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prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower northern thailand.gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. we investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower northern thailand and utilized molecular tools for species identification of hookworms and opisthorchis viverrini. fecal samples of 197 dogs and 180 cats were collected. overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. helminth infection found in both dogs a ...201727570099
fish sharing as a risk factor for opisthorchis viverrini infection: evidence from two villages in north-eastern thailand.foodborne trematodiasis (fbt) is a significant global health problem, with the liver flukes opisthorchis viverrini, o. felineus, and clonorchis sinensis contributing to half of the global burden of fbt. north-eastern thailand where o. viverrini is endemic and un-cooked fish dishes remain an integral part of the food culture has the highest reported incidence of opisthorchiasis, including associated cholangiocarcinoma. both food sharing and eating practices are potentially important factors in ft ...201728372560
recurrent emergence of structural variants of ltr retrotransposon csrn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, clonorchis sinensis.autonomous retrotransposons, in which replication and transcription are coupled, encode the essential gag and pol genes as a fusion or separate overlapping form(s) that are expressed in single transcripts regulated by a common upstream promoter. the element-specific expression strategies have driven development of relevant translational recoding mechanisms including ribosomal frameshifting to satisfy the protein stoichiometry critical for the assembly of infectious virus-like particles. retrotra ...201728322871
clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin β4-fak/src signaling pathway.cholangiocarcinoma (cca) is a slow-growing but highly metastatic cancer. its metastatic potential largely explains its high mortality rate. a recognized risk factor for cca development is infection with the liver flukes opisthorchis viverrini and clonorchis sinensis. we previously reported that the excretory-secretory products (esps) of c. sinensis promoted the three-dimensional aggregation and invasion of cca cells. in the present study, a quantitative real-time pcr array of extracellular matri ...201728286026
[control of opisthorchis viverrini infection for cholangiocarcinoma prevention].the international agency for research on cancer (iarc) has classified two liver flukes as carcinogenic to humans (group 1): opisthorchis viverrini in 1994 and clonorchis sinensis in 2009. this review is focused on o. viverrini, the most studied of these two trematodes, which infects nearly 10 million people in southeast asia. the life cycle involves two intermediate hosts living in fresh water: a snail of the genus bithynia and a ciprinid fish. the definitive hosts (human, cat, dog) become infec ...201728105582
liver fluke infection and cholangiocarcinoma: a review.parasites are significant groups for carcinogenesis among which liver flukes, including opisthorchis viverrini and clonorchis sinensis, are typical representatives causing cholangiocarcinoma (cca), the second most common primary hepatic malignancy with dismal prognosis. o. viverrini is prevalent in southeast asia, infecting 10 million people while c. sinensis has a wider distribution in east asia and several southeast asian countries, affecting more than 35 million people's health. these two wor ...201727718017
modeling impacts of climate change on the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand.global climate change is now regarded as imposing a significant threat of enhancing transmission of parasitic diseases. maximum entropy species distribution modeling (maxent) was used to explore how projected climate change could affect the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand. a range of climate variables was used: the hadley global environment model 2-earth system (hadgem2-es) climate change model and also the ipcc scenarios a2a for 2050 a ...201727774575
delineating distinct heme-scavenging and binding functions of domains in mf6p/hdm proteins from parasitic flatworms.mf6p/fhhdm-1 is a small protein secreted by the parasitic flatworm (trematode) fasciola hepatica and belonging to a broad family of heme-binding proteins (mf6p/hdms). mf6p/hdms are of interest for understanding heme homeostasis in trematodes and also as potential targets for the development of new flukicides. moreover, interest in these molecules has also increased due to their immunomodulatory properties. here we have extended our previous findings on the mechanism of mf6p/hdm-heme interactions ...201728348084
experimental and modelling investigations of opisthorchis viverrini miracidia transmission over time and across temperatures: implications for control.transmissibility is a significant factor in parasite fitness. the rate and magnitude of parasite transmission affect prevalence and infection intensity in individual hosts and are influenced by environmental factors. in this context, the objectives of this study were: (i) to experimentally assess opisthorchis viverrini miracidia survival and infectivity over time and across temperatures; and (ii) to combine these experimental results with environmental data to build a key component of a transmis ...201728237890
differential protein expression marks the transition from infection with opisthorchis viverrini to cholangiocarcinoma.parts of southeast asia have the highest incidence of intrahepatic cholangiocarcinoma (cca) in the world due to infection by the liver fluke opisthorchis viverrini (ov). ov-associated cca is the culmination of chronic ov-infection, with the persistent production of the growth factors and cytokines associated with persistent inflammation, which can endure for years in ov-infected individuals prior to transitioning to cca. isobaric labelling and tandem mass spectrometry of liver tissue from a hams ...201728232516
role of socio-cultural and economic factors in cyprinid fish distribution networks and consumption in lawa lake region, northeast thailand: novel perspectives on opisthorchis viverrini transmission dynamics.opisthorchis viverrini (ov) is a fish-borne parasite endemic in parts of lao pdr, cambodia, southern vietnam and northeast thailand (isaan) where an estimated 10 million people are infected. human ov infection, associated with hepatobiliary complications, including cholangiocarcinoma (cca), occurs when infected fish are consumed raw or undercooked, a longstanding cultural tradition in the region. this mixed- methods descriptive study was carried out in isaan villages around lawa lake, khon kaen ...201728216369
elevated prevalence of helicobacter species and virulence factors in opisthorchiasis and associated hepatobiliary disease.recent reports suggest that opisthorchis viverrini serves as a reservoir of helicobacter and implicate helicobacter in pathogenesis of opisthorchiasis-associated cholangiocarcinoma (cca). here, 553 age-sex matched cases and controls, 293 and 260 positive and negative for liver fluke o. viverrini eggs, of residents in northeastern thailand were investigated for associations among infection with liver fluke, helicobacter and hepatobiliary fibrosis. the prevalence of h. pylori infection was higher ...201728198451
