raw and undercooked meat is known to enhance the risk of human toxocariasis because toxocara species have a wide range of paratenic hosts, including chickens. the aim of this study was to identify species of toxocara in naturally infected broiler chickens using molecular approaches. a polymerase chain reaction (pcr) method was used for the differentiation of toxocara canis and toxocara cati larvae recovered from tissues and organs, and identified by microscopic observations. thirty-thr ...201627571878
chronic polyarthritis as isolated manifestation of toxocariasis.human toxocariasis is a parasitic zoonosis mainly caused by toxocara canis or toxocara cati and is acquired by ingestion of the parasite's embryonated eggs. arthralgia and/or arthritis were reported in up to 17% of the cases, generally with acute duration (less than 6 weeks). however, to our knowledge, chronic polyarthritis, as the isolated presentation of toxocara infection, was not reported. one of the 5809 patients that was followed up at our service (0.017%) had chronic polyarthritis as the ...201627267534
eosinophilic myocarditis due to toxocariasis: not a rare cause.myocarditis is a clinically important disease because of the high mortality. from the perspective of treatment strategy, eosinophilic myocarditis should be distinguished from other types of myocarditis. toxocariasis, caused by toxocara canis or toxocara cati, is known as a cause of eosinophilic myocarditis but is considered rare. as it is an unpopular disease, eosinophilic myocarditis due to toxocariasis may be underdiagnosed. we experienced two cases of eosinophilic myocarditis due to toxocaria ...201627123346
evaluation of the goldmann-witmer coefficient in the immunological diagnosis of ocular toxocariasis.ocular toxocariasis (ot) is a zoonotic parasitic infection mainly caused by the intraocular tissue invasion of second-stage toxocara canis or toxocara cati larva. measuring specific anti-t. canis antibodies in the intraocular fluid (if) can increase ot diagnosis accuracy using goldmann-witmer coefficient (gwc). however, there is no systemic evaluation of gwc application in the immunological diagnosis of ot. to assess gwc for the immunodiagnosis of ocular toxocariasis, paired if and serum samples ...201626899678
risk factors for toxocara spp. seroprevalence and its association with atopy and asthma phenotypes in school-age children in a small town and semi-rural areas of northeast brazil.toxocara canis, toxocara cati, are roundworms that live in the intestines of dogs and cats, respectively, and are predominantly agents of human toxocariasis. studies have suggested that toxocara spp. seroprevalence increases levels of total and aeroallergen-specific ige (sige), asthma prevalence and asthma morbidity. nevertheless, other work reported a negative association between toxocara spp. seropositivity with skin hypersensititity and a positive association with sige. the objective of the p ...201627080332
innovative molecular diagnosis of trichinella species based on β-carbonic anhydrase genomic sequence.trichinellosis is a helminthic infection where different species of trichinella nematodes are the causative agents. several molecular assays have been designed to aid diagnostics of trichinellosis. these assays are mostly complex and expensive. the genomes of trichinella species contain certain parasite-specific genes, which can be detected by polymerase chain reaction (pcr) methods. we selected β-carbonic anhydrase (β-ca) gene as a target, because it is present in many parasites genomes but abs ...201626639312
the prevalence of intestinal nematodes in cats and dogs from lancashire, north-west england.to estimate prevalence of clinically-relevant intestinal nematodes in uk cats and dogs using the sensitive faecal analysis technique flotac.201627071856
flotation and adherence characteristics of toxocara canis and t. cati and a reliable method for recovering toxocara eggs from soil.toxocara canis and t. cati are worldwide distributed intestinal nematodes of canids and felids and pose a threat to public health due to possible clinical manifestations in humans. different methods for detection of toxocara eggs in soil have been described, but conducted studies deal with egg recovery rates of t. canis or "toxocara spp." only whereas t. cati egg recovery has not been taken into consideration. thus, flotation properties in sodium chloride solution and adherence characteristics t ...201627523935