morphological and molecular identification of the liver fluke opisthorchis viverrini in the first intermediate host bithynia snails and its prevalence in kampong cham province, cambodia. 201728189767
application of environmental dna analysis for the detection of opisthorchis viverrini dna in water samples.opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in southeast asian countries. the trematode, opisthorchis viverrini, is the causative agent of the disease. accurate and rapid monitoring of o. viverrini is crucial for disease prevention and containment. therefore, in this study we sought to develop a novel species-specific real-time pcr assay for detecting o. viverrini using environmental dna (edna). the diagnostic sensitivity ...201728108370
new locality record for haplorchoides mehrai and possible interactions with opisthorchis viverrini metacercariae in cyprinid fishes in northeast thailand.metacercariae of opisthorchis viverrini, a carcinogenic liver fluke, and haplorchoides sp., a trematode maturing in catfish, are commonly found in cyprinid fish, the second intermediate hosts of both flukes. however, the specific identity of haplorchoides sp. in thailand and a precise assessment of the effects of co-infections with o. viverrini have never been clarified. therefore, we aimed to identify the species of haplorchoides and to investigate possible interactions of the two trematode spe ...201727882434
cytokine profiles in opisthorchis viverrini stimulated peripheral blood mononuclear cells from cholangiocarcinoma patients.the carcinogenic liver fluke opisthorchis viverrini causes chronic inflammation in the bile duct and resulting in unremitting tissue damage that lead to hepatobiliary diseases, including cholangiocarcinoma (cca). despite inflammatory cytokine expression having been studied in the animal model, so far no studies have been carried out on cytokines in human cca cases. here we report the profile of cytokine production in peripheral blood mononuclear cells (pbmcs) collected from o. viverrini-associat ...201727769807
nitrative and oxidative dna damage in infection-related carcinogenesis in relation to cancer stem cells.infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and nitrative dna lesions, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in inflammation-mediated carcinogenesis. it has bee ...201728163802
coinfection with helicobacter pylori and opisthorchis viverrini enhances the severity of hepatobiliary abnormalities in hamsters.persistent infection with opisthorchis viverrini causes hepatobiliary abnormalities, predisposing infected individuals to cholangiocarcinoma (cca). in addition, helicobacter pylori is highly prevalent in most countries and is a possible risk factor for cca; however, its role in enhancing hepatobiliary abnormality is unclear. here, we investigated the effects of coinfection with h. pylori and o. viverrini on hepatobiliary abnormality. hamsters were divided into four groups: (i) normal, (ii) h. py ...201728138021
efficacy of moxidectin versus ivermectin against strongyloides stercoralis infections: a randomized controlled non-inferiority trial.infections with strongyloides stercoralis are of considerable public health relevance. moxidectin, a well-established drug in veterinary medicine under consideration for regulatory submission for the treatment of onchocerciasis, might serve as alternative to the most widely used ivermectin.201728369530
opisthorchis viverrini infection in the snail and fish intermediate hosts in central vietnam.opisthorchis viverrini, a carcinogenic fish borne fluke, requires freshwater snails and fish as intermediate hosts. opisthorchiasis is endemic in parts of southeast asia, including central and south vietnam. in this region the transmission by intermediate hosts has received little attention. therefore, freshwater snails and wild fish from bau my tho, an opisthorchiasis endemic area in binh dinh province were collected for examination of o. viverrini cercariae and metacercariae, respectively. a t ...201728242064
preliminary genetic evidence of two different populations of opisthorchis viverrini in lao pdr.opisthorchis viverrini is a major public health concern in southeast asia. various reports have suggested that this parasite may represent a species complex, with genetic structure in the region perhaps being dictated by geographical factors and different species of intermediate hosts. we used four microsatellite loci to analyze o. viverrini adult worms originating from six species of cyprinid fish in thailand and lao pdr. two distinct o. viverrini populations were observed. in ban phai, thailan ...201728238124
effects of albendazole, artesunate, praziquantel and miltefosine, on opisthorchis viverrini cercariae and mature metacercariae.to explore larvicidal effects of anthelmintic drugs on opisthorchis viverrini (o. viverrini) for alternative approach to interrupting its cycle for developing a field-based control program.201728237476
social network analysis of food sharing among households in opisthorchiasis endemic villages of lawa lake, thailand.consumption of raw fish is a well-documented risk factor for opisthorchis viverrini infection. sharing of food, especially raw fish recipes may influence the spread of disease through a community. using social network analysis of an ego network, we investigated food sharing among households in an opisthorchis-endemic area. network centrality properties were used to explain the differences in o. viverrini transmission and control between villages with a low and high prevalence of infection. infor ...201728188767
efficacy and safety of praziquantel against light infections of opisthorchis viverrini: a randomized parallel single-blind dose-ranging trial. 201728174906
targeting hexokinase ii as a possible therapy for cholangiocarcinoma.overexpression of hexokinase 2 (hkii) has been demonstrated in various cancers. a number of in vitro and in vivo studies in several cancers show the significance of hkii in many cellular processes including proliferation, metastasis and apoptosis. however, the role of hkii in opisthorchis viverrini (ov) associated cholangiocarcinoma (cca) is still unknown. in the present study, the expression and roles of hkii were determined in ov associated cca. the expression of hkii was investigated in 82 pa ...201728131825
the complete mitochondrial genome of orthocoelium streptocoelium (digenea: paramphistomidae) for comparison with other digeneans.orthocoelium streptocoelium is a common paramphistome species parasitizing the rumen and/or reticulum of small ruminants, leading to significant losses. this study first determined the complete mitochondrial (mt) genome of o. streptocoelium. the complete mt genome of o. streptocoelium was amplified, sequenced, assembled, analysed and then compared with those of other digeneans. the entire mt genome of o. streptocoelium is 13,800 bp in length, which is smaller than those of other digeneans except ...201727030161
development of a pcr assay for diagnosing trematode (opisthorchis and haplorchis) infections in human stools.we developed a combined conventional polymerase chain reaction (pcr) and real-time pcr (qpcr)-based assay for detecting and discriminating between opisthorchis viverrini and haplorchis taichui parasite infections. the first pcr amplifies the mitochondrial cytochrome c oxidase subunit i (coi) genes of parasites, and differential diagnosis is achieved by performing qpcr with specific primers and sybr green i. the detection limit of the assay was found to be 2.0 × 10(2) plasmid copies in a test in ...201727821695
epidemiological investigation of parasitic infection of schoolchildren from six elementary schools in sakon nakhon province, thailand.we conducted an epidemiological study of intestinal parasitic infection in 572 schoolchildren aged 4 to 12 years old from six elementary schools in sakon nakhon province, thailand from june 2013 to august 2014. we collected fecal, blood, and urine samples to investigate parasitic infection and conducted a questionnaire survey. soil samples were examined for egg contamination. fecal examination, using the formalin-ether sedimentation method, revealed that 39% of schoolchildren were infected with ...201728289269
comparison of novel and standard diagnostic tools for the detection of schistosoma mekongi infection in lao people's democratic republic and cambodia.given the restricted distribution of schistosoma mekongi in one province in lao people's democratic republic (lao pdr) and two provinces in cambodia, together with progress of the national control programmes aimed at reducing morbidity and infection prevalence, the elimination of schistosomiasis mekongi seems feasible. however, sensitive diagnostic tools will be required to determine whether elimination has been achieved. we compared several standard and novel diagnostic tools in s. mekongi-ende ...201728793922
lineage-specific expansion and loss of tyrosinase genes across platyhelminths and their induction profiles in the carcinogenic oriental liver fluke, clonorchis sinensis.tyrosinase provides an essential activity during egg production in diverse platyhelminths by mediating sclerotization of eggshells. in this study, we investigated the genomic and evolutionary features of tyrosinases in parasitic platyhelminths whose genomic information is available. a pair of paralogous tyrosinases was detected in most trematodes, whereas they were lost in cyclophyllidean cestodes. a pseudophyllidean cestode displaying egg biology similar to that of trematodes possessed an ortho ...201728583212
codon usage patterns of tyrosinase genes in clonorchis sinensis.codon usage bias (cub) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. in this study, genetic indices associated with cub, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the clonorchis sinensis tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. the ...201728506040
first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar.opisthorchis viverrini is endemic in the south east asian region, especially in cambodia, lao people's democratic republic, vietnam and thailand, but there have been no previous records from myanmar. during stool surveys of rural populations in three regions of lower myanmar, opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. dna was extracted from these positive stool samples and a portion o ...201728472153
opisthorchiasis and cholangiocarcinoma in southeast asia: an unresolved problem.the prevalence of cholangiocarcinoma (cca) in southeast asia is much higher than other areas of the world. eating raw, fermented, or undercooked cyprinid fish, infected with the liver fluke, opisthorchis viverrini sensu lato (sl), results in chronic biliary inflammation, periductal fibrosis, and increased cancer risk. there may be associated glomerulonephritis. the process of infection is difficult to disrupt because eating practices have proven extremely difficult to change, and the life cycle ...201728848361
the battle of worldviews: a case study of liver fluke infection in khon kaen, thailand.control efforts to reduce infection from the parasitic flatworm opisthorchis viverrini have progressed through understanding the epidemiology of opisthorchis viverrini, antiparasitic drug developments, technological innovations, health education promoting cooking of fish, and improved hygienic defecation. yet the problem persists. the case study method was used to examine the fundamental cause of the liver fluke infection problem. evidence shows that the liver fluke-infected population does not ...201728795594
prospects and challenges towards sustainable liver fluke control.the liver fluke opisthorchis viverrini (ov) is endemic in southeast asia where more than 10 million people are estimated to be infected. the infection is associated with several hepatobiliary diseases, including cholangiocarcinoma (cca). northeast thailand is a hotspot for ov transmission, and, despite extensive public health prevention campaigns led by the government, the prevalence of ov infection is still high. high infection rates result from cultural and ecological complexities where wet-ri ...201728754415
ultrastructural characters of the spermatozoon of the liver fluke opisthorchis viverrini (poirier, 1886) (opisthorchiidae).the present study records the ultrastructural organization of the mature spermatozoon of opisthorchis viverrini by means of transmission electron microscopy. the spermatozoon of o. viverrini is a filiform cell, tapered at both extremities. it exhibits the characteristics of type iv spermatozoon of digeneans, namely with two axonemes of the 9+'1' trepaxonematan pattern, external ornamentation of the plasma membrane associated with cortical microtubules that are in the posterior part of the anteri ...201728725936
subsets of inflammatory cytokine gene polymorphisms are associated with risk of carcinogenic liver fluke opisthorchis viverrini-associated advanced periductal fibrosis and cholangiocarcinoma.opisthorchis viverrini infection induces chronic inflammation, and a minor proportion of infected individuals develop advanced periductal fibrosis (apf) and cholangiocarcinoma (cca). inflammatory cytokines and/or their gene polymorphisms may link to these biliary pathologies. we therefore investigated associations among cytokine gene polymorphisms and cytokine production in 510 thai cases infected with o. viverrini who presented with apf+ or apf-, as established by abdominal ultrasonography as w ...201728719954
simplified techniques for killing the carcinogenic, opisthorchis viverrini metacercariae in cyprinid fishconsumption of fluke-free fish is the most important factor in controlling opisthorchis viverrini (ov) infection in endemic areas such as northeast thailand and thereby reducing the risk of cholangiocarcinoma. cooking fish is the best way to avoid infection; however, the cultural practice of eating raw or fermented fish is difficult to change. we investigated the food preparation process, using freezing, heating and fermentation to kill ov metacercariae in fish. uncooked cyprinid fish infected w ...201728669159
present situation of opisthorchiasis in vientiane capital, lao peoples' democratic republic.opisthorchiasis is the commonest liver disease caused by opisthorchis viverrini in thailand, lao people's democratic republic (pdr) and cambodia, which will be a major cause of future human cholangiocarcinoma in these regions. in this short review, we describe the epidemic aspects of this parasite infection and the outline of results of our recent surveys of the fluke in humans and its intermediate hosts in vientiane capital, where information on the opisthorchiasis epidemic is insufficient. we ...201728552889