optimal elisa antigen for the diagnosis of ascaris suum infection in humans.ascarid nematodes, ascaris suum, toxocara canis and toxocara cati, are the most important causative species of larva migrans syndrome (lms) in humans. although the diagnosis of ascarid lms is generally based on serological tests, specific serological tests for a. suum infection have not been fully developed. in the present study, the sensitivity and specificity of three a. suum antigen preparations, i.e., the somatic adult worm antigen (as-swap), larval excretory-secretory (es) antigens derived ...201627604123
ascarid infestation in captive siberian tigers in china.the siberian tiger is endangered and is listed by the international union for the conservation of nature; the captive environment is utilized to maintain siberian tiger numbers. little information regarding the prevalence of parasites in siberian tigers is available. a total of 277 fecal samples of siberian tigers were analyzed in this study. the microscopic analysis indicated the presence of ascarid eggs of toxascaris leonina and toxocara cati. the ascarid infection rate was 67.5% in siberian t ...201627514888
survey on parasitic infections in wildcat (felis silvestris silvestris schreber, 1777) by scat collection.wildcats are endangered felid species living in europe, asia, and africa. regrettably, scientific information on parasites of wildcats is particularly meager and they often rely on data gained by necropsies of a small number of animals. in the present study, scat collection was used to assess the parasite spectrum of european wildcats living in the etna park (sicily, italy). scat collection was performed from may to september 2010 by weekly walking four transects for a total of 391 km. samples w ...201626377843
seroepidemiology of human toxocara and ascaris infections in the netherlands.toxocara canis, toxocara cati and ascaris suum are worldwide-distributed zoonotic roundworms of dogs, cats and pigs, respectively. the epidemiology of these parasites in developed countries is largely unclear. two countrywide cross-sectional serosurveys were therefore conducted in the netherlands in 1995/1996 and 2006/2007 to investigate the prevalence, trends and risk factors for human toxocara and ascaris infections in the general population. the netherlands is characterized by high pig produc ...201627234034
gastrointestinal parasites of cats in brazil: frequency and zoonotic risk.gastrointestinal helminths are considered to be the most common parasites affecting cats worldwide. correct diagnosis of these parasites in animals living in urban areas is pivotal, especially considering the zoonotic potential of some species (e.g. ancylostoma sp. and toxocara sp.). in this study, a copromicroscopic survey was conducted using fecal samples (n = 173) from domestic cats living in the northeastern region of brazil. samples were examined through the flotac technique and the overall ...201627096530
development of a real-time pcr for a sensitive one-step coprodiagnosis allowing both the identification of carnivore feces and the detection of toxocara spp. and echinococcus multilocularis.studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. detecting and quantifying toxocara spp. and echinococcus multilocularis, two predominant zoonotic helminths circulating in european carnivores, in feces may help to better target measures for prevention. a rapid, sensitive, and one-step quantitative pcr (qpcr) allowing detection of e. multilocularis and toxocara spp. w ...201626969697
a hydroalcoholic extract from paullinia pinnata l. roots exerts anthelmintic activity against free-living and parasitic nematodes.paullinia pinnata is a medicinal plant traditionally used in west africa against a wide range of diseases including soil-transmitted helminthiases. in this study, a hydroethanolic root extract was investigated for its phytochemical composition and in vitro activity against the free-living nematode caenorhabditis elegans as well as the larval stages of the parasitic helminths ancylostoma caninum, haemonchus contortus, toxocara cati, and trichuris vulpis.lc-ms analysis of the ethanol-water (1 : 1) ...201627286336
a survey study on gastrointestinal parasites of stray cats in azarshahr, (east azerbaijan province, iran).gastrointestinal parasites are among the most common parasitic infections found in stray cats, which might act potential helminthic parasites to domestic cats. the objective of this study was to determine the prevalence of gastrointestinal parasites in stray cats in the city of azarshahr, which is located in east azerbaijan province, iran. a cross-sectional study was conducted on 50 necropsied stray cats, trapped and collected from different geographic regions of azarshahr. from a total 50 stray ...201627876926