development of a potent wound healing agent based on the liver fluke granulin structural fold.granulins are a family of protein growth factors that are involved in cell proliferation. an orthologue of granulin from the human parasitic liver fluke opisthorchis viverrini, known as ov-grn-1, induces angiogenesis and accelerates wound repair. recombinant ov-grn-1 production is complex and poses an obstacle for clinical development. to identify the bioactive region(s) of ov-grn-1, four truncated n-terminal analogues were synthesized and characterized structurally using nmr spectroscopy. pepti ...201728425707
protein kinases as targets for opisthorchis viverrini- associated cholangiocarcinoma therapy.protein kinases are enzymes that catalyze the transfer of phosphate from atp to the serine/threonine or tyrosine residues of target molecules. these are key important mediators in a signaling cascade involved in several biological processes. dysregulation of their activity has been found in various tumors. from our increased understanding of kinase structure and activation mechanisms there emerged new strategies for targeting kinase in cancer treatment. nowadays, kinase specific inhibitors are d ...201728699535
nimotuzumab inhibits cholangiocarcinoma cell metastasis via suppression of the epithelial-mesenchymal transition process.changes in epidermal growth factor receptor (egfr) are commonly found in cancer progression, signaling a poor outcome in patients. in the present study, we aimed to investigate whether nimotuzumab could be of benefit for cholangiocarcinoma (cca) treatment.201728668850
the role of domestic dogs in the transmission of zoonotic helminthes in a rural area of mekong river basin.dogs have been bred since ancient times for companionship, hunting, protection, shepherding and other human activities. some canine helminth parasites can cause significant clinical diseases in humans as opisthorchis viverrini causing cholangiocarcinoma in southeast asian countries. in this study, socio-cultural questionnaire, canine parasitological analysis, necropsy, parasite molecular confirmation and dog roaming data were evaluated in savannakhet, lao-pdr, a typical mekong basin area. dog ow ...201728426425
serum antibody ig g and ig m titers for opisthorchis felineus correlate with eggs in faeces--a comprehensive study in chuvash republic, russia.the cholangiocarcinoma is a. the risk of development of cholangiocarcinoma, generally a rare type of a liver tumor, increases during infection of opisthorchiasis. for this reason the timely detection of opisthorchiasis is important for cholangiocarcinoma prevention. there are many studies which concern the detection of pathogenesis of opisthorchis viverrini infection but a little known about opisthorchis felineus. in this study we investigate a correlation of the eggs which are found in a faeces ...201626838224
genetic differences among haplorchis taichui populations in indochina revealed by mitochondrial cox1 sequences.haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. it is widely distributed in asia, with a particularly high prevalence in indochina. previous work revealed that the lack of gene flow between three distinct populations of vietnamese h. taichui can be attributed to their geographic isolation with no interconnected river basins. to test the hypothesis that interconnected river basins allow gene flow between otherwise isolated populations of h. taichui, as previous ...201627411962
opisthorchis viverrini infections and associated risk factors in a lowland area of binh dinh province, central vietnam.opisthorchiasis caused by opisthorchis viverrini is a major public health problem in the mekong basin in south east asia. it is associated with cholangiocarcinoma, a fatal cancer of the bile duct, which is very common in some areas of thailand and lao pdr. although there is evidence of opisthorchiasis in the central and southern provinces of vietnam, data are scarce and vietnam is often not considered an opisthorchiasis endemic area in the international literature. we conducted a cross-sectional ...201626872984
re-examination of opisthorchis viverrini in nakhon ratchasima province, northeastern thailand, indicates continued needs for health intervention.opisthorchis viverrini infection is associated with cholangiocarcinoma particularly in the cases of chronic or re-infection. this presents a serious health problem in northeastern and northern thailand. a community base approach is required for surveillance. therefore, in a pilot project, re-examination of o. viverrini infection was conducted in the 3 districts of nakhon ratchasima province, thailand, during june and october 2015. a total of 355 participants from a 194,152 population, was select ...201626838215
andrographolide ameliorates beta-naphthoflavone-induced cyp1a enzyme activity and lipid peroxidation in hamsters with acute opisthorchiasis.opisthorchis viverrini (ov) infection generates oxidative stress/free radicals and is considered as a primary cause ofcholangiocarcinoma since it primarily triggers sclerosing cholangitis.201626817235
comparison of infectivity, metacercarial burden and host mortality induced by opisthorchis viverrini sensu lato cercariae from lao pdr compared with thailand in cyprinid fish, barbonymus gonionotus.previous studies indicate that opisthorchis viverrini sensu lato (s.l.) is a species complex with strong associations to geographical regions, i.e., specific wetlands. the present study was conducted to compare the infectivity, establishment and metacercarial burden and survival of the fish host following exposure to o. viverrini s.l. cercariae isolates from two different river wetlands.201626740362
nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in opisthorchis viverrini-infected hamsters.this study investigated the effects of nanoencapsulated curcumin (nec) and praziquantel (pzq) treatment on the resolution of periductal fibrosis (pdf) and bile canalicular (bc) abnormalities in opisthorchis viverrini infected hamsters. chronic o. viverrini infection (ov) was initially treated with either pzq (op) and subsequently treated with nec (op+nec), curcumin (op+cur) or unloaded carriers (op+carrier) daily for one month. op+nec treatment reduced the pdf by suppression of fibrotic markers ...201626542278
diagnosis of opisthorchis viverrini infection with handheld microscopy in lao people's democratic republic.opisthorchiasis is a neglected tropical disease, yet it is of considerable public health importance in southeast asia given the predilection for chronically infected persons to develop cholangiocarcinoma. we evaluated a handheld microscope for the diagnosis of opisthorchis viverrini in a community-based setting in lao people's democratic republic in comparison with conventional light microscopy. in stool samples collected from 104 individuals, handheld microscopy revealed a sensitivity of 70.6% ...201626526923
combination of praziquantel and aspirin minimizes liver pathology of hamster opisthorchis viverrini infection associated cholangiocarcinoma.opisthorchiasis is one of the major risk factors for cholangiocarcinoma (cca) in northeastern thailand. an effective drug for killing this parasite is praziquantel. recently, several reports have shown that with frequent use, praziquantel may itself be a cca risk and can cause liver cell damage from an immunopathological response after parasite death. aspirin has many properties including anti-inflammation and anti-cancer. therefore, we use of aspirin (as) and praziquantel (pz) to improve hepato ...201626277607
suspected cases of cholangiocarcinoma seen in reference hospitals in lao people's democratic republic.cholangiocarcinoma (cca) poses a significant public health problem in southeast asia, with chronic opisthorchis viverrini infection being the main risk factor. epidemiological data on cca is not available for lao people democratic republic (lao pdr), though there is a high prevalence of o. viverrini infection in the country. a hospital record-based retrospective study was carried out in six referral hospitals (four in the capital city, one in savannakhet, one in pakse) to assess the number of su ...201627965165
glycoproteomics analysis of plasma proteins associated with opisthorchis viverrini infection-induced cholangiocarcinoma in hamster model.to apply lectin affinity chromatography and glycoproteomics-based lc-ms/ms to preliminarily investigate the possible potential plasma biomarkers of opisthorchis viverrini (ov)-associated cca in ov/dimethylnitrosamine (dmn)-induced cca hamster model.201627955744
uncovering the pathogenic landscape of helminth (opisthorchis viverrini) infections: a cross-sectional study on contributions of physical and social environment and healthcare interventions.helminth infections have proven recalcitrant to control by chemotherapy in many parts of southeast asia and indeed farther afield. this study isolates and examines the influence of different aspects of the physical and social environment, and uneven intervention effort contributing to the pathogenic landscape of human opisthorchis viverrini infections.201627926938
seasonal and spatial environmental influence on opisthorchis viverrini intermediate hosts, abundance, and distribution: insights on transmission dynamics and sustainable control.opisthorchis viverrini (ov) is a complex-life-cycle trematode affecting 10 million people in sea (southeast asia). human infection occurs when infected cyprinid fish are consumed raw or undercooked. ov requires three hosts and presents two free-living parasitic stages. as a consequence ov transmission and infection in intermediate and human hosts are strongly mediated by environmental factors and understanding how environmental variability influences intermediate host abundance is critical. the ...201627880787
induction of regulatory t cells by opisthorchis viverrini.opisthorchis viverrini causes public health problems in south-east asia. recently, tgf-β and il-10 have been reported to increase in o. viverrini-infected hamsters but the sources of these cytokines are still unknown. in this study, the cd4(+) t cells in infected hamsters were investigated. it was demonstrated that il-4(+) cd4(+) t cells were significantly increased in hamster spleens and mesenteric lymph nodes (mlns) during chronic infection. interestingly, il-10(+) cd4(+) t cells were also dis ...201627552546
health informatics model for helminthiasis in thailand.at the beginning of the new millennium, helminth infections continue to be prevalent, particularly among impoverished populations. this study attempts to create the first health informatics model of helminthiasis in thailand. the authors investigate how a health informatics model could be used to predict the control and eradication in a national control campaign. fish-borne helminthiasis caused by opisthorchis viverrini remains a major public health problem in many parts of south-east asia, incl ...201627666946
efficacious and safe dose of praziquantel for the successful treatment of feline reservoir hosts with opisthorchiasis.opisthorchiasis caused by opisthorchis viverrini is a major food-borne zoonosis in greater mekong sub-region. even though campaigns discouraging the consumption of raw fish have been launched to public, the disease still remains highly endemic. the unsuccessful eradication of the disease is probably because of the persistence of the parasite in animal reservoir hosts, particularly felids. praziquantel (pzq) is the drug of choice for morbidity control of opisthorchiasis in humans and animals. how ...201627576001
morbidity assessment of opisthorchis viverrini infection in rural laos: i. parasitological, clinical, ultrasonographical and biochemical findings.infections with the food-borne trematode opisthorchis viverinni are common in southeast asia. in lao pdr alone, two million people are supposed to be infected. opisthorchiasis may cause severe liver disease, eventually leading to cholangiocarcinoma. the objective of this study is to assess the eating habits, complaints, symptoms, signs and ultrasonographical findings in three different areas of savannakhet province.201627433131
single-ascending-dose pharmacokinetic study of tribendimidine in opisthorchis viverrini-infected patients.praziquantel is the only drug available for the treatment of opisthorchis viverrini infections. tribendimidine has emerged as a potential treatment alternative; however, its pharmacokinetic (pk) properties have not been sufficiently studied to date. via two phase iia dose-finding studies, 68 o. viverrini patients were treated with 25- to 600-mg doses of tribendimidine using 50- and 200-mg tablet formulations. plasma, blood, and dried blood spots (dbs) were sampled at selected time points. the tw ...201627431234
prevalence of opisthorchis viverrini-like fluke infection in ducks in binh dinh province, central vietnam.following the first report of opisthorchis viverrini infection in a domestic duck in phu my district of binh dinh province, central vietnam, many other cases were observed in the province. we determined the infection rate and intensity of o. viverrini infection in ducks in 4 districts of the province. a total of 178 ducks were randomly selected from 34 farms for examination of flukes in the liver and gall bladder. an infection rate of 34.3% (range 20.7-40.4% among districts) was found; the inten ...201627417094
opisthorchis viverrini infection among people in the border areas of three provinces, northeast of thailand.opisthorchis viverrini is still a serious problem in northeastern and northern thailand. active surveillance is required to determine updated data for further prevention and control planning. therefore, this study aimed to examine the prevalence and risk factors for o. viverrini in three provinces, northeastern thailand. a cross- sectional survey was conducted during october 2015 to march 2016 at kaeng sanam nang district of nakhon ratchasima province, waeng noi district of khon kaen province, a ...201627356720
infectivity and development of opisthorchis viverrini metacercariae in immunosuppressed barbonymus gonionotus fingerlings (cyprinidae).opisthorchiasis caused by opisthorchis viverrini infection, is a serious public health problem in lao pdr, cambodia, vietnam and thailand. cyprinid fish are required for the transmission of o. viverrini metacercariae to fish eating-mammal hosts including humans. the transmission of infective metacerariae is an important event, potentially resulting in the development of cholangiocarcinoma in cases of chronic opisthorchiasis. for a better understanding of the development of o. viverrini metacerca ...201627338181