concomitant infestation of toxocara cati and ancylostoma tubaeforme in a mongrel cat.a 3½ years old mongrel female cat was brought with the history of inappetence, seizure and lateral recumbency since 4 days and motion sickness since 2 days. faecal examination confirmed toxocara cati and ancylostoma tubaeforme along with un-hatched live toxocara cati larvae. treatment has been initiated with pyrantel pamoate and along with supportive therapy.201627065627
epidemiological survey of zoonotic helminths in feral cats in gran canaria island (macaronesian archipelago-spain).the presence of zoonotic parasites in feral cats have been widely considered all over the world. in gran canaria (macaronesian archipelago, canary islands, spain) the number of feral cats has grown out of control in urban and rural areas. 48 of felis catus captured in different gran canaria areas were studied. animals were necropsied and several organs were systematically examined in order to collect and identify macroscopic parasites. in addition, coprological tests were done in 28 cats. there ...201627447205
high prevalence of covert infection with gastrointestinal helminths in cats.fecal flotation is routinely used to identify feline helminth infections in clinical practice, but it is known to have limitations of sensitivity, particularly for cestodes. to determine the prevalence of helminths in a contemporary population of cats and evaluate the ability of fecal flotation to detect these infections, helminths were recovered from intestinal tracts removed from 116 adult cats humanely euthanized by an animal control shelter in northeastern oklahoma. results were compared to ...201526535453
new hosts and localities for helminths of carnivores in argentina.a total of 111 samples (43 faeces and 79 gastrointestinal tracts) of 14 wild carnivore species from 12 argentine provinces were analyzed. helminth eggs were identified in 73% of the faecal samples and adult worms were recovered from 81% of the gastrointestinal tracts. we found 19 helminth species. among the most frequent findings were parasites of domestic carnivores, namely toxocara canis, toxocara cati, toxascaris leonina, ancylostoma caninum, ancylostoma tubaeforme and uncinaria stenocephala. ...201526701468
development of a luminex bead based assay for diagnosis of toxocariasis using recombinant antigens tc-ctl-1 and tc-tes-26.the clinical spectrum of human disease caused by the roundworms toxocara canis and toxocara cati ranges from visceral and ocular larva migrans to covert toxocariasis. the parasite is not typically recovered in affected tissues, so detection of parasite-specific antibodies is usually necessary for establishing a diagnosis. the most reliable immunodiagnostic methods use the toxocara excretory-secretory antigens (tes-ag) in elisa formats to detect toxocara-specific antibodies. to eliminate the need ...201526485145
presence of anti-toxocara canis antibodies and risk factors in children from the amecameca and chalco regions of méxico.toxocariasis is a zoonotic disease that poses a threat to public health worldwide. this disease primarily affects children and is caused by the presence in the digestive tract of a common roundworm of dogs, toxocara canis, or cats, toxocara cati. toxocara is responsible for the presentation of various syndromes in humans depending on the affected organs.201526024905
gastrointestinal parasites of cats in denmark assessed by necropsy and concentration mcmaster technique.the large population of feral cats in denmark may potentially transmit pathogens to household cats and zoonotic parasites to humans. a total of 99 euthanized cats; feral cats (n=92) and household cats with outdoor access (n=7), were collected from march to may 2014 from the zealand region, denmark. the sedimentation and counting technique (sct) was used to isolate helminths and coproscopy was done by concentration mcmaster technique (c-mcmaster). overall, 90.1% of the cats were infected and a to ...201526169220
prevalence and risk factors of intestinal parasites in cats from china.the prevalence of intestinal parasites in cats from china was largely unknown prior to this study. the aim of the present study was to investigate the presence of intestinal parasites in cats from central china and also identify risk factors for parasitism. fecal samples from 360 cats were examined using sugar flotation procedure and fecal smear test by microscope. cats had mixed two or three kinds of parasites infections. of the 360 cats feces, intestinal parasites positive feces were 149 (41.3 ...201526078975
sequence variation in mitochondrial cox1 and nad1 genes of ascaridoid nematodes in cats and dogs from iran.the study was conducted to determine the sequence variation in two mitochondrial genes, namely cytochrome c oxidase 1 (pcox1) and nadh dehydrogenase 1 (pnad1) within and among isolates of toxocara cati, toxocara canis and toxascaris leonina. genomic dna was extracted from 32 isolates of t. cati, 9 isolates of t. canis and 19 isolates of t. leonina collected from cats and dogs in different geographical areas of iran. mitochondrial genes were amplified by polymerase chain reaction (pcr) and sequen ...201524717402
enteric parasites of free-roaming, owned, and rural cats in prairie regions of canada.the objective of this study was to determine prevalence, intensity, and zoonotic potential of gastrointestinal parasites in free-roaming and pet cats in urban areas of saskatchewan (sk) and a rural region in southwestern alberta (ab). fecal samples were analyzed using a modified double centrifugation sucrose flotation to detect helminth eggs and coccidian oocysts, and an immunofluorescence assay to detect giardia and cryptosporidium. endoparasite prevalence was higher in samples from rural ab ca ...201525969584
association between toxocariasis and cognitive function in young to middle-aged adults.the ascarid nematodes toxocara canis (werner, 1782) and toxocara cati (schrank, 1788) may infect humans resulting in toxocariasis. a prior study associated species of toxocara stiles, 1905 with cognitive deficits in children. to determine if a similar association between toxocariasis and cognition exists in adults, we analysed a large dataset from the united states' center for disease control's national health and nutrition examination survey. we used linear-regression and multivariate models to ...201526374832
toxocariasis: a rare cause of multiple cerebral infarction.toxocariasis is a parasitic infection caused by the roundworms toxocara canis or toxocara cati, mostly due to accidental ingestion of embryonated eggs. clinical manifestations vary and are classified as visceral larva migrans or ocular larva migrans according to the organs affected. central nervous system involvement is an unusual complication. here, we report a case of multiple cerebral infarction and concurrent multi-organ involvement due to t. canis infestation of a previous healthy 39-year-o ...201526157596
differential serodiagnostics of toxocara canis and toxocara cati--is it possible?one of the most common zoonotic helminth infections is caused by species in the genus toxocara, particularly toxocara canis and t. cati (syn. t. mystax). however, their relative contribution to toxocarosis in humans remains largely unknown because causative larvae are seldom recovered and uncertainties regarding the validity of existing serological assays. in this study, we used sera from a pig model experimentally infected with t. canis and t. cati to evaluate whether a western blot could discr ...201525711956
effects of toxocara larvae on brain cell survival by in vitro model assessment.neuroinvasive larvae of the common dog and cat roundworms, toxocara canis and toxocara cati, may cause severe neurological and neuropsychological disturbances in humans. despite their pathogenic potential and high prevalence worldwide, little is known about their cell-specific influences and cerebral host-pathogen interactions in neurotoxocarosis. to address this discrepancy, a co-culture system of viable larvae with murine neuronal (cad), oligodendrocytal (bo-1) and microglial (bv-2) cell lines ...201526080924
production of toxocara cati tes-120 recombinant antigen and comparison with its t. canis homolog for serodiagnosis of toxocariasis.toxocariasis is a cosmopolitan zoonotic disease caused by the infective larvae of toxocara canis and t. cati. diagnosis in humans is usually based on clinical symptoms and serology. immunoglobulin g (igg)-enzyme-linked immunosorbent assay kits using t. canis excretory-secretory (tes) larval antigens are commonly used for serodiagnosis. differences in the antigens of the two toxocara species may influence the diagnostic sensitivity of the test. in this study, t. cati recombinant tes-120 (rtes-120 ...201526033026
field evaluation of a rapid diagnostic test to detect antibodies in human toxocariasis.human toxocariasis which is caused mainly by the larvae of toxocara canis and toxocara cati, is a worldwide zoonotic disease that can be a potentially serious human infection. the enzyme-linked immunosorbent assay (elisa) using t. canis excretory-secretory (tes) antigens harvested from t. canis larvae is currently the serological test for confirming toxocariasis. an alternative to producing large amounts of toxocara tes and improved diagnosis for toxocariasis is through the development of highly ...201525910623