evaluation of cholangiocarcinoma risk and its related factors in wetland geographical communities of ubon ratchathani, thailand.wetland geographical areas have a higher incidence of opisthorchis viverrini-associated cholangiocarcinoma (cca), confirmed by data from geographic information systems, than other areas. behavioral data also indicate that people in these areas traditionally eat uncooked freshwater fish dishes, a vehicle for o. viverrini infection. the best approach to reducing cca incidence is decreasing risk factors together with behavior alteration. evaluation of cca risk and its related factors are first need ...201627221857
characterization and differential expression analysis of toxocara canis aquaporin-1 gene.toxocara canis is an intestinal nematode of canids with a worldwide distribution, causing an important but neglected parasitic zoonosis in humans. aquaporins (aqp) are a family of water channel proteins, which function as membrane channels to regulate water homeostasis. in this study, the coding sequence of aquaporin-1 gene of t. canis (tc-aqp-1) was cloned and characterized. the obtained tc-aqp-1 coding sequence was 933 bp in length, which predicted to encode 311 amino acids. two conserved aspa ...201627215210
cercarial emergence patterns for opisthorchis viverrini sensu lato infecting bithynia siamensis goniomphalos from sakon nakhon province, thailand.opisthorchis viverrini sensu lato is a food-borne trematode which is classified as a class 1 carcinogen, with infection potentially leading to cholangiocarcinoma. snails of the genus bithynia act as the first intermediate hosts and an amplifying point in the parasite life cycle. in order to investigate seasonal effect on transmission dynamics of o. viverrini in bithynia siamensis goniomphalos, cercarial emergence and output profiles were monitored at different season. a total of 4533 snails orig ...201627154765
upregulation of endothelial nitric oxide synthase (enos) and its upstream regulators in opisthorchis viverrini associated cholangiocarcinoma and its clinical significance.endothelial nitric oxide synthase (enos) is an isoform of the enzyme nitric oxide synthase (nos) which is constitutively expressed in endothelial cells and plays important roles in vasodilation. we previously reported the importance of enos activation in cholangiocarcinoma (cca) tissues and cell lines. the present study aims to investigate the relative abundance of enos and phosphorylated enos (p-enos) and their upstream regulators vegfr3, vegfc, epha3 and ephrin-a1, in the opisthorchis viverrin ...201627143607
chicken igy-based coproantigen capture elisa for diagnosis of human opisthorchiasis.diagnosis of opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. therefore sensitive and specific diagnostic test is needed. in this study, a coproantigen sandwich elisa using recombinant o. viverrini cathepsin f (rov-cf) was developed. this sandwich elisa employing chicken igy raised against rov-cf in com ...201627140305
prevalence and factors associated with opisthorchis viverrini infection in khammouane province, lao pdr.opisthorchis viverrini (ov) liver flukes are common parasites found in central and southern laos and constitute a major public health problem in the country. laos people continue to have the habit of extensively consuming raw or half-cooked fish which are intermediate hosts. this study aimed to study the prevalence and factors associated with ov infection in the population of thakek district, khammouane province. this cross-sectional analytic study covered 237 subjects who filled out structured ...201627039810
plasma peptidome as a source of biomarkers for diagnosis of cholangiocarcinoma.cholangiocarcinoma (cca) is the bile duct cancer which constitutes one of the important public health problems in thailand with high mortality rate, especially in the opisthorchis viverrini (a parasite risk factor for cca) endemic area of the northeastern region of the country. this study aimed to identify potential biomarkers from the plasma peptidome by cca patients. peptides were isolated using 10 kda cut-off filter column and the flow-through was then used as a peptidome for lc-ms/ms analysi ...201627039742
nitrative and oxidative dna damage in infection-related carcinogenesis in relation to cancer stem cells.infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and nitrative dna lesions, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in inflammation-mediated carcinogenesis. it has bee ...201628050219
effects of fermentation time and low temperature during the production process of thai pickled fish (pla-som) on the viability and infectivity of opisthorchis viverrini metacercariae.contamination of a popular fermented fish dish, pla-som, by opisthorchis viverrini metacercariae (ovmc) is a possible cause of carcinogenic liver fluke infection in thailand. affected individuals are at risk of bile duct cancer, which is a major health problem for people in the greater mekong subregion. in order to investigate concerns about food safety, we studied the effects of fermentation time and low temperature on the viability and infectivity of ovmc during the pla-som production process. ...201626587967
development and validation of an enantioselective lc-ms/ms method for the analysis of the anthelmintic drug praziquantel and its main metabolite in human plasma, blood and dried blood spots.praziquantel (pzq) is the treatment of choice against various trematode and cestode infections. to study the pharmacokinetics of pzq in patients infected with the liver fluke opisthorchis viverrini, we developed and validated an enantioselective liquid chromatography coupled to tandem mass spectrometry method for the analysis of r - and s -pzq and its r -trans-4-oh-pzq metabolite in human plasma, blood and dried blood spots (dbs). the analytes were detected in the positive mode using selected re ...201626517852
immunization and challenge shown by hamsters infected with opisthorchis viverrini following exposure to gamma-irradiated metacercariae of this carcinogenic liver fluke.here we report findings to optimize and standardize conditions to attenuate metacercariae of opisthorchis viverrini by ionizing radiation to elicit protective immune responses to challenge infection. metacercariae were gamma-irradiated and the ability of irradiated metacercariae to prevent patent infection of challenge metacercariae in hamsters was determined, as well as their ability to induce a host antibody response. metacercariae irradiated in a dose-dependent manner, with 3, 5, 10, 12, 20, ...201625315797
changing patterns of prevalence in opisthorchis viverrini sensu lato infection in children and adolescents in northeast thailand.infection with the liver fluke opisthorchis viverrini sensu lato (s.l.), a group 1 carcinogen, is the most important risk factor for developing cholangiocarcinoma (cca) in southeast asia. cholangiocarcinoma is a fatal disease with the world's highest incidence being found in northeast thailand. liver fluke infection occurs through eating raw or partially cooked cyprinid fish containing metacercariae and, therefore, the control of o. viverrini s.l. infection should lead to a reduction in cca inci ...201627794488