enhanced resolution of eosinophilic liver abscess associated with toxocariasis by albendazole treatment.visceral larva migrans, caused by toxocara canis and toxocara cati, has emerged as a significant cause of eosinophilic liver abscess (ela). differentiation of ela associated with toxocariasis (ela-t) from metastasis or primary liver malignancy is sometimes difficult. however, the role of albendazole treatment remains uncertain in this condition. the aim of this study was to evaluate whether albendazole can enhance the radiologic resolution of ela-t.201525896156
microarray gene expression analysis reveals major differences between toxocara canis and toxocara cati neurotoxocarosis and involvement of t. canis in lipid biosynthetic processes.toxocara canis and toxocara cati are globally occurring intestinal nematodes of dogs and cats with a high zoonotic potential. migrating larvae in the cns of paratenic hosts, including humans, may cause neurotoxocarosis resulting in a variety of neurological symptoms. toxocara canis exhibits a stronger affinity to the cns than t. cati, causing more severe neurological symptoms in the mouse model. pathomechanisms of neurotoxocarosis as well as host responses towards the respective parasite are mos ...201525843806
epidemiology of human toxocariasis in poland - a review of cases 1978-2009.toxocariasis is a helminthozoonosis due to the infection of humans with larvae belonging to the toxocara genus. humans become infected as a result of accidental consumption of infected eggs containing third stage larvae (l3) nematodes from toxocara canis or toxocara cati species. toxocariasis was recognized for the first time in the early 1950s, and the first cases of toxocariasis in poland were described a few years later. toxocariasis is clinically classified into several types: classic and in ...201525780823
neurotoxocarosis alters myelin protein gene transcription and expression.neurotoxocarosis is an infection of the central nervous system caused by migrating larvae of the common dog and cat roundworms (toxocara canis and toxocara cati), which are zoonotic agents. as these parasites are prevalent worldwide and neuropathological and molecular investigations on neurotoxocarosis are scare, this study aims to characterise nerve fibre demyelination associated with neurotoxocarosis on a molecular level. transcription of eight myelin-associated genes (cnp, mag, mbp, mog, mrf- ...201525773181
behavioural changes and muscle strength in rattus norvegicus experimentally infected with toxocara cati and t. canis.toxocara canis and toxocara cati are nematode parasites in dogs and cats, respectively, transmitted by ingestion of embryonated eggs, transmammary and transplacental (t. canis) routes and paratenic host predation. many parasites use mechanisms that change the behaviour of their hosts to ensure continued transmission. several researchers have demonstrated behavioural changes in mouse models as paratenic hosts for t. canis. however, there have been no studies on behavioural changes in laboratory r ...201524725503
the prevalence of trichuris spp. infection in indoor and outdoor cats on st. kitts.the present study was conducted to evaluate the prevalence of trichuris spp. and other intestinal parasitic infections in owned cats on st. kitts.201525596580
stray animal and human defecation as sources of soil-transmitted helminth eggs in playgrounds of peninsular malaysia.soil contaminated with helminth eggs and protozoan cysts is a potential source of infection and poses a threat to the public, especially to young children frequenting playgrounds. the present study determines the levels of infection of helminth eggs in soil samples from urban and suburban playgrounds in five states in peninsular malaysia and identifies one source of contamination via faecal screening from stray animals. three hundred soil samples from 60 playgrounds in five states in peninsular ...201525273274
experimental infection with toxocara cati in pigs: migratory pattern and pathological response in early phase.experimental inoculations of approximately 100,000 infective toxocara cati larval eggs were done in twelve pigs. the t. cati eggs used for inoculation were collected from cat's feces. another group of three pigs served as an uninfected control. groups of infected pigs were euthanized at seven, 14, 21, and 28 days post-inoculation (dpi). tissue samples were taken for digestion and histopathology changes in early phase. the number of larvae recovered from the lungs peaked at seven and 14 dpi and w ...201525076437
prevention of lactogenic toxocara cati infections in kittens by application of an emodepside/praziquantel spot-on (profender®) to the pregnant queen.this study aimed to evaluate the efficacy of an emodepside 2.1 % (w/v)/praziquantel 8.6 % (w/v) topical solution (profender® spot-on for cats) in the prevention of lactogenic toxocara cati infections. a controlled test was performed with two groups of 8 cats with confirmed pregnancy. all cats were infected with daily doses of 2000 t. cati eggs for 10 consecutive days starting 50 days post conception to produce an acute infection. treatment was performed 60 days post conception. queens in the tre ...201526152418
a toxocara cati eggs concentration method from cats' faeces, for experimental and diagnostic purposes.toxocariosis is a zoonotic parasite infection worldwide distributed, now considered a neglected disease associated to poverty. for experimental infection in animals and to develop the diagnosis in humans it is necessary to obtain large number of toxocara spp. larval eggs. toxocara cati eggs recovered percentage from faeces of infected cats was determined employing a novel egg concentration method. the mcmaster egg counting technique and the concentration method were applied on 20 positive cats' ...201424959943
toxocara cati larvae in the eye of a child: a case report.toxocariasis is a consequence of human infection by toxocara larvae. there are symptomatic (visceral, ocular) and asymptomatic course of toxocariasis. the ocular form is very rare. we present a 6-year-old patient who developed an ocular form of toxocariasis caused by toxocara cati. he demonstrated lesions in the peripheral retina of the right eye. white granuloma was present in the superior peripheral retina. a positive immunological assay for toxocariasis essentially completed the outcomes. on ...201425183145
helminths in feline coprolites up to 9000 years in the brazilian northeast.the identification of parasites in animal coprolites has been an important tool to promote knowledge about parasites infecting different zoological groups in the past. it also helps the understanding of parasites causing zoonoses, which is especially important for animals that were part of the diet of prehistoric human groups. nevertheless, the study of feline coprolites is still scarce. this study analyzed 30 feline coprolites from southeastern piauí taken from archeological sites used by human ...201425124856
efficacy of a novel topical combination of fipronil, (s)-methoprene, eprinomectin and praziquantel against adult and larval stages of toxocara cati in cats.the efficacy of a novel topical combination of fipronil 8.3% (w/v), (s)-methoprene 10% (w/v), eprinomectin 0.4% (w/v), and praziquantel 8.3% (w/v) (broadline(®), merial) was evaluated against adult and larval toxocara cati in four controlled studies. all studies included experimentally infected, purpose-bred, short-haired cats. in two studies, 22 or 20 cats harbouring patent infections as confirmed by pre-treatment faecal examination, were included. within each study, cats were allocated to one ...201424703074
human toxocariasis: current advances in diagnostics, treatment, and interventions.toxocariasis is a neglected zoonosis caused by the nematodes toxocara canis and toxocara cati. this disease is widespread in many countries, reaching high prevalence independently of the economic conditions. however, the true number of cases of toxocariasis is likely to be underestimated owing to the lack of adequate surveillance programs. although some diagnostic tests are available, their sensitivity and specificity need to be improved. in addition, treatment options for toxocariasis are limit ...201425089038
molecular characterization of toxocara spp. from soil of public areas in ahvaz southwestern iran.in the present study, the microscopy and polymerase chain reaction methods were used for detection and identification of soil contamination by toxocara eggs in squares, streets, public parks, and rubbish dumps in ahvaz, southwestern iran. a total of 210 soil samples were collected from different parts of the city and examined by microscopy and polymerase chain reaction (pcr) methods, following sodium nitrate flotation. nucleotide sequencing was performed to confirm the results of the pcr method. ...201424695242