cpg oligodeoxynucleotides with crude parasite antigens reduce worm recovery in opisthorchis viverrini infected hamsters.opisthorchis viverrini, a human liver fluke, is still an endemic parasitic infection in thailand and nearly all countries in southeast asia. o. viverrini induces a chronic stage of infection in hamsters. during the first 2 weeks of infection, th1 inducing cytokine, il-12, increased but was down regulated in chronic infection. in this study it was found that unmethylated-cpg odn (oligodeoxynucleotides) 1826 increased hamster mononuclear cell proliferation and stimulated ifn-γ production in vitro. ...201627737764
people participation towards opisthorchis viverrini prevention and control in chaiyaphum province, northeastern thailand.this cross-sectional descriptive and qualitative study was aimed to study the people participation and their approaches toward the human carcinogenic liver fluke, opisthorchis viverrini, prevention and control in ban chaun sub-district administrative organization (bcsao) and bamnet narong sub-district municipality (bnsm), bamnet narong district, chaiyaphum province, thailand between june 2013 and february 2014. participants were purposive selected, included chiefs of sub-district administrative ...201626838206
untangling the complexity of liver fluke infection and cholangiocarcinoma in ne thailand through transdisciplinary learning.this study demonstrates how a transdisciplinary learning approach provided new insights for explaining persistent opisthorchis viverrini infection in northern thailand, as well as elucidating problems of focusing solely on the parasite as a means of addressing high prevalence of cholangiocarcinoma. researchers from diverse backgrounds collaborated to design an investigative homestay program for 72 singaporean and thai university students in five northeast thai villages. the students explored how ...201626822781
xanthohumol inhibits stat3 activation pathway leading to growth suppression and apoptosis induction in human cholangiocarcinoma cells.stat3 plays a significant role in the development of cholangiocarcinoma (cca) associated with the liver fluke (opisthorchis viverrini; ov). xanthohumol (xn), a prenylated flavonoid extracted from hops, has known anticancer activity and could potentially target stat3. the present study determined the effect of xn on stat3, as well as ascertained its usefulness against cca. the cca cell proliferation at 20 µm and 50 µm of xn was shown to inhibited, while 20 µm partially inhibited il-6-induced stat ...201626794001
genomics of worms, with an emphasis on opisthorchis viverrini - opportunities for fundamental discovery and biomedical outcomes.neglected tropical diseases cause substantial morbidity and mortality in animals and people globally. opisthorchiasis is one such disease, caused by the carcinogenic, asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is known to be associated with malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia, including thailand, lao people's democratic republic (pdr) and cambodia. no vaccine is available, and only one drug (praziquantel) is routinely emplo ...201626792076
ultrasonographic features of hepatobiliary pathology in opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma.infection with the liver fluke, opisthorchis viverrini (ov), is an important public health problem in northeast thailand where people have a habit of eating raw or inadequately cooked fish. opisthorchiasis is associated with a number of asymptomatic hepatobiliary abnormalities and cholangiocarcinoma (cca) that can be observed conveniently by ultrasonography. based on our accumulated data, characteristic features of ultrasonographic findings of hepatobiliary pathology in ov infection are reviewed ...201627956092
efficacy and safety of praziquantel against light infections of opisthorchis viverrini: a randomised parallel single blind dose-ranging trial.the liver fluke opisthorchis viverrini, highly prevalent in southeast asia, is an important public health burden, including a risk factor for developing an aggressive bile duct cancer, cholangiocarcinoma, in chronically infected patients. praziquantel, administered at single 40 mg/kg in preventive chemotherapy programs and 3 x 25 mg/kg for individual treatment is the drug of choice, yet information on the nature of the dose-response relationship is lacking.201627927864
co-infections with liver fluke and helicobacter species: a paradigm change in pathogenesis of opisthorchiasis and cholangiocarcinoma?infection with the fish-borne liver fluke opisthorchis viverrini is classified by the international agency for research on cancer as a group 1 carcinogen: definitely carcinogenic in humans. cofactors likely contribute to bile duct cancer (cholangiocarcinoma) caused by this infection. here we review recent findings that address the role of liver fluke associated h. pylori in hepatobiliary disease and malignancy. we hypothesize that co-infection by o. viverrini and the bacillus helicobacter pylori ...201627919744
differential protein expression in the hemolymph of bithynia siamensis goniomphalos infected with opisthorchis viverrini.bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke opisthorchis viverrini. this parasite is a major public health problem in different countries throughout the greater mekong sub-region (thailand, southern vietnam, lao pdr and cambodia). chronic o. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma ...201627893749
the lawa model: a sustainable, integrated opisthorchiasis control program using the ecohealth approach in the lawa lake region of thailand.opisthorchiasis caused by the carcinogenic liver fluke opisthorchis viverrini is a major foodborne parasitic zoonotic disease in thailand and neighboring mekong countries. the infection is associated with several hepatobiliary diseases including cholangiocarcinoma (cca). the rates of cca in regions where the parasite is endemic are unprecedented. extensive research on various aspects of opisthorchiasis has been conducted in recent decades. however, the current status of o. viverrini infection in ...201627890720
upregulation of 14-3-3 eta in chronic liver fluke infection is a potential diagnostic marker of cholangiocarcinoma.to discover protein markers in chronic/advanced opisthorchiasis for the early detection of opisthorchis viverrini (ov)-associated cholangiocarcinoma (cca).201626435198
trematode diversity in the freshwater snail bithynia siamensis goniomphalos sensu lato from thailand and lao pdr.in order to obtain a comprehensive understanding of trematode diversity in bithynia siamensis goniomphalos sensu lato, the first intermediate host of the liver fluke opisthorchis viverrini s.l., the prevalence of larval trematode species was investigated in different localities in thailand and lao people's democratic republic (lao pdr). in thailand, snail samples were collected from 29 localities in the nine provinces: buri ram, surin, chaiya phum, maha sarakham, khon kaen, kalasin, mukdahan, sa ...201625997613