detection of toxocara eggs in contaminated soil from various public places of chennai city and detailed correlation with literature.toxocarosis is one of the most prevalent human helminthosis caused by larvae of toxocara canis and toxocara cati, the most widely distributed nematode parasites of dogs and cats respectively. soil is considered as the principal source of transmission of toxocara infection to human beings. with increasing population of dogs and cats, soil contamination with ova or eggs of toxocara can be detected in public and private locations of city backyards, playgrounds, streets, sand pits and so on, regardl ...201424808647
toxocara seroprevalence among clinically healthy individuals, pregnant women and psychiatric patients and associated risk factors in shandong province, eastern china.toxocarosis is a widespread zoonosis caused by the ascarid nematodes toxocara canis and toxocara cati, which primarily infect dogs and cats, respectively. most human infections with toxocara are asymptomatic; however, some infected individuals may develop a serious illness and even death. nevertheless, epidemiological knowledge regarding the prevalence and risks associated with toxocara infection is limited in china. therefore, we performed a cross-sectional pilot study and estimated the seropre ...201425101756
toxocarosis and putative dress syndrome in an oncological patient: a case report.toxocarosis is a parasitic infection caused by toxocara canis or toxocara cati. their definite hosts are the domestic dog and cat, where their adult forms live within the lumen of the small intestine. in humans, infective larvae hatch after ingestion of eggs, but the juvenile stages fail to develop into mature adult worms. instead, they migrate through the body with the potential to affect virtually every body site. dress syndrome (drug reaction with eosinophilia and systemic symptoms) is a seri ...201424249317
modelling potential presence of metazoan endoparasites of bobcats (lynx rufus) using verified records.helminth parasites of wild and domestic felines pose a direct or potential threat to human health. since helminths depend on multiple environmental factors that make their transmission possible, it is imperative to predict the areas where these parasites may complete the transmission to potential hosts. bobcats, lynx rufus (schreberer), are the most abundant and widely-distributed wild felid species in north america. the increase of population densities of bobcats raises concerns about their imp ...201425549497
epizootic and zoonotic helminths of the bobcat (lynx rufus) in illinois and a comparison of its helminth component communities across the american midwest.a total of 6257 helminths of 19 taxa were recovered from the digestive tract and lungs of 67 bobcats in illinois. infections caused by alaria mustelae, diphyllobothrium latum, and macracanthorhynchus ingens are reported for the first time in bobcats. from all the taxa recovered, only three species occurred in high prevalence and caused intense infections: taenia rileyi, alaria marcianae, and toxocara cati, with prevalence and mean intensity of 70% and 6; 42% and 193, and 25% and 14 individuals, ...201424521984
efficacy of a novel topical fipronil, (s)-methoprene, eprinomectin and praziquantel combination against naturally acquired intestinal nematode and cestode infections in cats.the efficacy of a novel topical combination formulation of fipronil, (s)-methoprene, eprinomectin and praziquantel against naturally acquired intestinal nematode and cestode infections in cats was evaluated in seven negative control, blinded studies. cats were selected based on a pre-treatment faecal examination indicating a patent infection with at least hookworms (two studies), toxocara ascarids (one study), taeniid cestodes (two studies) or dipylidium cestodes (two studies). in each study, ca ...201424703071
parasitic infections detected by flotac in zoo mammals from warsaw, poland.the aim of this study was to estimate the occurrence of intestinal parasites in groups of mammals kept in the warsaw zoological garden (poland). 71 pools of fecal samples were analyzed using the flotac techniques. 48% of animals were positive and 47% of positivities showed multiple infections. toxocara cati (71.4%) was found in felines; marsupials were infected with coccidia (90%). giardia spp. (24.0%), blastocystis spp. (12.3%), iodamoeba spp. (10.0%), enterobius vermicularis (6.0%) and entamoe ...201424827109
current status of l. infantum infection in stray cats in the madrid region (spain): implications for the recent outbreak of human leishmaniosis?since 2009, the incidence of human leishmaniosis in the sw of the madrid region has been unusually high. although dogs are the main reservoir for this disease, a role played by dogs in this outbreak has been ruled out and investigators are now considering other hosts (eg. cats, rabbits, hares) as possible alternative reservoirs.this study was designed to examine the leishmania infantum status of stray cats in madrid to assess its possible implications in the human leishmaniosis outbreak.201424655361
parasite communities in stray cat populations from lisbon, portugal.stray cats live in high-density colonies in urban areas and pose a health hazard to household cats and humans. in portugal, information on the parasitic fauna of stray cats is limited and relies mostly on results from faecal analysis. the present survey aimed to determine the prevalence, diversity and intensity of parasites in stray cats from the urban area of lisbon by means of parasitological necropsy. internal organs were collected from 162 cats captured in different areas of the city and sys ...201423719370
expect the unexpected: a case of isolated eosinophilic meningitis in toxocariasis.we present the case of a young police officer suffering from headache without other neurological symptoms caused by isolated eosinophilic meningitis, which resulted from an infection with toxocara cati, along with a discussion of the differential diagnosis.201425535488
parasites of domestic owned cats in europe: co-infestations and risk factors.domestic cats can be infested by a large range of parasite species. parasitic infestations may cause very different clinical signs. endoparasites and ectoparasites are rarely explored in the same study and therefore multiparasitism is poorly documented. the present survey aimed to improve knowledge of the prevalence and risk factors associated with ecto- and endoparasite infestations in owned cats in europe.201424965063
survey of helminth parasites of cats from the metropolitan area of cuiabá, mato grosso, brazil.besides presenting zoonotic potential, helminths of cats are responsible for gastrointestinal, hepatic, and pulmonary diseases. in order to identify the helminthic fauna, prevalence, mean intensity of parasitism (mip), and mean abundance population (map), 146 cats from the metropolitan area of cuiabá, midwestern brazil, were necropsied. in 98 these animals, 12 species of helminths were identified, comprising (species, prevalence, mip, and map, respectively): nematodes (ancylostoma braziliense [5 ...201323856737
prevalence of faecal-borne parasites in colony stray cats in northern italy.endoparasitic infections are common in stray cats. many of these parasites are responsible for zoonoses, and stray cats can be a source of environmental contamination. the prevalence of parasites in 139 stray colony cats in the city of milan, northern italy, was investigated by faecal examination. the overall prevalence of endoparasites was 50.4%, with 11 different parasites found. parasites with zoonotic potential were detected in 49.6% of cats. concurrent infections with two or more zoonotic p ...201323329613
discrimination of gastrointestinal nematode eggs from crude fecal egg preparations by inhibitor-resistant conventional and real-time pcr.diagnosis of gastrointestinal nematodes relies predominantly on coproscopic methods such as flotation, kato-katz, mcmaster or flotac. although flotac allows accurate quantification, many nematode eggs can only be differentiated to genus or family level. several molecular diagnostic tools discriminating closely related species suffer from high costs for dna isolation from feces and limited sensitivity since most kits use only small amounts of feces (<1 g). a direct pcr from crude egg preparations ...201323620739
cross transmission of gastrointestinal nematodes between captive neotropical felids and feral cats.pathogen cross transmission between wildlife and domestic animals represents an extinction risk for wildlife; however, reliable verification is difficult to perform, and in some cases, it is even considered unlikely to be conducted. the aim of this work was to identify cross transmission of helminths between feral cats and captive wild felids at a zoological park in southeastern mexico. feces were collected from jaguars (panthera onca), cougars (puma concolor), ocelots (leopardus pardalis), marg ...201324450052
natural infection by endoparasites among free-living wild animals.the objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living wild animals. fecal samples from wild mammals and birds at rehabilitation centers in the states of mato grosso do sul and são paulo were analyzed by sedimentation and flotation-centrifugation methods. parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. cryptosporidium sp. oocysts were detected in fecal samples from oncillas (leopardus ti ...201323778826
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