association between opisthorchis viverrini and leptospira spp. infection in endemic northeast thailand.opisthorchiasis caused by opisthorchis viverrini is an important foodborne trematodiasis in thailand, laos and cambodia. interestingly, the opisthorchiasis endemic region overlaps with an area of leptospirosis emergence. here we report an association between opisthorchiasis and leptospirosis in thailand. of 280 sera collected from villagers living around the lawa wetland complex in khon kaen province, 199 (71%) were seropositive for leptospirosis by immunochromatography. individuals with o. vive ...201627746382
modifying health behavior for liver fluke and cholangiocarcinoma prevention with the health belief model and social support theory.the liver fluke opisthorchis viverrini is a serious health problem in thailand. infection is associated with cholangiocarcinoma (cca), endemic among human populations in northeast and north thailand where raw fish containing fluke metacercariae are frequently consumed. recently, thailand public health authorities have been organized to reduce morbidity and mortality particularly in the northeast through o. viverrini and cca screening projects. health modfication is one of activities included in ...201627644606
efficacy and safety of tribendimidine against opisthorchis viverrini: two randomised, parallel-group, single-blind, dose-ranging, phase 2 trials.treatment of the liver fluke infection opisthorchis viverrini relies exclusively on praziquantel. tribendimidine could be an alternative treatment option. we aimed to assess the efficacy and safety of ascending single, oral doses of tribendimidine in patients with o viverrini infection.201627472949
population pharmacokinetic modeling of tribendimidine metabolites in opisthorchis viverrini-infected adults.there is a pressing need for alternative treatments against the liver fluke opisthorchis viverrini oral tribendimidine is a promising candidate, but its population pharmacokinetic properties are unknown. two phase iia trials were conducted in laos in o. viverrini-infected adults receiving single oral doses of 25 to 600 mg tribendimidine administered as different formulations in each study (study 1 used 200-mg tablets, and study 2 used 50-mg tablets). venous whole blood, plasma, and capillary dri ...201627431233
tissue microbiome profiling identifies an enrichment of specific enteric bacteria in opisthorchis viverrini associated cholangiocarcinoma.cholangiocarcinoma (cca) is the primary cancer of the bile duct system. the role of bile duct tissue microbiomes in cca tumorigenesis is unestablished. to address this, sixty primary cca tumors and matched normals, from both liver fluke (opisthorchis viverrini) associated (ova, n=28) and non-o. viverrini associated (non-ova, n=32) cancers, were profiled using high-throughput 16s rrna sequencing. a distinct, tissue-specific microbiome dominated by the bacterial families dietziaceae, pseudomonadac ...201627428430
a combination of liver fluke infection and traditional northeastern thai foods associated with cholangiocarcinoma development.opisthorchis viverrini infection is one of the risk factors for cholangiocarcinoma (cca) in northeast thailand, a region with one of the highest reported incidence rates of cca. the traditional practice of eating raw fish, repeated exposure to liver flukes, and consumption of nitrosamine-contaminated food are major risk factors for cca. so far, there have been no reports about which northeastern traditional dishes may be involved in cca development. the present study, thus, investigated the effe ...201627271702
overweight relation to liver fluke infection among rural participants from 4 districts of nakhon ratchasima province, thailand.a cross-sectional survey was conducted among 730 participants from 4 rural districts of nakhon ratchasima province, thailand, with a reported high incidence of liver fluke infection. this study was aimed to examine and evaluate the nutritional status in relation to opisthorchis viverrini infection. participants were purposive selected from chum phuang, mueang yang, bua yai, and kaeng sanam nang districts. stool samples were prepared by kato katz technique and then assessed by microscopy. anthrop ...201627268631
assessing the role of landscape connectivity on opisthorchis viverrini transmission dynamics.opisthorchis viverrini (ov) is one of the most important human parasitic diseases in southeast asia. although the concept of connectivity is widely used to comprehend disease dispersal, knowledge of the influences of landscape connectivity on ov transmission is still rudimentary. this study aimed to investigate the role of landscape connectivity in ov transmission between the human and the first intermediate snail hosts. fieldwork was conducted in three villages respectively in kamalasai distric ...201627265875
cd44 variant-dependent redox status regulation in liver fluke-associated cholangiocarcinoma: a target for cholangiocarcinoma treatment.expression of cd44, especially the variant isoforms (cd44v) of this major cancer stem cell marker, contributes to reactive oxygen species (ros) defense through stabilizing xct (a cystine-glutamate transporter) and promoting glutathione synthesis. this enhances cancer development and increases chemotherapy resistance. we investigate the role of cd44v in the regulation of the ros defense system in cholangiocarcinoma (cca). immunohistochemical staining of cd44v and p38(mapk) (a major ros target) ex ...201627176078
pharmacokinetic study of praziquantel enantiomers and its main metabolite r-trans-4-oh-pzq in plasma, blood and dried blood spots in opisthorchis viverrini-infected patients.praziquantel (pzq) is the treatment of choice for infections with the liver fluke opisthorchis viverrini, a major health problem in southeast asia. however, pharmacokinetic (pk) studies investigating the disposition of pzq enantiomers (r- and s-pzq) and its main metabolite, r-trans-4-oh-pzq, in diseased patients are lacking. the implementation of a dried blood spot (dbs) sampling technique would ease the performance of pk studies in remote areas without clinical facilities. the aim of the presen ...201627152952
decreased risk of cholangiocarcinogenesis following repeated cycles of opisthorchis viverrini infection-praziquantel treatment: magnetic resonance imaging (mri) and histopathological study in a hamster model.it has been suggested that repeated infection of opisthorchis viverrini followed by repeated treatment with praziquantel (pzq) increases risk of development of cholangiocarcinoma (cca). evidence for the prediction has accumulated based on findings of indirect approaches involving molecular changes and epidemiological trends. by contrast, here we directly monitored the impact of repeated liver fluke infection and treatment with pzq on cholangiocarcinogenesis in a rodent model of human opisthorchi ...201627143606
wound healing and cancer progression in opisthorchis viverrini associated cholangiocarcinoma.infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). it was shown previously that o. viverrini-secreted proteins accelerate wound resolution in human cholangiocytes. recombinant ov-grn-1 (o. viverrini-derived gene encoding granulin-like growth factor) induced angiogenesis and accelerated mouse wound healing. given the striking similarities of wound healing and cancer progression, here we discuss the major implications of this fin ...201627130317
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